MS015042 (gene) Bitter gourd (TR) v1

Overview
NameMS015042
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionpectinesterase inhibitor-like
Locationscaffold2: 795169 .. 795654 (-)
RNA-Seq ExpressionMS015042
SyntenyMS015042
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGAAAAGAATCTGGTTTGTTTGTCACTGGTCTTTCTCGCGGCGGCCGTGGCTGTCTTGGCCGGCCACGCCCACGGCGCCGACATCTGCGCGAAAGCAGATTACGTGCCCCTCTGCCGGTCCATGGCGAAGGGCGCCGCCAATAATCCGGCAGCCGCCTTAAAGGTAGCCATGGAAAAGATGATCGTCGAAACCAAACGCGCCAAAGCAGCGAGCGCCAAAATAGGCAAATCCCCGGCACTGTTGCTGTGCGCGGAGAACTATGGCTACAACATTTCGTACCTGCAGGAATGCCTGATGCTTCTGAAGATCAAGGACAAAAACACGCTGATGATCCGGCTGTCCGCGGTTGTGGCGACATACGGCGTGTGCGACGACGCCATCGGCGAGAGCGGCATCGTCACGCAAGCTGCGAGCTTGGTGTCAATCGATAAAAGGCTGACGCAGATGACTAGCAATTGCTTGCACTTCACCAGCTTGACT

mRNA sequence

ATGGGTGAAAAGAATCTGGTTTGTTTGTCACTGGTCTTTCTCGCGGCGGCCGTGGCTGTCTTGGCCGGCCACGCCCACGGCGCCGACATCTGCGCGAAAGCAGATTACGTGCCCCTCTGCCGGTCCATGGCGAAGGGCGCCGCCAATAATCCGGCAGCCGCCTTAAAGGTAGCCATGGAAAAGATGATCGTCGAAACCAAACGCGCCAAAGCAGCGAGCGCCAAAATAGGCAAATCCCCGGCACTGTTGCTGTGCGCGGAGAACTATGGCTACAACATTTCGTACCTGCAGGAATGCCTGATGCTTCTGAAGATCAAGGACAAAAACACGCTGATGATCCGGCTGTCCGCGGTTGTGGCGACATACGGCGTGTGCGACGACGCCATCGGCGAGAGCGGCATCGTCACGCAAGCTGCGAGCTTGGTGTCAATCGATAAAAGGCTGACGCAGATGACTAGCAATTGCTTGCACTTCACCAGCTTGACT

Coding sequence (CDS)

ATGGGTGAAAAGAATCTGGTTTGTTTGTCACTGGTCTTTCTCGCGGCGGCCGTGGCTGTCTTGGCCGGCCACGCCCACGGCGCCGACATCTGCGCGAAAGCAGATTACGTGCCCCTCTGCCGGTCCATGGCGAAGGGCGCCGCCAATAATCCGGCAGCCGCCTTAAAGGTAGCCATGGAAAAGATGATCGTCGAAACCAAACGCGCCAAAGCAGCGAGCGCCAAAATAGGCAAATCCCCGGCACTGTTGCTGTGCGCGGAGAACTATGGCTACAACATTTCGTACCTGCAGGAATGCCTGATGCTTCTGAAGATCAAGGACAAAAACACGCTGATGATCCGGCTGTCCGCGGTTGTGGCGACATACGGCGTGTGCGACGACGCCATCGGCGAGAGCGGCATCGTCACGCAAGCTGCGAGCTTGGTGTCAATCGATAAAAGGCTGACGCAGATGACTAGCAATTGCTTGCACTTCACCAGCTTGACT

Protein sequence

MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAMEKMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVATYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT
Homology
BLAST of MS015042 vs. NCBI nr
Match: XP_022151771.1 (uncharacterized protein LOC111019675 [Momordica charantia])

HSP 1 Score: 303.9 bits (777), Expect = 8.2e-79
Identity = 161/162 (99.38%), Postives = 161/162 (99.38%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPA ALKVAME
Sbjct: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAVALKVAME 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA
Sbjct: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
           TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT
Sbjct: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 162

BLAST of MS015042 vs. NCBI nr
Match: XP_022151947.1 (uncharacterized protein LOC111019796 [Momordica charantia])

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-33
Identity = 85/145 (58.62%), Postives = 105/145 (72.41%), Query Frame = 0

Query: 18  VAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAMEKMIVETKRAKAASAKIG 77
           VA +AGHAH ++IC KAD VPLCRSM KG A +P AA K +++++I ETKRAK AS ++G
Sbjct: 9   VAFMAGHAHVSNICGKADDVPLCRSMVKG-ATDPVAATKASIDQLITETKRAKEASVQLG 68

Query: 78  KSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVATYGVCDDAIGESGIVTQ 137
           KS  +  C +N+G NI  LQE L  LK +DK TLM  LSAVV+ Y  CDDAIGE GIVTQ
Sbjct: 69  KSSFMGACTQNFGDNILNLQEGLQALKSRDKGTLMSSLSAVVSLYSGCDDAIGEGGIVTQ 128

Query: 138 AASLVSIDKRLTQMTSNCLHFTSLT 163
           AA +VSI+ +L  M SNCL+   LT
Sbjct: 129 AAGIVSINSQLIHMASNCLYLADLT 152

BLAST of MS015042 vs. NCBI nr
Match: XP_022151923.1 (uncharacterized protein LOC111019778 [Momordica charantia])

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-32
Identity = 87/162 (53.70%), Postives = 108/162 (66.67%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MG KNLVCLSLVFLAAA+A      H ADICAKAD+V LCRSMAKG  NNP AA +  +E
Sbjct: 1   MGAKNLVCLSLVFLAAAMA----QTHAADICAKADHVALCRSMAKGVGNNPVAATRAGIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           K+I  TK A+ AS K G+S AL +C E Y   IS L+  L  L+ KDK++L I LS  + 
Sbjct: 61  KLITATKLAREASIKSGRSGALGVCKETYANAISELKTGLDYLQKKDKDSLNIELSGAMT 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
            Y  CDDAI ESG+V +A  ++  D+ L +M S CL+  +LT
Sbjct: 121 DYTTCDDAIAESGVVGKAVGVLKNDRMLLRMASTCLYLATLT 158

BLAST of MS015042 vs. NCBI nr
Match: XP_022151772.1 (uncharacterized protein LOC111019676 [Momordica charantia])

HSP 1 Score: 150.2 bits (378), Expect = 1.5e-32
Identity = 87/162 (53.70%), Postives = 107/162 (66.05%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MG KNLVCLSLV +A AVA     AH ADICAKADY  LCRSM KGA NNP AA +  +E
Sbjct: 1   MGAKNLVCLSLVLVAVAVA----QAHAADICAKADYAALCRSMVKGAGNNPVAATRAGIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           K+I+ TK A+ A  K  KS AL +C E +   IS L+  L  L+ KDK++L I LS  + 
Sbjct: 61  KLILATKIAREARLKSAKSGALGVCKETFANAISELKMGLDYLQKKDKDSLNIELSGAMT 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
            Y  CDDAI ESG+V +A  +V ID+ L  M SNCL+ ++LT
Sbjct: 121 DYTTCDDAIAESGVVGKAVGIVKIDRTLLNMASNCLYLSTLT 158

BLAST of MS015042 vs. NCBI nr
Match: XP_022151919.1 (uncharacterized protein LOC111019774 [Momordica charantia])

HSP 1 Score: 136.3 bits (342), Expect = 2.3e-28
Identity = 80/162 (49.38%), Postives = 107/162 (66.05%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MGEKNLVC SLVFLAAAVA+LAGHAHG+DIC  A+ VP CR++ KG A +P AAL+ A+E
Sbjct: 1   MGEKNLVCFSLVFLAAAVALLAGHAHGSDICVNANDVPQCRAIVKG-ATDPVAALRAAIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALL-LCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVV 120
           ++I ETKRA  AS  +  +P  L +C +N+   I  LQ  L  LKI+DK++L   LSA +
Sbjct: 61  QLIFETKRANEASVALAANPGPLDVCKQNFDNAIRDLQRSLESLKIQDKDSLESHLSAAL 120

Query: 121 ATYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSL 162
           + Y  CD A+ E  ++ Q+  +   D  L  M SN ++  SL
Sbjct: 121 SDYVRCDGAVIEGDMMVQSVGIFKTDNVLLNMASNVIYLASL 161

BLAST of MS015042 vs. ExPASy TrEMBL
Match: A0A6J1DFN6 (uncharacterized protein LOC111019675 OS=Momordica charantia OX=3673 GN=LOC111019675 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.0e-79
Identity = 161/162 (99.38%), Postives = 161/162 (99.38%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPA ALKVAME
Sbjct: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAVALKVAME 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA
Sbjct: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
           TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT
Sbjct: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 162

BLAST of MS015042 vs. ExPASy TrEMBL
Match: A0A6J1DEV2 (uncharacterized protein LOC111019796 OS=Momordica charantia OX=3673 GN=LOC111019796 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.7e-34
Identity = 85/145 (58.62%), Postives = 105/145 (72.41%), Query Frame = 0

Query: 18  VAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAMEKMIVETKRAKAASAKIG 77
           VA +AGHAH ++IC KAD VPLCRSM KG A +P AA K +++++I ETKRAK AS ++G
Sbjct: 9   VAFMAGHAHVSNICGKADDVPLCRSMVKG-ATDPVAATKASIDQLITETKRAKEASVQLG 68

Query: 78  KSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVATYGVCDDAIGESGIVTQ 137
           KS  +  C +N+G NI  LQE L  LK +DK TLM  LSAVV+ Y  CDDAIGE GIVTQ
Sbjct: 69  KSSFMGACTQNFGDNILNLQEGLQALKSRDKGTLMSSLSAVVSLYSGCDDAIGEGGIVTQ 128

Query: 138 AASLVSIDKRLTQMTSNCLHFTSLT 163
           AA +VSI+ +L  M SNCL+   LT
Sbjct: 129 AAGIVSINSQLIHMASNCLYLADLT 152

BLAST of MS015042 vs. ExPASy TrEMBL
Match: A0A6J1DDH2 (uncharacterized protein LOC111019778 OS=Momordica charantia OX=3673 GN=LOC111019778 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 5.6e-33
Identity = 87/162 (53.70%), Postives = 108/162 (66.67%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MG KNLVCLSLVFLAAA+A      H ADICAKAD+V LCRSMAKG  NNP AA +  +E
Sbjct: 1   MGAKNLVCLSLVFLAAAMA----QTHAADICAKADHVALCRSMAKGVGNNPVAATRAGIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           K+I  TK A+ AS K G+S AL +C E Y   IS L+  L  L+ KDK++L I LS  + 
Sbjct: 61  KLITATKLAREASIKSGRSGALGVCKETYANAISELKTGLDYLQKKDKDSLNIELSGAMT 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
            Y  CDDAI ESG+V +A  ++  D+ L +M S CL+  +LT
Sbjct: 121 DYTTCDDAIAESGVVGKAVGVLKNDRMLLRMASTCLYLATLT 158

BLAST of MS015042 vs. ExPASy TrEMBL
Match: A0A6J1DED8 (uncharacterized protein LOC111019676 OS=Momordica charantia OX=3673 GN=LOC111019676 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 7.4e-33
Identity = 87/162 (53.70%), Postives = 107/162 (66.05%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MG KNLVCLSLV +A AVA     AH ADICAKADY  LCRSM KGA NNP AA +  +E
Sbjct: 1   MGAKNLVCLSLVLVAVAVA----QAHAADICAKADYAALCRSMVKGAGNNPVAATRAGIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVA 120
           K+I+ TK A+ A  K  KS AL +C E +   IS L+  L  L+ KDK++L I LS  + 
Sbjct: 61  KLILATKIAREARLKSAKSGALGVCKETFANAISELKMGLDYLQKKDKDSLNIELSGAMT 120

Query: 121 TYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSLT 163
            Y  CDDAI ESG+V +A  +V ID+ L  M SNCL+ ++LT
Sbjct: 121 DYTTCDDAIAESGVVGKAVGIVKIDRTLLNMASNCLYLSTLT 158

BLAST of MS015042 vs. ExPASy TrEMBL
Match: A0A6J1DEH0 (uncharacterized protein LOC111019774 OS=Momordica charantia OX=3673 GN=LOC111019774 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.1e-28
Identity = 80/162 (49.38%), Postives = 107/162 (66.05%), Query Frame = 0

Query: 1   MGEKNLVCLSLVFLAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAME 60
           MGEKNLVC SLVFLAAAVA+LAGHAHG+DIC  A+ VP CR++ KG A +P AAL+ A+E
Sbjct: 1   MGEKNLVCFSLVFLAAAVALLAGHAHGSDICVNANDVPQCRAIVKG-ATDPVAALRAAIE 60

Query: 61  KMIVETKRAKAASAKIGKSPALL-LCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVV 120
           ++I ETKRA  AS  +  +P  L +C +N+   I  LQ  L  LKI+DK++L   LSA +
Sbjct: 61  QLIFETKRANEASVALAANPGPLDVCKQNFDNAIRDLQRSLESLKIQDKDSLESHLSAAL 120

Query: 121 ATYGVCDDAIGESGIVTQAASLVSIDKRLTQMTSNCLHFTSL 162
           + Y  CD A+ E  ++ Q+  +   D  L  M SN ++  SL
Sbjct: 121 SDYVRCDGAVIEGDMMVQSVGIFKTDNVLLNMASNVIYLASL 161

BLAST of MS015042 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 3.6e-08
Identity = 50/150 (33.33%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 14  LAAAVAVLAGHAHGADICAKADYVPLCRSMAKGAANNPAAALKVAMEKMIVETKRAKAAS 73
           +A A  V+ G   G D C+ +D+  LCRS+ KG   N  AA +V++ +++  T +AK A 
Sbjct: 23  VAMAQRVMGG---GRDPCSVSDFKVLCRSVVKG-QKNVNAATEVSIRELMKRTIKAKEA- 82

Query: 74  AKIGKSP--ALLLCAENYGYNISYLQECLMLLKIKDKNTLMIRLSAVVATYGVCDDAIGE 133
           AKI +     L  C  NY   +  LQ+ L  +K  D  +L I LSA +  +  C+DA+G 
Sbjct: 83  AKISRKSGGGLKTCYSNYDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGG 142

Query: 134 SGIVTQAASLVSIDKRLTQMTSNCLHFTSL 162
               T +         L +M  NCL  ++L
Sbjct: 143 G---TASNVFAKSTSTLHEMADNCLALSTL 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151771.18.2e-7999.38uncharacterized protein LOC111019675 [Momordica charantia][more]
XP_022151947.11.4e-3358.62uncharacterized protein LOC111019796 [Momordica charantia][more]
XP_022151923.11.2e-3253.70uncharacterized protein LOC111019778 [Momordica charantia][more]
XP_022151772.11.5e-3253.70uncharacterized protein LOC111019676 [Momordica charantia][more]
XP_022151919.12.3e-2849.38uncharacterized protein LOC111019774 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DFN64.0e-7999.38uncharacterized protein LOC111019675 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1DEV26.7e-3458.62uncharacterized protein LOC111019796 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1DDH25.6e-3353.70uncharacterized protein LOC111019778 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1DED87.4e-3353.70uncharacterized protein LOC111019676 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1DEH01.1e-2849.38uncharacterized protein LOC111019774 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
Match NameE-valueIdentityDescription
AT1G70540.13.6e-0833.33Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 22..158
e-value: 3.6E-9
score: 46.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..156
e-value: 3.8E-12
score: 46.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..162
e-value: 3.5E-13
score: 51.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 29..160
NoneNo IPR availableCDDcd15800PMEI-like_2coord: 28..157
e-value: 5.27852E-29
score: 102.053

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015042.1MS015042.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity