MS014686 (gene) Bitter gourd (TR) v1

BLAST of MS014686 vs. NCBI nr
Match: XP_022145285.1 (beta-amyrin 11-oxidase-like [Momordica charantia])

HSP 1 Score: 750.7 bits (1937), Expect = 8.7e-213
Identity = 362/418 (86.60%), Postives = 391/418 (93.54%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           MEFKNWLVL+LAAFVFVFGVLKRLNGWYYGA+L  IWAELPPGDM+WPL GSTLSFVKDV
Sbjct: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMSWPLLGSTLSFVKDV 60

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
           SNGRPQNF+ TFFNRYGKVDMFKTH+LGRASIIVCTPELCR+VLGDEEKFVPSLP+NIKL
Sbjct: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           L+G+KSL+Q SKAEHRRLRRL TAPISGH AL+MY+ HIEETVV GLEEW SMQRPIELL
Sbjct: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHTALEMYICHIEETVVGGLEEWESMQRPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           TEIKKLTFKVIWNIFMGSTT D SI EMEALF+DV+ GFMSLPINFPGFSFYKSFKARR 
Sbjct: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFHDVSLGFMSLPINFPGFSFYKSFKARRH 240

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           LSKILQ IINKKR VK+SKGE+WE KDM+DLLM+LKDEDGDEELDDETIID  FGKLFAG
Sbjct: 241 LSKILQSIINKKRSVKKSKGETWEAKDMMDLLMELKDEDGDEELDDETIIDFIFGKLFAG 300

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLTNHPHIFE+AKEEQE+IIRRRPST+KGI+ SEVKQM+ LS V+DE
Sbjct: 301 HETSAYTAMWAVLFLTNHPHIFEKAKEEQEKIIRRRPSTRKGIVPSEVKQMKSLSHVIDE 360

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           TFRVGGI ++LFREAT +VKINGKVIPKGW+V+ W GENYMDPKLFPSPQEFNPSRWD
Sbjct: 361 TFRVGGIIFLLFREATTNVKINGKVIPKGWKVITWFGENYMDPKLFPSPQEFNPSRWD 418

BLAST of MS014686 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-183
Identity = 311/418 (74.40%), Postives = 368/418 (88.04%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           ME KNWLVL++AAFVFV G+LKR+NGWYY AKLGK W +LPPG M WPL GST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
            +G+P NF+  F+ RYGK DM+KTHILGRA+IIVC+ ELCR+VLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFMANFYIRYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           LTGRKSLVQV KAEH+RLRRLT APISGHAAL+MY++HIE TV+SGLEEWASM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           +EIKKLTFK+IWNIFMGS++++SSI EME LF +VA GF+SLPINFPGF+ +K+ KAR++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  ILQ IIN+KRLV+E +G SWE KDM+DLLM++KDEDG EELDDETI DL FGKLFAG
Sbjct: 244 LLDILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDG-EELDDETIRDLIFGKLFAG 303

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLT HPHIF+ AKEEQ+ II +RP+TQKGIILSEVKQM++LSQ++DE
Sbjct: 304 HETSAYTAMWAVLFLTKHPHIFQRAKEEQDRIITQRPTTQKGIILSEVKQMKFLSQIIDE 363

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           T RVGG+T MLFREA VDV+INGK+IPKGW+V+ W GE YMD KLFPSPQEFNPSRWD
Sbjct: 364 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of MS014686 vs. NCBI nr
Match: XP_022923278.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita moschata] >KAG6577626.1 Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 652.1 bits (1681), Expect = 4.2e-183
Identity = 311/418 (74.40%), Postives = 366/418 (87.56%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           M  KNWLVL++AAFVFV G+LKR+NGWYY AKLGK W+ LPPGDM WPL GST+S++K  
Sbjct: 4   MGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSF 63

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
            +G+P NFI  F+ RYGK DM+KTHILGRA+IIVC+ ELCR+VLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           LTGRKSLVQV KAEH+RLRRLT APISGHAAL+MY++HIE TV+SGLEEWASM+ PIE+L
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           +EIKKLTFK+IWNIFMGS++++SSI EME LF +VA GF+SLPINFPGF+ +K+ KAR++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  ILQ IIN+KRLV+E +G SWE KDM+DLLM++KDEDG EELDDETI DL FGKLFAG
Sbjct: 244 LLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDG-EELDDETIRDLIFGKLFAG 303

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLT HPHIF+ AKEEQE I+ +RP+TQKGIILSEVKQM++L QV+DE
Sbjct: 304 HETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDE 363

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           T RVGG+T MLFREA VDV+INGK+IPKGW+V+ W GE YMD KLFPSPQEFNPSRWD
Sbjct: 364 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of MS014686 vs. NCBI nr
Match: XP_023007796.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 651.0 bits (1678), Expect = 9.4e-183
Identity = 309/418 (73.92%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           ME KNWLVL++AAFVFV G+LKRLNGWYY AKLGK W +LPPGDM WPL GST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
            +G+P NFI  F+ RYGK DM+KTH+LGRA+IIVC+ ELCR+VLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           LTGRKSLVQV KAEH+RLRRLT APISGHAAL+MY++HIE TV+SGLEEWASM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           +EIKKLTFK+IWNIFMGS++++SSI EME LF +VA GF+SLPINFPGF+ +K+ KAR++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  ILQ II++KRLV++ +G SWE KDM+DLLM++KDEDG EELDDETI DL FGKLFAG
Sbjct: 244 LLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDG-EELDDETIRDLIFGKLFAG 303

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLT HPHIF+ AKEEQE I+ +RP+TQKGIILSEVK+M++LSQ++DE
Sbjct: 304 HETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDE 363

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           T RVGG+T MLFREA VDV+INGK+IPKGW+V+ W GE YMD KLFPSP+EFNPSRWD
Sbjct: 364 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

BLAST of MS014686 vs. NCBI nr
Match: XP_022145397.1 (beta-amyrin 11-oxidase-like, partial [Momordica charantia])

HSP 1 Score: 639.4 bits (1648), Expect = 2.8e-179
Identity = 303/413 (73.37%), Postives = 366/413 (88.62%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           WL+L+L  FVFVFGVLKRLNGWYY  KLGKIWA+LPPGD++WPLFGSTLSF+K  + G P
Sbjct: 1   WLLLILGVFVFVFGVLKRLNGWYYAVKLGKIWAKLPPGDLDWPLFGSTLSFIKCFTIGSP 60

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
           +NFIRTF  RYGKVDM+KT+I GRA+I+VCTP++ R+VL DE KFVPS P  +K L GRK
Sbjct: 61  ENFIRTFSTRYGKVDMYKTNIFGRATILVCTPKISRQVLTDETKFVPSFPTTMKTLFGRK 120

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           SL++VSKAEHRRLRRLTTAP+SGH AL+MY++HIEETV+ GLEEWASMQRP+ELLTEIK+
Sbjct: 121 SLMRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKE 180

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTFKVIWNIFMGST++ S + EMEALFYD++ GF SLPINFPGF F+KS KAR++L +IL
Sbjct: 181 LTFKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEIL 240

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I++KKR VK++ G++WE KDM+DL+++++DEDG EELDDETIIDL FGKLFAGHETSA
Sbjct: 241 QSIVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDG-EELDDETIIDLIFGKLFAGHETSA 300

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
            TAMWAVLFLT+HPHIF++AKEEQEEIIRRRPSTQKG+ LSE K+M++LSQV++ET R+ 
Sbjct: 301 DTAMWAVLFLTDHPHIFQKAKEEQEEIIRRRPSTQKGVNLSEFKEMKFLSQVINETLRLT 360

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
            +T++LFREA VDV+INGK+IPKGW+V+ WL E YMD KL PSPQEFNPSRWD
Sbjct: 361 CMTFVLFREAIVDVEINGKIIPKGWKVLPWLREFYMDEKLHPSPQEFNPSRWD 412

BLAST of MS014686 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 5.4e-117
Identity = 207/427 (48.48%), Postives = 296/427 (69.32%), Query Frame = 0

Query: 10  VLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRPQNFI 69
           +L  ++F    ++ LNGWYY  KL +    LPPGDM WPL G  LSF+KD S+G P +FI
Sbjct: 13  LLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFI 72

Query: 70  RTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRKSLVQ 129
                +YG+  ++KTH+ G  SIIVC P++CR VL D+  F    P +IK L   + ++ 
Sbjct: 73  NNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMID 132

Query: 130 VSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKKLTFK 189
           VS AEHR  RRL T+PI GH AL MY+  +EE V++ LEE +SM+ P+ELL E+KK++FK
Sbjct: 133 VSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFK 192

Query: 190 VIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKILQLII 249
            I ++FMGS+  D  I+++ + F D+ +G  S+PIN PGF+F+K+ +AR++L+KI+Q ++
Sbjct: 193 AIVHVFMGSSNQD-IIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVV 252

Query: 250 NKKRL-VKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSAYTA 309
           +++RL ++    E  + KD++D+L+++KDE+G  +L+DE I DL  G LFAGHE++A + 
Sbjct: 253 DERRLMIENGPQEGSQRKDLIDILLEVKDENG-RKLEDEDISDLLIGLLFAGHESTATSL 312

Query: 310 MWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVGGIT 369
           MW++ +LT HPHI ++AKEEQEEI R R S+QK + L E+KQM YLSQV+DET R   I 
Sbjct: 313 MWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIA 372

Query: 370 YMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWDVRDLRKLVAE 429
           +  FREAT DV ING +IPKGWRV+ W    +MD + +P+P+EFNPSRWD  + +     
Sbjct: 373 FATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAK----A 432

Query: 430 GNQLPIG 436
           G  LP G
Sbjct: 433 GTFLPFG 433

BLAST of MS014686 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.0e-95
Identity = 175/413 (42.37%), Postives = 266/413 (64.41%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           W  L++     +  +++++N W Y + LG+    LPPGD+ WP  G+ LSF++      P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
            +F RT   RYG   ++K H+ G  SIIV T + CR VL D++ F P  P +   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           S V +S  EH+RLRRLT AP++GH AL  Y+ +IEE V++ L++W  M    E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTF++I  IF+ S + ++ ++ +E  +  +  G  ++ +N PGF+++++ KAR+ L    
Sbjct: 191 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 250

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I+ ++R  ++    S   KDM+D L+++KDEDG + LDDE IID+    L AGHE+S 
Sbjct: 251 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSG 310

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
           +T MWA +FL  HP + + AK EQE I++ RP  QKG+ L E ++M +LSQVVDET RV 
Sbjct: 311 HTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVI 370

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
             +   FREA  DV++NG +IPKGW+V+ W  + ++DP++FP P++F+P+RWD
Sbjct: 371 TFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWD 419

BLAST of MS014686 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 346.3 bits (887), Expect = 6.5e-94
Identity = 176/413 (42.62%), Postives = 269/413 (65.13%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           W  L++     +  VLKR+N W Y +KLG+    LPPGD+ WP+ G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
           ++FI+++  RYG+  ++K H+ G   ++V TPE CR VL D++ F    P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           S V +S  EH+RLRRLT+AP++G  AL +Y+  IEETV + LE+W+ M   IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTFKVI  IF+ S + +  ++ +E  + ++  G  ++ IN PGF+++++ KAR++L    
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I+  +R  ++    S   KDM+D L+D+KDE+G   LDDE IIDL    L AGHE+S 
Sbjct: 250 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 309

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
           +  MWA + +  HP I ++AKEEQE I+++R   QK + L E ++M YLSQV+DET RV 
Sbjct: 310 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 369

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
             +   FREA  DV+++G +IPKGW+V+ W    ++DP+++P P++F+PSRW+
Sbjct: 370 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of MS014686 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.5e-90
Identity = 172/411 (41.85%), Postives = 263/411 (63.99%), Query Frame = 0

