Homology
BLAST of MS014628 vs. NCBI nr
Match:
XP_022137811.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Momordica charantia])
HSP 1 Score: 2572.7 bits (6667), Expect = 0.0e+00
Identity = 1316/1343 (97.99%), Postives = 1317/1343 (98.06%), Query Frame = 0
Query: 3 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ 62
MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ
Sbjct: 1 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ 60
Query: 63 RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG 122
RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG
Sbjct: 61 RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG 120
Query: 123 TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT 182
TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT
Sbjct: 121 TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT 180
Query: 183 SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNC 242
SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSN
Sbjct: 181 SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNS 240
Query: 243 MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN 302
MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN
Sbjct: 241 MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN 300
Query: 303 SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN 362
SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN
Sbjct: 301 SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN 360
Query: 363 YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK 422
YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK
Sbjct: 361 YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK 420
Query: 423 KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN 482
KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN
Sbjct: 421 KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN 480
Query: 483 QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT 542
QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT
Sbjct: 481 QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT 540
Query: 543 GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGLQLCDLA 602
GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGL+LCDLA
Sbjct: 541 GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGLKLCDLA 600
Query: 603 TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM 662
TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM
Sbjct: 601 TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM 660
Query: 663 IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR 722
IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR
Sbjct: 661 IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR 720
Query: 723 GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKAVPLTQFSFI 782
GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVP
Sbjct: 721 GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVP---------------------- 780
Query: 783 LFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK 842
RSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK
Sbjct: 781 --RSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK 840
Query: 843 EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI 902
EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI
Sbjct: 841 EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI 900
Query: 903 KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG 962
KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG
Sbjct: 901 KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG 960
Query: 963 CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG 1022
CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG
Sbjct: 961 CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG 1020
Query: 1023 VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL 1082
VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL
Sbjct: 1021 VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL 1080
Query: 1083 FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ 1142
FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ
Sbjct: 1081 FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ 1140
Query: 1143 GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA 1202
GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA
Sbjct: 1141 GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA 1200
Query: 1203 RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL 1262
RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL
Sbjct: 1201 RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL 1260
Query: 1263 FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG 1322
FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG
Sbjct: 1261 FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG 1319
Query: 1323 SRITCLLTANDMVLCGTETGKIK 1346
SRITCLLTANDMVLCGTETGK K
Sbjct: 1321 SRITCLLTANDMVLCGTETGKTK 1319
BLAST of MS014628 vs. NCBI nr
Match:
XP_038894856.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Benincasa hispida])
HSP 1 Score: 2216.8 bits (5743), Expect = 0.0e+00
Identity = 1141/1352 (84.39%), Postives = 1210/1352 (89.50%), Query Frame = 0
Query: 2 CM--APASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CM A +SS TQ PPSVCENERLDLNSIR LVVSINQYI EFLSNAE R A+ LRCTSKL
Sbjct: 5 CMLTASSSSPTQCPPSVCENERLDLNSIRGLVVSINQYIQEFLSNAEVRTAVKLRCTSKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RNQRHGF EFLEQSIISNLY GIE +EDAVQTS SE RATRLQTAEQMLQVPALLDE GE
Sbjct: 65 RNQRHGFLEFLEQSIISNLYGGIEYIEDAVQTSTSEARATRLQTAEQMLQVPALLDEDGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG NRYLVCCSYFYLS+VKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCY+LLLS
Sbjct: 125 TSGMANRYLVCCSYFYLSLVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYNLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
F+T SRQEN ++M S+S VEFGEGDYGESTIRQVARKYKDWLMYYQVM YGET QW+Q G
Sbjct: 185 FSTCSRQENCKSMGSNSFVEFGEGDYGESTIRQVARKYKDWLMYYQVMSYGETHQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S ML E+GSHS+ EAS+ATDCGFP PTL HYD + PLD DV QDK KASQD
Sbjct: 245 SRSMLSSEDGSHSLHGTFSRIEASEATDCGFPRPTLSHYDILPPLDYIDVFQDKRKASQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
F CED NS KKLG IPE QF++RG WRDSSTK I DLLKDSQPGSPTSLFSSMN SES
Sbjct: 305 FLQCEDTANSPKKLGFIPERQFSERGFWRDSSTKCIGDLLKDSQPGSPTSLFSSMNNSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD+E GMN TNHSKR+ RAD+PE F QKL+YAC+ +D EQSLISLSSASLS V+EQY K
Sbjct: 365 DSDLEAGMNDTNHSKRTARADMPENFYQKLQYACSNTDGEQSLISLSSASLSRVKEQYNK 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K + +K RS++QKNLEPQ+ QNCLEESEP ELS+ K Q+F SA+P LG
Sbjct: 425 SNMMKSISNKFNGYKSRSLKQKNLEPQVFQNCLEESEPKELSVDPCKLQSFDSALPLSLG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGSA Q+S Q K QLYH+ S RDSKSEILGL+EKAISRLCFSEGLG+Y++E AVEVST
Sbjct: 485 QGSACQISKQISVKGQLYHANSRRDSKSEILGLVEKAISRLCFSEGLGDYNDECAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+ NVHEAAILIYLISPSPREIKSL+LLPVLVEIICTSKCYNAWSPSL+
Sbjct: 605 KGLQLCDLATALKHNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNAWSPSLV 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGL SLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLASLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECRGYISKFI VA FLCLLQSDKKEAVHIALQVFNEILRVP
Sbjct: 725 CMQLDGECRGYISKFISVAPFLCLLQSDKKEAVHIALQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEGKNDIIHILMLCVNHL+TEYQL AAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGKNDIIHILMLCVNHLETEYQLWAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
NCSTTSLLKEEAVQVLLRSVACEE SAMQ LSASILS +GGTFSWTGEPYTVAWLL+KVG
Sbjct: 845 NCSTTSLLKEEAVQVLLRSVACEETSAMQLLSASILSTIGGTFSWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCSL+ARNII IGE VFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSLMARNIIYIGESVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
CLT IAWLGCEIAKSPSS R SACEILL GIELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 CLTAIAWLGCEIAKSPSSIRCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
QKL FSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QKLTRFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQHLGAYGQ+IFAITHGHGLKVIDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHLGAYGQMIFAITHGHGLKVIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWM+MH KAINSLAVYKDWLFS
Sbjct: 1205 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMLMHQKAINSLAVYKDWLFS 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASSMVQGSLFQNWRRH+KPE+ I+TGKGDIV AMS VEDFVY+I KSS NSIQIWLRK Q
Sbjct: 1265 ASSMVQGSLFQNWRRHEKPEINIITGKGDIVQAMSVVEDFVYIICKSSVNSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
HKVGR++AGS+ITCLLTANDMVLCGTETGKIK
Sbjct: 1325 HKVGRVTAGSKITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. NCBI nr
Match:
XP_023526729.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo] >XP_023526731.1 putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo] >XP_023526732.1 putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2206.8 bits (5717), Expect = 0.0e+00
Identity = 1134/1352 (83.88%), Postives = 1199/1352 (88.68%), Query Frame = 0
Query: 2 CMAPASSR--TQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CMA ASS TQ PP VCEN RLDLNSIR LVVSINQYIHEFLSNAEAR A+ LRC SKL
Sbjct: 5 CMATASSSSPTQCPPGVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRCISKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RN +H +FEFLEQSIISNLYWG+EN+EDAVQTS SE RAT+LQTAEQMLQVPALLDEHGE
Sbjct: 65 RNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSSSEVRATKLQTAEQMLQVPALLDEHGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG +NRYLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLS
Sbjct: 125 TSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
FAT SRQEN +MRSDS VEFGEGD+GEST+RQVARKYKDWLMYYQVM YGET+QW+Q G
Sbjct: 185 FATCSRQENCTSMRSDSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETRQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S+ M E+GSHS+ E SKA DCG PT+ HYD I PLD DV QDKT SQD
Sbjct: 245 SSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTNTSQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
P CE+ GNS K LGLIPE Q ND G WRDSSTK+I DLLKDS GSPTSLFSSMN SES
Sbjct: 305 VPRCEELGNSGKNLGLIPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMNDSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD E GMNYTNHSKRS + D+PE F QKLRYA +KSD EQSLISL+SASLS VQE YI+
Sbjct: 365 DSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQEHYIE 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K +D+KL S EQK+LEPQILQNC E+SEP EL + K QTF SA+P LG
Sbjct: 425 ANMMKSISNKFNDYKLCSEEQKDLEPQILQNCFEDSEPKELLVNPCKLQTFDSALPLALG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGS Q+S QN AK QLYH+ S +DSKSEILGL+EKAISRLCFSEGLGNYD+EYAVEVST
Sbjct: 485 QGSTCQISKQNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEII TS+CYN WSP LM
Sbjct: 605 KGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWSPPLM 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECR YISKFI VA FLCLLQSDKKEAVHI LQVFNEILRVP
Sbjct: 725 CMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEG NDIIHILMLCVNHLQTEYQLLAAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
N STTS LKEEAV VLLRSVACEE SAMQ LSASILS LGGTF+WTGEPYTVAWLL+KVG
Sbjct: 845 NGSTTSFLKEEAVHVLLRSVACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCSL+ARNIICIGEPVFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
CLTTIAWLGCEIAKSPSS R SACEILL IELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 CLTTIAWLGCEIAKSPSSIRCSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
QKL NFSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QKLTNFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQHLGAYGQIIFAITHGHGLKVIDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKC+KVVQ RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLF
Sbjct: 1205 LKCMKVVQGRVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFC 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASS+VQGSLFQNWRRH+KP+M IVTGKGD+V AMS VEDFVY+I KSS +SIQIWLRK Q
Sbjct: 1265 ASSIVQGSLFQNWRRHEKPKMNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
HKVGR+SA SRITCLLTANDMVLCGTETGKIK
Sbjct: 1325 HKVGRVSASSRITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. NCBI nr
Match:
KAG6582207.1 (putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2197.5 bits (5693), Expect = 0.0e+00
Identity = 1129/1352 (83.51%), Postives = 1197/1352 (88.54%), Query Frame = 0
Query: 2 CMAP--ASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CMA +SS TQ PP VCEN RLDLNSIR LVVSINQYIHEFLSNAEAR A+ LRC SKL
Sbjct: 5 CMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRCISKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RN +H +FEFLEQSIISNLYWG+EN+EDAVQTS SE RATRLQTAEQMLQVPALLDEHGE
Sbjct: 65 RNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATRLQTAEQMLQVPALLDEHGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG +NRYLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLS
Sbjct: 125 TSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
FAT SRQEN +MRS+S VEFGEGD+GEST+RQVARKYKDWLMYYQVM YGET QW+Q G
Sbjct: 185 FATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S+ M E+GSHS+ E SKA DCG PT+ HYD I PLD DV QDKT SQD
Sbjct: 245 SSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTNTSQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
P CE+ GNS+K LGL+PE Q ND G WRDSSTK+I DLLKDS GSPTSLFSSMN SES
Sbjct: 305 VPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMNDSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD E GMNYTNHSKRS + D+PE F QKLRYA +KSD EQSLISL+SASLS VQE YI+
Sbjct: 365 DSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQEHYIE 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K +D+KL S EQK+LEPQILQNCLE+SEP EL + K QTF SA+P LG
Sbjct: 425 ANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALPLSLG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGS Q+S +N AK QLYH+ S +DSKSEILGL+EKAISRLCFSEGLGNYD+EYAVEVST
Sbjct: 485 QGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEII TS+CYN WSP M
Sbjct: 605 KGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWSPPFM 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECR YISKFI VA FLCLLQSDKKEAVHI LQVFNEILRVP
Sbjct: 725 CMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEG NDIIHILMLCVNHLQTEYQLLAAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
N STTS LKEEAV VLLRSVACEE SAMQ LSASILS LGGTF+WTGEPYTVAWLL+KVG
Sbjct: 845 NGSTTSFLKEEAVHVLLRSVACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCSL+ARNIICIGEPVFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
LTTIAWLGCEIAKSPSS R SACEILL IELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 SLTTIAWLGCEIAKSPSSIRCSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
Q L NFSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QNLTNFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQHLGAYGQIIFAITHGHGLKVIDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKC+KVVQ RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLF
Sbjct: 1205 LKCMKVVQGRVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFC 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASS+VQGSLFQNWRRH+KP+M IVTGKGD+V AMS VEDFVY+I KSS +SIQIWLRK Q
Sbjct: 1265 ASSIVQGSLFQNWRRHEKPKMNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
HKVGR+SA SRITCLLTANDMVLCGTETGKIK
Sbjct: 1325 HKVGRVSASSRITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. NCBI nr
Match:
KAG7018606.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2196.4 bits (5690), Expect = 0.0e+00
Identity = 1128/1352 (83.43%), Postives = 1197/1352 (88.54%), Query Frame = 0
Query: 2 CMAP--ASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CMA +SS TQ PP VCEN RLDLNSIR LVVSINQYIHEFLSNAEAR A+ LRC SKL
Sbjct: 5 CMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRCISKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RN +H +FEFLEQSIISNLYWG+EN+EDAVQTS SE RAT+LQTAEQMLQVPALLDEHGE
Sbjct: 65 RNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATKLQTAEQMLQVPALLDEHGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG +NRYLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLS
Sbjct: 125 TSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
FAT SRQEN +MRS+S VEFGEGD+GEST+RQVARKYKDWLMYYQVM YGET QW+Q G
Sbjct: 185 FATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S+ M E+GSHS+ E SKA DCG PT+ HYD I PLD DV QDKT SQD
Sbjct: 245 SSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTNTSQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
P CE+ GNS+K LGL+PE Q ND G WRDSSTK+I DLLKDS GSPTSLFSSMN SES
Sbjct: 305 VPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMNDSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD E GMNYTNHSKRS + D+PE F QKLRYA +KSD EQSLISL+SASLS VQE YI+
Sbjct: 365 DSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQEHYIE 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K +D+KL S EQK+LEPQILQNCLE+SEP EL + K QTF SA+P LG
Sbjct: 425 ANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALPLSLG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGS Q+S +N AK QLYH+ S +DSKSEILGL+EKAISRLCFSEGLGNYD+EYAVEVST
Sbjct: 485 QGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEII TS+CYN WSP M
Sbjct: 605 KGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWSPPFM 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECR YISKFI VA FLCLLQSDKKEAVHI LQVFNEILRVP
Sbjct: 725 CMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEG NDIIHILMLCVNHLQTEYQLLAAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
N STTS LKEEAV VLLRSVACEE SAMQ LSASILS LGGTF+WTGEPYTVAWLL+KVG
Sbjct: 845 NGSTTSFLKEEAVHVLLRSVACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCSL+ARNIICIGEPVFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
LTTIAWLGCEIAKSPSS R SACEILL IELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 SLTTIAWLGCEIAKSPSSIRCSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
Q L NFSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QNLTNFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQHLGAYGQIIFAITHGHGLKVIDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKC+KVVQ RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLF
Sbjct: 1205 LKCMKVVQGRVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFC 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASS+VQGSLFQNWRRH+KP+M IVTGKGD+V AMS VEDFVY+I KSS +SIQIWLRK Q
Sbjct: 1265 ASSIVQGSLFQNWRRHEKPKMNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
HKVGR+SA SRITCLLTANDMVLCGTETGKIK
Sbjct: 1325 HKVGRVSASSRITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. ExPASy Swiss-Prot
Match:
C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)
HSP 1 Score: 288.5 bits (737), Expect = 3.9e-76
Identity = 347/1518 (22.86%), Postives = 642/1518 (42.29%), Query Frame = 0
Query: 27 IRALVVSINQYIHEFLSNAEARIALTLRCTSKL-----RNQRHGFFEFLEQSIISNLYWG 86
+R L +++ +I + L N E R +C +L + E+ +Q++++NL WG
Sbjct: 15 VRFLTTTVDSFIQDRLINKEQRTQHKEQCAERLAAEDGSGDKDTEVEYSDQAVLANLDWG 74
Query: 87 IENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSGTENRYLVCCSYFYLSVVKK 146
IE +E+A+ T E + RL AE+MLQV A+L+ + +G N YL ++ LS + K
Sbjct: 75 IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKIAGVPNSYLSAWAHLNLSYLWK 134
Query: 147 LQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFATS-----SRQENG---ETMR 206
L+ + H L+ +V P +FA SL L +S S + + E +
Sbjct: 135 LRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVGWYSEERHRLMMEVIP 194
Query: 207 SDSSVEFG---EGDYGESTIRQVARKYKDWLMYYQVMLYGE-----TQQWKQDGSNCMLP 266
+ + F E + ES + + + L + LYGE T+ + + ++CM
Sbjct: 195 DSADLSFTADFEQFFNESLVLTMRPHQLEKLQKLE-QLYGESLDENTKLYAKYYNDCM-- 254
Query: 267 PENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGNSQK 326
+ S S +A P T +H L RT I D K P + +
Sbjct: 255 -NSDSSSSKKAVPMLPIAEPPMT-----PLHELSRT--IPDFVKFGPILPKSAGFSLAPR 314
Query: 327 KLGLIPE----------LQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFS-SMNGSESD 386
++ E L+ W T ++++++ S + L + S++ + +
Sbjct: 315 SKDVLNETIRENVTSSNLKEEKLSIWGAKDT-----IIEENEDDSDSELENESVDSDDKN 374
Query: 387 SDIEEGMNYTNHSKRSTRADL---------PEKFS--QKLRYACTKSDPEQSLISLSSAS 446
+ GM + T+ DL P+ FS R A S P + S +
Sbjct: 375 NIFSPGMKMMKYEGVETKVDLSCQRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSK 434
Query: 447 LSTVQEQYIKVSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSK--- 506
+ I+ S D + +I + E +L+N +++ LS+
Sbjct: 435 FLRLSSSRIREPTISDSLTSSPDISIDNISNADNEVMVLKNIQRKNDNQTLSMNHENENS 494
Query: 507 --------------FQTFGS---------------------------------------- 566
+Q+F S
Sbjct: 495 LILNGSSLCESDDGYQSFNSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYER 554
Query: 567 -AIPSFLGQGS------------------------------------ASQVSNQNIAKAQ 626
AI +L G+ A + SN N +
Sbjct: 555 KAIQEWLRTGNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGS 614
Query: 627 LYH--------------------------------------------SISGRDSKSEILG 686
S+ + + I+
Sbjct: 615 SCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVN 674
Query: 687 LIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKTGVQYTMLKDLIMDQLLTSISTSK 746
++ IS LC SE L EE ++++ + K + + + K I++ L+ +S S+
Sbjct: 675 SLKPYISSLCTSENLPEC-EEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 734
Query: 747 EEKVIRASVSLLTTIISENNSVIEDLKKKGLQLCDLATALRQNVHEAAILIYLISPSPRE 806
+V+R S+ +L+ +I ++SV E L LAT L+ + EAA+LIY + P +
Sbjct: 735 NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQ 794
Query: 807 IKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISS 866
+ + +L+P LV++I +K L++ P A++ I+E + D+ + ++ + S
Sbjct: 795 LSAHELIPSLVDVI-QNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVIS 854
Query: 867 PSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECRGYISKFIPVAQFLCLLQSDKKEA 926
+ + L V +EG S+ S+L+ CMQ + C+ I+ I ++ L L S
Sbjct: 855 ANGIPTL--VKYLERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSV 914
Query: 927 VHIALQVFNEILRVPRNILLNLNCLLKAVPLTQFSFILFRSSAISLLQRINNEGKNDIIH 986
++ +E++++ R R+S +L I +EG +H
Sbjct: 915 RGTCVEFLSELVQLNR-----------------------RTSCNQILHTIKDEGAFSTMH 974
Query: 987 ILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSAMQSLS 1046
++ + E+QL A++L+QL +L S+ +EEAV+ L+ ++ ++ S Q +
Sbjct: 975 TFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQMKA 1034
Query: 1047 ASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMIK------SFNWFDQSLQDA--GV 1106
L L G S +G+ YT AWLL+ G + ++K N ++++D +
Sbjct: 1035 LDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNAL 1094
Query: 1107 DSWCSLIARNIICIGE--PVFHALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSP-SSTRF 1166
+SW IA +++C E +F AL+ LKS K+++ CL WL + P + R
Sbjct: 1095 NSWQKRIA-SVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRD 1154
Query: 1167 SACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM-QKLANFSEGVRESLRRLSHI 1226
A + LL + L LEE++LA L + + S + L +++ + +LRRL
Sbjct: 1155 VARKSLLEEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKY 1214
Query: 1227 TWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1286
+ +A ++ +V L + +V+EL +S+G V ++++ G + G++DG+IKV
Sbjct: 1215 SVVAVDIMKVILNLKSVDVTELWSCKEVVELDLSSNGEVLSMVYLNGQVLSGHTDGTIKV 1274
Query: 1287 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIES 1346
W+ + + ++ + +H KAVT S SG+ L SGS DKTIRVW + ++C +V +
Sbjct: 1275 WDARKRIPRVIQETHEHTKAVT--SLCSSGDRLYSGSLDKTIRVWTIKSDGIKCIDVYDI 1334
BLAST of MS014628 vs. ExPASy Swiss-Prot
Match:
D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)
HSP 1 Score: 278.1 bits (710), Expect = 5.3e-73
Identity = 346/1515 (22.84%), Postives = 642/1515 (42.38%), Query Frame = 0
Query: 27 IRALVVSINQYIHEFLSNAEARIALTLRCTSKL-----RNQRHGFFEFLEQSIISNLYWG 86
+R L +I+ +I + L N E R +C +L + E+ +Q++++NL WG
Sbjct: 15 VRFLTTTIDSFIQDRLINKEQRTQHKDQCAERLAAEDGNTDKETEVEYSDQAVLANLDWG 74
Query: 87 IENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSGTENRYLVCCSYFYLSVVKK 146
IE +E+A+ T E + RL AE+MLQV A+L+ +T+G N YL ++ LS + K
Sbjct: 75 IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKTAGVPNSYLSAWAHLNLSYLWK 134
Query: 147 LQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFATS----SRQENGETMRS--D 206
L+ + H L+ +V P +FA +L L +S +E + M
Sbjct: 135 LRNNIKSCIYHSLEMFIVDPFFSRIDFAPELWKNLFLPHMSSIVGWYSEERHKLMMEVLP 194
Query: 207 SSVEFG-----EGDYGESTIRQVARKYKDWLMYYQVMLYGE-----TQQWKQDGSNCMLP 266
S +F + + ES + + + L + LYGE T+ + + ++CM P
Sbjct: 195 ESTDFSYTADFDKVFNESLVFSMRPNQLEKLQKLE-QLYGESLDENTRLYAKYYNDCMNP 254
Query: 267 PENGSHS--------------MSEASKAT------------DCGFPLPTLFHYDNIHPLD 326
S + E S++ GF + T D ++
Sbjct: 255 DSTSSKKVVPMLPIAEPPMTPLHELSRSVPDFVKFGPILPKSSGFSMTTRRSNDGLNETT 314
Query: 327 RTDVIQDK--TKASQDFPWCEDKGNSQKKLGLIPELQFNDRGSWRDSST----------- 386
R ++ + +K Q W K +I E++ + D+S
Sbjct: 315 RENIASNSNHSKGEQSSLWA-------AKESIIEEIEDDLDSEHYDASVDSDKINIFSPE 374
Query: 387 --KYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMNYTNHSKRS-TRADLPEKFSQKL 446
K I+D +D +P S + S + S +E +N+S + R KF + L
Sbjct: 375 PKKNIKD--EDVEPKVYRSNQKNQMNSPNISPMESPRRASNYSSTNPLRRKKESKFLRLL 434
Query: 447 RYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSKKSSDFKLRSIEQKNLEPQILQ 506
T S +S S + S + M ++ K+ +D + S+ Q N +L
Sbjct: 435 SNRFTGSIVSDHSLSSSPDTSSDHIFTGDEEVMVRNNIKRKNDSQTPSMNQDNENSLVLN 494
Query: 507 NC--LEESEPMELSLKLSKFQ--TFGS------------------------------AIP 566
+ E + + S K + T GS AI
Sbjct: 495 DSSHCESEDGYQSSSSFPKLEKLTIGSKPPKDFVCPITGQIFSDPVTLETGQTYERKAIQ 554
Query: 567 SFLGQGS------------------------------------ASQVSNQNIAK------ 626
+LG G+ A + SN N +
Sbjct: 555 EWLGTGNTTCPITRQALSANILPKTNYVLKRLIVSWKEQNPELAQEFSNSNTPRGSSCSP 614
Query: 627 ------------------AQLYH---------------------SISGRDSKSEILGLIE 686
+Q Y S+ + + I+ +
Sbjct: 615 SAKDITMVSSIQRTTDSPSQKYKDDYIRQRNNRFTRVSVGASPTSVLSQAAVETIINSLT 674
Query: 687 KAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEK 746
I+ LC SE L + E+ +E++ ++K + + + K ++ L+ +S S +
Sbjct: 675 PYITSLCTSENLQDC-EQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASLNRE 734
Query: 747 VIRASVSLLTTIISENNSVIEDLKKKGLQLCDLATALRQNVHEAAILIYLISPSPREIKS 806
V+R S+ +L+ +I + V E L LA L+ + EAA+LIY + P ++
Sbjct: 735 VLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNGLAEAALLIYQLRPVFAQLSE 794
Query: 807 LQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSV 866
+L+P L+++I +K + L + P AA++ I+E ++ D+ ++ + S +
Sbjct: 795 HELIPSLIQVI-QNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVISANG 854
Query: 867 LCGLLEVARTNNVEGLVSLGSILVKCMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHI 926
+ + V + EG + SIL+ CMQ + C+ I+ I ++ L L + I
Sbjct: 855 IPAI--VKYLDKTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLELFHAGNDSVRGI 914
Query: 927 ALQVFNEILRVPRNILLNLNCLLKAVPLTQFSFILFRSSAISLLQRINNEGKNDIIHILM 986
++ +E++R+ R R+S+ LQ I +EG +H +
Sbjct: 915 CVEFLSELVRLNR-----------------------RTSSNQTLQIIKDEGAFSTMHTFL 974
Query: 987 LCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASI 1046
+ + E+Q+ A++L+QL +L S+ +EEAV+ L+ ++ ++ S Q +
Sbjct: 975 VYLQMAPMEHQIAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNNQMKALDA 1034
Query: 1047 LSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMIK------SFNWFDQSLQDA--GVDSW 1106
L L G + +G+ YT A LL+ G + ++K S N F ++++D + SW
Sbjct: 1035 LLFLIGHVTSSGKSYTEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDEKNAMKSW 1094
Query: 1107 CSLIARNIICIGE--PVFHALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSP-SSTRFSAC 1166
+A +++C E +F AL+ LKS K+++ CL WL + P + R A
Sbjct: 1095 QKRVA-SVLCNHENGSIFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTGVRDVAR 1154
Query: 1167 EILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQK-LANFSEGVRESLRRLSHITWM 1226
+ LL + L LEE++LA L + ++ S + + L +++ + LR+L + +
Sbjct: 1155 KSLLEALMNVLQSSKNLEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTV 1214
Query: 1227 AEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKVWNI 1286
A ++ + L + +V+EL +S+G V +L + G + G++DG+IKVW+
Sbjct: 1215 AADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGHADGTIKVWDA 1274
Query: 1287 KGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIESKEQ 1346
+ + ++ + ++H+KAVT S S + L S S DKTIRVW + ++C +V + KE
Sbjct: 1275 RKRIPRVIQETREHKKAVT--SLCSSVDKLYSSSLDKTIRVWTIKPDGIKCIDVYDVKEA 1334
BLAST of MS014628 vs. ExPASy Swiss-Prot
Match:
D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)
HSP 1 Score: 271.6 bits (693), Expect = 4.9e-71
Identity = 350/1518 (23.06%), Postives = 642/1518 (42.29%), Query Frame = 0
Query: 27 IRALVVSINQYIHEFLSNAEARIALTLRCTSKL-----RNQRHGFFEFLEQSIISNLYWG 86
+R L +++ +I + L N E R +C +L + E+ +Q++++NL WG
Sbjct: 15 VRFLTTTVDSFIQDRLINKEQRTQHKEQCAERLAAEDGSGDKDTEVEYSDQAVLANLDWG 74
Query: 87 IENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSGTENRYLVCCSYFYLSVVKK 146
IE +E+A+ T E + RL AE+MLQV A+L+ + +G N YL ++ LS + K
Sbjct: 75 IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKIAGVPNSYLSAWAHLNLSYLWK 134
Query: 147 LQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFATS-----SRQENG---ETMR 206
L+ + H L+ +V P +FA SL L +S S + + E +
Sbjct: 135 LRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVGWYSEERHRLMMEVIP 194
Query: 207 SDSSVEFG---EGDYGESTIRQVARKYKDWLMYYQVMLYGE-----TQQWKQDGSNCMLP 266
+ + F E + ES + + + L + LYGE T+ + + ++CM
Sbjct: 195 DSADLSFTADFEQFFNESLVLTMRPHQLEKLQKLE-QLYGESLDENTKLYAKYYNDCM-- 254
Query: 267 PENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGNSQK 326
+ S S +A P T +H L RT I D K P + +
Sbjct: 255 -NSDSSSSKKAVPMLPIAEPPMT-----PLHELSRT--IPDFVKFGPILPKSAGFSLAPR 314
Query: 327 KLGLIPE----------LQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFS-SMNGSESD 386
++ E L+ W T ++++++ S + L + S++ + +
Sbjct: 315 SKDVLNETIRENVTSSNLKEEKLSIWGAKDT-----IIEENEDDSDSELDNESVDSDDKN 374
Query: 387 SDIEEGMNYTNHSKRSTRADL---------PEKFS--QKLRYACTKSDPEQSLISLSSA- 446
+ GM + T+ DL P+ FS R A S P + S +
Sbjct: 375 NIFSPGMKMMKYEGVETKVDLSCQRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSK 434
Query: 447 ----SLSTVQEQYIKVSMTKSFS-------------------KKSSDFKLRSIEQKNLEP 506
S S ++E I S+T S K+ +D + S+ Q N
Sbjct: 435 FLRLSSSRIREPTISDSLTSSPDISIDNISNADNEVMVRNNIKRKNDSQTPSMNQDNENS 494
Query: 507 QILQNC--LEESEPMELSLKLSKFQ--TFGS----------------------------- 566
+L + E + + S L K + + GS
Sbjct: 495 LVLNDSSHCESEDGYQSSSSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYER 554
Query: 567 -AIPSFLGQGS------------------------------------ASQVSNQNIAKAQ 626
AI +L G+ A + SN N +
Sbjct: 555 KAIQEWLRTGNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGS 614
Query: 627 LYH--------------------------------------------SISGRDSKSEILG 686
S+ + + I+
Sbjct: 615 SCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVN 674
Query: 687 LIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKTGVQYTMLKDLIMDQLLTSISTSK 746
++ IS LC SE L EE ++++ + K + + + K I++ L+ +S S+
Sbjct: 675 SLKPYISSLCTSENLPEC-EEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 734
Query: 747 EEKVIRASVSLLTTIISENNSVIEDLKKKGLQLCDLATALRQNVHEAAILIYLISPSPRE 806
+V+R S+ +L+ +I ++SV E L LAT L+ + EAA+LIY + P +
Sbjct: 735 NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQ 794
Query: 807 IKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISS 866
+ + +L+P LV++I +K L++ P A++ I+E + D+ + ++ + S
Sbjct: 795 LSAHELIPSLVDVI-QNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVIS 854
Query: 867 PSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECRGYISKFIPVAQFLCLLQSDKKEA 926
+ + L V +EG S+ S+L+ CMQ + C+ I+ I ++ L L S
Sbjct: 855 ANGIPTL--VKYLERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSV 914
Query: 927 VHIALQVFNEILRVPRNILLNLNCLLKAVPLTQFSFILFRSSAISLLQRINNEGKNDIIH 986
++ +E++++ R R+S LL I +EG +H
Sbjct: 915 RGTCVEFLSELVQLNR-----------------------RTSCNQLLHTIKDEGAFSTMH 974
Query: 987 ILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSAMQSLS 1046
++ + E+QL A++L+QL +L S+ +EEAV+ L+ ++ ++ S Q +
Sbjct: 975 TFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQMKA 1034
Query: 1047 ASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMIK------SFNWFDQSLQDA--GV 1106
L L G S +G+ YT AWLL+ G + ++K N ++++D +
Sbjct: 1035 LDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNAL 1094
Query: 1107 DSWCSLIARNIICIGE--PVFHALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSP-SSTRF 1166
+SW IA +++C E +F AL+ LKS K+++ CL WL + P + R
Sbjct: 1095 NSWQKRIA-SVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRD 1154
Query: 1167 SACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQK-LANFSEGVRESLRRLSHI 1226
A + LL + LH LE+ +L L ++ + S + + L +++ + LR+L
Sbjct: 1155 VARKSLLEEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKY 1214
Query: 1227 TWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1286
+ +A ++ + L + +V+EL +S+G V +L + G + G DG+ KV
Sbjct: 1215 STVAADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGLMDGTSKV 1274
Query: 1287 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIES 1346
+ + + ++ + +H KAVT S SG+ L S S DKTIRVW + ++C +V +
Sbjct: 1275 CDARKRIPRVIQETHEHTKAVT--SLCSSGDRLYSASLDKTIRVWTIKSDGIKCIDVYDI 1334
BLAST of MS014628 vs. ExPASy Swiss-Prot
Match:
P90648 (Myosin heavy chain kinase B OS=Dictyostelium discoideum OX=44689 GN=mhkB PE=2 SV=1)
HSP 1 Score: 68.6 bits (166), Expect = 6.4e-10
Identity = 70/272 (25.74%), Postives = 124/272 (45.59%), Query Frame = 0
Query: 1042 RVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKVWNIKGQSA 1101
RV DY N + CV T G V ++ + LF G SD SIKVW++K
Sbjct: 483 RVYDYKSQN---MECVQTLK-----GHEGPVESICYNDQYLFSGSSDHSIKVWDLK--KL 542
Query: 1102 SLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIESKEQ-IQHL 1161
++ ++ H K V + + + L SGS+DKTI+VW + LEC +ES + ++ L
Sbjct: 543 RCIFTLEGHDKPV--HTVLLNDKYLFSGSSDKTIKVWDL--KTLECKYTLESHARAVKTL 602
Query: 1162 GAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN----LKCIKVVQARVYAGCTDSSIQEF 1221
GQ +F+ ++ +KV D +T + + K + I ++ +Y+G D +I+ +
Sbjct: 603 CISGQYLFSGSNDKTIKVWD-LKTFRCNYTLKGHTKWVTTICILGTNLYSGSYDKTIRVW 662
Query: 1222 SVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTI 1281
++ + + + H + + + + LF+AS + + W T
Sbjct: 663 NLKSL-------ECSATLRGHDRWVEHMVICDKLLFTAS---DDNTIKIWDLETLRCNTT 722
Query: 1282 VTGKGDIVHAMSAVEDFVYLISKSSANSIQIW 1309
+ G V ++ ED +IS S SI++W
Sbjct: 723 LEGHNATVQCLAVWEDKKCVISCSHDQSIRVW 729
BLAST of MS014628 vs. ExPASy Swiss-Prot
Match:
Q9NWT1 (p21-activated protein kinase-interacting protein 1 OS=Homo sapiens OX=9606 GN=PAK1IP1 PE=1 SV=2)
HSP 1 Score: 58.9 bits (141), Expect = 5.0e-07
Identity = 42/150 (28.00%), Postives = 64/150 (42.67%), Query Frame = 0
Query: 1069 SGAVCALIFYKGL-LFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLL 1128
SG + L FY L G DG I +W+ K L IK H+ VT S SG+ L
Sbjct: 82 SGTITCLKFYGNRHLISGAEDGLICIWDAK--KWECLKSIKAHKGQVTFLSIHPSGKLAL 141
Query: 1129 SGSTDKTIRVWKMIQGKLECTEVIESKEQIQHLGAYG-QIIFAITHGHGLKVIDATRTTK 1188
S TDKT+R W +++G+ + I+ I G Q + I + + +D +
Sbjct: 142 SVGTDKTLRTWNLVEGRSAFIKNIKQNAHIVEWSPRGEQYVVIIQNKIDIYQLDTASISG 201
Query: 1189 VLFKSKNLKCIKVVQARVYAGCTDSSIQEF 1217
+ K + +K + V A D + F
Sbjct: 202 TITNEKRISSVKFLSESVLAVAGDEEVIRF 229
BLAST of MS014628 vs. ExPASy TrEMBL
Match:
A0A6J1C7S7 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009156 PE=4 SV=1)
HSP 1 Score: 2572.7 bits (6667), Expect = 0.0e+00
Identity = 1316/1343 (97.99%), Postives = 1317/1343 (98.06%), Query Frame = 0
Query: 3 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ 62
MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ
Sbjct: 1 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ 60
Query: 63 RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG 122
RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG
Sbjct: 61 RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG 120
Query: 123 TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT 182
TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT
Sbjct: 121 TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT 180
Query: 183 SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNC 242
SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSN
Sbjct: 181 SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNS 240
Query: 243 MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN 302
MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN
Sbjct: 241 MLPPENGSHSMSEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCEDKGN 300
Query: 303 SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN 362
SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN
Sbjct: 301 SQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEEGMN 360
Query: 363 YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK 422
YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK
Sbjct: 361 YTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKSFSK 420
Query: 423 KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN 482
KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN
Sbjct: 421 KSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQVSN 480
Query: 483 QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT 542
QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT
Sbjct: 481 QNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLNSKT 540
Query: 543 GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGLQLCDLA 602
GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGL+LCDLA
Sbjct: 541 GVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGLKLCDLA 600
Query: 603 TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM 662
TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM
Sbjct: 601 TALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAASMM 660
Query: 663 IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR 722
IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR
Sbjct: 661 IIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDGECR 720
Query: 723 GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKAVPLTQFSFI 782
GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVP
Sbjct: 721 GYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVP---------------------- 780
Query: 783 LFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK 842
RSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK
Sbjct: 781 --RSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLK 840
Query: 843 EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI 902
EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI
Sbjct: 841 EEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMI 900
Query: 903 KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG 962
KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG
Sbjct: 901 KSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLG 960
Query: 963 CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG 1022
CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG
Sbjct: 961 CEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEG 1020
Query: 1023 VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL 1082
VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL
Sbjct: 1021 VRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLL 1080
Query: 1083 FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ 1142
FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ
Sbjct: 1081 FGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQ 1140
Query: 1143 GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA 1202
GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA
Sbjct: 1141 GKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQA 1200
Query: 1203 RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL 1262
RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL
Sbjct: 1201 RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSL 1260
Query: 1263 FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG 1322
FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG
Sbjct: 1261 FQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAG 1319
Query: 1323 SRITCLLTANDMVLCGTETGKIK 1346
SRITCLLTANDMVLCGTETGK K
Sbjct: 1321 SRITCLLTANDMVLCGTETGKTK 1319
BLAST of MS014628 vs. ExPASy TrEMBL
Match:
A0A6J1GTU3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita moschata OX=3662 GN=LOC111457509 PE=4 SV=1)
HSP 1 Score: 2195.2 bits (5687), Expect = 0.0e+00
Identity = 1127/1352 (83.36%), Postives = 1197/1352 (88.54%), Query Frame = 0
Query: 2 CMAP--ASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CMA +SS TQ PP VCEN RLDLNSIR LVVSINQYIHEFLSNAEAR A+ LRC SKL
Sbjct: 5 CMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRCISKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RN +H +FEFLEQSIISNLYWG+EN+EDAVQTS SE RAT+LQTAEQMLQVPALLDEHGE
Sbjct: 65 RNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATKLQTAEQMLQVPALLDEHGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG +NRYLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLS
Sbjct: 125 TSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
FAT SRQEN +MRS+S VEFGEGD+GEST+RQVARKYKDWLMYYQVM YGET QW+Q G
Sbjct: 185 FATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S+ M E+GSHS+ E SKA DCG PT+ HYD I PLD DV QDKT SQD
Sbjct: 245 SSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTNTSQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
P CE+ GNS+K LGL+PE Q ND G WRDSSTK+I DLLKDS GSPTSLFSSMN SES
Sbjct: 305 VPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMNDSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD E GMNYTNHSKRS + D+PE F QKLRYA +KSD EQSLISL+SASLS VQE YI+
Sbjct: 365 DSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQEHYIE 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K +D+KL S EQK+LEPQILQNCLE+SEP EL + K QTF SA+P LG
Sbjct: 425 ANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALPLSLG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGS Q+S +N AK QLYH+ S +DSKSEILGL+EKAISRLCFSEGLGNYD+EYAVEVST
Sbjct: 485 QGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEII TS+CYN WSP M
