MS014810 (gene) Bitter gourd (TR) v1

Overview
NameMS014810
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY protein
Locationscaffold493: 426582 .. 428214 (+)
RNA-Seq ExpressionMS014810
SyntenyMS014810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAGGTTGAAGAAGCTAATAAAGCGGCCGTGGAGAGCTGCCACAGAGTGCTGAATCTGCTGTCTACGCCGCCGCAAGATCAGTCGAAGCTCAGAAGTTTAATGGCGGAAACAGGGGAGGCTGTGTTCAAGTTTAGGAAAGTTGTGTCGCTTCTGAATTCTTCTGGGTTGGGTCATGCAAGGGTTAGGAAGCAGCTCAAGAAGTTTCAGTTTCAGTTTCAGTTTCAGTCTCAGTCTCAGTCTTCCCCTTTTCTTCCCCACACCATGTTGTTGGAAAATCCCCCAAATTGCAGAACGGACCTCCTCCAGGGGAAGAATTTGCAGATGGGTCCTCTCTGTTTGAGCTCAAATGGGAAGAGTTCTCTCCAACAAGCTCAATCTGCTTCTGCCCACTATCACCACTTCCTTCAGCAGCAGCAGCAGACTAGGGTGTTGCTTCAGAATAATCCACAGGCTGAAATGATGTATCATCTTAGGGGTAATAATAGTGGGATCAACTTGAATTTTGATAGCTCTAGCTGCACACACACAATGTCATCAACTAGGTCCTTCATTTCCTCCTTGAGCATTGATGGGAGTGTGGCGAATTTGGATGGGAGTGGCGCGTTCCATTTGATCGGGGCGCCCCGGTCCTCGGATCAGAATTCCCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTAAAATGTGGAAGCAGTGGAAGATGTCACTGCTCAAAGAAGAGGTTGGGAAGCTGTGGTTTTGATATCGGAATGGGGACGGTTTGATACGGTTTTCTGATTCGATTTTTTCGATGATGTTCTTGCAGGAAACATAGAGTGAAGCGGTCGATTAAGGTGCCTGCGATAAGTAACAAGCTTGCGGATATCCCTCCTGATGATTATTCATGGAGGAAATATGGCCAGAAGCCAATCAAGGGATCTCCTCACCCGAGGTATAGGACTATTCTTTCGCGCCTTTTCAGCTTATCATGATGCTTATTTCAATCTAGATTGATAGGTTTTCGCAAATGTAATTAGCTGATTAATTATATGGATTGATTGGGGGAGGGGGAGGTTGTATAATTGGCATCAATGAAATATTTGCACTCTTACTTTTGGTTTTGACATTACTTTATGGATTGAAATATCTCTTGCCATTTCAATTATAGTAATGTAGTTGGGATATTGAAAACTTGCTTTGTTTATTTACTCGTGTGCTCGGAATAGAGTACGCCGTTGAACTCTCCACCTGGGGGTAGTCCATTTTGTGGCGTTTATAGAAAAGGAATTAGTGTTGTTCATTTCACATATTGTATGATTTTGGCAAATTGTTACGAGGCTCCCATGTATCTCATTCTGCTAAATACTTAACACAGTTACTGTAATTTCAACCTGAAATTCAAGAGGGACAGGAGTTCCATGAATGCATCAATGATTATATATCATATAAAATTTGTTCCTTCATGTCGATCTTAATCTACTTTCGAACATCTGCAGGGGATACTATAAGTGCAGCAGCATGAGAGGTTGTCCAGCGAGGAAGCACGTCGAGAGGTGTCTTGAGGAACCTTCAATGCTCATTGTAACATATGAAGGTGAACACAACCATCCCAGAATACCTTCTCAATCTGCAAATACA

mRNA sequence

ATGGAAGAGGTTGAAGAAGCTAATAAAGCGGCCGTGGAGAGCTGCCACAGAGTGCTGAATCTGCTGTCTACGCCGCCGCAAGATCAGTCGAAGCTCAGAAGTTTAATGGCGGAAACAGGGGAGGCTGTGTTCAAGTTTAGGAAAGTTGTGTCGCTTCTGAATTCTTCTGGGTTGGGTCATGCAAGGGTTAGGAAGCAGCTCAAGAAGTTTCAGTTTCAGTTTCAGTTTCAGTCTCAGTCTCAGTCTTCCCCTTTTCTTCCCCACACCATGTTGTTGGAAAATCCCCCAAATTGCAGAACGGACCTCCTCCAGGGGAAGAATTTGCAGATGGGTCCTCTCTGTTTGAGCTCAAATGGGAAGAGTTCTCTCCAACAAGCTCAATCTGCTTCTGCCCACTATCACCACTTCCTTCAGCAGCAGCAGCAGACTAGGGTGTTGCTTCAGAATAATCCACAGGCTGAAATGATGTATCATCTTAGGGGTAATAATAGTGGGATCAACTTGAATTTTGATAGCTCTAGCTGCACACACACAATGTCATCAACTAGGTCCTTCATTTCCTCCTTGAGCATTGATGGGAGTGTGGCGAATTTGGATGGGAGTGGCGCGTTCCATTTGATCGGGGCGCCCCGGTCCTCGGATCAGAATTCCCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTAAAATGTGGAAGCAGTGGAAGATGTCACTGCTCAAAGAAGAGGAAACATAGAGTGAAGCGGTCGATTAAGGTGCCTGCGATAAGTAACAAGCTTGCGGATATCCCTCCTGATGATTATTCATGGAGGAAATATGGCCAGAAGCCAATCAAGGGATCTCCTCACCCGAGGGGATACTATAAGTGCAGCAGCATGAGAGGTTGTCCAGCGAGGAAGCACGTCGAGAGGTGTCTTGAGGAACCTTCAATGCTCATTGTAACATATGAAGGTGAACACAACCATCCCAGAATACCTTCTCAATCTGCAAATACA

