MS014728 (gene) Bitter gourd (TR) v1

Overview
NameMS014728
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold198: 115173 .. 116153 (+)
RNA-Seq ExpressionMS014728
SyntenyMS014728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCAAACTCCTGCTCTCCTCCCACACCCACCTTCTCACCCTTCCCCACAAACGCCCTTCATTGTGTCTTCACAATCACAATGGCGTCCTGCCCATCCGCTCAGTCCTCTCCGCTCCAGAGAAGCGTGGCAGAAAGAAGAGGCAGCCGCGGCACCCTAAGGATGACGCTTCCACTGCGCTTGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAGCCTCGACTCCGTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCCTTCCACGGCTTGGTTGTGTCTCATTCTCTCAACGGCGACACCGAGGGAGCGGTGAGCTGCTAATTTCAATTAAATATTGCTTTCGTTCCATTATAACTTTTGTTTTCAATTTACTTTCTGTTTCGGATATGTAGTTTTCTAAATTTTATTGGCTATGCTGAATGTAGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTCCATGAGACGTTTGTTGCATTAGTTCGGTTATTTGGTTCCAAGGGTCTTGCTTCCAGAGGCTTAGAAATCCTTTCAGCCATGGAGAAATTGAACTATGACATTCGCCAAGCTTGGCTCATTCTTATTGGTAATCTCTTGAACTTCCTGACGAGACTGCTTCCAACAGATACATTTTAATCATGTTTATATTTTGTCTTAGTTGCTTGAGTTATTTTGACTAGACGAACTCGTAAGGAATAAATACTTAGAAGACGCCAATAAAGCGTTTTTAAAGGGTGCCAAAGGGGGTCTCAGAGCCACTGACAAGATTTATGATCTCCTAATTGAGGAAGACTGTAAAGCTGGGGATCATTCAAATGCCTTAGAGATCTCATATGAGATGGAGGCTGCTGGGCGAATGGCAACAACCTTTCATTTCAATTGTCTCCTTAGTGTCCAGGTGTTGTATCTTACTATTGGA

mRNA sequence

ATGTCCAAACTCCTGCTCTCCTCCCACACCCACCTTCTCACCCTTCCCCACAAACGCCCTTCATTGTGTCTTCACAATCACAATGGCGTCCTGCCCATCCGCTCAGTCCTCTCCGCTCCAGAGAAGCGTGGCAGAAAGAAGAGGCAGCCGCGGCACCCTAAGGATGACGCTTCCACTGCGCTTGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAGCCTCGACTCCGTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCCTTCCACGGCTTGGTTGTGTCTCATTCTCTCAACGGCGACACCGAGGGAGCGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTCCATGAGACGTTTGTTGCATTAGTTCGGTTATTTGGTTCCAAGGGTCTTGCTTCCAGAGGCTTAGAAATCCTTTCAGCCATGGAGAAATTGAACTATGACATTCGCCAAGCTTGGCTCATTCTTATTGACGAACTCGTAAGGAATAAATACTTAGAAGACGCCAATAAAGCGTTTTTAAAGGGTGCCAAAGGGGGTCTCAGAGCCACTGACAAGATTTATGATCTCCTAATTGAGGAAGACTGTAAAGCTGGGGATCATTCAAATGCCTTAGAGATCTCATATGAGATGGAGGCTGCTGGGCGAATGGCAACAACCTTTCATTTCAATTGTCTCCTTAGTGTCCAGGTGTTGTATCTTACTATTGGA

Coding sequence (CDS)

ATGTCCAAACTCCTGCTCTCCTCCCACACCCACCTTCTCACCCTTCCCCACAAACGCCCTTCATTGTGTCTTCACAATCACAATGGCGTCCTGCCCATCCGCTCAGTCCTCTCCGCTCCAGAGAAGCGTGGCAGAAAGAAGAGGCAGCCGCGGCACCCTAAGGATGACGCTTCCACTGCGCTTGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAGCCTCGACTCCGTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCCTTCCACGGCTTGGTTGTGTCTCATTCTCTCAACGGCGACACCGAGGGAGCGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTCCATGAGACGTTTGTTGCATTAGTTCGGTTATTTGGTTCCAAGGGTCTTGCTTCCAGAGGCTTAGAAATCCTTTCAGCCATGGAGAAATTGAACTATGACATTCGCCAAGCTTGGCTCATTCTTATTGACGAACTCGTAAGGAATAAATACTTAGAAGACGCCAATAAAGCGTTTTTAAAGGGTGCCAAAGGGGGTCTCAGAGCCACTGACAAGATTTATGATCTCCTAATTGAGGAAGACTGTAAAGCTGGGGATCATTCAAATGCCTTAGAGATCTCATATGAGATGGAGGCTGCTGGGCGAATGGCAACAACCTTTCATTTCAATTGTCTCCTTAGTGTCCAGGTGTTGTATCTTACTATTGGA

Protein sequence

MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLSVQVLYLTIG
Homology
BLAST of MS014728 vs. NCBI nr
Match: XP_022151680.1 (uncharacterized protein LOC111019595 [Momordica charantia])

HSP 1 Score: 492.7 bits (1267), Expect = 2.0e-135
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA 60
           MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA
Sbjct: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA 60

Query: 61  LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA 120
           LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA
Sbjct: 61  LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA 120

Query: 121 MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL 180
           MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL
Sbjct: 121 MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL 180

Query: 181 VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT 240
           VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT
Sbjct: 181 VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT 240

Query: 241 FHFNCLLSVQ 251
           FHFNCLLSVQ
Sbjct: 241 FHFNCLLSVQ 250

BLAST of MS014728 vs. NCBI nr
Match: XP_038879291.1 (uncharacterized protein LOC120071230 [Benincasa hispida])

HSP 1 Score: 413.7 bits (1062), Expect = 1.2e-111
Identity = 222/255 (87.06%), Postives = 230/255 (90.20%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPR-----HPKD 60
           MSK LL SH HLLTLP+K  S  L NH GV+PIRSVLSAP+KRGRKKRQ R     H KD
Sbjct: 1   MSKFLL-SHAHLLTLPNKHHSFSL-NH-GVVPIRSVLSAPDKRGRKKRQARQQQQLHSKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
             STALEK LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH LNG
Sbjct: 61  HDSTALEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELSAGL PLHETFVALVRLFGSKGLA+RGLEIL+AMEKLNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSAGLCPLHETFVALVRLFGSKGLATRGLEILAAMEKLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. NCBI nr
Match: KAA0038380.1 (Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ97014.1 Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 412.1 bits (1058), Expect = 3.4e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. NCBI nr
Match: XP_008443747.1 (PREDICTED: uncharacterized protein LOC103487261 isoform X2 [Cucumis melo])

HSP 1 Score: 412.1 bits (1058), Expect = 3.4e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. NCBI nr
Match: XP_008443746.1 (PREDICTED: uncharacterized protein LOC103487261 isoform X1 [Cucumis melo])

HSP 1 Score: 412.1 bits (1058), Expect = 3.4e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. ExPASy Swiss-Prot
Match: Q9LER0 (Pentatricopeptide repeat-containing protein At5g14770, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g14770 PE=3 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 5.8e-05
Identity = 39/166 (23.49%), Postives = 72/166 (43.37%), Query Frame = 0

Query: 83  VGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVAL 142
           VG       M   G+ P   +F+ ++ S    GD+EG ++   +  S G++P   +   +
Sbjct: 557 VGADWAYKGMREKGIEPDIATFNIMMNSQRKQGDSEGILKLWDKMKSCGIKPSLMSCNIV 616

Query: 143 VRLFGSKGLASRGLEILSAMEKLN-YDIRQAWLILIDELVRNKYLEDANKAFLKGAKGGL 202
           V +    G     + IL+ M  +  +     + I +D   ++K  +   K        G+
Sbjct: 617 VGMLCENGKMEEAIHILNQMMLMEIHPNLTTYRIFLDTSSKHKRADAIFKTHETLLSYGI 676

Query: 203 RATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 248
           + + ++Y+ LI   CK G    A  +  +MEA G +  T  FN L+
Sbjct: 677 KLSRQVYNTLIATLCKLGMTKKAAMVMGDMEARGFIPDTVTFNSLM 722

BLAST of MS014728 vs. ExPASy Swiss-Prot
Match: Q0WPZ6 (Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX=3702 GN=At2g17140 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.7e-04
Identity = 40/149 (26.85%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 85  VSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVR 144
           VS +  DMV  G++P   +F+ L+ +   +   + A +        G +P   TF  LVR
Sbjct: 131 VSWLYKDMVLCGIAPQTYTFNLLIRALCDSSCVDAARELFDEMPEKGCKPNEFTFGILVR 190

Query: 145 LFGSKGLASRGLEILSAMEKLN-YDIRQAWLILIDELVRNKYLEDANKAFLKGAKGGLRA 204
            +   GL  +GLE+L+AME       +  +  ++    R    +D+ K   K  + GL  
Sbjct: 191 GYCKAGLTDKGLELLNAMESFGVLPNKVIYNTIVSSFCREGRNDDSEKMVEKMREEGLVP 250

Query: 205 TDKIYDLLIEEDCKAGDHSNALEISYEME 233
               ++  I   CK G   +A  I  +ME
Sbjct: 251 DIVTFNSRISALCKEGKVLDASRIFSDME 279

BLAST of MS014728 vs. ExPASy Swiss-Prot
Match: O64624 (Pentatricopeptide repeat-containing protein At2g18940, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g18940 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.7e-04
Identity = 34/122 (27.87%), Postives = 58/122 (47.54%), Query Frame = 0

Query: 129 SAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQ-AWLILIDELVRNKYLE 188
           S G  P   T+ AL+++FG  G+ +  L +L  ME+ +       +  L+   VR  + +
Sbjct: 309 SCGYEPGTVTYNALLQVFGKAGVYTEALSVLKEMEENSCPADSVTYNELVAAYVRAGFSK 368

Query: 189 DANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 248
           +A        K G+      Y  +I+   KAG    AL++ Y M+ AG +  T  +N +L
Sbjct: 369 EAAGVIEMMTKKGVMPNAITYTTVIDAYGKAGKEDEALKLFYSMKEAGCVPNTCTYNAVL 428

Query: 249 SV 250
           S+
Sbjct: 429 SL 430

BLAST of MS014728 vs. ExPASy Swiss-Prot
Match: O04504 (Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana OX=3702 GN=At1g09820 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.2e-04
Identity = 36/162 (22.22%), Postives = 72/162 (44.44%), Query Frame = 0

Query: 88  VIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFG 147
           V+ +MV   +SP   +F+ L+     + +  G+M+  +  L   ++P   ++ +L+    
Sbjct: 283 VLKEMVENDVSPNLTTFNILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLC 342

Query: 148 SKGLASRGLEILSAMEKLNYDIRQ-AWLILIDELVRNKYLEDANKAFLKGAKGGLRATDK 207
           + G  S  + +   M           +  LI+   +N  L++A   F      G   T +
Sbjct: 343 NGGKISEAISMRDKMVSAGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTR 402

Query: 208 IYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLS 249
           +Y++LI+  CK G   +   +  EME  G +     +NCL++
Sbjct: 403 MYNMLIDAYCKLGKIDDGFALKEEMEREGIVPDVGTYNCLIA 444

BLAST of MS014728 vs. ExPASy TrEMBL
Match: A0A6J1DBV3 (uncharacterized protein LOC111019595 OS=Momordica charantia OX=3673 GN=LOC111019595 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 9.6e-136
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA 60
           MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA
Sbjct: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHPKDDASTA 60

Query: 61  LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA 120
           LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA
Sbjct: 61  LEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGA 120

Query: 121 MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL 180
           MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL
Sbjct: 121 MQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDEL 180

Query: 181 VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT 240
           VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT
Sbjct: 181 VRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATT 240

Query: 241 FHFNCLLSVQ 251
           FHFNCLLSVQ
Sbjct: 241 FHFNCLLSVQ 250

BLAST of MS014728 vs. ExPASy TrEMBL
Match: A0A5A7T4U0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold506G00670 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.6e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. ExPASy TrEMBL
Match: A0A1S3B8T6 (uncharacterized protein LOC103487261 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487261 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.6e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. ExPASy TrEMBL
Match: A0A1S3B9H7 (uncharacterized protein LOC103487261 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487261 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.6e-111
Identity = 218/255 (85.49%), Postives = 231/255 (90.59%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVLPIRSVLSAPEKRGRKKRQPRHP-----KD 60
           MSKLLL SH HLLTLP+   S  L NH G+LPIRSVLSAP+KRGRKKRQ RH      KD
Sbjct: 1   MSKLLL-SHAHLLTLPYNHRSFSL-NH-GLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST+LE  LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNG
Sbjct: 61  DDSTSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNG 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           DTEGAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEIL+AME+LNYDIRQAWLI
Sbjct: 121 DTEGAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           L +ELVRNKYLEDANK FLKGAK GLRATDKIYDL+IEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LTEELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 252

BLAST of MS014728 vs. ExPASy TrEMBL
Match: A0A6J1EQ88 (uncharacterized protein LOC111436825 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436825 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.9e-107
Identity = 215/255 (84.31%), Postives = 225/255 (88.24%), Query Frame = 0

Query: 1   MSKLLLSSHTHLLTLPHKRPSLCLHNHNGVL-PIRSVLSAPEKRGRKKRQPR----HPKD 60
           MSK LL SH++LLTLPHK  S  L  HNGV  PIRSVLS  EKRGRKKRQ R      KD
Sbjct: 1   MSKFLL-SHSYLLTLPHKHHSFSL--HNGVFPPIRSVLST-EKRGRKKRQSRQQQLQQKD 60

Query: 61  DASTALEKGLRFTFMEELMDRARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNG 120
           D ST  EK LRFTFMEELMDRAR+ D +GVSDVIYDMVAAGLSPGPRSFHGLVVSH LN 
Sbjct: 61  DDSTVFEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNA 120

Query: 121 DTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLI 180
           D EGAMQSLR+ELS GLRPLHETFVALVRLFG+KGLA+RGLEIL+AMEKLNYDIRQAWLI
Sbjct: 121 DAEGAMQSLRKELSTGLRPLHETFVALVRLFGNKGLATRGLEILAAMEKLNYDIRQAWLI 180

Query: 181 LIDELVRNKYLEDANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240
           LI+ELV+NKYLEDANK FLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG
Sbjct: 181 LIEELVKNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAG 240

Query: 241 RMATTFHFNCLLSVQ 251
           RMATTFHFNCLLSVQ
Sbjct: 241 RMATTFHFNCLLSVQ 251

BLAST of MS014728 vs. TAIR 10
Match: AT3G04260.1 (plastid transcriptionally active 3 )

HSP 1 Score: 323.6 bits (828), Expect = 1.5e-88
Identity = 164/234 (70.09%), Postives = 196/234 (83.76%), Query Frame = 0

Query: 29  GVLPIRSVLSAPEKRGRKKRQPRH----PKDD--------ASTALEKGLRFTFMEELMDR 88
           G+  IR  +SAPEK+ R++R+ +       DD        A +ALE+ LR TFM+ELM+R
Sbjct: 24  GISSIRCSISAPEKKPRRRRKQKRGDGAENDDSLSFGSGEAVSALERSLRLTFMDELMER 83

Query: 89  ARSLDSVGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLH 148
           AR+ D+ GVS+VIYDM+AAGLSPGPRSFHGLVV+H+LNGD +GAM SLR+EL AG RPL 
Sbjct: 84  ARNRDTSGVSEVIYDMIAAGLSPGPRSFHGLVVAHALNGDEQGAMHSLRKELGAGQRPLP 143

Query: 149 ETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQAWLILIDELVRNKYLEDANKAFLKG 208
           ET +ALVRL GSKG A+RGLEIL+AMEKL YDIRQAWLIL++EL+R  +LEDANK FLKG
Sbjct: 144 ETMIALVRLSGSKGNATRGLEILAAMEKLKYDIRQAWLILVEELMRINHLEDANKVFLKG 203

Query: 209 AKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLSVQ 251
           A+GG+RATD++YDL+IEEDCKAGDHSNAL+ISYEMEAAGRMATTFHFNCLLSVQ
Sbjct: 204 ARGGMRATDQLYDLMIEEDCKAGDHSNALDISYEMEAAGRMATTFHFNCLLSVQ 257

BLAST of MS014728 vs. TAIR 10
Match: AT5G14770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 4.1e-06
Identity = 39/166 (23.49%), Postives = 72/166 (43.37%), Query Frame = 0

Query: 83  VGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVAL 142
           VG       M   G+ P   +F+ ++ S    GD+EG ++   +  S G++P   +   +
Sbjct: 555 VGADWAYKGMREKGIEPDIATFNIMMNSQRKQGDSEGILKLWDKMKSCGIKPSLMSCNIV 614

Query: 143 VRLFGSKGLASRGLEILSAMEKLN-YDIRQAWLILIDELVRNKYLEDANKAFLKGAKGGL 202
           V +    G     + IL+ M  +  +     + I +D   ++K  +   K        G+
Sbjct: 615 VGMLCENGKMEEAIHILNQMMLMEIHPNLTTYRIFLDTSSKHKRADAIFKTHETLLSYGI 674

Query: 203 RATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 248
           + + ++Y+ LI   CK G    A  +  +MEA G +  T  FN L+
Sbjct: 675 KLSRQVYNTLIATLCKLGMTKKAAMVMGDMEARGFIPDTVTFNSLM 720

BLAST of MS014728 vs. TAIR 10
Match: AT2G17140.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-05
Identity = 40/149 (26.85%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 85  VSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVR 144
           VS +  DMV  G++P   +F+ L+ +   +   + A +        G +P   TF  LVR
Sbjct: 131 VSWLYKDMVLCGIAPQTYTFNLLIRALCDSSCVDAARELFDEMPEKGCKPNEFTFGILVR 190

Query: 145 LFGSKGLASRGLEILSAMEKLN-YDIRQAWLILIDELVRNKYLEDANKAFLKGAKGGLRA 204
            +   GL  +GLE+L+AME       +  +  ++    R    +D+ K   K  + GL  
Sbjct: 191 GYCKAGLTDKGLELLNAMESFGVLPNKVIYNTIVSSFCREGRNDDSEKMVEKMREEGLVP 250

Query: 205 TDKIYDLLIEEDCKAGDHSNALEISYEME 233
               ++  I   CK G   +A  I  +ME
Sbjct: 251 DIVTFNSRISALCKEGKVLDASRIFSDME 279

BLAST of MS014728 vs. TAIR 10
Match: AT2G18940.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-05
Identity = 34/122 (27.87%), Postives = 58/122 (47.54%), Query Frame = 0

Query: 129 SAGLRPLHETFVALVRLFGSKGLASRGLEILSAMEKLNYDIRQ-AWLILIDELVRNKYLE 188
           S G  P   T+ AL+++FG  G+ +  L +L  ME+ +       +  L+   VR  + +
Sbjct: 309 SCGYEPGTVTYNALLQVFGKAGVYTEALSVLKEMEENSCPADSVTYNELVAAYVRAGFSK 368

Query: 189 DANKAFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 248
           +A        K G+      Y  +I+   KAG    AL++ Y M+ AG +  T  +N +L
Sbjct: 369 EAAGVIEMMTKKGVMPNAITYTTVIDAYGKAGKEDEALKLFYSMKEAGCVPNTCTYNAVL 428

Query: 249 SV 250
           S+
Sbjct: 429 SL 430

BLAST of MS014728 vs. TAIR 10
Match: AT1G09820.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.6e-05
Identity = 36/162 (22.22%), Postives = 72/162 (44.44%), Query Frame = 0

Query: 88  VIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFG 147
           V+ +MV   +SP   +F+ L+     + +  G+M+  +  L   ++P   ++ +L+    
Sbjct: 283 VLKEMVENDVSPNLTTFNILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLC 342

Query: 148 SKGLASRGLEILSAMEKLNYDIRQ-AWLILIDELVRNKYLEDANKAFLKGAKGGLRATDK 207
           + G  S  + +   M           +  LI+   +N  L++A   F      G   T +
Sbjct: 343 NGGKISEAISMRDKMVSAGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTR 402

Query: 208 IYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLS 249
           +Y++LI+  CK G   +   +  EME  G +     +NCL++
Sbjct: 403 MYNMLIDAYCKLGKIDDGFALKEEMEREGIVPDVGTYNCLIA 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151680.12.0e-135100.00uncharacterized protein LOC111019595 [Momordica charantia][more]
XP_038879291.11.2e-11187.06uncharacterized protein LOC120071230 [Benincasa hispida][more]
KAA0038380.13.4e-11185.49Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ97014... [more]
XP_008443747.13.4e-11185.49PREDICTED: uncharacterized protein LOC103487261 isoform X2 [Cucumis melo][more]
XP_008443746.13.4e-11185.49PREDICTED: uncharacterized protein LOC103487261 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LER05.8e-0523.49Pentatricopeptide repeat-containing protein At5g14770, mitochondrial OS=Arabidop... [more]
Q0WPZ61.7e-0426.85Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX... [more]
O646241.7e-0427.87Pentatricopeptide repeat-containing protein At2g18940, chloroplastic OS=Arabidop... [more]
O045042.2e-0422.22Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1DBV39.6e-136100.00uncharacterized protein LOC111019595 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A5A7T4U01.6e-11185.49Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B8T61.6e-11185.49uncharacterized protein LOC103487261 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3B9H71.6e-11185.49uncharacterized protein LOC103487261 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1EQ881.9e-10784.31uncharacterized protein LOC111436825 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT3G04260.11.5e-8870.09plastid transcriptionally active 3 [more]
AT5G14770.14.1e-0623.49Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G17140.11.2e-0526.85Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G18940.11.2e-0527.87Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G09820.11.6e-0522.22Pentatricopeptide repeat (PPR-like) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 70..211
e-value: 9.3E-10
score: 40.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..58
NoneNo IPR availablePANTHERPTHR31407FAMILY NOT NAMEDcoord: 27..250
NoneNo IPR availablePANTHERPTHR31407:SF5PLASTID TRANSCRIPTIONALLY ACTIVE 3coord: 27..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014728.1MS014728.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding