MS014643 (gene) Bitter gourd (TR) v1

Overview


Name	MS014643
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Chaperone protein DnaJ
Location	scaffold1096: 282282 .. 283492 (+)
RNA-Seq Expression	MS014643
Synteny	MS014643

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCGCGAATCCGATTTCAAATCCCATTTGGCCTCAGAAATCTGCTCCATCTCTGCCCGCACCGTTGCCTGCCCTCACCGCCATTACTGCCCCTCTATCCCCTCCCCTTTCATCGATTGGTATCGTCTTCTCGGGGTACGTTTTGGGTTTTCCCCCCATTTCCTCTCTGGGTTTTGTTTTTTTTTATTTGTTTCTTTGTTAATGGGAACCTAATAGGTCGAAGAAGATTCAGGAATCGATGTTATTCGGAGGGCGTATCTTAAGCTTGGTGGGGTTTCCATTTTACTCTCTCCATTGCGATTTTGATTTTTTTTATTGGAATTTTGAAATGCGCTTGGTTTTTTGTTTCTTTTGCAGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGTTCGATTCTTTCATTTCTCTTCTTTGGGAATCTAAATCCATGGCTGCAAAAATTCATTATGCGTTTCACCGATCACTGTTGTTCTGCAACTGCTTGTGCATTTAAGTTCTTCAATCATATAGTACTCGTGATCTTGATGAATGCTCTGATTTCAGTTTAATGAAAATAAATTGAGAGGTAGATACAGTACAGAGAAGTTTGTGAGTGAGTGAGTGATTGTTGTGTATTTGTAGGGATATGCGTGTTTAAGTGACAATGTGAAGAGAAGGGCGTTTGATTTGGATAGGAAGAAGAAATTCTGCCCAGATTGCAAGACAATCCCTTACAGAAATCTGAGCTCTGCTAGAAAGTTGAATCCCTTGAATGTATTAGGAGCTTTGAAAGTTATGAAAGAGAGATTGAAAGAGGAGGCAAGTGTGATTGAGAATTGTTTGAGGGCTAATAATAATAATACTTCTAATTCATCAGCAACCACAGAAACTACTTCTTCTTCTTCATCAATCTTCAACCCACCACCAGGAGCTTGGTTTCACCACAGAAACCTCAAAGAAACCCCAATTTTCAACCCTTCTGATTATGTATGCCAAGGCTACCCCCATTTCCGGACCCGAATCCACTCGAAACCGCAGAGTTTTCGGTGTTTTAGAACCGGGAACACGCTCAAGTATGAACAGGCTAGAGGGAAGTATGAGCACCCAGTGTTTGAGGTTAGATCAGAAAGCTTTCTTTTACAGAAACAATCTGCTTTTGTTTGTTCTTCAAATGTTAACAGATCACATTGG

mRNA sequence

ATGGGTCGCGAATCCGATTTCAAATCCCATTTGGCCTCAGAAATCTGCTCCATCTCTGCCCGCACCGTTGCCTGCCCTCACCGCCATTACTGCCCCTCTATCCCCTCCCCTTTCATCGATTGGTATCGTCTTCTCGGGGTCGAAGAAGATTCAGGAATCGATGTTATTCGGAGGGCGTATCTTAAGCTTGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGCGTGTTTAAGTGACAATGTGAAGAGAAGGGCGTTTGATTTGGATAGGAAGAAGAAATTCTGCCCAGATTGCAAGACAATCCCTTACAGAAATCTGAGCTCTGCTAGAAAGTTGAATCCCTTGAATGTATTAGGAGCTTTGAAAGTTATGAAAGAGAGATTGAAAGAGGAGGCAAGTGTGATTGAGAATTGTTTGAGGGCTAATAATAATAATACTTCTAATTCATCAGCAACCACAGAAACTACTTCTTCTTCTTCATCAATCTTCAACCCACCACCAGGAGCTTGGTTTCACCACAGAAACCTCAAAGAAACCCCAATTTTCAACCCTTCTGATTATGTATGCCAAGGCTACCCCCATTTCCGGACCCGAATCCACTCGAAACCGCAGAGTTTTCGGTGTTTTAGAACCGGGAACACGCTCAAGTATGAACAGGCTAGAGGGAAGTATGAGCACCCAGTGTTTGAGGTTAGATCAGAAAGCTTTCTTTTACAGAAACAATCTGCTTTTGTTTGTTCTTCAAATGTTAACAGATCACATTGG

Coding sequence (CDS)

ATGGGTCGCGAATCCGATTTCAAATCCCATTTGGCCTCAGAAATCTGCTCCATCTCTGCCCGCACCGTTGCCTGCCCTCACCGCCATTACTGCCCCTCTATCCCCTCCCCTTTCATCGATTGGTATCGTCTTCTCGGGGTCGAAGAAGATTCAGGAATCGATGTTATTCGGAGGGCGTATCTTAAGCTTGCTCTGCAACTTCATCCAGACAAAAACTCGCACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGCGTGTTTAAGTGACAATGTGAAGAGAAGGGCGTTTGATTTGGATAGGAAGAAGAAATTCTGCCCAGATTGCAAGACAATCCCTTACAGAAATCTGAGCTCTGCTAGAAAGTTGAATCCCTTGAATGTATTAGGAGCTTTGAAAGTTATGAAAGAGAGATTGAAAGAGGAGGCAAGTGTGATTGAGAATTGTTTGAGGGCTAATAATAATAATACTTCTAATTCATCAGCAACCACAGAAACTACTTCTTCTTCTTCATCAATCTTCAACCCACCACCAGGAGCTTGGTTTCACCACAGAAACCTCAAAGAAACCCCAATTTTCAACCCTTCTGATTATGTATGCCAAGGCTACCCCCATTTCCGGACCCGAATCCACTCGAAACCGCAGAGTTTTCGGTGTTTTAGAACCGGGAACACGCTCAAGTATGAACAGGCTAGAGGGAAGTATGAGCACCCAGTGTTTGAGGTTAGATCAGAAAGCTTTCTTTTACAGAAACAATCTGCTTTTGTTTGTTCTTCAAATGTTAACAGATCACATTGG

Protein sequence

MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLGALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYEHPVFEVRSESFLLQKQSAFVCSSNVNRSHW

Homology

[Close]

BLAST of MS014643 vs. NCBI nr
Match: XP_022137924.1 (uncharacterized protein LOC111009220, partial [Momordica charantia])

HSP 1 Score: 398.3 bits (1022), Expect = 5.3e-107
Identity = 194/196 (98.98%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 74  HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG 133
           HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG
Sbjct: 1   HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG 60

Query: 134 ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET 193
           ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET
Sbjct: 61  ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET 120

Query: 194 PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYEHPVFEVRSESFLLQ 253
           PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYE PVFEVRSESFLLQ
Sbjct: 121 PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYEQPVFEVRSESFLLQ 180

Query: 254 KQSAFVCSSNVNRSHW 270
           KQSAF CSSNVNRSHW
Sbjct: 181 KQSAFFCSSNVNRSHW 196

[Close]

BLAST of MS014643 vs. NCBI nr
Match: TYK16200.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 395.6 bits (1015), Expect = 3.5e-106
Identity = 201/280 (71.79%), Postives = 221/280 (78.93%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHLA EI S S+RTV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV--RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+  +SESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

[Close]

BLAST of MS014643 vs. NCBI nr
Match: XP_038902437.1 (uncharacterized protein LOC120089081 [Benincasa hispida])

HSP 1 Score: 394.4 bits (1012), Expect = 7.7e-106
Identity = 202/280 (72.14%), Postives = 224/280 (80.00%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHLA+EI S S+RTV+C HRH+      PFIDWYRL GV++D+GID IRR Y
Sbjct: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHF----SLPFIDWYRLFGVQQDAGIDSIRRRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRR FDLDRKKKFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPL----------NVLGALKVMKERLKEEASVIENCLRANNNNTSNSSATTETT 180
           +SAR LN L          N+ G L+ MKERL+EEA+VI+NCLR     T+NS+A  ET 
Sbjct: 121 TSARNLNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLR-----TNNSAANRET- 180

Query: 181 SSSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLK 240
               S+FNPP    FH++N KETPIFNPSDYVCQGYPHFRTRIH KPQSFRCFRTGN LK
Sbjct: 181 ----SLFNPPGFTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALK 240

Query: 241 YEQARGKYEHPVFEV-RSESFLLQKQSAFVCSSNVNRSHW 270
           YEQ RGKYE+PVFE+ RSESFLLQKQSAFV  SNVN+ HW
Sbjct: 241 YEQGRGKYEYPVFEISRSESFLLQKQSAFV-YSNVNKPHW 265

[Close]

BLAST of MS014643 vs. NCBI nr
Match: KAA0049521.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 394.0 bits (1011), Expect = 1.0e-105
Identity = 200/280 (71.43%), Postives = 220/280 (78.57%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHL  EI S S+RTV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV--RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+  +SESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

[Close]

BLAST of MS014643 vs. NCBI nr
Match: XP_008438915.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-105
Identity = 201/281 (71.53%), Postives = 220/281 (78.29%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHLA EI S S+ TV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV---RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+   RSESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

[Close]

BLAST of MS014643 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-13
Identity = 33/62 (53.23%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+E++  +D IR+AY KL+L++HPDKN  P +E AFK VS+ + CLSD   RR 
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 100 FD 102
           FD
Sbjct: 159 FD 160

[Close]

BLAST of MS014643 vs. ExPASy Swiss-Prot
Match: P0CW07 (Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.9e-12
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+ +DS ++ I++ Y KLALQ HPD+N  P AE  FK +SE YA LSD  KR  
Sbjct: 6   DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 100 FD 102
           +D
Sbjct: 66  YD 67

[Close]

BLAST of MS014643 vs. ExPASy Swiss-Prot
Match: P0CW06 (Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.9e-12
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+ +DS ++ I++ Y KLALQ HPD+N  P AE  FK +SE YA LSD  KR  
Sbjct: 6   DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 100 FD 102
           +D
Sbjct: 66  YD 67

[Close]

BLAST of MS014643 vs. ExPASy Swiss-Prot
Match: Q8TQR1 (Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 8.8e-12
Identity = 31/62 (50.00%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+ +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +SE YA LSD  KR  
Sbjct: 6   DYYEILGLPKDASVEDIKKTYRKLALQYHPDRNKDPGAEDKFKEISEAYAVLSDTEKRAQ 65

Query: 100 FD 102
           +D
Sbjct: 66  YD 67

[Close]

BLAST of MS014643 vs. ExPASy Swiss-Prot
Match: Q24133 (DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-11
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y++LG+E  +  D I++AY KLAL+ HPDKN  P+AE  FK ++E Y  LSD  KR  
Sbjct: 4   DFYKILGLERKASDDEIKKAYRKLALKYHPDKNKSPQAEERFKEIAEAYEVLSDKKKRDI 63

Query: 100 FD 102
           FD
Sbjct: 64  FD 65

[Close]

BLAST of MS014643 vs. ExPASy TrEMBL
Match: A0A6J1C8M6 (uncharacterized protein LOC111009220 OS=Momordica charantia OX=3673 GN=LOC111009220 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.6e-107
Identity = 194/196 (98.98%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 74  HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG 133
           HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG
Sbjct: 1   HPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARKLNPLNVLG 60

Query: 134 ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET 193
           ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET
Sbjct: 61  ALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWFHHRNLKET 120

Query: 194 PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYEHPVFEVRSESFLLQ 253
           PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYE PVFEVRSESFLLQ
Sbjct: 121 PIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKYEQARGKYEQPVFEVRSESFLLQ 180

Query: 254 KQSAFVCSSNVNRSHW 270
           KQSAF CSSNVNRSHW
Sbjct: 181 KQSAFFCSSNVNRSHW 196

[Close]

BLAST of MS014643 vs. ExPASy TrEMBL
Match: A0A5D3CXW5 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001280 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.7e-106
Identity = 201/280 (71.79%), Postives = 221/280 (78.93%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHLA EI S S+RTV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV--RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+  +SESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

[Close]

BLAST of MS014643 vs. ExPASy TrEMBL
Match: A0A5A7U7H9 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007750 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 4.9e-106
Identity = 200/280 (71.43%), Postives = 220/280 (78.57%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHL  EI S S+RTV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV--RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+  +SESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

[Close]

BLAST of MS014643 vs. ExPASy TrEMBL
Match: A0A1S3AXH7 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.4e-106
Identity = 201/281 (71.53%), Postives = 220/281 (78.29%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG +SDFKSHLA EI S S+ TV+CPHRH+ P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFCPDC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNF 120

Query: 121 SSARKLNPLNVL---------GALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LN+          G L+ MKERL+EEA+VIENCLRAN     NSS+TTET  
Sbjct: 121 TSATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRAN-----NSSSTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP    FHHRN KETPIFNPSDYVCQGYPHFRTRIH K +SFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV---RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGKYE+PVFE+   RSESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKYEYPVFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

[Close]

BLAST of MS014643 vs. ExPASy TrEMBL
Match: A0A0A0L5B2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.8e-104
Identity = 201/281 (71.53%), Postives = 219/281 (77.94%), Query Frame = 0


Query: 1   MGRESDFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAY 60
           MG  SDFKSHLASEI S S+ TV+CPHR + P    PFIDWYRL GV++D+ ID IR  Y
Sbjct: 1   MGPHSDFKSHLASEISSFSSLTVSCPHRLFSP----PFIDWYRLFGVQQDAPIDFIRSRY 60

Query: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNL 120
           LKLALQLHPDKNSHPKAEIAFKLVSEGY CLSDNVKRRAFDLDRK KFC DC TIPYRN 
Sbjct: 61  LKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNY 120

Query: 121 SSARKLNPLNVLGA---------LKVMKERLKEEASVIENCLRANNNNTSNSSATTETTS 180
           +SA  LN LNVLG+         L+ +KERL+EEA+VIE CLRAN     NSS TTET  
Sbjct: 121 TSATNLNALNVLGSFHNNIHGRGLRDVKERLREEANVIEYCLRAN-----NSSTTTET-- 180

Query: 181 SSSSIFNPPPGAWFHHRNLKETPIFNPSDYVCQGYPHFRTRIHSKPQSFRCFRTGNTLKY 240
              S+FNPP  + FHHRN KETPIFNPSDYVCQGYPHFRTRIH KPQSFRCFR+GN LKY
Sbjct: 181 ---SLFNPPGCSMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKY 240

Query: 241 EQARGKYEHPVFEV---RSESFLLQKQSAFVCSSNVNRSHW 270
           EQ RGK E+PVFE+   RSESFLLQKQSAFV S+    SHW
Sbjct: 241 EQGRGKCEYPVFEISSNRSESFLLQKQSAFVYSNVNKSSHW 267

[Close]

BLAST of MS014643 vs. TAIR 10
Match: AT3G06778.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-40
Identity = 84/208 (40.38%), Postives = 128/208 (61.54%), Query Frame = 0


Query: 6   DFKSHLASEICSISARTVACPHRHYCPSIPSPFIDWYRLLGVEEDSGIDVIRRAYLKLAL 65
           D K+ L S+I ++S   ++C H +   S  S FIDWY +LG++ED+ + VIR+ Y KLAL
Sbjct: 9   DPKAALVSDILALSRSPISCIHINRISS-GSCFIDWYLILGIQEDAEVKVIRKRYHKLAL 68

Query: 66  QLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRAFDLDRKKKFCPDCKTIPYRNLSSARK 125
           ++HPDKN+HPKA+IAFKL+ E Y CLSD  KRR+F++DR+   C  C  + +++  +   
Sbjct: 69  KVHPDKNNHPKADIAFKLIHEAYLCLSDETKRRSFNIDRRNNICLKCSRVSHKSKENRND 128

Query: 126 LNPLNVLGALKVMKERLKEEASVIENCLRANNNNTSNSSATTETTSSSSSIFNPPPGAWF 185
             P      LK ++++ +EE  VIE CL+     T+++      T+ +  ++  P     
Sbjct: 129 SKPNRFCQTLKDIRDKFREENMVIERCLK-----TNSAFFMGNRTNETPPVYGIPK---- 188

Query: 186 HHRNLKETPIFNPSDYVCQGYPHFRTRI 214
            +R  KE+P+FNPSDY   GYPH R R+
Sbjct: 189 QNRINKESPVFNPSDYRLWGYPHVRNRV 206

[Close]

BLAST of MS014643 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 77.0 bits (188), Expect = 2.5e-14
Identity = 33/62 (53.23%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+E++  +D IR+AY KL+L++HPDKN  P +E AFK VS+ + CLSD   RR 
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 100 FD 102
           FD
Sbjct: 159 FD 160

[Close]

BLAST of MS014643 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 72.4 bits (176), Expect = 6.2e-13
Identity = 29/63 (46.03%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+E +  +D +R+AY KL+L++HPDKN  P +E AFK VS+ + CLS++  R+ 
Sbjct: 113 DYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKK 172

Query: 100 FDL 103
           +D+
Sbjct: 173 YDV 175

[Close]

BLAST of MS014643 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 72.4 bits (176), Expect = 6.2e-13
Identity = 29/63 (46.03%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           D+Y +LG+E +  +D +R+AY KL+L++HPDKN  P +E AFK VS+ + CLS++  R+ 
Sbjct: 113 DYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKK 172

Query: 100 FDL 103
           +D+
Sbjct: 173 YDV 175

[Close]

BLAST of MS014643 vs. TAIR 10
Match: AT5G18750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-12
Identity = 34/69 (49.28%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 40  DWYRLLGVEEDSGIDVIRRAYLKLALQLHPDKNSHPKAEIAFKLVSEGYACLSDNVKRRA 99
           DWY++L VE+ +  + I++ Y KLAL LHPDKN  P AE AFK + E    L D  KRR 
Sbjct: 66  DWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGEAQRVLLDKDKRRF 125

Query: 100 FDLDRKKKF 109
            D+ RK  F
Sbjct: 126 HDMRRKPVF 134

The following BLAST results are available for this feature:

BLAST of MS014643 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137924.1	5.3e-107	98.98	uncharacterized protein LOC111009220, partial [Momordica charantia]	[more]
TYK16200.1	3.5e-106	71.79	chaperone protein DnaJ [Cucumis melo var. makuwa]	[more]
XP_038902437.1	7.7e-106	72.14	uncharacterized protein LOC120089081 [Benincasa hispida]	[more]
KAA0049521.1	1.0e-105	71.43	chaperone protein DnaJ [Cucumis melo var. makuwa]	[more]
XP_008438915.1	1.3e-105	71.53	PREDICTED: chaperone protein DnaJ [Cucumis melo]	[more]

BLAST of MS014643 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FH28	3.6e-13	53.23	Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2	[more]
P0CW07	3.9e-12	51.61	Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 /...	[more]
P0CW06	3.9e-12	51.61	Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1	[more]
Q8TQR1	8.8e-12	50.00	Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 28...	[more]
Q24133	1.5e-11	51.61	DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3	[more]

BLAST of MS014643 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8M6	2.6e-107	98.98	uncharacterized protein LOC111009220 OS=Momordica charantia OX=3673 GN=LOC111009...	[more]
A0A5D3CXW5	1.7e-106	71.79	Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A5A7U7H9	4.9e-106	71.43	Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1...	[more]
A0A1S3AXH7	6.4e-106	71.53	chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1	[more]
A0A0A0L5B2	7.8e-104	71.53	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1	[more]

BLAST of MS014643 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06778.1	2.1e-40	40.38	Chaperone DnaJ-domain superfamily protein	[more]
AT5G49060.1	2.5e-14	53.23	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT3G57340.1	6.2e-13	46.03	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT3G57340.2	6.2e-13	46.03	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT5G18750.1	1.1e-12	49.28	DNAJ heat shock N-terminal domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 42..60 score: 37.93 coord: 60..75 score: 50.47 coord: 76..96 score: 42.54
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 39..96 e-value: 9.1E-18 score: 75.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 40..101 e-value: 1.1E-18 score: 67.1
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 40..104 score: 16.021288
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 40..93 e-value: 1.99191E-17 score: 72.1929
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 36..114 e-value: 9.4E-21 score: 75.8
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 32..103
None	No IPR available	PANTHER	PTHR44137	BNAC03G44070D PROTEIN	coord: 4..250
None	No IPR available	PANTHER	PTHR44137:SF13	CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN	coord: 4..250
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 81..100

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014643.1	MS014643.1	mRNA