Homology
BLAST of MS014956 vs. NCBI nr
Match:
XP_022151803.1 (mannosyl-oligosaccharide glucosidase GCS1-like [Momordica charantia])
HSP 1 Score: 1735.7 bits (4494), Expect = 0.0e+00
Identity = 844/854 (98.83%), Postives = 850/854 (99.53%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF
Sbjct: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL
Sbjct: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
G+RARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT
Sbjct: 121 GMRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLR+GHLFFYLADEDGNSLSLSSDA
Sbjct: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRTGHLFFYLADEDGNSLSLSSDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF
Sbjct: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPFKIDM FISGTGFPGSREEERVNRLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMTFISGTGFPGSREEERVNRLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD
Sbjct: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDIYICLDILGHWLDLMNIDGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIYICLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESS+ISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSKISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+EESSYYWHGRDSS IRELNPQTLMSGLDDYPRASHPSED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEESSYYWHGRDSSPIRELNPQTLMSGLDDYPRASHPSED 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRL WKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. NCBI nr
Match:
XP_022957119.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita moschata])
HSP 1 Score: 1584.7 bits (4102), Expect = 0.0e+00
Identity = 762/854 (89.23%), Postives = 807/854 (94.50%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSRIKSSSAVD D AVRQ+PDIS RHRDR R IRIF+VNLKV+VGF
Sbjct: 1 MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPLIRIFDVNLKVLVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFGAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLNRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQHPSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQHPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL MKGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIMKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPQLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. NCBI nr
Match:
XP_022997334.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita maxima])
HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 764/854 (89.46%), Postives = 808/854 (94.61%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSRIKSSSAVD G D AVRQ+PDIS RHRDR R IRIF+VNLKV+VGF
Sbjct: 1 MTGHSRRNPRSRIKSSSAVDGGGDGAVRQAPDISRRHRDRNTLRPWIRIFDVNLKVLVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELSSYGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSSYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GH FFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHHFFYLADEDGNALSLSRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+ FGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP ASHP SRD
Sbjct: 361 EKELEFDTRFEERFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDASHPGSRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAY ES
Sbjct: 841 SLVLLIMAEAYAES 853
BLAST of MS014956 vs. NCBI nr
Match:
XP_023541215.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1578.9 bits (4087), Expect = 0.0e+00
Identity = 760/854 (88.99%), Postives = 807/854 (94.50%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSRIKSSSAVD D AVRQ+PDIS RHRDR R SIRIF+VNLKV+VGF
Sbjct: 1 MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPSIRIFDVNLKVLVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDL QFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLHQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GHLFFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHLFFYLADEDGNALSLSRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++ ETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIPETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGT FP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTDFPSSREEERVNSLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRD+LRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDKLRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. NCBI nr
Match:
KAG6601592.1 (Mannosyl-oligosaccharide glucosidase GCS1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1578.5 bits (4086), Expect = 0.0e+00
Identity = 757/854 (88.64%), Postives = 807/854 (94.50%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSR KSSSAVD D AVRQ PDIS RHRDR R SIRIF+VNLK++VGF
Sbjct: 1 MTGHSRRNPRSRNKSSSAVDGDGDGAVRQVPDISRRHRDRNTLRPSIRIFDVNLKILVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLSRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTD+LK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDQLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSIS+L +KGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISDLIVKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLI+AEAYTES
Sbjct: 841 SLVLLIIAEAYTES 853
BLAST of MS014956 vs. ExPASy Swiss-Prot
Match:
F4HTM3 (Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS1 PE=1 SV=1)
HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 547/856 (63.90%), Postives = 659/856 (76.99%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M G SRR+ R RIKSSS + G D+ P I R + K SI F NLK++VG
Sbjct: 1 MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60
Query: 61 SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
+ VI ++I L+ G + Q PRVITPFPAP+V+DL FQGEHKESLYWGTYRP
Sbjct: 61 IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120
Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
H+Y G+RARTP SL+AGLMWLGVK+ Y MRH C++SD+LS++GW HN RD+G Q LV+
Sbjct: 121 HVYFGVRARTPLSLVAGLMWLGVKDEMYVMRHFCENSDDLSTFGWREHNGRDYGRQELVE 180
Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
DM + TSF+K+K G GYGGDW VRI+V +K D++ RS HLFFYLADE GN L+L
Sbjct: 181 NDMVIETSFVKSKGDGLGYGGDWAVRIDVKNKGLN--DDVKRSAHLFFYLADEGGNVLNL 240
Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
D +D +SLL SGSR D+G+WQ+HLKS+ ++ HYSGFKT H +NLS+LVQ+NL Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300
Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG S E+R+ LTG L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360
Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
D LK+K EFD KF +CF L+ K D E+ VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420
Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480
Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+ ++ F A E E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540
Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
SFLERA VRL+AWFQWFNT+Q G+E SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600
Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
PSEDERHVDLRCWM LAADCMHSI+EL K ++ Y+ LSNF LLNQMH+D
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660
Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
YG Y DFGNHTEKV+L WKEV+ E G +RQL+R+ P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720
Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 780
Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
ILS+L HY+ DGPYRE++ I ELRSNLIRNVVRNY+++G+IWEQY+Q G GKG
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 840
Query: 841 FTGWTSLVLLIMAEAY 852
FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 849
BLAST of MS014956 vs. ExPASy Swiss-Prot
Match:
Q84M89 (Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1)
HSP 1 Score: 1026.5 bits (2653), Expect = 1.7e-298
Identity = 484/773 (62.61%), Postives = 600/773 (77.62%), Query Frame = 0
Query: 80 DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWLGV 139
+ Q PRVITPFP+P++ DL FQGEHKESLYWGTYRP +Y G+RARTP+SL+AGLMWL +
Sbjct: 17 ESQRPRVITPFPSPKLTDLSMFQGEHKESLYWGTYRPQMYFGVRARTPKSLVAGLMWLVM 76
Query: 140 KNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDW 199
K+G+ MRH C++S++L S+GW HN RDFG Q L +Q+M L TSF+K++E GYGGDW
Sbjct: 77 KDGKPVMRHFCENSNDLKSFGWKEHNGRDFGRQELFEQNMVLETSFVKSEEGSLGYGGDW 136
Query: 200 TVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGN 259
++RI V + ++ DE+ RS HLFFYLADE N ++L D + + E+S+LASGSR D+GN
Sbjct: 137 SIRINVQNMLND--DEVKRSTHLFFYLADEGCNGVNLGKDVLRLKESSVLASGSRQDVGN 196
Query: 260 WQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKFGRLQLSDTSDDSSNILVFQ 319
WQ+HLKS+ ++ HY GFKT NLS LVQ+NL Q K G LQLSD+S+DSSNI VFQ
Sbjct: 197 WQMHLKSQNHLETHYCGFKTPDIVNLSGLVQQNLTAQEEKSGLLQLSDSSEDSSNIYVFQ 256
Query: 320 ISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLKEKQLEFDTKFEKCFGLANK 379
IS ID+AF+SG S E R+ LTG L+ L+EK + F+ KF +CF L++K
Sbjct: 257 ISTTNQSTIDIAFVSGIKEETSNMENRIMSLTGLPLSSLLEEKHIAFNAKFNECFNLSDK 316
Query: 380 FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRDSFLFYWPAELYTAVPCRPV 439
D E+ VGKAA+ N+LGGIGYFYGQSKI P + +S D FL YWPAELYTAVP RP
Sbjct: 317 LDPETLVVGKAAIGNMLGGIGYFYGQSKIHFPKSPLAKSEDDFLLYWPAELYTAVPSRPR 376
Query: 440 FPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEF 499
FPRGFLWDEGFHQLLIW+ WD++I L+I+G+WLDLMNIDGWIPREQ+LGAEALSK+P+++
Sbjct: 377 FPRGFLWDEGFHQLLIWR-WDVHITLEIVGNWLDLMNIDGWIPREQVLGAEALSKIPKQY 436
Query: 500 IPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFLERAFVRLEAWFQWFNTTQP 559
+ Q PSN NPP L LV+R+L++G++ F + +I SFL+RAFVRL+AWF+WFNT+Q
Sbjct: 437 VVQFPSNGNPPTLLLVIRDLINGIRTEKFNEADRDKILSFLDRAFVRLDAWFKWFNTSQI 496
Query: 560 GREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSEDERHVDLRCWMLLAADCMHS 619
G+E+ SYYWHGRD+ T RELNPQ+L SGLDDYPRASHP+EDERHVDLRCWM LAAD M+S
Sbjct: 497 GKEKGSYYWHGRDNITNRELNPQSLSSGLDDYPRASHPNEDERHVDLRCWMYLAADSMNS 556
Query: 620 ISELSMKGK-EVGMVYSLAANTLSNFELLNQMHFDDAYGTYLDFGNHTEKVRLSWKEVMV 679
I E K V YS A LS+F LLNQMH+D +G YLDFGNHTE+VRL WKEV+
Sbjct: 557 IQEFMGKNDILVTEDYSSIAKLLSDFTLLNQMHYDKDHGAYLDFGNHTEEVRLVWKEVIH 616