Coding sequence (CDS)

ATGGAAGAGGTTGAAGAAGCTAATAAAGCGGCCGTGGAGAGCTGCCACAGAGTGCTGAATCTGCTGTCTACGCCGCCGCAAGATCAGTCGAAGCTCAGAAGTTTAATGGCGGAAACAGGGGAGGCTGTGTTCAAGTTTAGGAAAGTTGTGTCGCTTCTGAATTCTTCTGGGTTGGGTCATGCAAGGGTTAGGAAGCAGCTCAAGAAGTTTCAGTTTCAGTTTCAGTTTCAGTCTCAGTCTCAGTCTTCCCCTTTTCTTCCCCACACCATGTTGTTGGAAAATCCCCCAAATTGCAGAACGGACCTCCTCCAGGGGAAGAATTTGCAGATGGGTCCTCTCTGTTTGAGCTCAAATGGGAAGAGTTCTCTCCAACAAGCTCAATCTGCTTCTGCCCACTATCACCACTTCCTTCAGCAGCAGCAGCAGACTAGGGTGTTGCTTCAGAATAATCCACAGGCTGAAATGATGTATCATCTTAGGGGTAATAATAGTGGGATCAACTTGAATTTTGATAGCTCTAGCTGCACACACACAATGTCATCAACTAGGTCCTTCATTTCCTCCTTGAGCATTGATGGGAGTGTGGCGAATTTGGATGGGAGTGGCGCGTTCCATTTGATCGGGGCGCCCCGGTCCTCGGATCAGAATTCCCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTAAAATGTGGAAGCAGTGGAAGATGTCACTGCTCAAAGAAGAGGAAACATAGAGTGAAGCGGTCGATTAAGGTGCCTGCGATAAGTAACAAGCTTGCGGATATCCCTCCTGATGATTATTCATGGAGGAAATATGGCCAGAAGCCAATCAAGGGATCTCCTCACCCGAGGGGATACTATAAGTGCAGCAGCATGAGAGGTTGTCCAGCGAGGAAGCACGTCGAGAGGTGTCTTGAGGAACCTTCAATGCTCATTGTAACATATGAAGGTGAACACAACCATCCCAGAATACCTTCTCAATCTGCAAATACA

Protein sequence

MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGHARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQGKNLQMGPLCLSSNGKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT
Homology
BLAST of MS014810 vs. NCBI nr
Match: XP_022155836.1 (probable WRKY transcription factor 21 [Momordica charantia])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 334/337 (99.11%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH
Sbjct: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60

Query: 61  ARVRKQLKK--FQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQGKNLQMGPLCLSSN 120
           ARVRKQLKK  FQFQFQFQSQSQSSPFLP TMLLENPPNCRTDLLQGKNLQMGPLCLSSN
Sbjct: 61  ARVRKQLKKFQFQFQFQFQSQSQSSPFLPRTMLLENPPNCRTDLLQGKNLQMGPLCLSSN 120

Query: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT 180
           GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT
Sbjct: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT 180

Query: 181 MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG 240
           MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG
Sbjct: 181 MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG 240

Query: 241 RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300
           RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG
Sbjct: 241 RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300

Query: 301 CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT
Sbjct: 301 CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 337

BLAST of MS014810 vs. NCBI nr
Match: AWB50945.1 (WRKY transcription factor 21-like protein [Luffa aegyptiaca])

HSP 1 Score: 495.4 bits (1274), Expect = 4.0e-136
Identity = 283/361 (78.39%), Postives = 299/361 (82.83%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLS-TPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSGL 60
           MEEVEEAN+ AVESCHRVLNLL+   PQD SKLRS LMAETG+AVFKFRKV+ LL+SSGL
Sbjct: 1   MEEVEEANRDAVESCHRVLNLLTVASPQDHSKLRSCLMAETGQAVFKFRKVLCLLDSSGL 60

Query: 61  GHARVR-KQLKKFQFQFQFQSQSQSSPF-LPHTMLLEN-PPNCRTDLLQGKNLQMGPLC- 120
           GHARVR K++K FQF       + SSPF LP +M LE   PNCR DLLQG+NLQMGPL  
Sbjct: 61  GHARVRKKKVKSFQF-------NSSSPFPLPQSMFLETFSPNCRMDLLQGRNLQMGPLSL 120

Query: 121 ------LSSNGKS------SLQQAQSASAHYHHFLQQQQQTRVLLQNNP-------QAEM 180
                 LSSNGKS        QQ QS   ++HHFL    Q RVLL +NP       Q EM
Sbjct: 121 GSPSLELSSNGKSCSIQQQQQQQPQSVGHYHHHFL----QNRVLLNSNPNINPNASQPEM 180

Query: 181 MYHLRGNNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSD 240
           +Y LR +NSGINLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSD
Sbjct: 181 VY-LR-SNSGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300
           QNSHHKRKCSGRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR
Sbjct: 241 QNSHHKRKCSGRGEDGSVKCGSTGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN 336
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN 347

BLAST of MS014810 vs. NCBI nr
Match: XP_038899952.1 (probable WRKY transcription factor 74 isoform X1 [Benincasa hispida])

HSP 1 Score: 487.3 bits (1253), Expect = 1.1e-133
Identity = 279/356 (78.37%), Postives = 294/356 (82.58%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLL--STPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVEEANK AVESCH+VLNLL  ++  QDQ KLRS LMAETGEAVFKFRKV+ LL+SSG
Sbjct: 1   MEEVEEANKEAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 61  LGHARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLE-NPPNCRTDLLQGKNLQMGPLC-- 120
           LGHARVRK  KK    F F S S   P LP +M LE + PNCR DLLQG+NLQ GPLC  
Sbjct: 61  LGHARVRK--KKVVKNFLFNSSSSHFP-LPQSMFLETSSPNCRMDLLQGRNLQTGPLCLG 120

Query: 121 -----LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN-------PQAEMMYHLR 180
                L+ NGKS S+QQ QS SA  YHH L    Q R LL NN       PQ EM+Y LR
Sbjct: 121 KPSLELNPNGKSCSIQQIQSQSAALYHHHL---LQNRALLNNNPNPNPNPPQPEMVY-LR 180

Query: 181 GNNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHH 240
            N++GINLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+H
Sbjct: 181 SNSNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYH 240

Query: 241 KRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQK 300
           KRKCSGRGEDGSVKCGSSG+CHCSKKRKHRVKRSIKVPAISNKLADIPPD YSWRKYGQK
Sbjct: 241 KRKCSGRGEDGSVKCGSSGKCHCSKKRKHRVKRSIKVPAISNKLADIPPDYYSWRKYGQK 300

Query: 301 PIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           PIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 301 PIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 348

BLAST of MS014810 vs. NCBI nr
Match: KAA0059712.1 (putative WRKY transcription factor 21 [Cucumis melo var. makuwa] >TYK26150.1 putative WRKY transcription factor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 483.0 bits (1242), Expect = 2.0e-132
Identity = 275/355 (77.46%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLL--STPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVE+AN+ AVESCH+VLNLL  ++  QDQ KLRS LMAETGEA+FKFRKV+ LL+SSG
Sbjct: 2   MEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSSG 61

Query: 61  LGHARVRKQLK--KFQFQFQFQSQSQSSPF-LPHTMLLE-NPPNCRTDLLQGKNLQMGPL 120
           LGHARVRK+ K   F F     S S SSPF LP ++ LE + P+CR DLLQ +NLQMGPL
Sbjct: 62  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLQMGPL 121

Query: 121 C-------LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN---PQAEMMYHLRG 180
           C       L++N KS S+QQ QS SA  YHH L    Q RV+L NN   PQ EM+Y LR 
Sbjct: 122 CLGNPSLELNTNAKSCSIQQIQSQSAALYHHHL---LQNRVVLNNNPNPPQHEMVY-LRS 181

Query: 181 NNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           NN G+NLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+HK
Sbjct: 182 NN-GVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYHK 241

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           RKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP
Sbjct: 242 RKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 301

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 302 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 350

BLAST of MS014810 vs. NCBI nr
Match: XP_004146948.2 (probable WRKY transcription factor 21 [Cucumis sativus] >ADU52528.1 WRKY protein [Cucumis sativus])

HSP 1 Score: 482.6 bits (1241), Expect = 2.7e-132
Identity = 273/355 (76.90%), Postives = 295/355 (83.10%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTP--PQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVEEAN+ AVESCH+VLNLL+ P   QD  KLRS LMAETGEAVFKFRKV+ LL+SSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 61  LGHARVRKQLK--KFQFQFQFQSQSQSSPF-LPHTMLLEN-PPNCRTDLLQGKNLQMGPL 120
           LGHARVRK+ K   F F     S S SSPF LP ++ LE   P+CR D LQG+NLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 121 C-------LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN---PQAEMMYHLRG 180
           C       L++N K+ S+QQ QS SA  YHH      Q RV+L NN   PQ E++Y LR 
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALYHHHHHHLLQNRVVLNNNPNPPQPEVVY-LRS 180

Query: 181 NNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           +N G+NLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+HK
Sbjct: 181 SN-GVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYHK 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           RKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP
Sbjct: 241 RKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 352

BLAST of MS014810 vs. ExPASy Swiss-Prot
Match: O04336 (Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 PE=1 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.2e-88
Identity = 205/384 (53.39%), Postives = 242/384 (63.02%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQ--SKLRSLMAETGEAVFKFRKVVSLLNSSGL 60
           MEE+E  N+AAVESCHRVLNLL    Q       ++L++ET EAV +F++V SLL+SS +
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLHRSQQQDHVGFEKNLVSETREAVIRFKRVGSLLSSS-V 60

Query: 61  GHARVRKQLKKFQFQFQF--------------QSQSQSSPFLP---HTMLLENP------ 120
           GHAR R+  K      Q                S SQ +P L      + L  P      
Sbjct: 61  GHARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLTL 120

Query: 121 --------PNCRTDLLQGKNLQMG----PLCLSSNGKSSLQQAQSASAHYHHFLQQQQQT 180
                    N +  LLQ     M     P       +   QQ Q          QQQQQ 
Sbjct: 121 GTRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQF 180

Query: 181 RVLLQ----------NNPQAEMMYHLRGNNSGINLNFDSSSCTHTMSSTRSFISSLSIDG 240
              LQ             QAE+M  LR  N GI+L+FD+SSCT TMSSTRSF+SSLSIDG
Sbjct: 181 HERLQAHHLHQQQQLQKHQAELM--LRKCNGGISLSFDNSSCTPTMSSTRSFVSSLSIDG 240

Query: 241 SVANLDGSGAFHLIGAPRSSDQNS-HHKRKCSGRG-EDGSVKCGSSGRCHCSKKRKHRVK 300
           SVAN++G  +FH  G P S+DQNS H KRKC  +G E GS+KCGSS RCHC+KKRKHRV+
Sbjct: 241 SVANIEGKNSFH-FGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCHCAKKRKHRVR 300

Query: 301 RSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEE 336
           RSI+VPAISNK+ADIPPDDYSWRKYGQKPIKGSP+PRGYYKCSSMRGCPARKHVERCLE+
Sbjct: 301 RSIRVPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPARKHVERCLED 360

BLAST of MS014810 vs. ExPASy Swiss-Prot
Match: Q93WU6 (Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 PE=2 SV=2)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 188/347 (54.18%), Postives = 224/347 (64.55%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVE ANKAAVESCH VLNLLS    D    +S+M ET EAV KF++V SLL S GLG 
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLSQQTNDS---KSIMVETREAVCKFKRVSSLL-SRGLGQ 60

Query: 61  ARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQG--KNLQMGPLCLSSN 120
            +++K L    ++F       SS  LP  M LE+P  C  + + G    L   PL +   
Sbjct: 61  RKIKK-LNNNNYKF-------SSSLLPQHMFLESPV-CSNNAISGCIPILAPKPLQIVPA 120

Query: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRG----NNSGINLNFDSS- 180
           G   L            FL+ +  +   +   P   +  H +G    + SG+NL FD S 
Sbjct: 121 GPPPLMLFNQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQQGVYSRSKSGLNLKFDGSI 180

Query: 181 --SC--THTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGED 240
             SC      + +RSF+SSLS+DGSV + D   +FHLIG P+ SD  S H R+ S     
Sbjct: 181 GASCYSPSISNGSRSFVSSLSMDGSVTDYD-RNSFHLIGLPQGSDHISQHSRRTSC---S 240

Query: 241 GSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRG 300
           GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD+YSWRKYGQKPIKGSPHPRG
Sbjct: 241 GSLKCGSKSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRG 300

Query: 301 YYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRI-PSQSANT 336
           YYKCSS+RGCPARKHVERC+EE SMLIVTYEGEHNH RI  SQSA+T
Sbjct: 301 YYKCSSVRGCPARKHVERCVEETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of MS014810 vs. ExPASy Swiss-Prot
Match: Q9SR07 (Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.1e-75
Identity = 188/356 (52.81%), Postives = 221/356 (62.08%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVE AN++A+ESCH VLNLLS   Q  S  +SL  ETGE V KF++V SLL + GLGH
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLS---QRTSDPKSLTVETGEVVSKFKRVASLL-TRGLGH 60

Query: 61  ARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDL-------LQGKNLQMGPL 120
            + R    KF+  F            P  + LE+P  C  DL       L  + LQM P 
Sbjct: 61  GKFR-STNKFRSSF------------PQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPA 120

Query: 121 CLSSNGKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGN---------NS 180
               N      Q    S    H +   +    L    P     Y L  N         NS
Sbjct: 121 SAVYNEMEPKHQLGHPSLMLSHKMCVDKS--FLELKPPPFRAPYQLIHNHQQIAYSRSNS 180

Query: 181 GINLNFD--SSSC-THTMSS-TRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           G+NL FD   SSC T ++S+ +RSF+SSLS+D SV + D   +FHL G  R SDQ  H +
Sbjct: 181 GVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYD-RNSFHLTGLSRGSDQ-QHTR 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           + CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD+YSWRKYGQKP
Sbjct: 241 KMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKP 300

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRI-PSQSANT 336
           IKGSPHPRGYYKCSS+RGCPARKHVERC++E SMLIVTYEGEHNH RI  SQSA+T
Sbjct: 301 IKGSPHPRGYYKCSSVRGCPARKHVERCIDETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of MS014810 vs. ExPASy Swiss-Prot
Match: Q32SG4 (Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.5e-72
Identity = 182/404 (45.05%), Postives = 232/404 (57.43%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLL---NSSG 60
           ME +EEAN+ AVESCHRVL LLS P       + L+A TGEAV KF  + + L   N  G
Sbjct: 1   MEGMEEANRTAVESCHRVLALLSNPHGQLVPSKELVAATGEAVAKFGSLTAKLSNSNGDG 60

Query: 61  L--GHARVRK-----------------------------------QLKKFQFQFQFQSQS 120
           L  GHARVRK                                    L+ F    QF+  S
Sbjct: 61  LLQGHARVRKVKKPLHIFDSNLFLESSAVAAAAAPAKTPSPSPILGLQLFPRYHQFEGSS 120

Query: 121 QSSPF-----LPHTMLLENPPNCRTDLLQGKNLQMGPLCLSSNGKSSLQQAQSAS----- 180
              P       P  +LLE P    T  ++G   Q  P+       S    A + +     
Sbjct: 121 SKDPVRIPTQFPKRLLLEKP----TAGMEGSTSQSPPIVQMVQPVSVAPPAGTPTPALPP 180

Query: 181 AHYHHFLQQQ--QQTRVLLQNNPQAEMMYHLR-------------GNNSGINLNFDSSSC 240
           AH H   QQQ  Q+ +++ Q   Q+EMM                 G   G+NL FDSS+C
Sbjct: 181 AHLHFIQQQQSYQRFQLMQQMKIQSEMMKRSNLGDQGGSLSGGGGGGRKGVNLKFDSSNC 240

Query: 241 THTMSSTRSFISSLSIDGSVANLDGSGA---FHLIGAPRSS---DQNSHHKRKCSGRGED 300
             T SS+RSF+SSLS++GS+A+LDGS     F L+   +++   +     +R+C+GR ED
Sbjct: 241 --TASSSRSFLSSLSMEGSLASLDGSRTSRPFQLLSGSQTASTPELGLVQRRRCAGR-ED 300

Query: 301 GSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRG 334
           G+ +C +  RCHCSKKRK R++RSIKVPAISNK+ADIP D++SWRKYGQKPIKGSPHPRG
Sbjct: 301 GTGRCATGSRCHCSKKRKLRIRRSIKVPAISNKVADIPADEFSWRKYGQKPIKGSPHPRG 360

BLAST of MS014810 vs. ExPASy Swiss-Prot
Match: Q0JEE2 (WRKY transcription factor WRKY51 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.4e-43
Identity = 129/350 (36.86%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 4   VEEANKAAVESC-HRVLNLLST--------PPQDQSKLRSLMAETGEAVFKFRKVVSLLN 63
           ++EA  A +    H +L L  T        P Q+Q +       T   V KF+KV+S+LN
Sbjct: 19  IQEAAAAGLRGMEHLILQLSQTGTSERSPAPAQEQQQQVDCREITDMTVSKFKKVISMLN 78

Query: 64  SSGLGHARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQGKNLQMGPLC 123
            +  GHAR R+                                             GP+ 
Sbjct: 79  RT--GHARFRR---------------------------------------------GPVV 138

Query: 124 LSSNGKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSS 183
             S+G ++ + A   S+           +R +  +  +A   Y   G ++G +++  S  
Sbjct: 139 AQSSGPAASEPAPVRSS-------PSAVSRPMTLDFTKAASGY---GKDAGFSVSGIS-- 198

Query: 184 CTHTMSSTRSFISSLSIDGSVANLDGSGAFHLI---------------GAPRSSDQNSHH 243
                +++ SF+SS++ DGSV+N  G G+  L+                A  +    + H
Sbjct: 199 -----AASSSFLSSVTGDGSVSNGRGGGSSSLMLPPPPATSCGKPPLSSAAAAMSAGAGH 258

Query: 244 KRKCSGRGEDGSVKCG----SSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRK 303
           KRKC       +V  G    + GRCHCSK+RKHRVKR+I+VPAIS+K+ADIP DD+SWRK
Sbjct: 259 KRKCHDHAHSENVAGGKYGSTGGRCHCSKRRKHRVKRTIRVPAISSKVADIPADDFSWRK 304

Query: 304 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNH 326
           YGQKPIKGSP PRGYYKCS++RGCPARKHVER   +PSMLIVTYEGEH H
Sbjct: 319 YGQKPIKGSPFPRGYYKCSTLRGCPARKHVERDPTDPSMLIVTYEGEHRH 304

BLAST of MS014810 vs. ExPASy TrEMBL
Match: A0A6J1DSX4 (probable WRKY transcription factor 21 OS=Momordica charantia OX=3673 GN=LOC111022865 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.9e-186
Identity = 334/337 (99.11%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH
Sbjct: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60

Query: 61  ARVRKQLKK--FQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQGKNLQMGPLCLSSN 120
           ARVRKQLKK  FQFQFQFQSQSQSSPFLP TMLLENPPNCRTDLLQGKNLQMGPLCLSSN
Sbjct: 61  ARVRKQLKKFQFQFQFQFQSQSQSSPFLPRTMLLENPPNCRTDLLQGKNLQMGPLCLSSN 120

Query: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT 180
           GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT
Sbjct: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHT 180

Query: 181 MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG 240
           MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG
Sbjct: 181 MSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSG 240

Query: 241 RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300
           RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG
Sbjct: 241 RCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRG 300

Query: 301 CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT
Sbjct: 301 CPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 337

BLAST of MS014810 vs. ExPASy TrEMBL
Match: A0A2S0USY9 (WRKY transcription factor 21-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.9e-136
Identity = 283/361 (78.39%), Postives = 299/361 (82.83%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLS-TPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSGL 60
           MEEVEEAN+ AVESCHRVLNLL+   PQD SKLRS LMAETG+AVFKFRKV+ LL+SSGL
Sbjct: 1   MEEVEEANRDAVESCHRVLNLLTVASPQDHSKLRSCLMAETGQAVFKFRKVLCLLDSSGL 60

Query: 61  GHARVR-KQLKKFQFQFQFQSQSQSSPF-LPHTMLLEN-PPNCRTDLLQGKNLQMGPLC- 120
           GHARVR K++K FQF       + SSPF LP +M LE   PNCR DLLQG+NLQMGPL  
Sbjct: 61  GHARVRKKKVKSFQF-------NSSSPFPLPQSMFLETFSPNCRMDLLQGRNLQMGPLSL 120

Query: 121 ------LSSNGKS------SLQQAQSASAHYHHFLQQQQQTRVLLQNNP-------QAEM 180
                 LSSNGKS        QQ QS   ++HHFL    Q RVLL +NP       Q EM
Sbjct: 121 GSPSLELSSNGKSCSIQQQQQQQPQSVGHYHHHFL----QNRVLLNSNPNINPNASQPEM 180

Query: 181 MYHLRGNNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSD 240
           +Y LR +NSGINLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSD
Sbjct: 181 VY-LR-SNSGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300
           QNSHHKRKCSGRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR
Sbjct: 241 QNSHHKRKCSGRGEDGSVKCGSTGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN 336
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSAN 347

BLAST of MS014810 vs. ExPASy TrEMBL
Match: A0A5D3DSD0 (Putative WRKY transcription factor 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00720 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 9.9e-133
Identity = 275/355 (77.46%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLL--STPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVE+AN+ AVESCH+VLNLL  ++  QDQ KLRS LMAETGEA+FKFRKV+ LL+SSG
Sbjct: 2   MEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSSG 61

Query: 61  LGHARVRKQLK--KFQFQFQFQSQSQSSPF-LPHTMLLE-NPPNCRTDLLQGKNLQMGPL 120
           LGHARVRK+ K   F F     S S SSPF LP ++ LE + P+CR DLLQ +NLQMGPL
Sbjct: 62  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLQMGPL 121

Query: 121 C-------LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN---PQAEMMYHLRG 180
           C       L++N KS S+QQ QS SA  YHH L    Q RV+L NN   PQ EM+Y LR 
Sbjct: 122 CLGNPSLELNTNAKSCSIQQIQSQSAALYHHHL---LQNRVVLNNNPNPPQHEMVY-LRS 181

Query: 181 NNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           NN G+NLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+HK
Sbjct: 182 NN-GVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYHK 241

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           RKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP
Sbjct: 242 RKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 301

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 302 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 350

BLAST of MS014810 vs. ExPASy TrEMBL
Match: E7CEY5 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY51 PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.3e-132
Identity = 273/355 (76.90%), Postives = 295/355 (83.10%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTP--PQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVEEAN+ AVESCH+VLNLL+ P   QD  KLRS LMAETGEAVFKFRKV+ LL+SSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 61  LGHARVRKQLK--KFQFQFQFQSQSQSSPF-LPHTMLLEN-PPNCRTDLLQGKNLQMGPL 120
           LGHARVRK+ K   F F     S S SSPF LP ++ LE   P+CR D LQG+NLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 121 C-------LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN---PQAEMMYHLRG 180
           C       L++N K+ S+QQ QS SA  YHH      Q RV+L NN   PQ E++Y LR 
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALYHHHHHHLLQNRVVLNNNPNPPQPEVVY-LRS 180

Query: 181 NNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           +N G+NLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+HK
Sbjct: 181 SN-GVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYHK 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           RKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP
Sbjct: 241 RKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 352

BLAST of MS014810 vs. ExPASy TrEMBL
Match: N1K0K3 (WRKY transcription factor 21 OS=Cucumis sativus OX=3659 GN=WRKY-21 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.8e-132
Identity = 272/355 (76.62%), Postives = 294/355 (82.82%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTP--PQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSG 60
           MEEVEEAN+ AVESCH+VLNLL+ P   QD  KLRS LMAETGEA FKFRKV+ LL+SSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAAFKFRKVLCLLDSSG 60

Query: 61  LGHARVRKQLK--KFQFQFQFQSQSQSSPF-LPHTMLLEN-PPNCRTDLLQGKNLQMGPL 120
           LGHARVRK+ K   F F     S S SSPF LP ++ LE   P+CR D LQG+NLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 121 C-------LSSNGKS-SLQQAQSASAH-YHHFLQQQQQTRVLLQNN---PQAEMMYHLRG 180
           C       L++N K+ S+QQ QS SA  YHH      Q RV+L NN   PQ E++Y LR 
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALYHHHHHHLLQNRVVLNNNPNPPQPEVVY-LRS 180

Query: 181 NNSGINLNFDSSSCT-HTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           +N G+NLNFDSSSCT HTMSSTRSFISSLSIDGSVANLDGS AFHLIGAPRSSDQNS+HK
Sbjct: 181 SN-GVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGS-AFHLIGAPRSSDQNSYHK 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           RKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP
Sbjct: 241 RKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQSANT 336
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQ ANT
Sbjct: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRIPSQPANT 352

BLAST of MS014810 vs. TAIR 10
Match: AT2G30590.1 (WRKY DNA-binding protein 21 )

HSP 1 Score: 326.6 bits (836), Expect = 2.3e-89
Identity = 205/384 (53.39%), Postives = 242/384 (63.02%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQ--SKLRSLMAETGEAVFKFRKVVSLLNSSGL 60
           MEE+E  N+AAVESCHRVLNLL    Q       ++L++ET EAV +F++V SLL+SS +
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLHRSQQQDHVGFEKNLVSETREAVIRFKRVGSLLSSS-V 60

Query: 61  GHARVRKQLKKFQFQFQF--------------QSQSQSSPFLP---HTMLLENP------ 120
           GHAR R+  K      Q                S SQ +P L      + L  P      
Sbjct: 61  GHARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLTL 120

Query: 121 --------PNCRTDLLQGKNLQMG----PLCLSSNGKSSLQQAQSASAHYHHFLQQQQQT 180
                    N +  LLQ     M     P       +   QQ Q          QQQQQ 
Sbjct: 121 GTRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQF 180

Query: 181 RVLLQ----------NNPQAEMMYHLRGNNSGINLNFDSSSCTHTMSSTRSFISSLSIDG 240
              LQ             QAE+M  LR  N GI+L+FD+SSCT TMSSTRSF+SSLSIDG
Sbjct: 181 HERLQAHHLHQQQQLQKHQAELM--LRKCNGGISLSFDNSSCTPTMSSTRSFVSSLSIDG 240

