MS014904 (gene) Bitter gourd (TR) v1

Overview
NameMS014904
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING/U-box superfamily protein
Locationscaffold977: 507773 .. 509220 (-)
RNA-Seq ExpressionMS014904
SyntenyMS014904
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTAGACTGTCCGAGAGAGAATGATGGAGGTTGTGTTCAAATGAGATTATCTTACAGTCCAGCAGCTCCATTTTTCCTTTTTCTTGTTCAATGGACTGACTGTTCCCTTGCTGGTGCCTTGGGACTGCTTAGAATCCTTATCTATGTGGTATATGCCCTTTTCATTTTGGTACTACTATTTGGCAATATTCATCAGTTGGTAATTGTTCTTGGTTTTGTTTGTTTATGTTGTCTTGATATTTTTTCCATTGGAGGCCCTTAACTGTAATAATCAAGTTATATTCTGATTGATAAACAAATAAATTTTTTTTTAAAAGAAGTCTGATGTTCTATCTCCTGACAATGAAGGTAATTATTGTTTAATTACTTCTTGTCGTTTGCAACTATGTTTTGATGCAGACTTATCCAGATGGAAAGACCACCATGTCCATATACGAGCGAAAAGCTAGCATTAGAGAATTTTATGGTGAGTGAGATTTTCAGAATGCAGCCATAATGTATATTTACTTTACATGAATCTAAACTCTGGTGCAATGAATCTCTTTTGGAACTTGGCTTTCTGAACTTCTTCTTCTTGTTCTTTTTTGGTGGGGTGGCTTCTGGACACGCCTTCTTATCGACAGTGGTAATCTTCCCTTCTCTTCTGCAACTTCAAAGAGGCATCACAGAATTGGAAGATAGAAAACAGAGAGAGGTCTGCAATGCCAGGTACAAAAAAAGGGATGAACTTGGAAGGGGAAAACTCTCAGAAATCGACATTGAAAGAGAAAAGGAGTGTGGAATTTGTATGGAGGTTAACGGCATGGTTGTTCTGCCCAACTGCAACCATTCTTTGTGCTTGAAGTGTTATCGAGACTGGTAAGCTTTTGTGATTTTACTTTTTTGATTGATTACAACGTATTCCCTTCTCCACCCCCCTCCAGAAAGTAGATTAATAATTCAATACTAAATGAAATAAAGAATTATATTGTTCAAACGCGCCCAAAGTTATCCACTTCTAGGAGTAGGACCAGCATTATCTTATAAAGTACTATGAAAAATTATGAAATTAGCATTTCTGTCGGCAAGTCTATAGACAAAATGCATTCTCTCTCATGGGGTAAGATTAAATTCTGTGTTGGATCTGAAAATCTTGTCAGCCATCCATATGCCTTTACATTGCATTTACTGCTAGCATTCTCAGACTCATATTCAACATCATAACTAGAACTACCTTGTCTGTCTATTCCACCCTGCAGGCGCGGTCGTTCTCAGTCGTGTCCCTTCTGTCGGGATAGCCTGAGGAGAGTCAACTCGGGCGATCTCTGGATCTACACAGACAAGGCTGAGATGGTGGATCTAACTTTAATTTTTCAAGAGAATCGCAAGAGACTGTTCATTTACATTGATAAGTTGCCATTGGTTGTTCCAGATTCAGTGTTCCTTCCGCTTGATTCTCATATCAGG

mRNA sequence

GCTTTAGACTGTCCGAGAGAGAATGATGGAGGTTGTGTTCAAATGAGATTATCTTACAGTCCAGCAGCTCCATTTTTCCTTTTTCTTGTTCAATGGACTGACTGTTCCCTTGCTGGTGCCTTGGGACTGCTTAGAATCCTTATCTATGTGACTTATCCAGATGGAAAGACCACCATGTCCATATACGAGCGAAAAGCTAGCATTAGAGAATTTTATGTGGTAATCTTCCCTTCTCTTCTGCAACTTCAAAGAGGCATCACAGAATTGGAAGATAGAAAACAGAGAGAGGTCTGCAATGCCAGGTACAAAAAAAGGGATGAACTTGGAAGGGGAAAACTCTCAGAAATCGACATTGAAAGAGAAAAGGAGTGTGGAATTTGTATGGAGGTTAACGGCATGGTTGTTCTGCCCAACTGCAACCATTCTTTGTGCTTGAAGTGTTATCGAGACTGGCGCGGTCGTTCTCAGTCGTGTCCCTTCTGTCGGGATAGCCTGAGGAGAGTCAACTCGGGCGATCTCTGGATCTACACAGACAAGGCTGAGATGGTGGATCTAACTTTAATTTTTCAAGAGAATCGCAAGAGACTGTTCATTTACATTGATAAGTTGCCATTGGTTGTTCCAGATTCAGTGTTCCTTCCGCTTGATTCTCATATCAGG

Coding sequence (CDS)

GCTTTAGACTGTCCGAGAGAGAATGATGGAGGTTGTGTTCAAATGAGATTATCTTACAGTCCAGCAGCTCCATTTTTCCTTTTTCTTGTTCAATGGACTGACTGTTCCCTTGCTGGTGCCTTGGGACTGCTTAGAATCCTTATCTATGTGACTTATCCAGATGGAAAGACCACCATGTCCATATACGAGCGAAAAGCTAGCATTAGAGAATTTTATGTGGTAATCTTCCCTTCTCTTCTGCAACTTCAAAGAGGCATCACAGAATTGGAAGATAGAAAACAGAGAGAGGTCTGCAATGCCAGGTACAAAAAAAGGGATGAACTTGGAAGGGGAAAACTCTCAGAAATCGACATTGAAAGAGAAAAGGAGTGTGGAATTTGTATGGAGGTTAACGGCATGGTTGTTCTGCCCAACTGCAACCATTCTTTGTGCTTGAAGTGTTATCGAGACTGGCGCGGTCGTTCTCAGTCGTGTCCCTTCTGTCGGGATAGCCTGAGGAGAGTCAACTCGGGCGATCTCTGGATCTACACAGACAAGGCTGAGATGGTGGATCTAACTTTAATTTTTCAAGAGAATCGCAAGAGACTGTTCATTTACATTGATAAGTTGCCATTGGTTGTTCCAGATTCAGTGTTCCTTCCGCTTGATTCTCATATCAGG

Protein sequence

ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR
Homology
BLAST of MS014904 vs. NCBI nr
Match: XP_022150973.1 (uncharacterized protein LOC111018996 isoform X1 [Momordica charantia])

HSP 1 Score: 458.8 bits (1179), Expect = 2.7e-125
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS
Sbjct: 24  ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA
Sbjct: 144 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 204 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 243

BLAST of MS014904 vs. NCBI nr
Match: XP_022150974.1 (uncharacterized protein LOC111018996 isoform X2 [Momordica charantia])

HSP 1 Score: 458.8 bits (1179), Expect = 2.7e-125
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS
Sbjct: 22  ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 81

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER
Sbjct: 82  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 141

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA
Sbjct: 142 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 201

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 202 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 241

BLAST of MS014904 vs. NCBI nr
Match: XP_022150975.1 (E3 ubiquitin-protein ligase rnf8 isoform X3 [Momordica charantia])

HSP 1 Score: 426.0 bits (1094), Expect = 2.0e-115
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 15  MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV 74
           MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV
Sbjct: 1   MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV 60

Query: 75  IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV 134
           IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV
Sbjct: 61  IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV 120

Query: 135 VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK 194
           VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK
Sbjct: 121 VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK 180

Query: 195 RLFIYIDKLPLVVPDSVFLPLDSHIR 221
           RLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 181 RLFIYIDKLPLVVPDSVFLPLDSHIR 206

BLAST of MS014904 vs. NCBI nr
Match: XP_038893737.1 (E3 ubiquitin-protein ligase AIRP2 [Benincasa hispida])

HSP 1 Score: 424.9 bits (1091), Expect = 4.3e-115
Identity = 199/220 (90.45%), Postives = 210/220 (95.45%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRE+DG CVQMRLSYSPAAPFFLFLVQWTDC LAGALGLLRILIYVTYPDGKTTMS
Sbjct: 24  ALDCPRESDGACVQMRLSYSPAAPFFLFLVQWTDCYLAGALGLLRILIYVTYPDGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQ+GITELEDRKQ+EVCNARY +RDE GRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQKGITELEDRKQKEVCNARYNRRDEFGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICME NGMVVLPNCNHSLCLKCY+DWRGRSQSCPFCRDSL+RVNS DLWI+TDK+
Sbjct: 144 EKECGICMEFNGMVVLPNCNHSLCLKCYQDWRGRSQSCPFCRDSLKRVNSSDLWIFTDKS 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EM+DLT I Q+NRKRLF YIDKLPLVVPD VFLP+DSHIR
Sbjct: 204 EMIDLTSILQDNRKRLFAYIDKLPLVVPDPVFLPIDSHIR 243

BLAST of MS014904 vs. NCBI nr
Match: XP_011658560.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Cucumis sativus])

HSP 1 Score: 424.9 bits (1091), Expect = 4.3e-115
Identity = 199/220 (90.45%), Postives = 209/220 (95.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRE+DG CVQMRLSYSPAAPFFLFLVQWTDC LAGALGLLRILIYVTYPDGKTTMS
Sbjct: 24  ALDCPRESDGACVQMRLSYSPAAPFFLFLVQWTDCYLAGALGLLRILIYVTYPDGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQ+GITELEDRKQ+EVCNARY +RDE GRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQKGITELEDRKQKEVCNARYSRRDEFGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICME NGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSL+RVNSGDLWI+TDK+
Sbjct: 144 EKECGICMEFNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLKRVNSGDLWIFTDKS 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EM+DL  I  +NRKRLF YIDKLPLVVPD VFLP+DSHIR
Sbjct: 204 EMIDLNSILHDNRKRLFAYIDKLPLVVPDPVFLPMDSHIR 243

BLAST of MS014904 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-86
Identity = 148/214 (69.16%), Postives = 175/214 (81.78%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           A + P E DGG VQMRLSYSPAA  FLFL+QWTDC  AGALGLLRILIY  Y DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           ++ERK SIREFY V+FPSLLQL  GIT++E+RKQ+E+C+ RY+K+D   +GK+SEID+ER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGIC+E+   VVLP CNHS+C+ CYR+WR RSQSCPFCR SL+RVNSGDLWIYT  A
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLP 215
           E+ DL  I++EN KRL IYIDKLPLV  D   +P
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVP 236

BLAST of MS014904 vs. ExPASy Swiss-Prot
Match: Q9UHC7 (E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 34/92 (36.96%), Postives = 45/92 (48.91%), Query Frame = 0

Query: 118 IEREKE--CGICMEV---------NGMVVLPNCNHSLCLKCYRDWRGRSQ-------SCP 177
           ++R K+  CGICMEV             +L NCNH+ CLKC R WR   Q       SCP
Sbjct: 273 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 332

Query: 178 FCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQE 192
            CR +   V   + W+  +K E   L L ++E
Sbjct: 333 ECRITSNFVIPSEYWV-EEKEEKQKLILKYKE 363

BLAST of MS014904 vs. ExPASy Swiss-Prot
Match: Q13434 (Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P PE=5 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.3e-07
Identity = 31/88 (35.23%), Postives = 43/88 (48.86%), Query Frame = 0

Query: 120 REKECGICMEV---------NGMVVLPNCNHSLCLKCYRDWRGRSQ-------SCPFCRD 179
           ++K CGICMEV         +   +L NCNH+ CLKC R WR   +       SCP CR 
Sbjct: 312 KDKVCGICMEVVYEKANPNEHRFGILSNCNHTFCLKCIRKWRSAKEFESRIVKSCPQCRI 371

Query: 180 SLRRVNSGDLWIYTDKAEMVDLTLIFQE 192
           +   V   + W+  +K E   L   ++E
Sbjct: 372 TSNFVIPSEYWV-EEKEEKQKLIQKYKE 398

BLAST of MS014904 vs. ExPASy Swiss-Prot
Match: Q9TT91 (E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 9.1e-07
Identity = 33/92 (35.87%), Postives = 44/92 (47.83%), Query Frame = 0

Query: 118 IEREKE--CGICMEV---------NGMVVLPNCNHSLCLKCYRDWRGRSQ-------SCP 177
           ++R K+  CGICMEV             +L NCNH+ CLKC R WR   Q       SCP
Sbjct: 269 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 328

Query: 178 FCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQE 192
            CR +   V   + W+  +K E   L   ++E
Sbjct: 329 ECRITSNFVIPSEYWV-EEKEEKQKLIQKYKE 359

BLAST of MS014904 vs. ExPASy Swiss-Prot
Match: Q4SRI6 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Tetraodon nigroviridis OX=99883 GN=mkrn1 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 9.1e-07
Identity = 33/96 (34.38%), Postives = 44/96 (45.83%), Query Frame = 0

Query: 118 IEREKE--CGICMEV---------NGMVVLPNCNHSLCLKCYRDWRGRSQ-------SCP 177
           I+R K+  CG+CMEV             +L NCNH  CLKC R WR   Q       SCP
Sbjct: 218 IQRSKDMMCGVCMEVVFEKANPSERRFGILSNCNHCYCLKCIRKWRSAKQFESKIIKSCP 277

Query: 178 FCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRKR 196
            CR +   V   + W+  DK +   L   +++   R
Sbjct: 278 ECRITSNFVIPSEYWV-EDKEDKQKLIQKYKDGMGR 312

BLAST of MS014904 vs. ExPASy TrEMBL
Match: A0A6J1DAW7 (uncharacterized protein LOC111018996 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018996 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.3e-125
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS
Sbjct: 24  ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA
Sbjct: 144 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 204 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 243

BLAST of MS014904 vs. ExPASy TrEMBL
Match: A0A6J1DBM8 (uncharacterized protein LOC111018996 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018996 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.3e-125
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS
Sbjct: 22  ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 81

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER
Sbjct: 82  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 141

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA
Sbjct: 142 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 201

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 202 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 241

BLAST of MS014904 vs. ExPASy TrEMBL
Match: A0A6J1D9Y6 (E3 ubiquitin-protein ligase rnf8 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111018996 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 9.4e-116
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 15  MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV 74
           MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV
Sbjct: 1   MRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMSIYERKASIREFYVV 60

Query: 75  IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV 134
           IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV
Sbjct: 61  IFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIEREKECGICMEVNGMV 120

Query: 135 VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK 194
           VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK
Sbjct: 121 VLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKAEMVDLTLIFQENRK 180

Query: 195 RLFIYIDKLPLVVPDSVFLPLDSHIR 221
           RLFIYIDKLPLVVPDSVFLPLDSHIR
Sbjct: 181 RLFIYIDKLPLVVPDSVFLPLDSHIR 206

BLAST of MS014904 vs. ExPASy TrEMBL
Match: A0A0A0LY84 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530140 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.1e-115
Identity = 199/220 (90.45%), Postives = 209/220 (95.00%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRE+DG CVQMRLSYSPAAPFFLFLVQWTDC LAGALGLLRILIYVTYPDGKTTMS
Sbjct: 24  ALDCPRESDGACVQMRLSYSPAAPFFLFLVQWTDCYLAGALGLLRILIYVTYPDGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQ+GITELEDRKQ+EVCNARY +RDE GRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQKGITELEDRKQKEVCNARYSRRDEFGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICME NGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSL+RVNSGDLWI+TDK+
Sbjct: 144 EKECGICMEFNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLKRVNSGDLWIFTDKS 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EM+DL  I  +NRKRLF YIDKLPLVVPD VFLP+DSHIR
Sbjct: 204 EMIDLNSILHDNRKRLFAYIDKLPLVVPDPVFLPMDSHIR 243

BLAST of MS014904 vs. ExPASy TrEMBL
Match: A0A6J1HMH6 (E3 ubiquitin-protein ligase rnf8-B-like OS=Cucurbita moschata OX=3662 GN=LOC111464952 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 199/220 (90.45%), Postives = 211/220 (95.91%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALDCPRE+DG CVQMRLSYSPAAPFFLFLVQWTDC LAGALGLLRILIYVTYPDGKTTM+
Sbjct: 24  ALDCPRESDGACVQMRLSYSPAAPFFLFLVQWTDCYLAGALGLLRILIYVTYPDGKTTMA 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           IYERKASIREFYVVIFPSLLQLQRGITELEDRKQ+EVCNARYK+RDE+GRGKLSEIDIER
Sbjct: 84  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQKEVCNARYKRRDEIGRGKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           EKECGICME+N MVVLPNCNHSLCL CYRDWR RSQSCPFCRDSL+RVNSGDLWI+TDK+
Sbjct: 144 EKECGICMEINTMVVLPNCNHSLCLMCYRDWRNRSQSCPFCRDSLKRVNSGDLWIFTDKS 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLPLDSHIR 221
           EM+DLT I Q+NRKRLF YI+KLPLVVPD VFLPLDSHIR
Sbjct: 204 EMIDLTSILQDNRKRLFAYIEKLPLVVPDPVFLPLDSHIR 243

BLAST of MS014904 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 6.3e-96
Identity = 160/222 (72.07%), Postives = 190/222 (85.59%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALD PRE DG  VQMRLSY+PAA F LFLVQWTDC LAG LGLLR+LIY+TY DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           +YERK SI++FY VIFPSLLQL+RGIT+L+DRKQ+EVC  RY+ +DE  + KLSEIDIER
Sbjct: 84  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGICME+N MVVLPNC HSLC+KCYRDW GRS+SCPFCRDSL+RVNSGDLW+  +K+
Sbjct: 144 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFL--PLDSHIR 221
           + V++  I +EN+KRLF+YI+KLPLVVPD VF   P D H++
Sbjct: 204 DTVNMYTIERENKKRLFVYIEKLPLVVPDQVFASSPYDCHVK 245

BLAST of MS014904 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 6.3e-96
Identity = 160/222 (72.07%), Postives = 190/222 (85.59%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALD PRE DG  VQMRLSY+PAA F LFLVQWTDC LAG LGLLR+LIY+TY DGKTTMS
Sbjct: 36  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 95

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           +YERK SI++FY VIFPSLLQL+RGIT+L+DRKQ+EVC  RY+ +DE  + KLSEIDIER
Sbjct: 96  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 155

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGICME+N MVVLPNC HSLC+KCYRDW GRS+SCPFCRDSL+RVNSGDLW+  +K+
Sbjct: 156 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 215

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFL--PLDSHIR 221
           + V++  I +EN+KRLF+YI+KLPLVVPD VF   P D H++
Sbjct: 216 DTVNMYTIERENKKRLFVYIEKLPLVVPDQVFASSPYDCHVK 257

BLAST of MS014904 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 2.9e-93
Identity = 159/222 (71.62%), Postives = 188/222 (84.68%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALD PRE DG  VQMRLSYSP A FFLFLVQWTDC LAG LGLLR+LIY+TY DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           +YERKASIREF  VI PSL QLQRG+T+++D KQ+EVC  RY+K+DE    ++SEI+IER
Sbjct: 84  VYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMRYRKKDE---SEMSEIEIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGICME+N  VVLPNC HSLC+KCYRDWRGRSQSCPFCRDSL+RV+SGDLW++ D+ 
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFL--PLDSHIR 221
           + V+LT I +EN+KRLF+YI+KLPLVVPD V+   P D H+R
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPDQVYASSPYDFHVR 242

BLAST of MS014904 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 8.2e-88
Identity = 148/214 (69.16%), Postives = 175/214 (81.78%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           A + P E DGG VQMRLSYSPAA  FLFL+QWTDC  AGALGLLRILIY  Y DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           ++ERK SIREFY V+FPSLLQL  GIT++E+RKQ+E+C+ RY+K+D   +GK+SEID+ER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGIC+E+   VVLP CNHS+C+ CYR+WR RSQSCPFCR SL+RVNSGDLWIYT  A
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFLP 215
           E+ DL  I++EN KRL IYIDKLPLV  D   +P
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVP 236

BLAST of MS014904 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 5.5e-84
Identity = 148/222 (66.67%), Postives = 176/222 (79.28%), Query Frame = 0

Query: 1   ALDCPRENDGGCVQMRLSYSPAAPFFLFLVQWTDCSLAGALGLLRILIYVTYPDGKTTMS 60
           ALD PRE DG  VQMRLSYSP A FFLFLVQWTDC LAG LGLLR+LIY+TY DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 61  IYERKASIREFYVVIFPSLLQLQRGITELEDRKQREVCNARYKKRDELGRGKLSEIDIER 120
           +YERKASIREF                +++D KQ+EVC  RY+K+DE    ++SEI+IER
Sbjct: 84  VYERKASIREF---------------QDIDDSKQKEVCKMRYRKKDE---SEMSEIEIER 143

Query: 121 EKECGICMEVNGMVVLPNCNHSLCLKCYRDWRGRSQSCPFCRDSLRRVNSGDLWIYTDKA 180
           E+ECGICME+N  VVLPNC HSLC+KCYRDWRGRSQSCPFCRDSL+RV+SGDLW++ D+ 
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 181 EMVDLTLIFQENRKRLFIYIDKLPLVVPDSVFL--PLDSHIR 221
           + V+LT I +EN+KRLF+YI+KLPLVVPD V+   P D H+R
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPDQVYASSPYDFHVR 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150973.12.7e-125100.00uncharacterized protein LOC111018996 isoform X1 [Momordica charantia][more]
XP_022150974.12.7e-125100.00uncharacterized protein LOC111018996 isoform X2 [Momordica charantia][more]
XP_022150975.12.0e-115100.00E3 ubiquitin-protein ligase rnf8 isoform X3 [Momordica charantia][more]
XP_038893737.14.3e-11590.45E3 ubiquitin-protein ligase AIRP2 [Benincasa hispida][more]
XP_011658560.14.3e-11590.45E3 ubiquitin-protein ligase AIRP2 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9M0221.2e-8669.16E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ... [more]
Q9UHC71.8e-0736.96E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3[more]
Q134345.3e-0735.23Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P... [more]
Q9TT919.1e-0735.87E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 ... [more]
Q4SRI69.1e-0734.38Probable E3 ubiquitin-protein ligase makorin-1 OS=Tetraodon nigroviridis OX=9988... [more]
Match NameE-valueIdentityDescription
A0A6J1DAW71.3e-125100.00uncharacterized protein LOC111018996 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DBM81.3e-125100.00uncharacterized protein LOC111018996 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D9Y69.4e-116100.00E3 ubiquitin-protein ligase rnf8 isoform X3 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0LY842.1e-11590.45RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530140 P... [more]
A0A6J1HMH62.7e-11590.45E3 ubiquitin-protein ligase rnf8-B-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G47160.16.3e-9672.07RING/U-box superfamily protein [more]
AT3G47160.26.3e-9672.07RING/U-box superfamily protein [more]
AT5G58787.12.9e-9371.62RING/U-box superfamily protein [more]
AT5G01520.18.2e-8869.16RING/U-box superfamily protein [more]
AT5G58787.25.5e-8466.67RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 124..161
e-value: 1.6E-7
score: 41.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 124..162
score: 11.766381
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 111..171
e-value: 7.6E-14
score: 52.7
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 121..167
e-value: 1.4E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR15315:SF75RING/U-BOX SUPERFAMILY PROTEINcoord: 1..220
NoneNo IPR availablePANTHERPTHR15315RING FINGER PROTEIN 41, 151coord: 1..220
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 111..166
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 139..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014904.1MS014904.1mRNA