MS015078 (gene) Bitter gourd (TR) v1

Overview
NameMS015078
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionU4/U6 small nuclear ribonucleoprotein Prp31 homolog
Locationscaffold2: 1002991 .. 1005646 (-)
RNA-Seq ExpressionMS015078
SyntenyMS015078
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGTAAGTAATGAATTTTACTTTCCTTTCTGGAAAGCAAAGTCATAATAATGCTATTAGTAGTGTAAATTCATCTACCGTATATTTTGAGGCAATAGTTATTGGCTGTCTACAGGATGAAGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCTGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCGAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGGTTAGTTTACATGAAAATTTCCTTATTGATTAGGGCATATGCCGTGGCTCGAAGATAAAAGGGTTGGAAGAAACCCAATGCATGCGTTGGATCCCTGATGTCTTGTCTATTTTTTAACCTTTTATTGAATGATAAGTTATGGCTTATGGGTTATTGTAAAATTGTTGGAAGTGTCTTCTTGGCAATTCATTGTTTCTAAATAATATTACATTTGAAGAGTTTGAAAATCTATGCAAGTTATGATGACCGTTTCAATTTTACTATCCACAACAATGATGTTCCTTGAGCCATAACTCCTCTATTTGAAATGTTAAATATTTTAGTGTGCATACATGATATTATTTGTGATGCCTCATTTCCAAGTCACTAACTTTTCTGTTGCCATCTGAATGACAGAAAGTGGAAGATGCATTGCAGAAGGATTCTAATATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAAAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCCGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAGGAACCTCCACCTGCAAAACAACCGAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGGTACTGATATGTTACATGTGTCTTTCATCTCTCTAACTATAGAAGCTTTGATTTGTTGCAGTTTTTGGTGTTCATGGATTGATGTGATTTTCCTTGTCAGATATGCAACAACGGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGTAAAGCATTCTTTAAATTTTGAATAATCTGGATAGATGAAATTTTAGTCCTGCTTCTGCTTCATTTCACATTACACGTCAGTATGGATGCAATGTTGGGTCGTTCAATAAGCATGAGTTGCCAAGTTGTCTGGTGAACCAGTAAATTTTCTAAATTGCAAGTAACACTACCATAACCGTCTTTCCCTCTCTTGAAGAAATATTTCATATCAGAATTGGTTTTATTTTCTTCTGAATACCAGCATATTTAGAAATCTGTTCTGGATGGAAAAAGTAGTTGTTTGAGCATTCTGCAATAATATTCTGAACAAACATTTCACTTTCTTCATTCTTTCTGCACATAAAACAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAAGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTAAGTCATGGCATTGAGCTGGTCCTAATATCTTTCACACACAAAATTCTCCCCACCACAGATATCTGCAGCTCACTATCGGGTTATTTTTTATATTTTCTTTCTAAATTACTTTCAGGTTCAAGGAAAAACGTTATGGTAGCAGTGGCGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGTCAGTATTTCTTGCGTTCCTCATCATATCTTCCTATGTTATCTAAGTTAGCCTCGAAAGTAATTGTCTTATTCATGACACTGCCCAGATGCTTAATTTCTAGGTTGTAAGGATTTCCTGTTTTCCTTGCAGGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAACTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

mRNA sequence

GCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGATGAAGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCTGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCGAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGAAAGTGGAAGATGCATTGCAGAAGGATTCTAATATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAAAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCCGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAGGAACCTCCACCTGCAAAACAACCGAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACGGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAAGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGTAGCAGTGGCGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAACTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

Coding sequence (CDS)

GCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGATGAAGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCTGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCGAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGAAAGTGGAAGATGCATTGCAGAAGGATTCTAATATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAAAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCCGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAGGAACCTCCACCTGCAAAACAACCGAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACGGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAAGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGTAGCAGTGGCGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAACTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

Protein sequence

ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKIRKN
Homology
BLAST of MS015078 vs. NCBI nr
Match: XP_022151138.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 901.7 bits (2329), Expect = 2.7e-258
Identity = 482/483 (99.79%), Postives = 482/483 (99.79%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 2   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA
Sbjct: 182 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. NCBI nr
Match: XP_022945419.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022945420.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >KAG6573747.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.9 bits (2293), Expect = 4.0e-254
Identity = 472/483 (97.72%), Postives = 479/483 (99.17%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+Q
Sbjct: 2   ATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY+DIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL +
Sbjct: 182 EEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLGS 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. NCBI nr
Match: KAG7032269.1 (U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.1 bits (2291), Expect = 6.8e-254
Identity = 472/483 (97.72%), Postives = 475/483 (98.34%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 64  ATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 123

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 124 RYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 183

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 184 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 243

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL A
Sbjct: 244 EEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLVA 303

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 304 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 363

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 364 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 423

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 424 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 483

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 484 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 543

Query: 481 RKN 484
           RKN
Sbjct: 544 RKN 546

BLAST of MS015078 vs. NCBI nr
Match: XP_022956658.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moschata] >XP_023004221.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita maxima] >XP_023526610.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita pepo subsp. pepo] >KAG6601489.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.1 bits (2291), Expect = 6.8e-254
Identity = 472/483 (97.72%), Postives = 475/483 (98.34%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 2   ATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL A
Sbjct: 182 EEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLVA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. NCBI nr
Match: XP_022966885.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima])

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-253
Identity = 471/483 (97.52%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+Q
Sbjct: 2   ATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY+DIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA+
Sbjct: 182 EEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAS 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. ExPASy Swiss-Prot
Match: Q8RXN6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3702 GN=PRP31 PE=1 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 3.8e-199
Identity = 375/484 (77.48%), Postives = 421/484 (86.98%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATL DSFLADLDELSD +  LDE D D    EED+D D+ADLE+LNYDDLD+VSKLQK+Q
Sbjct: 2   ATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DKY
Sbjct: 62  RYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           +LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  LP
Sbjct: 122 KLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSALP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           E++LQK ++ACDRAL LDSA+KKVL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+A
Sbjct: 182 EDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLSA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RL+AAKST
Sbjct: 242 LAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRLR
Sbjct: 302 LAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA--A 420
           KMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   A
Sbjct: 362 KMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKINA 421

Query: 421 KVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFS 480
           KV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  L  LGSGTQSTYFSE+GTFS
Sbjct: 422 KVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTFS 481

Query: 481 KIRK 483
           K++K
Sbjct: 482 KLKK 484

BLAST of MS015078 vs. ExPASy Swiss-Prot
Match: Q8CCF0 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 PE=1 SV=3)

HSP 1 Score: 386.3 bits (991), Expect = 4.9e-106
Identity = 234/496 (47.18%), Postives = 332/496 (66.94%), Query Frame = 0

Query: 2   TLADSFLADLDELSDED---NFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQ 61
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + S++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKSIAKLW 61

Query: 62  KTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIR 121
            ++ + +IM K+E+ + K +N+S     +E  PEY++IV+ N+L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 122 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 181
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 182 GKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 241
           G+ L +E L++  +ACD AL L+++K ++  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 242 GGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLI 301
           GGL  L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RL+
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 302 AAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 361
           AAK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 362 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 421
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 422 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSG 481
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of MS015078 vs. ExPASy Swiss-Prot
Match: Q5U5C5 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.1e-105
Identity = 234/495 (47.27%), Postives = 329/495 (66.46%), Query Frame = 0

Query: 2   TLADSFLADLDELS--DEDNFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQK 61
           +LAD  LADL+E +  +E+N +DE+D++  E ++E++  D      LN + + S++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVDEEMQVD------LNAESVKSIAKLSD 61

Query: 62  TQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRD 121
           ++ +++I+ K+E  +QK    S     +E  PEY++IV+ N+L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIEGYIQKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 122 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 181
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 182 KPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 241
           + L +E L++  +ACD AL L+ +K ++  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 242 GLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIA 301
           GL  L+KMPACNV LLGA++K L GFS+ +     GYI  +EI QS P  L  +A RL++
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLTGFSSTSVLPHTGYIYHSEIVQSLPSDLHRKAARLVS 301

Query: 302 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 361
           AK TLA+RVDS   +P GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLASRVDSFHENPEGKIGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 362 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 421
           RR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 422 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSGT 481
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTVRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of MS015078 vs. ExPASy Swiss-Prot
Match: Q8WWY3 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 7.1e-105
Identity = 233/496 (46.98%), Postives = 330/496 (66.53%), Query Frame = 0

Query: 2   TLADSFLADLDELSDED---NFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQ 61
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + +++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKTIAKLW 61

Query: 62  KTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIR 121
            ++ + +IM K+E+ + K +  S     +E  PEY++IV+ N+L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 122 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 181
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 182 GKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 241
           G+ L EE L++  +ACD AL L+++K ++  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 242 GGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLI 301
           GGL  L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RL+
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 302 AAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 361
           AAK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 362 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 421
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 422 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSG 481
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of MS015078 vs. ExPASy Swiss-Prot
Match: Q6NVP6 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.7e-104
Identity = 231/495 (46.67%), Postives = 328/495 (66.26%), Query Frame = 0

Query: 2   TLADSFLADLDELS--DEDNFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQK 61
           +LAD  LADL+E +  +E+N +DE+D++  E ++E++  D      LN + + S++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVQEEMQVD------LNAESVKSIAKLSD 61

Query: 62  TQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRD 121
           ++ +++I+ K++  ++K    S     +E  PEY++IV+ N+L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIDGYIKKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 122 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 181
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 182 KPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 241
           + L +E L++  +ACD AL L+ +K ++  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 242 GLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIA 301
           GL  L+KMPACNV LLGA++K L+GFS+ +     GYI  ++I QS PP L  +A RL++
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLHRKAARLVS 301

Query: 302 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 361
           AK TLAARVDS      GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLAARVDSFHESSEGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 362 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 421
           RR RKMKER   TE+RK ANRM F   EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFAEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 422 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSGT 481
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of MS015078 vs. ExPASy TrEMBL
Match: A0A6J1DBC9 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019139 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.3e-258
Identity = 482/483 (99.79%), Postives = 482/483 (99.79%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 2   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA
Sbjct: 182 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. ExPASy TrEMBL
Match: A0A6J1G0W2 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449657 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 1.9e-254
Identity = 472/483 (97.72%), Postives = 479/483 (99.17%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+Q
Sbjct: 2   ATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY+DIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL +
Sbjct: 182 EEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLGS 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. ExPASy TrEMBL
Match: A0A6J1KRI6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497587 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.3e-254
Identity = 472/483 (97.72%), Postives = 475/483 (98.34%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 2   ATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL A
Sbjct: 182 EEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLVA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. ExPASy TrEMBL
Match: A0A6J1GXP7 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458320 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.3e-254
Identity = 472/483 (97.72%), Postives = 475/483 (98.34%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 2   ATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RYNDIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL A
Sbjct: 182 EEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLVA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. ExPASy TrEMBL
Match: A0A6J1HSU1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466452 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.6e-254
Identity = 471/483 (97.52%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+Q
Sbjct: 2   ATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY+DIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 62  RYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           EEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA+
Sbjct: 182 EEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAS 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKST
Sbjct: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 420
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421

Query: 421 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 480
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSKI
Sbjct: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSKI 481

Query: 481 RKN 484
           RKN
Sbjct: 482 RKN 484

BLAST of MS015078 vs. TAIR 10
Match: AT1G60170.1 (pre-mRNA processing ribonucleoprotein binding region-containing protein )

HSP 1 Score: 695.7 bits (1794), Expect = 2.7e-200
Identity = 375/484 (77.48%), Postives = 421/484 (86.98%), Query Frame = 0

Query: 1   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 60
           ATL DSFLADLDELSD +  LDE D D    EED+D D+ADLE+LNYDDLD+VSKLQK+Q
Sbjct: 2   ATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKSQ 61

Query: 61  RYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 120
           RY DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DKY
Sbjct: 62  RYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDKY 121

Query: 121 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 180
           +LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  LP
Sbjct: 122 KLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSALP 181

Query: 181 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 240
           E++LQK ++ACDRAL LDSA+KKVL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+A
Sbjct: 182 EDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLSA 241

Query: 241 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 300
           LAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RL+AAKST
Sbjct: 242 LAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKST 301

Query: 301 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 360
           LAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRLR
Sbjct: 302 LAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRLR 361

Query: 361 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA--A 420
           KMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   A
Sbjct: 362 KMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKINA 421

Query: 421 KVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFS 480
           KV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  L  LGSGTQSTYFSE+GTFS
Sbjct: 422 KVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTFS 481

Query: 481 KIRK 483
           K++K
Sbjct: 482 KLKK 484

BLAST of MS015078 vs. TAIR 10
Match: AT1G70400.1 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 479 Blast hits to 479 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 125; Fungi - 138; Plants - 124; Viruses - 0; Other Eukaryotes - 92 (source: NCBI BLink). )

HSP 1 Score: 214.2 bits (544), Expect = 2.4e-55
Identity = 118/172 (68.60%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 38  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 97
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 98  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 157
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 158 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKKVLNFVE 210
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KKVL FV+
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKKVLEFVD 162

BLAST of MS015078 vs. TAIR 10
Match: AT1G70400.2 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 206.1 bits (523), Expect = 6.5e-53
Identity = 114/166 (68.67%), Postives = 133/166 (80.12%), Query Frame = 0

Query: 38  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 97
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 98  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 157
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 158 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KK
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKK 156

BLAST of MS015078 vs. TAIR 10
Match: AT1G70400.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: egg cell; CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 484 Blast hits to 484 proteins in 190 species: Archae - 0; Bacteria - 0; Metazoa - 149; Fungi - 134; Plants - 124; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink). )

HSP 1 Score: 206.1 bits (523), Expect = 6.5e-53
Identity = 114/166 (68.67%), Postives = 133/166 (80.12%), Query Frame = 0

Query: 38  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 97
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 98  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 157
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 158 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KK
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKK 156

BLAST of MS015078 vs. TAIR 10
Match: AT3G05060.1 (NOP56-like pre RNA processing ribonucleoprotein )

HSP 1 Score: 100.9 bits (250), Expect = 2.9e-21
Identity = 70/239 (29.29%), Postives = 118/239 (49.37%), Query Frame = 0

Query: 78  NISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDY 137
           +++   L    D    +I++   L  D++ E+      +R+ Y   FPEL  ++   I Y
Sbjct: 144 SLARYKLKFSSDKVDTMIIQAIGLLDDLDKELNTYAMRVREWYGWHFPELAKIISDNILY 203

Query: 138 ARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALAL 197
           A+ VK +GN ++   +D   +L   +   +   A  + G  + +  L    + CD+ L+L
Sbjct: 204 AKSVKLMGNRVNAAKLDFSEILADEIEADLKDAAVISMGTEVSDLDLLHIRELCDQVLSL 263

Query: 198 DSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAALAKMPACNVQLLGAKKK 257
              + ++ ++++SRM  IAPNL+A+VG  V A+L+   G L  L+K P   VQ+LGA+K 
Sbjct: 264 SEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLISHGGSLLNLSKQPGSTVQILGAEKA 323

Query: 258 NLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKSTLAARVDSTRGDPTGKT 317
                 T  +  + G I    +     P  K +  R +AAK+ LA RVD+  GD    T
Sbjct: 324 LFRALKTKHATPKYGLIFHASLVGQAAPKHKGKISRSLAAKTVLAIRVDAL-GDSQDNT 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151138.12.7e-25899.79U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charan... [more]
XP_022945419.14.0e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022... [more]
KAG7032269.16.8e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita arg... [more]
XP_022956658.16.8e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moscha... [more]
XP_022966885.12.0e-25397.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RXN63.8e-19977.48U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3... [more]
Q8CCF04.9e-10647.18U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 P... [more]
Q5U5C51.1e-10547.27U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 ... [more]
Q8WWY37.1e-10546.98U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE... [more]
Q6NVP62.7e-10446.67U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prp... [more]
Match NameE-valueIdentityDescription
A0A6J1DBC91.3e-25899.79U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica char... [more]
A0A6J1G0W21.9e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=366... [more]
A0A6J1KRI63.3e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxi... [more]
A0A6J1GXP73.3e-25497.72U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita mosc... [more]
A0A6J1HSU19.6e-25497.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT1G60170.12.7e-20077.48pre-mRNA processing ribonucleoprotein binding region-containing protein [more]
AT1G70400.12.4e-5568.60CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.26.5e-5368.67CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.36.5e-5368.67FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G05060.12.9e-2129.29NOP56-like pre RNA processing ribonucleoprotein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012976NOSICSMARTSM00931NOSIC_2coord: 94..146
e-value: 4.8E-23
score: 92.6
IPR019175Prp31 C-terminalPFAMPF09785Prp31_Ccoord: 338..455
e-value: 3.8E-43
score: 147.3
IPR002687Nop domainPFAMPF01798Nopcoord: 102..330
e-value: 1.1E-73
score: 247.5
IPR002687Nop domainPROSITEPS51358NOPcoord: 215..333
score: 37.543999
IPR042239Nop, C-terminal domainGENE3D1.10.246.90Nop domaincoord: 216..341
e-value: 1.1E-40
score: 140.3
NoneNo IPR availableGENE3D1.10.287.4070coord: 76..215
e-value: 3.5E-57
score: 194.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 329..360
NoneNo IPR availablePANTHERPTHR13904:SF0U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31coord: 1..480
IPR027105U4/U6 small nuclear ribonucleoprotein Prp31PANTHERPTHR13904PRE-MRNA SPLICING FACTOR PRP31coord: 1..480
IPR036070Nop domain superfamilySUPERFAMILY89124Nop domaincoord: 87..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015078.1MS015078.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
biological_process GO:0000244 spliceosomal tri-snRNP complex assembly
cellular_component GO:0046540 U4/U6 x U5 tri-snRNP complex