Query: 680 EQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSVFWT 739
E G+ +R+L+RE P LR+VPHIGYVS FP M RIIP +S IL KQLDLISN ++ W+
Sbjct: 617 EDGHLSRELVRETYGKPELRLVPHIGYVSFFPFMFRIIPADSSILDKQLDLISNGNIVWS 676
Query: 740 NYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALNHYATEDGPYRERALTIR 799
+YGL SL+KTSSLYMKYN+EH APYWRG IWMNMNY+ILS+L+HY+T DGPY +A TI
Sbjct: 677 DYGLLSLAKTSSLYMKYNSEHQAPYWRGAIWMNMNYMILSSLHHYSTVDGPYNSKARTIY 736
Query: 800 DELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAY 852
+ELRSNLIRNVVRNY Q+G IWE Y+Q G G+GA FTGW++L+LLIM+E Y
Sbjct: 737 EELRSNLIRNVVRNYDQTGIIWEHYDQTKGTGEGARVFTGWSALILLIMSEEY 786
BLAST of MS014956 vs. ExPASy Swiss-Prot
Match:
Q80UM7 (Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1)
HSP 1 Score: 546.2 bits (1406), Expect = 6.6e-154
Identity = 317/796 (39.82%), Postives = 446/796 (56.03%), Query Frame = 0
Query: 85 RVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--GVKNG 144
R +T PAP + L+WGTYRPH+Y G++ R+P+ LL GLMW G G
Sbjct: 68 RALTLHPAPSALPPDSSSPAVAPELFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127
Query: 145 R-YFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDWTV 204
+RH C+ D + YGW H+ R FG Q + D + L T F+ K G +GGDW+
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGRTFGRQHIHDGALRLTTEFV--KRPGGQHGGDWSW 187
Query: 205 RIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGNWQ 264
R+ V + S L S LFFY+ DG + L S+ SG S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPEIGAKGQLKSI--SGHTSELGDFR 247
Query: 265 LHL----KSREDVKIH--YSGFKTAH--FHNLSELVQENL---------GTQARKF---- 324
L L + V H Y+ F +++ L+++V+ L G ++
Sbjct: 248 LTLLPPTSPGDTVPKHGSYNVFWSSNPGLPQLTDMVKSRLNSWFQHRPPGASPDRYLGLP 307
Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRL 384
G L+ + L+ Q++ + PF ++ F SG+ G + RL GS LT L
Sbjct: 308 GSLKWEERGPSGQGQFLIQQVTLKAPFSVEFVFESGSAATGGNQAS--GRLVGSQLTQAL 367
Query: 385 KEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRS 444
+ F +FEK F L K E +G+ A++ LLGGIGYFYGQ + LP S S
Sbjct: 368 ESHAAAFKERFEKTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTSMEGS 427
Query: 445 RDSF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 504
+ P L++ VP R FPRGFLWDEGFHQL++ Q WD ++ + LGHWL L+N
Sbjct: 428 EQKMDPALFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVV-QRWDPHLTREALGHWLGLLN 487
Query: 505 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 564
DGWI REQILG EA ++VP EF+ Q ++ANPP L L + ++ G +
Sbjct: 488 ADGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVVHMLEG---------HDPDD 547
Query: 565 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 624
+FL +AF RL AWF W + +Q G SY W GRD + LNP+TL SGLDDYPRASH
Sbjct: 548 LAFLRKAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASH 607
Query: 625 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAY 684
PS ERH+DLRCW+ L A + ++E + + + LAA +L L+++H+
Sbjct: 608 PSTAERHLDLRCWVALGARVLSQLAEQLGETEAAAELGPLAA-SLEEPGSLDELHWAPEL 667
Query: 685 GTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREV-LETPTLRMVPHIGYVSLFPLMGRI 744
G + DFGNHT+ V+L + QG L+R V P L+ V +GYVSLFPL+ ++
Sbjct: 668 GVFADFGNHTKAVQLKSRP---PQG-----LVRVVGRPPPRLQYVDALGYVSLFPLLLQL 727
Query: 745 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 804
+ P S LG LD++++ W+ +GLRSLS +S Y + NTEHD PYWRG +W+N+NYL
Sbjct: 728 LDPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYL 787
Query: 805 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 852
L AL+HY +GP++ +A + ELR+N++RNV + Y +GF+WEQY+ + G+G G P
Sbjct: 788 ALGALHHYGHVEGPHKVQAAKLYHELRANVVRNVRQQYQATGFLWEQYSDQDGRGMGCRP 834
BLAST of MS014956 vs. ExPASy Swiss-Prot
Match:
Q13724 (Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5)
HSP 1 Score: 542.0 bits (1395), Expect = 1.2e-152
Identity = 317/801 (39.58%), Postives = 439/801 (54.81%), Query Frame = 0
Query: 81 HQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--G 140
++ R +T AP V+ L+WGTYRPH+Y G++ R+P+ LL GLMW G
Sbjct: 66 YRARRAVTLHSAPPVLPADSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQG 125
Query: 141 VKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGD 200
G +RH C+ D + YGW H+ FG Q + D + L T F+ K G +GGD
Sbjct: 126 TTPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIQDGALRLTTEFV--KRPGGQHGGD 185
Query: 201 WTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIG 260
W+ R+ V + S + L S LFFY+ DG + L + SG S++G
Sbjct: 186 WSWRVTVEPQDSGTSALPLVS--LFFYVV-TDGKEVLLPE--VGAKGQLKFISGHTSELG 245
Query: 261 NWQLHL---KSREDVKIHYSGFKTAHFHN-----LSELVQENL---------GTQARKF- 320
+++ L S D Y + N L+E+V+ L G ++
Sbjct: 246 DFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEMVKSRLNSWFQHRPPGAPPERYL 305
Query: 321 ---GRLQLSD---TSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSS 380
G L+ D + L+ Q++ +IP I+ F SG+ G + + RL GS
Sbjct: 306 GLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEFVFESGSAQAGG--NQALPRLAGSL 365
Query: 381 LTDRLKEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALP-- 440
LT L+ F +FEK F L K +G+AA++ LLGGIGYFYGQ + LP
Sbjct: 366 LTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQAALSGLLGGIGYFYGQG-LVLPDI 425
Query: 441 GASHPRSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHW 500
G + + P L+TAVP R FPRGFLWDEGFHQL++ Q WD + + LGHW
Sbjct: 426 GVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVV-QRWDPSLTREALGHW 485
Query: 501 LDLMNIDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAK 560
L L+N DGWI REQILG EA ++VP EF+ Q +ANPP L L + ++
Sbjct: 486 LGLLNADGWIGREQILGDEARARVPPEFLVQRAVHANPPTLLLPVAHMLE---------V 545
Query: 561 ESSEISSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDY 620
+ +FL +A RL AWF W + +Q G SY W GRD + LNP+TL SGLDDY
Sbjct: 546 GDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRDPALPTLLNPKTLPSGLDDY 605
Query: 621 PRASHPSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMH 680
PRASHPS ERH+DLRCW+ L A + ++E + EV A +L E L+++H
Sbjct: 606 PRASHPSVTERHLDLRCWVALGARVLTRLAE-HLGEAEVAAELGPLAASLEAAESLDELH 665
Query: 681 FDDAYGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREV-LETPTLRMVPHIGYVSLFP 740
+ G + DFGNHT+ V+L + QG L+R V P L+ V +GYVSLFP
Sbjct: 666 WAPELGVFADFGNHTKAVQLKPRP---PQG-----LVRVVGRPQPQLQYVDALGYVSLFP 725
Query: 741 LMGRIIPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWM 800
L+ R++ P S LG LD++++ W+ +GLRSL+ +SS Y + N+EHD PYWRG +W+
Sbjct: 726 LLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWL 785
Query: 801 NMNYLILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKG 852
N+NYL L AL+HY +GP++ RA + ELR+N++ NV R Y +GF+WEQY+ + G+G
Sbjct: 786 NVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRG 837
BLAST of MS014956 vs. ExPASy Swiss-Prot
Match:
O88941 (Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 SV=1)
HSP 1 Score: 536.6 bits (1381), Expect = 5.2e-151
Identity = 313/796 (39.32%), Postives = 444/796 (55.78%), Query Frame = 0
Query: 85 RVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWL--GVKNG 144
R +T PAP + +WGTYRPH+Y G++ R+P+ LL GLMW G G
Sbjct: 68 RALTLHPAPSALPPDSSSPAVAPEFFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127
Query: 145 R-YFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDWTV 204
+RH C+ D + YGW H+ FG Q + D + L T F+ K SG +GGDW+
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIYDGALRLTTEFV--KRSGGHHGGDWSW 187
Query: 205 RIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGNWQ 264
R+ V + S L S LFFY+ DG + L + SG S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPE--VGAKGQLKFISGHTSELGDFR 247
Query: 265 LHL----KSREDVKIH--YSGFKTAH--FHNLSELVQENL---------GTQARKF---- 324
L L + V H Y+ F +++ L+++V+ +L G ++
Sbjct: 248 LTLLPPTTPGDTVPKHGSYNVFWSSNPGLPLLTDMVKSHLNSWFHHRPPGASPERYLGLP 307
Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRL 384
G L+ + LV Q++ + PF ++ F SG+ G ++ +L G LT L
Sbjct: 308 GSLKWEERGPSGQGQFLVQQVTLKAPFSVEFVFESGSARTG--RDQASEQLVGGQLTRAL 367
Query: 385 KEKQLEFDTKFEKCFGLANK-FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRS 444
+ F +FE+ F L K E +G+ A++ LLGGIGYFYGQ + LP S
Sbjct: 368 ESHAAAFKERFERTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTGMEGS 427
Query: 445 RDSF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 504
+ P L++ VP R FPRGFLWDEGFHQL++ Q WD ++ + LGHWL L+N
Sbjct: 428 EQKMDPSLFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVV-QRWDPHLTREALGHWLGLLN 487
Query: 505 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 564
DGWI REQILG EA ++VP EF+ Q ++ANPP L L + ++ G + E
Sbjct: 488 ADGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVIHMLEG---------RAPED 547
Query: 565 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 624
+FL RAF RL AWF W + +Q G SY W GRD + LNP+TL SGLDDYPRASH
Sbjct: 548 LAFLRRAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASH 607
Query: 625 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAY 684
PS ERH+DLRCW+ L A + ++E + + + LAA +L L+++H+
Sbjct: 608 PSAAERHLDLRCWVTLGARVLSQLAEELGETEAAAELGPLAA-SLEAAGSLDELHWAPEL 667
Query: 685 GTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPT-LRMVPHIGYVSLFPLMGRI 744
G + DFGNHT+ V+L + QG L+R V P L+ V +GYVSLFPL+ ++
Sbjct: 668 GVFADFGNHTKAVQLKSRP---PQG-----LVRVVGRPPARLQYVDALGYVSLFPLLLQL 727
Query: 745 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 804
+ P S LG LD++++ W+ +GLRSLS +S Y + NTEHD PYWRG +W+N+NYL
Sbjct: 728 LEPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYL 787
Query: 805 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 852
L AL+HY +GP++ +A + ELR+N++ NV + Y +GF+WEQY+ + G+G G P
Sbjct: 788 ALGALHHYGRVEGPHKVQAAKLYRELRANVVSNVRQQYQATGFLWEQYSDQDGRGMGCRP 834
BLAST of MS014956 vs. ExPASy TrEMBL
Match:
A0A6J1DD68 (mannosyl-oligosaccharide glucosidase GCS1-like OS=Momordica charantia OX=3673 GN=LOC111019700 PE=3 SV=1)
HSP 1 Score: 1735.7 bits (4494), Expect = 0.0e+00
Identity = 844/854 (98.83%), Postives = 850/854 (99.53%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF
Sbjct: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL
Sbjct: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
G+RARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT
Sbjct: 121 GMRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLR+GHLFFYLADEDGNSLSLSSDA
Sbjct: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRTGHLFFYLADEDGNSLSLSSDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF
Sbjct: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPFKIDM FISGTGFPGSREEERVNRLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMTFISGTGFPGSREEERVNRLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD
Sbjct: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDIYICLDILGHWLDLMNIDGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIYICLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESS+ISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSKISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+EESSYYWHGRDSS IRELNPQTLMSGLDDYPRASHPSED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEESSYYWHGRDSSPIRELNPQTLMSGLDDYPRASHPSED 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRL WKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. ExPASy TrEMBL
Match:
A0A6J1H118 (mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC111458598 PE=3 SV=1)
HSP 1 Score: 1584.7 bits (4102), Expect = 0.0e+00
Identity = 762/854 (89.23%), Postives = 807/854 (94.50%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSRIKSSSAVD D AVRQ+PDIS RHRDR R IRIF+VNLKV+VGF
Sbjct: 1 MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPLIRIFDVNLKVLVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFGAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSK+SEEA E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLNRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+CFGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP SHP SRD
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI++ LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHVSLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQHPSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQHPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL MKGKEVG VYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIMKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPQLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. ExPASy TrEMBL
Match:
A0A6J1KB47 (mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita maxima OX=3661 GN=LOC111492274 PE=3 SV=1)
HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 764/854 (89.46%), Postives = 808/854 (94.61%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M GHSRRNPRSRIKSSSAVD G D AVRQ+PDIS RHRDR R IRIF+VNLKV+VGF
Sbjct: 1 MTGHSRRNPRSRIKSSSAVDGGGDGAVRQAPDISRRHRDRNTLRPWIRIFDVNLKVLVGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
SF+AFFVILYLIRSL+ VGDH+TPRVITPFPAP+VIDLPQFQGEHKESLYWGTYRP LYL
Sbjct: 61 SFVAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTP SLLAGLMW+GV+NGRY MRHVCQ SDELSSYGWTHHNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSSYGWTHHNARDFGHQVLVDHDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLKTKESGSGYGGDW VRIEVNSKKSEEA E+L++GH FFYLADEDGN+LSLS DA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKKSEEAKELLKTGHHFFYLADEDGNALSLSRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
M++HETSLLASGSR DIGNWQLHL+SR+DV++H+SGF+T HFHNLSELVQ+NLG QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISGRIPF+ D+AFISGTGFP SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+ FGL NKFDLESTTVGKAAVANLLGGIGYFYGQSKIALP ASHP SRD
Sbjct: 361 EKELEFDTRFEERFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDASHPGSRD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMNIDGW
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNIDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEA+SKVPEEFIPQ+PSNANPPALFLV+RELVHGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEAVSKVPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP++D
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDD 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL +KGKEVGMVYSLAA TLS+FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELIVKGKEVGMVYSLAAKTLSDFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRLSWKEVMVEQGYTTRQLIREV TP LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WIL KQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATEDGPYRERA IRDELRSNLIRNVV NYH++GF+WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAY ES
Sbjct: 841 SLVLLIMAEAYAES 853
BLAST of MS014956 vs. ExPASy TrEMBL
Match:
A0A5A7SUQ8 (Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001990 PE=3 SV=1)
HSP 1 Score: 1534.2 bits (3971), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 795/854 (93.09%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M G SRRNPRSRIKS+ AVD D AVRQ+ D RHRD KSR SIRIFNVNLKVM+GF
Sbjct: 1 MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
F+AFFVILYLI SL+ GDHQTPRVITPFPA +V DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61 CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTPRSLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWT HNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLK+KESGSGYGGDW VRI+VNSKKSEEA+E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
+++HETSLLASGSRSD+GNWQLHL+SR+++K+H+SGFKT H+HNLSELVQEN+G QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISG IPF+ D+AFISG+G P SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+ FGLANKFD ESTTVGKAAV+NLLGGIGYFYG+SKIALPGASHP S+D
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMN+DGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNVDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEALSKVPEEFIPQ+PSNANPPALFLV+REL+HGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP+ED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTED 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL+ KGK + VYS A LS FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRL WKEVM QG+TTRQLIREV E P LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WILGKQLDLISNRS+FWT+YGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATE GPYRERA IRDELRSN+IRNVVRNYH+SG++WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. ExPASy TrEMBL
Match:
A0A1S3BE51 (mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103488898 PE=3 SV=1)
HSP 1 Score: 1534.2 bits (3971), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 795/854 (93.09%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M G SRRNPRSRIKS+ AVD D AVRQ+ D RHRD KSR SIRIFNVNLKVM+GF
Sbjct: 1 MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60
Query: 61 SFIAFFVILYLIRSLMAVGDHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYL 120
F+AFFVILYLI SL+ GDHQTPRVITPFPA +V DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61 CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120
Query: 121 GIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMT 180
GIRARTPRSLLAGLMW+GV+NGRY MRHVCQ SDELS+YGWT HNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
Query: 181 LATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDA 240
L TSFLK+KESGSGYGGDW VRI+VNSKKSEEA+E+L++GHLFFYLADEDGN+LSL+ DA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240
Query: 241 MDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKF 300
+++HETSLLASGSRSD+GNWQLHL+SR+++K+H+SGFKT H+HNLSELVQEN+G QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300
Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLK 360
GRLQLSDTSDDSSNILVFQISG IPF+ D+AFISG+G P SREEERVN LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360
Query: 361 EKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRD 420
EK+LEFDT+FE+ FGLANKFD ESTTVGKAAV+NLLGGIGYFYG+SKIALPGASHP S+D
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420
Query: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGW 480
SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIW+ WDI+I LDILGHWLDLMN+DGW
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWR-WDIHISLDILGHWLDLMNVDGW 480
Query: 481 IPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFL 540
IPREQILGAEALSKVPEEFIPQ+PSNANPPALFLV+REL+HGLK+NAFT ESSEISSFL
Sbjct: 481 IPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFL 540
Query: 541 ERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSED 600
ERAFVRLEAWFQWFNTTQPG+E SSYYWHGRDSSTIRELNP+TLMSGLDDYPRASHP+ED
Sbjct: 541 ERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTED 600
Query: 601 ERHVDLRCWMLLAADCMHSISELSMKGKEVGMVYSLAANTLSNFELLNQMHFDDAYGTYL 660
ERHVDLRCWMLLAADCMHSISEL+ KGK + VYS A LS FELLNQMHFDDA+GTYL
Sbjct: 601 ERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYL 660
Query: 661 DFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPES 720
DFGNHTEKVRL WKEVM QG+TTRQLIREV E P LRMVPHIGYVSLFPLMGRIIPPES
Sbjct: 661 DFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPES 720
Query: 721 WILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSAL 780
WILGKQLDLISNRS+FWT+YGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSAL
Sbjct: 721 WILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSAL 780
Query: 781 NHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWT 840
NHYATE GPYRERA IRDELRSN+IRNVVRNYH+SG++WEQYNQKTGKGKGAHPFTGWT
Sbjct: 781 NHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWT 840
Query: 841 SLVLLIMAEAYTES 855
SLVLLIMAEAYTES
Sbjct: 841 SLVLLIMAEAYTES 853
BLAST of MS014956 vs. TAIR 10
Match:
AT1G67490.1 (glucosidase 1 )
HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 547/856 (63.90%), Postives = 659/856 (76.99%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M G SRR+ R RIKSSS + G D+ P I R + K SI F NLK++VG
Sbjct: 1 MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60
Query: 61 SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
+ VI ++I L+ G + Q PRVITPFPAP+V+DL FQGEHKESLYWGTYRP
Sbjct: 61 IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120
Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
H+Y G+RARTP SL+AGLMWLGVK+ Y MRH C++SD+LS++GW HN RD+G Q LV+
Sbjct: 121 HVYFGVRARTPLSLVAGLMWLGVKDEMYVMRHFCENSDDLSTFGWREHNGRDYGRQELVE 180
Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
DM + TSF+K+K G GYGGDW VRI+V +K D++ RS HLFFYLADE GN L+L
Sbjct: 181 NDMVIETSFVKSKGDGLGYGGDWAVRIDVKNKGLN--DDVKRSAHLFFYLADEGGNVLNL 240
Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
D +D +SLL SGSR D+G+WQ+HLKS+ ++ HYSGFKT H +NLS+LVQ+NL Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300
Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG S E+R+ LTG L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360
Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
D LK+K EFD KF +CF L+ K D E+ VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420
Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480
Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+ ++ F A E E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540
Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
SFLERA VRL+AWFQWFNT+Q G+E SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600
Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
PSEDERHVDLRCWM LAADCMHSI+EL K ++ Y+ LSNF LLNQMH+D
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660
Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
YG Y DFGNHTEKV+L WKEV+ E G +RQL+R+ P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720
Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 780
Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
ILS+L HY+ DGPYRE++ I ELRSNLIRNVVRNY+++G+IWEQY+Q G GKG
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 840
Query: 841 FTGWTSLVLLIMAEAY 852
FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 849
BLAST of MS014956 vs. TAIR 10
Match:
AT1G24320.1 (Six-hairpin glycosidases superfamily protein )
HSP 1 Score: 1026.5 bits (2653), Expect = 1.2e-299
Identity = 484/773 (62.61%), Postives = 600/773 (77.62%), Query Frame = 0
Query: 80 DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRPHLYLGIRARTPRSLLAGLMWLGV 139
+ Q PRVITPFP+P++ DL FQGEHKESLYWGTYRP +Y G+RARTP+SL+AGLMWL +
Sbjct: 17 ESQRPRVITPFPSPKLTDLSMFQGEHKESLYWGTYRPQMYFGVRARTPKSLVAGLMWLVM 76
Query: 140 KNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVDQDMTLATSFLKTKESGSGYGGDW 199
K+G+ MRH C++S++L S+GW HN RDFG Q L +Q+M L TSF+K++E GYGGDW
Sbjct: 77 KDGKPVMRHFCENSNDLKSFGWKEHNGRDFGRQELFEQNMVLETSFVKSEEGSLGYGGDW 136
Query: 200 TVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSLSSDAMDMHETSLLASGSRSDIGN 259
++RI V + ++ DE+ RS HLFFYLADE N ++L D + + E+S+LASGSR D+GN
Sbjct: 137 SIRINVQNMLND--DEVKRSTHLFFYLADEGCNGVNLGKDVLRLKESSVLASGSRQDVGN 196
Query: 260 WQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQARKFGRLQLSDTSDDSSNILVFQ 319
WQ+HLKS+ ++ HY GFKT NLS LVQ+NL Q K G LQLSD+S+DSSNI VFQ
Sbjct: 197 WQMHLKSQNHLETHYCGFKTPDIVNLSGLVQQNLTAQEEKSGLLQLSDSSEDSSNIYVFQ 256
Query: 320 ISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLTDRLKEKQLEFDTKFEKCFGLANK 379
IS ID+AF+SG S E R+ LTG L+ L+EK + F+ KF +CF L++K
Sbjct: 257 ISTTNQSTIDIAFVSGIKEETSNMENRIMSLTGLPLSSLLEEKHIAFNAKFNECFNLSDK 316
Query: 380 FDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHPRSRDSFLFYWPAELYTAVPCRPV 439
D E+ VGKAA+ N+LGGIGYFYGQSKI P + +S D FL YWPAELYTAVP RP
Sbjct: 317 LDPETLVVGKAAIGNMLGGIGYFYGQSKIHFPKSPLAKSEDDFLLYWPAELYTAVPSRPR 376
Query: 440 FPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEF 499
FPRGFLWDEGFHQLLIW+ WD++I L+I+G+WLDLMNIDGWIPREQ+LGAEALSK+P+++
Sbjct: 377 FPRGFLWDEGFHQLLIWR-WDVHITLEIVGNWLDLMNIDGWIPREQVLGAEALSKIPKQY 436
Query: 500 IPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEISSFLERAFVRLEAWFQWFNTTQP 559
+ Q PSN NPP L LV+R+L++G++ F + +I SFL+RAFVRL+AWF+WFNT+Q
Sbjct: 437 VVQFPSNGNPPTLLLVIRDLINGIRTEKFNEADRDKILSFLDRAFVRLDAWFKWFNTSQI 496
Query: 560 GREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASHPSEDERHVDLRCWMLLAADCMHS 619
G+E+ SYYWHGRD+ T RELNPQ+L SGLDDYPRASHP+EDERHVDLRCWM LAAD M+S
Sbjct: 497 GKEKGSYYWHGRDNITNRELNPQSLSSGLDDYPRASHPNEDERHVDLRCWMYLAADSMNS 556
Query: 620 ISELSMKGK-EVGMVYSLAANTLSNFELLNQMHFDDAYGTYLDFGNHTEKVRLSWKEVMV 679
I E K V YS A LS+F LLNQMH+D +G YLDFGNHTE+VRL WKEV+
Sbjct: 557 IQEFMGKNDILVTEDYSSIAKLLSDFTLLNQMHYDKDHGAYLDFGNHTEEVRLVWKEVIH 616
Query: 680 EQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSVFWT 739
E G+ +R+L+RE P LR+VPHIGYVS FP M RIIP +S IL KQLDLISN ++ W+
Sbjct: 617 EDGHLSRELVRETYGKPELRLVPHIGYVSFFPFMFRIIPADSSILDKQLDLISNGNIVWS 676
Query: 740 NYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALNHYATEDGPYRERALTIR 799
+YGL SL+KTSSLYMKYN+EH APYWRG IWMNMNY+ILS+L+HY+T DGPY +A TI
Sbjct: 677 DYGLLSLAKTSSLYMKYNSEHQAPYWRGAIWMNMNYMILSSLHHYSTVDGPYNSKARTIY 736
Query: 800 DELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAY 852
+ELRSNLIRNVVRNY Q+G IWE Y+Q G G+GA FTGW++L+LLIM+E Y
Sbjct: 737 EELRSNLIRNVVRNYDQTGIIWEHYDQTKGTGEGARVFTGWSALILLIMSEEY 786
BLAST of MS014956 vs. TAIR 10
Match:
AT1G67490.2 (glucosidase 1 )
HSP 1 Score: 986.9 bits (2550), Expect = 1.0e-287
Identity = 496/856 (57.94%), Postives = 594/856 (69.39%), Query Frame = 0
Query: 1 MPGHSRRNPRSRIKSSSAVDSGDDDAVRQSPDISTRHRDRTKSRHSIRIFNVNLKVMVGF 60
M G SRR+ R RIKSSS + G D+ P I R + K SI F NLK++VG
Sbjct: 1 MTGASRRSARGRIKSSS-LSPGSDEGSAYPPSI---RRGKGKELVSIGAFKTNLKILVGL 60
Query: 61 SFIAFFVILYLIRSLMAVG----DHQTPRVITPFPAPRVIDLPQFQGEHKESLYWGTYRP 120
+ VI ++I L+ G + Q PRVITPFPAP+V+DL FQGEHKESLYWGTYRP
Sbjct: 61 IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120
Query: 121 HLYLGIRARTPRSLLAGLMWLGVKNGRYFMRHVCQDSDELSSYGWTHHNARDFGHQVLVD 180
H+Y G+RAR
Sbjct: 121 HVYFGVRARL-------------------------------------------------- 180
Query: 181 QDMTLATSFLKTKESGSGYGGDWTVRIEVNSKKSEEADEMLRSGHLFFYLADEDGNSLSL 240
D++ RS HLFFYLADE GN L+L
Sbjct: 181 ------------------------------------NDDVKRSAHLFFYLADEGGNVLNL 240
Query: 241 SSDAMDMHETSLLASGSRSDIGNWQLHLKSREDVKIHYSGFKTAHFHNLSELVQENLGTQ 300
D +D +SLL SGSR D+G+WQ+HLKS+ ++ HYSGFKT H +NLS+LVQ+NL Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300
Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFKIDMAFISGTGFPGSREEERVNRLTGSSLT 360
ARKFGRLQLSDTS+DSSNI +FQISGR+PF ID+ FISG S E+R+ LTG L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360
Query: 361 DRLKEKQLEFDTKFEKCFGLANKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPGASHP 420
D LK+K EFD KF +CF L+ K D E+ VG+ A+AN+LGGIGYFYGQSKI +P ++ P
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420
Query: 421 RSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWQAWDIYICLDILGHWLDLMN 480
SRD+FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIW+ WDI I LDI+GHWLDL+N
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWR-WDIRITLDIVGHWLDLLN 480
Query: 481 IDGWIPREQILGAEALSKVPEEFIPQHPSNANPPALFLVMRELVHGLKRNAFTAKESSEI 540
IDGWIPREQILGAEALSKVPEEF+ Q+PSN NPP LFLV+R+L+ ++ F A E E+
Sbjct: 481 IDGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEV 540
Query: 541 SSFLERAFVRLEAWFQWFNTTQPGREESSYYWHGRDSSTIRELNPQTLMSGLDDYPRASH 600
SFLERA VRL+AWFQWFNT+Q G+E SY+WHGRD++T +ELNP+TL SGLDDYPRASH
Sbjct: 541 LSFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASH 600
Query: 601 PSEDERHVDLRCWMLLAADCMHSISELSMKGKEVGMV-YSLAANTLSNFELLNQMHFDDA 660
PSEDERHVDLRCWM LAADCMHSI+EL K ++ Y+ LSNF LLNQMH+D
Sbjct: 601 PSEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSD 660
Query: 661 YGTYLDFGNHTEKVRLSWKEVMVEQGYTTRQLIREVLETPTLRMVPHIGYVSLFPLMGRI 720
YG Y DFGNHTEKV+L WKEV+ E G +RQL+R+ P L++VPH+GYVS FP M RI
Sbjct: 661 YGAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRI 720
Query: 721 IPPESWILGKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYL 780
IPP+S IL KQLDLISNRS+ W++YGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+
Sbjct: 721 IPPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYM 765
Query: 781 ILSALNHYATEDGPYRERALTIRDELRSNLIRNVVRNYHQSGFIWEQYNQKTGKGKGAHP 840
ILS+L HY+ DGPYRE++ I ELRSNLIRNVVRNY+++G+IWEQY+Q G GKG
Sbjct: 781 ILSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRL 765
Query: 841 FTGWTSLVLLIMAEAY 852
FTGW++L LLIM+E Y
Sbjct: 841 FTGWSALTLLIMSEDY 765
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022151803.1 | 0.0e+00 | 98.83 | mannosyl-oligosaccharide glucosidase GCS1-like [Momordica charantia] | [more] |
XP_022957119.1 | 0.0e+00 | 89.23 | mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita moschata] | [more] |
XP_022997334.1 | 0.0e+00 | 89.46 | mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita maxima] | [more] |
XP_023541215.1 | 0.0e+00 | 88.99 | mannosyl-oligosaccharide glucosidase GCS1 [Cucurbita pepo subsp. pepo] | [more] |
KAG6601592.1 | 0.0e+00 | 88.64 | Mannosyl-oligosaccharide glucosidase GCS1, partial [Cucurbita argyrosperma subsp... | [more] |
Match Name | E-value | Identity | Description | |
F4HTM3 | 0.0e+00 | 63.90 | Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS... | [more] |
Q84M89 | 1.7e-298 | 62.61 | Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1 | [more] |
Q80UM7 | 6.6e-154 | 39.82 | Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1 | [more] |
Q13724 | 1.2e-152 | 39.58 | Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5 | [more] |
O88941 | 5.2e-151 | 39.32 | Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DD68 | 0.0e+00 | 98.83 | mannosyl-oligosaccharide glucosidase GCS1-like OS=Momordica charantia OX=3673 GN... | [more] |
A0A6J1H118 | 0.0e+00 | 89.23 | mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
A0A6J1KB47 | 0.0e+00 | 89.46 | mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita maxima OX=3661 GN=LOC1114... | [more] |
A0A5A7SUQ8 | 0.0e+00 | 86.07 | Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=11... | [more] |
A0A1S3BE51 | 0.0e+00 | 86.07 | mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103... | [more] |