Query: 10  VLAAFVFVFGVLKRLNGWYYGAKLG-KIWAELPPGDMNWPLFGSTLSFVKDVSNGRPQNF 69
           V+A  V V  V++R + W   A LG +  + LPPG+M WP+ GS  +F++   +G P  F
Sbjct: 24  VVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAF 83

Query: 70  IRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRKSLV 129
           I +F  R+G+  +++T +    +I+  TPE C++VL D+E FV   P     L G KS V
Sbjct: 84  IASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFV 143

Query: 130 QVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQR-PIELLTEIKKLT 189
            +S  +HRR+R+LT API+G  AL  Y+S I++TVV+ L  W+S +   +E LTE++++T
Sbjct: 144 NMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMT 203

Query: 190 FKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKILQL 249
           FK+I  IFM S   D+++E +E  + D+  G  ++ IN PGF++Y++ +ARR+L  +LQ 
Sbjct: 204 FKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQG 263

Query: 250 IINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSAYT 309
           +++ +R       +     DM+D L++ +DE G    DDE I+D+    L AGHE+S + 
Sbjct: 264 VLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYLNAGHESSGHI 323

Query: 310 AMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVGGI 369
            MWA +FL  +P IF  AK EQEEI+R  P+TQ G+ L + K+M +LSQVVDET R   I
Sbjct: 324 TMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNI 383

Query: 370 TYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           +++ FR+AT D+ +NG +IPKGW+V  W    +MD +++P P+ FNPSRW+
Sbjct: 384 SFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 432

BLAST of MS014686 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.1e-88
Identity = 166/407 (40.79%), Postives = 258/407 (63.39%), Query Frame = 0

Query: 13  AFVFVFGVLKRLNGWYYGAKLGKI-WAELPPGDMNWPLFGSTLSFVKDVSNGRPQNFIRT 72
           A V V    +R +GWY  A LG    A LPPG+M WPL G   +F++   +G+P  FI +
Sbjct: 44  ALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIAS 103

Query: 73  FFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRKSLVQVS 132
           F  R+G+  ++++ +    +++V T E C++VL D++ FV   P     L G +S V + 
Sbjct: 104 FVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMP 163

Query: 133 KAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKKLTFKVI 192
             EHRR+R+LT API+G  AL  Y+  I+ TV S L  WA     +E LTE++++TFK+I
Sbjct: 164 YDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKII 223

Query: 193 WNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKILQLIINK 252
             IF+G     ++   +E  + ++  G  ++ IN PGF++  + +ARR+L  +LQ ++++
Sbjct: 224 VQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDE 283

Query: 253 KRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSAYTAMWA 312
           +R  + +KG S  G DM+D L++ +DE G   LDD+ IID+    L AGHE+S +  MWA
Sbjct: 284 RRAAR-AKGVSGGGVDMMDRLIEAQDERG-RHLDDDEIIDVLVMYLNAGHESSGHITMWA 343

Query: 313 VLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVGGITYML 372
            +FL  +P +F  AK EQE I+R  PS+Q+G+ L + ++M YLSQV+DET R+  I+++ 
Sbjct: 344 TVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVS 403

Query: 373 FREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           FR+AT DV +NG +IPKGW+V  W    +MDP+++P P +F+PSRW+
Sbjct: 404 FRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWE 447

BLAST of MS014686 vs. ExPASy TrEMBL
Match: A0A6J1CU16 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014773 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 4.2e-213
Identity = 362/418 (86.60%), Postives = 391/418 (93.54%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           MEFKNWLVL+LAAFVFVFGVLKRLNGWYYGA+L  IWAELPPGDM+WPL GSTLSFVKDV
Sbjct: 1   MEFKNWLVLILAAFVFVFGVLKRLNGWYYGARLRNIWAELPPGDMSWPLLGSTLSFVKDV 60

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
           SNGRPQNF+ TFFNRYGKVDMFKTH+LGRASIIVCTPELCR+VLGDEEKFVPSLP+NIKL
Sbjct: 61  SNGRPQNFVHTFFNRYGKVDMFKTHVLGRASIIVCTPELCRQVLGDEEKFVPSLPVNIKL 120

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           L+G+KSL+Q SKAEHRRLRRL TAPISGH AL+MY+ HIEETVV GLEEW SMQRPIELL
Sbjct: 121 LSGKKSLIQFSKAEHRRLRRLMTAPISGHTALEMYICHIEETVVGGLEEWESMQRPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           TEIKKLTFKVIWNIFMGSTT D SI EMEALF+DV+ GFMSLPINFPGFSFYKSFKARR 
Sbjct: 181 TEIKKLTFKVIWNIFMGSTTTDYSIGEMEALFHDVSLGFMSLPINFPGFSFYKSFKARRH 240

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           LSKILQ IINKKR VK+SKGE+WE KDM+DLLM+LKDEDGDEELDDETIID  FGKLFAG
Sbjct: 241 LSKILQSIINKKRSVKKSKGETWEAKDMMDLLMELKDEDGDEELDDETIIDFIFGKLFAG 300

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLTNHPHIFE+AKEEQE+IIRRRPST+KGI+ SEVKQM+ LS V+DE
Sbjct: 301 HETSAYTAMWAVLFLTNHPHIFEKAKEEQEKIIRRRPSTRKGIVPSEVKQMKSLSHVIDE 360

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           TFRVGGI ++LFREAT +VKINGKVIPKGW+V+ W GENYMDPKLFPSPQEFNPSRWD
Sbjct: 361 TFRVGGIIFLLFREATTNVKINGKVIPKGWKVITWFGENYMDPKLFPSPQEFNPSRWD 418

BLAST of MS014686 vs. ExPASy TrEMBL
Match: A0A6J1E938 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431020 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 2.0e-183
Identity = 311/418 (74.40%), Postives = 366/418 (87.56%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           M  KNWLVL++AAFVFV G+LKR+NGWYY AKLGK W+ LPPGDM WPL GST+S++K  
Sbjct: 4   MGVKNWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWSMLPPGDMGWPLLGSTISYIKSF 63

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
            +G+P NFI  F+ RYGK DM+KTHILGRA+IIVC+ ELCR+VLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKGDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           LTGRKSLVQV KAEH+RLRRLT APISGHAAL+MY++HIE TV+SGLEEWASM+ PIE+L
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIEVL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           +EIKKLTFK+IWNIFMGS++++SSI EME LF +VA GF+SLPINFPGF+ +K+ KAR++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  ILQ IIN+KRLV+E +G SWE KDM+DLLM++KDEDG EELDDETI DL FGKLFAG
Sbjct: 244 LLNILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDG-EELDDETIRDLIFGKLFAG 303

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLT HPHIF+ AKEEQE I+ +RP+TQKGIILSEVKQM++L QV+DE
Sbjct: 304 HETSAYTAMWAVLFLTKHPHIFQRAKEEQERIVTQRPTTQKGIILSEVKQMKFLYQVIDE 363

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           T RVGG+T MLFREA VDV+INGK+IPKGW+V+ W GE YMD KLFPSPQEFNPSRWD
Sbjct: 364 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRWD 420

BLAST of MS014686 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 4.5e-183
Identity = 309/418 (73.92%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           ME KNWLVL++AAFVFV G+LKRLNGWYY AKLGK W +LPPGDM WPL GST+S++K+ 
Sbjct: 4   MEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNF 63

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
            +G+P NFI  F+ RYGK DM+KTH+LGRA+IIVC+ ELCR+VLGDE+ F PSLP NI +
Sbjct: 64  ISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINM 123

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           LTGRKSLVQV KAEH+RLRRLT APISGHAAL+MY++HIE TV+SGLEEWASM+ PIELL
Sbjct: 124 LTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELL 183

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           +EIKKLTFK+IWNIFMGS++++SSI EME LF +VA GF+SLPINFPGF+ +K+ KAR++
Sbjct: 184 SEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKK 243

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  ILQ II++KRLV++ +G SWE KDM+DLLM++KDEDG EELDDETI DL FGKLFAG
Sbjct: 244 LLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDG-EELDDETIRDLIFGKLFAG 303

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLT HPHIF+ AKEEQE I+ +RP+TQKGIILSEVK+M++LSQ++DE
Sbjct: 304 HETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDE 363

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
           T RVGG+T MLFREA VDV+INGK+IPKGW+V+ W GE YMD KLFPSP+EFNPSRWD
Sbjct: 364 TLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWD 420

BLAST of MS014686 vs. ExPASy TrEMBL
Match: A0A6J1CWG2 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014855 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.4e-179
Identity = 303/413 (73.37%), Postives = 366/413 (88.62%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           WL+L+L  FVFVFGVLKRLNGWYY  KLGKIWA+LPPGD++WPLFGSTLSF+K  + G P
Sbjct: 1   WLLLILGVFVFVFGVLKRLNGWYYAVKLGKIWAKLPPGDLDWPLFGSTLSFIKCFTIGSP 60

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
           +NFIRTF  RYGKVDM+KT+I GRA+I+VCTP++ R+VL DE KFVPS P  +K L GRK
Sbjct: 61  ENFIRTFSTRYGKVDMYKTNIFGRATILVCTPKISRQVLTDETKFVPSFPTTMKTLFGRK 120

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           SL++VSKAEHRRLRRLTTAP+SGH AL+MY++HIEETV+ GLEEWASMQRP+ELLTEIK+
Sbjct: 121 SLMRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKE 180

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTFKVIWNIFMGST++ S + EMEALFYD++ GF SLPINFPGF F+KS KAR++L +IL
Sbjct: 181 LTFKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEIL 240

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I++KKR VK++ G++WE KDM+DL+++++DEDG EELDDETIIDL FGKLFAGHETSA
Sbjct: 241 QSIVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDG-EELDDETIIDLIFGKLFAGHETSA 300

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
            TAMWAVLFLT+HPHIF++AKEEQEEIIRRRPSTQKG+ LSE K+M++LSQV++ET R+ 
Sbjct: 301 DTAMWAVLFLTDHPHIFQKAKEEQEEIIRRRPSTQKGVNLSEFKEMKFLSQVINETLRLT 360

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
            +T++LFREA VDV+INGK+IPKGW+V+ WL E YMD KL PSPQEFNPSRWD
Sbjct: 361 CMTFVLFREAIVDVEINGKIIPKGWKVLPWLREFYMDEKLHPSPQEFNPSRWD 412

BLAST of MS014686 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 3.0e-179
Identity = 300/419 (71.60%), Postives = 365/419 (87.11%), Query Frame = 0

Query: 1   MEFKNWLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDV 60
           ME K WLVL++ +   +FGV+KRLNGWYY AKLGK+W +LPPGDM WP+ GSTLSF+K+ 
Sbjct: 1   MELKEWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFLKNF 60

Query: 61  SNGRPQNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKL 120
           ++G+P+  +     RYGK DM+KTH+LGRASIIVCT E+CR+VL DEEKF PSLP +I L
Sbjct: 61  NSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITL 120

Query: 121 LTGRKSLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELL 180
           L+GRKSL+QV KAEHRRLRRLT APISGH+AL+MY++HIE+TV+SGLEEWASM++PIELL
Sbjct: 121 LSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELL 180

Query: 181 TEIKKLTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQ 240
           TEIKKLTFK+IWNIFMGS+++DSSI E+EALF  +A GF+SLPINFPGFSF+KS KAR++
Sbjct: 181 TEIKKLTFKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKE 240

Query: 241 LSKILQLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAG 300
           L  +LQ IIN+KR+ K+ +GE+WE KDM+DLL++LKDEDG EELDDETI DL FGKLFAG
Sbjct: 241 LLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDG-EELDDETIRDLIFGKLFAG 300

Query: 301 HETSAYTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDE 360
           HETSAYTAMWAVLFLTNHPHI +  KEEQE+I++RRP+TQKG+ LSEVKQM++L QV+DE
Sbjct: 301 HETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQVIDE 360

Query: 361 TFRVGGITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWDV 420
           T RVG IT+M+FREA VDV+INGK+IPKGW+V+ W GE YMD K FPSP EFNPSRWD+
Sbjct: 361 TLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRWDL 418

BLAST of MS014686 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-96
Identity = 175/413 (42.37%), Postives = 266/413 (64.41%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           W  L++     +  +++++N W Y + LG+    LPPGD+ WP  G+ LSF++      P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
            +F RT   RYG   ++K H+ G  SIIV T + CR VL D++ F P  P +   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           S V +S  EH+RLRRLT AP++GH AL  Y+ +IEE V++ L++W  M    E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTF++I  IF+ S + ++ ++ +E  +  +  G  ++ +N PGF+++++ KAR+ L    
Sbjct: 191 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 250

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I+ ++R  ++    S   KDM+D L+++KDEDG + LDDE IID+    L AGHE+S 
Sbjct: 251 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSG 310

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
           +T MWA +FL  HP + + AK EQE I++ RP  QKG+ L E ++M +LSQVVDET RV 
Sbjct: 311 HTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVI 370

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
             +   FREA  DV++NG +IPKGW+V+ W  + ++DP++FP P++F+P+RWD
Sbjct: 371 TFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWD 419

BLAST of MS014686 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 346.3 bits (887), Expect = 4.6e-95
Identity = 176/413 (42.62%), Postives = 269/413 (65.13%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           W  L++     +  VLKR+N W Y +KLG+    LPPGD+ WP+ G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
           ++FI+++  RYG+  ++K H+ G   ++V TPE CR VL D++ F    P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           S V +S  EH+RLRRLT+AP++G  AL +Y+  IEETV + LE+W+ M   IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTFKVI  IF+ S + +  ++ +E  + ++  G  ++ IN PGF+++++ KAR++L    
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I+  +R  ++    S   KDM+D L+D+KDE+G   LDDE IIDL    L AGHE+S 
Sbjct: 250 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 309

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
           +  MWA + +  HP I ++AKEEQE I+++R   QK + L E ++M YLSQV+DET RV 
Sbjct: 310 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 369

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
             +   FREA  DV+++G +IPKGW+V+ W    ++DP+++P P++F+PSRW+
Sbjct: 370 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of MS014686 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 346.3 bits (887), Expect = 4.6e-95
Identity = 176/413 (42.62%), Postives = 269/413 (65.13%), Query Frame = 0

Query: 6   WLVLVLAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRP 65
           W  L++     +  VLKR+N W Y +KLG+    LPPGD+ WP+ G+  SF++      P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRTFFNRYGKVDMFKTHILGRASIIVCTPELCREVLGDEEKFVPSLPMNIKLLTGRK 125
           ++FI+++  RYG+  ++K H+ G   ++V TPE CR VL D++ F    P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 SLVQVSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKK 185
           S V +S  EH+RLRRLT+AP++G  AL +Y+  IEETV + LE+W+ M   IE L+ ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKVIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKIL 245
           LTFKVI  IF+ S + +  ++ +E  + ++  G  ++ IN PGF+++++ KAR++L    
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 246 QLIINKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSA 305
           Q I+  +R  ++    S   KDM+D L+D+KDE+G   LDDE IIDL    L AGHE+S 
Sbjct: 250 QSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 309

Query: 306 YTAMWAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVG 365
           +  MWA + +  HP I ++AKEEQE I+++R   QK + L E ++M YLSQV+DET RV 
Sbjct: 310 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 369

Query: 366 GITYMLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWD 419
             +   FREA  DV+++G +IPKGW+V+ W    ++DP+++P P++F+PSRW+
Sbjct: 370 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

BLAST of MS014686 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 193.0 bits (489), Expect = 6.5e-49
Identity = 128/410 (31.22%), Postives = 209/410 (50.98%), Query Frame = 0

Query: 11  LAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRPQNFIR 70
           L     V  V+  L  W+   K  ++   LPPG M  P  G TL    +     P +F  
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRL--RLPPGSMGLPYIGETLRLYTE----NPNSFFA 81

Query: 71  TFFNRYGKVDMFKTHILGRASIIVCTPELCREVL-GDEEKFVPSLPMNIKLLTGRKSLVQ 130
           T  N+YG  D+FKTHILG   +++ +PE  R VL      F P+ P + + + G ++L  
Sbjct: 82  TRQNKYG--DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFF 141

Query: 131 VSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKKLTFK 190
                H  L+RL  +     +AL+  VSHIE  V+  L  W S Q+ I  L  +K+  F 
Sbjct: 142 HQGPYHSTLKRLVQSSFM-PSALRPTVSHIELLVLQTLSSWTS-QKSINTLEYMKRYAFD 201

Query: 191 VIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKILQLII 250
           V      G     ++I+ ++ L+  +  G+ S+P++ PG  F+KS KAR +LS+ L+ +I
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261

Query: 251 NKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSAYTAM 310
            K+R     +G       ++ +L+  KD+  +  L D  I D   G +FA  +T+A    
Sbjct: 262 EKRRENGREEG------GLLGVLLGAKDQKRN-GLSDSQIADNIIGVIFAATDTTASVLT 321

Query: 311 WAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVGGITY 370
           W + +L +HP++ +E   EQ  I ++     + I   + ++M   ++V+ ET R   +  
Sbjct: 322 WLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLS 381

Query: 371 MLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWDV 420
             FREA  DV+ +G +IPKGW+V+      +   + FP P++F+PSR++V
Sbjct: 382 FTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEV 414

BLAST of MS014686 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 193.0 bits (489), Expect = 6.5e-49
Identity = 128/410 (31.22%), Postives = 209/410 (50.98%), Query Frame = 0

Query: 11  LAAFVFVFGVLKRLNGWYYGAKLGKIWAELPPGDMNWPLFGSTLSFVKDVSNGRPQNFIR 70
           L     V  V+  L  W+   K  ++   LPPG M  P  G TL    +     P +F  
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRL--RLPPGSMGLPYIGETLRLYTE----NPNSFFA 81

Query: 71  TFFNRYGKVDMFKTHILGRASIIVCTPELCREVL-GDEEKFVPSLPMNIKLLTGRKSLVQ 130
           T  N+YG  D+FKTHILG   +++ +PE  R VL      F P+ P + + + G ++L  
Sbjct: 82  TRQNKYG--DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFF 141

Query: 131 VSKAEHRRLRRLTTAPISGHAALKMYVSHIEETVVSGLEEWASMQRPIELLTEIKKLTFK 190
                H  L+RL  +     +AL+  VSHIE  V+  L  W S Q+ I  L  +K+  F 
Sbjct: 142 HQGPYHSTLKRLVQSSFM-PSALRPTVSHIELLVLQTLSSWTS-QKSINTLEYMKRYAFD 201

Query: 191 VIWNIFMGSTTVDSSIEEMEALFYDVASGFMSLPINFPGFSFYKSFKARRQLSKILQLII 250
           V      G     ++I+ ++ L+  +  G+ S+P++ PG  F+KS KAR +LS+ L+ +I
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261

Query: 251 NKKRLVKESKGESWEGKDMVDLLMDLKDEDGDEELDDETIIDLTFGKLFAGHETSAYTAM 310
            K+R     +G       ++ +L+  KD+  +  L D  I D   G +FA  +T+A    
Sbjct: 262 EKRRENGREEG------GLLGVLLGAKDQKRN-GLSDSQIADNIIGVIFAATDTTASVLT 321

Query: 311 WAVLFLTNHPHIFEEAKEEQEEIIRRRPSTQKGIILSEVKQMRYLSQVVDETFRVGGITY 370
           W + +L +HP++ +E   EQ  I ++     + I   + ++M   ++V+ ET R   +  
Sbjct: 322 WLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLS 381

Query: 371 MLFREATVDVKINGKVIPKGWRVMAWLGENYMDPKLFPSPQEFNPSRWDV 420
             FREA  DV+ +G +IPKGW+V+      +   + FP P++F+PSR++V
Sbjct: 382 FTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEV 414