MS014975 (gene) Bitter gourd (TR) v1

Overview
NameMS014975
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMitotic spindle checkpoint protein MAD2
Locationscaffold2: 359353 .. 361092 (+)
RNA-Seq ExpressionMS014975
SyntenyMS014975
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAAAAACAGAGACCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGGTATATGCTTCTGAAAACCCTTCTGATCTTAATTTTCTCTTTAATTCGGTTGCTAATCCAAGAATAACCTTGGCTGATTTCTTCTTTTTCCCTCTTTTCTTGAATTTTTTCTCATCCAGGATACGCTGCGAATAGGTATGAATATGGGATACGTTTCCTCTGTTCTTATTTTCATTTTCTGATCATGTAAATGTTCATTGATCGCAATTTTAACTGTGTAGCATCCTCTACAACCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTTAAGAAATATGGGCTTCCAATGTTGCTTACTCAAGACGAAGGTGTTAAATCCTTCATCACTAATCTAACTGCTCAGCTTTCTGGTAAATTCGCTTTCTCCAAATCAAAGATATGCATTGTGTAGTATGATTTATATTTATTGAGAAGTTTTTGAATCATGAAATTGGTTATAGAATGGCTTGAAGCTGGGAAGCTACAAAGGGTTGTGCTCGTCATATTGAGCAAAGCAAATAATGAGGTTCTTGAGAGGTGGAACTTCAGTATTGAAACTGATGGCGAAGTTGTCAAACAAGGGTATTAAATTCAACCAGAAACTTCTATTCCCTTTCATGTCTGAACTTCTTATCCTGATTACATGATCTGGCTGCAGCGTGTCGAGGGAAAAGAGTGATAAAGAAATTATGAGAGAGATTCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTATTTGCCCTGCCTTGATGAACCCTGCAAGTGTTTGCAACACTAACCATTATGAAATCGTTAGTTTATAATGATATTTAAGTCAATTTGAATGGTTTTGTCTGCTGTTTTTGTGGTTTTCAGGTGTATTTGATGTGTTAGCGTATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATTGAGAGTGACCCCAAACTGATTGCCAATCCACAAATGGTGAAGCTGCATTCTTTTGATACCAAGGTATAATCAAACTTATTTGTTCAGTTCATTTTTGGATCCACTGCTTTTGTTTATATATGGTGGTGTTGTGTTACTGCAGATTCACAGAGTGGACACACTTGTTTCTTACAAGAATGATGATGATGACTAAAAGCACTCCTATCGCTTTCGAGGACATTGTGAATCTTTTATTCACATTTTGTATTGCAATGTAATGATTCAATTAGAATTTCACCCGAATGGTTTGTTTGTTTGTTTGTTGTTCGGGTGGAACTTTGAGCAAAATGTGACTTTTTCTCTATAAAAAAACCTACTTTTTCGTTATAAGAACAAGGGTGACGACCGACCTGACCGAGTCTGCCAAGTAATTTCCTTGGCCAAAAATATACCCAAATTACTCGGTCAAAACAGAGAAAGAATCAATTCTAAATAACGAGCATCCATAATTAGAAACTTCCAAGTAAATTTGTCTTGAAAAATCCAAATTTTTATGATAGCTTGTTGAGAAAGAAGAAAAAGAAAGAAGAAAGAAATATGGGTTTAGATGATGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAATGACCCGCCACCATCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGCGGGTCCTCCGGCAAGAGCAAATTTGGGAAGACAATCTTGAGCTTGAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAA

mRNA sequence

ATGGCTTCAAAAACAGAGACCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGGATACGCTGCGAATAGCATCCTCTACAACCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTTAAGAAATATGGGCTTCCAATGTTGCTTACTCAAGACGAAGGTGTTAAATCCTTCATCACTAATCTAACTGCTCAGCTTTCTGAATGGCTTGAAGCTGGGAAGCTACAAAGGGTTGTGCTCGTCATATTGAGCAAAGCAAATAATGAGGTTCTTGAGAGGTGGAACTTCAGTATTGAAACTGATGGCGAAGTTGTCAAACAAGGCGTGTCGAGGGAAAAGAGTGATAAAGAAATTATGAGAGAGATTCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTATTTGCCCTGCCTTGATGAACCCTGCAAGTGTGTATTTGATGTGTTAGCGTATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATTGAGAGTGACCCCAAACTGATTGCCAATCCACAAATGGTGAAGCTGCATTCTTTTGATACCAAGAAGAAAAAGAAAGAAGAAAGAAATATGGGTTTAGATGATGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAATGACCCGCCACCATCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGCGGGTCCTCCGGCAAGAGCAAATTTGGGAAGACAATCTTGAGCTTGAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAA

Coding sequence (CDS)

ATGGCTTCAAAAACAGAGACCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGGATACGCTGCGAATAGCATCCTCTACAACCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTTAAGAAATATGGGCTTCCAATGTTGCTTACTCAAGACGAAGGTGTTAAATCCTTCATCACTAATCTAACTGCTCAGCTTTCTGAATGGCTTGAAGCTGGGAAGCTACAAAGGGTTGTGCTCGTCATATTGAGCAAAGCAAATAATGAGGTTCTTGAGAGGTGGAACTTCAGTATTGAAACTGATGGCGAAGTTGTCAAACAAGGCGTGTCGAGGGAAAAGAGTGATAAAGAAATTATGAGAGAGATTCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTATTTGCCCTGCCTTGATGAACCCTGCAAGTGTGTATTTGATGTGTTAGCGTATACTGATAAGGATCTTGCAGTTCCATTTACTTGGATTGAGAGTGACCCCAAACTGATTGCCAATCCACAAATGGTGAAGCTGCATTCTTTTGATACCAAGAAGAAAAAGAAAGAAGAAAGAAATATGGGTTTAGATGATGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAATGACCCGCCACCATCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGCGGGTCCTCCGGCAAGAGCAAATTTGGGAAGACAATCTTGAGCTTGAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAA

Protein sequence

MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKKKKKEERNMGLDDDGGLSWADQWDYNNDPPPSSSENDKKKNKSGGSSGKSKFGKTILSLKWMKELRKKSDNK
Homology
BLAST of MS014975 vs. NCBI nr
Match: KAA0034220.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 440.7 bits (1132), Expect = 9.2e-120
Identity = 233/263 (88.59%), Postives = 241/263 (91.63%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEE--RNMGLDDDGGLSWADQWDYNNDPPPSSSENDKKKNKSGGSS 240
           QMVKLHSFDTK  K +        DDDGGLSWADQWDYN DPPPSSS+NDKKKNK  GSS
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDDGGLSWADQWDYNPDPPPSSSDNDKKKNKD-GSS 240

Query: 241 GKSKFGKTILSLKWMKELRKKSD 262
            KSKF KTIL  KWMK+LRKKSD
Sbjct: 241 DKSKFRKTILGFKWMKDLRKKSD 260

BLAST of MS014975 vs. NCBI nr
Match: TYK15700.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 422.9 bits (1086), Expect = 2.0e-114
Identity = 233/304 (76.64%), Postives = 242/304 (79.61%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKE--------------------------------------ERNMGL 240
           QMVKLHSFDTK  K +                                       +N  L
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDGDDETRVDQVSESTQFIQILRSNQRILFSKNNNL 240

Query: 241 DD-----DGGLSWADQWDYNNDPPPSSSENDKKKNKSGGSSGKSKFGKTILSLKWMKELR 262
                  DGGLSWADQWDYN DPPPSSS+NDKKKNK  GSS KSKF KTIL  KWMK+LR
Sbjct: 241 SKIRNFADGGLSWADQWDYNPDPPPSSSDNDKKKNKD-GSSDKSKFRKTILGFKWMKDLR 300

BLAST of MS014975 vs. NCBI nr
Match: KAG7012753.1 (Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 402.5 bits (1033), Expect = 2.8e-108
Identity = 225/279 (80.65%), Postives = 238/279 (85.30%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT +KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVI+SKANNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTWIESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWIESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEE----RNMGLDDDGGLSWADQWDYNND--PPPSSSENDKKKN-- 240
           QMVKLHSFDTK  K +     +N   DDD GLSWADQWD N D  PPPSS+EN KKKN  
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDCDDD-GLSWADQWDNNPDPNPPPSSAENGKKKNIF 240

Query: 241 -------KSGGSSGKSK---FGKTILSLKWMKELRKKSD 262
                  + GGSS  S+   F K   +LKW+KELRKKS+
Sbjct: 241 KKKKKEKEEGGSSSSSEKNIFKKA--NLKWIKELRKKSE 274

BLAST of MS014975 vs. NCBI nr
Match: XP_022151752.1 (mitotic spindle checkpoint protein MAD2 [Momordica charantia])

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-97
Identity = 188/191 (98.43%), Postives = 189/191 (98.95%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVV+QGVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTWIESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWIESDPKLIANP 180

Query: 181 QMVKLHSFDTK 192
           QMVKLHSFDTK
Sbjct: 181 QMVKLHSFDTK 189

BLAST of MS014975 vs. NCBI nr
Match: XP_008446088.1 (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo])

HSP 1 Score: 357.8 bits (917), Expect = 7.8e-95
Identity = 186/207 (89.86%), Postives = 193/207 (93.24%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEERNMGLDDDGG 208
           QMVKLHSFDTK  K +      +DD G
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDG 205

BLAST of MS014975 vs. ExPASy Swiss-Prot
Match: Q9LU93 (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.4e-85
Identity = 163/195 (83.59%), Postives = 175/195 (89.74%), Query Frame = 0

Query: 1   MASKT-ETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT   KDIITL GSAAIVSEFF YAANSILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREK 120
            VKSF++NLT+Q+SEWLEAGKLQRVVLVI+SKA  EVLERWNF IETD EVV +GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIAN 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE   CVFDVLAYTD D+AVPFTWIESDPKLIAN
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDE--TCVFDVLAYTDTDVAVPFTWIESDPKLIAN 180

Query: 181 PQMVKLHSFDTKKKK 195
           PQMVKLH FDTK  K
Sbjct: 181 PQMVKLHGFDTKIHK 193

BLAST of MS014975 vs. ExPASy Swiss-Prot
Match: Q9XFH3 (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.7e-82
Identity = 156/194 (80.41%), Postives = 173/194 (89.18%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MAS++ +KDIITLRGSAAIVSEFFGYAANSILYNR +YPEESF +VKKYGL MLLTQDEG
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VK+FI +LT+QLSEWLEAGKLQR+VLVI+SKA +EVLERWNF+I TD EVV++G  +EKS
Sbjct: 61  VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIAS ITYLPCLDEP  CVFDVLAYTD D+  P TW+ESD KLI NP
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEP--CVFDVLAYTDTDVDAPGTWVESDAKLIDNP 180

Query: 181 QMVKLHSFDTKKKK 195
           QMVKLHSFDTK  K
Sbjct: 181 QMVKLHSFDTKIHK 192

BLAST of MS014975 vs. ExPASy Swiss-Prot
Match: Q556Y9 (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 7.2e-51
Identity = 107/197 (54.31%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           M +   +K  I+L+GS  IV+EFF Y+ N+IL+ RGLYP ESF RV KYGLP+L+T D+ 
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQG-VSREK 120
           +K ++ N+  QLSEWL +G +Q++VLVI      EVLERW F + TD  + K+G   R+K
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTD--IPKEGEAPRQK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIAN 180
            +KEIM EIQAI+RQI +S+T+LP L  P  C FD+L YT KDLAVP  W ESDPK I N
Sbjct: 121 PEKEIMNEIQAIIRQITASVTFLPLL--PNACTFDLLVYTSKDLAVPQKWEESDPKFITN 180

Query: 181 PQMVKLHSFDTKKKKKE 197
            Q VKL SF T   K E
Sbjct: 181 SQQVKLRSFTTTIHKVE 193

BLAST of MS014975 vs. ExPASy Swiss-Prot
Match: Q9Z1B5 (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 1.5e-45
Identity = 94/190 (49.47%), Postives = 127/190 (66.84%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MA +   +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L T D  
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPE 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           +  ++ N+  QL EWL    +Q++V+VI +  + EVLERW F IE D    ++GV REKS
Sbjct: 61  LIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
            K I  EI++++RQI +++T+LP L+    C FD+L YTDKDL VP  W ES P+ I N 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLE--VSCSFDLLIYTDKDLVVPEKWEESGPQFITNC 180

Query: 181 QMVKLHSFDT 191
           + V+L SF T
Sbjct: 181 EEVRLRSFTT 188

BLAST of MS014975 vs. ExPASy Swiss-Prot
Match: Q13257 (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.1e-43
Identity = 91/190 (47.89%), Postives = 124/190 (65.26%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MA +   +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L+T D  
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLE 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           +  ++ N+  QL +WL    +Q++V+VI +  + EVLERW F IE D         REKS
Sbjct: 61  LIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
            K I  EI++++RQI +++T+LP L+    C FD+L YTDKDL VP  W ES P+ I N 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLE--VSCSFDLLIYTDKDLVVPEKWEESGPQFITNS 180

Query: 181 QMVKLHSFDT 191
           + V+L SF T
Sbjct: 181 EEVRLRSFTT 188

BLAST of MS014975 vs. ExPASy TrEMBL
Match: A0A5A7STC2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002160 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.4e-120
Identity = 233/263 (88.59%), Postives = 241/263 (91.63%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEE--RNMGLDDDGGLSWADQWDYNNDPPPSSSENDKKKNKSGGSS 240
           QMVKLHSFDTK  K +        DDDGGLSWADQWDYN DPPPSSS+NDKKKNK  GSS
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDDGGLSWADQWDYNPDPPPSSSDNDKKKNKD-GSS 240

Query: 241 GKSKFGKTILSLKWMKELRKKSD 262
            KSKF KTIL  KWMK+LRKKSD
Sbjct: 241 DKSKFRKTILGFKWMKDLRKKSD 260

BLAST of MS014975 vs. ExPASy TrEMBL
Match: A0A5D3CWF2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001990 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 9.6e-115
Identity = 233/304 (76.64%), Postives = 242/304 (79.61%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKE--------------------------------------ERNMGL 240
           QMVKLHSFDTK  K +                                       +N  L
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDGDDETRVDQVSESTQFIQILRSNQRILFSKNNNL 240

Query: 241 DD-----DGGLSWADQWDYNNDPPPSSSENDKKKNKSGGSSGKSKFGKTILSLKWMKELR 262
                  DGGLSWADQWDYN DPPPSSS+NDKKKNK  GSS KSKF KTIL  KWMK+LR
Sbjct: 241 SKIRNFADGGLSWADQWDYNPDPPPSSSDNDKKKNKD-GSSDKSKFRKTILGFKWMKDLR 300

BLAST of MS014975 vs. ExPASy TrEMBL
Match: A0A6J1DEC0 (mitotic spindle checkpoint protein MAD2 OS=Momordica charantia OX=3673 GN=LOC111019657 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.1e-97
Identity = 188/191 (98.43%), Postives = 189/191 (98.95%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVV+QGVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTWIESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWIESDPKLIANP 180

Query: 181 QMVKLHSFDTK 192
           QMVKLHSFDTK
Sbjct: 181 QMVKLHSFDTK 189

BLAST of MS014975 vs. ExPASy TrEMBL
Match: A0A1S3BDR9 (mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.8e-95
Identity = 186/207 (89.86%), Postives = 193/207 (93.24%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VKSFI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEERNMGLDDDGG 208
           QMVKLHSFDTK  K +      +DD G
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDDDG 205

BLAST of MS014975 vs. ExPASy TrEMBL
Match: A0A0A0KPY5 (HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 3.2e-94
Identity = 184/205 (89.76%), Postives = 192/205 (93.66%), Query Frame = 0

Query: 1   MASKTETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKT TKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVI+SK+NNEVLERWNF IETDGEVV+QGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIANP 180
           DKEIMREIQAIMRQIASSITYLPCLDEP  CVFDVLAYTDKDLAVPFTW+ESDPKLIANP
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEP--CVFDVLAYTDKDLAVPFTWMESDPKLIANP 180

Query: 181 QMVKLHSFDTKKKKKEERNMGLDDD 206
           QMVKLHSFDTK  K +      +DD
Sbjct: 181 QMVKLHSFDTKIHKVDTLVSYKNDD 203

BLAST of MS014975 vs. TAIR 10
Match: AT3G25980.1 (DNA-binding HORMA family protein )

HSP 1 Score: 316.2 bits (809), Expect = 2.4e-86
Identity = 163/195 (83.59%), Postives = 175/195 (89.74%), Query Frame = 0

Query: 1   MASKT-ETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT   KDIITL GSAAIVSEFF YAANSILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREK 120
            VKSF++NLT+Q+SEWLEAGKLQRVVLVI+SKA  EVLERWNF IETD EVV +GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIAN 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE   CVFDVLAYTD D+AVPFTWIESDPKLIAN
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDE--TCVFDVLAYTDTDVAVPFTWIESDPKLIAN 180

Query: 181 PQMVKLHSFDTKKKK 195
           PQMVKLH FDTK  K
Sbjct: 181 PQMVKLHGFDTKIHK 193

BLAST of MS014975 vs. TAIR 10
Match: AT3G25980.2 (DNA-binding HORMA family protein )

HSP 1 Score: 301.2 bits (770), Expect = 8.1e-82
Identity = 158/195 (81.03%), Postives = 171/195 (87.69%), Query Frame = 0

Query: 1   MASKT-ETKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT   KDIITL GSAAIVSEFF     +ILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFF----CTILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKSFITNLTAQLSEWLEAGKLQRVVLVILSKANNEVLERWNFSIETDGEVVKQGVSREK 120
            VKSF++NLT+Q+SEWLEAGKLQRVVLVI+SKA  EVLERWNF IETD EVV +GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCKCVFDVLAYTDKDLAVPFTWIESDPKLIAN 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE   CVFDVLAYTD D+AVPFTWIESDPKLIAN
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDE--TCVFDVLAYTDTDVAVPFTWIESDPKLIAN 180

Query: 181 PQMVKLHSFDTKKKK 195
           PQMVKLH FDTK  K
Sbjct: 181 PQMVKLHGFDTKIHK 189

BLAST of MS014975 vs. TAIR 10
Match: AT1G24405.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G67670.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-11
Identity = 38/60 (63.33%), Postives = 40/60 (66.67%), Query Frame = 0

Query: 205 DGGLSWADQWDYNNDPPPSSSEND---KKKNKSGGSSGKSKFGKTILSLKWMKELRKKSD 262
           D GLSWADQWDYN+DPPP+SS  D   KKK K      KS  GK IL  KWMK L KKSD
Sbjct: 2   DSGLSWADQWDYNSDPPPNSSNEDDKKKKKKKKKEDGSKSGIGKAILGFKWMK-LGKKSD 60

BLAST of MS014975 vs. TAIR 10
Match: AT1G67670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G24405.1); Has 18 Blast hits to 18 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 18; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 37/62 (59.68%), Postives = 41/62 (66.13%), Query Frame = 0

Query: 205 DGGLSWADQWDYNN-DPPPSSSEND----KKKNKSGGSSGKSKFGKTILSLKWMKELRKK 262
           D G SWADQWDYNN DP PSS++ D    K K K      KS  GKT+L  KWMKELRKK
Sbjct: 2   DSGPSWADQWDYNNPDPLPSSTKEDESKKKNKKKKEEDGSKSSLGKTLLGFKWMKELRKK 61

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0034220.19.2e-12088.59mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa][more]
TYK15700.12.0e-11476.64mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa][more]
KAG7012753.12.8e-10880.65Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022151752.12.2e-9798.43mitotic spindle checkpoint protein MAD2 [Momordica charantia][more]
XP_008446088.17.8e-9589.86PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LU933.4e-8583.59Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ... [more]
Q9XFH32.7e-8280.41Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1[more]
Q556Y97.2e-5154.31Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX... [more]
Q9Z1B51.5e-4549.47Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma... [more]
Q132571.1e-4347.89Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD... [more]
Match NameE-valueIdentityDescription
A0A5A7STC24.4e-12088.59Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5D3CWF29.6e-11576.64Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1DEC01.1e-9798.43mitotic spindle checkpoint protein MAD2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3BDR93.8e-9589.86mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 ... [more]
A0A0A0KPY53.2e-9489.76HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G25980.12.4e-8683.59DNA-binding HORMA family protein [more]
AT3G25980.28.1e-8281.03DNA-binding HORMA family protein [more]
AT1G24405.14.4e-1163.33unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G67670.11.3e-1059.68unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036570HORMA domain superfamilyGENE3D3.30.900.10HORMA domaincoord: 1..197
e-value: 3.9E-70
score: 237.4
IPR036570HORMA domain superfamilySUPERFAMILY56019The spindle assembly checkpoint protein mad2coord: 10..194
IPR003511HORMA domainPFAMPF02301HORMAcoord: 16..190
e-value: 7.0E-21
score: 74.8
IPR003511HORMA domainPROSITEPS50815HORMAcoord: 14..200
score: 41.716042
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 194..246
IPR045091Mad2-likePANTHERPTHR11842MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2coord: 1..194
IPR027097Mitotic spindle checkpoint protein Mad2PANTHERPTHR11842:SF11MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2Acoord: 1..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014975.1MS014975.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling