MS014943 (gene) Bitter gourd (TR) v1

Overview
NameMS014943
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionalpha-ketoglutarate-dependent dioxygenase alkB
Locationscaffold2: 136655 .. 138521 (+)
RNA-Seq ExpressionMS014943
SyntenyMS014943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGGATCCGACAAAGGCACCGACGATTTGGAGCGCACCGCTTTCAGAATAGCAGAAAAGAAATACAAGTTCTACTACGACGACACCTTTAAATCTTCCAAAAAGTATATTTCTTCACAATTTCGCTTCAGGTTTTGTTTCATCAATTGGCACCGGACTCGTTGTAAGAGGTGATGTTTCAAATCCGGTTTATGGTTTCTCTCAGGAAAAAACTACCGAAACAAGTGGATTTGTCGGAGGTTATCGATTTCAAGCGCATTCTAGAATGTTACAATCACGATGGCGCACTTCCGCTGGGCATGAACGCGACTCAGTGCGATCTCGATGGGCCAGTTTTCTGCTTGGAGAATCGTCCTGGTATTGTCAATTCCAGTTTTATGCCGTTTCAAAGAACCCCAAAAAAAATATCCCAAATTCAGTTGTTCTTTATGTGTCTGTAATGTAGGATTTTATTTCATTCCTGGAGCGTTAAGCTTAGAAGAGCAATGCCAATGGATTAGGGAGAGTCTAACGAGTTTCCCGCAGCCTCCTAACAGAACCAATCACAACGCTATTTATGGACCAATTCAAGACCTGTTCATTGCAGCAAAGGAAAAGAGAGTTTTAGTGGAAGCAAATGAAATCTCTGCTTTCAACGTTGATTCCGACATTGAACCTTCGGTTAGCACTGGAAATACTCATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGAAGGACGACCTCCAAATCAGTTCCAGCTTCTGTGTTACTTAGAAAGTTGCGTTGGAGCACCCTCGGCCTACAATTTGATTGGTCCAAGGTGAGCCTAACAAGAAAACTTCATATTTTGCTGGTATTTTAATAATTTGAACACTGCGTTTTCAACATTTTAAATGGTTTCGATATGGAAGTTGAAACTTTAGAAGGTCATTCATTGTGGGGATTTTTTTAACGAAATGTCAAAACCTTCAGCATTGTCTCTGTCGATATGTTTGATGTGTTATCTTTTGAATTAATGTCCTTGTAATGGCGTAGCGAAGCTATGACATATCTCTGCTACATAATAAGATGCCCTCTGCACTGTGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCGGCTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTGAATTATTTTGCTTCGGGTAACCAAACACAACTTTGTTCATTAACATCAATAAAATGCTAAATATTGCATTGAAAGTTAACAGCATCTTGTCATGACTCTGACACGTTATCGATATTCCAGGCGACAGTCTCGGGGGTCACCTAGATGACATGGAAGCAGACTGGAGCAAGCCAATTGTTAGCATGAGGTATTATTCTAACTTATCCTCTTTGAATTGCATTTTTTTCCTGATATGTTAAGAGGACATGAGAGATACTTATTATATTCATATCTGCCTGCCTCCACGATTTTCTTCACCCAGTTTGGGATGCAAAGCTATTTTCCTCTTGGGAGGCAAGTCGAGACAGGATCCACCGGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTTATGGCTGGAGAAGCGAGGGAATGTTTTCATGGTAACGTGTTCTTCCATATCTACTCTTACATTGTGTTGAAATGTTATCTCTGTAGGCGATACATGCACTTGGATATCCGATTCCAACAATGCTGAGTAATTCATTCATTTGTAGAAAGATCATTAAATTCCCAAAACATGCTCTGTGGATGTCTTGTAGGTGTACCTCGGATCTTCATCGATGTAGAAACTGACGAAACTTCTCTTCTTGAAAAGCAGTTTTCAAATCAAGATGATTTGCATTTTCTGGAATACATTAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTC

mRNA sequence

ATGTACGGATCCGACAAAGGCACCGACGATTTGGAGCGCACCGCTTTCAGAATAGCAGAAAAGAAATACAAGTTCTACTACGACGACACCTTTAAATCTTCCAAAAAGAAAAAACTACCGAAACAAGTGGATTTGTCGGAGGTTATCGATTTCAAGCGCATTCTAGAATGTTACAATCACGATGGCGCACTTCCGCTGGGCATGAACGCGACTCAGTGCGATCTCGATGGGCCAGTTTTCTGCTTGGAGAATCGTCCTGGATTTTATTTCATTCCTGGAGCGTTAAGCTTAGAAGAGCAATGCCAATGGATTAGGGAGAGTCTAACGAGTTTCCCGCAGCCTCCTAACAGAACCAATCACAACGCTATTTATGGACCAATTCAAGACCTGTTCATTGCAGCAAAGGAAAAGAGAGTTTTAGTGGAAGCAAATGAAATCTCTGCTTTCAACGTTGATTCCGACATTGAACCTTCGGTTAGCACTGGAAATACTCATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGAAGGACGACCTCCAAATCAGTTCCAGCTTCTGTGTTACTTAGAAAGTTGCGTTGGAGCACCCTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCTACATAATAAGATGCCCTCTGCACTGTGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCGGCTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTGAATTATTTTGCTTCGGGCGACAGTCTCGGGGGTCACCTAGATGACATGGAAGCAGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGATGCAAAGCTATTTTCCTCTTGGGAGGCAAGTCGAGACAGGATCCACCGGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTTATGGCTGGAGAAGCGAGGGAATGTTTTCATGGTGTACCTCGGATCTTCATCGATGTAGAAACTGACGAAACTTCTCTTCTTGAAAAGCAGTTTTCAAATCAAGATGATTTGCATTTTCTGGAATACATTAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTC

Coding sequence (CDS)

ATGTACGGATCCGACAAAGGCACCGACGATTTGGAGCGCACCGCTTTCAGAATAGCAGAAAAGAAATACAAGTTCTACTACGACGACACCTTTAAATCTTCCAAAAAGAAAAAACTACCGAAACAAGTGGATTTGTCGGAGGTTATCGATTTCAAGCGCATTCTAGAATGTTACAATCACGATGGCGCACTTCCGCTGGGCATGAACGCGACTCAGTGCGATCTCGATGGGCCAGTTTTCTGCTTGGAGAATCGTCCTGGATTTTATTTCATTCCTGGAGCGTTAAGCTTAGAAGAGCAATGCCAATGGATTAGGGAGAGTCTAACGAGTTTCCCGCAGCCTCCTAACAGAACCAATCACAACGCTATTTATGGACCAATTCAAGACCTGTTCATTGCAGCAAAGGAAAAGAGAGTTTTAGTGGAAGCAAATGAAATCTCTGCTTTCAACGTTGATTCCGACATTGAACCTTCGGTTAGCACTGGAAATACTCATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGAAGGACGACCTCCAAATCAGTTCCAGCTTCTGTGTTACTTAGAAAGTTGCGTTGGAGCACCCTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCTACATAATAAGATGCCCTCTGCACTGTGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCGGCTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTGAATTATTTTGCTTCGGGCGACAGTCTCGGGGGTCACCTAGATGACATGGAAGCAGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGATGCAAAGCTATTTTCCTCTTGGGAGGCAAGTCGAGACAGGATCCACCGGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTTATGGCTGGAGAAGCGAGGGAATGTTTTCATGGTGTACCTCGGATCTTCATCGATGTAGAAACTGACGAAACTTCTCTTCTTGAAAAGCAGTTTTCAAATCAAGATGATTTGCATTTTCTGGAATACATTAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTC

Protein sequence

MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNHDGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRTTSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQVF
Homology
BLAST of MS014943 vs. NCBI nr
Match: XP_022151981.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Momordica charantia])

HSP 1 Score: 738.4 bits (1905), Expect = 2.9e-209
Identity = 361/362 (99.72%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE
Sbjct: 181 TCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. NCBI nr
Match: XP_022945036.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata])

HSP 1 Score: 674.5 bits (1739), Expect = 5.2e-190
Identity = 328/362 (90.61%), Postives = 342/362 (94.48%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRILECYN 
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPS+  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN++PSALCQL KRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK+FS++DDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. NCBI nr
Match: KAG6573589.1 (Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.0 bits (1730), Expect = 5.7e-189
Identity = 327/362 (90.33%), Postives = 340/362 (93.92%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRI ECYN 
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPSV  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN++PSALCQL KRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK FS++DDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. NCBI nr
Match: XP_022966806.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima])

HSP 1 Score: 665.6 bits (1716), Expect = 2.4e-187
Identity = 327/362 (90.33%), Postives = 340/362 (93.92%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRILECYN 
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQ IRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPSV  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN +PSALCQLAKRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK+FS++DDLHFLEY+RTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. NCBI nr
Match: XP_023541203.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.8 bits (1714), Expect = 4.1e-187
Identity = 324/362 (89.50%), Postives = 340/362 (93.92%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRILECYN 
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFI GALSLEEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFISGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEK+VLVE NEISA NVDSD EPS+  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN++PSALCQL KRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVS+SLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSLSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK+FS++DDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 478.0 bits (1229), Expect = 9.3e-134
Identity = 239/363 (65.84%), Postives = 279/363 (76.86%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MY S   +DD +RTAFR AEKKYK YY+   K S+KKKLPK +DLSE++DF  I + +N+
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DG LP G+  ++ D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLTSFPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+           D+         + WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQ----------DDL-------TNNKWKFYEEVDIEKATR 180

Query: 181 TTSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240
           ++ KSV ASVLLRKLRWSTLGLQFDWSKR+YD+SL HN +P ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIF   E  +   LE + S++    F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 345

BLAST of MS014943 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-42
Identity = 114/352 (32.39%), Postives = 181/352 (51.42%), Query Frame = 0

Query: 32  KSSKKKKLPKQ----VDLSEVIDF-----------KRILECYN----HDGALPLGMNATQ 91
           KS+  K +PK+    +D S V+DF           K I++C +    HD           
Sbjct: 50  KSANGKNIPKEKREPIDYSPVLDFHNLENNTEENKKLIIDCTSNVTTHDFEFNRDTEFYL 109

Query: 92  CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNHNAIYGPIQDLFI 151
              +  V+ L+  PGFYFI    +  +Q +WI+ +L  +  PPN  N    +GPI++L+ 
Sbjct: 110 HPREWKVYGLQGYPGFYFIKSPFTASQQKKWIKHALEDYADPPNNNNITLFHGPIKNLW- 169

Query: 152 AAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRTTSKSVPASVLLR 211
              EK ++ E  +    + D +IE      + +G     E   + R+          LL 
Sbjct: 170 KNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG-----EPLPTYRQ----------LLD 229

Query: 212 KLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAAIVNYFA 271
           KL WSTLG Q+ W+ R Y     + + P  L +L +++A A     + +  EAA VN+++
Sbjct: 230 KLAWSTLGYQYQWTPRLYS-EEFYEEFPDDLQELVQKIAIAT--KFDPYVAEAATVNFYS 289

Query: 272 SGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEAR 331
               +GGHLDD E +  KPI+S+S G  A+FL+G ++R   PV +F+RSGD+V+M G +R
Sbjct: 290 EDSIMGGHLDDAEQEMEKPIISISFGSTAVFLMGAETRDIAPVPLFIRSGDIVIMGGRSR 349

Query: 332 ECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYI--RTSRININIRQVF 363
            C+HGV +I   VE      L  +  +QD  + ++++  +  R+NIN RQVF
Sbjct: 350 YCYHGVAKI---VENSFDLGLIDENDDQDLKYKIQWLKEKNRRVNINTRQVF 379

BLAST of MS014943 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-30
Identity = 92/294 (31.29%), Postives = 142/294 (48.30%), Query Frame = 0

Query: 82  LENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLV 141
           L+  PGF FIP       Q  W+++ L  + Q PN  N         D  ++ +E + L 
Sbjct: 93  LKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCN--------LDKHMSKEETQDLW 152

Query: 142 EANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRTTSKSVPASVLLRKLRWSTLGL 201
           E ++                      +F+     + RR  S       LL KLRW T+G 
Sbjct: 153 EQSK----------------------EFLRYKEATKRRPRS-------LLEKLRWVTVGY 212

Query: 202 QFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAAIVNYFASGDSLGGHL 261
            ++W  + Y     +   PS L  L++++AAA     E+F+ EA I+NY+    +LG H+
Sbjct: 213 HYNWDSKKYSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLGIHV 272

Query: 262 DDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 321
           D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VPR+
Sbjct: 273 DRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRV 332

Query: 322 FIDVETD------ETSL--------LEKQFSNQDDLHFLEYIRTSRININIRQV 362
             + E +      E  L        + +  S +D      Y++T+R+N+ +RQV
Sbjct: 333 LPNPEGEGLPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQV 346

BLAST of MS014943 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 94/294 (31.97%), Postives = 139/294 (47.28%), Query Frame = 0

Query: 82  LENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLV 141
           LE  PGF FIP       Q  W+++ L  + Q PN  N         D  +  +E + L 
Sbjct: 93  LEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVCN--------LDKHMTKEETQGLW 152

Query: 142 EANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRTTSKSVPASVLLRKLRWSTLGL 201
           E ++                      + +    V+ RR  S       LL +LRW TLG 
Sbjct: 153 EQSK----------------------EVLRSKEVTKRRPRS-------LLERLRWVTLGY 212

Query: 202 QFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAAIVNYFASGDSLGGHL 261
            ++W  + Y     +   PS L  L++++A A    G  F+ EA I+NY+    +LG H+
Sbjct: 213 HYNWDSKKYSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLGIHV 272

Query: 262 DDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 321
           D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VPR+
Sbjct: 273 DRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVPRV 332

Query: 322 FIDVETD------ETSL--------LEKQFSNQDDLHFLEYIRTSRININIRQV 362
               + +      ET L        L +  S +D      Y+RT+R+N+ +RQV
Sbjct: 333 LPHPDGECLPHCLETPLPAVLPSNSLVEPCSVEDWQVCATYLRTARVNMTVRQV 346

BLAST of MS014943 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-27
Identity = 94/355 (26.48%), Postives = 152/355 (42.82%), Query Frame = 0

Query: 12  ERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNHDGALPLGMNAT 71
           +   FR+ EK+YK   D          +P   D+SEV+D          +     G  A 
Sbjct: 8   QANVFRLEEKRYKCRAD---------TIP---DMSEVLD---------PNDPQSFGFEAL 67

Query: 72  QCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESL-TSFPQPPNRTNHNAIYGPIQDL 131
             ++   VF  +  PG   +   +S E Q Q ++  + T    P N+TN +  Y      
Sbjct: 68  -VEIKPRVFSFQKAPGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFY------ 127

Query: 132 FIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFV----EENTVSSRRTTSKSVP 191
                                       +  GN   W+       E+ +     T     
Sbjct: 128 ---------------------------QLPLGNDSIWRRYYNGDGESIIDGLGETKPLTV 187

Query: 192 ASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAA 251
             ++ +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K EAA
Sbjct: 188 DRLVHKKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKAEAA 247

Query: 252 IVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVL 311
           IVN+++ GD+L  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+
Sbjct: 248 IVNFYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVI 298

Query: 312 MAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQV 362
           M G +R+ FH VP+I  +   +      K +          +I   R+N N+RQV
Sbjct: 308 MTGTSRKAFHAVPKIIPNSTPNYLLTGNKAWDG--------WISRKRVNFNVRQV 298

BLAST of MS014943 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.4e-209
Identity = 361/362 (99.72%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE
Sbjct: 181 TCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.5e-190
Identity = 328/362 (90.61%), Postives = 342/362 (94.48%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRILECYN 
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPS+  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN++PSALCQL KRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK+FS++DDLHFLEYIRTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.2e-187
Identity = 327/362 (90.33%), Postives = 340/362 (93.92%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRILECYN 
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQ IRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPSV  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGEE 240
           T KSVPAS LLRKLRWSTLGLQFDWSKRSYDISL HN +PSALCQLAKRMAAAAMP GEE
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGEE 240

Query: 241 FKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLR 300
           FKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLR
Sbjct: 241 FKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLR 300

Query: 301 SGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQ 360
           SGDVVLMAGEARECFHGVPRIF D E++E SLLEK+FS++DDLHFLEY+RTSRININIRQ
Sbjct: 301 SGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQ 360

Query: 361 VF 363
           VF
Sbjct: 361 VF 362

BLAST of MS014943 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.3e-183
Identity = 316/363 (87.05%), Postives = 335/363 (92.29%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPK VDLSEVIDFK ILE Y  
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DG+LP+G+NAT CDLDGPVFCLENRPGFYFIPGALSL+EQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180
           NAIYGPIQDLFIAAKE +VLVE +EIS F +DSD+EPS+S GNTH WKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 TS-KSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240
           T+ KS+PASVLLRKLRWSTLGLQFDWSKRSY+ISL HNK+PSALCQLAKRMAAAAMP GE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFID E++E S LE   +NQDDLH LEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIR 360

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 363

BLAST of MS014943 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 315/363 (86.78%), Postives = 334/363 (92.01%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPK VDLSEVIDFK ILE Y  
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DG+LP+G+ AT CDLD PVFCLENRPGFYFIPGALSL+EQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRR- 180
           NAIYGPIQDLFIAAKEK+VLVE +EIS FN+DSD+EPS+S G+TH WKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 TTSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240
           T  KS+ ASVLLRKLRWSTLGLQFDWSKRSY+ISL HNK+PSALCQLAKRMAAAAMP GE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFID E++E S LE+  SNQDDLHFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIR 360

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 363

BLAST of MS014943 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 478.0 bits (1229), Expect = 6.6e-135
Identity = 239/363 (65.84%), Postives = 279/363 (76.86%), Query Frame = 0

Query: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60
           MY S   +DD +RTAFR AEKKYK YY+   K S+KKKLPK +DLSE++DF  I + +N+
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120
           DG LP G+  ++ D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLTSFPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+           D+         + WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQ----------DDL-------TNNKWKFYEEVDIEKATR 180

Query: 181 TTSKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240
           ++ KSV ASVLLRKLRWSTLGLQFDWSKR+YD+SL HN +P ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIF   E  +   LE + S++    F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 363
           QVF
Sbjct: 361 QVF 345

BLAST of MS014943 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 4.6e-11
Identity = 43/122 (35.25%), Postives = 63/122 (51.64%), Query Frame = 0

Query: 243 PEAAIVNYFASGDSLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++S   LG H D  E++ S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 303 LRSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININI 361
           L SGDV+L  G +R+ FHGV  I  D  T   +LL++             +R  R+N+  
Sbjct: 406 LESGDVLLFGGRSRKVFHGVRSIRKD--TAPKALLQE-----------TSLRPGRLNLTF 454

BLAST of MS014943 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 5.1e-10
Identity = 42/121 (34.71%), Postives = 59/121 (48.76%), Query Frame = 0

Query: 243 PEAAIVNYFASGDSLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 303 LRSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININI 360
           L SGDV++  GE+R  FHGV  I     +   SLL +             +RT R+N+  
Sbjct: 338 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLLNE-----------SKLRTGRLNLTF 385

BLAST of MS014943 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 5.1e-10
Identity = 42/121 (34.71%), Postives = 59/121 (48.76%), Query Frame = 0

Query: 243 PEAAIVNYFASGDSLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 302
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 303 LRSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININI 360
           L SGDV++  GE+R  FHGV  I     +   SLL +             +RT R+N+  
Sbjct: 393 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLLNE-----------SKLRTGRLNLTF 440

BLAST of MS014943 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 32/104 (30.77%), Postives = 43/104 (41.35%), Query Frame = 0

Query: 243 PEAAIVNYFASGDSLGGH----LDDMEADWSK---------------------PIVSMSL 302
           P+  +VN++ S   LG H     D    D+ K                     PIVS S+
Sbjct: 351 PDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSI 410

Query: 303 GCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 322
           G  A FL G +   D    + L SGDV++    +R  FHGV  I
Sbjct: 411 GDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSI 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151981.12.9e-20999.72alpha-ketoglutarate-dependent dioxygenase alkB [Momordica charantia][more]
XP_022945036.15.2e-19090.61alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata][more]
KAG6573589.15.7e-18990.33Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma ... [more]
XP_022966806.12.4e-18790.33alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima][more]
XP_023541203.14.1e-18789.50alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SA989.3e-13465.84Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G... [more]
Q54N081.7e-4232.39Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44... [more]
Q136861.5e-3031.29Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2[more]
P0CB421.3e-2931.97Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1[more]
O600661.2e-2726.48Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str... [more]
Match NameE-valueIdentityDescription
A0A6J1DG901.4e-20999.72alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN... [more]
A0A6J1FZQ92.5e-19090.61alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HQB41.2e-18790.33alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A0A0KS002.3e-18387.05Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3BE321.5e-18286.78LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m... [more]
Match NameE-valueIdentityDescription
AT1G11780.16.6e-13565.84oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
AT3G14160.14.6e-1135.252-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.15.1e-1034.712-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.25.1e-1034.712-oxoglutarate-dependent dioxygenase family protein [more]
AT3G14140.11.3e-0530.772-oxoglutarate-dependent dioxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 163..361
e-value: 3.9E-47
score: 162.5
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 183..359
e-value: 5.3E-40
score: 137.7
NoneNo IPR availablePANTHERPTHR16557:SF8ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKBcoord: 1..362
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 86..361
IPR004574Alkylated DNA repair protein AlkBPANTHERPTHR16557ALKYLATED DNA REPAIR PROTEIN ALKB-RELATEDcoord: 1..362
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 242..362
score: 9.229217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014943.1MS014943.1mRNA