MS014636 (gene) Bitter gourd (TR) v1

Overview


Name	MS014636
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Epoxide hydrolase-like
Location	scaffold1096: 213444 .. 215771 (-)
RNA-Seq Expression	MS014636
Synteny	MS014636

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCATTGAAATTGAACCGATGTGTCGGTTTTCTTGGCAGTTTCTTCGCGGACTGTTCAATTCTCCGCCTCTCTCATCGCCTATAAGAGCAGGGCCTAAGCAGAATCAAACTGAAACAGCAAAATCAAAAATGGAGAAGGAGAGCGGAATGGAGAGCATACGCCACAGCACCGTGAGCGTGAACGGAATCAATATGCACGTGGCGGAGAAAGGCGAAGGTCCGGTGGTCCTCTTCATCCATGGCTTCCCGGAGCTCTGGTACTCGTGGCGGCACCAGATCCTGGATCTGGCCTCGCGCGGCTACCGCGCCGTCGCTCCGGACCTCCGCGGCTACGGAGACTCCGACGCGCCGCCGTCGCAGAGCGGCTACACGAGCTTCCACATAGTAGGGGACTTGGTGGCGCTGATCGACGCCATTGTTGGGGCGGAGGAGAAGGTTTTCGTTGTGGCGCATGATTGGGGGGCCATCATTGCTTGGTACCTTTGCCTGTATCGGCCGGATCGGATTAGGGCGTTGGTGAATATGAGCGTCGCGTTCACTCGGAGAAGCCCTAAGAGGAAGCCGATCGAATCGCTTAGAGCTCTCTATGGCGACGATTATTACATTTGCAGATTTCAGGTATATTTTTTTTTTCCTTTCTTCACTCATTTTCGACTGCCGTTACAATTTCAGAGTTTCGTTTTTTTTTTCTCTCTTTGGAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNCGGCCGTTATAATTTAGCGTAGAGTTGTATGGTTGTGGTTGGTTGGGATGAACTCTTCACTACTGCATAACAGACTGCCAGGTGGCCATATACAAAGTCGACACTTGTTAAAAGTTTTTTTTTTCCTTTTCTATGAGGTGATGATGTTAAATACAGAAGTATTTTAATTTGATATTTTATTTTTATAATTATTTTTAAATGTTTAAGTGTTGAATACTCCTACAAACTTGAGCAAAAATTACTTGGCTTAAATTTTACACTCAATCATAACTAAGGTCCGTTTGATAAATATTTTTGTTTTTGGTTTCCTTTTACTTTTTATTTTTAAAAAACTAGAAAACCCAACCAAACATGTTGGTAACCTTTTTTTAGTTTTCTTTCTAAAAGATTATAAATTTGAAAATATATGTTAAAAGAAAAAAAAATTAAGAGAAAAGAAAATATAGTGAGAAAAAACTTTAGACATAAAAACAATATTTGAAATAGAGAAAATAAATAAATACAAAAATAAATAAAAGTTATAGTAGAGAAAATTAATCAATTTGGAGGAAGGGAGACATAATAATAAATATTTATGAAGAATAAAAATTTAGAGTGAAAAAGAAAGTTGTTAGAGATAAATATGTCTAGGAAGAAAATGAGATTTTGCAAAATAGAAATCTGTTTTAAATTTATTTTTTTTTTACATAAAATTTCAAAATTCGTTTTTTTAAATTTACATGAAATTTCAAAAATAGTTTTTTGGTTTTTTTTTTTGAATCTAAAAAATGATTTATTCTCAGTGGTTAAAAAAGTATATTTTTTCTCCATCTCCAAAACTAGAACTGTGTAAAGTCCCCCACTCTATTTTAGGGGTGCCAGCTGAAGCTGGACAACCCAGTTGAATCAAAGCAATTCCACTCAATTTGATCTAAAAAATGATCATTCTTGGATAAAAAAAAAAAAAAGAAAATATTTATCTTAACGCAGAGGAAAACAATTTAAAACAAAGAAATTGGAAATGGAATTGTGATGCAGGAACCTGGAGAAATCGAGGCCGAGTTTGCTAAGATTGGCACTGAAAGAGTTATGACTGAATTTCTTACTTACCATACTCCAAAGCCACTGATGCTTCCCAAAGGCAAAGGCTTCGGCCACCCACTGGACACTCCTATTCCTTTGCCTCCATGGTTGTCACACCAAGACATTCAATATTACACCTCCAAATTCGACACCAAAGGCTTCACTGCCCCGATCAACTACTACCGAAATCTCGACAGGTGCGCTCATTTTATTATCATATATCACATACCTTACACCGCATTTCGGTCGCAGGGTCTCATAAAATATAGCTGCAATGCTCCTGTTGCAGGAACTGGGAACTCAATGCTCCATTCACAGGGGCTCAAGTGAAAGTTCCAGTGAAGTTCATTGTGGGTGACCTTGACCTTTCCTACAATTCTTTCGGAACTAAGGAATATATAAACAGTGGGGAGATGAAGAAAGATGTGCCCTTCCTTGAAGAGGTCGTTGTGATGGAAGGCGTAGGCCACTTCCTCCAAGAGGAGAAGCCCCGCGAGATCAGCGACCACATCTATCAATTCATTACAAAGTTC

mRNA sequence

CGCATTGAAATTGAACCGATGTGTCGGTTTTCTTGGCAGTTTCTTCGCGGACTGTTCAATTCTCCGCCTCTCTCATCGCCTATAAGAGCAGGGCCTAAGCAGAATCAAACTGAAACAGCAAAATCAAAAATGGAGAAGGAGAGCGGAATGGAGAGCATACGCCACAGCACCGTGAGCGTGAACGGAATCAATATGCACGTGGCGGAGAAAGGCGAAGGTCCGGTGGTCCTCTTCATCCATGGCTTCCCGGAGCTCTGGTACTCGTGGCGGCACCAGATCCTGGATCTGGCCTCGCGCGGCTACCGCGCCGTCGCTCCGGACCTCCGCGGCTACGGAGACTCCGACGCGCCGCCGTCGCAGAGCGGCTACACGAGCTTCCACATAGTAGGGGACTTGGTGGCGCTGATCGACGCCATTGTTGGGGCGGAGGAGAAGGTTTTCGTTGTGGCGCATGATTGGGGGGCCATCATTGCTTGGTACCTTTGCCTGTATCGGCCGGATCGGATTAGGGCGTTGGTGAATATGAGCGTCGCGTTCACTCGGAGAAGCCCTAAGAGGAAGCCGATCGAATCGCTTAGAGCTCTCTATGGCGACGATTATTACATTTGCAGATTTCAGGAACCTGGAGAAATCGAGGCCGAGTTTGCTAAGATTGGCACTGAAAGAGTTATGACTGAATTTCTTACTTACCATACTCCAAAGCCACTGATGCTTCCCAAAGGCAAAGGCTTCGGCCACCCACTGGACACTCCTATTCCTTTGCCTCCATGGTTGTCACACCAAGACATTCAATATTACACCTCCAAATTCGACACCAAAGGCTTCACTGCCCCGATCAACTACTACCGAAATCTCGACAGGAACTGGGAACTCAATGCTCCATTCACAGGGGCTCAAGTGAAAGTTCCAGTGAAGTTCATTGTGGGTGACCTTGACCTTTCCTACAATTCTTTCGGAACTAAGGAATATATAAACAGTGGGGAGATGAAGAAAGATGTGCCCTTCCTTGAAGAGGTCGTTGTGATGGAAGGCGTAGGCCACTTCCTCCAAGAGGAGAAGCCCCGCGAGATCAGCGACCACATCTATCAATTCATTACAAAGTTC

Coding sequence (CDS)

CGCATTGAAATTGAACCGATGTGTCGGTTTTCTTGGCAGTTTCTTCGCGGACTGTTCAATTCTCCGCCTCTCTCATCGCCTATAAGAGCAGGGCCTAAGCAGAATCAAACTGAAACAGCAAAATCAAAAATGGAGAAGGAGAGCGGAATGGAGAGCATACGCCACAGCACCGTGAGCGTGAACGGAATCAATATGCACGTGGCGGAGAAAGGCGAAGGTCCGGTGGTCCTCTTCATCCATGGCTTCCCGGAGCTCTGGTACTCGTGGCGGCACCAGATCCTGGATCTGGCCTCGCGCGGCTACCGCGCCGTCGCTCCGGACCTCCGCGGCTACGGAGACTCCGACGCGCCGCCGTCGCAGAGCGGCTACACGAGCTTCCACATAGTAGGGGACTTGGTGGCGCTGATCGACGCCATTGTTGGGGCGGAGGAGAAGGTTTTCGTTGTGGCGCATGATTGGGGGGCCATCATTGCTTGGTACCTTTGCCTGTATCGGCCGGATCGGATTAGGGCGTTGGTGAATATGAGCGTCGCGTTCACTCGGAGAAGCCCTAAGAGGAAGCCGATCGAATCGCTTAGAGCTCTCTATGGCGACGATTATTACATTTGCAGATTTCAGGAACCTGGAGAAATCGAGGCCGAGTTTGCTAAGATTGGCACTGAAAGAGTTATGACTGAATTTCTTACTTACCATACTCCAAAGCCACTGATGCTTCCCAAAGGCAAAGGCTTCGGCCACCCACTGGACACTCCTATTCCTTTGCCTCCATGGTTGTCACACCAAGACATTCAATATTACACCTCCAAATTCGACACCAAAGGCTTCACTGCCCCGATCAACTACTACCGAAATCTCGACAGGAACTGGGAACTCAATGCTCCATTCACAGGGGCTCAAGTGAAAGTTCCAGTGAAGTTCATTGTGGGTGACCTTGACCTTTCCTACAATTCTTTCGGAACTAAGGAATATATAAACAGTGGGGAGATGAAGAAAGATGTGCCCTTCCTTGAAGAGGTCGTTGTGATGGAAGGCGTAGGCCACTTCCTCCAAGAGGAGAAGCCCCGCGAGATCAGCGACCACATCTATCAATTCATTACAAAGTTC

Protein sequence

RIEIEPMCRFSWQFLRGLFNSPPLSSPIRAGPKQNQTETAKSKMEKESGMESIRHSTVSVNGINMHVAEKGEGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREISDHIYQFITKF

Homology

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BLAST of MS014636 vs. NCBI nr
Match: XP_008438903.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 579.7 bits (1493), Expect = 1.8e-161
Identity = 275/371 (74.12%), Postives = 309/371 (83.29%), Query Frame = 0


Query: 1   RIEIEPMCRFSWQFLRGLFNSPPLSSPIRAGPKQNQTETAKSKMEKESGMESIRHSTVSV 60
           ++  E M      FL  LFN   LS PI   P     +T   KME +  + SIRH T++V
Sbjct: 2   KVTFESMSTIFRHFLSKLFNFHMLSPPIIQSP---NPQTHSPKMENQMELSSIRHRTLNV 61

Query: 61  NGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPS 120
           NGIN+H+AEKGE GP++LFIHGFPELWYSWR+QILDL+SRGYRAVAPDLRGYGDSD+PPS
Sbjct: 62  NGINLHIAEKGESGPLILFIHGFPELWYSWRYQILDLSSRGYRAVAPDLRGYGDSDSPPS 121

Query: 121 QSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAF 180
            + YT FHIVGDL+ALIDAIVG EEKVFVV HDWGA+IAW LC+YRPDR++ALVN SV F
Sbjct: 122 VNDYTCFHIVGDLIALIDAIVGVEEKVFVVGHDWGAMIAWNLCMYRPDRVKALVNTSVTF 181

Query: 181 TRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLP 240
            RRSPKRKPI +L+ALYGDDYYICRFQEPGEIEAEFA+IGTER+MTE L+Y  PKPLM+P
Sbjct: 182 NRRSPKRKPIPALKALYGDDYYICRFQEPGEIEAEFAEIGTERIMTEILSYRIPKPLMMP 241

Query: 241 KGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTG 300
           KG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PINYYRNLDRNWELNAPFTG
Sbjct: 242 KGRGKGKDHPLDTPISLPPWLTKQDMDYYVSKFDKNGFTGPINYYRNLDRNWELNAPFTG 301

Query: 301 AQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREI 360
           AQVKVP KFIVGD DL+YNSFG K+YI SGEMKKDVPFLEEVVVMEGVGHFLQEEKP EI
Sbjct: 302 AQVKVPTKFIVGDQDLTYNSFGAKQYIESGEMKKDVPFLEEVVVMEGVGHFLQEEKPHEI 361

Query: 361 SDHIYQFITKF 369
           S+HIY+FI  +
Sbjct: 362 SNHIYEFIKNY 369

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BLAST of MS014636 vs. NCBI nr
Match: XP_004134492.1 (uncharacterized protein LOC101212443 [Cucumis sativus] >KGN57130.1 hypothetical protein Csa_011699 [Cucumis sativus])

HSP 1 Score: 564.7 bits (1454), Expect = 5.9e-157
Identity = 259/322 (80.43%), Postives = 291/322 (90.37%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDL 109
           + SIRH+T+++NGIN+H+AEKGE GP++LFIHGFPELWYSWRHQILDL+SRGYRAVAPDL
Sbjct: 3   LSSIRHTTLNLNGINLHIAEKGESGPLILFIHGFPELWYSWRHQILDLSSRGYRAVAPDL 62

Query: 110 RGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDR 169
           RGYGDSD+PPS + YT FHIVGDL+ALIDA+VG EEKVFVV HDWGA+IAW LC+YRPDR
Sbjct: 63  RGYGDSDSPPSVNDYTCFHIVGDLIALIDALVGVEEKVFVVGHDWGAVIAWNLCMYRPDR 122

Query: 170 IRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFL 229
           ++ALVN SV F RRSPKRKPIESL+ALYGDDYYICRFQEPGEIEAEFA+IGTER+MTE L
Sbjct: 123 VKALVNTSVTFNRRSPKRKPIESLKALYGDDYYICRFQEPGEIEAEFAEIGTERIMTEIL 182

Query: 230 TYHTPKPLMLPKGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLD 289
           +Y TPKPLM+PKG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PINYYRNLD
Sbjct: 183 SYRTPKPLMMPKGRGKGKDHPLDTPISLPPWLAKQDMDYYVSKFDKNGFTGPINYYRNLD 242

Query: 290 RNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVG 349
           RNWELNA FTGAQVKVP KFIVGD DL+Y+SFG K+YI SGEMKKDVPFLEEVVVMEGVG
Sbjct: 243 RNWELNASFTGAQVKVPTKFIVGDQDLTYHSFGAKQYIQSGEMKKDVPFLEEVVVMEGVG 302

Query: 350 HFLQEEKPREISDHIYQFITKF 369
           HFLQEEKP EIS+HIY+FI  +
Sbjct: 303 HFLQEEKPHEISNHIYEFIKNY 324

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BLAST of MS014636 vs. NCBI nr
Match: KAA0049512.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK16192.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 564.7 bits (1454), Expect = 5.9e-157
Identity = 261/328 (79.57%), Postives = 292/328 (89.02%), Query Frame = 0


Query: 44  MEKESGMESIRHSTVSVNGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYR 103
           ME +  + SIRH T++VNGIN+H+AEKGE GP++LFIHGFPELWYSWR+QILDL+SRGYR
Sbjct: 1   MENQMELSSIRHRTLNVNGINLHIAEKGESGPLILFIHGFPELWYSWRYQILDLSSRGYR 60

Query: 104 AVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLC 163
           AVAPDLRGYGDSD+PPS + YT FHIVGDL+ALIDAIVG EEKVFVV HDWGA+IAW LC
Sbjct: 61  AVAPDLRGYGDSDSPPSVNDYTCFHIVGDLIALIDAIVGVEEKVFVVGHDWGAMIAWNLC 120

Query: 164 LYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTER 223
           +YRPDR++ALVN SV F RRSPKRKPI +L+ALYGDDYYICRFQEPGEIEAEFA+IGTER
Sbjct: 121 MYRPDRVKALVNTSVTFNRRSPKRKPIPALKALYGDDYYICRFQEPGEIEAEFAEIGTER 180

Query: 224 VMTEFLTYHTPKPLMLPKGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPIN 283
           +MTE L+Y  PKPLM+PKG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PIN
Sbjct: 181 IMTEILSYRIPKPLMMPKGRGKGKDHPLDTPISLPPWLTKQDMDYYVSKFDKNGFTGPIN 240

Query: 284 YYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVV 343
           YYRNLDRNWELNAPFTGAQVKVP KFIVGD DL+YNSFG K+YI SGEMKKDVPFLEEVV
Sbjct: 241 YYRNLDRNWELNAPFTGAQVKVPTKFIVGDQDLTYNSFGAKQYIESGEMKKDVPFLEEVV 300

Query: 344 VMEGVGHFLQEEKPREISDHIYQFITKF 369
           VMEGVGHFLQEEKP EIS+HIY+FI  +
Sbjct: 301 VMEGVGHFLQEEKPHEISNHIYEFIKNY 328

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BLAST of MS014636 vs. NCBI nr
Match: XP_038894580.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.4e-155
Identity = 270/346 (78.03%), Postives = 299/346 (86.42%), Query Frame = 0


Query: 10  FSWQFLRGLFNSPPLSSPIRAGPKQNQTETAKSKMEK-ESGMESIRHSTVSVNGINMHVA 69
           F  QFLR +F+ P  + PI    + +  +T  SKME  +  + +I H T+SVNGIN+HVA
Sbjct: 20  FFRQFLRKMFDFPIPTPPI---IESSDHQTESSKMENYQIEIATIHHRTLSVNGINLHVA 79

Query: 70  EKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFH 129
           EKGE GP++LFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSD+PPS + YT FH
Sbjct: 80  EKGESGPLILFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDSPPSVTDYTCFH 139

Query: 130 IVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRK 189
           IVGDL+ALIDAIVG EEKVFVVAHDWGA+IAW LCLYRPDRIRALVN+SV F RRSPKRK
Sbjct: 140 IVGDLIALIDAIVGVEEKVFVVAHDWGAVIAWNLCLYRPDRIRALVNLSVTFNRRSPKRK 199

Query: 190 PIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPK--GKGFG 249
           PIESLRALYGDDYYICRFQEPGEIEAEF ++GTER+MTE L+Y  PKPLM+PK  G+G G
Sbjct: 200 PIESLRALYGDDYYICRFQEPGEIEAEFDEVGTERIMTEILSYRIPKPLMMPKGRGRGKG 259

Query: 250 HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVK 309
           HPLDTP PLPPWL+ QD+ YY SKFD  GFT PINYYRNLDRNWELNAPFTGAQVKVP K
Sbjct: 260 HPLDTPNPLPPWLTQQDMDYYVSKFDKNGFTGPINYYRNLDRNWELNAPFTGAQVKVPTK 319

Query: 310 FIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQE 352
           FIVGDLDL+YNSFG K+YI+SGEMKKDVPFLEEVVVMEGVGHFLQE
Sbjct: 320 FIVGDLDLTYNSFGAKQYIHSGEMKKDVPFLEEVVVMEGVGHFLQE 362

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BLAST of MS014636 vs. NCBI nr
Match: XP_024197743.1 (epoxide hydrolase A [Rosa chinensis] >PRQ39267.1 putative soluble epoxide hydrolase [Rosa chinensis])

HSP 1 Score: 517.3 bits (1331), Expect = 1.1e-142
Identity = 236/319 (73.98%), Postives = 268/319 (84.01%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGEGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLR 109
           ME I H TV VNGINMHVAEKG+GPV+LFIHGFPELWY+WRHQI  LAS GYRAVAPDLR
Sbjct: 1   MEGIEHRTVKVNGINMHVAEKGQGPVILFIHGFPELWYTWRHQIQALASLGYRAVAPDLR 60

Query: 110 GYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRI 169
           GYGD+DAP   S YT FH+VGDLVAL+DA+   +EKVFVV HDWGA+IAWY CL+RPDR+
Sbjct: 61  GYGDTDAPADPSSYTYFHVVGDLVALLDAVAADQEKVFVVGHDWGALIAWYFCLFRPDRV 120

Query: 170 RALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLT 229
           +ALVNMSVAFT R+P+RK +E+LRA+YGDDYY+CRFQEPGEIEAEFA+IG  RVM EFLT
Sbjct: 121 KALVNMSVAFTPRNPQRKTVETLRAVYGDDYYMCRFQEPGEIEAEFAQIGAARVMKEFLT 180

Query: 230 YHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNW 289
           Y  P PL L KGKGFGHP D PIPLP WLS  D+ YY S F+ KGFT  INYYRNLDRNW
Sbjct: 181 YRHPGPLFLSKGKGFGHPPDAPIPLPSWLSEDDVNYYASFFEKKGFTGGINYYRNLDRNW 240

Query: 290 ELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFL 349
           EL AP+TGAQ+KVPVKFIVGD DL YNS G K++I+ G +KK VPFL+EVVVMEGV HF+
Sbjct: 241 ELTAPWTGAQIKVPVKFIVGDQDLVYNSVGGKDFIHKGGLKKYVPFLQEVVVMEGVAHFI 300

Query: 350 QEEKPREISDHIYQFITKF 369
            EE+P EIS+HI+ FI KF
Sbjct: 301 NEERPTEISEHIHDFIKKF 319

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BLAST of MS014636 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.7e-55
Identity = 123/321 (38.32%), Postives = 173/321 (53.89%), Query Frame = 0


Query: 58  VSVNGINMHVAEKGE--GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSD 117
           V  NG+ + V E GE   PVV+  HGFPEL YSWRHQI  LA  GY  +APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 118 APPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNM 177
            P +   Y    +  DLV L+D  VGAE  V+ V HDWGA++ W   L   DR+ A+  +
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDD-VGAERAVW-VGHDWGAVVVWNAPLLHADRVAAVAAL 128

Query: 178 SVAFTRRSPKRKPIESLRALYGDD-YYICRFQEPGEIEAEFAKIGTERVMTEFLTYHTP- 237
           SV    R+ +  P ++ R+ +G++ +YI  FQEPG  +AE       R M   +    P 
Sbjct: 129 SVPALPRA-QVPPTQAFRSRFGENFFYILYFQEPGIADAEL-NGDPARTMRRMIGGLRPP 188

Query: 238 ------KPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDR 297
                   ++ P   GF   L  P  LP W+S +++ +Y  +F   GFT  +N+YRN DR
Sbjct: 189 GDQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDR 248

Query: 298 NWELNAPFTGAQVKVPVKFIVGDLD--LSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGV 357
           NWE  A   G  + VP  FI G  D  L++        + SG      P+ E  V+++G 
Sbjct: 249 NWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG------PYRE--VLIDGA 308

Query: 358 GHFLQEEKPREISDHIYQFIT 367
           GH+LQ+E+P E++  + +F+T
Sbjct: 309 GHWLQQERPGEVTAALLEFLT 317

[Close]

BLAST of MS014636 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.1e-48
Identity = 109/349 (31.23%), Postives = 183/349 (52.44%), Query Frame = 0


Query: 37  TETAKSKMEKESGME---------------SIRHSTVSVN-GINMHVAEKGEGPVVLFIH 96
           T+TA  ++EK +G++               ++ H  V +  G+ +H  E G GP V   H
Sbjct: 206 TDTALRELEKVTGVQLLQTPALPPTSCDPSALSHGYVLIKPGVRLHFVEMGSGPAVCLCH 265

Query: 97  GFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIV 156
           GFPE W+SWR+QI  LA  G+R +A D++GYG+S APP    Y+   +  D+V  ++ + 
Sbjct: 266 GFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIEEYSLEVLCKDMVTFLNKL- 325

Query: 157 GAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDY 216
           G  + VF + HDWG ++ W + L+ P+R+RA+ +++  F   +P   P+E ++A    DY
Sbjct: 326 GLSQAVF-IGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSNPNVSPMEIIKANPVFDY 385

Query: 217 YICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLS- 276
            +  FQEPG  EAE  +   +R    F   H    L   + +  G  L    P  P LS 
Sbjct: 386 QL-YFQEPGVAEAELEQ-NLDRTFKNFFRAHDETFLTTNRVRELG-GLFVGTPEEPSLSR 445

Query: 277 ---HQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYN 336
               +DIQ+Y  +F   GF  P+N+YRN++RNW+     +G ++ +P   +  + DL  +
Sbjct: 446 LVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKILIPALMVTAENDLVLH 505

Query: 337 SFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREISDHIYQFI 366
              +K       M+  +P L+   + +  GH+ Q +KP E++  + +++
Sbjct: 506 PKMSK------HMENWIPHLKRGHIKD-CGHWTQIDKPAELNRILIEWL 542

[Close]

BLAST of MS014636 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 6.7e-47
Identity = 109/349 (31.23%), Postives = 178/349 (51.00%), Query Frame = 0


Query: 37  TETAKSKMEKESGME---------------SIRHSTVSVN-GINMHVAEKGEGPVVLFIH 96
           T+TA  ++EK +G++                + H  V+V   + +H  E G GP V   H
Sbjct: 206 TDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCH 265

Query: 97  GFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIV 156
           GFPE WYSWR+QI  LA  GYR +A D++GYG+S APP    Y    +  ++V  +D + 
Sbjct: 266 GFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKL- 325

Query: 157 GAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDY 216
           G  + VF + HDWG ++ WY+ L+ P+R+RA+ +++  F   +P   P+ES++A    DY
Sbjct: 326 GLSQAVF-IGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDY 385

Query: 217 YICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPK----GKGFGHPLDTPIPLPP 276
            +  FQEPG  EAE  +    R             L + K    G  F +  + P  L  
Sbjct: 386 QL-YFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEP-SLSR 445

Query: 277 WLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYN 336
            ++ ++IQ+Y  +F   GF  P+N+YRN++RNW+      G ++ +P   +  + D    
Sbjct: 446 MVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDF--- 505

Query: 337 SFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREISDHIYQFI 366
                    S  M+  +P L+   + E  GH+ Q +KP E++  + +++
Sbjct: 506 ---VLVPQMSQHMEDWIPHLKRGHI-EDCGHWTQMDKPTEVNQILIKWL 542

[Close]

BLAST of MS014636 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-46
Identity = 106/346 (30.64%), Postives = 179/346 (51.73%), Query Frame = 0


Query: 25  SSPIRAGPKQNQTETAKSKMEKESGMESIRHSTVSVN-GINMHVAEKGEGPVVLFIHGFP 84
           +S +R   K   T+  ++ +        + H  V+V  GI +H  E G GP +   HGFP
Sbjct: 207 ASALRELEKVTGTQFPEAPLPVPCSPNDVSHGYVTVKPGIRLHFVEMGSGPAICLCHGFP 266

Query: 85  ELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAE 144
           E W+SWR+QI  LA  G+R +A D++GYGDS +PP    Y    +  ++V  ++ + G  
Sbjct: 267 ESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCEEMVTFLNKL-GIP 326

Query: 145 EKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYIC 204
           + VF + HDW  ++ W + L+ P+R+RA+ +++      +P+  P+E +R++   +Y + 
Sbjct: 327 QAVF-IGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQL- 386

Query: 205 RFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLS---- 264
            FQEPG  EAE  K  +    + F T      L + K    G  L    P  P +S    
Sbjct: 387 YFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATEMGGIL-VGTPEDPKVSKITT 446

Query: 265 HQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFG 324
            ++I+YY  +F   GF  P+N+YRN +RNW+ +    G ++ VP   +  + D+      
Sbjct: 447 EEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKILVPALMVTAEKDIVLRPEM 506

Query: 325 TKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREISDHIYQFI 366
           +K       M+  +PFL+   + E  GH+ Q EKP E++  + +++
Sbjct: 507 SK------NMENWIPFLKRGHI-EDCGHWTQIEKPAEVNQILIKWL 541

[Close]

BLAST of MS014636 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 9.7e-46
Identity = 106/346 (30.64%), Postives = 178/346 (51.45%), Query Frame = 0


Query: 25  SSPIRAGPKQNQTETAKSKMEKESGMESIRHSTVSVN-GINMHVAEKGEGPVVLFIHGFP 84
           +S +R   K   T+  ++ +        + H  V+V  GI +H  E G GP +   HGFP
Sbjct: 207 ASALRELEKVTGTQFPEAPLPVPCNPNDVSHGYVTVKPGIRLHFVEMGSGPALCLCHGFP 266

Query: 85  ELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAE 144
           E W+SWR+QI  LA  G+R +A D++GYGDS +PP    Y    +  ++V  +D + G  
Sbjct: 267 ESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCKEMVTFLDKL-GIP 326

Query: 145 EKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYIC 204
           + VF + HDW  ++ W + L+ P+R+RA+ +++  F    P   P++ +R++   +Y + 
Sbjct: 327 QAVF-IGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPDPDVSPMKVIRSIPVFNYQL- 386

Query: 205 RFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLS---- 264
            FQEPG  EAE  K  +    + F        + + K    G  L    P  P LS    
Sbjct: 387 YFQEPGVAEAELEKNMSRTFKSFFRASDETGFIAVHKATEIGGIL-VNTPEDPNLSKITT 446

Query: 265 HQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFG 324
            ++I++Y  +F   GF  P+N+YRN +RNW+ +    G ++ VP   +  + D+      
Sbjct: 447 EEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKILVPALMVTAEKDIVLRPEM 506

Query: 325 TKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREISDHIYQFI 366
           +K       M+K +PFL+   + E  GH+ Q EKP E++  + +++
Sbjct: 507 SK------NMEKWIPFLKRGHI-EDCGHWTQIEKPTEVNQILIKWL 541

[Close]

BLAST of MS014636 vs. ExPASy TrEMBL
Match: A0A1S3AXG8 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103483863 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.6e-162
Identity = 275/371 (74.12%), Postives = 309/371 (83.29%), Query Frame = 0


Query: 1   RIEIEPMCRFSWQFLRGLFNSPPLSSPIRAGPKQNQTETAKSKMEKESGMESIRHSTVSV 60
           ++  E M      FL  LFN   LS PI   P     +T   KME +  + SIRH T++V
Sbjct: 2   KVTFESMSTIFRHFLSKLFNFHMLSPPIIQSP---NPQTHSPKMENQMELSSIRHRTLNV 61

Query: 61  NGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRGYGDSDAPPS 120
           NGIN+H+AEKGE GP++LFIHGFPELWYSWR+QILDL+SRGYRAVAPDLRGYGDSD+PPS
Sbjct: 62  NGINLHIAEKGESGPLILFIHGFPELWYSWRYQILDLSSRGYRAVAPDLRGYGDSDSPPS 121

Query: 121 QSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIRALVNMSVAF 180
            + YT FHIVGDL+ALIDAIVG EEKVFVV HDWGA+IAW LC+YRPDR++ALVN SV F
Sbjct: 122 VNDYTCFHIVGDLIALIDAIVGVEEKVFVVGHDWGAMIAWNLCMYRPDRVKALVNTSVTF 181

Query: 181 TRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLTYHTPKPLMLP 240
            RRSPKRKPI +L+ALYGDDYYICRFQEPGEIEAEFA+IGTER+MTE L+Y  PKPLM+P
Sbjct: 182 NRRSPKRKPIPALKALYGDDYYICRFQEPGEIEAEFAEIGTERIMTEILSYRIPKPLMMP 241

Query: 241 KGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNWELNAPFTG 300
           KG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PINYYRNLDRNWELNAPFTG
Sbjct: 242 KGRGKGKDHPLDTPISLPPWLTKQDMDYYVSKFDKNGFTGPINYYRNLDRNWELNAPFTG 301

Query: 301 AQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFLQEEKPREI 360
           AQVKVP KFIVGD DL+YNSFG K+YI SGEMKKDVPFLEEVVVMEGVGHFLQEEKP EI
Sbjct: 302 AQVKVPTKFIVGDQDLTYNSFGAKQYIESGEMKKDVPFLEEVVVMEGVGHFLQEEKPHEI 361

Query: 361 SDHIYQFITKF 369
           S+HIY+FI  +
Sbjct: 362 SNHIYEFIKNY 369

[Close]

BLAST of MS014636 vs. ExPASy TrEMBL
Match: A0A5A7U167 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001200 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.8e-157
Identity = 261/328 (79.57%), Postives = 292/328 (89.02%), Query Frame = 0


Query: 44  MEKESGMESIRHSTVSVNGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYR 103
           ME +  + SIRH T++VNGIN+H+AEKGE GP++LFIHGFPELWYSWR+QILDL+SRGYR
Sbjct: 1   MENQMELSSIRHRTLNVNGINLHIAEKGESGPLILFIHGFPELWYSWRYQILDLSSRGYR 60

Query: 104 AVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLC 163
           AVAPDLRGYGDSD+PPS + YT FHIVGDL+ALIDAIVG EEKVFVV HDWGA+IAW LC
Sbjct: 61  AVAPDLRGYGDSDSPPSVNDYTCFHIVGDLIALIDAIVGVEEKVFVVGHDWGAMIAWNLC 120

Query: 164 LYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTER 223
           +YRPDR++ALVN SV F RRSPKRKPI +L+ALYGDDYYICRFQEPGEIEAEFA+IGTER
Sbjct: 121 MYRPDRVKALVNTSVTFNRRSPKRKPIPALKALYGDDYYICRFQEPGEIEAEFAEIGTER 180

Query: 224 VMTEFLTYHTPKPLMLPKGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPIN 283
           +MTE L+Y  PKPLM+PKG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PIN
Sbjct: 181 IMTEILSYRIPKPLMMPKGRGKGKDHPLDTPISLPPWLTKQDMDYYVSKFDKNGFTGPIN 240

Query: 284 YYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVV 343
           YYRNLDRNWELNAPFTGAQVKVP KFIVGD DL+YNSFG K+YI SGEMKKDVPFLEEVV
Sbjct: 241 YYRNLDRNWELNAPFTGAQVKVPTKFIVGDQDLTYNSFGAKQYIESGEMKKDVPFLEEVV 300

Query: 344 VMEGVGHFLQEEKPREISDHIYQFITKF 369
           VMEGVGHFLQEEKP EIS+HIY+FI  +
Sbjct: 301 VMEGVGHFLQEEKPHEISNHIYEFIKNY 328

[Close]

BLAST of MS014636 vs. ExPASy TrEMBL
Match: A0A0A0L5A5 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G164490 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.8e-157
Identity = 259/322 (80.43%), Postives = 291/322 (90.37%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGE-GPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDL 109
           + SIRH+T+++NGIN+H+AEKGE GP++LFIHGFPELWYSWRHQILDL+SRGYRAVAPDL
Sbjct: 3   LSSIRHTTLNLNGINLHIAEKGESGPLILFIHGFPELWYSWRHQILDLSSRGYRAVAPDL 62

Query: 110 RGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDR 169
           RGYGDSD+PPS + YT FHIVGDL+ALIDA+VG EEKVFVV HDWGA+IAW LC+YRPDR
Sbjct: 63  RGYGDSDSPPSVNDYTCFHIVGDLIALIDALVGVEEKVFVVGHDWGAVIAWNLCMYRPDR 122

Query: 170 IRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFL 229
           ++ALVN SV F RRSPKRKPIESL+ALYGDDYYICRFQEPGEIEAEFA+IGTER+MTE L
Sbjct: 123 VKALVNTSVTFNRRSPKRKPIESLKALYGDDYYICRFQEPGEIEAEFAEIGTERIMTEIL 182

Query: 230 TYHTPKPLMLPKGKGFG--HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLD 289
           +Y TPKPLM+PKG+G G  HPLDTPI LPPWL+ QD+ YY SKFD  GFT PINYYRNLD
Sbjct: 183 SYRTPKPLMMPKGRGKGKDHPLDTPISLPPWLAKQDMDYYVSKFDKNGFTGPINYYRNLD 242

Query: 290 RNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVG 349
           RNWELNA FTGAQVKVP KFIVGD DL+Y+SFG K+YI SGEMKKDVPFLEEVVVMEGVG
Sbjct: 243 RNWELNASFTGAQVKVPTKFIVGDQDLTYHSFGAKQYIQSGEMKKDVPFLEEVVVMEGVG 302

Query: 350 HFLQEEKPREISDHIYQFITKF 369
           HFLQEEKP EIS+HIY+FI  +
Sbjct: 303 HFLQEEKPHEISNHIYEFIKNY 324

[Close]

BLAST of MS014636 vs. ExPASy TrEMBL
Match: A0A2P6QYJ7 (Putative soluble epoxide hydrolase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0423281 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 5.2e-143
Identity = 236/319 (73.98%), Postives = 268/319 (84.01%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGEGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLR 109
           ME I H TV VNGINMHVAEKG+GPV+LFIHGFPELWY+WRHQI  LAS GYRAVAPDLR
Sbjct: 1   MEGIEHRTVKVNGINMHVAEKGQGPVILFIHGFPELWYTWRHQIQALASLGYRAVAPDLR 60

Query: 110 GYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRI 169
           GYGD+DAP   S YT FH+VGDLVAL+DA+   +EKVFVV HDWGA+IAWY CL+RPDR+
Sbjct: 61  GYGDTDAPADPSSYTYFHVVGDLVALLDAVAADQEKVFVVGHDWGALIAWYFCLFRPDRV 120

Query: 170 RALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLT 229
           +ALVNMSVAFT R+P+RK +E+LRA+YGDDYY+CRFQEPGEIEAEFA+IG  RVM EFLT
Sbjct: 121 KALVNMSVAFTPRNPQRKTVETLRAVYGDDYYMCRFQEPGEIEAEFAQIGAARVMKEFLT 180

Query: 230 YHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNW 289
           Y  P PL L KGKGFGHP D PIPLP WLS  D+ YY S F+ KGFT  INYYRNLDRNW
Sbjct: 181 YRHPGPLFLSKGKGFGHPPDAPIPLPSWLSEDDVNYYASFFEKKGFTGGINYYRNLDRNW 240

Query: 290 ELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFL 349
           EL AP+TGAQ+KVPVKFIVGD DL YNS G K++I+ G +KK VPFL+EVVVMEGV HF+
Sbjct: 241 ELTAPWTGAQIKVPVKFIVGDQDLVYNSVGGKDFIHKGGLKKYVPFLQEVVVMEGVAHFI 300

Query: 350 QEEKPREISDHIYQFITKF 369
            EE+P EIS+HI+ FI KF
Sbjct: 301 NEERPTEISEHIHDFIKKF 319

[Close]

BLAST of MS014636 vs. ExPASy TrEMBL
Match: A0A7N2LXE1 (AB hydrolase-1 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 2.0e-142
Identity = 229/319 (71.79%), Postives = 271/319 (84.95%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGEGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLR 109
           ME I H +V+VNGINMH+AEKG+GPV+LFIHGFPELWYSWRHQI+ LAS+GYRAVAPDLR
Sbjct: 1   MEGIEHRSVNVNGINMHIAEKGQGPVILFIHGFPELWYSWRHQIIALASQGYRAVAPDLR 60

Query: 110 GYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRI 169
           GYGD+DAPPS + YT FH+VGDL+AL+DA+   ++KVFVV HDWGA+IAWYLCL+RPDR+
Sbjct: 61  GYGDTDAPPSITSYTCFHVVGDLIALLDAVAPDQDKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 170 RALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLT 229
           +ALVNMSV  + R+PKRKP+ SLRA+YG+DYY+CRFQEPGEIEAEFA++GT  V+ EFLT
Sbjct: 121 KALVNMSVVLSPRNPKRKPLVSLRAIYGNDYYMCRFQEPGEIEAEFAQLGTATVLKEFLT 180

Query: 230 YHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNW 289
           Y  P PL LPKGKGFGHP   PI LP WLS +DI YY SKF+  GFT  +NYYR+LD NW
Sbjct: 181 YRNPGPLFLPKGKGFGHPPGAPIVLPSWLSEEDINYYASKFEKAGFTGGVNYYRDLDLNW 240

Query: 290 ELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFL 349
           EL AP+TGAQ+KVPVKFIVGDLDL+YNS GTK+YI+ G  K+DVP LEEV+V+E VGHF+
Sbjct: 241 ELTAPWTGAQIKVPVKFIVGDLDLTYNSLGTKDYIHKGGFKRDVPLLEEVIVLEDVGHFI 300

Query: 350 QEEKPREISDHIYQFITKF 369
            EEKP EI+ HIY F  KF
Sbjct: 301 HEEKPDEINKHIYDFFQKF 319

[Close]

BLAST of MS014636 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 449.1 bits (1154), Expect = 3.4e-126
Identity = 200/319 (62.70%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKGEGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLR 109
           ME I H+T+S NGINMHVA  G GPV+LF+HGFP+LWYSWRHQ++  A+ GYRA+APDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 110 GYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRI 169
           GYGDSDAPPS+  YT  HIVGDLV L+D++    ++VF+V HDWGAI+AW+LC+ RPDR+
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSL--GVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 170 RALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFLT 229
            ALVN SV F  R+P  KP+++ RAL+GDDYYICRFQEPGEIE +FA++ T++++T F T
Sbjct: 121 NALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFT 180

Query: 230 YHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRNW 289
              P+P  +PK  GF   L  P  LP WL+ QD+++Y  KF  KGFT  +NYYR L+ +W
Sbjct: 181 SRNPRPPCIPKSVGF-RGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSW 240

Query: 290 ELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHFL 349
           EL AP+TG Q+KVPVKFIVGDLD++YN  GTKEYI+ G +KK VPFL+EVVVMEGVGHFL
Sbjct: 241 ELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFL 300

Query: 350 QEEKPREISDHIYQFITKF 369
            +EKP E++DHIY F  KF
Sbjct: 301 HQEKPDEVTDHIYGFFKKF 316

[Close]

BLAST of MS014636 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.0e-122
Identity = 194/333 (58.26%), Postives = 254/333 (76.28%), Query Frame = 0


Query: 41  KSKMEKESGMESIRHSTVSVNGINMHVAEK-----GEGPVVLFIHGFPELWYSWRHQILD 100
           ++K  ++S ++ + H T+ VNGINMHVAEK     GE P++LF+HGFPELWY+WRHQ++ 
Sbjct: 42  QTKRPEKSRLDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVA 101

Query: 101 LASRGYRAVAPDLRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGA 160
           L+S GYR +APDLRGYGD++AP     YT  ++ GD+VALIDA+ G ++ V VV HDWGA
Sbjct: 102 LSSLGYRTIAPDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGA 161

Query: 161 IIAWYLCLYRPDRIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEF 220
           +IAW LC YRP++++ALVNMSV F+ R+P R P+ +LR ++GDDYY+CRFQ+ GEIE EF
Sbjct: 162 MIAWQLCQYRPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEF 221

Query: 221 AKIGTERVMTEFLTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGF 280
            K+GTE V+ EFLTY TP PL LPK K F    +    LP WL+ +D+ YY +K++ KGF
Sbjct: 222 KKLGTENVLKEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGF 281

Query: 281 TAPINYYRNLDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPF 340
           T PINYYRN+DRNWEL AP+TGA+++VPVKFI+GD DL+YN  G KEYIN G  K+DVP 
Sbjct: 282 TGPINYYRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPL 341

Query: 341 LEEVVVMEGVGHFLQEEKPREISDHIYQFITKF 369
           L+E VV++G+GHFL EE P  I+ HI+ F  KF
Sbjct: 342 LDETVVLKGLGHFLHEENPDVINQHIHNFFHKF 374

[Close]

BLAST of MS014636 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 419.5 bits (1077), Expect = 2.8e-117
Identity = 194/321 (60.44%), Postives = 246/321 (76.64%), Query Frame = 0


Query: 53  IRHSTVSVNGINMHVAEKG--EGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRG 112
           + H  V  NGI++HVA +G  +GP+VL +HGFPELWYSWRHQI  LA+RGYRAVAPDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 113 YGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAE-EKVFVVAHDWGAIIAWYLCLYRPDRI 172
           YGDSDAP   S YT F+IVGDL+A+I A+  +E EKVFVV HDWGA+IAWYLCL+RPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 173 RALVNMSVAFTRR--SPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEF 232
           +ALVN+SV F+ R   P  KP++ +RA YGDDYYICRFQE G++EAE A++GTERVM   
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 233 LTYHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDR 292
           LTY TP P+++PK K F       IPLP WL+ +D+ Y+ SKF+ KGF+ P+NYYRN +R
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 293 NWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGH 352
           N EL  P+ G++++VP KF++G+LDL Y   G KEYI+  + K+DVP LEE VVMEGV H
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 353 FLQEEKPREISDHIYQFITKF 369
           F+ +EKP+EI   I  FI+KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

[Close]

BLAST of MS014636 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 5.0e-114
Identity = 187/320 (58.44%), Postives = 241/320 (75.31%), Query Frame = 0


Query: 53  IRHSTVSVNGINMHVAEKG--EGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPDLRG 112
           + H  V  NGI++HVA +G  +G +VL +HGFPELWYSWRHQI  LA+RGYRAVAPDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 113 YGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPDRIR 172
           YGDSDAP   S +T F+IVGDLVA+I  ++  ++KVFVV HDWGA+IAWYLCL+RPD+++
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 173 ALVNMSV--AFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEFL 232
           ALVN+SV  +F    P  KP++ +RA+YG+DYY+CRFQE G+IEAE A++GTERVM   L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 233 TYHTPKPLMLPKGKGFGHPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRNLDRN 292
           TY TP PL++PK K F       IPLP WL+ +D+ Y+ SKF  KGF  P+NYYRN +RN
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 293 WELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEGVGHF 352
            EL  P+ G++++VP KF++G+LDL Y   G KEYI+  + K+DVP +EE VVMEGV HF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 353 LQEEKPREISDHIYQFITKF 369
           L +EKP+EI   I  FI+ F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

[Close]

BLAST of MS014636 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 182/323 (56.35%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 50  MESIRHSTVSVNGINMHVAEKG--EGPVVLFIHGFPELWYSWRHQILDLASRGYRAVAPD 109
           ME I H  VSVNGI MH+AEKG  EGPVVL +HGFP+LWY+WRHQI  L+S GYRAVAPD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 110 LRGYGDSDAPPSQSGYTSFHIVGDLVALIDAIVGAEEKVFVVAHDWGAIIAWYLCLYRPD 169
           LRGYGDSD+P S S YT  ++VGDLVAL+D++ G +EKVF+V HDWGAII W+LCL+RP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 170 RIRALVNMSVAFTRRSPKRKPIESLRALYGDDYYICRFQEPGEIEAEFAKIGTERVMTEF 229
           +I   V +SV +  R+PK KP++  +A++GDDYYICRFQEPG+IE E A       +   
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 230 LTYHTPKPLMLPKGKGFG---HPLDTPIPLPPWLSHQDIQYYTSKFDTKGFTAPINYYRN 289
            T  T  P +LPK   FG   +P    I LP W S +D+ +Y SKF+  GFT  +NYYR 
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 290 LDRNWELNAPFTGAQVKVPVKFIVGDLDLSYNSFGTKEYINSGEMKKDVPFLEEVVVMEG 349
           +D NWEL AP+TGA+++VPVKF+ GD D+ Y + G KEYI+ G    DVP L+E+VV+E 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 350 VGHFLQEEKPREISDHIYQFITK 368
            GHF+ +EKP+E++ HI  F TK
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

The following BLAST results are available for this feature:

BLAST of MS014636 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438903.1	1.8e-161	74.12	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo]	[more]
XP_004134492.1	5.9e-157	80.43	uncharacterized protein LOC101212443 [Cucumis sativus] >KGN57130.1 hypothetical ...	[more]
KAA0049512.1	5.9e-157	79.57	bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK16192.1 bif...	[more]
XP_038894580.1	1.4e-155	78.03	epoxide hydrolase A-like [Benincasa hispida]	[more]
XP_024197743.1	1.1e-142	73.98	epoxide hydrolase A [Rosa chinensis] >PRQ39267.1 putative soluble epoxide hydrol...	[more]

BLAST of MS014636 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I6YGS0	6.7e-55	38.32	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]
Q6Q2C2	6.1e-48	31.23	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]
P34913	6.7e-47	31.23	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
P80299	1.5e-46	30.64	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=...	[more]
P34914	9.7e-46	30.64	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]

BLAST of MS014636 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AXG8	8.6e-162	74.12	bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103483863 PE...	[more]
A0A5A7U167	2.8e-157	79.57	Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0L5A5	2.8e-157	80.43	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G164...	[more]
A0A2P6QYJ7	5.2e-143	73.98	Putative soluble epoxide hydrolase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0...	[more]
A0A7N2LXE1	2.0e-142	71.79	AB hydrolase-1 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of MS014636 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02340.1	3.4e-126	62.70	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	1.0e-122	58.26	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	2.8e-117	60.44	soluble epoxide hydrolase	[more]
AT2G26750.1	5.0e-114	58.44	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05600.1	1.5e-110	56.35	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 342..364 score: 42.49 coord: 100..115 score: 63.92 coord: 148..161 score: 54.87 coord: 302..318 score: 36.63 coord: 162..175 score: 38.31 coord: 80..98 score: 60.77
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 74..187 e-value: 5.3E-21 score: 75.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 50..368 e-value: 3.2E-108 score: 364.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 44..367
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 25..51
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 50..368
None	No IPR available	PANTHER	PTHR43329:SF58	OS05G0273800 PROTEIN	coord: 50..368

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014636.1	MS014636.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity