MS014645 (gene) Bitter gourd (TR) v1

BLAST of MS014645 vs. NCBI nr
Match: XP_023539522.1 (heavy metal-associated isoprenylated plant protein 35-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.2 bits (1071), Expect = 1.5e-112
Identity = 248/393 (63.10%), Postives = 284/393 (72.26%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTIIGWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIIGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH++ VV G  G+SH E+
Sbjct: 65  KTRKIATICSESEQ-ADPQASTQLPDN--PLPIGGEAEAPPPSHDMAVVDGLPGHSHAEV 124

Query: 121 TPGHVVVHETHSGPHSDPT----AITQPHGSHETGQ----------PGHVVHQHNSEPGH 180
            PG   VHE HSG   D T      TQP GSHE GQ           GHVVHQ + EPG 
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATVQATQPLGSHEVGQVHVTHNQPSEHGHVVHQSSLEPG- 184

Query: 181 VVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-HH 240
           VV H  PE G VV HQ PE+ HVV HQ PEHG V H Q+P +G V+H   P+H HVV HH
Sbjct: 185 VVHHLGPEPGQVVSHQ-PENSHVV-HQLPEHGHVGHQQSPMNGYVVHQHQPEHSHVVSHH 244

Query: 241 QAPEHGQVIHHQAPE--HGHVVHHRPSE--NGHVIHHQPTEHGHGHSTSSSYHHNI---- 300
           Q P+HG VIH Q PE  HGHV HH P+E  +GHV+ H  +E GHG ++S +YHHNI    
Sbjct: 245 QPPDHGHVIHQQPPEHSHGHVTHHHPAEHSHGHVVQHHQSEQGHGRTSSHNYHHNIPRTI 304

Query: 301 ---QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQPT--HYSPKIIDR 360
                  P+TV HSYNT++PSPYVTEYIRPLT+HKPSP AYE+ PQQ +  HYSP+ +DR
Sbjct: 305 HGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHKPSPYAYEYIPQQSSSNHYSPR-MDR 364

Query: 361 YSEEYYNTG-TSNNNGNITSIFSDENPNACTLM 364
           Y+++Y NTG +SNNNGNITSIFSDENPNAC LM
Sbjct: 365 YNDDYNNTGISSNNNGNITSIFSDENPNACNLM 390

BLAST of MS014645 vs. NCBI nr
Match: KAG6597556.1 (Heavy metal-associated isoprenylated plant protein 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415.6 bits (1067), Expect = 4.4e-112
Identity = 248/393 (63.10%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTI+GWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIVGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH EI
Sbjct: 65  KTRKIATICSESEQ-ADPLASTQLPDN--PLPSGGEAEAPPPSHDVAMVDGLPGHSHAEI 124

Query: 121 TPGHVVVHETHSGPHSDPT----AITQPHGSHETGQ----------PGHVVHQHNSEPGH 180
            PG   VHE HSG   D T      TQP GSHE GQ           GHVVHQ + EPG 
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATVQATQPLGSHEVGQVHVTHNQPSEHGHVVHQSSLEPG- 184

Query: 181 VVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-HH 240
           VV H  PE G VV HQ PE+ HVV HQ PEHG V H Q+P +G V+H   P+H HVV HH
Sbjct: 185 VVHHLGPEPGQVVSHQ-PENSHVV-HQLPEHGHVGHQQSPMNGYVVHQHQPEHSHVVSHH 244

Query: 241 QAPEHGQVIHHQAPE--HGHVVHHRPSE--NGHVIHHQPTEHGHGHSTSSSYHHNI---- 300
           Q P+HG VI+ Q PE  HGHV+HH P+E  +GHVI H  +E GHG ++S +YHHNI    
Sbjct: 245 QPPDHGHVINQQPPEHSHGHVIHHHPTEHTHGHVIQHHQSEQGHGRTSSHNYHHNIPRTI 304

Query: 301 ---QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQPT--HYSPKIIDR 360
                  P+TV HSYNT++PSPYVTEYIRPLT+HKPSP AYE+ PQQ +  HYSP+ +DR
Sbjct: 305 HGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHKPSPYAYEYIPQQSSSNHYSPR-MDR 364

Query: 361 YSEEYYNTG-TSNNNGNITSIFSDENPNACTLM 364
           Y+++Y NTG +SNNNGNITSIFSDENPNAC LM
Sbjct: 365 YNDDYNNTGISSNNNGNITSIFSDENPNACNLM 390

BLAST of MS014645 vs. NCBI nr
Match: XP_022939762.1 (heavy metal-associated isoprenylated plant protein 35-like [Cucurbita moschata])

HSP 1 Score: 415.6 bits (1067), Expect = 4.4e-112
Identity = 248/393 (63.10%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTI+GWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIVGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH EI
Sbjct: 65  KTRKIATICSESEQ-ADPLASTQLPDN--PLPSGGEAEAPPPSHDVAMVDGLPGHSHVEI 124

Query: 121 TPGHVVVHETHSGPHSDPT----AITQPHGSHETGQ----------PGHVVHQHNSEPGH 180
            PG   VHE HSG   D T      TQP GSHE GQ           GHVVHQ + EPG 
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATVQATQPLGSHEVGQVHVTHNQPSEHGHVVHQSSLEPG- 184

Query: 181 VVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-HH 240
           VV H  PE G VV HQ PE+ HVV HQ PEHG V H Q+P +G V+H   P+H HVV HH
Sbjct: 185 VVHHLGPEPGQVVSHQ-PENSHVV-HQLPEHGHVGHQQSPMNGYVVHQHQPEHSHVVSHH 244

Query: 241 QAPEHGQVIHHQAPE--HGHVVHHRPSE--NGHVIHHQPTEHGHGHSTSSSYHHNI---- 300
           Q P+HG VI+ Q PE  HGHV+HH P+E  +GHVI H  +E GHG ++S +YHHNI    
Sbjct: 245 QPPDHGHVINQQPPEHSHGHVIHHHPTEHTHGHVIQHHQSEQGHGRTSSHNYHHNIPRTI 304

Query: 301 ---QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQPT--HYSPKIIDR 360
                  P+TV HSYNT++PSPYVTEYIRPLT+HKPSP AYE+ PQQ +  HYSP+ +DR
Sbjct: 305 HGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHKPSPYAYEYIPQQSSSNHYSPR-MDR 364

Query: 361 YSEEYYNTG-TSNNNGNITSIFSDENPNACTLM 364
           Y+++Y NTG +SNNNGNITSIFSDENPNAC LM
Sbjct: 365 YNDDYNNTGISSNNNGNITSIFSDENPNACNLM 390

BLAST of MS014645 vs. NCBI nr
Match: KAG7029001.1 (Heavy metal-associated isoprenylated plant protein 28 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.2 bits (1066), Expect = 5.7e-112
Identity = 247/393 (62.85%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTI+GWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIVGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH EI
Sbjct: 65  KTRKIATICSESEQ-ADPLASTQLPDN--PLPSGGEAEAPPPSHDVAMVDGLPGHSHVEI 124

Query: 121 TPGHVVVHETHSGPHSDPT----AITQPHGSHETGQ----------PGHVVHQHNSEPGH 180
            PG   VHE HSG   D T      TQP GSHE GQ           GHVVHQ + EPG 
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATVQATQPLGSHEVGQVHVTHNQPSEHGHVVHQSSLEPG- 184

Query: 181 VVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-HH 240
           VV H  PE G VV HQ PE+ HVV HQ PEHG V H Q+P +G V+H   P+H HVV HH
Sbjct: 185 VVHHLGPEPGQVVSHQ-PENSHVV-HQLPEHGHVGHQQSPMNGYVVHQHQPEHSHVVSHH 244

Query: 241 QAPEHGQVIHHQAPE--HGHVVHHRPSE--NGHVIHHQPTEHGHGHSTSSSYHHNI---- 300
           Q P+HG VI+ Q PE  HGHV+HH P+E  +GHV+ H  +E GHG ++S +YHHNI    
Sbjct: 245 QPPDHGHVINQQPPEHSHGHVIHHHPTEHTHGHVVQHHQSEQGHGRTSSHNYHHNIPRTI 304

Query: 301 ---QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQPT--HYSPKIIDR 360
                  P+TV HSYNT++PSPYVTEYIRPLT+HKPSP AYE+ PQQ +  HYSP+ +DR
Sbjct: 305 HGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHKPSPYAYEYIPQQSSSNHYSPR-MDR 364

Query: 361 YSEEYYNTG-TSNNNGNITSIFSDENPNACTLM 364
           Y+++Y NTG +SNNNGNITSIFSDENPNAC LM
Sbjct: 365 YNDDYNNTGISSNNNGNITSIFSDENPNACNLM 390

BLAST of MS014645 vs. NCBI nr
Match: XP_022972279.1 (LIM domain-containing protein A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 402.9 bits (1034), Expect = 2.9e-108
Identity = 241/390 (61.79%), Postives = 275/390 (70.51%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTIIGWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIIGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH E+
Sbjct: 65  KTRKIATICSEYEQ-ADPQASTQLPDN--PLPSGGEAEAPTPSHDVAMVDGLPGHSHAEV 124

Query: 121 TPGHVVVHETHSGPHSDPT-----AITQPHGSHETG----------QPGHVVHQHNSEPG 180
            PG   VHE HSG   D T       TQP GSHE G          Q GHVVHQ + EPG
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATNQQATQPLGSHEVGQVHVTHNQPSQHGHVVHQSSLEPG 184

Query: 181 HVVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-H 240
             V H  PE GHVV HQ PE+ HV  HQ PEHG V H Q+P +G V+H   P+H HVV H
Sbjct: 185 D-VHHLGPESGHVVSHQ-PENSHVA-HQLPEHGHVGHQQSPINGYVVHQHQPEHSHVVSH 244

Query: 241 HQAPEHGQVIHHQAPEHGHVVHHRPSENGHVIHHQPTEHGHGHSTSSSYHHNI------- 300
           HQ P+HG VIH Q PEH H  H     + HV+ H  +E GHG ++S +YHHNI       
Sbjct: 245 HQPPDHGHVIHQQPPEHSH-SHPTEHSHDHVVQHHQSEQGHGRTSSHNYHHNIPRTIHGS 304

Query: 301 QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQ--PTHYSPKIIDRYSE 360
               P+TV HSYNT++PSPYVTEYIRPLT+H+PSP AYE+ PQQ   THYSP+ +DRY++
Sbjct: 305 TSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHRPSPYAYEYIPQQSSSTHYSPR-MDRYND 364

Query: 361 EYYNTGTS-NNNGNITSIFSDENPNACTLM 364
           +Y NTG S NNNGNITSIFSDENPNAC LM
Sbjct: 365 DYNNTGISNNNNGNITSIFSDENPNACNLM 386

BLAST of MS014645 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.5e-09
Identity = 30/61 (49.18%), Postives = 42/61 (68.85%), Query Frame = 0

Query: 8  EIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKIAT 67
          E+KVRMDC+GCV KIK +L  + G+  + I+  QQK+T+ G+AD  K++K  K T K A 
Sbjct: 35 ELKVRMDCDGCVLKIKNSLSSLKGVKTVEINKKQQKVTVSGYADASKVLKKAKATGKKAE 94

Query: 68 I 69
          I
Sbjct: 95 I 95

BLAST of MS014645 vs. ExPASy Swiss-Prot
Match: F4IC29 (Heavy metal-associated isoprenylated plant protein 28 OS=Arabidopsis thaliana OX=3702 GN=HIPP28 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.9e-08
Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 0

Query: 8   EIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKIAT 67
           E++V MDC GC  ++K AL  + G+  + ID  QQK+T+ G+AD  K++K ++KT + A 
Sbjct: 14  EMRVHMDCVGCESRVKNALQKMRGVDAVEIDMVQQKVTVTGYADQKKVLKKVRKTGRRAE 73

Query: 68  ICSDPEQPTDPHAAAQPPDHHPPQHHGGETAN 100
           +   P               + P H GG ++N
Sbjct: 74  LWQLP---------------YNPDHMGGSSSN 90

BLAST of MS014645 vs. ExPASy Swiss-Prot
Match: O81464 (Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX=3702 GN=HIPP24 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 23/53 (43.40%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 12 RMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRK 65
          R+DC GC +KIK  L G+ G+  + +D   QK+T+ G+ DP K+++A K T+K
Sbjct: 34 RIDCEGCERKIKHVLSGVKGVKSVDVDVKLQKVTVTGYIDPKKVLEAAKSTKK 86

BLAST of MS014645 vs. ExPASy Swiss-Prot
Match: B3H6D0 (Heavy metal-associated isoprenylated plant protein 45 OS=Arabidopsis thaliana OX=3702 GN=HIPP45 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.3e-07
Identity = 22/61 (36.07%), Postives = 43/61 (70.49%), Query Frame = 0

Query: 6  ITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKI 65
          I E+ V MDC GC +K+++A+  ++G+  + ID  +QK+T+ G+ D ++++K +K+T + 
Sbjct: 17 IVELLVDMDCKGCEKKVRRAISKLDGVDTVEIDVDRQKVTVTGYVDREEVLKMVKRTGRT 76

Query: 66 A 67
          A
Sbjct: 77 A 77

BLAST of MS014645 vs. ExPASy Swiss-Prot
Match: Q9LP41 (Heavy metal-associated isoprenylated plant protein 29 OS=Arabidopsis thaliana OX=3702 GN=HIPP29 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 5.2e-07
Identity = 26/68 (38.24%), Postives = 44/68 (64.71%), Query Frame = 0

Query: 9  IKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKK-TRKIAT 68
          ++V MDC GC  K++KAL  +NG++D+ ID  QQ++T+ G A+  K++K  +  T++   
Sbjct: 1  MEVPMDCPGCENKVRKALEKMNGVHDVQIDIKQQRVTVTGSAEQKKVLKVARNVTKRDIC 60

Query: 69 ICSDPEQP 76
          + S P  P
Sbjct: 61 LWSYPYHP 68

BLAST of MS014645 vs. ExPASy TrEMBL
Match: A0A6J1FHQ9 (heavy metal-associated isoprenylated plant protein 35-like OS=Cucurbita moschata OX=3662 GN=LOC111445545 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 2.1e-112
Identity = 248/393 (63.10%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTI+GWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIVGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH EI
Sbjct: 65  KTRKIATICSESEQ-ADPLASTQLPDN--PLPSGGEAEAPPPSHDVAMVDGLPGHSHVEI 124

Query: 121 TPGHVVVHETHSGPHSDPT----AITQPHGSHETGQ----------PGHVVHQHNSEPGH 180
            PG   VHE HSG   D T      TQP GSHE GQ           GHVVHQ + EPG 
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATVQATQPLGSHEVGQVHVTHNQPSEHGHVVHQSSLEPG- 184

Query: 181 VVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-HH 240
           VV H  PE G VV HQ PE+ HVV HQ PEHG V H Q+P +G V+H   P+H HVV HH
Sbjct: 185 VVHHLGPEPGQVVSHQ-PENSHVV-HQLPEHGHVGHQQSPMNGYVVHQHQPEHSHVVSHH 244

Query: 241 QAPEHGQVIHHQAPE--HGHVVHHRPSE--NGHVIHHQPTEHGHGHSTSSSYHHNI---- 300
           Q P+HG VI+ Q PE  HGHV+HH P+E  +GHVI H  +E GHG ++S +YHHNI    
Sbjct: 245 QPPDHGHVINQQPPEHSHGHVIHHHPTEHTHGHVIQHHQSEQGHGRTSSHNYHHNIPRTI 304

Query: 301 ---QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQPT--HYSPKIIDR 360
                  P+TV HSYNT++PSPYVTEYIRPLT+HKPSP AYE+ PQQ +  HYSP+ +DR
Sbjct: 305 HGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHKPSPYAYEYIPQQSSSNHYSPR-MDR 364

Query: 361 YSEEYYNTG-TSNNNGNITSIFSDENPNACTLM 364
           Y+++Y NTG +SNNNGNITSIFSDENPNAC LM
Sbjct: 365 YNDDYNNTGISSNNNGNITSIFSDENPNACNLM 390

BLAST of MS014645 vs. ExPASy TrEMBL
Match: A0A6J1IB14 (LIM domain-containing protein A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470859 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.4e-108
Identity = 241/390 (61.79%), Postives = 275/390 (70.51%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTIIGWADPD+IMKAIK
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIIGWADPDRIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGET-ANIPSHEVVVVGGSNGNSHTEI 120
           KTRKIATICS+ EQ  DP A+ Q PD+  P   GGE  A  PSH+V +V G  G+SH E+
Sbjct: 65  KTRKIATICSEYEQ-ADPQASTQLPDN--PLPSGGEAEAPTPSHDVAMVDGLPGHSHAEV 124

Query: 121 TPGHVVVHETHSGPHSDPT-----AITQPHGSHETG----------QPGHVVHQHNSEPG 180
            PG   VHE HSG   D T       TQP GSHE G          Q GHVVHQ + EPG
Sbjct: 125 APGQTTVHEAHSGQQFDLTNATNQQATQPLGSHEVGQVHVTHNQPSQHGHVVHQSSLEPG 184

Query: 181 HVVQHNVPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV-H 240
             V H  PE GHVV HQ PE+ HV  HQ PEHG V H Q+P +G V+H   P+H HVV H
Sbjct: 185 D-VHHLGPESGHVVSHQ-PENSHVA-HQLPEHGHVGHQQSPINGYVVHQHQPEHSHVVSH 244

Query: 241 HQAPEHGQVIHHQAPEHGHVVHHRPSENGHVIHHQPTEHGHGHSTSSSYHHNI------- 300
           HQ P+HG VIH Q PEH H  H     + HV+ H  +E GHG ++S +YHHNI       
Sbjct: 245 HQPPDHGHVIHQQPPEHSH-SHPTEHSHDHVVQHHQSEQGHGRTSSHNYHHNIPRTIHGS 304

Query: 301 QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQ--PTHYSPKIIDRYSE 360
               P+TV HSYNT++PSPYVTEYIRPLT+H+PSP AYE+ PQQ   THYSP+ +DRY++
Sbjct: 305 TSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHRPSPYAYEYIPQQSSSTHYSPR-MDRYND 364

Query: 361 EYYNTGTS-NNNGNITSIFSDENPNACTLM 364
           +Y NTG S NNNGNITSIFSDENPNAC LM
Sbjct: 365 DYNNTGISNNNNGNITSIFSDENPNACNLM 386

BLAST of MS014645 vs. ExPASy TrEMBL
Match: A0A6J1I9F1 (LIM domain-containing protein A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470859 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 4.7e-104
Identity = 241/418 (57.66%), Postives = 275/418 (65.79%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGIN----------------------------GI 60
           L+KPKI EIKVRMDCNGCVQKIKKALYGIN                            GI
Sbjct: 5   LEKPKILEIKVRMDCNGCVQKIKKALYGINGELNEPGFFVFFVFHMFVSCSSISWPVAGI 64

Query: 61  YDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKIATICSDPEQPTDPHAAAQPPDHHPPQH 120
           YD+YIDFPQQKLTIIGWADPD+IMKAIKKTRKIATICS+ EQ  DP A+ Q PD+  P  
Sbjct: 65  YDIYIDFPQQKLTIIGWADPDRIMKAIKKTRKIATICSEYEQ-ADPQASTQLPDN--PLP 124

Query: 121 HGGET-ANIPSHEVVVVGGSNGNSHTEITPGHVVVHETHSGPHSDPT-----AITQPHGS 180
            GGE  A  PSH+V +V G  G+SH E+ PG   VHE HSG   D T       TQP GS
Sbjct: 125 SGGEAEAPTPSHDVAMVDGLPGHSHAEVAPGQTTVHEAHSGQQFDLTNATNQQATQPLGS 184

Query: 181 HETG----------QPGHVVHQHNSEPGHVVQHNVPEHGHVVHHQAPEHGHVVHHQAPEH 240
           HE G          Q GHVVHQ + EPG  V H  PE GHVV HQ PE+ HV  HQ PEH
Sbjct: 185 HEVGQVHVTHNQPSQHGHVVHQSSLEPGD-VHHLGPESGHVVSHQ-PENSHVA-HQLPEH 244

Query: 241 GQVIHHQAPQHGQVIHHQAPQHGHVV-HHQAPEHGQVIHHQAPEHGHVVHHRPSENGHVI 300
           G V H Q+P +G V+H   P+H HVV HHQ P+HG VIH Q PEH H  H     + HV+
Sbjct: 245 GHVGHQQSPINGYVVHQHQPEHSHVVSHHQPPDHGHVIHQQPPEHSH-SHPTEHSHDHVV 304

Query: 301 HHQPTEHGHGHSTSSSYHHNI-------QGAAPVTVTHSYNTHKPSPYVTEYIRPLTSHK 360
            H  +E GHG ++S +YHHNI           P+TV HSYNT++PSPYVTEYIRPLT+H+
Sbjct: 305 QHHQSEQGHGRTSSHNYHHNIPRTIHGSTSTHPMTVIHSYNTYQPSPYVTEYIRPLTTHR 364

Query: 361 PSPNAYEHTPQQ--PTHYSPKIIDRYSEEYYNTGTS-NNNGNITSIFSDENPNACTLM 364
           PSP AYE+ PQQ   THYSP+ +DRY+++Y NTG S NNNGNITSIFSDENPNAC LM
Sbjct: 365 PSPYAYEYIPQQSSSTHYSPR-MDRYNDDYNNTGISNNNNGNITSIFSDENPNACNLM 414

BLAST of MS014645 vs. ExPASy TrEMBL
Match: A0A6J1C910 (heavy metal-associated isoprenylated plant protein 5-like OS=Momordica charantia OX=3673 GN=LOC111009317 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 175/208 (84.13%), Postives = 180/208 (86.54%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK
Sbjct: 5   LEKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGETANIPSHEVVVVGGSNGNSHTEIT 120
           KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGETANIPSHEVVVVGGSNGNSHTEIT
Sbjct: 65  KTRKIATICSDPEQPTDPHAAAQPPDHHPPQHHGGETANIPSHEVVVVGGSNGNSHTEIT 124

Query: 121 PGHVVVHETHSGPHSDPTAITQPHGSHETGQPGHVVHQHNSEPGHVVQHNVPEHGHVVHH 180
           PGHVVVHETHSG HSDPT +TQPHG HETGQ             HV  H  PEHGHV  H
Sbjct: 125 PGHVVVHETHSGSHSDPTVMTQPHGLHETGQV------------HVTHHQPPEHGHVTVH 184

Query: 181 QAPEHGHVVHHQAPEHGQVIHHQA-PQH 208
           QAPEHGHV +H APEHG V+HH + P H
Sbjct: 185 QAPEHGHVTYHHAPEHGHVVHHTSEPGH 200

BLAST of MS014645 vs. ExPASy TrEMBL
Match: A0A0A0L937 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165100 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 4.7e-88
Identity = 218/390 (55.90%), Postives = 258/390 (66.15%), Query Frame = 0

Query: 1   LQKPKITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIK 60
           L+KPKITEIKVRMDCNGCVQKIKKALYGINGIYD+YIDFPQQKLTIIGWA+P++IMKAIK
Sbjct: 5   LEKPKITEIKVRMDCNGCVQKIKKALYGINGIYDIYIDFPQQKLTIIGWAEPERIMKAIK 64

Query: 61  KTRKIATICSDPEQPTDPHAA--AQPPDHHPPQHHGGETANI---PSHEVVVVGGSNGNS 120
           KTRKIATICSD EQ TDP AA   QPPD   P   G E  N    PS   +V+GG   + 
Sbjct: 65  KTRKIATICSDSEQ-TDPQAAPTGQPPDSMAPA--GSEPPNFSSGPSPPDMVMGGGPTDM 124

Query: 121 HT--EITPGHVVVHE-------THSGPHSDPTAITQPHGSHETGQPGHVVHQHNSEPGHV 180
            T     PG +VV +           PH       Q H SH+    GH+VHQ+ SE GHV
Sbjct: 125 ATGGAPPPGQMVVQDGSFDPTNGQQPPHPGGYDAGQGHVSHQPSDNGHIVHQYPSESGHV 184

Query: 181 VQHN----VPEHGHVVHHQAPEHGHVVHHQAPEHGQVIHHQAPQHGQVIHHQAPQHGHVV 240
           V H      PE GHV+ HQ PE+ HVVHHQ PE   V  HQ  +H  ++ +Q P HGH V
Sbjct: 185 VHHQGSGPGPEPGHVISHQ-PEYSHVVHHQPPESFHV-GHQPSEHVHMV-NQPPDHGHTV 244

Query: 241 H-HQAPEHGQVI-HHQAPEH--GHVVHHRPSENGHVIHHQPTEHGHGHSTSSSYHHNIQG 300
           + HQ P+H Q + +HQ P +   HVVH          HHQP    + +++S +YHHNI G
Sbjct: 245 YSHQPPDHSQTVQYHQPPNYSQSHVVH----------HHQPE---YRYASSHNYHHNIPG 304

Query: 301 AA---PVTVTHSYNTHKPSPYVTEYIRPLTSHKPSPNAYEHTPQQP-THYSPKI-IDRYS 360
           A    P TV HSYNT+KPSPY+TEY+RPLT  +P   A E  PQQP THYSP++ +  Y+
Sbjct: 305 ATSMQPATVIHSYNTYKPSPYITEYVRPLTYSRPPVYASESIPQQPTTHYSPRLDLYNYN 364

Query: 361 EEYYNTGTSNNNGNITSIFSDENPNACTLM 364
           E+YYNTGT ++NGNITSIFSDENPNAC LM
Sbjct: 365 EDYYNTGTGSSNGNITSIFSDENPNACNLM 375

BLAST of MS014645 vs. TAIR 10
Match: AT4G39700.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.8e-10
Identity = 30/61 (49.18%), Postives = 42/61 (68.85%), Query Frame = 0

Query: 8  EIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKIAT 67
          E+KVRMDC+GCV KIK +L  + G+  + I+  QQK+T+ G+AD  K++K  K T K A 
Sbjct: 35 ELKVRMDCDGCVLKIKNSLSSLKGVKTVEINKKQQKVTVSGYADASKVLKKAKATGKKAE 94

Query: 68 I 69
          I
Sbjct: 95 I 95

BLAST of MS014645 vs. TAIR 10
Match: AT1G06330.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.6e-09
Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 0

Query: 8   EIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKIAT 67
           E++V MDC GC  ++K AL  + G+  + ID  QQK+T+ G+AD  K++K ++KT + A 
Sbjct: 14  EMRVHMDCVGCESRVKNALQKMRGVDAVEIDMVQQKVTVTGYADQKKVLKKVRKTGRRAE 73

Query: 68  ICSDPEQPTDPHAAAQPPDHHPPQHHGGETAN 100
           +   P               + P H GG ++N
Sbjct: 74  LWQLP---------------YNPDHMGGSSSN 90

BLAST of MS014645 vs. TAIR 10
Match: AT4G08570.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 9.6e-09
Identity = 23/53 (43.40%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 12 RMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRK 65
          R+DC GC +KIK  L G+ G+  + +D   QK+T+ G+ DP K+++A K T+K
Sbjct: 34 RIDCEGCERKIKHVLSGVKGVKSVDVDVKLQKVTVTGYIDPKKVLEAAKSTKK 86

BLAST of MS014645 vs. TAIR 10
Match: AT3G56891.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-08
Identity = 22/61 (36.07%), Postives = 43/61 (70.49%), Query Frame = 0

Query: 6  ITEIKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKKTRKI 65
          I E+ V MDC GC +K+++A+  ++G+  + ID  +QK+T+ G+ D ++++K +K+T + 
Sbjct: 17 IVELLVDMDCKGCEKKVRRAISKLDGVDTVEIDVDRQKVTVTGYVDREEVLKMVKRTGRT 76

Query: 66 A 67
          A
Sbjct: 77 A 77

BLAST of MS014645 vs. TAIR 10
Match: AT1G29100.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 3.7e-08
Identity = 26/68 (38.24%), Postives = 44/68 (64.71%), Query Frame = 0

Query: 9  IKVRMDCNGCVQKIKKALYGINGIYDLYIDFPQQKLTIIGWADPDKIMKAIKK-TRKIAT 68
          ++V MDC GC  K++KAL  +NG++D+ ID  QQ++T+ G A+  K++K  +  T++   
Sbjct: 1  MEVPMDCPGCENKVRKALEKMNGVHDVQIDIKQQRVTVTGSAEQKKVLKVARNVTKRDIC 60

Query: 69 ICSDPEQP 76
          + S P  P
Sbjct: 61 LWSYPYHP 68