Query: 241 SVANLDGSGAFHLIGAPRSSDQNS-HHKRKCSGRG-EDGSVKCGSSGRCHCSKKRKHRVK 300
           SVAN++G  +FH  G P S+DQNS H KRKC  +G E GS+KCGSS RCHC+KKRKHRV+
Sbjct: 241 SVANIEGKNSFH-FGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCHCAKKRKHRVR 300

Query: 301 RSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEE 336
           RSI+VPAISNK+ADIPPDDYSWRKYGQKPIKGSP+PRGYYKCSSMRGCPARKHVERCLE+
Sbjct: 301 RSIRVPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPARKHVERCLED 360

BLAST of MS014810 vs. TAIR 10
Match: AT5G28650.1 (WRKY DNA-binding protein 74 )

HSP 1 Score: 294.7 bits (753), Expect = 9.6e-80
Identity = 188/347 (54.18%), Postives = 224/347 (64.55%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVE ANKAAVESCH VLNLLS    D    +S+M ET EAV KF++V SLL S GLG 
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLSQQTNDS---KSIMVETREAVCKFKRVSSLL-SRGLGQ 60

Query: 61  ARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQG--KNLQMGPLCLSSN 120
            +++K L    ++F       SS  LP  M LE+P  C  + + G    L   PL +   
Sbjct: 61  RKIKK-LNNNNYKF-------SSSLLPQHMFLESPV-CSNNAISGCIPILAPKPLQIVPA 120

Query: 121 GKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRG----NNSGINLNFDSS- 180
           G   L            FL+ +  +   +   P   +  H +G    + SG+NL FD S 
Sbjct: 121 GPPPLMLFNQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQQGVYSRSKSGLNLKFDGSI 180

Query: 181 --SC--THTMSSTRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGED 240
             SC      + +RSF+SSLS+DGSV + D   +FHLIG P+ SD  S H R+ S     
Sbjct: 181 GASCYSPSISNGSRSFVSSLSMDGSVTDYD-RNSFHLIGLPQGSDHISQHSRRTSC---S 240

Query: 241 GSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRG 300
           GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD+YSWRKYGQKPIKGSPHPRG
Sbjct: 241 GSLKCGSKSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRG 300

Query: 301 YYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRI-PSQSANT 336
           YYKCSS+RGCPARKHVERC+EE SMLIVTYEGEHNH RI  SQSA+T
Sbjct: 301 YYKCSSVRGCPARKHVERCVEETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of MS014810 vs. TAIR 10
Match: AT3G04670.1 (WRKY DNA-binding protein 39 )

HSP 1 Score: 282.0 bits (720), Expect = 6.5e-76
Identity = 188/356 (52.81%), Postives = 221/356 (62.08%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVE AN++A+ESCH VLNLLS   Q  S  +SL  ETGE V KF++V SLL + GLGH
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLS---QRTSDPKSLTVETGEVVSKFKRVASLL-TRGLGH 60

Query: 61  ARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDL-------LQGKNLQMGPL 120
            + R    KF+  F            P  + LE+P  C  DL       L  + LQM P 
Sbjct: 61  GKFR-STNKFRSSF------------PQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPA 120

Query: 121 CLSSNGKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGN---------NS 180
               N      Q    S    H +   +    L    P     Y L  N         NS
Sbjct: 121 SAVYNEMEPKHQLGHPSLMLSHKMCVDKS--FLELKPPPFRAPYQLIHNHQQIAYSRSNS 180

Query: 181 GINLNFD--SSSC-THTMSS-TRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           G+NL FD   SSC T ++S+ +RSF+SSLS+D SV + D   +FHL G  R SDQ  H +
Sbjct: 181 GVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYD-RNSFHLTGLSRGSDQ-QHTR 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 300
           + CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD+YSWRKYGQKP
Sbjct: 241 KMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKP 300

Query: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGEHNHPRI-PSQSANT 336
           IKGSPHPRGYYKCSS+RGCPARKHVERC++E SMLIVTYEGEHNH RI  SQSA+T
Sbjct: 301 IKGSPHPRGYYKCSSVRGCPARKHVERCIDETSMLIVTYEGEHNHSRILSSQSAHT 330

BLAST of MS014810 vs. TAIR 10
Match: AT3G04670.2 (WRKY DNA-binding protein 39 )

HSP 1 Score: 203.0 bits (515), Expect = 3.8e-52
Identity = 150/312 (48.08%), Postives = 179/312 (57.37%), Query Frame = 0

Query: 1   MEEVEEANKAAVESCHRVLNLLSTPPQDQSKLRSLMAETGEAVFKFRKVVSLLNSSGLGH 60
           MEEVE AN++A+ESCH VLNLLS   Q  S  +SL  ETGE V KF++V SLL + GLGH
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLS---QRTSDPKSLTVETGEVVSKFKRVASLL-TRGLGH 60

Query: 61  ARVRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDL-------LQGKNLQMGPL 120
            + R    KF+  F            P  + LE+P  C  DL       L  + LQM P 
Sbjct: 61  GKFR-STNKFRSSF------------PQHIFLESPICCGNDLSGDYTQVLAPEPLQMVPA 120

Query: 121 CLSSNGKSSLQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGN---------NS 180
               N      Q    S    H +   +    L    P     Y L  N         NS
Sbjct: 121 SAVYNEMEPKHQLGHPSLMLSHKMCVDKS--FLELKPPPFRAPYQLIHNHQQIAYSRSNS 180

Query: 181 GINLNFD--SSSC-THTMSS-TRSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHK 240
           G+NL FD   SSC T ++S+ +RSF+SSLS+D SV + D   +FHL G  R SDQ  H +
Sbjct: 181 GVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYD-RNSFHLTGLSRGSDQ-QHTR 240

Query: 241 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 293
           + CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD+YSWRKYGQKP
Sbjct: 241 KMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKP 286

BLAST of MS014810 vs. TAIR 10
Match: AT4G24240.1 (WRKY DNA-binding protein 7 )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 125/323 (38.70%), Postives = 161/323 (49.85%), Query Frame = 0

Query: 4   VEEANKAAVESCHRVLNLLSTPPQDQSKLRS-LMAETGEAVFKFRKVVSLLNSSGLGHAR 63
           + EA  A +      L L+    Q   K ++ + A T  AV  F+KV+SLL  S  GHAR
Sbjct: 34  LREAASAGIHGVEEFLKLIGQSQQPTEKSQTEITAVTDVAVNSFKKVISLLGRSRTGHAR 93

Query: 64  VRKQLKKFQFQFQFQSQSQSSPFLPHTMLLENPPNCRTDLLQGKNLQMGPLCLSSNGKSS 123
            R+     Q  F+     Q+        + E  P   + L + K  Q         G S+
Sbjct: 94  FRRAPASTQTPFK-----QTPVVEEEVEVEEKKPETSSVLTKQKTEQY------HGGGSA 153

Query: 124 LQQAQSASAHYHHFLQQQQQTRVLLQNNPQAEMMYHLRGNNSGINLNFDSSSCTHTMSST 183
            +       H    L            N  +     +  N +   +NF  S     +S+T
Sbjct: 154 FRVYCPTPIHRRPPLSHNNNNNQNQTKNGSSSSSPPMLANGAPSTINFAPSP---PVSAT 213

Query: 184 RSFISSLSIDGSVANLDGSGAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSGRCHC 243
            SF+SS   D    ++  S  F      + S   S  K   S            S RCHC
Sbjct: 214 NSFMSSHRCDTDSTHM--SSGFEFTNPSQLS--GSRGKPPLSSASLKRRCNSSPSSRCHC 273

Query: 244 SKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPAR 303
           SKKRK RVKR I+VPA+S+K+ADIP D++SWRKYGQKPIKGSPHPRGYYKCSS+RGCPAR
Sbjct: 274 SKKRKSRVKRVIRVPAVSSKMADIPSDEFSWRKYGQKPIKGSPHPRGYYKCSSVRGCPAR 333

Query: 304 KHVERCLEEPSMLIVTYEGEHNH 326
           KHVER L++  MLIVTYEG+HNH
Sbjct: 334 KHVERALDDAMMLIVTYEGDHNH 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155836.11.2e-18599.11probable WRKY transcription factor 21 [Momordica charantia][more]
AWB50945.14.0e-13678.39WRKY transcription factor 21-like protein [Luffa aegyptiaca][more]
XP_038899952.11.1e-13378.37probable WRKY transcription factor 74 isoform X1 [Benincasa hispida][more]
KAA0059712.12.0e-13277.46putative WRKY transcription factor 21 [Cucumis melo var. makuwa] >TYK26150.1 put... [more]
XP_004146948.22.7e-13276.90probable WRKY transcription factor 21 [Cucumis sativus] >ADU52528.1 WRKY protein... [more]
Match NameE-valueIdentityDescription
O043363.2e-8853.39Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 ... [more]
Q93WU61.4e-7854.18Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 ... [more]
Q9SR079.1e-7552.81Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 ... [more]
Q32SG45.5e-7245.05Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1[more]
Q0JEE25.4e-4336.86WRKY transcription factor WRKY51 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Match NameE-valueIdentityDescription
A0A6J1DSX45.9e-18699.11probable WRKY transcription factor 21 OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A2S0USY91.9e-13678.39WRKY transcription factor 21-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A5D3DSD09.9e-13377.46Putative WRKY transcription factor 21 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
E7CEY51.3e-13276.90WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY51 PE=2 SV=1[more]
N1K0K33.8e-13276.62WRKY transcription factor 21 OS=Cucumis sativus OX=3659 GN=WRKY-21 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G30590.12.3e-8953.39WRKY DNA-binding protein 21 [more]
AT5G28650.19.6e-8054.18WRKY DNA-binding protein 74 [more]
AT3G04670.16.5e-7652.81WRKY DNA-binding protein 39 [more]
AT3G04670.23.8e-5248.08WRKY DNA-binding protein 39 [more]
AT4G24240.11.1e-4338.70WRKY DNA-binding protein 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 267..327
e-value: 2.0E-38
score: 143.6
IPR003657WRKY domainPFAMPF03106WRKYcoord: 269..326
e-value: 3.2E-26
score: 91.3
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 262..328
score: 30.067406
IPR018872Zn-cluster domainPFAMPF10533Plant_zn_clustcoord: 220..265
e-value: 3.5E-18
score: 65.4
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 252..328
e-value: 5.1E-31
score: 108.3
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 265..327
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..238
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..228
NoneNo IPR availablePANTHERPTHR31282:SF163WRKY DNA-BINDING PROTEIN 21-RELATEDcoord: 2..335
IPR044810WRKY transcription factor, plantPANTHERPTHR31282WRKY TRANSCRIPTION FACTOR 21-RELATEDcoord: 2..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014810.1MS014810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding