MS015065 (gene) Bitter gourd (TR) v1

Overview


Name	MS015065
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	proline-rich receptor-like protein kinase PERK13
Location	scaffold2: 912927 .. 915506 (-)
RNA-Seq Expression	MS015065
Synteny	MS015065

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCCACCTTCTCCAAAACCTGAGAATCGCTCAGCTCCGACAGATAACGGCAACGACGCTCCGGATCCTGACCCCGATGCAGAGCCCCAAACATCTGAATCCTCGAACCCTACTCCACCAGAAGGAGATCCAGACACGATTCCTTCCACAGGAGCAAGCCCGAAGACGACGAAACAAATTCCTAGTCCTCCTGGAGAAATGAGTACTACCTCAGTATCACCGGGATCTAATTTTCTTCCTCCATCCACGGATACAAACGCAGACTCAGATACCAACGCAGACGCAGCAGACGCAGACCCGGCCACAGGCACGAGCACAACCACAACCACAACCACACCCACACCCACTCTTACTCCAAACAACCCATCTCCTTCCTCCGACTCAAAACCATTGAAATCGTCAACAGGGGAGCCCCATACACCACCCTCGGGTTCATCTGGTAACACTCAAGTTGCGGTAGGTGCTGCAATAGCTGGAGTTTTGTTAATTATTTTGGTTGCCGTGCTTTTTGTTTTCTCAAGGAAGAAGAAAAGACGGGGAGATTTATATCCCGGCCCCTACATGCCTCCTAACAACTTCTGTGTGAAATCAGGTACTGGAGTTATCCAAATTAGCCCATTGTTGAATATATAAAGAAACTTGTTATATGGTTCTGATGTGGTGATGATGCTAATTTTCTTACTTGCAGATGGAAATTACTATCCCCATCAACATATGGGCAACTCTGGTTCGCCAGAAGGCTTCTATACTCAGGTACCGCATACTCCTGTTGGAAACAGCTTTGGGAGTCAAAAAGGAACCGTATACAGTGGCAGTGGAGTGGAGTCGGGGGTTATAAGCACCGCTAAGTTCTTCTTCAGCTATGAGGAGTTGATGGAAATTACGTCTGGATTTTCGCGTCAAAACATTCTTGGGGAAGGTGGGTTTGGATGTGTTTTCAAGGGTTGGCTTCCAGAAGGGAAGCCAGTGGCTGTGAAGCAACTCAAGGCAGGAAGTGGACAGGGGGAGAGGGAATTCAAGGCCGAAGTCGAGATTATCAGTCGTGTTCATCATCGACATTTGGTGTCTTTAGTGGGCTACTGCGTCTCAGAGAATCATAGATTGCTCATCTATGAGTTTGTTCCTAATATGACTCTTGAGCATCATCTCCATAGCAGTATGGAACCCATCATTGATCTCCAACATCACTTTAAAATAGCTTTATGAGGAGGTGTTGGTGTTGATAATATTTGACTTCTCTGGGCAGGTAAAGGAGTCCCTGTGTTGGATTGGTCTAAAAGACTCAAAATTGCTTTGGGAGCTGCAAAGGGTTTGGCATATCTGCATGAGGATTGTGAGTACATATCAGTAAACTCAAAAAAGTTTTGCAGAATTGGTCTGTTTCAATATTTCTAAGACGACATATTCCATTAACTCCATATCTCCTTTTGTTGTCCTGTTTCATAGGTCATCCCAAGATTATCCACAGAGACATCAAGTCAGCCAACATTTTACTGGATGATGCTTTTGAGGCACAGGTACGTCCACACCAATTTTTTCTAACAATTTCGAAACCATACATGCTATTTTCTTATTTCCAGAGTCTCTGGTTCCAATTTCGATGGACAACTTCAAACACAGGTTGCAGATTTTGGACTTGCGAAACTGACGAATGACACACACACCCACGTATCGACTCGTGTCATGGGGACATTTGGGTAGGTTAACTCGACATTTACGCATCACATAACAATTCTTTGGGCCATTTTTGTTCCATATAAATTGATCGTTTGTGTTTCAATGGCTTGCAGATACATGGCACCTGAATACGCATCAAGTGGGAAATTGACAGACAGATCAGATGTATTCTCGTTTGGGGTTGTGCTTCTTGAGCTTATTACTGGTCGTAAGCCTGTTGATCCTTCTCAACCTTTGGGGGATGAGAGTTTGGTCGAATGGGTACGTAAATAAATGTACAAACCTTTCAAATTTCCTCTGCATTTTCTGTTACATTTCTTGTCGAGCCATGTGGTATTGAAGATCTTTCTGCTCCCTTGAGATGCACTTGAATAGATTCAGCAATGTTCTATGTTATGGATGTTGCAGGCTCGTCCACACCTCATACGTGCCCTTGAAACTGGGGAGTTCGGCGAATTGACAGATCCACGACTTGAAAAACATTACGTGGAAAGTGAAATGTTCAGAATGATCGAAGCAGCAGCTGCCTGCGTTCGTCATTCGGCTCCCAAACGGCCTCGCATGGTTCAGGTTAACATAGTTCTATTCTGAACTGCAGTTTGGATAACCGTGTTCTTAAATTGTCATTCTTATTAACAGGTGGTGAGGGCACTAGATACTGAAAGTGACATGTCCGACCTCTCCAATGGTGTCAAATTTGGCCAGAGTACCATCTATGATTCTGGACAATACAGTCAAGACATTTCTAGATTCAGGAGAATGGCACTCGGTGGAGACAGCTCCGAATTCGACGTTTATAGTAGCGAATACAATTCAAGAGAAATGACTGCAAGTCAAGAATCATGGAGATACCAAGATAACTCAAGTGGTGAACAAGAAACTCGAGCCTTTAAAGGACAAAGT

mRNA sequence

AGTCCACCTTCTCCAAAACCTGAGAATCGCTCAGCTCCGACAGATAACGGCAACGACGCTCCGGATCCTGACCCCGATGCAGAGCCCCAAACATCTGAATCCTCGAACCCTACTCCACCAGAAGGAGATCCAGACACGATTCCTTCCACAGGAGCAAGCCCGAAGACGACGAAACAAATTCCTAGTCCTCCTGGAGAAATGAGTACTACCTCAGTATCACCGGGATCTAATTTTCTTCCTCCATCCACGGATACAAACGCAGACTCAGATACCAACGCAGACGCAGCAGACGCAGACCCGGCCACAGGCACGAGCACAACCACAACCACAACCACACCCACACCCACTCTTACTCCAAACAACCCATCTCCTTCCTCCGACTCAAAACCATTGAAATCGTCAACAGGGGAGCCCCATACACCACCCTCGGGTTCATCTGGTAACACTCAAGTTGCGGTAGGTGCTGCAATAGCTGGAGTTTTGTTAATTATTTTGGTTGCCGTGCTTTTTGTTTTCTCAAGGAAGAAGAAAAGACGGGGAGATTTATATCCCGGCCCCTACATGCCTCCTAACAACTTCTGTGTGAAATCAGGTACTGGAGTTATCCAAATTAGCCCATTAAACTTGTTATATGGTTCTGATGTGGTGATGATGCTAATTTTCTTACTTGCAGATGGAAATTACTATCCCCATCAACATATGGGCAACTCTGGTTCGCCAGAAGGCTTCTATACTCAGGTACCGCATACTCCTGTTGGAAACAGCTTTGGGAGTCAAAAAGGAACCGTATACAGTGGCAGTGGAGTGGAGTCGGGGGTTATAAGCACCGCTAAGTTCTTCTTCAGCTATGAGGAGTTGATGGAAATTACGTCTGGATTTTCGCGTCAAAACATTCTTGGGGAAGGTGGGTTTGGATGTGTTTTCAAGGGTTGGCTTCCAGAAGGGAAGCCAGTGGCTGTGAAGCAACTCAAGGCAGGAAGTGGACAGGGGGAGAGGGAATTCAAGGCCGAAGTCGAGATTATCAGTCGTGTTCATCATCGACATTTGGTGTCTTTAGTGGGCTACTGCGTCTCAGAGAATCATAGATTGCTCATCTATGAGTTTGTTCCTAATATGACTCTTGAGCATCATCTCCATAGCAGTAAAGGAGTCCCTGTGTTGGATTGGTCTAAAAGACTCAAAATTGCTTTGGGAGCTGCAAAGGGTTTGGCATATCTGCATGAGGATTGTCATCCCAAGATTATCCACAGAGACATCAAGTCAGCCAACATTTTACTGGATGATGCTTTTGAGGCACAGGTTGCAGATTTTGGACTTGCGAAACTGACGAATGACACACACACCCACGTATCGACTCGTGTCATGGGGACATTTGGATACATGGCACCTGAATACGCATCAAGTGGGAAATTGACAGACAGATCAGATGTATTCTCGTTTGGGGTTGTGCTTCTTGAGCTTATTACTGGTCGTAAGCCTGTTGATCCTTCTCAACCTTTGGGGGATGAGAGTTTGGTCGAATGGGCTCGTCCACACCTCATACGTGCCCTTGAAACTGGGGAGTTCGGCGAATTGACAGATCCACGACTTGAAAAACATTACGTGGAAAGTGAAATGTTCAGAATGATCGAAGCAGCAGCTGCCTGCGTTCGTCATTCGGCTCCCAAACGGCCTCGCATGGTTCAGGTGGTGAGGGCACTAGATACTGAAAGTGACATGTCCGACCTCTCCAATGGTGTCAAATTTGGCCAGAGTACCATCTATGATTCTGGACAATACAGTCAAGACATTTCTAGATTCAGGAGAATGGCACTCGGTGGAGACAGCTCCGAATTCGACGTTTATAGTAGCGAATACAATTCAAGAGAAATGACTGCAAGTCAAGAATCATGGAGATACCAAGATAACTCAAGTGGTGAACAAGAAACTCGAGCCTTTAAAGGACAAAGT

Coding sequence (CDS)

AGTCCACCTTCTCCAAAACCTGAGAATCGCTCAGCTCCGACAGATAACGGCAACGACGCTCCGGATCCTGACCCCGATGCAGAGCCCCAAACATCTGAATCCTCGAACCCTACTCCACCAGAAGGAGATCCAGACACGATTCCTTCCACAGGAGCAAGCCCGAAGACGACGAAACAAATTCCTAGTCCTCCTGGAGAAATGAGTACTACCTCAGTATCACCGGGATCTAATTTTCTTCCTCCATCCACGGATACAAACGCAGACTCAGATACCAACGCAGACGCAGCAGACGCAGACCCGGCCACAGGCACGAGCACAACCACAACCACAACCACACCCACACCCACTCTTACTCCAAACAACCCATCTCCTTCCTCCGACTCAAAACCATTGAAATCGTCAACAGGGGAGCCCCATACACCACCCTCGGGTTCATCTGGTAACACTCAAGTTGCGGTAGGTGCTGCAATAGCTGGAGTTTTGTTAATTATTTTGGTTGCCGTGCTTTTTGTTTTCTCAAGGAAGAAGAAAAGACGGGGAGATTTATATCCCGGCCCCTACATGCCTCCTAACAACTTCTGTGTGAAATCAGGTACTGGAGTTATCCAAATTAGCCCATTAAACTTGTTATATGGTTCTGATGTGGTGATGATGCTAATTTTCTTACTTGCAGATGGAAATTACTATCCCCATCAACATATGGGCAACTCTGGTTCGCCAGAAGGCTTCTATACTCAGGTACCGCATACTCCTGTTGGAAACAGCTTTGGGAGTCAAAAAGGAACCGTATACAGTGGCAGTGGAGTGGAGTCGGGGGTTATAAGCACCGCTAAGTTCTTCTTCAGCTATGAGGAGTTGATGGAAATTACGTCTGGATTTTCGCGTCAAAACATTCTTGGGGAAGGTGGGTTTGGATGTGTTTTCAAGGGTTGGCTTCCAGAAGGGAAGCCAGTGGCTGTGAAGCAACTCAAGGCAGGAAGTGGACAGGGGGAGAGGGAATTCAAGGCCGAAGTCGAGATTATCAGTCGTGTTCATCATCGACATTTGGTGTCTTTAGTGGGCTACTGCGTCTCAGAGAATCATAGATTGCTCATCTATGAGTTTGTTCCTAATATGACTCTTGAGCATCATCTCCATAGCAGTAAAGGAGTCCCTGTGTTGGATTGGTCTAAAAGACTCAAAATTGCTTTGGGAGCTGCAAAGGGTTTGGCATATCTGCATGAGGATTGTCATCCCAAGATTATCCACAGAGACATCAAGTCAGCCAACATTTTACTGGATGATGCTTTTGAGGCACAGGTTGCAGATTTTGGACTTGCGAAACTGACGAATGACACACACACCCACGTATCGACTCGTGTCATGGGGACATTTGGATACATGGCACCTGAATACGCATCAAGTGGGAAATTGACAGACAGATCAGATGTATTCTCGTTTGGGGTTGTGCTTCTTGAGCTTATTACTGGTCGTAAGCCTGTTGATCCTTCTCAACCTTTGGGGGATGAGAGTTTGGTCGAATGGGCTCGTCCACACCTCATACGTGCCCTTGAAACTGGGGAGTTCGGCGAATTGACAGATCCACGACTTGAAAAACATTACGTGGAAAGTGAAATGTTCAGAATGATCGAAGCAGCAGCTGCCTGCGTTCGTCATTCGGCTCCCAAACGGCCTCGCATGGTTCAGGTGGTGAGGGCACTAGATACTGAAAGTGACATGTCCGACCTCTCCAATGGTGTCAAATTTGGCCAGAGTACCATCTATGATTCTGGACAATACAGTCAAGACATTTCTAGATTCAGGAGAATGGCACTCGGTGGAGACAGCTCCGAATTCGACGTTTATAGTAGCGAATACAATTCAAGAGAAATGACTGCAAGTCAAGAATCATGGAGATACCAAGATAACTCAAGTGGTGAACAAGAAACTCGAGCCTTTAAAGGACAAAGT

Protein sequence

SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQIPSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS

Homology

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BLAST of MS015065 vs. NCBI nr
Match: XP_022151188.1 (LOW QUALITY PROTEIN: proline-rich receptor-like protein kinase PERK13 [Momordica charantia])

HSP 1 Score: 1033.5 bits (2671), Expect = 7.9e-298
Identity = 542/585 (92.65%), Postives = 544/585 (92.99%), Query Frame = 0


Query: 67  MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS 126
           MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS
Sbjct: 1   MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS 60

Query: 127 DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP 186
           DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP
Sbjct: 61  DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP 120

Query: 187 YMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSPEGFYTQ 246
           YMPPNNFCVKS                           DGNYYPHQHMGNSGSPEGFYTQ
Sbjct: 121 YMPPNNFCVKS---------------------------DGNYYPHQHMGNSGSPEGFYTQ 180

Query: 247 VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILGEGGFGC 306
           VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEIT GFSRQNILGEGGFGC
Sbjct: 181 VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITFGFSRQNILGEGGFGC 240

Query: 307 VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY 366
           VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY
Sbjct: 241 VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY 300

Query: 367 EFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAK-GLAYLHEDCHPKIIHRDIKSANIL 426
           EFVPNMTLEHHLH SKGVPVLDWSKRLKIAL   +     +    HPKIIHRDIKSANIL
Sbjct: 301 EFVPNMTLEHHLH-SKGVPVLDWSKRLKIALELQRVWHICMRIVSHPKIIHRDIKSANIL 360

Query: 427 LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL 486
           LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL
Sbjct: 361 LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL 420

Query: 487 ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA 546
           ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA
Sbjct: 421 ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA 480

Query: 547 AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL 606
           AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL
Sbjct: 481 AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL 540

Query: 607 GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS
Sbjct: 541 GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 557

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BLAST of MS015065 vs. NCBI nr
Match: XP_038892953.1 (proline-rich receptor-like protein kinase PERK13 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 3.2e-251
Identity = 478/650 (73.54%), Postives = 522/650 (80.31%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  E+  +   N +    P    EP  SE ++P PP  +P  IPS GA P T K+ 
Sbjct: 164 SPPSPISEDEPSIPVNQSAVQAPSTAEEPPPSEQTDPLPPTENPVAIPSPGAKPATGKRT 223

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
           PSPP  + T           P++++N  S          P T TST           TPN
Sbjct: 224 PSPPQGIPT-----------PTSESNILS----------PPTATSTR----------TPN 283

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N   S+DS P+KS  G+ + P SGSS +T VAVGAA+AGV +I+L AV+FVF+RKKKRR 
Sbjct: 284 NSPHSTDSTPVKSPLGQSNAPSSGSSRHTDVAVGAAVAGVFVIVLFAVVFVFTRKKKRR- 343

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
           ++Y GPYMPPNNFCVKS                           DGNYYP QH GNSGS 
Sbjct: 344 EMYTGPYMPPNNFCVKS---------------------------DGNYYPAQHGGNSGST 403

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTPVGNSFGSQKGT YSGSG+ESGVI++AKFFFSYEELMEITSGFSRQNILG
Sbjct: 404 EGFYTQVPHTPVGNSFGSQKGTGYSGSGMESGVINSAKFFFSYEELMEITSGFSRQNILG 463

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLPEGK VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSE+
Sbjct: 464 EGGFGCVYQGWLPEGKSVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEH 523

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIYEFVPN TLEHHLHSS GVPVLDWSKRLKIALGAAKGLAYLHEDCHP+IIHRDIK
Sbjct: 524 HRLLIYEFVPNKTLEHHLHSSNGVPVLDWSKRLKIALGAAKGLAYLHEDCHPRIIHRDIK 583

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 584 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 643

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVDP+QPLGDESLVEWARPHL+ ALETGEF  L DPRL K YVESEMF
Sbjct: 644 GVVLLELITGRKPVDPTQPLGDESLVEWARPHLLHALETGEFDGLVDPRLGKQYVESEMF 703

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVRALD ESDMSDLSNGVK+GQSTIYDSGQY+QDIS+F
Sbjct: 704 RMIEAAAACVRHSAPKRPRMVQVVRALDIESDMSDLSNGVKYGQSTIYDSGQYNQDISKF 754

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALG DS E+D+YSSEYNSREMTAS+ESWR+ +NSSGE ET+AFKG++
Sbjct: 764 RRMALGSDSFEYDMYSSEYNSREMTASRESWRFGNNSSGESETQAFKGRA 754

[Close]

BLAST of MS015065 vs. NCBI nr
Match: XP_004135247.2 (proline-rich receptor-like protein kinase PERK13 [Cucumis sativus] >KGN51804.1 hypothetical protein Csa_008034 [Cucumis sativus])

HSP 1 Score: 855.5 bits (2209), Expect = 2.9e-244
Identity = 463/650 (71.23%), Postives = 508/650 (78.15%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  E   +   + +    P+   E Q S  +NP PP  +P  IPS GA+P T KQ 
Sbjct: 174 SPPSPISEKDPSTPVDQSAIQAPNTPEETQPSTPTNPLPPSENPVVIPSPGANPATGKQT 233

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
           PS P + + T+ +  SN L P T T+                             T TPN
Sbjct: 234 PSSPDQGTITTPTSESNILSPPTATS-----------------------------TSTPN 293

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N   SSDS P+KS  G+ + P +G   +T VAVGAA+AGV +I L AV+FVFSRKKKRRG
Sbjct: 294 NSPHSSDSTPVKSPLGQSNAPSTGLRSHTDVAVGAAVAGVFVIALFAVIFVFSRKKKRRG 353

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
            +Y GPYMPP NFCVK                           ADGNYYP +H GNSGS 
Sbjct: 354 KMYTGPYMPPKNFCVK---------------------------ADGNYYPQEHGGNSGSS 413

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTP+GNSFGSQKGT YSGSG+ES VI++AKF+FSYEELME+TSGFSRQNILG
Sbjct: 414 EGFYTQVPHTPLGNSFGSQKGTGYSGSGMESSVINSAKFYFSYEELMEVTSGFSRQNILG 473

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLPEGK VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCV+E 
Sbjct: 474 EGGFGCVYQGWLPEGKTVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVAER 533

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIYEFVPN TLEHHLH  KGVPVLDWSKRLKIALG+AKGLAYLHEDCHP+IIHRDIK
Sbjct: 534 HRLLIYEFVPNKTLEHHLH-GKGVPVLDWSKRLKIALGSAKGLAYLHEDCHPRIIHRDIK 593

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAFEAQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 594 SANILLDDAFEAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 653

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVD +QPLGDESLVEWARPHL+ ALETGEF  L DPRL K YVESEMF
Sbjct: 654 GVVLLELITGRKPVDSTQPLGDESLVEWARPHLLHALETGEFDGLVDPRLGKQYVESEMF 713

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVRA+D ESDMSDLSNGVK+GQSTIYDSGQY+QDISRF
Sbjct: 714 RMIEAAAACVRHSAPKRPRMVQVVRAIDIESDMSDLSNGVKYGQSTIYDSGQYNQDISRF 766

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALG DS ++D YSSEYNS EM AS+ SWR+Q+NSSGE ET+AFKG S
Sbjct: 774 RRMALGTDSFDYDSYSSEYNSGEMNASRASWRFQNNSSGESETQAFKGGS 766

[Close]

BLAST of MS015065 vs. NCBI nr
Match: XP_022957494.1 (proline-rich receptor-like protein kinase PERK13 isoform X2 [Cucurbita moschata])

HSP 1 Score: 842.4 bits (2175), Expect = 2.6e-240
Identity = 458/650 (70.46%), Postives = 503/650 (77.38%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  EN S    N +         EP  SES++P P    PD IPS GA+    ++ 
Sbjct: 172 SPPSPISENDSPDVANESTDRGSKKAKEPPPSESTDPLPSPESPDVIPSPGANSAKGERA 231

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
            SPP E  T S +P  N   P   T+A +                             PN
Sbjct: 232 RSPPPE--TISPAPEDNIPSPPIVTSARA-----------------------------PN 291

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N  PS DS P+KS+ G+ + P S SS +T +AVGAA+ GV +I LVAV+FV  RKKKR+G
Sbjct: 292 NSPPSIDSTPVKSTLGQSNAPSSVSSSHTDIAVGAAVIGVFVIALVAVIFVLLRKKKRQG 351

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
           ++Y GPY PPNN+  KS                           DGNYYPHQHMGNSGS 
Sbjct: 352 NMYGGPYTPPNNYGAKS---------------------------DGNYYPHQHMGNSGSA 411

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTPVGNSFGSQKGT  SGSG +SGVI++AKF FSYEELMEITSGFSRQNILG
Sbjct: 412 EGFYTQVPHTPVGNSFGSQKGTGSSGSGADSGVINSAKFVFSYEELMEITSGFSRQNILG 471

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLP G+ VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN
Sbjct: 472 EGGFGCVYQGWLPAGRSVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 531

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIY+FVPN TLEHHLH   GVPVLDWSKRLKIALGAAKGLAYLHEDCHP+IIHRDIK
Sbjct: 532 HRLLIYDFVPNKTLEHHLHGGNGVPVLDWSKRLKIALGAAKGLAYLHEDCHPRIIHRDIK 591

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAF+AQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 592 SANILLDDAFDAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 651

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVDP+QPLGDESLVEWARP L+  LETG F  L DPRL K YVESEMF
Sbjct: 652 GVVLLELITGRKPVDPTQPLGDESLVEWARPLLLHTLETGVFDGLIDPRLGKQYVESEMF 711

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVR+LD E+DMSDLSNGVK+GQSTIYDSGQYSQDIS+F
Sbjct: 712 RMIEAAAACVRHSAPKRPRMVQVVRSLDIETDMSDLSNGVKYGQSTIYDSGQYSQDISKF 763

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALGGDSSEFD+YSSEY+SREM AS ESWR+++NSSGE ETRAF G++
Sbjct: 772 RRMALGGDSSEFDIYSSEYSSREMNASGESWRFENNSSGESETRAFHGRT 763

[Close]

BLAST of MS015065 vs. NCBI nr
Match: XP_022957493.1 (proline-rich receptor-like protein kinase PERK13 isoform X1 [Cucurbita moschata])

HSP 1 Score: 842.4 bits (2175), Expect = 2.6e-240
Identity = 458/650 (70.46%), Postives = 503/650 (77.38%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  EN S    N +         EP  SES++P P    PD IPS GA+    ++ 
Sbjct: 228 SPPSPISENDSPDVANESTDRGSKKAKEPPPSESTDPLPSPESPDVIPSPGANSAKGERA 287

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
            SPP E  T S +P  N   P   T+A +                             PN
Sbjct: 288 RSPPPE--TISPAPEDNIPSPPIVTSARA-----------------------------PN 347

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N  PS DS P+KS+ G+ + P S SS +T +AVGAA+ GV +I LVAV+FV  RKKKR+G
Sbjct: 348 NSPPSIDSTPVKSTLGQSNAPSSVSSSHTDIAVGAAVIGVFVIALVAVIFVLLRKKKRQG 407

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
           ++Y GPY PPNN+  KS                           DGNYYPHQHMGNSGS 
Sbjct: 408 NMYGGPYTPPNNYGAKS---------------------------DGNYYPHQHMGNSGSA 467

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTPVGNSFGSQKGT  SGSG +SGVI++AKF FSYEELMEITSGFSRQNILG
Sbjct: 468 EGFYTQVPHTPVGNSFGSQKGTGSSGSGADSGVINSAKFVFSYEELMEITSGFSRQNILG 527

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLP G+ VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN
Sbjct: 528 EGGFGCVYQGWLPAGRSVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 587

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIY+FVPN TLEHHLH   GVPVLDWSKRLKIALGAAKGLAYLHEDCHP+IIHRDIK
Sbjct: 588 HRLLIYDFVPNKTLEHHLHGGNGVPVLDWSKRLKIALGAAKGLAYLHEDCHPRIIHRDIK 647

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAF+AQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 648 SANILLDDAFDAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 707

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVDP+QPLGDESLVEWARP L+  LETG F  L DPRL K YVESEMF
Sbjct: 708 GVVLLELITGRKPVDPTQPLGDESLVEWARPLLLHTLETGVFDGLIDPRLGKQYVESEMF 767

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVR+LD E+DMSDLSNGVK+GQSTIYDSGQYSQDIS+F
Sbjct: 768 RMIEAAAACVRHSAPKRPRMVQVVRSLDIETDMSDLSNGVKYGQSTIYDSGQYSQDISKF 819

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALGGDSSEFD+YSSEY+SREM AS ESWR+++NSSGE ETRAF G++
Sbjct: 828 RRMALGGDSSEFDIYSSEYSSREMNASGESWRFENNSSGESETRAFHGRT 819

[Close]

BLAST of MS015065 vs. ExPASy Swiss-Prot
Match: Q9ZUE0 (Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702 GN=PERK12 PE=2 SV=2)

HSP 1 Score: 583.2 bits (1502), Expect = 3.7e-165
Identity = 362/717 (50.49%), Postives = 435/717 (60.67%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTS-----ESSNPTPPEGDPDTIPSTGASPK 60
           SPPSP  ++ S P  +    P PD    P  S       S P P +  P    +    P 
Sbjct: 38  SPPSPPADSSSTPPLSEPSTPPPDSQLPPLPSILPPLTDSPPPPSDSSPPVDSTPSPPPP 97

Query: 61  TTKQIPSPPGEMSTTSVSP---GSNFLPPSTDTNAD---------SDTNADAAD-ADPAT 120
           T+ + PSPP +  T    P     N  PPS D  +            TN ++     P  
Sbjct: 98  TSNESPSPPEDSETPPAPPNESNDNNPPPSQDLQSPPPSSPSPNVGPTNPESPPLQSPPA 157

Query: 121 GTSTTTTTTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPS------------------- 180
             ++  T + P   L P NP P   S P  S    P+ PPS                   
Sbjct: 158 PPASDPTNSPPASPLDPTNPPPIQPSGPATSPPANPNAPPSPFPTVPPKTPSSGPVVSPS 217

Query: 181 -------------------GSSGNTQ--VAVGAAIAGVLLIILVAVLFVFSRKKKRRGDL 240
                              G  G  Q    VG A+AG  ++ L+ V+F+  RKKKR  D 
Sbjct: 218 LTSPSKGTPTPNQGNGDGGGGGGGYQGKTMVGMAVAGFAIMALIGVVFLVRRKKKRNIDS 277

Query: 241 Y-PGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSPE 300
           Y    Y+P  NF VKS            LYG D                    G S  P 
Sbjct: 278 YNHSQYLPHPNFSVKSD---------GFLYGQD-----------------PGKGYSSGPN 337

Query: 301 GFY---TQVPHTPVGNSFGSQKG-----TVYSGSGVESGVISTAKFFFSYEELMEITSGF 360
           G     +Q   + +GNS+G+  G      + S    +S ++ + +  FSYEEL EIT GF
Sbjct: 338 GSMYNNSQQQQSSMGNSYGTAGGGYPHHQMQSSGTPDSAILGSGQTHFSYEELAEITQGF 397

Query: 361 SRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLV 420
           +R+NILGEGGFGCV+KG L +GK VAVKQLKAGSGQG+REFKAEVEIISRVHHRHLVSLV
Sbjct: 398 ARKNILGEGGFGCVYKGTLQDGKVVAVKQLKAGSGQGDREFKAEVEIISRVHHRHLVSLV 457

Query: 421 GYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPK 480
           GYC+S+ HRLLIYE+V N TLEHHLH  KG+PVL+WSKR++IA+G+AKGLAYLHEDCHPK
Sbjct: 458 GYCISDQHRLLIYEYVSNQTLEHHLH-GKGLPVLEWSKRVRIAIGSAKGLAYLHEDCHPK 517

Query: 481 IIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTD 540
           IIHRDIKSANILLDD +EAQVADFGLA+L + T THVSTRVMGTFGY+APEYASSGKLTD
Sbjct: 518 IIHRDIKSANILLDDEYEAQVADFGLARLNDTTQTHVSTRVMGTFGYLAPEYASSGKLTD 577

Query: 541 RSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKH 600
           RSDVFSFGVVLLEL+TGRKPVD +QPLG+ESLVEWARP L++A+ETG+  EL D RLEK 
Sbjct: 578 RSDVFSFGVVLLELVTGRKPVDQTQPLGEESLVEWARPLLLKAIETGDLSELIDTRLEKR 637

Query: 601 YVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQY 650
           YVE E+FRMIE AAACVRHS PKRPRMVQVVRALD + D  D+SNG+K GQST YDSGQY
Sbjct: 638 YVEHEVFRMIETAAACVRHSGPKRPRMVQVVRALDCDGDSGDISNGIKIGQSTTYDSGQY 697

[Close]

BLAST of MS015065 vs. ExPASy Swiss-Prot
Match: Q9CAL8 (Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702 GN=PERK13 PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.3e-162
Identity = 353/653 (54.06%), Postives = 414/653 (63.40%), Query Frame = 0


Query: 4   SPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIP---------STGASP 63
           SP P   S P    + +P P PD  P  S    P  P   P   P         ST + P
Sbjct: 60  SPPPTVSSPPPPPLDSSPPPPPDLTPPPSSPPPPDAPPPIPIVFPPPIDSPPPESTNSPP 119

Query: 64  KTTKQIPSPP--GEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTT 123
                 P PP   E  +    P    LPP   +              PAT     +   T
Sbjct: 120 PPEVFEPPPPPADEDESPPAPPPPEQLPPPASSPQGGPKKPKKHHPGPATSPPAPSAPAT 179

Query: 124 PTPTLTPNNPSPSSDSKPLKS---------------STGEPHTPPSGSSGNTQ--VAVGA 183
             P      P  SS + P KS               S+G    PP+ S G  Q     G 
Sbjct: 180 SPPAPPNAPPRNSSHALPPKSTAAGGPLTSPSRGVPSSGNSVPPPANSGGGYQGKTMAGF 239

Query: 184 AIAGVLLIILVAVLFVFSRKKKRRGDLY-PGPYMPPNNFCVKSGTGVIQISPLNLLYGSD 243
           AIAG  +I L+AV+F+  RKKKR  D Y    Y+PP+NF +KS            LYG  
Sbjct: 240 AIAGFAVIALMAVVFLVRRKKKRNIDAYSDSQYLPPSNFSIKSD---------GFLYG-- 299

Query: 244 VVMMLIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQK---GTVYSGSGVES 303
                            Q+     S  G Y     +  GNSFGSQ+   G   SGS  +S
Sbjct: 300 -----------------QNPTKGYSGPGGYNSQQQSNSGNSFGSQRGGGGYTRSGSAPDS 359

Query: 304 GVISTAKFFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGE 363
            V+ + +  F+YEEL +IT GFS+ NILGEGGFGCV+KG L +GK VAVKQLK GSGQG+
Sbjct: 360 AVMGSGQTHFTYEELTDITEGFSKHNILGEGGFGCVYKGKLNDGKLVAVKQLKVGSGQGD 419

Query: 364 REFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSK 423
           REFKAEVEIISRVHHRHLVSLVGYC++++ RLLIYE+VPN TLEHHLH  KG PVL+W++
Sbjct: 420 REFKAEVEIISRVHHRHLVSLVGYCIADSERLLIYEYVPNQTLEHHLH-GKGRPVLEWAR 479

Query: 424 RLKIALGAAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVS 483
           R++IA+G+AKGLAYLHEDCHPKIIHRDIKSANILLDD FEAQVADFGLAKL + T THVS
Sbjct: 480 RVRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILLDDEFEAQVADFGLAKLNDSTQTHVS 539

Query: 484 TRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARP 543
           TRVMGTFGY+APEYA SGKLTDRSDVFSFGVVLLELITGRKPVD  QPLG+ESLVEWARP
Sbjct: 540 TRVMGTFGYLAPEYAQSGKLTDRSDVFSFGVVLLELITGRKPVDQYQPLGEESLVEWARP 599

Query: 544 HLIRALETGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTES 603
            L +A+ETG+F EL D RLEKHYVE+E+FRMIE AAACVRHS PKRPRMVQVVRALD+E 
Sbjct: 600 LLHKAIETGDFSELVDRRLEKHYVENEVFRMIETAAACVRHSGPKRPRMVQVVRALDSEG 659

Query: 604 DMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMALG-GDSSEFDVYSSEYNSRE 624
           DM D+SNG K GQS+ YDSGQY+ D  +FR+MA G  DSS+  +YS +Y+ ++
Sbjct: 660 DMGDISNGNKVGQSSAYDSGQYNNDTMKFRKMAFGFDDSSDSGMYSGDYSVQD 683

[Close]

BLAST of MS015065 vs. ExPASy Swiss-Prot
Match: Q9SGY7 (Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thaliana OX=3702 GN=PERK11 PE=2 SV=2)

HSP 1 Score: 527.7 bits (1358), Expect = 1.8e-148
Identity = 334/666 (50.15%), Postives = 422/666 (63.36%), Query Frame = 0


Query: 1   SPPSPKPENRSAPT-DNGNDAPDPDPDAEPQT--------SESSNPTPPEG---DPDTIP 60
           SPP P+P  +S PT D+    P P P   P +        ++  +P P +    +P   P
Sbjct: 95  SPPQPQPPPQSTPTGDSPVVIPFPKPQLPPPSLFPPPSLVNQLPDPRPNDNNILEPINNP 154

Query: 61  STGASPKTTKQIPSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTST-- 120
            +  SP +T   P       +    P S+ LPP    N +S  N       P  G S   
Sbjct: 155 ISLPSPPSTPFSPPSQENSGSQGSPPLSSLLPPMLPLNPNSPGNPLQPLDSPLGGESNRV 214

Query: 121 -TTTTTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGN--TQVAVGAAIAGVLLI 180
            +++++   P+L+ +N      ++   +S G+  T    +  N   +  +G  IAGVL+I
Sbjct: 215 PSSSSSPSPPSLSGSNNHSGGSNRHNANSNGDGGTSQQSNESNYTEKTVIGIGIAGVLVI 274

Query: 181 ILVAVLFVFSRKKKRRGDLYP--GPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIF 240
           + +A +F F R+K+++G   P    Y+PP N  V +                        
Sbjct: 275 LFIAGVF-FVRRKQKKGSSSPRSNQYLPPANVSVNT------------------------ 334

Query: 241 LLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGV-ESGVISTAKFF 300
              +G  +  Q  GN  S     +    +P  NS G+ K     G G  +S VI T+K  
Sbjct: 335 ---EGFIHYRQKPGNGNS-----SAQNSSPDTNSLGNPK----HGRGTPDSAVIGTSKIH 394

Query: 301 FSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEI 360
           F+YEEL +IT GF +  ++GEGGFGCV+KG L EGKPVA+KQLK+ S +G REFKAEVEI
Sbjct: 395 FTYEELSQITEGFCKSFVVGEGGFGCVYKGILFEGKPVAIKQLKSVSAEGYREFKAEVEI 454

Query: 361 ISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAA 420
           ISRVHHRHLVSLVGYC+SE HR LIYEFVPN TL++HLH  K +PVL+WS+R++IA+GAA
Sbjct: 455 ISRVHHRHLVSLVGYCISEQHRFLIYEFVPNNTLDYHLH-GKNLPVLEWSRRVRIAIGAA 514

Query: 421 KGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGY 480
           KGLAYLHEDCHPKIIHRDIKS+NILLDD FEAQVADFGLA+L +   +H+STRVMGTFGY
Sbjct: 515 KGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFGLARLNDTAQSHISTRVMGTFGY 574

Query: 481 MAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETG 540
           +APEYASSGKLTDRSDVFSFGVVLLELITGRKPVD SQPLG+ESLVEWARP LI A+E G
Sbjct: 575 LAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEESLVEWARPRLIEAIEKG 634

Query: 541 EFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGV 600
           +  E+ DPRLE  YVESE+++MIE AA+CVRHSA KRPRMVQVVRALDT  D+SDL+NGV
Sbjct: 635 DISEVVDPRLENDYVESEVYKMIETAASCVRHSALKRPRMVQVVRALDTRDDLSDLTNGV 694

Query: 601 KFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGE 647
           K GQS +YDSGQYS +I  FRR +   DSS+    +  Y S++   S E          E
Sbjct: 695 KVGQSRVYDSGQYSNEIRIFRRAS--EDSSDLGTNTGYYPSQDYATSHEY---------E 711

[Close]

BLAST of MS015065 vs. ExPASy Swiss-Prot
Match: Q9FFW5 (Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 GN=PERK8 PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 4.1e-140
Identity = 325/663 (49.02%), Postives = 413/663 (62.29%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAE--PQTSESSNPTPPE-----GDPDTIPSTGAS 60
           SPP   P   S P    +  P P   A   P T  ++ P PP+       PD  PS  A 
Sbjct: 69  SPPPSPPVITSPPPTVASSPPPPVVIASPPPSTPATTPPAPPQTVSPPPPPDASPSPPA- 128

Query: 61  PKTT----KQIPSPPGEMSTTSVSPGSNFLPPS------TDTNADSDTNADAADADPATG 120
           P TT    K  PSPPGE   T   PG    PP       T T   S     A  A P + 
Sbjct: 129 PTTTNPPPKPSPSPPGE---TPSPPGETPSPPKPSPSTPTPTTTTSPPPPPATSASPPSS 188

Query: 121 TSTTTTTTTPTPTLTPNNPSPSSDSKPL-KSSTGEPHTPPSGSSGNTQVAVGAAIA-GVL 180
             T  +T  P PT  P  P     +KP   +S    +T PS S G ++V  G  +A GV+
Sbjct: 189 NPTDPSTLAPPPTPLPVVPREKPIAKPTGPASNNGNNTLPSSSPGKSEVGTGGIVAIGVI 248

Query: 181 LIILVAVLFV----FSRKKKRRG-DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVV 240
           + ++   LFV    F+RK+KR+    + G  MPP+ +           SP     GSDVV
Sbjct: 249 VGLVFLSLFVMGVWFTRKRKRKDPGTFVGYTMPPSAYS----------SP----QGSDVV 308

Query: 241 MMLIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVIST 300
           +               +  +S  P+                S  G+ Y  +  +SG++S 
Sbjct: 309 LF--------------NSRSSAPPK--------------MRSHSGSDYMYASSDSGMVSN 368

Query: 301 AKFFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKA 360
            + +FSY+EL ++TSGFS +N+LGEGGFGCV+KG L +G+ VAVKQLK G  QGEREFKA
Sbjct: 369 QRSWFSYDELSQVTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIGGSQGEREFKA 428

Query: 361 EVEIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIA 420
           EVEIISRVHHRHLV+LVGYC+SE HRLL+Y++VPN TL +HLH + G PV+ W  R+++A
Sbjct: 429 EVEIISRVHHRHLVTLVGYCISEQHRLLVYDYVPNNTLHYHLH-APGRPVMTWETRVRVA 488

Query: 421 LGAAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTN--DTHTHVSTRV 480
            GAA+G+AYLHEDCHP+IIHRDIKS+NILLD++FEA VADFGLAK+    D +THVSTRV
Sbjct: 489 AGAARGIAYLHEDCHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQELDLNTHVSTRV 548

Query: 481 MGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLI 540
           MGTFGYMAPEYA+SGKL++++DV+S+GV+LLELITGRKPVD SQPLGDESLVEWARP L 
Sbjct: 549 MGTFGYMAPEYATSGKLSEKADVYSYGVILLELITGRKPVDTSQPLGDESLVEWARPLLG 608

Query: 541 RALETGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMS 600
           +A+E  EF EL DPRL K+++  EMFRM+EAAAACVRHSA KRP+M QVVRALDT  + +
Sbjct: 609 QAIENEEFDELVDPRLGKNFIPGEMFRMVEAAAACVRHSAAKRPKMSQVVRALDTLEEAT 668

Query: 601 DLSNGVKFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQ 638
           D++NG++ GQS ++DS Q S  I  F+RMA G        YSS++  R  + S  SW  +
Sbjct: 669 DITNGMRPGQSQVFDSRQQSAQIRMFQRMAFGSQD-----YSSDFFDR--SQSHSSWGSR 677

[Close]

BLAST of MS015065 vs. ExPASy Swiss-Prot
Match: Q9SX31 (Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 GN=PERK9 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 3.9e-130
Identity = 296/635 (46.61%), Postives = 388/635 (61.10%), Query Frame = 0


Query: 3   PSPKPENRSAPTDNGNDAP-----DPDPDAEPQTSESSNPT--PPEGDPDTIPSTGASPK 62
           PS  P   S P    + +P      P P   P  S    P+  P +  P   P +  S +
Sbjct: 120 PSSPPPPASVPPPRPSPSPPILVRSPPPSVRPIQSPPPPPSDRPTQSPPPPSPPSPPSER 179

Query: 63  TTKQIPSPPGEMSTTSVSPGSNFLPPSTDTNAD-----SDTNADAADADPATGTST-TTT 122
            T+  PSPP E  T S  P S   PPS   +        DT        P +   T ++ 
Sbjct: 180 PTQSPPSPPSERPTQSPPPPSPPSPPSDRPSQSPPPPPEDTKPQPPRRSPNSPPPTFSSP 239

Query: 123 TTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVL 182
             +P   L P + +PS ++  L+     P++  +   G T   VG ++A  L++  +  +
Sbjct: 240 PRSPPEILVPGSNNPSQNNPTLRPPLDAPNSTNNSGIG-TGAVVGISVAVALVVFTLFGI 299

Query: 183 FVFSRKKKRRGDLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYY 242
           FV+  +K+ +                           L+ + G DV              
Sbjct: 300 FVWCLRKREK--------------------------RLSAVSGGDVT------------- 359

Query: 243 PHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEI 302
           P      + S   F+      PVG S   + G+  S    +SG +  +K  FSYEEL++ 
Sbjct: 360 PSPMSSTARSDSAFFRMQSSAPVGAS--KRSGSYQS----QSGGLGNSKALFSYEELVKA 419

Query: 303 TSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHL 362
           T+GFS++N+LGEGGFGCV+KG LP+G+ VAVKQLK G GQG+REFKAEVE +SR+HHRHL
Sbjct: 420 TNGFSQENLLGEGGFGCVYKGILPDGRVVAVKQLKIGGGQGDREFKAEVETLSRIHHRHL 479

Query: 363 VSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHED 422
           VS+VG+C+S + RLLIY++V N  L  HLH  K   VLDW+ R+KIA GAA+GLAYLHED
Sbjct: 480 VSIVGHCISGDRRLLIYDYVSNNDLYFHLHGEKS--VLDWATRVKIAAGAARGLAYLHED 539

Query: 423 CHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSG 482
           CHP+IIHRDIKS+NILL+D F+A+V+DFGLA+L  D +TH++TRV+GTFGYMAPEYASSG
Sbjct: 540 CHPRIIHRDIKSSNILLEDNFDARVSDFGLARLALDCNTHITTRVIGTFGYMAPEYASSG 599

Query: 483 KLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPR 542
           KLT++SDVFSFGVVLLELITGRKPVD SQPLGDESLVEWARP +  A+ET EF  L DP+
Sbjct: 600 KLTEKSDVFSFGVVLLELITGRKPVDTSQPLGDESLVEWARPLISHAIETEEFDSLADPK 659

Query: 543 LEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYD 602
           L  +YVESEMFRMIEAA ACVRH A KRPRM Q+VRA ++ +   DL+NG++ G+S +++
Sbjct: 660 LGGNYVESEMFRMIEAAGACVRHLATKRPRMGQIVRAFESLA-AEDLTNGMRLGESEVFN 705

Query: 603 SGQYSQDISRFRRMALGGDSSEFDVYS-SEYNSRE 624
           S Q S +I  FRRMA G  +   D +S S YNSR+
Sbjct: 720 SAQQSAEIRLFRRMAFGSQNYSTDFFSHSSYNSRD 705

[Close]

BLAST of MS015065 vs. ExPASy TrEMBL
Match: A0A6J1DBI2 (LOW QUALITY PROTEIN: proline-rich receptor-like protein kinase PERK13 OS=Momordica charantia OX=3673 GN=LOC111019170 PE=4 SV=1)

HSP 1 Score: 1033.5 bits (2671), Expect = 3.8e-298
Identity = 542/585 (92.65%), Postives = 544/585 (92.99%), Query Frame = 0


Query: 67  MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS 126
           MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS
Sbjct: 1   MSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPNNPSPSS 60

Query: 127 DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP 186
           DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP
Sbjct: 61  DSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRGDLYPGP 120

Query: 187 YMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSPEGFYTQ 246
           YMPPNNFCVKS                           DGNYYPHQHMGNSGSPEGFYTQ
Sbjct: 121 YMPPNNFCVKS---------------------------DGNYYPHQHMGNSGSPEGFYTQ 180

Query: 247 VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILGEGGFGC 306
           VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEIT GFSRQNILGEGGFGC
Sbjct: 181 VPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITFGFSRQNILGEGGFGC 240

Query: 307 VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY 366
           VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY
Sbjct: 241 VFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIY 300

Query: 367 EFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAK-GLAYLHEDCHPKIIHRDIKSANIL 426
           EFVPNMTLEHHLH SKGVPVLDWSKRLKIAL   +     +    HPKIIHRDIKSANIL
Sbjct: 301 EFVPNMTLEHHLH-SKGVPVLDWSKRLKIALELQRVWHICMRIVSHPKIIHRDIKSANIL 360

Query: 427 LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL 486
           LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL
Sbjct: 361 LDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLL 420

Query: 487 ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA 546
           ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA
Sbjct: 421 ELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMFRMIEA 480

Query: 547 AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL 606
           AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL
Sbjct: 481 AAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMAL 540

Query: 607 GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS
Sbjct: 541 GGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 557

[Close]

BLAST of MS015065 vs. ExPASy TrEMBL
Match: A0A0A0KSC3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G601540 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.4e-244
Identity = 463/650 (71.23%), Postives = 508/650 (78.15%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  E   +   + +    P+   E Q S  +NP PP  +P  IPS GA+P T KQ 
Sbjct: 174 SPPSPISEKDPSTPVDQSAIQAPNTPEETQPSTPTNPLPPSENPVVIPSPGANPATGKQT 233

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
           PS P + + T+ +  SN L P T T+                             T TPN
Sbjct: 234 PSSPDQGTITTPTSESNILSPPTATS-----------------------------TSTPN 293

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N   SSDS P+KS  G+ + P +G   +T VAVGAA+AGV +I L AV+FVFSRKKKRRG
Sbjct: 294 NSPHSSDSTPVKSPLGQSNAPSTGLRSHTDVAVGAAVAGVFVIALFAVIFVFSRKKKRRG 353

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
            +Y GPYMPP NFCVK                           ADGNYYP +H GNSGS 
Sbjct: 354 KMYTGPYMPPKNFCVK---------------------------ADGNYYPQEHGGNSGSS 413

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTP+GNSFGSQKGT YSGSG+ES VI++AKF+FSYEELME+TSGFSRQNILG
Sbjct: 414 EGFYTQVPHTPLGNSFGSQKGTGYSGSGMESSVINSAKFYFSYEELMEVTSGFSRQNILG 473

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLPEGK VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCV+E 
Sbjct: 474 EGGFGCVYQGWLPEGKTVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVAER 533

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIYEFVPN TLEHHLH  KGVPVLDWSKRLKIALG+AKGLAYLHEDCHP+IIHRDIK
Sbjct: 534 HRLLIYEFVPNKTLEHHLH-GKGVPVLDWSKRLKIALGSAKGLAYLHEDCHPRIIHRDIK 593

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAFEAQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 594 SANILLDDAFEAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 653

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVD +QPLGDESLVEWARPHL+ ALETGEF  L DPRL K YVESEMF
Sbjct: 654 GVVLLELITGRKPVDSTQPLGDESLVEWARPHLLHALETGEFDGLVDPRLGKQYVESEMF 713

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVRA+D ESDMSDLSNGVK+GQSTIYDSGQY+QDISRF
Sbjct: 714 RMIEAAAACVRHSAPKRPRMVQVVRAIDIESDMSDLSNGVKYGQSTIYDSGQYNQDISRF 766

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALG DS ++D YSSEYNS EM AS+ SWR+Q+NSSGE ET+AFKG S
Sbjct: 774 RRMALGTDSFDYDSYSSEYNSGEMNASRASWRFQNNSSGESETQAFKGGS 766

[Close]

BLAST of MS015065 vs. ExPASy TrEMBL
Match: A0A6J1H237 (proline-rich receptor-like protein kinase PERK13 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458874 PE=4 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 1.2e-240
Identity = 458/650 (70.46%), Postives = 503/650 (77.38%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  EN S    N +         EP  SES++P P    PD IPS GA+    ++ 
Sbjct: 172 SPPSPISENDSPDVANESTDRGSKKAKEPPPSESTDPLPSPESPDVIPSPGANSAKGERA 231

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
            SPP E  T S +P  N   P   T+A +                             PN
Sbjct: 232 RSPPPE--TISPAPEDNIPSPPIVTSARA-----------------------------PN 291

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N  PS DS P+KS+ G+ + P S SS +T +AVGAA+ GV +I LVAV+FV  RKKKR+G
Sbjct: 292 NSPPSIDSTPVKSTLGQSNAPSSVSSSHTDIAVGAAVIGVFVIALVAVIFVLLRKKKRQG 351

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
           ++Y GPY PPNN+  KS                           DGNYYPHQHMGNSGS 
Sbjct: 352 NMYGGPYTPPNNYGAKS---------------------------DGNYYPHQHMGNSGSA 411

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTPVGNSFGSQKGT  SGSG +SGVI++AKF FSYEELMEITSGFSRQNILG
Sbjct: 412 EGFYTQVPHTPVGNSFGSQKGTGSSGSGADSGVINSAKFVFSYEELMEITSGFSRQNILG 471

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLP G+ VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN
Sbjct: 472 EGGFGCVYQGWLPAGRSVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 531

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIY+FVPN TLEHHLH   GVPVLDWSKRLKIALGAAKGLAYLHEDCHP+IIHRDIK
Sbjct: 532 HRLLIYDFVPNKTLEHHLHGGNGVPVLDWSKRLKIALGAAKGLAYLHEDCHPRIIHRDIK 591

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAF+AQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 592 SANILLDDAFDAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 651

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVDP+QPLGDESLVEWARP L+  LETG F  L DPRL K YVESEMF
Sbjct: 652 GVVLLELITGRKPVDPTQPLGDESLVEWARPLLLHTLETGVFDGLIDPRLGKQYVESEMF 711

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVR+LD E+DMSDLSNGVK+GQSTIYDSGQYSQDIS+F
Sbjct: 712 RMIEAAAACVRHSAPKRPRMVQVVRSLDIETDMSDLSNGVKYGQSTIYDSGQYSQDISKF 763

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALGGDSSEFD+YSSEY+SREM AS ESWR+++NSSGE ETRAF G++
Sbjct: 772 RRMALGGDSSEFDIYSSEYSSREMNASGESWRFENNSSGESETRAFHGRT 763

[Close]

BLAST of MS015065 vs. ExPASy TrEMBL
Match: A0A6J1H0D4 (proline-rich receptor-like protein kinase PERK13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458874 PE=4 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 1.2e-240
Identity = 458/650 (70.46%), Postives = 503/650 (77.38%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIPSTGASPKTTKQI 60
           SPPSP  EN S    N +         EP  SES++P P    PD IPS GA+    ++ 
Sbjct: 228 SPPSPISENDSPDVANESTDRGSKKAKEPPPSESTDPLPSPESPDVIPSPGANSAKGERA 287

Query: 61  PSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTTPTPTLTPN 120
            SPP E  T S +P  N   P   T+A +                             PN
Sbjct: 288 RSPPPE--TISPAPEDNIPSPPIVTSARA-----------------------------PN 347

Query: 121 NPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVLFVFSRKKKRRG 180
           N  PS DS P+KS+ G+ + P S SS +T +AVGAA+ GV +I LVAV+FV  RKKKR+G
Sbjct: 348 NSPPSIDSTPVKSTLGQSNAPSSVSSSHTDIAVGAAVIGVFVIALVAVIFVLLRKKKRQG 407

Query: 181 DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSP 240
           ++Y GPY PPNN+  KS                           DGNYYPHQHMGNSGS 
Sbjct: 408 NMYGGPYTPPNNYGAKS---------------------------DGNYYPHQHMGNSGSA 467

Query: 241 EGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEITSGFSRQNILG 300
           EGFYTQVPHTPVGNSFGSQKGT  SGSG +SGVI++AKF FSYEELMEITSGFSRQNILG
Sbjct: 468 EGFYTQVPHTPVGNSFGSQKGTGSSGSGADSGVINSAKFVFSYEELMEITSGFSRQNILG 527

Query: 301 EGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 360
           EGGFGCV++GWLP G+ VAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN
Sbjct: 528 EGGFGCVYQGWLPAGRSVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLVGYCVSEN 587

Query: 361 HRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPKIIHRDIK 420
           HRLLIY+FVPN TLEHHLH   GVPVLDWSKRLKIALGAAKGLAYLHEDCHP+IIHRDIK
Sbjct: 588 HRLLIYDFVPNKTLEHHLHGGNGVPVLDWSKRLKIALGAAKGLAYLHEDCHPRIIHRDIK 647

Query: 421 SANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 480
           SANILLDDAF+AQVADFGLAKLTNDT+THVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF
Sbjct: 648 SANILLDDAFDAQVADFGLAKLTNDTNTHVSTRVMGTFGYMAPEYASSGKLTDRSDVFSF 707

Query: 481 GVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKHYVESEMF 540
           GVVLLELITGRKPVDP+QPLGDESLVEWARP L+  LETG F  L DPRL K YVESEMF
Sbjct: 708 GVVLLELITGRKPVDPTQPLGDESLVEWARPLLLHTLETGVFDGLIDPRLGKQYVESEMF 767

Query: 541 RMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQYSQDISRF 600
           RMIEAAAACVRHSAPKRPRMVQVVR+LD E+DMSDLSNGVK+GQSTIYDSGQYSQDIS+F
Sbjct: 768 RMIEAAAACVRHSAPKRPRMVQVVRSLDIETDMSDLSNGVKYGQSTIYDSGQYSQDISKF 819

Query: 601 RRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGEQETRAFKGQS 651
           RRMALGGDSSEFD+YSSEY+SREM AS ESWR+++NSSGE ETRAF G++
Sbjct: 828 RRMALGGDSSEFDIYSSEYSSREMNASGESWRFENNSSGESETRAFHGRT 819

[Close]

BLAST of MS015065 vs. ExPASy TrEMBL
Match: A0A5D3CYU9 (Protein kinase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001030 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 4.4e-238
Identity = 466/672 (69.35%), Postives = 519/672 (77.23%), Query Frame = 0


Query: 3   PSPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDT--IPSTGASP--KTTK 62
           PSP PE+ ++P  +   +P P P + P + + S   PP   P T   P+   SP  K ++
Sbjct: 115 PSPPPEDSASPPPSPPPSPRPPPPSPPPSQDPSVDPPPSPVPTTKASPAPPGSPPSKISE 174

Query: 63  QIPSPPGEMSTTSVSPGSNFLPPSTDTN----ADSDTNADAADADPATGTSTTT----TT 122
           + PS P + S           PPST T+    + +     +  A+PATG  T      T 
Sbjct: 175 KDPSTPVDQSAIQAPNTPKETPPSTPTDPLPPSQNPVVIPSPGANPATGKQTPNPPQGTI 234

Query: 123 TTP------------TPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIA 182
           TTP            T T TPNN   SSDS P+KSS G+ + P SG S +T VAVGAA+A
Sbjct: 235 TTPTSESNILSPPTATSTRTPNNSPHSSDSTPVKSSLGQSNAPSSGLSSHTDVAVGAAVA 294

Query: 183 GVLLIILVAVLFVFSRKKKRRGDLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMM 242
           GV  I L AV+F+F+RKKKRR  +Y GPYMPPNNFCVK                      
Sbjct: 295 GVFAIALFAVIFIFTRKKKRRVKMYTGPYMPPNNFCVK---------------------- 354

Query: 243 LIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAK 302
                ADGNYYP QH GNSGS EGFYTQVPHTP+GNSFGSQKGT YSGSG+ESGVI++AK
Sbjct: 355 -----ADGNYYPQQHGGNSGSTEGFYTQVPHTPLGNSFGSQKGTGYSGSGMESGVINSAK 414

Query: 303 FFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEV 362
           FFFSYEELME+TSGFSRQNILGEGGFGCV++GWLPEGK VAVKQLKAGSGQGEREFKAEV
Sbjct: 415 FFFSYEELMEVTSGFSRQNILGEGGFGCVYQGWLPEGKSVAVKQLKAGSGQGEREFKAEV 474

Query: 363 EIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALG 422
           EIISRVHHRHLVSLVGYCVSE HRLLIYEFVPN TLEHHLH + GVPVLDWSKRLKIALG
Sbjct: 475 EIISRVHHRHLVSLVGYCVSERHRLLIYEFVPNKTLEHHLHGN-GVPVLDWSKRLKIALG 534

Query: 423 AAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTF 482
           +AKGLAYLHEDCHP+IIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTF
Sbjct: 535 SAKGLAYLHEDCHPRIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTF 594

Query: 483 GYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALE 542
           GYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDP+QPLGDESLVEWARPHL+ ALE
Sbjct: 595 GYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPTQPLGDESLVEWARPHLLHALE 654

Query: 543 TGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSN 602
           TGEF  L DPRL K YVESEMFRMIEAAAACVRHSAPKRPRM+QVVRALD ESDMSDLSN
Sbjct: 655 TGEFDGLVDPRLGKQYVESEMFRMIEAAAACVRHSAPKRPRMIQVVRALDIESDMSDLSN 714

Query: 603 GVKFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSS 651
           GVK+GQST+YDSGQY+QDIS+FRRMALG DS ++D YS    S E+ AS+ESWR+Q+ SS
Sbjct: 715 GVKYGQSTMYDSGQYNQDISKFRRMALGTDSFDYDSYS----SGEINASRESWRFQNISS 754

[Close]

BLAST of MS015065 vs. TAIR 10
Match: AT1G23540.1 (Protein kinase superfamily protein )

HSP 1 Score: 583.2 bits (1502), Expect = 2.6e-166
Identity = 362/717 (50.49%), Postives = 435/717 (60.67%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAEPQTS-----ESSNPTPPEGDPDTIPSTGASPK 60
           SPPSP  ++ S P  +    P PD    P  S       S P P +  P    +    P 
Sbjct: 38  SPPSPPADSSSTPPLSEPSTPPPDSQLPPLPSILPPLTDSPPPPSDSSPPVDSTPSPPPP 97

Query: 61  TTKQIPSPPGEMSTTSVSP---GSNFLPPSTDTNAD---------SDTNADAAD-ADPAT 120
           T+ + PSPP +  T    P     N  PPS D  +            TN ++     P  
Sbjct: 98  TSNESPSPPEDSETPPAPPNESNDNNPPPSQDLQSPPPSSPSPNVGPTNPESPPLQSPPA 157

Query: 121 GTSTTTTTTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPS------------------- 180
             ++  T + P   L P NP P   S P  S    P+ PPS                   
Sbjct: 158 PPASDPTNSPPASPLDPTNPPPIQPSGPATSPPANPNAPPSPFPTVPPKTPSSGPVVSPS 217

Query: 181 -------------------GSSGNTQ--VAVGAAIAGVLLIILVAVLFVFSRKKKRRGDL 240
                              G  G  Q    VG A+AG  ++ L+ V+F+  RKKKR  D 
Sbjct: 218 LTSPSKGTPTPNQGNGDGGGGGGGYQGKTMVGMAVAGFAIMALIGVVFLVRRKKKRNIDS 277

Query: 241 Y-PGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYYPHQHMGNSGSPE 300
           Y    Y+P  NF VKS            LYG D                    G S  P 
Sbjct: 278 YNHSQYLPHPNFSVKSD---------GFLYGQD-----------------PGKGYSSGPN 337

Query: 301 GFY---TQVPHTPVGNSFGSQKG-----TVYSGSGVESGVISTAKFFFSYEELMEITSGF 360
           G     +Q   + +GNS+G+  G      + S    +S ++ + +  FSYEEL EIT GF
Sbjct: 338 GSMYNNSQQQQSSMGNSYGTAGGGYPHHQMQSSGTPDSAILGSGQTHFSYEELAEITQGF 397

Query: 361 SRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHLVSLV 420
           +R+NILGEGGFGCV+KG L +GK VAVKQLKAGSGQG+REFKAEVEIISRVHHRHLVSLV
Sbjct: 398 ARKNILGEGGFGCVYKGTLQDGKVVAVKQLKAGSGQGDREFKAEVEIISRVHHRHLVSLV 457

Query: 421 GYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHEDCHPK 480
           GYC+S+ HRLLIYE+V N TLEHHLH  KG+PVL+WSKR++IA+G+AKGLAYLHEDCHPK
Sbjct: 458 GYCISDQHRLLIYEYVSNQTLEHHLH-GKGLPVLEWSKRVRIAIGSAKGLAYLHEDCHPK 517

Query: 481 IIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSGKLTD 540
           IIHRDIKSANILLDD +EAQVADFGLA+L + T THVSTRVMGTFGY+APEYASSGKLTD
Sbjct: 518 IIHRDIKSANILLDDEYEAQVADFGLARLNDTTQTHVSTRVMGTFGYLAPEYASSGKLTD 577

Query: 541 RSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPRLEKH 600
           RSDVFSFGVVLLEL+TGRKPVD +QPLG+ESLVEWARP L++A+ETG+  EL D RLEK 
Sbjct: 578 RSDVFSFGVVLLELVTGRKPVDQTQPLGEESLVEWARPLLLKAIETGDLSELIDTRLEKR 637

Query: 601 YVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYDSGQY 650
           YVE E+FRMIE AAACVRHS PKRPRMVQVVRALD + D  D+SNG+K GQST YDSGQY
Sbjct: 638 YVEHEVFRMIETAAACVRHSGPKRPRMVQVVRALDCDGDSGDISNGIKIGQSTTYDSGQY 697

[Close]

BLAST of MS015065 vs. TAIR 10
Match: AT1G70460.1 (root hair specific 10 )

HSP 1 Score: 574.7 bits (1480), Expect = 9.3e-164
Identity = 353/653 (54.06%), Postives = 414/653 (63.40%), Query Frame = 0


Query: 4   SPKPENRSAPTDNGNDAPDPDPDAEPQTSESSNPTPPEGDPDTIP---------STGASP 63
           SP P   S P    + +P P PD  P  S    P  P   P   P         ST + P
Sbjct: 60  SPPPTVSSPPPPPLDSSPPPPPDLTPPPSSPPPPDAPPPIPIVFPPPIDSPPPESTNSPP 119

Query: 64  KTTKQIPSPP--GEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTSTTTTTTT 123
                 P PP   E  +    P    LPP   +              PAT     +   T
Sbjct: 120 PPEVFEPPPPPADEDESPPAPPPPEQLPPPASSPQGGPKKPKKHHPGPATSPPAPSAPAT 179

Query: 124 PTPTLTPNNPSPSSDSKPLKS---------------STGEPHTPPSGSSGNTQ--VAVGA 183
             P      P  SS + P KS               S+G    PP+ S G  Q     G 
Sbjct: 180 SPPAPPNAPPRNSSHALPPKSTAAGGPLTSPSRGVPSSGNSVPPPANSGGGYQGKTMAGF 239

Query: 184 AIAGVLLIILVAVLFVFSRKKKRRGDLY-PGPYMPPNNFCVKSGTGVIQISPLNLLYGSD 243
           AIAG  +I L+AV+F+  RKKKR  D Y    Y+PP+NF +KS            LYG  
Sbjct: 240 AIAGFAVIALMAVVFLVRRKKKRNIDAYSDSQYLPPSNFSIKSD---------GFLYG-- 299

Query: 244 VVMMLIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQK---GTVYSGSGVES 303
                            Q+     S  G Y     +  GNSFGSQ+   G   SGS  +S
Sbjct: 300 -----------------QNPTKGYSGPGGYNSQQQSNSGNSFGSQRGGGGYTRSGSAPDS 359

Query: 304 GVISTAKFFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGE 363
            V+ + +  F+YEEL +IT GFS+ NILGEGGFGCV+KG L +GK VAVKQLK GSGQG+
Sbjct: 360 AVMGSGQTHFTYEELTDITEGFSKHNILGEGGFGCVYKGKLNDGKLVAVKQLKVGSGQGD 419

Query: 364 REFKAEVEIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSK 423
           REFKAEVEIISRVHHRHLVSLVGYC++++ RLLIYE+VPN TLEHHLH  KG PVL+W++
Sbjct: 420 REFKAEVEIISRVHHRHLVSLVGYCIADSERLLIYEYVPNQTLEHHLH-GKGRPVLEWAR 479

Query: 424 RLKIALGAAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVS 483
           R++IA+G+AKGLAYLHEDCHPKIIHRDIKSANILLDD FEAQVADFGLAKL + T THVS
Sbjct: 480 RVRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILLDDEFEAQVADFGLAKLNDSTQTHVS 539

Query: 484 TRVMGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARP 543
           TRVMGTFGY+APEYA SGKLTDRSDVFSFGVVLLELITGRKPVD  QPLG+ESLVEWARP
Sbjct: 540 TRVMGTFGYLAPEYAQSGKLTDRSDVFSFGVVLLELITGRKPVDQYQPLGEESLVEWARP 599

Query: 544 HLIRALETGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTES 603
            L +A+ETG+F EL D RLEKHYVE+E+FRMIE AAACVRHS PKRPRMVQVVRALD+E 
Sbjct: 600 LLHKAIETGDFSELVDRRLEKHYVENEVFRMIETAAACVRHSGPKRPRMVQVVRALDSEG 659

Query: 604 DMSDLSNGVKFGQSTIYDSGQYSQDISRFRRMALG-GDSSEFDVYSSEYNSRE 624
           DM D+SNG K GQS+ YDSGQY+ D  +FR+MA G  DSS+  +YS +Y+ ++
Sbjct: 660 DMGDISNGNKVGQSSAYDSGQYNNDTMKFRKMAFGFDDSSDSGMYSGDYSVQD 683

[Close]

BLAST of MS015065 vs. TAIR 10
Match: AT1G10620.1 (Protein kinase superfamily protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.3e-149
Identity = 334/666 (50.15%), Postives = 422/666 (63.36%), Query Frame = 0


Query: 1   SPPSPKPENRSAPT-DNGNDAPDPDPDAEPQT--------SESSNPTPPEG---DPDTIP 60
           SPP P+P  +S PT D+    P P P   P +        ++  +P P +    +P   P
Sbjct: 95  SPPQPQPPPQSTPTGDSPVVIPFPKPQLPPPSLFPPPSLVNQLPDPRPNDNNILEPINNP 154

Query: 61  STGASPKTTKQIPSPPGEMSTTSVSPGSNFLPPSTDTNADSDTNADAADADPATGTST-- 120
            +  SP +T   P       +    P S+ LPP    N +S  N       P  G S   
Sbjct: 155 ISLPSPPSTPFSPPSQENSGSQGSPPLSSLLPPMLPLNPNSPGNPLQPLDSPLGGESNRV 214

Query: 121 -TTTTTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGN--TQVAVGAAIAGVLLI 180
            +++++   P+L+ +N      ++   +S G+  T    +  N   +  +G  IAGVL+I
Sbjct: 215 PSSSSSPSPPSLSGSNNHSGGSNRHNANSNGDGGTSQQSNESNYTEKTVIGIGIAGVLVI 274

Query: 181 ILVAVLFVFSRKKKRRGDLYP--GPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIF 240
           + +A +F F R+K+++G   P    Y+PP N  V +                        
Sbjct: 275 LFIAGVF-FVRRKQKKGSSSPRSNQYLPPANVSVNT------------------------ 334

Query: 241 LLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGV-ESGVISTAKFF 300
              +G  +  Q  GN  S     +    +P  NS G+ K     G G  +S VI T+K  
Sbjct: 335 ---EGFIHYRQKPGNGNS-----SAQNSSPDTNSLGNPK----HGRGTPDSAVIGTSKIH 394

Query: 301 FSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEI 360
           F+YEEL +IT GF +  ++GEGGFGCV+KG L EGKPVA+KQLK+ S +G REFKAEVEI
Sbjct: 395 FTYEELSQITEGFCKSFVVGEGGFGCVYKGILFEGKPVAIKQLKSVSAEGYREFKAEVEI 454

Query: 361 ISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAA 420
           ISRVHHRHLVSLVGYC+SE HR LIYEFVPN TL++HLH  K +PVL+WS+R++IA+GAA
Sbjct: 455 ISRVHHRHLVSLVGYCISEQHRFLIYEFVPNNTLDYHLH-GKNLPVLEWSRRVRIAIGAA 514

Query: 421 KGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGY 480
           KGLAYLHEDCHPKIIHRDIKS+NILLDD FEAQVADFGLA+L +   +H+STRVMGTFGY
Sbjct: 515 KGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFGLARLNDTAQSHISTRVMGTFGY 574

Query: 481 MAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETG 540
           +APEYASSGKLTDRSDVFSFGVVLLELITGRKPVD SQPLG+ESLVEWARP LI A+E G
Sbjct: 575 LAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEESLVEWARPRLIEAIEKG 634

Query: 541 EFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGV 600
           +  E+ DPRLE  YVESE+++MIE AA+CVRHSA KRPRMVQVVRALDT  D+SDL+NGV
Sbjct: 635 DISEVVDPRLENDYVESEVYKMIETAASCVRHSALKRPRMVQVVRALDTRDDLSDLTNGV 694

Query: 601 KFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQDNSSGE 647
           K GQS +YDSGQYS +I  FRR +   DSS+    +  Y S++   S E          E
Sbjct: 695 KVGQSRVYDSGQYSNEIRIFRRAS--EDSSDLGTNTGYYPSQDYATSHEY---------E 711

[Close]

BLAST of MS015065 vs. TAIR 10
Match: AT5G38560.1 (Protein kinase superfamily protein )

HSP 1 Score: 500.0 bits (1286), Expect = 2.9e-141
Identity = 325/663 (49.02%), Postives = 413/663 (62.29%), Query Frame = 0


Query: 1   SPPSPKPENRSAPTDNGNDAPDPDPDAE--PQTSESSNPTPPE-----GDPDTIPSTGAS 60
           SPP   P   S P    +  P P   A   P T  ++ P PP+       PD  PS  A 
Sbjct: 69  SPPPSPPVITSPPPTVASSPPPPVVIASPPPSTPATTPPAPPQTVSPPPPPDASPSPPA- 128

Query: 61  PKTT----KQIPSPPGEMSTTSVSPGSNFLPPS------TDTNADSDTNADAADADPATG 120
           P TT    K  PSPPGE   T   PG    PP       T T   S     A  A P + 
Sbjct: 129 PTTTNPPPKPSPSPPGE---TPSPPGETPSPPKPSPSTPTPTTTTSPPPPPATSASPPSS 188

Query: 121 TSTTTTTTTPTPTLTPNNPSPSSDSKPL-KSSTGEPHTPPSGSSGNTQVAVGAAIA-GVL 180
             T  +T  P PT  P  P     +KP   +S    +T PS S G ++V  G  +A GV+
Sbjct: 189 NPTDPSTLAPPPTPLPVVPREKPIAKPTGPASNNGNNTLPSSSPGKSEVGTGGIVAIGVI 248

Query: 181 LIILVAVLFV----FSRKKKRRG-DLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVV 240
           + ++   LFV    F+RK+KR+    + G  MPP+ +           SP     GSDVV
Sbjct: 249 VGLVFLSLFVMGVWFTRKRKRKDPGTFVGYTMPPSAYS----------SP----QGSDVV 308

Query: 241 MMLIFLLADGNYYPHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVIST 300
           +               +  +S  P+                S  G+ Y  +  +SG++S 
Sbjct: 309 LF--------------NSRSSAPPK--------------MRSHSGSDYMYASSDSGMVSN 368

Query: 301 AKFFFSYEELMEITSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKA 360
            + +FSY+EL ++TSGFS +N+LGEGGFGCV+KG L +G+ VAVKQLK G  QGEREFKA
Sbjct: 369 QRSWFSYDELSQVTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIGGSQGEREFKA 428

Query: 361 EVEIISRVHHRHLVSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIA 420
           EVEIISRVHHRHLV+LVGYC+SE HRLL+Y++VPN TL +HLH + G PV+ W  R+++A
Sbjct: 429 EVEIISRVHHRHLVTLVGYCISEQHRLLVYDYVPNNTLHYHLH-APGRPVMTWETRVRVA 488

Query: 421 LGAAKGLAYLHEDCHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTN--DTHTHVSTRV 480
            GAA+G+AYLHEDCHP+IIHRDIKS+NILLD++FEA VADFGLAK+    D +THVSTRV
Sbjct: 489 AGAARGIAYLHEDCHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQELDLNTHVSTRV 548

Query: 481 MGTFGYMAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLI 540
           MGTFGYMAPEYA+SGKL++++DV+S+GV+LLELITGRKPVD SQPLGDESLVEWARP L 
Sbjct: 549 MGTFGYMAPEYATSGKLSEKADVYSYGVILLELITGRKPVDTSQPLGDESLVEWARPLLG 608

Query: 541 RALETGEFGELTDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMS 600
           +A+E  EF EL DPRL K+++  EMFRM+EAAAACVRHSA KRP+M QVVRALDT  + +
Sbjct: 609 QAIENEEFDELVDPRLGKNFIPGEMFRMVEAAAACVRHSAAKRPKMSQVVRALDTLEEAT 668

Query: 601 DLSNGVKFGQSTIYDSGQYSQDISRFRRMALGGDSSEFDVYSSEYNSREMTASQESWRYQ 638
           D++NG++ GQS ++DS Q S  I  F+RMA G        YSS++  R  + S  SW  +
Sbjct: 669 DITNGMRPGQSQVFDSRQQSAQIRMFQRMAFGSQD-----YSSDFFDR--SQSHSSWGSR 677

[Close]

BLAST of MS015065 vs. TAIR 10
Match: AT1G68690.1 (Protein kinase superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 2.7e-131
Identity = 296/635 (46.61%), Postives = 388/635 (61.10%), Query Frame = 0


Query: 3   PSPKPENRSAPTDNGNDAP-----DPDPDAEPQTSESSNPT--PPEGDPDTIPSTGASPK 62
           PS  P   S P    + +P      P P   P  S    P+  P +  P   P +  S +
Sbjct: 120 PSSPPPPASVPPPRPSPSPPILVRSPPPSVRPIQSPPPPPSDRPTQSPPPPSPPSPPSER 179

Query: 63  TTKQIPSPPGEMSTTSVSPGSNFLPPSTDTNAD-----SDTNADAADADPATGTST-TTT 122
            T+  PSPP E  T S  P S   PPS   +        DT        P +   T ++ 
Sbjct: 180 PTQSPPSPPSERPTQSPPPPSPPSPPSDRPSQSPPPPPEDTKPQPPRRSPNSPPPTFSSP 239

Query: 123 TTTPTPTLTPNNPSPSSDSKPLKSSTGEPHTPPSGSSGNTQVAVGAAIAGVLLIILVAVL 182
             +P   L P + +PS ++  L+     P++  +   G T   VG ++A  L++  +  +
Sbjct: 240 PRSPPEILVPGSNNPSQNNPTLRPPLDAPNSTNNSGIG-TGAVVGISVAVALVVFTLFGI 299

Query: 183 FVFSRKKKRRGDLYPGPYMPPNNFCVKSGTGVIQISPLNLLYGSDVVMMLIFLLADGNYY 242
           FV+  +K+ +                           L+ + G DV              
Sbjct: 300 FVWCLRKREK--------------------------RLSAVSGGDVT------------- 359

Query: 243 PHQHMGNSGSPEGFYTQVPHTPVGNSFGSQKGTVYSGSGVESGVISTAKFFFSYEELMEI 302
           P      + S   F+      PVG S   + G+  S    +SG +  +K  FSYEEL++ 
Sbjct: 360 PSPMSSTARSDSAFFRMQSSAPVGAS--KRSGSYQS----QSGGLGNSKALFSYEELVKA 419

Query: 303 TSGFSRQNILGEGGFGCVFKGWLPEGKPVAVKQLKAGSGQGEREFKAEVEIISRVHHRHL 362
           T+GFS++N+LGEGGFGCV+KG LP+G+ VAVKQLK G GQG+REFKAEVE +SR+HHRHL
Sbjct: 420 TNGFSQENLLGEGGFGCVYKGILPDGRVVAVKQLKIGGGQGDREFKAEVETLSRIHHRHL 479

Query: 363 VSLVGYCVSENHRLLIYEFVPNMTLEHHLHSSKGVPVLDWSKRLKIALGAAKGLAYLHED 422
           VS+VG+C+S + RLLIY++V N  L  HLH  K   VLDW+ R+KIA GAA+GLAYLHED
Sbjct: 480 VSIVGHCISGDRRLLIYDYVSNNDLYFHLHGEKS--VLDWATRVKIAAGAARGLAYLHED 539

Query: 423 CHPKIIHRDIKSANILLDDAFEAQVADFGLAKLTNDTHTHVSTRVMGTFGYMAPEYASSG 482
           CHP+IIHRDIKS+NILL+D F+A+V+DFGLA+L  D +TH++TRV+GTFGYMAPEYASSG
Sbjct: 540 CHPRIIHRDIKSSNILLEDNFDARVSDFGLARLALDCNTHITTRVIGTFGYMAPEYASSG 599

Query: 483 KLTDRSDVFSFGVVLLELITGRKPVDPSQPLGDESLVEWARPHLIRALETGEFGELTDPR 542
           KLT++SDVFSFGVVLLELITGRKPVD SQPLGDESLVEWARP +  A+ET EF  L DP+
Sbjct: 600 KLTEKSDVFSFGVVLLELITGRKPVDTSQPLGDESLVEWARPLISHAIETEEFDSLADPK 659

Query: 543 LEKHYVESEMFRMIEAAAACVRHSAPKRPRMVQVVRALDTESDMSDLSNGVKFGQSTIYD 602
           L  +YVESEMFRMIEAA ACVRH A KRPRM Q+VRA ++ +   DL+NG++ G+S +++
Sbjct: 660 LGGNYVESEMFRMIEAAGACVRHLATKRPRMGQIVRAFESLA-AEDLTNGMRLGESEVFN 705

Query: 603 SGQYSQDISRFRRMALGGDSSEFDVYS-SEYNSRE 624
           S Q S +I  FRRMA G  +   D +S S YNSR+
Sbjct: 720 SAQQSAEIRLFRRMAFGSQNYSTDFFSHSSYNSRD 705

The following BLAST results are available for this feature:

BLAST of MS015065 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151188.1	7.9e-298	92.65	LOW QUALITY PROTEIN: proline-rich receptor-like protein kinase PERK13 [Momordica...	[more]
XP_038892953.1	3.2e-251	73.54	proline-rich receptor-like protein kinase PERK13 [Benincasa hispida]	[more]
XP_004135247.2	2.9e-244	71.23	proline-rich receptor-like protein kinase PERK13 [Cucumis sativus] >KGN51804.1 h...	[more]
XP_022957494.1	2.6e-240	70.46	proline-rich receptor-like protein kinase PERK13 isoform X2 [Cucurbita moschata]	[more]
XP_022957493.1	2.6e-240	70.46	proline-rich receptor-like protein kinase PERK13 isoform X1 [Cucurbita moschata]	[more]

BLAST of MS015065 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUE0	3.7e-165	50.49	Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702...	[more]
Q9CAL8	1.3e-162	54.06	Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702...	[more]
Q9SGY7	1.8e-148	50.15	Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thalian...	[more]
Q9FFW5	4.1e-140	49.02	Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SX31	3.9e-130	46.61	Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MS015065 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBI2	3.8e-298	92.65	LOW QUALITY PROTEIN: proline-rich receptor-like protein kinase PERK13 OS=Momordi...	[more]
A0A0A0KSC3	1.4e-244	71.23	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G601...	[more]
A0A6J1H237	1.2e-240	70.46	proline-rich receptor-like protein kinase PERK13 isoform X2 OS=Cucurbita moschat...	[more]
A0A6J1H0D4	1.2e-240	70.46	proline-rich receptor-like protein kinase PERK13 isoform X1 OS=Cucurbita moschat...	[more]
A0A5D3CYU9	4.4e-238	69.35	Protein kinase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of MS015065 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23540.1	2.6e-166	50.49	Protein kinase superfamily protein	[more]
AT1G70460.1	9.3e-164	54.06	root hair specific 10	[more]
AT1G10620.1	1.3e-149	50.15	Protein kinase superfamily protein	[more]
AT5G38560.1	2.9e-141	49.02	Protein kinase superfamily protein	[more]
AT1G68690.1	2.7e-131	46.61	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 293..567 e-value: 7.2E-34 score: 128.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 293..573 score: 37.389389
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 259..368 e-value: 3.9E-35 score: 122.0
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 369..584 e-value: 1.1E-62 score: 213.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..148
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 625..650
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..148
None	No IPR available	PANTHER	PTHR47982:SF44	PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK13-RELATED	coord: 2..634
None	No IPR available	PANTHER	PTHR47982	PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4	coord: 2..634
None	No IPR available	CDD	cd14066	STKc_IRAK	coord: 299..568 e-value: 3.77662E-107 score: 323.07
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 296..566 e-value: 5.9E-45 score: 153.6
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 414..426
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 299..321
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 273..567

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015065.1	MS015065.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity