MS015004 (gene) Bitter gourd (TR) v1

BLAST of MS015004 vs. NCBI nr
Match: XP_022151748.1 (E3 ubiquitin-protein ligase RGLG2-like [Momordica charantia] >XP_022151751.1 E3 ubiquitin-protein ligase RGLG2-like [Momordica charantia])

HSP 1 Score: 884.8 bits (2285), Expect = 3.0e-253
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD
Sbjct: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ
Sbjct: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE
Sbjct: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT
Sbjct: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR
Sbjct: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF
Sbjct: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI
Sbjct: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRSFIQTRIKLY
Sbjct: 421 CRSFIQTRIKLY 432

BLAST of MS015004 vs. NCBI nr
Match: XP_008446129.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >XP_008446130.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >KAA0034249.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa] >TYK15671.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa])

HSP 1 Score: 756.5 bits (1952), Expect = 1.2e-214
Identity = 375/435 (86.21%), Postives = 396/435 (91.03%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPP--PSPYPQQYPYTPSSDYGSQTP-QPQR 60
           MGG+NS+E  +REF SYG  NS+ WDQ++YPP  PSPY QQYPYT  S YGSQTP QP+R
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGYGSQTPQQPRR 60

Query: 61  RLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDRKYSRIADNY SLDEVT+ALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHR 180
           N  NPYEQAISIIGQTLS FDEDNLIPCFGFGDASTHDQDVFSFY  DR CNGFEEVLHR
Sbjct: 121 NEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVLHR 180

Query: 181 YREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQE 240
           YRE+VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDTH+GQLS QE
Sbjct: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSLQE 240

Query: 241 RKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNP 300
           RKTIDAIV+ASEYPLSII VGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIM KNVN 
Sbjct: 241 RKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMLKNVNN 300

Query: 301 SRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHS 360
            RRQTEFALAALMEIPSQY+ATLELG+LGRQRGSSPERVPLPPPLY A  S+GY RT  S
Sbjct: 301 VRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSSGYPRTPCS 360

Query: 361 NSFQSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLEL 420
           NSFQ+RTP+  R N+GVGT PSSSS YDNQLCPIC+TNPKDMAFGCGH TCCDCGENLEL
Sbjct: 361 NSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGHMTCCDCGENLEL 420

Query: 421 CPICRSFIQTRIKLY 433
           CPICRSFIQTRI+LY
Sbjct: 421 CPICRSFIQTRIRLY 435

BLAST of MS015004 vs. NCBI nr
Match: XP_038892155.1 (E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida] >XP_038892156.1 E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida])

HSP 1 Score: 753.4 bits (1944), Expect = 1.0e-213
Identity = 368/432 (85.19%), Postives = 396/432 (91.67%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MGG+NS+E  +RE  SYG  NS+ WDQ+++PPPSP+  QYPY   SDYGSQTPQPQRRLD
Sbjct: 1   MGGKNSREGGQRELYSYGSGNSSTWDQYNFPPPSPHVHQYPYAEPSDYGSQTPQPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIADNY SLDEVT+ALA+AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ
Sbjct: 61  RKYSRIADNYQSLDEVTAALAKAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDNLIPCFGFGDASTHDQDVFSFYE DR CNGFEEVLHRYRE
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYEDDRICNGFEEVLHRYRE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           +VPNL+LAGPTSFAPI+EMA TIVE++GGQYHVLLIIADGQVTRSVDTH+GQLS QE+K+
Sbjct: 181 IVPNLKLAGPTSFAPIIEMAMTIVEQSGGQYHVLLIIADGQVTRSVDTHNGQLSLQEQKS 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           IDAIV+ASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTE+MSKNVN  RR
Sbjct: 241 IDAIVRASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEMMSKNVNNVRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEF+LAALMEIPSQY ATLELGLLGRQRG SPERVPLPPP+Y     NGYSRT  SN+F
Sbjct: 301 QTEFSLAALMEIPSQYLATLELGLLGRQRGISPERVPLPPPVY-----NGYSRTPRSNNF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
            +RTP+  R N+GVGT  SSSSHYDNQLCPICLTNPKD+AFGCGH TCCDCGENLELCPI
Sbjct: 361 PTRTPSLGRYNQGVGTTSSSSSHYDNQLCPICLTNPKDLAFGCGHLTCCDCGENLELCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRSFIQTRI+LY
Sbjct: 421 CRSFIQTRIRLY 427

BLAST of MS015004 vs. NCBI nr
Match: KAG6573681.1 (E3 ubiquitin-protein ligase RGLG2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 752.3 bits (1941), Expect = 2.3e-213
Identity = 364/432 (84.26%), Postives = 394/432 (91.20%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MG RNSKE  +REFDS G+ NS+ W   +YPPPSPY Q+Y Y   SDYGSQTP PQRRLD
Sbjct: 1   MGSRNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIAD+Y SLDEVT+ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDN+IPC+GFGDASTHDQ+VFSFY++DR CNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           +VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDT++GQLS QER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           +DAIV+ASEYPLSIILVGVGDGPWDMMK FDDNIPARAFDNFQFVNFTEIMSKNVN +RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQY+ATLELGLLGR+RGSSPERVPLPPPLY   P NGY RT  +NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNGYPRTPRANSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           Q+RTP+H R N+GVGT  S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGE LE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRS IQTRI+LY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of MS015004 vs. NCBI nr
Match: XP_022966892.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita maxima])

HSP 1 Score: 751.9 bits (1940), Expect = 3.0e-213
Identity = 364/432 (84.26%), Postives = 394/432 (91.20%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MG +NSKE  +REFDS G+ NS+ W   +YPPPSPY QQY Y   SDYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIAD+Y SLDEVT+ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDN+IPC+GFGDASTHDQ+VFSFY++DR CNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           +VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDT++GQLS QER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           +DAIV+ASEYPLSIILVGVGDGPWDMMK FDDNIPARAFDNFQFVNFTEIMSKNVN +RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQY+ATLELGLLGR+RGSSPERVPLPPPLY   P NGY RT  +NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGYPRTPRANSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           Q+RTP+H R N+GVGT  S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGE LE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRS IQTRI+LY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of MS015004 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 3.6e-164
Identity = 291/430 (67.67%), Postives = 346/430 (80.47%), Query Frame = 0

Query: 15  DSYGYDNSAAWDQ---FSYPPPSPYPQQ------YPYTPSSDYGSQTPQPQRRLDRKYSR 74
           +SY Y    ++ Q   ++ PPP  Y  Q      Y   PS  YGS     ++RL+RKYS+
Sbjct: 42  ESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPSYSAPPSQSYGSDN---KKRLERKYSK 101

Query: 75  IADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQ 134
           I+D+YSSL++VT ALA+AGLESSNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQ
Sbjct: 102 ISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQ 161

Query: 135 AISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVPNL 194
           AI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSF   DR CNGFEEVL RY+E+VP L
Sbjct: 162 AITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQL 221

Query: 195 RLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDAIV 254
           +LAGPTSFAPI++MA TIVE++GGQYHVL+IIADGQVTRSVDT +GQLSPQE+KT+DAIV
Sbjct: 222 KLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIV 281

Query: 255 KASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTEFA 314
           +AS+ PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQFVNFTEIM+KN   S ++TEFA
Sbjct: 282 QASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFA 341

Query: 315 LAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSRTP 374
           L+ALMEIP QYKAT+EL LLGR+ G  PER PLPPP+ G + S    + S   SF+   P
Sbjct: 342 LSALMEIPQQYKATIELNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVP 401

Query: 375 AHSRNNKGVGTA---PSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICR 433
            H      V ++   P +SS  DNQLCPICL+NPKDMAFGCGHQTCC+CG +L++CPICR
Sbjct: 402 PHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICR 461

BLAST of MS015004 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.2e-159
Identity = 289/442 (65.38%), Postives = 343/442 (77.60%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDN--------SAAWDQFSYPPPSPYPQQYPYTPSSDYGSQT 60
           MGG +SKE  R       Y+         S+AWDQ SY     +P   P + S + G QT
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQSSYYQTPNHPSASPVS-SYNSGRQT 60

Query: 61  PQPQRRLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRS 120
           P   + L+RKYSRIADNY S+DEVT+AL+ AGLESSNLIVGID TKSNEWTG+RSF R+S
Sbjct: 61  P---KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKS 120

Query: 121 LHHIGNGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFE 180
           LH IG   NPY+QAISIIG+TLSVFDEDNLIPC+GFGDA+THDQDVFSF  +D  CNGFE
Sbjct: 121 LHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFE 180

Query: 181 EVLHRYREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQ 240
           EVL  YRE+VP LRL+GPTSFAPI+E A TIVE++GGQYHVLLIIADGQVTRSVDT +G 
Sbjct: 181 EVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGG 240

Query: 241 LSPQERKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMS 300
            SPQE++TIDAIV+ASEYPLSI+LVGVGDGPWD M++FDDNIPARAFDNFQFVNFT+IMS
Sbjct: 241 FSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMS 300

Query: 301 KNVNPSRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYS 360
           KN++P+R++ EFAL+ALMEIPSQYKATLELGLLG++ G  P+R+ LPPP Y         
Sbjct: 301 KNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQSMRNSP 360

Query: 361 RTSHSNSFQSRTPAHSRNNKGVGTAPSSSSHYDN--QLCPICLTNPKDMAFGCGHQTCCD 420
           RTS S SFQ++       + GV + P S++H ++  Q CP+CL + K+MAF CGHQTC  
Sbjct: 361 RTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAG 420

Query: 421 CGENLELCPICRSFIQTRIKLY 433
           CGE+L +CPICRS I  RIKLY
Sbjct: 421 CGEDLHVCPICRSSISVRIKLY 433

BLAST of MS015004 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 5.1e-155
Identity = 282/448 (62.95%), Postives = 339/448 (75.67%), Query Frame = 0

Query: 9   PSRREFDSYG--YDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQ------------ 68
           P+     SYG  Y       Q + PPP  Y      +P+S YG Q  Q            
Sbjct: 54  PAPSPASSYGPQYSQEGYASQPNNPPPPTYAP--APSPASSYGHQYSQEGYASAASQPNY 113

Query: 69  ----------PQRRLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTG 128
                      +++ DR+YS+I+DNYSSL +V+ AL +AGLESSNLIVGIDFTKSNEWTG
Sbjct: 114 PPPPSQSQVADRKKFDRRYSKISDNYSSLLQVSEALGRAGLESSNLIVGIDFTKSNEWTG 173

Query: 129 SRSFNRRSLHHIGNGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEH 188
           ++SFNR+SLHH+ N  NPYEQAI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSFY  
Sbjct: 174 AKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPE 233

Query: 189 DRTCNGFEEVLHRYREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTR 248
            R CNGFEEVL RYRE+VP L+LAGPTSFAPI+EMA T+VE++ GQYHVL+IIADGQVTR
Sbjct: 234 GRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTR 293

Query: 249 SVDTHHGQLSPQERKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQF 308
           SVDT HG+LSPQE+KT+DAIVKAS  PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQF
Sbjct: 294 SVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQF 353

Query: 309 VNFTEIMSKNVNPSRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYG 368
           VNFTEIMSKN + SR++TEFAL+ALMEIP QYKAT+EL LLG + G+ P+R+PLPPP+  
Sbjct: 354 VNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATIELNLLGVRNGNIPQRIPLPPPV-- 413

Query: 369 AAPSNGYSRTSHSNSFQSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCG 428
              S     +S   +F+   P +   +K + +A       D QLCPICL+NPK+MAFGCG
Sbjct: 414 --QSGSSFSSSRIPNFEPSVPPYPFESKQMSSAD------DIQLCPICLSNPKNMAFGCG 473

Query: 429 HQTCCDCGENLELCPICRSFIQTRIKLY 433
           HQTCC+CG +L++CPICR+ IQTRIKLY
Sbjct: 474 HQTCCECGPDLKVCPICRAPIQTRIKLY 489

BLAST of MS015004 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-121
Identity = 212/372 (56.99%), Postives = 265/372 (71.24%), Query Frame = 0

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           +KY+ I D +SSLD+V+ AL +AGLESSNLI+G+DFTKSNEWTG  SF+ + LH +G   
Sbjct: 51  KKYALIPDRFSSLDQVSKALREAGLESSNLILGVDFTKSNEWTGKTSFDGKCLHALGETS 110

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYE+AI +IGQTL+ FDEDNLIPCFGFGD++THD++VF F+  +  C+GFEEVL  Y+ 
Sbjct: 111 NPYEKAIFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKR 170

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           + PNLRL+GPTS+ P+++ A  IVEK  GQ+HVL+I+ADGQVTR  D   G+LS QE+ T
Sbjct: 171 IAPNLRLSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTT 230

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           IDAIV AS Y LSI+LVGVGDGPW+ M++FDD IP R FDNFQFVNFTEIM++N   S +
Sbjct: 231 IDAIVNASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAK 290

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           +T FALAALMEIP QY+A +EL LLG+Q G +   VP PPP+    P+N           
Sbjct: 291 ETAFALAALMEIPFQYQAAIELRLLGKQTGLAKTIVPRPPPIPYTPPTNA---------- 350

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
                        + +  S +S    Q CPICLTN KD+AF CGH TC DCG  +  CPI
Sbjct: 351 ------------ELPSTASPASPEQTQSCPICLTNRKDVAFSCGHMTCGDCGSKISNCPI 400

Query: 421 CRSFIQTRIKLY 433
           CR  I  R+KLY
Sbjct: 411 CRVRITNRLKLY 400

BLAST of MS015004 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.3e-118
Identity = 218/369 (59.08%), Postives = 267/369 (72.36%), Query Frame = 0

Query: 64  SRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPY 123
           S IAD++SSLD+V ++L +AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG  QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 124 EQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVP 183
           E+AISIIG+TLS FDED+LIPCFGFGD +T DQ VFSFY  +++C+G E  + RYRE+VP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 184 NLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDA 243
           +L+L+GPTSFAP+++ A  IVE+   QYHVL+IIADGQVTR+ D   G+LSPQE  T+++
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNS 191

Query: 244 IVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTE 303
           I+ AS YPLSI+LVGVGDGPWD MK+FDDNIP R FDNFQFVNFT+IMS++ + ++++  
Sbjct: 192 IMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAA 251

Query: 304 FALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSR 363
           FALAALMEIP QYKATL L      R S     PLPPP       N      +    Q  
Sbjct: 252 FALAALMEIPFQYKATLSLN-RKPVRSSHQHHKPLPPPPEVIERDNAVRSVPN----QMT 311

Query: 364 TPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICRS 423
             A   +     T P         +CPICLTNPKDMAF CGH TC +CG  +  CP+CR 
Sbjct: 312 ETAEKSDRLAPSTVP---------VCPICLTNPKDMAFSCGHTTCKECGVVITTCPLCRQ 366

Query: 424 FIQTRIKLY 433
            I TRI+LY
Sbjct: 372 PITTRIRLY 366

BLAST of MS015004 vs. ExPASy TrEMBL
Match: A0A6J1DFL9 (E3 ubiquitin-protein ligase RGLG2-like OS=Momordica charantia OX=3673 GN=LOC111019656 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.5e-253
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD
Sbjct: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ
Sbjct: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE
Sbjct: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT
Sbjct: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR
Sbjct: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF
Sbjct: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI
Sbjct: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRSFIQTRIKLY
Sbjct: 421 CRSFIQTRIKLY 432

BLAST of MS015004 vs. ExPASy TrEMBL
Match: A0A1S3BDV1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103488946 PE=4 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.0e-215
Identity = 375/435 (86.21%), Postives = 396/435 (91.03%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPP--PSPYPQQYPYTPSSDYGSQTP-QPQR 60
           MGG+NS+E  +REF SYG  NS+ WDQ++YPP  PSPY QQYPYT  S YGSQTP QP+R
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGYGSQTPQQPRR 60

Query: 61  RLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDRKYSRIADNY SLDEVT+ALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHR 180
           N  NPYEQAISIIGQTLS FDEDNLIPCFGFGDASTHDQDVFSFY  DR CNGFEEVLHR
Sbjct: 121 NEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVLHR 180

Query: 181 YREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQE 240
           YRE+VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDTH+GQLS QE
Sbjct: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSLQE 240

Query: 241 RKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNP 300
           RKTIDAIV+ASEYPLSII VGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIM KNVN 
Sbjct: 241 RKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMLKNVNN 300

Query: 301 SRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHS 360
            RRQTEFALAALMEIPSQY+ATLELG+LGRQRGSSPERVPLPPPLY A  S+GY RT  S
Sbjct: 301 VRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSSGYPRTPCS 360

Query: 361 NSFQSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLEL 420
           NSFQ+RTP+  R N+GVGT PSSSS YDNQLCPIC+TNPKDMAFGCGH TCCDCGENLEL
Sbjct: 361 NSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGHMTCCDCGENLEL 420

Query: 421 CPICRSFIQTRIKLY 433
           CPICRSFIQTRI+LY
Sbjct: 421 CPICRSFIQTRIRLY 435

BLAST of MS015004 vs. ExPASy TrEMBL
Match: A0A5D3CUT6 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001680 PE=4 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.0e-215
Identity = 375/435 (86.21%), Postives = 396/435 (91.03%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPP--PSPYPQQYPYTPSSDYGSQTP-QPQR 60
           MGG+NS+E  +REF SYG  NS+ WDQ++YPP  PSPY QQYPYT  S YGSQTP QP+R
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGYGSQTPQQPRR 60

Query: 61  RLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDRKYSRIADNY SLDEVT+ALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHR 180
           N  NPYEQAISIIGQTLS FDEDNLIPCFGFGDASTHDQDVFSFY  DR CNGFEEVLHR
Sbjct: 121 NEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVLHR 180

Query: 181 YREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQE 240
           YRE+VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDTH+GQLS QE
Sbjct: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSLQE 240

Query: 241 RKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNP 300
           RKTIDAIV+ASEYPLSII VGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIM KNVN 
Sbjct: 241 RKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMLKNVNN 300

Query: 301 SRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHS 360
            RRQTEFALAALMEIPSQY+ATLELG+LGRQRGSSPERVPLPPPLY A  S+GY RT  S
Sbjct: 301 VRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSSGYPRTPCS 360

Query: 361 NSFQSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLEL 420
           NSFQ+RTP+  R N+GVGT PSSSS YDNQLCPIC+TNPKDMAFGCGH TCCDCGENLEL
Sbjct: 361 NSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGHMTCCDCGENLEL 420

Query: 421 CPICRSFIQTRIKLY 433
           CPICRSFIQTRI+LY
Sbjct: 421 CPICRSFIQTRIRLY 435

BLAST of MS015004 vs. ExPASy TrEMBL
Match: A0A6J1HP79 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita maxima OX=3661 GN=LOC111466460 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 364/432 (84.26%), Postives = 394/432 (91.20%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MG +NSKE  +REFDS G+ NS+ W   +YPPPSPY QQY Y   SDYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIAD+Y SLDEVT+ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDN+IPC+GFGDASTHDQ+VFSFY++DR CNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           +VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDT++GQLS QER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           +DAIV+ASEYPLSIILVGVGDGPWDMMK FDDNIPARAFDNFQFVNFTEIMSKNVN +RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQY+ATLELGLLGR+RGSSPERVPLPPPLY   P NGY RT  +NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGYPRTPRANSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           Q+RTP+H R N+GVGT  S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGE LE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRS IQTRI+LY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of MS015004 vs. ExPASy TrEMBL
Match: A0A6J1G0Z9 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita moschata OX=3662 GN=LOC111449704 PE=4 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 361/432 (83.56%), Postives = 391/432 (90.51%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60
           MG +NSKE  +REFDS G+ NS+ W   +YPPPSPY Q+Y Y    DYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRFDYGSQTPLPQRRLD 60

Query: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           RKYSRIAD+Y SLDEVT+ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDN+IPC+GFGDASTHDQ+VFSFY+ DR CNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDDDRICNGFEEVLHRYKE 180

Query: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240
           +VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDT++GQLS QER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300
           +DAIV+ASEYPLSIILVGVGDGPWDMMK FDDNIPARAFDNFQFVNFTEIMSKNVN +RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360
           QTEFALAALMEIPSQY+ATLELGLLGR+RGSSPERVPLPPPLY   P N Y RT  +NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNSYPRTPRANSF 360

Query: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420
           Q+RTP+H R N+GVGT  S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGE LE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIKLY 433
           CRS IQTRI+LY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of MS015004 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 579.3 bits (1492), Expect = 2.5e-165
Identity = 291/430 (67.67%), Postives = 346/430 (80.47%), Query Frame = 0

Query: 15  DSYGYDNSAAWDQ---FSYPPPSPYPQQ------YPYTPSSDYGSQTPQPQRRLDRKYSR 74
           +SY Y    ++ Q   ++ PPP  Y  Q      Y   PS  YGS     ++RL+RKYS+
Sbjct: 42  ESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPSYSAPPSQSYGSDN---KKRLERKYSK 101

Query: 75  IADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQ 134
           I+D+YSSL++VT ALA+AGLESSNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQ
Sbjct: 102 ISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQ 161

Query: 135 AISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVPNL 194
           AI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSF   DR CNGFEEVL RY+E+VP L
Sbjct: 162 AITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQL 221

Query: 195 RLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDAIV 254
           +LAGPTSFAPI++MA TIVE++GGQYHVL+IIADGQVTRSVDT +GQLSPQE+KT+DAIV
Sbjct: 222 KLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIV 281

Query: 255 KASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTEFA 314
           +AS+ PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQFVNFTEIM+KN   S ++TEFA
Sbjct: 282 QASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFA 341

Query: 315 LAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSRTP 374
           L+ALMEIP QYKAT+EL LLGR+ G  PER PLPPP+ G + S    + S   SF+   P
Sbjct: 342 LSALMEIPQQYKATIELNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVP 401

Query: 375 AHSRNNKGVGTA---PSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICR 433
            H      V ++   P +SS  DNQLCPICL+NPKDMAFGCGHQTCC+CG +L++CPICR
Sbjct: 402 PHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICR 461

BLAST of MS015004 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 579.3 bits (1492), Expect = 2.5e-165
Identity = 291/430 (67.67%), Postives = 346/430 (80.47%), Query Frame = 0

Query: 15  DSYGYDNSAAWDQ---FSYPPPSPYPQQ------YPYTPSSDYGSQTPQPQRRLDRKYSR 74
           +SY Y    ++ Q   ++ PPP  Y  Q      Y   PS  YGS     ++RL+RKYS+
Sbjct: 42  ESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPSYSAPPSQSYGSDN---KKRLERKYSK 101

Query: 75  IADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQ 134
           I+D+YSSL++VT ALA+AGLESSNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQ
Sbjct: 102 ISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQ 161

Query: 135 AISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVPNL 194
           AI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSF   DR CNGFEEVL RY+E+VP L
Sbjct: 162 AITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQL 221

Query: 195 RLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDAIV 254
           +LAGPTSFAPI++MA TIVE++GGQYHVL+IIADGQVTRSVDT +GQLSPQE+KT+DAIV
Sbjct: 222 KLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIV 281

Query: 255 KASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTEFA 314
           +AS+ PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQFVNFTEIM+KN   S ++TEFA
Sbjct: 282 QASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFA 341

Query: 315 LAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSRTP 374
           L+ALMEIP QYKAT+EL LLGR+ G  PER PLPPP+ G + S    + S   SF+   P
Sbjct: 342 LSALMEIPQQYKATIELNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVP 401

Query: 375 AHSRNNKGVGTA---PSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICR 433
            H      V ++   P +SS  DNQLCPICL+NPKDMAFGCGHQTCC+CG +L++CPICR
Sbjct: 402 PHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICR 461

BLAST of MS015004 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 579.3 bits (1492), Expect = 2.5e-165
Identity = 291/430 (67.67%), Postives = 346/430 (80.47%), Query Frame = 0

Query: 15  DSYGYDNSAAWDQ---FSYPPPSPYPQQ------YPYTPSSDYGSQTPQPQRRLDRKYSR 74
           +SY Y    ++ Q   ++ PPP  Y  Q      Y   PS  YGS     ++RL+RKYS+
Sbjct: 42  ESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPSYSAPPSQSYGSDN---KKRLERKYSK 101

Query: 75  IADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQ 134
           I+D+YSSL++VT ALA+AGLESSNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQ
Sbjct: 102 ISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQ 161

Query: 135 AISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVPNL 194
           AI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSF   DR CNGFEEVL RY+E+VP L
Sbjct: 162 AITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQL 221

Query: 195 RLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDAIV 254
           +LAGPTSFAPI++MA TIVE++GGQYHVL+IIADGQVTRSVDT +GQLSPQE+KT+DAIV
Sbjct: 222 KLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIV 281

Query: 255 KASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTEFA 314
           +AS+ PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQFVNFTEIM+KN   S ++TEFA
Sbjct: 282 QASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFA 341

Query: 315 LAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSRTP 374
           L+ALMEIP QYKAT+EL LLGR+ G  PER PLPPP+ G + S    + S   SF+   P
Sbjct: 342 LSALMEIPQQYKATIELNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVP 401

Query: 375 AHSRNNKGVGTA---PSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICR 433
            H      V ++   P +SS  DNQLCPICL+NPKDMAFGCGHQTCC+CG +L++CPICR
Sbjct: 402 PHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICR 461

BLAST of MS015004 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 579.3 bits (1492), Expect = 2.5e-165
Identity = 291/430 (67.67%), Postives = 346/430 (80.47%), Query Frame = 0

Query: 15  DSYGYDNSAAWDQ---FSYPPPSPYPQQ------YPYTPSSDYGSQTPQPQRRLDRKYSR 74
           +SY Y    ++ Q   ++ PPP  Y  Q      Y   PS  YGS     ++RL+RKYS+
Sbjct: 42  ESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPSYSAPPSQSYGSDN---KKRLERKYSK 101

Query: 75  IADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQ 134
           I+D+YSSL++VT ALA+AGLESSNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQ
Sbjct: 102 ISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQ 161

Query: 135 AISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYREVVPNL 194
           AI+IIG+TL+ FDEDNLIPC+GFGDASTHDQDVFSF   DR CNGFEEVL RY+E+VP L
Sbjct: 162 AITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQL 221

Query: 195 RLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKTIDAIV 254
           +LAGPTSFAPI++MA TIVE++GGQYHVL+IIADGQVTRSVDT +GQLSPQE+KT+DAIV
Sbjct: 222 KLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIV 281

Query: 255 KASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRRQTEFA 314
           +AS+ PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNFQFVNFTEIM+KN   S ++TEFA
Sbjct: 282 QASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFA 341

Query: 315 LAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSFQSRTP 374
           L+ALMEIP QYKAT+EL LLGR+ G  PER PLPPP+ G + S    + S   SF+   P
Sbjct: 342 LSALMEIPQQYKATIELNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVP 401

Query: 375 AHSRNNKGVGTA---PSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPICR 433
            H      V ++   P +SS  DNQLCPICL+NPKDMAFGCGHQTCC+CG +L++CPICR
Sbjct: 402 PHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICR 461

BLAST of MS015004 vs. TAIR 10
Match: AT1G67800.1 (Copine (Calcium-dependent phospholipid-binding protein) family )

HSP 1 Score: 564.3 bits (1453), Expect = 8.4e-161
Identity = 289/442 (65.38%), Postives = 343/442 (77.60%), Query Frame = 0

Query: 1   MGGRNSKEPSRREFDSYGYDN--------SAAWDQFSYPPPSPYPQQYPYTPSSDYGSQT 60
           MGG +SKE  R       Y+         S+AWDQ SY     +P   P + S + G QT
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQSSYYQTPNHPSASPVS-SYNSGRQT 60

Query: 61  PQPQRRLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRS 120
           P   + L+RKYSRIADNY S+DEVT+AL+ AGLESSNLIVGID TKSNEWTG+RSF R+S
Sbjct: 61  P---KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKS 120

Query: 121 LHHIGNGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFE 180
           LH IG   NPY+QAISIIG+TLSVFDEDNLIPC+GFGDA+THDQDVFSF  +D  CNGFE
Sbjct: 121 LHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFE 180

Query: 181 EVLHRYREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQ 240
           EVL  YRE+VP LRL+GPTSFAPI+E A TIVE++GGQYHVLLIIADGQVTRSVDT +G 
Sbjct: 181 EVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGG 240

Query: 241 LSPQERKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMS 300
            SPQE++TIDAIV+ASEYPLSI+LVGVGDGPWD M++FDDNIPARAFDNFQFVNFT+IMS
Sbjct: 241 FSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMS 300

Query: 301 KNVNPSRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYS 360
           KN++P+R++ EFAL+ALMEIPSQYKATLELGLLG++ G  P+R+ LPPP Y         
Sbjct: 301 KNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQSMRNSP 360

Query: 361 RTSHSNSFQSRTPAHSRNNKGVGTAPSSSSHYDN--QLCPICLTNPKDMAFGCGHQTCCD 420
           RTS S SFQ++       + GV + P S++H ++  Q CP+CL + K+MAF CGHQTC  
Sbjct: 361 RTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAG 420

Query: 421 CGENLELCPICRSFIQTRIKLY 433
           CGE+L +CPICRS I  RIKLY
Sbjct: 421 CGEDLHVCPICRSSISVRIKLY 433