MS014956 (gene) Bitter gourd (TR) v1

Overview
NameMS014956
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmannosyl-oligosaccharide glucosidase GCS1-like
Locationscaffold2: 210666 .. 221663 (-)
RNA-Seq ExpressionMS014956
SyntenyMS014956
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCGGACATAGTCGACGGAATCCTCGAAGCCGAATTAAATCATCCTCCGCCGTCGACAGCGGCGACGACGACGCCGTCCGTCAATCTCCGGATATCTCTACACGCCACAGAGATAGAACTAAATCGCGTCACTCAATTCGCATTTTCAATGTTAATCTCAAGGTCATGGTAGGTTTTAGCTTCATCGCGTTCTTCGTCATACTGTATTTGATCAGAAGCCTGATGGCAGTCGGAGATCATCAGACTCCTAGGGTTATTACGCCATTTCCTGCTCCAAGAGTTATCGACCTTCCTCAGGTGGACACACTTCTTCCATTATTTCTGTTTTGCTATTTATATTTTTAGTTCCAACTCTTATGCGGTTTTCGAGACAGTTTCAAGGGGAACATAAAGAGAGCTTGTACTGGGGAACTTATCGACCTCATCTTTATCTTGGAATACGAGCCAGGTACTTTGTTGAGTTGAAAATAAAGTCTCGTCGTGTTTTCAACAATTGAACTGAGTTATGTGTTCGTAATTCCAGGATGCTCAACTATTCAATTTGAGTTTGTAGGAAGCACTGTGTTGTACCACATAACCTTAAGCGTGTTTGGTGGTTGGAATGGCTCGATTCTATTTGTTGTGTTATGGAAATCAATTTATGCACTCCAAATCATTTTAAAAAGTCCGTACATATTGTTGGATTATTATAAACGACTTTATAACATGTCTAATAAATATAACAAAAAATGTCTAATATAAACGAATTTCTAAGATTAAAAGCTATTGAGGTAGTTGCATTTTTTCTAAGGCGAGTGGTCAACTGTAGCTGTGTTGTTAGATTTTAATTTTGAATTAACTATTTTAGAGTTTCTATCTCCATGATTCCGTTACATAATGATGGTTTACTGTAATGATAATCTAGACATCAGCCAATTCTTCTACATCTATTTTCAATTAAAGGTACAAAGGAACACATGCACTTGGCGTAAGTGCGATCACATATTTTACTCTTCTCCACTCTATTAAATGGTTGGACGAATACATAGTTGTTTAGCTAATAAATTGTTCTCTTATCTCTCAGGACTCCACGTTCATTGCTTGCTGGCTTGATGTGGTTAGGTGTTAAGAATGGAAGATATTTCATGAGGCATGTGTGCCAAGATTCCGACGAACTAAGCTCATATGGTTGGACTCATCATAATGCACGTGATTTTGGACATCAAGTTCTTGTTGACCAGGACATGACTTTGGCAACAAGTTTTCTGAAAACAAAGGAGAGTGGTAGTGGCTATGGTGGAGATTGGACTGTTCGCATTGAAGTTAATAGCAAGAAGTAAATGGCTATTAATAGAAGAGTATTTTAAGCATTGTTTGCATTGATGTGGCTTGAAATACTAACTTGTTAGTGAGTAGATGTATGATAACTGGATTGGAACTTATTTTGTGGACAGTTGTCGTTGGTAGTAGGTTAGTTCACTAAAGATCTAGCCTTGCATGTAAAGTCCTTGATGAACATTTGTCGACTTCTTGTTCCTTCCTTTGATTGGGGATTAATAATTCACTTTATTCATATGCTTCACATATTGGTAAATGATTGGACTACTAGGTGCACATATTCTCACACGCTAAAAATATCTTTCACCTGTAATGGGTAATGTCTAGCTTGGGATTTTTCTTGTGCATCCATCTTCAGAAGAAAATTAGGCATCGGAAGGTGTTAGAAGAAAAATGAAGTGTATTTTGCATTTGAAAAAAATTCTCGTAATATAAATATAAATGATCTGAGGTATTATTGAAAGTTAGTTTTTTCTTGCCCTGTAAGTAATGTACTCACGTTGAAGCATATATATCAATAGAATCTTGCATTGCTGACCATATGGTTATGTCATCTAACACATTTTCCACTTTTTGATGTACAAGTCGTCTTCTTATTGAGCCACTTGTACTGGAACCTGTCCTGCAGGATTCAGAATCAGACTATTAGTTTAATTGAGTCGTGGACTTTGTGAATATTTGTTAAATTTGTTTTAACTTGCAACTTACATGATGTGTAGGCTTGAGGCTTGGAATAGTTTTTTTCCCCTTTTGAGAATATTGTTGATTTTATCATGCTTCTTTAGTAAAGATGAACAAGTTCTTATTATTTATTGCTGAATTTGCTTTTTTTTCCCCTTTTGAGAATATGGTTGATTTTATCATGCTTCTTTAGTAAAGATGAACAAGTTCTTATTATTTATTGCTGAATTTGCAGATCTGAGGAGGCTGATGAAATGTTAAGAAGTGGACATCTTTTTTTCTATTTGGCAGATGAAGATGGAAATTCTTTAAGTTTGAGTAGTGATGCCATGGACATGCATGAGACTTCACTCCTAGCATCAGGTTCACGAAGCGATATTGGAAATTGGCAGTTACATTTAAAATCTAGGGTAAATTCCTGAAAGTAAAAGCATGAGGTCTCTCTAGATCTTTTTTTGAAAATTTTTCTTTTTAAAAATAATTTTCTTAGTTTCTTCTCTGGATTCTTTTCAAATGAGGAAGTATTATTTCTAGTGTGAGAAATTTCCTACGTTAGATTTATATGAATTCTTATGGACTAGTGTTGGCATTACTACTTCCAATCCACTTGCAATTCCTGAAGAAAGAAAATGTGCTTTTTTGCAATCCAAAAAAAAGCATTAAATTTGGTCATCTTCCAATCTATTGACTCTCTTGAGCTATATATTGTTTTGATCATCTTCCATTCTATTTTCTGCATTTTTTACTCTTTTAACGGGACCACATCAGTAAAGATCTCCATAATCATCTGTTCTGCTGTAGCTTTCAACCCTCAATCCCTCACATATCAGTCAAATAGCTTAATGAACATCTGATAAGATTTCTCTTTCTCATAATCGATTGTTGAAATTAAACTCTTTAATTTCCTAAGTAATTTTAGTCTGCCCCTTTAATGGGGTACCATTTGGATTCCTTTAACTGTCAAGTTAGAAAGCCTTCCATCCCATCCCCCAGTTGATTTCTCCCCCTCAAAATCTCAGAATTGGTGTGGTTATTGTAATTGGGATTGCTTGTGACATAATCTTCTTTGTTTTCTCATACTATTTGCATTTTTTTACTTGATCATTGTGGTGAATCTGGTGATATCATTTTTTTTTTCCTTTCATATATGGTTGCCCCTGCAGGAGGATGTGAAAATCCATTATAGTGGGTTTAAGACTGCACATTTTCACAATTTATCTGAACTTGTCCAGGAAAATCTTGGTACACAAGTAGGTTACCCTTTTTTCTCCTCCTTTTGTTTTAAATACATGTGGATGCCCCATGATGTCTGATTTTTTCCCCTGAATAATCTTATGGGGTTTACGTATCTGTGATGGACACTGCACTAATATTTCTTTGCATTATCTGTTTTACCAGGCAAGAAAATTTGGTCGACTGCAGCTATCTGATACATCTGATGATTCTTCAAATATTTTAGTTTTCCAGGTTGCTTTTGTCATATCATGGAGAAAGTGGAATATTTGAACCATATGAGTGACATCATTTTCTTGTTTTCTTATGTGCGATTCATTTATTTGTATTTTGCAGATCTCCGGAAGAATTCCATTTAAAATAGATATGGCTTTCATTTCGGGAACTGGGTTTCCAGGTTCTAGAGAAGAGGAACGTGTCAACCGTCTTACAGGTGTTTACTGTGAATCTTATTTTGGATCCACAAGATCCAGTTAGTAGTTGCCCATTGGCATGGCCCAAATTTAGAATTGAGAAAGGAATTAAATCTTTGTGTGCTTGTGTGTTGATAAGAAACAACTGAACTGTAAAAAGTTGTGAAATTAGAAACGAGGATAAGGAATCATTAGCCTCAACAGTGCATCAAAGAAGAACATAGAAGAACTATCTAATTGATTTGAATTTTTTTTGTGTGTATGCGCTCAGTCATTCACGTTATAGGGAAACCATGTAGAAGTCAAGGGCAAAAGAAAATCCTTACAAAAATTATTTCATTCATTTCCACATGTTCTGGAGAGTCTAATTGTTTTTATGGTTATGTATGTTTACACAGGTATGTCAATTATTCATGAAAAGGTTTCTTATTTTTTCGTGGCTTCCATTCTTCATGTATTGTTTTTGTTTCCCCACTTCAATTAGGTTCATCATTAACTGATCGATTAAAGGAGAAACAATTAGAGTTTGATACCAAATTTGAGAAGTGCTTTGGTTTGGCAAACAAGGTACTTATAGTTTGGAGATATTCTCGATATATTTGTCATCTTATATTTTCTATATTTTGAATTTTTGTTGCATCTTCAATCATTTATATGTAATATTTATCTCTTCACGATGGAGCTGGATAAGGATAAACCAGTGCATGATTTATTTCAATTAGAAATTTAGTAGTATGTTGATATAATACTTCATTGAAATCTACAATGGCCAGCCATAAAGTTATTAAAAATAAGATTTAATTGGGTTTAATTGAGTAACTACTGGTGGATATAGCGCATAGCTGGATGTTATTTGGATAGCTAGAGCACCGACTCAAGATACCATTTCAAAACATCCAAAAAAGAAAGAAAAGGAAGATTATGGTGACCAGGAATGCTTTGAATAGGAACTGAAAAATATTACTATACCTCCAAACATTTCAATGCTTTTGATTTTCCTTTCAAGCAAGACACGGTATGAATGCTTTTAAAGATTATTTGAGATATCAGCTTTTAAAGATTATTTGAGATATGTGATATGATGGTTGTACACTCGCGATCACATTCAGCTGTAAATAAATTTTTATATCATGAAGCAAATTCTATTTTCAAAATGCAACATTTCCCAATAACAAATTAACGTTAAGTAACCAACAAAATATATAACAAGTTCATCAAATAATAATCTCTCATCTTAAACATAAGTTCTCATGTCACCCGTTTTTTAGCATTTAGTTTTTGTAGCGATCACTTGTGGGTTTTTCTGTAAAAAGGAAAATTAATTTCCTATCTTAGTTCTTTATTTTAAATACTTCTTGGTGGTAAAAATGTATGATCCATCCTTCAAGAAAAATGTGTCCCTTTGAAAAAGGTAGTAAGAATATCTGACACTTTAAACATGTATCTGAATTGTGTGTCAGAGATGTCTGACACTGATACACACGTACAGCAAAGCATCAAAAGTTGGATGTGTTATCCTATTAGGCAAAATGCATGTTTGGCAATCTTTTCCATAGTTTATGTTCAATACAATATATACGAAGTTCATCTAAAAAGTGGCAGAAGAAAAGCACGGGTTTCCAACTTCTAATTGATATTTGCTTCTTTGATGCTAGATTATATTCTGTTCAAGTTATTAATCTTGTAATTTTTCATTGATGGATCTAATCTTGCTATTATGCTCAGTTTGATTTAGAATCCACAACTGTTGGTAAGGCTGCTGTTGCAAACTTATTAGGTGGCATTGGCTATTTCTATGGCCAGTCCAAAATTGCACTCCCAGGTGCCTCTCATGTAAGTGATTGGAGCTTTTCCTCATTCATTGTCGGCATGTCTGGAAGATTTTGTAAACATCATGTCATTGTTACAAAAGCCTCCCCATTATGGATGTTTTTTCTCTCTTTTCCAGCTCACAAATCCTTCATTTATTTTATCGTTAAATAATCTCTATTTAATGACATCCCTCTCATAGTATGAGCAAGTATACCTGTTTTAAATGCTCCATCAGCCTCGATATTTATTCGAGCATTTTTATGAAATCTCCTTCTAAAATAATGAATCTTAAGGACTGGATTTTAGTGCTCTGGAAGTGAGTAGACATAGATACTGGATGATACAAATAAACTGATGATGTTTGTTTGGATGTTTGATAATTTTACAAGATGTGAGTGCCCATATTAGGCAAAGATAAAGAAGCAAAAGAATAGGGGAATGAGATTTCCTCAGCAAAGAAAAACAATCACAAATACGAACTCCATATACTCTGCAAACCTGGGGAACTGGGTAAAATTTTACAAATATTGCATTAAATATTAATAATTATTGAATCATATTCAATAGATCATTGAATAATGTTCAAATAATTATAGAATCCAATTCAATAATTATTGAAAATATGAGATTGAGTAGAATTAAGTAATTATTGAAGACTATTAAGTGATTATTACTACTTTTGTAATTACGTAAAATTGTTCGTAAAATTGTTGCTACATATGAAAACATCTATCTAAAATTTATCATTCTTGTGATTGGCCTGAGAAATAAACATAATCTACAGATTTAACATTCTCGCATCAATATTATTGAACTTGTATTTTGTAATTTTCCATAACATTGTTCATTTTTATTTCTATTTTTCAGTCTCCATGTTTCTATTTGTATTCTATTCCTTTTGCATTGAAACCTGTGAACATTTGTATCTTATTAATTTGACCTCTGAGGAAATTCTGCGCTAAATTTATTGCAGCCCAGAAGTCGTGATAGTTTCCTTTTTTATTGGCCTGCTGAGCTTTACACTGCTGTTCCATGCCGTCCTGTCTTCCCTAGGGGATTTTTGTGGGATGAAGGCTTTCATCAGCTGCTGATCTGGCAAGTGCTTATTGTTTCTAGATGTGCAGTTCTTTTTTATATCATCAACTGCTGATAGATTATTTGTTTCTTGGATATGAAAAATTATTGACACCTTTACCTACATTTTTTCCAATGCTGAACAGGCGATGGGATATTTATATTTGCTTAGATATTCTTGGTCACTGGTTGGATCTAATGAATATCGATGGATGGATTCCACGCGAACAAATATTAGGGGCTGAAGCTCTCAGGTGATAGCATGTTCAAATTTGTGATGTTAAATTATGCTCAACCTGTCCTGAAGTATCTTCCACTTTGTTTTTATAATTTTATTGCAGTAAGGTTCCGGAGGAATTTATTCCTCAGCATCCAAGCAATGCGAACCCACCAGCATTATTTCTAGTCATGCGTGGTAATTTTTTTATAATTTTATTGCAGTAAGGTCCTGGAGGAATTTATTCCGCAGCGACCAAGCAATGTTAACCCACCAGCATTATTTCTAGTCCTGTGTGACAATATATGTCTATTCCTGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAAGATGTTAAAGATATTGAGAAAAGAGAGTTTGGGCGAGACCACCCCTGGATTAGTAGGGTGGACCATGATCATAATGCTTCGAGTGGAGTCCCAATACCCTAGTTATTTAAAAAAATGAAAAAGAAAGGAAAGAGAGCATAATTTTAGGGAAATGAGAACAAAGAAAACAGAAATCACGATGGCATGCCAGTGGTGTTGGGAGACATTCATAGTAAGATGTAATTAGAGTTTCACCCCTGTAATTTTCACTTGTATTTCTGATGGAGGAAAAGAAAGTTGTTTACAATGCAGAATGCTCCTTATATATCTGCTACATGAAAGATATATTGTATTGTAGATTATGCACGAAACTCTGAATTACTTCTTTCTTCTTTGTTTATATAAATAAGAAACAACAAATGTTGATGAACATTAATCGAAGGTCCAATATGAGGAACTTTTAAGAATCCTTAGAGACGATGAGTGCCTTGCTACCAATCCTTGCCACTACATTTGATGAGAGTTTATTTCTTGAAATCATATATCTTCTGCATTTCGCCAAACACTCCCATATACACTTGATTGTATACAGAAATCTGAGCATGCGTAAGTGATGTGGTGGTTTTAAACTTTTACAAGCTTTACATTTTCTGCTTTTGTATATAATCTTGTTCACCAGTAGAGATGAACTTACTTTATTATGGTAGTAGCATATAATTGTGATCTGTAACATAAGTTATAAAAAACTTTGGTTAAATCTTAGTCGCTATGTGGTGATTTGATTTGTTTCTTCTTGTAGAGTTAGTCCATGGCCTAAAGAGAAATGCATTTACGGCCAAAGAGAGCTCGGAGATATCTTCATTCTTAGAAAGAGCTTTTGTCCGCTTGGAAGCTTGGTTCCAGTGGTTCAATACTACACAGCCAGGTGTCTGCATACATGTGCCTTTTGTTTTTGCATGTTCTATTTATTTCTAGGAAGGTAGTCGGCCACTCAATGGTTCTTTCACTATGCATGAGAACATATCTTTTTTTAATCAAGATATATGTATGCGCGTGTGTGAGGAACACAGGAAGAGAGGAAAGCAGCTATTACTGGCATGGAAGAGACAGCTCAACAATTCGTGAACTTAACCCTCAGGTTTCATTCTATACTCTTCTCTCTTTTTCTGTCGTACACTATTGGTGAAGTAAACAGAGCCCTCCATCACTTGTACATCTTTACCTTATCAATACGACTATCCAATTATTAGTTTTTCCATCGTCAATTATTGAAAAAAAAAATATATTGATCTTTATAAATTACTTGGGGTAATTTAGATTCTCATGCTGGTATGTGGCGTGGTGATTGTGAAAGGAAATCCTTGTTTGAAAACAACTCTTATTTGTACATGGTGCTGGGAAGGCCAATAATCCATTTTCTTTTTTTTTTTTTTTTTTGAAAGAAACAAAGAACCAAGAAACCCAAAAAGGGGGAGACAACCTAAACGCGGAGAGTGGAGAGAACGCTCCCCTAAAGACTATACAATCACAACTTTCCAACCTTGCATAATAAAAAACAAACAAACTGTAATTACAAAGTAGATTAGTGCTTTGTAATTAAAAAGTGGATTGAAGCCAATCATGAAGCCATATGCTTGATATTCACCATTTTCAGTTTAATTATACAATAGTAAAGAGAATAGAGAGAACTAAAGTAGATTAGTTATGCGCCTAAGTTAATTTTGTTGGACATCTGTATATGTTCAAAACTATTAACAAAATTGGAGTTCATCACGGGGCAAATCATCAAATATTTTAAAAGAATAAGGGAATTTCTGTGAACTTCTTTGTCTGTAGGACGTTACAGAAACATATTTCTCTTAGACTTCAAAATAGTGTCTTTGACATTTCTCTTATCTATGCATTATCAATATGCTAGACTTACCAACAAAGTATTACATGATATCAAATTGTTCGCACTTATAGACACTGATGTCTGGATTGGACGATTATCCTCGTGCTTCACACCCAAGTGAGGATGAGCGCCATGTGGACCTCCGATGCTGGATGCTTCTTGCTGCGGATTGCATGCATTCAATTTCTGAGCTATCTATGAAGGGAAAGGAAGTTGGAATGGTAATTCGTCAAGAGAGATTAACTTAGTATCTCATAGGTTTTCTTTTCTTCAACTTATCATGAACTTATGTGATGCAGGTATATAGTTTAGCCGCAAACACACTGTCGAATTTTGAGCTTCTGAATCAGGTTGGTTGTCACTTTCATCTCTTCTGGAAATGTATTTTATAACGTCTGTATGGATTCCTCAGGTTTCCTTCAACTCAAAATGTATTTATGTAGGTGATCTTTTTTTTTTTCAAATTTCATTTTAATATAGGAATACATATTAAGCTCTAGACCTCTAATATGACTATAATTGATGCCTTATCAGATGCACTTTGATGATGCTTATGGAACTTATTTAGATTTCGGAAATCACACCGAAAAGGTATGATCTCTGTATTTTCATTGTCTCTGCAATATGACTGTAATTTAGTAGTATCCTAGAAATCAAATCTACATATGTGGGTGGGTGGGGCATGAATTTTATTCTTTGAATTGCTGTACTTTTTTCTTCTAATCTATGTAGGCCAATTGGAAACACTCTTGTAATTCCCGGTTGTTTTGATGATTTCTCATTTGGTCTTTGGTACTTTTTTTTAATAAGAAACTTGTATGTATTAATCACAAAAGGCAAAGACCATCTAGGGGCAGGGGATGAGGGAGCCCCCTCCCAAGAGCCTAAAATAAAACCCCATATGCATATGAGGGATAAAATTCTTAGTTTCCACATATGAATTTGGCTTGTGAGGAGTTTATGAGGCTGTTCCTTTATTATGGATTAGGTATTGTTTGAGAAATTACAGAATGAACATTATCACAAAGGCTTCAAATTTCCATCTCCATTAAGGATATAGCTACTGAGTGTTAAGAGTCTATTCTTGTGTTTTGTAATGCAATGATTGATGTCATGTAGGTTCGTTTAAGTTGGAAAGAGGTGATGGTAGAGCAGGGATATACAACTCGACAACTTATCCGGGAAGTGTTAGAAACACCAACACTGAGAATGGTTCCTCATATCGGTTATGTCAGTCTTTTTCCATTGATGGGGAGGATCATTCCACCAGTAAGTTTCAGTAACATGAAATTATTCACATGTTTATTTTCCGGTGCTGAAGAGGCTTGAATTTTTCTCTTCCTCTCTCCAACAGGAATCTTGGATTCTTGGGAAACAGCTTGATCTTATTTCAAATCGAAGTGTATTCTGGACAAATTACGGGCTCCGTTCCCTGTCTAAAACAAGGTAACTATATCAGAACTTGTAATTCCTAATGAATTCTGCTGTGTATTATATGGACTAATTTGGCTCCCAAAACTCCATGCTGTAGCTCGTTATACATGAAATACAATACAGAGCATGATGCGCCTTATTGGAGAGGTACCATTTGGATGAACATGAATTACTTGATTCTTTCAGCACTCAACCATTATGCCACAGGTATCCGTAATGCCATCATTTCCTATCTAGACTCTAATGGGAACCTCGTTTTCTTTGCTTTAATTACCTTACCGTGACCCGAATTTCAGAGGATGGACCCTATAGAGAAAGAGCCCTAACTATTCGGGATGAGTTGAGAAGCAATTTGATTAGGTATTTTCCTCCTACAAACCACTTCTTTCTAATTGTTTCATTGCGTACAGGAAGCTTAGATTGTTTGGTTATTGGTGCAGAAATGTGGTTCGCAATTATCACCAGAGTGGATTCATATGGGAACAGTACAATCAAAAGACGGGGAAGGGAAAAGGTGCGCATCCATTTACTGGTTGGACGTCTCTGGTGCTGTTGATCATGGCAGAAGCTTATACAGAAAGT

mRNA sequence

ATGCCCGGACATAGTCGACGGAATCCTCGAAGCCGAATTAAATCATCCTCCGCCGTCGACAGCGGCGACGACGACGCCGTCCGTCAATCTCCGGATATCTCTACACGCCACAGAGATAGAACTAAATCGCGTCACTCAATTCGCATTTTCAATGTTAATCTCAAGGTCATGGTAGGTTTTAGCTTCATCGCGTTCTTCGTCATACTGTATTTGATCAGAAGCCTGATGGCAGTCGGAGATCATCAGACTCCTAGGGTTATTACGCCATTTCCTGCTCCAAGAGTTATCGACCTTCCTCAGTTTCAAGGGGAACATAAAGAGAGCTTGTACTGGGGAACTTATCGACCTCATCTTTATCTTGGAATACGAGCCAGGACTCCACGTTCATTGCTTGCTGGCTTGATGTGGTTAGGTGTTAAGAATGGAAGATATTTCATGAGGCATGTGTGCCAAGATTCCGACGAACTAAGCTCATATGGTTGGACTCATCATAATGCACGTGATTTTGGACATCAAGTTCTTGTTGACCAGGACATGACTTTGGCAACAAGTTTTCTGAAAACAAAGGAGAGTGGTAGTGGCTATGGTGGAGATTGGACTGTTCGCATTGAAGTTAATAGCAAGAAATCTGAGGAGGCTGATGAAATGTTAAGAAGTGGACATCTTTTTTTCTATTTGGCAGATGAAGATGGAAATTCTTTAAGTTTGAGTAGTGATGCCATGGACATGCATGAGACTTCACTCCTAGCATCAGGTTCACGAAGCGATATTGGAAATTGGCAGTTACATTTAAAATCTAGGGAGGATGTGAAAATCCATTATAGTGGGTTTAAGACTGCACATTTTCACAATTTATCTGAACTTGTCCAGGAAAATCTTGGTACACAAGCAAGAAAATTTGGTCGACTGCAGCTATCTGATACATCTGATGATTCTTCAAATATTTTAGTTTTCCAGATCTCCGGAAGAATTCCATTTAAAATAGATATGGCTTTCATTTCGGGAACTGGGTTTCCAGGTTCTAGAGAAGAGGAACGTGTCAACCGTCTTACAGGTTCATCATTAACTGATCGATTAAAGGAGAAACAATTAGAGTTTGATACCAAATTTGAGAAGTGCTTTGGTTTGGCAAACAAGTTTGATTTAGAATCCACAACTGTTGGTAAGGCTGCTGTTGCAAACTTATTAGGTGGCATTGGCTATTTCTATGGCCAGTCCAAAATTGCACTCCCAGGTGCCTCTCATCCCAGAAGTCGTGATAGTTTCCTTTTTTATTGGCCTGCTGAGCTTTACACTGCTGTTCCATGCCGTCCTGTCTTCCCTAGGGGATTTTTGTGGGATGAAGGCTTTCATCAGCTGCTGATCTGGCAAGCGTGGGATATTTATATTTGCTTAGATATTCTTGGTCACTGGTTGGATCTAATGAATATCGATGGATGGATTCCACGCGAACAAATATTAGGGGCTGAAGCTCTCAGTAAGGTTCCGGAGGAATTTATTCCTCAGCATCCAAGCAATGCGAACCCACCAGCATTATTTCTAGTCATGCGTGAGTTAGTCCATGGCCTAAAGAGAAATGCATTTACGGCCAAAGAGAGCTCGGAGATATCTTCATTCTTAGAAAGAGCTTTTGTCCGCTTGGAAGCTTGGTTCCAGTGGTTCAATACTACACAGCCAGGAAGAGAGGAAAGCAGCTATTACTGGCATGGAAGAGACAGCTCAACAATTCGTGAACTTAACCCTCAGACACTGATGTCTGGATTGGACGATTATCCTCGTGCTTCACACCCAAGTGAGGATGAGCGCCATGTGGACCTCCGATGCTGGATGCTTCTTGCTGCGGATTGCATGCATTCAATTTCTGAGCTATCTATGAAGGGAAAGGAAGTTGGAATGGTATATAGTTTAGCCGCAAACACACTGTCGAATTTTGAGCTTCTGAATCAGATGCACTTTGATGATGCTTATGGAACTTATTTAGATTTCGGAAATCACACCGAAAAGGTTCGTTTAAGTTGGAAAGAGGTGATGGTAGAGCAGGGATATACAACTCGACAACTTATCCGGGAAGTGTTAGAAACACCAACACTGAGAATGGTTCCTCATATCGGTTATGTCAGTCTTTTTCCATTGATGGGGAGGATCATTCCACCAGAATCTTGGATTCTTGGGAAACAGCTTGATCTTATTTCAAATCGAAGTGTATTCTGGACAAATTACGGGCTCCGTTCCCTGTCTAAAACAAGCTCGTTATACATGAAATACAATACAGAGCATGATGCGCCTTATTGGAGAGGTACCATTTGGATGAACATGAATTACTTGATTCTTTCAGCACTCAACCATTATGCCACAGAGGATGGACCCTATAGAGAAAGAGCCCTAACTATTCGGGATGAGTTGAGAAGCAATTTGATTAGAAATGTGGTTCGCAATTATCACCAGAGTGGATTCATATGGGAACAGTACAATCAAAAGACGGGGAAGGGAAAAGGTGCGCATCCATTTACTGGTTGGACGTCTCTGGTGCTGTTGATCATGGCAGAAGCTTATACAGAAAGT

Coding sequence (CDS)

ATGCCCGGACATAGTCGACGGAATCCTCGAAGCCGAATTAAATCATCCTCCGCCGTCGACAGCGGCGACGACGACGCCGTCCGTCAATCTCCGGATATCTCTACACGCCACAGAGATAGAACTAAATCGCGTCACTCAATTCGCATTTTCAATGTTAATCTCAAGGTCATGGTAGGTTTTAGCTTCATCGCGTTCTTCGTCATACTGTATTTGATCAGAAGCCTGATGGCAGTCGGAGATCATCAGACTCCTAGGGTTATTACGCCATTTCCTGCTCCAAGAGTTATCGACCTTCCTCAGTTTCAAGGGGAACATAAAGAGAGCTTGTACTGGGGAACTTATCGACCTCATCTTTATCTTGGAATACGAGCCAGGACTCCACGTTCATTGCTTGCTGGCTTGATGTGGTTAGGTGTTAAGAATGGAAGATATTTCATGAGGCATGTGTGCCAAGATTCCGACGAACTAAGCTCATATGGTTGGACTCATCATAATGCACGTGATTTTGGACATCAAGTTCTTGTTGACCAGGACATGACTTTGGCAACAAGTTTTCTGAAAACAAAGGAGAGTGGTAGTGGCTATGGTGGAGATTGGACTGTTCGCATTGAAGTTAATAGCAAGAAATCTGAGGAGGCTGATGAAATGTTAAGAAGTGGACATCTTTTTTTCTATTTGGCAGATGAAGATGGAAATTCTTTAAGTTTGAGTAGTGATGCCATGGACATGCATGAGACTTCACTCCTAGCATCAGGTTCACGAAGCGATATTGGAAATTGGCAGTTACATTTAAAATCTAGGGAGGATGTGAAAATCCATTATAGTGGGTTTAAGACTGCACATTTTCACAATTTATCTGAACTTGTCCAGGAAAATCTTGGTACACAAGCAAGAAAATTTGGTCGACTGCAGCTATCTGATACATCTGATGATTCTTCAAATATTTTAGTTTTCCAGATCTCCGGAAGAATTCCATTTAAAATAGATATGGCTTTCATTTCGGGAACTGGGTTTCCAGGTTCTAGAGAAGAGGAACGTGTCAACCGTCTTACAGGTTCATCATTAACTGATCGATTAAAGGAGAAACAATTAGAGTTTGATACCAAATTTGAGAAGTGCTTTGGTTTGGCAAACAAGTTTGATTTAGAATCCACAACTGTTGGTAAGGCTGCTGTTGCAAACTTATTAGGTGGCATTGGCTATTTCTATGGCCAGTCCAAAATTGCACTCCCAGGTGCCTCTCATCCCAGAAGTCGTGATAGTTTCCTTTTTTATTGGCCTGCTGAGCTTTACACTGCTGTTCCATGCCGTCCTGTCTTCCCTAGGGGATTTTTGTGGGATGAAGGCTTTCATCAGCTGCTGATCTGGCAAGCGTGGGATATTTATATTTGCTTAGATATTCTTGGTCACTGGTTGGATCTAATGAATATCGATGGATGGATTCCACGCGAACAAATATTAGGGGCTGAAGCTCTCAGTAAGGTTCCGGAGGAATTTATTCCTCAGCATCCAAGCAATGCGAACCCACCAGCATTATTTCTAGTCATGCGTGAGTTAGTCCATGGCCTAAAGAGAAATGCATTTACGGCCAAAGAGAGCTCGGAGATATCTTCATTCTTAGAAAGAGCTTTTGTCCGCTTGGAAGCTTGGTTCCAGTGGTTCAATACTACACAGCCAGGAAGAGAGGAAAGCAGCTATTACTGGCATGGAAGAGACAGCTCAACAATTCGTGAACTTAACCCTCAGACACTGATGTCTGGATTGGACGATTATCCTCGTGCTTCACACCCAAGTGAGGATGAGCGCCATGTGGACCTCCGATGCTGGATGCTTCTTGCTGCGGATTGCATGCATTCAATTTCTGAGCTATCTATGAAGGGAAAGGAAGTTGGAATGGTATATAGTTTAGCCGCAAACACACTGTCGAATTTTGAGCTTCTGAATCAGATGCACTTTGATGATGCTTATGGAACTTATTTAGATTTCGGAAATCACACCGAAAAGGTTCGTTTAAGTTGGAAAGAGGTGATGGTAGAGCAGGGATATACAACTCGACAACTTATCCGGGAAGTGTTAGAAACACCAACACTGAGAATGGTTCCTCATATCGGTTATGTCAGTCTTTTTCCATTGATGGGGAGGATCATTCCACCAGAATCTTGGATTCTTGGGAAACAGCTTGATCTTATTTCAAATCGAAGTGTATTCTGGACAAATTACGGGCTCCGTTCCCTGTCTAAAACAAGCTCGTTATACATGAAATACAATACAGAGCATGATGCGCCTTATTGGAGAGGTACCATTTGGATGAACATGAATTACTTGATTCTTTCAGCACTCAACCATTATGCCACAGAGGATGGACCCTATAGAGAAAGAGCCCTAACTATTCGGGATGAGTTGAGAAGCAATTTGATTAGAAATGTGGTTCGCAATTATCACCAGAGTGGATTCATATGGGAACAGTACAATCAAAAGACGGGGAAGGGAAAAGGTGCGCATCCATTTACTGGTTGGACGTCTCTGGTGCTGTTGATCATGGCAGAAGCTTATACAGAAAGT

Protein sequence

MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGFSFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAYTES
Homology
BLAST of MS014956 vs. NCBI nr
Match: XP_022151803.1 (mannosyl-oligosaccharide glucosidase GCS1-like [Momordica charantia])

HSP 1 Score: 1735.7 bits (4494), Expect = 0.0e+00
Identity = 844/854 (98.83%), Postives = 850/854 (99.53%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF
Sbjct: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL
Sbjct: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           G+RARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT
Sbjct: 121 GMRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLR+GHLFFYLADEDGNSLSLSSDA
Sbjct: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRTGHLFFYLADEDGNSLSLSSDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF
Sbjct: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFKIDM FISGTGFPGSREEERVNRLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMTFISGTGFPGSREEERVNRLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD
Sbjct: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDIYICLDILGHWLDLMNIDGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIYICLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESS+ISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSKISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+EESSYYWHGRDSS IRELNPQTLMSGLDDYPRASHPSED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEESSYYWHGRDSSPIRELNPQTLMSGLDDYPRASHPSED 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRL WKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. NCBI nr
Match: XP_022957119.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita moschata])

HSP 1 Score: 1584.7 bits (4102), Expect = 0.0e+00
Identity = 762/854 (89.23%), Postives = 807/854 (94.50%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSRIKSSSAVD   D AVRQ+PDIS RHRDR   R  IRIF+VNLKV+VGF
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPLIRIFDVNLKVLVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFGAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP  SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQHPSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQHPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL MKGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIMKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPQLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. NCBI nr
Match: XP_022997334.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita maxima])

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 764/854 (89.46%), Postives = 808/854 (94.61%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSRIKSSSAVD G D AVRQ+PDIS RHRDR   R  IRIF+VNLKV+VGF
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGGGDGAVRQAPDISRRHRDRNTLRPWIRIFDVNLKVLVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELSSYGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSSYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GH FFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHHFFYLADEDGNALSLSRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+ FGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP ASHP SRD
Sbjct: 361 EKELEFDTRFEERFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDASHPGSRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAY ES
Sbjct: 841 SLVLLIMAEAYAES 853

BLAST of MS014956 vs. NCBI nr
Match: XP_023541215.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1578.9 bits (4087), Expect = 0.0e+00
Identity = 760/854 (88.99%), Postives = 807/854 (94.50%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSRIKSSSAVD   D AVRQ+PDIS RHRDR   R SIRIF+VNLKV+VGF
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPSIRIFDVNLKVLVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDL QFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLHQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GHLFFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHLFFYLADEDGNALSLSRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++ ETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIPETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGT FP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTDFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP  SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRD+LRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDKLRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. NCBI nr
Match: KAG6601592.1 (Mannosyl-oligosaccharide glucosidase GCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1578.5 bits (4086), Expect = 0.0e+00
Identity = 757/854 (88.64%), Postives = 807/854 (94.50%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSR KSSSAVD   D AVRQ PDIS RHRDR   R SIRIF+VNLK++VGF
Sbjct: 1   MTGHSRRNPRSRNKSSSAVDGDGDGAVRQVPDISRRHRDRNTLRPSIRIFDVNLKILVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLSRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTD+LK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDQLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP  SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSIS+L +KGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISDLIVKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLI+AEAYTES
Sbjct: 841 SLVLLIIAEAYTES 853

BLAST of MS014956 vs. ExPASy Swiss-Prot
Match: F4HTM3 (Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS1 PE=1 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 547/856 (63.90%), Postives = 659/856 (76.99%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M G SRR+ R RIKSSS +  G D+     P I    R + K   SI  F  NLK++VG 
Sbjct: 1   MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60

Query: 61  SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
             +   VI ++I  L+  G    + Q PRVITPFPAP+V+DL  FQGEHKESLYWGTYRP
Sbjct: 61  IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120

Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
           H+Y G+RARTP SL+AGLMWLGVK+  Y MRH C++SD+LS++GW  HN RD+G Q LV+
Sbjct: 121 HVYFGVRARTPLSLVAGLMWLGVKDEMYVMRHFCENSDDLSTFGWREHNGRDYGRQELVE 180

Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
            DM + TSF+K+K  G GYGGDW VRI+V +K     D++ RS HLFFYLADE GN L+L
Sbjct: 181 NDMVIETSFVKSKGDGLGYGGDWAVRIDVKNKGLN--DDVKRSAHLFFYLADEGGNVLNL 240

Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
             D +D   +SLL SGSR D+G+WQ+HLKS+  ++ HYSGFKT H +NLS+LVQ+NL  Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300

Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
           ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG     S  E+R+  LTG  L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360

Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
           D LK+K  EFD KF +CF L+ K D E+  VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420

Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
            SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480

Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
           IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+  ++   F A E  E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540

Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
            SFLERA VRL+AWFQWFNT+Q G+E  SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600

Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
           PSEDERHVDLRCWM LAADCMHSI+EL  K  ++    Y+     LSNF LLNQMH+D  
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660

Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
           YG Y DFGNHTEKV+L WKEV+ E G  +RQL+R+    P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720

Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
           IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 780

Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
           ILS+L HY+  DGPYRE++  I  ELRSNLIRNVVRNY+++G+IWEQY+Q  G GKG   
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 840

Query: 841 FTGWTSLVLLIMAEAY 852
           FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 849

BLAST of MS014956 vs. ExPASy Swiss-Prot
Match: Q84M89 (Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 1.7e-298
Identity = 484/773 (62.61%), Postives = 600/773 (77.62%), Query Frame = 0

Query: 80  DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWLGV 139
           + Q PRVITPFP+P++ DL  FQGEHKESLYWGTYRP +Y G+RARTP+SL+AGLMWL +
Sbjct: 17  ESQRPRVITPFPSPKLTDLSMFQGEHKESLYWGTYRPQMYFGVRARTPKSLVAGLMWLVM 76

Query: 140 KNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDW 199
           K+G+  MRH C++S++L S+GW  HN RDFG Q L +Q+M L TSF+K++E   GYGGDW
Sbjct: 77  KDGKPVMRHFCENSNDLKSFGWKEHNGRDFGRQELFEQNMVLETSFVKSEEGSLGYGGDW 136

Query: 200 TVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGN 259
           ++RI V +  ++  DE+ RS HLFFYLADE  N ++L  D + + E+S+LASGSR D+GN
Sbjct: 137 SIRINVQNMLND--DEVKRSTHLFFYLADEGCNGVNLGKDVLRLKESSVLASGSRQDVGN 196

Query: 260 WQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKFGRLQLSDTSDDSSNILVFQ 319
           WQ+HLKS+  ++ HY GFKT    NLS LVQ+NL  Q  K G LQLSD+S+DSSNI VFQ
Sbjct: 197 WQMHLKSQNHLETHYCGFKTPDIVNLSGLVQQNLTAQEEKSGLLQLSDSSEDSSNIYVFQ 256

Query: 320 ISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLKEKQLEFDTKFEKCFGLANK 379
           IS      ID+AF+SG     S  E R+  LTG  L+  L+EK + F+ KF +CF L++K
Sbjct: 257 ISTTNQSTIDIAFVSGIKEETSNMENRIMSLTGLPLSSLLEEKHIAFNAKFNECFNLSDK 316

Query: 380 FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRDSFLFYWPAELYTAVPCRPV 439
            D E+  VGKAA+ N+LGGIGYFYGQSKI  P +   +S D FL YWPAELYTAVP RP 
Sbjct: 317 LDPETLVVGKAAIGNMLGGIGYFYGQSKIHFPKSPLAKSEDDFLLYWPAELYTAVPSRPR 376

Query: 440 FPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEF 499
           FPRGFLWDEGFHQLLIW+ WD++I L+I+G+WLDLMNIDGWIPREQ+LGAEALSK+P+++
Sbjct: 377 FPRGFLWDEGFHQLLIWR-WDVHITLEIVGNWLDLMNIDGWIPREQVLGAEALSKIPKQY 436

Query: 500 IPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFLERAFVRLEAWFQWFNTTQP 559
           + Q PSN NPP L LV+R+L++G++   F   +  +I SFL+RAFVRL+AWF+WFNT+Q 
Sbjct: 437 VVQFPSNGNPPTLLLVIRDLINGIRTEKFNEADRDKILSFLDRAFVRLDAWFKWFNTSQI 496

Query: 560 GREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSEDERHVDLRCWMLLAADCMHS 619
           G+E+ SYYWHGRD+ T RELNPQ+L SGLDDYPRASHP+EDERHVDLRCWM LAAD M+S
Sbjct: 497 GKEKGSYYWHGRDNITNRELNPQSLSSGLDDYPRASHPNEDERHVDLRCWMYLAADSMNS 556

Query: 620 ISELSMKGK-EVGMVYSLAANTLSNFELLNQMHFDDAYGTYLDFGNHTEKVRLSWKEVMV 679
           I E   K    V   YS  A  LS+F LLNQMH+D  +G YLDFGNHTE+VRL WKEV+ 
Sbjct: 557 IQEFMGKNDILVTEDYSSIAKLLSDFTLLNQMHYDKDHGAYLDFGNHTEEVRLVWKEVIH 616

Query: 680 EQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSVFWT 739
           E G+ +R+L+RE    P LR+VPHIGYVS FP M RIIP +S IL KQLDLISN ++ W+
Sbjct: 617 EDGHLSRELVRETYGKPELRLVPHIGYVSFFPFMFRIIPADSSILDKQLDLISNGNIVWS 676

Query: 740 NYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALNHYATEDGPYRERALTIR 799
           +YGL SL+KTSSLYMKYN+EH APYWRG IWMNMNY+ILS+L+HY+T DGPY  +A TI 
Sbjct: 677 DYGLLSLAKTSSLYMKYNSEHQAPYWRGAIWMNMNYMILSSLHHYSTVDGPYNSKARTIY 736

Query: 800 DELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAY 852
           +ELRSNLIRNVVRNY Q+G IWE Y+Q  G G+GA  FTGW++L+LLIM+E Y
Sbjct: 737 EELRSNLIRNVVRNYDQTGIIWEHYDQTKGTGEGARVFTGWSALILLIMSEEY 786

BLAST of MS014956 vs. ExPASy Swiss-Prot
Match: Q80UM7 (Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 6.6e-154
Identity = 317/796 (39.82%), Postives = 446/796 (56.03%), Query Frame = 0

Query: 85  RVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--GVKNG 144
           R +T  PAP  +            L+WGTYRPH+Y G++ R+P+ LL GLMW   G   G
Sbjct: 68  RALTLHPAPSALPPDSSSPAVAPELFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127

Query: 145 R-YFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDWTV 204
               +RH C+  D +  YGW  H+ R FG Q + D  + L T F+  K  G  +GGDW+ 
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGRTFGRQHIHDGALRLTTEFV--KRPGGQHGGDWSW 187

Query: 205 RIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGNWQ 264
           R+ V  + S      L S  LFFY+   DG  + L          S+  SG  S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPEIGAKGQLKSI--SGHTSELGDFR 247

Query: 265 LHL----KSREDVKIH--YSGFKTAH--FHNLSELVQENL---------GTQARKF---- 324
           L L       + V  H  Y+ F +++     L+++V+  L         G    ++    
Sbjct: 248 LTLLPPTSPGDTVPKHGSYNVFWSSNPGLPQLTDMVKSRLNSWFQHRPPGASPDRYLGLP 307

Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRL 384
           G L+  +         L+ Q++ + PF ++  F SG+   G  +     RL GS LT  L
Sbjct: 308 GSLKWEERGPSGQGQFLIQQVTLKAPFSVEFVFESGSAATGGNQAS--GRLVGSQLTQAL 367

Query: 385 KEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRS 444
           +     F  +FEK F L  K    E   +G+ A++ LLGGIGYFYGQ  + LP  S   S
Sbjct: 368 ESHAAAFKERFEKTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTSMEGS 427

Query: 445 RDSF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 504
                   + P  L++ VP R  FPRGFLWDEGFHQL++ Q WD ++  + LGHWL L+N
Sbjct: 428 EQKMDPALFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVV-QRWDPHLTREALGHWLGLLN 487

Query: 505 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 564
            DGWI REQILG EA ++VP EF+ Q  ++ANPP L L +  ++ G            + 
Sbjct: 488 ADGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVVHMLEG---------HDPDD 547

Query: 565 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 624
            +FL +AF RL AWF W + +Q G    SY W GRD +    LNP+TL SGLDDYPRASH
Sbjct: 548 LAFLRKAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASH 607

Query: 625 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAY 684
           PS  ERH+DLRCW+ L A  +  ++E   + +    +  LAA +L     L+++H+    
Sbjct: 608 PSTAERHLDLRCWVALGARVLSQLAEQLGETEAAAELGPLAA-SLEEPGSLDELHWAPEL 667

Query: 685 GTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREV-LETPTLRMVPHIGYVSLFPLMGRI 744
           G + DFGNHT+ V+L  +     QG     L+R V    P L+ V  +GYVSLFPL+ ++
Sbjct: 668 GVFADFGNHTKAVQLKSRP---PQG-----LVRVVGRPPPRLQYVDALGYVSLFPLLLQL 727

Query: 745 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 804
           + P S  LG  LD++++    W+ +GLRSLS +S  Y + NTEHD PYWRG +W+N+NYL
Sbjct: 728 LDPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYL 787

Query: 805 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 852
            L AL+HY   +GP++ +A  +  ELR+N++RNV + Y  +GF+WEQY+ + G+G G  P
Sbjct: 788 ALGALHHYGHVEGPHKVQAAKLYHELRANVVRNVRQQYQATGFLWEQYSDQDGRGMGCRP 834

BLAST of MS014956 vs. ExPASy Swiss-Prot
Match: Q13724 (Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5)

HSP 1 Score: 542.0 bits (1395), Expect = 1.2e-152
Identity = 317/801 (39.58%), Postives = 439/801 (54.81%), Query Frame = 0

Query: 81  HQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--G 140
           ++  R +T   AP V+            L+WGTYRPH+Y G++ R+P+ LL GLMW   G
Sbjct: 66  YRARRAVTLHSAPPVLPADSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQG 125

Query: 141 VKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGD 200
              G   +RH C+  D +  YGW  H+   FG Q + D  + L T F+  K  G  +GGD
Sbjct: 126 TTPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIQDGALRLTTEFV--KRPGGQHGGD 185

Query: 201 WTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIG 260
           W+ R+ V  + S  +   L S  LFFY+   DG  + L    +         SG  S++G
Sbjct: 186 WSWRVTVEPQDSGTSALPLVS--LFFYVV-TDGKEVLLPE--VGAKGQLKFISGHTSELG 245

Query: 261 NWQLHL---KSREDVKIHYSGFKTAHFHN-----LSELVQENL---------GTQARKF- 320
           +++  L    S  D    Y  +      N     L+E+V+  L         G    ++ 
Sbjct: 246 DFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEMVKSRLNSWFQHRPPGAPPERYL 305

Query: 321 ---GRLQLSD---TSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSS 380
              G L+  D   +       L+ Q++ +IP  I+  F SG+   G    + + RL GS 
Sbjct: 306 GLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEFVFESGSAQAGG--NQALPRLAGSL 365

Query: 381 LTDRLKEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALP-- 440
           LT  L+     F  +FEK F L  K        +G+AA++ LLGGIGYFYGQ  + LP  
Sbjct: 366 LTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQAALSGLLGGIGYFYGQG-LVLPDI 425

Query: 441 GASHPRSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHW 500
           G      +     + P  L+TAVP R  FPRGFLWDEGFHQL++ Q WD  +  + LGHW
Sbjct: 426 GVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVV-QRWDPSLTREALGHW 485

Query: 501 LDLMNIDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAK 560
           L L+N DGWI REQILG EA ++VP EF+ Q   +ANPP L L +  ++           
Sbjct: 486 LGLLNADGWIGREQILGDEARARVPPEFLVQRAVHANPPTLLLPVAHMLE---------V 545

Query: 561 ESSEISSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDY 620
              +  +FL +A  RL AWF W + +Q G    SY W GRD +    LNP+TL SGLDDY
Sbjct: 546 GDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRDPALPTLLNPKTLPSGLDDY 605

Query: 621 PRASHPSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMH 680
           PRASHPS  ERH+DLRCW+ L A  +  ++E  +   EV       A +L   E L+++H
Sbjct: 606 PRASHPSVTERHLDLRCWVALGARVLTRLAE-HLGEAEVAAELGPLAASLEAAESLDELH 665

Query: 681 FDDAYGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREV-LETPTLRMVPHIGYVSLFP 740
           +    G + DFGNHT+ V+L  +     QG     L+R V    P L+ V  +GYVSLFP
Sbjct: 666 WAPELGVFADFGNHTKAVQLKPRP---PQG-----LVRVVGRPQPQLQYVDALGYVSLFP 725

Query: 741 LMGRIIPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWM 800
           L+ R++ P S  LG  LD++++    W+ +GLRSL+ +SS Y + N+EHD PYWRG +W+
Sbjct: 726 LLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWL 785

Query: 801 NMNYLILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKG 852
           N+NYL L AL+HY   +GP++ RA  +  ELR+N++ NV R Y  +GF+WEQY+ + G+G
Sbjct: 786 NVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRG 837

BLAST of MS014956 vs. ExPASy Swiss-Prot
Match: O88941 (Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 5.2e-151
Identity = 313/796 (39.32%), Postives = 444/796 (55.78%), Query Frame = 0

Query: 85  RVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--GVKNG 144
           R +T  PAP  +             +WGTYRPH+Y G++ R+P+ LL GLMW   G   G
Sbjct: 68  RALTLHPAPSALPPDSSSPAVAPEFFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127

Query: 145 R-YFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDWTV 204
               +RH C+  D +  YGW  H+   FG Q + D  + L T F+  K SG  +GGDW+ 
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIYDGALRLTTEFV--KRSGGHHGGDWSW 187

Query: 205 RIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGNWQ 264
           R+ V  + S      L S  LFFY+   DG  + L    +         SG  S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPE--VGAKGQLKFISGHTSELGDFR 247

Query: 265 LHL----KSREDVKIH--YSGFKTAH--FHNLSELVQENL---------GTQARKF---- 324
           L L       + V  H  Y+ F +++     L+++V+ +L         G    ++    
Sbjct: 248 LTLLPPTTPGDTVPKHGSYNVFWSSNPGLPLLTDMVKSHLNSWFHHRPPGASPERYLGLP 307

Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRL 384
           G L+  +         LV Q++ + PF ++  F SG+   G   ++   +L G  LT  L
Sbjct: 308 GSLKWEERGPSGQGQFLVQQVTLKAPFSVEFVFESGSARTG--RDQASEQLVGGQLTRAL 367

Query: 385 KEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRS 444
           +     F  +FE+ F L  K    E   +G+ A++ LLGGIGYFYGQ  + LP      S
Sbjct: 368 ESHAAAFKERFERTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTGMEGS 427

Query: 445 RDSF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 504
                   + P  L++ VP R  FPRGFLWDEGFHQL++ Q WD ++  + LGHWL L+N
Sbjct: 428 EQKMDPSLFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVV-QRWDPHLTREALGHWLGLLN 487

Query: 505 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 564
            DGWI REQILG EA ++VP EF+ Q  ++ANPP L L +  ++ G          + E 
Sbjct: 488 ADGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVIHMLEG---------RAPED 547

Query: 565 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 624
            +FL RAF RL AWF W + +Q G    SY W GRD +    LNP+TL SGLDDYPRASH
Sbjct: 548 LAFLRRAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASH 607

Query: 625 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAY 684
           PS  ERH+DLRCW+ L A  +  ++E   + +    +  LAA +L     L+++H+    
Sbjct: 608 PSAAERHLDLRCWVTLGARVLSQLAEELGETEAAAELGPLAA-SLEAAGSLDELHWAPEL 667

Query: 685 GTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPT-LRMVPHIGYVSLFPLMGRI 744
           G + DFGNHT+ V+L  +     QG     L+R V   P  L+ V  +GYVSLFPL+ ++
Sbjct: 668 GVFADFGNHTKAVQLKSRP---PQG-----LVRVVGRPPARLQYVDALGYVSLFPLLLQL 727

Query: 745 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 804
           + P S  LG  LD++++    W+ +GLRSLS +S  Y + NTEHD PYWRG +W+N+NYL
Sbjct: 728 LEPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYL 787

Query: 805 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 852
            L AL+HY   +GP++ +A  +  ELR+N++ NV + Y  +GF+WEQY+ + G+G G  P
Sbjct: 788 ALGALHHYGRVEGPHKVQAAKLYRELRANVVSNVRQQYQATGFLWEQYSDQDGRGMGCRP 834

BLAST of MS014956 vs. ExPASy TrEMBL
Match: A0A6J1DD68 (mannosyl-oligosaccharide glucosidase GCS1-like OS=Momordica charantia OX=3673 GN=LOC111019700 PE=3 SV=1)

HSP 1 Score: 1735.7 bits (4494), Expect = 0.0e+00
Identity = 844/854 (98.83%), Postives = 850/854 (99.53%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF
Sbjct: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL
Sbjct: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           G+RARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT
Sbjct: 121 GMRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLR+GHLFFYLADEDGNSLSLSSDA
Sbjct: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRTGHLFFYLADEDGNSLSLSSDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF
Sbjct: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFKIDM FISGTGFPGSREEERVNRLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMTFISGTGFPGSREEERVNRLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD
Sbjct: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDIYICLDILGHWLDLMNIDGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIYICLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESS+ISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSKISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+EESSYYWHGRDSS IRELNPQTLMSGLDDYPRASHPSED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEESSYYWHGRDSSPIRELNPQTLMSGLDDYPRASHPSED 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRL WKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. ExPASy TrEMBL
Match: A0A6J1H118 (mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC111458598 PE=3 SV=1)

HSP 1 Score: 1584.7 bits (4102), Expect = 0.0e+00
Identity = 762/854 (89.23%), Postives = 807/854 (94.50%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSRIKSSSAVD   D AVRQ+PDIS RHRDR   R  IRIF+VNLKV+VGF
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPLIRIFDVNLKVLVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFGAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP  SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQHPSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQHPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL MKGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIMKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPQLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. ExPASy TrEMBL
Match: A0A6J1KB47 (mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita maxima OX=3661 GN=LOC111492274 PE=3 SV=1)

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 764/854 (89.46%), Postives = 808/854 (94.61%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M GHSRRNPRSRIKSSSAVD G D AVRQ+PDIS RHRDR   R  IRIF+VNLKV+VGF
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGGGDGAVRQAPDISRRHRDRNTLRPWIRIFDVNLKVLVGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
           SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61  SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELSSYGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSSYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GH FFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHHFFYLADEDGNALSLSRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+ FGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP ASHP SRD
Sbjct: 361 EKELEFDTRFEERFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDASHPGSRD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNIDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV  TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATEDGPYRERA  IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAY ES
Sbjct: 841 SLVLLIMAEAYAES 853

BLAST of MS014956 vs. ExPASy TrEMBL
Match: A0A5A7SUQ8 (Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001990 PE=3 SV=1)

HSP 1 Score: 1534.2 bits (3971), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 795/854 (93.09%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M G SRRNPRSRIKS+ AVD   D AVRQ+ D   RHRD  KSR SIRIFNVNLKVM+GF
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
            F+AFFVILYLI SL+  GDHQTPRVITPFPA +V DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWT HNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLK+KESGSGYGGDW VRI+VNSKKSEEA+E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           +++HETSLLASGSRSD+GNWQLHL+SR+++K+H+SGFKT H+HNLSELVQEN+G QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISG IPF+ D+AFISG+G P SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+ FGLANKFD ESTTVGKAAV+NLLGGIGYFYG+SKIALPGASHP S+D
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMN+DGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNVDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEALSKVPEEFIPQ+PSNANPPALFLV+REL+HGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP+ED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTED 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL+ KGK +  VYS A   LS FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRL WKEVM  QG+TTRQLIREV E P LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WILGKQLDLISNRS+FWT+YGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATE GPYRERA  IRDELRSN+IRNVVRNYH+SG++WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. ExPASy TrEMBL
Match: A0A1S3BE51 (mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103488898 PE=3 SV=1)

HSP 1 Score: 1534.2 bits (3971), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 795/854 (93.09%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M G SRRNPRSRIKS+ AVD   D AVRQ+ D   RHRD  KSR SIRIFNVNLKVM+GF
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
            F+AFFVILYLI SL+  GDHQTPRVITPFPA +V DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWT HNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
           L TSFLK+KESGSGYGGDW VRI+VNSKKSEEA+E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
           +++HETSLLASGSRSD+GNWQLHL+SR+++K+H+SGFKT H+HNLSELVQEN+G QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISG IPF+ D+AFISG+G P SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360

Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
           EK+LEFDT+FE+ FGLANKFD ESTTVGKAAV+NLLGGIGYFYG+SKIALPGASHP S+D
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420

Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
           SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMN+DGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNVDGW 480

Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
           IPREQILGAEALSKVPEEFIPQ+PSNANPPALFLV+REL+HGLK+NAFT  ESSEISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFL 540

Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
           ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP+ED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTED 600

Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
           ERHVDLRCWMLLAADCMHSISEL+ KGK +  VYS A   LS FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYL 660

Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
           DFGNHTEKVRL WKEVM  QG+TTRQLIREV E P LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPES 720

Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
           WILGKQLDLISNRS+FWT+YGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780

Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
           NHYATE GPYRERA  IRDELRSN+IRNVVRNYH+SG++WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWT 840

Query: 841 SLVLLIMAEAYTES 855
           SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853

BLAST of MS014956 vs. TAIR 10
Match: AT1G67490.1 (glucosidase 1 )

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 547/856 (63.90%), Postives = 659/856 (76.99%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M G SRR+ R RIKSSS +  G D+     P I    R + K   SI  F  NLK++VG 
Sbjct: 1   MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60

Query: 61  SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
             +   VI ++I  L+  G    + Q PRVITPFPAP+V+DL  FQGEHKESLYWGTYRP
Sbjct: 61  IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120

Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
           H+Y G+RARTP SL+AGLMWLGVK+  Y MRH C++SD+LS++GW  HN RD+G Q LV+
Sbjct: 121 HVYFGVRARTPLSLVAGLMWLGVKDEMYVMRHFCENSDDLSTFGWREHNGRDYGRQELVE 180

Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
            DM + TSF+K+K  G GYGGDW VRI+V +K     D++ RS HLFFYLADE GN L+L
Sbjct: 181 NDMVIETSFVKSKGDGLGYGGDWAVRIDVKNKGLN--DDVKRSAHLFFYLADEGGNVLNL 240

Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
             D +D   +SLL SGSR D+G+WQ+HLKS+  ++ HYSGFKT H +NLS+LVQ+NL  Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300

Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
           ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG     S  E+R+  LTG  L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360

Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
           D LK+K  EFD KF +CF L+ K D E+  VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420

Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
            SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480

Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
           IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+  ++   F A E  E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540

Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
            SFLERA VRL+AWFQWFNT+Q G+E  SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600

Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
           PSEDERHVDLRCWM LAADCMHSI+EL  K  ++    Y+     LSNF LLNQMH+D  
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660

Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
           YG Y DFGNHTEKV+L WKEV+ E G  +RQL+R+    P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720

Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
           IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 780

Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
           ILS+L HY+  DGPYRE++  I  ELRSNLIRNVVRNY+++G+IWEQY+Q  G GKG   
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 840

Query: 841 FTGWTSLVLLIMAEAY 852
           FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 849

BLAST of MS014956 vs. TAIR 10
Match: AT1G24320.1 (Six-hairpin glycosidases superfamily protein )

HSP 1 Score: 1026.5 bits (2653), Expect = 1.2e-299
Identity = 484/773 (62.61%), Postives = 600/773 (77.62%), Query Frame = 0

Query: 80  DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWLGV 139
           + Q PRVITPFP+P++ DL  FQGEHKESLYWGTYRP +Y G+RARTP+SL+AGLMWL +
Sbjct: 17  ESQRPRVITPFPSPKLTDLSMFQGEHKESLYWGTYRPQMYFGVRARTPKSLVAGLMWLVM 76

Query: 140 KNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDW 199
           K+G+  MRH C++S++L S+GW  HN RDFG Q L +Q+M L TSF+K++E   GYGGDW
Sbjct: 77  KDGKPVMRHFCENSNDLKSFGWKEHNGRDFGRQELFEQNMVLETSFVKSEEGSLGYGGDW 136

Query: 200 TVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGN 259
           ++RI V +  ++  DE+ RS HLFFYLADE  N ++L  D + + E+S+LASGSR D+GN
Sbjct: 137 SIRINVQNMLND--DEVKRSTHLFFYLADEGCNGVNLGKDVLRLKESSVLASGSRQDVGN 196

Query: 260 WQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKFGRLQLSDTSDDSSNILVFQ 319
           WQ+HLKS+  ++ HY GFKT    NLS LVQ+NL  Q  K G LQLSD+S+DSSNI VFQ
Sbjct: 197 WQMHLKSQNHLETHYCGFKTPDIVNLSGLVQQNLTAQEEKSGLLQLSDSSEDSSNIYVFQ 256

Query: 320 ISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLKEKQLEFDTKFEKCFGLANK 379
           IS      ID+AF+SG     S  E R+  LTG  L+  L+EK + F+ KF +CF L++K
Sbjct: 257 ISTTNQSTIDIAFVSGIKEETSNMENRIMSLTGLPLSSLLEEKHIAFNAKFNECFNLSDK 316

Query: 380 FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRDSFLFYWPAELYTAVPCRPV 439
            D E+  VGKAA+ N+LGGIGYFYGQSKI  P +   +S D FL YWPAELYTAVP RP 
Sbjct: 317 LDPETLVVGKAAIGNMLGGIGYFYGQSKIHFPKSPLAKSEDDFLLYWPAELYTAVPSRPR 376

Query: 440 FPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEF 499
           FPRGFLWDEGFHQLLIW+ WD++I L+I+G+WLDLMNIDGWIPREQ+LGAEALSK+P+++
Sbjct: 377 FPRGFLWDEGFHQLLIWR-WDVHITLEIVGNWLDLMNIDGWIPREQVLGAEALSKIPKQY 436

Query: 500 IPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFLERAFVRLEAWFQWFNTTQP 559
           + Q PSN NPP L LV+R+L++G++   F   +  +I SFL+RAFVRL+AWF+WFNT+Q 
Sbjct: 437 VVQFPSNGNPPTLLLVIRDLINGIRTEKFNEADRDKILSFLDRAFVRLDAWFKWFNTSQI 496

Query: 560 GREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSEDERHVDLRCWMLLAADCMHS 619
           G+E+ SYYWHGRD+ T RELNPQ+L SGLDDYPRASHP+EDERHVDLRCWM LAAD M+S
Sbjct: 497 GKEKGSYYWHGRDNITNRELNPQSLSSGLDDYPRASHPNEDERHVDLRCWMYLAADSMNS 556

Query: 620 ISELSMKGK-EVGMVYSLAANTLSNFELLNQMHFDDAYGTYLDFGNHTEKVRLSWKEVMV 679
           I E   K    V   YS  A  LS+F LLNQMH+D  +G YLDFGNHTE+VRL WKEV+ 
Sbjct: 557 IQEFMGKNDILVTEDYSSIAKLLSDFTLLNQMHYDKDHGAYLDFGNHTEEVRLVWKEVIH 616

Query: 680 EQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSVFWT 739
           E G+ +R+L+RE    P LR+VPHIGYVS FP M RIIP +S IL KQLDLISN ++ W+
Sbjct: 617 EDGHLSRELVRETYGKPELRLVPHIGYVSFFPFMFRIIPADSSILDKQLDLISNGNIVWS 676

Query: 740 NYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALNHYATEDGPYRERALTIR 799
           +YGL SL+KTSSLYMKYN+EH APYWRG IWMNMNY+ILS+L+HY+T DGPY  +A TI 
Sbjct: 677 DYGLLSLAKTSSLYMKYNSEHQAPYWRGAIWMNMNYMILSSLHHYSTVDGPYNSKARTIY 736

Query: 800 DELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAY 852
           +ELRSNLIRNVVRNY Q+G IWE Y+Q  G G+GA  FTGW++L+LLIM+E Y
Sbjct: 737 EELRSNLIRNVVRNYDQTGIIWEHYDQTKGTGEGARVFTGWSALILLIMSEEY 786

BLAST of MS014956 vs. TAIR 10
Match: AT1G67490.2 (glucosidase 1 )

HSP 1 Score: 986.9 bits (2550), Expect = 1.0e-287
Identity = 496/856 (57.94%), Postives = 594/856 (69.39%), Query Frame = 0

Query: 1   MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
           M G SRR+ R RIKSSS +  G D+     P I    R + K   SI  F  NLK++VG 
Sbjct: 1   MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60

Query: 61  SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
             +   VI ++I  L+  G    + Q PRVITPFPAP+V+DL  FQGEHKESLYWGTYRP
Sbjct: 61  IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120

Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
           H+Y G+RAR                                                   
Sbjct: 121 HVYFGVRARL-------------------------------------------------- 180

Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
                                                D++ RS HLFFYLADE GN L+L
Sbjct: 181 ------------------------------------NDDVKRSAHLFFYLADEGGNVLNL 240

Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
             D +D   +SLL SGSR D+G+WQ+HLKS+  ++ HYSGFKT H +NLS+LVQ+NL  Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300

Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
           ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG     S  E+R+  LTG  L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360

Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
           D LK+K  EFD KF +CF L+ K D E+  VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420

Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
            SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480

Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
           IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+  ++   F A E  E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540

Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
            SFLERA VRL+AWFQWFNT+Q G+E  SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600

Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
           PSEDERHVDLRCWM LAADCMHSI+EL  K  ++    Y+     LSNF LLNQMH+D  
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660

Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
           YG Y DFGNHTEKV+L WKEV+ E G  +RQL+R+    P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720

Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
           IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 765

Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
           ILS+L HY+  DGPYRE++  I  ELRSNLIRNVVRNY+++G+IWEQY+Q  G GKG   
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 765

Query: 841 FTGWTSLVLLIMAEAY 852
           FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 765

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151803.10.0e+0098.83mannosyl-oligosaccharide glucosidase GCS1-like [Momordica charantia][more]
XP_022957119.10.0e+0089.23mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita moschata][more]
XP_022997334.10.0e+0089.46mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita maxima][more]
XP_023541215.10.0e+0088.99mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita pepo subsp. pepo][more]
KAG6601592.10.0e+0088.64Mannosyl-oligosaccharide glucosidase GCS1, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
F4HTM30.0e+0063.90Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS... [more]
Q84M891.7e-29862.61Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1[more]
Q80UM76.6e-15439.82Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1[more]
Q137241.2e-15239.58Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5[more]
O889415.2e-15139.32Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DD680.0e+0098.83mannosyl-oligosaccharide glucosidase GCS1-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1H1180.0e+0089.23mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1KB470.0e+0089.46mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A5A7SUQ80.0e+0086.07Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BE510.0e+0086.07mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
AT1G67490.10.0e+0063.90glucosidase 1 [more]
AT1G24320.11.2e-29962.61Six-hairpin glycosidases superfamily protein [more]
AT1G67490.21.0e-28757.94glucosidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 379..854
e-value: 5.9E-158
score: 528.5
IPR031631Glycosyl hydrolase family 63, N-terminalPFAMPF16923Glyco_hydro_63Ncoord: 108..273
e-value: 3.6E-42
score: 145.0
IPR038518Glycosyl hydrolase family 63, N-terminal domain superfamilyGENE3D2.70.98.110coord: 96..378
e-value: 1.5E-75
score: 256.8
IPR031335Glycosyl hydrolase family 63, C-terminalPFAMPF03200Glyco_hydro_63coord: 350..849
e-value: 9.6E-213
score: 707.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..39
NoneNo IPR availablePANTHERPTHR10412:SF17ALPHA-GLUCOSIDASE 2coord: 80..851
IPR004888Glycoside hydrolase family 63PANTHERPTHR10412MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASEcoord: 80..851
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 368..851

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014956.1MS014956.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009311 oligosaccharide metabolic process
molecular_function GO:0004573 Glc3Man9GlcNAc2 oligosaccharide glucosidase activity