Sbjct: 605 KGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWSPPFM 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECR YISKFI VA FLCLLQSDKKEAVHI LQVFNEILRVP
Sbjct: 725 CMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEG NDIIHILMLCVNHLQTEYQLLAAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
N STTS LKEEAV VLLRSVACEE SAMQ LSASILS LGGTF+WTGEPYTVAWLL+KVG
Sbjct: 845 NGSTTSFLKEEAVHVLLRSVACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCS++ARNIICIGEPVFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSVMARNIICIGEPVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
LTTIAWLGCEIAKSPSS R SACEILL IELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 SLTTIAWLGCEIAKSPSSIRCSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
Q L NFSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QNLTNFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQHLGAYGQIIFAITHGHGLKVIDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKC+KVVQ RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLF
Sbjct: 1205 LKCMKVVQGRVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFC 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASS+VQGSLFQNWRRH+KP+M IVTGKGD+V AMS VEDFVY+I KSS +SIQIWLRK Q
Sbjct: 1265 ASSIVQGSLFQNWRRHEKPKMNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
HKVGR+SA SRITCLLTANDMVLCGTETGKIK
Sbjct: 1325 HKVGRVSASSRITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. ExPASy TrEMBL
Match:
A0A0A0L5U3 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G159430 PE=4 SV=1)
HSP 1 Score: 2185.2 bits (5661), Expect = 0.0e+00
Identity = 1123/1351 (83.12%), Postives = 1195/1351 (88.45%), Query Frame = 0
Query: 1 ICMAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLR 60
I + +SS TQ P VCENER+DLNSIR LVVSINQYIHEFLSNAEAR A+ LRCTSKLR
Sbjct: 8 IASSSSSSTTQCPLPVCENERVDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRCTSKLR 67
Query: 61 NQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGET 120
NQR GF EFLEQSIISNLYWGIEN+EDAVQTS SE RATRLQTAEQMLQVPAL+DEHGET
Sbjct: 68 NQRPGFLEFLEQSIISNLYWGIENIEDAVQTSSSEARATRLQTAEQMLQVPALVDEHGET 127
Query: 121 SGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSF 180
SG EN YLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLSF
Sbjct: 128 SGMENCYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLSF 187
Query: 181 ATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGS 240
AT SRQ+N +M +SSVEFGEGDYGES+IRQVARKYKDWLMYYQVM YGET QW+Q GS
Sbjct: 188 ATCSRQDNFRSMGFNSSVEFGEGDYGESSIRQVARKYKDWLMYYQVMSYGETHQWQQLGS 247
Query: 241 NCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDF 300
+ M E+G HS+ EAS+ATDCGFP PTL HYD I PLD DV QDK KASQDF
Sbjct: 248 SNMTSSEDGPHSLHGSFSRIEASEATDCGFPRPTLSHYDIIPPLDHIDVFQDKRKASQDF 307
Query: 301 PWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESD 360
P CED GNS K+LG IPE QFN+ G RDSSTK I D+LKDS PGSPTSLFSSMN SESD
Sbjct: 308 PRCEDTGNSPKELGFIPEPQFNNWGFCRDSSTKCIGDVLKDSHPGSPTSLFSSMNNSESD 367
Query: 361 SDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKV 420
SD E GMN NH K+S + D+PE F QKL+Y C+K D E SLISLSSASLS V+E+Y K
Sbjct: 368 SDFEAGMNDINHPKKSGQEDMPENFYQKLQYGCSKFDGEPSLISLSSASLSRVKERYSKA 427
Query: 421 SMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQ 480
+M KS S K + +K RSIEQKNL+PQ+ QN LEESEP + S+ K QTF S++PS GQ
Sbjct: 428 NMMKSISNKFNGYKSRSIEQKNLQPQVFQNFLEESEPKKKSVNPCKLQTFDSSLPSSFGQ 487
Query: 481 GSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTI 540
GSA + QN AK QLYH+ S RDSKSEILGL+EKAISRLCFSEGLGNYD+E AVEVST+
Sbjct: 488 GSACPILKQNSAKGQLYHANSRRDSKSEILGLVEKAISRLCFSEGLGNYDDECAVEVSTV 547
Query: 541 YKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKK 600
YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KKK
Sbjct: 548 YKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKKK 607
Query: 601 GLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLML 660
GLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEIICTSKCYNAWSPSLML
Sbjct: 608 GLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNAWSPSLML 667
Query: 661 TPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKC 720
TPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGL+SLGSILVKC
Sbjct: 668 TPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLMSLGSILVKC 727
Query: 721 MQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKAV 780
MQLDGECR Y SKFI VA FL LL+SDKKEAVHIALQVFNEIL VP
Sbjct: 728 MQLDGECRAYTSKFISVAPFLSLLESDKKEAVHIALQVFNEILHVP-------------- 787
Query: 781 PLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLEN 840
RSSAISLLQR+ NEGKND+IHILMLCVNHLQTEYQLLAAN+LIQLLVL+N
Sbjct: 788 ----------RSSAISLLQRVKNEGKNDVIHILMLCVNHLQTEYQLLAANLLIQLLVLDN 847
Query: 841 CSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGL 900
CSTTSLLKEEAVQVLLRSV CEE SAMQ LSASILS +GGTF+WTGEPYTVAWLL+KVGL
Sbjct: 848 CSTTSLLKEEAVQVLLRSVTCEESSAMQLLSASILSTIGGTFAWTGEPYTVAWLLKKVGL 907
Query: 901 SSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDC 960
SSDHQNMIKS NW DQSLQDAG+DSWCSL+ARNIICIGEPVFHAL++GLKS IKKVSRDC
Sbjct: 908 SSDHQNMIKSINWLDQSLQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSDIKKVSRDC 967
Query: 961 LTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQ 1020
LTTIAWLGCEIAKSP S R SACEILL GIELFLHPG+ELEERLLACLCIFNY SGKGMQ
Sbjct: 968 LTTIAWLGCEIAKSPDSIRCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQ 1027
Query: 1021 KLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCA 1080
KL FSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVCA
Sbjct: 1028 KLTRFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCA 1087
Query: 1081 LIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKT 1140
LIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCF+HFESGESLLSGS DKT
Sbjct: 1088 LIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFAHFESGESLLSGSADKT 1147
Query: 1141 IRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNL 1200
IRVWKMI G+LEC EVIESKEQIQHLGAYGQIIFA+THG+GLKVIDA+RTTKVLFKSKNL
Sbjct: 1148 IRVWKMIHGRLECIEVIESKEQIQHLGAYGQIIFAVTHGYGLKVIDASRTTKVLFKSKNL 1207
Query: 1201 KCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSA 1260
KCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSW++MH KAINSLAVYKDWLFSA
Sbjct: 1208 KCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWILMHQKAINSLAVYKDWLFSA 1267
Query: 1261 SSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQH 1320
SSMVQGSL QNWRRH+KPEM I+TGKGD+V AMS VEDFVY+I KSSANSIQIWLRK QH
Sbjct: 1268 SSMVQGSLLQNWRRHEKPEMNIITGKGDVVQAMSVVEDFVYIICKSSANSIQIWLRKAQH 1327
Query: 1321 KVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
KVGR SAGS+ITCLLTANDMVLCGTETGKIK
Sbjct: 1328 KVGRASAGSKITCLLTANDMVLCGTETGKIK 1334
BLAST of MS014628 vs. ExPASy TrEMBL
Match:
A0A6J1ITY3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita maxima OX=3661 GN=LOC111479321 PE=4 SV=1)
HSP 1 Score: 2182.9 bits (5655), Expect = 0.0e+00
Identity = 1122/1352 (82.99%), Postives = 1194/1352 (88.31%), Query Frame = 0
Query: 2 CMAPA--SSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKL 61
CMA A SS TQ PP VCEN RLDLNSIR LVVSINQYIHEFLSNAEAR ++ LRC SKL
Sbjct: 5 CMATAFSSSPTQCPPPVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTSVKLRCISKL 64
Query: 62 RNQRHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGE 121
RN +H +FEFLEQSIISNLYWG+EN+ED VQTS SE RAT+LQTAEQMLQVPALLDEHGE
Sbjct: 65 RNHKHEYFEFLEQSIISNLYWGVENIEDTVQTSSSEVRATKLQTAEQMLQVPALLDEHGE 124
Query: 122 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 181
TSG +NRYLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLS
Sbjct: 125 TSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLS 184
Query: 182 FATSSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDG 241
FAT SRQEN +MRS+S VEFGEGD+GEST+RQVARKYKDWLMYYQVM YGET QW+Q G
Sbjct: 185 FATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQWQQQG 244
Query: 242 SNCMLPPENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQD 301
S+ M E+GSHS+ E SKA DCG PT+ HYD I PLD D QDK SQD
Sbjct: 245 SSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAKPTVSHYDIISPLDHIDAFQDKINTSQD 304
Query: 302 FPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSES 361
P CE+ GNS+K LGLIPE Q ND G WRDSSTK+I D+LKDS GSPTSLFSSMN SES
Sbjct: 305 VPRCEELGNSEKNLGLIPEPQLNDGGFWRDSSTKFIGDMLKDSHLGSPTSLFSSMNDSES 364
Query: 362 DSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIK 421
DSD E GMNYTNHSKRS + D+PE F QKLRYA +KSD EQSLISL+SASLS VQE YI+
Sbjct: 365 DSDFEAGMNYTNHSKRSAQEDVPENFYQKLRYARSKSDTEQSLISLTSASLSRVQEHYIE 424
Query: 422 VSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLG 481
+M KS S K +D+KL S EQK+LEPQILQNCLE+SEP EL + K QTF SA+P LG
Sbjct: 425 ANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELFVNPCKLQTFDSALPLALG 484
Query: 482 QGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVST 541
QGS Q+S QN AK QLYH+ S +DSKSEILGL+EKAISRLCFSEGLGNYD+EYAVEVST
Sbjct: 485 QGSTCQISKQNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAVEVST 544
Query: 542 IYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKK 601
+YKMLN+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KK
Sbjct: 545 VYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKK 604
Query: 602 KGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLM 661
KGLQLCDLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEII TS+CYN WSP LM
Sbjct: 605 KGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWSPPLM 664
Query: 662 LTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 721
LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK
Sbjct: 665 LTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVK 724
Query: 722 CMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKA 781
CMQLDGECR YISKFI VA FLCLLQSDKKEAVHI LQVFNEILRVP
Sbjct: 725 CMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVP------------- 784
Query: 782 VPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLE 841
RSSAISLLQRI NEG NDIIHILMLCV+HLQTEYQLLAAN+LIQLLVL+
Sbjct: 785 -----------RSSAISLLQRIKNEGGNDIIHILMLCVDHLQTEYQLLAANLLIQLLVLD 844
Query: 842 NCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVG 901
N STTS LKEEAV VLLRSVACEE SAMQ LSASILS LGGTF+WTGEPYTVAWLL+KVG
Sbjct: 845 NGSTTSFLKEEAVHVLLRSVACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVG 904
Query: 902 LSSDHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRD 961
LSSDHQNMIKSFNW DQSLQDAG+DSWCSL+ARNIICIGEPVFHAL++GLKS IKKVSRD
Sbjct: 905 LSSDHQNMIKSFNWLDQSLQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRD 964
Query: 962 CLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGM 1021
CLTTIAWLGCEIAKSPSS R SACEILL IELFLHPG+ELEERLLACLCIFNY SGKGM
Sbjct: 965 CLTTIAWLGCEIAKSPSSIRCSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGM 1024
Query: 1022 QKLANFSEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1081
Q L NFSEGVRESLRRLSHITWMAEELH+VADYLMPNNSRISCVHTQVLELGFNSSGAVC
Sbjct: 1025 QNLTNFSEGVRESLRRLSHITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVC 1084
Query: 1082 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDK 1141
ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DK
Sbjct: 1085 ALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDK 1144
Query: 1142 TIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKN 1201
TIRVWKMIQG+LEC EVIESKEQIQH+GAYGQIIFAITHGHGLK+IDA+RTTKVLFKSKN
Sbjct: 1145 TIRVWKMIQGRLECIEVIESKEQIQHVGAYGQIIFAITHGHGLKMIDASRTTKVLFKSKN 1204
Query: 1202 LKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFS 1261
LKC+KVVQARVYAGCTDSSIQEFSVTNKWEQEIK PSKSWMMMHHKAINSLAVYKDWLF
Sbjct: 1205 LKCMKVVQARVYAGCTDSSIQEFSVTNKWEQEIKSPSKSWMMMHHKAINSLAVYKDWLFC 1264
Query: 1262 ASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQ 1321
ASS+VQGSLFQNWRRH+KP+M IVT KGD+V A+S VEDFVY+I KSS +SIQIWLRK Q
Sbjct: 1265 ASSIVQGSLFQNWRRHEKPKMNIVTSKGDVVQAISVVEDFVYIICKSSLSSIQIWLRKAQ 1324
Query: 1322 HKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
KVGR+SA SRITCLLTANDMVLCGTETGKIK
Sbjct: 1325 LKVGRVSASSRITCLLTANDMVLCGTETGKIK 1332
BLAST of MS014628 vs. ExPASy TrEMBL
Match:
A0A1S3AXG3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucumis melo OX=3656 GN=LOC103483854 PE=4 SV=1)
HSP 1 Score: 2149.0 bits (5567), Expect = 0.0e+00
Identity = 1110/1346 (82.47%), Postives = 1188/1346 (88.26%), Query Frame = 0
Query: 6 ASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQRHG 65
+SS TQ P VCENERLD NSIR LVVSINQYIHEFLSNAE R A+ LRCTSKLRNQR G
Sbjct: 12 SSSPTQCPLPVCENERLDPNSIRGLVVSINQYIHEFLSNAEVRTAVKLRCTSKLRNQRPG 71
Query: 66 FFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSGTEN 125
F EFLEQSIISNLYWGIEN+EDAVQTS SE +ATRLQTAEQMLQVPAL+DEHGETSG EN
Sbjct: 72 FLEFLEQSIISNLYWGIENIEDAVQTSSSEAKATRLQTAEQMLQVPALVDEHGETSGMEN 131
Query: 126 RYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFATSSR 185
RYLVCCSYFYLS+VKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQ FCYSLLLSFAT SR
Sbjct: 132 RYLVCCSYFYLSIVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYSLLLSFATCSR 191
Query: 186 QENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNCMLP 245
QEN +M + SVEFGEGDYGES+IRQVARKYKDWLMYYQVM YGET QW+Q GS+ M+
Sbjct: 192 QENFRSMGFNPSVEFGEGDYGESSIRQVARKYKDWLMYYQVMSYGETHQWQQLGSSNMMS 251
Query: 246 PENGSHSMS------EASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPWCED 305
E+GS S+ E S+ATDC F PTL HYD I PLD DV QDK KASQ+FP CED
Sbjct: 252 SEDGSLSLHGSFSRIETSEATDCRFSRPTLSHYDIIPPLDHIDVFQDKRKASQNFPRCED 311
Query: 306 KGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSDIEE 365
NS K LG PE QFND G RDSSTK + D+LKDS PGSPTSLFSSMN SESDSD E
Sbjct: 312 TVNSPKNLGFFPEPQFNDWGFCRDSSTKCMGDVLKDSHPGSPTSLFSSMNNSESDSDFEA 371
Query: 366 GMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSMTKS 425
GMN NH K+S +AD+P+ QKL+Y C+KSD EQSLISLSSASLS V+E+Y K +M KS
Sbjct: 372 GMNDINHPKKSGQADMPD--YQKLQYCCSKSDREQSLISLSSASLSRVKERYTKANMMKS 431
Query: 426 FSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGSASQ 485
S K + +K RS+E+ NLE Q+ QN LEESEP ++S+ L K QTF S +PS L QGSA Q
Sbjct: 432 ISNKFNGYKSRSLEKNNLETQVFQNFLEESEPKDMSVNLCKLQTFDSYLPSSLDQGSACQ 491
Query: 486 VSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYKMLN 545
+ QN + QL H+ S RD KSEILGL+EKAISRLCFSEGLGNYD+E AVEVST+YKMLN
Sbjct: 492 IRKQN-SGGQLCHANSRRDPKSEILGLVEKAISRLCFSEGLGNYDDECAVEVSTVYKMLN 551
Query: 546 SKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGLQLC 605
+KTGVQYTMLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENNSVIED+KKKGLQLC
Sbjct: 552 NKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIEDIKKKGLQLC 611
Query: 606 DLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTPPAA 665
DLATAL+QNVHEAAILIYLISPSPREIKSL+LLPVLVEIICTSKCYNAWSPSL LTPPAA
Sbjct: 612 DLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNAWSPSLTLTPPAA 671
Query: 666 SMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQLDG 725
SMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTNNVEGL+SLGSILVKCMQLDG
Sbjct: 672 SMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLMSLGSILVKCMQLDG 731
Query: 726 ECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRNILLNLNCLLKAVPLTQF 785
ECR Y SKFI VA FLCLL+SDKKEAVHIALQVFNEIL VP
Sbjct: 732 ECRSYTSKFISVAPFLCLLESDKKEAVHIALQVFNEILHVP------------------- 791
Query: 786 SFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTS 845
RSSAISLLQRI NEGKND+IHILMLCVNHLQTEYQLLAAN+LIQLLVL+NCSTTS
Sbjct: 792 -----RSSAISLLQRIKNEGKNDVIHILMLCVNHLQTEYQLLAANLLIQLLVLDNCSTTS 851
Query: 846 LLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQ 905
LLKEEAVQVLLRSVACEE S+MQ LSASILS +GGTFSWTGEPYTVAWLL+KVGLSSDHQ
Sbjct: 852 LLKEEAVQVLLRSVACEETSSMQLLSASILSTIGGTFSWTGEPYTVAWLLKKVGLSSDHQ 911
Query: 906 NMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIA 965
NMIKSF+W DQSLQDAG+DSWCSL+ARNIICIGEPVF AL++GLKS IKKVSRDCLTTIA
Sbjct: 912 NMIKSFDWLDQSLQDAGMDSWCSLMARNIICIGEPVFRALEKGLKSDIKKVSRDCLTTIA 971
Query: 966 WLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANF 1025
WLGCEIAKSP+S R SACEILL GIELFLHPG+ELEERLLACLCIFNY SGKGMQKL F
Sbjct: 972 WLGCEIAKSPNSIRCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTRF 1031
Query: 1026 SEGVRESLRRLSHITWMAEELHRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYK 1085
SEGVRESLRRLSHITWMAEELH+VADYLMP+NSRISCVHTQVLELGFNSSGAVCALIFYK
Sbjct: 1032 SEGVRESLRRLSHITWMAEELHQVADYLMPHNSRISCVHTQVLELGFNSSGAVCALIFYK 1091
Query: 1086 GLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWK 1145
GLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS DKTIRVWK
Sbjct: 1092 GLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWK 1151
Query: 1146 MIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKV 1205
MIQG+LEC EVIESKEQIQHLGAYGQIIFA+T+GHGLKVIDA+RTTKVLFKSKNLKCIKV
Sbjct: 1152 MIQGRLECIEVIESKEQIQHLGAYGQIIFAVTNGHGLKVIDASRTTKVLFKSKNLKCIKV 1211
Query: 1206 VQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQ 1265
VQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSW++MH KAINSLAVYKDWLFSASSMVQ
Sbjct: 1212 VQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWILMHQKAINSLAVYKDWLFSASSMVQ 1271
Query: 1266 GSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRI 1325
GSL QNWRRH+KPEM I+TGKG++V AMS VEDFVY+I KS ANSIQIWLRK QHKVGR+
Sbjct: 1272 GSLLQNWRRHEKPEMKIITGKGEVVQAMSVVEDFVYIICKSLANSIQIWLRKAQHKVGRV 1330
Query: 1326 SAGSRITCLLTANDMVLCGTETGKIK 1346
SAGS+ITCLLTANDMVLCGTETGKIK
Sbjct: 1332 SAGSKITCLLTANDMVLCGTETGKIK 1330
BLAST of MS014628 vs. TAIR 10
Match:
AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 1015.4 bits (2624), Expect = 4.3e-296
Identity = 609/1369 (44.49%), Postives = 831/1369 (60.70%), Query Frame = 0
Query: 3 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLR-N 62
MA SS S EN LD +++VSIN YI +S+ EA I+L +C + L
Sbjct: 1 MAYLSSSQSTFISCSENSSLD-----SILVSINVYILGVISDPEAWISLKQKCITLLSIE 60
Query: 63 QRHGFFEF-LEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHG-E 122
+ + FEF E S +SNLYWGI+++E ++ SEE+ +RL+ +E+MLQ+PALLDE G
Sbjct: 61 EDNTLFEFSSEHSALSNLYWGIDSIEASIHPECSEEKTSRLRNSERMLQMPALLDEQGTT 120
Query: 123 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 182
TSG N L+ SYFYLS+V LQGD Q LHFLQS+LVSP +V T+ A C
Sbjct: 121 TSGVPNTVLISFSYFYLSIVSYLQGDSLQSTLHFLQSVLVSPEIVRTDIAPELC------ 180
Query: 183 FATSSRQENGETMRSDSSVEFGEGDY-GESTIRQVARKYKDWLMYYQVMLYGETQQWKQD 242
S+ F G Y + IR++ARKYK YYQVM YGET Q ++
Sbjct: 181 ----------------ESIFFTPGVYKSDEEIREIARKYKYRATYYQVMSYGETHQPPRE 240
Query: 243 GSNCMLPPEN------------GSHSMSEASKATDCGFPLPTLFHYDNIHPLD----RTD 302
C+ P +HS++E + ++ L Y N+H +D +
Sbjct: 241 ---CIETPVRRQKEYGQEIFAANAHSVAEKLELSE---TCEKLLQYQNLHSVDLQEEELN 300
Query: 303 VIQDKTKASQDFPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPT 362
I +K KAS+ E+ + L + +N + + + + + L +SQP
Sbjct: 301 DIFNKIKASRKIEKSENNFEGSQCLDCNLQEDYNAEPG-KSTRVRCLNEFLNESQP---- 360
Query: 363 SLFSSMNGSESDSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSA 422
+ D + N S++ + + +++ T ++ S I +
Sbjct: 361 -------DTREDIGTDTLANIFCVSQQQAHKEANKAYNED-----TLANRSSSFIGNFNR 420
Query: 423 SLSTVQEQYIKVSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQ 482
S+ +Q Q K + S S+ Q +LE ++S F
Sbjct: 421 SIFEIQAQQSKTTWNTHLEDAS------SLRQLDLE------------------EISVFG 480
Query: 483 TFGSAIPSFLGQGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGN 542
GS SF G Q + L D + LI+ E LGN
Sbjct: 481 QKGSI--SFEGMRRNLQTKKRGNGLETLSRRAPTMDLWMNLQSLIK---------EVLGN 540
Query: 543 YDEEYAVEVSTIYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIIS 602
DE+Y EV+ IY+MLN K G +Y+MLKD+I+DQL T+IS+S+E+ VI+AS++ LT IIS
Sbjct: 541 ADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIIS 600
Query: 603 ENNSVIEDLKKKGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIIC-- 662
N + +E++K+KGL L LA AL+QNV EAAILIYLI PSP EIKSL+LLP LV+++
Sbjct: 601 VNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVAST 660
Query: 663 --TSKCYNAWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVART 722
+S CY LTPPAAS+MIIEV++TAFD TN MHL ISSPSVLCGLL+VA++
Sbjct: 661 SSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKS 720
Query: 723 NNVEGLVSLGSILVKCMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILR 782
N +SL SILVKCMQ DG R YI + VA F LLQS +E + IALQ +E+L+
Sbjct: 721 GNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLK 780
Query: 783 VPRNILLNLNCLLKAVPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEY 842
+P RSSAI +LQ+I EG DI L+ C+ HLQ ++
Sbjct: 781 IP------------------------RSSAIKILQQIKKEGSFDIKVTLLHCIKHLQGDH 840
Query: 843 QLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSW 902
+L AA++L+QL L++ + EA + LL +V E S MQ LS IL+N+GGT+SW
Sbjct: 841 KLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSW 900
Query: 903 TGEPYTVAWLLRKVGLSS-DHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFH 962
TGEPYT AWL+++ GL+S H NMI++ NW D+ LQD G+D WC IAR II G+ F
Sbjct: 901 TGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDECLQDTGIDGWCCKIARRIIDTGKATFC 960
Query: 963 ALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEER 1022
L GLKSK K VS+ CL IAWL EI+K P+S ++SACE+LL + FLHPG+ELEER
Sbjct: 961 GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKYSACEVLLDEVAQFLHPGLELEER 1020
Query: 1023 LLACLCIFNYASGKGMQKLANFSEGVRESLRRLSHITWMAEELHRVADYLM-PNNSRISC 1082
LLAC+CI+N++SGKG+ KL NFSEGVRESLRRLSH+TWMA+ELH+ YL ++ RISC
Sbjct: 1021 LLACICIYNFSSGKGIHKLVNFSEGVRESLRRLSHVTWMADELHKATYYLFSKSDQRISC 1080
Query: 1083 VHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTC 1142
VHTQ +E+ + SGAV ALI++KGLLF G+SDGSI+VWN+ + A+LLWDIK+H+ VTC
Sbjct: 1081 VHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSIRVWNVNKKIATLLWDIKEHKSTVTC 1140
Query: 1143 FSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGL 1202
FS E+GE +LSGS DKTIRVW++++GKLEC EVI++K+ I+ L A+G +IF IT GH +
Sbjct: 1141 FSLSETGECVLSGSADKTIRVWQIVKGKLECAEVIKTKDSIRKLEAFGNMIFVITKGHKM 1200
Query: 1203 KVIDATRTTKVLFKSKNLKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMM 1262
K++D++R ++ +FK K +K + Q ++Y GC D+SIQE V NK E+EIK P++SW +
Sbjct: 1201 KLLDSSRISQSIFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSW-RL 1259
Query: 1263 HHKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYL 1322
+K INS+ VYKD L+S+S+ V+ S ++ RR+ +P+M+I KG + AM VEDF+YL
Sbjct: 1261 QNKPINSVVVYKDMLYSSSTYVEMSNIKDLRRNYEPQMSITAEKGSNIVAMGVVEDFIYL 1259
Query: 1323 ISKSSANSIQIWLRKTQHKVGRISAGSRITCLLTANDMVLCGTETGKIK 1346
SSAN++QIWLR+TQ KVGR+SAGS+IT LLTAND+V CGTE G IK
Sbjct: 1321 NRSSSANTLQIWLRRTQQKVGRLSAGSKITSLLTANDIVFCGTEAGVIK 1259
BLAST of MS014628 vs. TAIR 10
Match:
AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 1012.7 bits (2617), Expect = 2.8e-295
Identity = 607/1366 (44.44%), Postives = 829/1366 (60.69%), Query Frame = 0
Query: 3 MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLR-N 62
MA SS S EN LD +++VSIN YI +S+ EA I+L +C + L
Sbjct: 1 MAYLSSSQSTFISCSENSSLD-----SILVSINVYILGVISDPEAWISLKQKCITLLSIE 60
Query: 63 QRHGFFEF-LEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHG-E 122
+ + FEF E S +SNLYWGI+++E ++ SEE+ +RL+ +E+MLQ+PALLDE G
Sbjct: 61 EDNTLFEFSSEHSALSNLYWGIDSIEASIHPECSEEKTSRLRNSERMLQMPALLDEQGTT 120
Query: 123 TSGTENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLS 182
TSG N L+ SYFYLS+V LQGD Q LHFLQS+LVSP +V T+ A C
Sbjct: 121 TSGVPNTVLISFSYFYLSIVSYLQGDSLQSTLHFLQSVLVSPEIVRTDIAPELC------ 180
Query: 183 FATSSRQENGETMRSDSSVEFGEGDY-GESTIRQVARKYKDWLMYYQVMLYGETQQWKQD 242
S+ F G Y + IR++ARKYK YYQVM YGET Q ++
Sbjct: 181 ----------------ESIFFTPGVYKSDEEIREIARKYKYRATYYQVMSYGETHQPPRE 240
Query: 243 GSNCMLPPEN------------GSHSMSEASKATDCGFPLPTLFHYDNIHPLD----RTD 302
C+ P +HS++E + ++ L Y N+H +D +
Sbjct: 241 ---CIETPVRRQKEYGQEIFAANAHSVAEKLELSE---TCEKLLQYQNLHSVDLQEEELN 300
Query: 303 VIQDKTKASQDFPWCEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPT 362
I +K KAS+ E+ + L + +N + + + + + L +SQP
Sbjct: 301 DIFNKIKASRKIEKSENNFEGSQCLDCNLQEDYNAEPG-KSTRVRCLNEFLNESQP---- 360
Query: 363 SLFSSMNGSESDSDIEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSA 422
+ D + N S++ + + +++ T ++ S I +
Sbjct: 361 -------DTREDIGTDTLANIFCVSQQQAHKEANKAYNED-----TLANRSSSFIGNFNR 420
Query: 423 SLSTVQEQYIKVSMTKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQ 482
S+ +Q Q K + S S+ Q +LE ++S F
Sbjct: 421 SIFEIQAQQSKTTWNTHLEDAS------SLRQLDLE------------------EISVFG 480
Query: 483 TFGSAIPSFLGQGSASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGN 542
GS SF G Q + L D + LI+ E LGN
Sbjct: 481 QKGSI--SFEGMRRNLQTKKRGNGLETLSRRAPTMDLWMNLQSLIK---------EVLGN 540
Query: 543 YDEEYAVEVSTIYKMLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIIS 602
DE+Y EV+ IY+MLN K G +Y+MLKD+I+DQL T+IS+S+E+ VI+AS++ LT IIS
Sbjct: 541 ADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIIS 600
Query: 603 ENNSVIEDLKKKGLQLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIIC-- 662
N + +E++K+KGL L LA AL+QNV EAAILIYLI PSP EIKSL+LLP LV+++
Sbjct: 601 VNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVAST 660
Query: 663 --TSKCYNAWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVART 722
+S CY LTPPAAS+MIIEV++TAFD TN MHL ISSPSVLCGLL+VA++
Sbjct: 661 SSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKS 720
Query: 723 NNVEGLVSLGSILVKCMQLDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILR 782
N +SL SILVKCMQ DG R YI + VA F LLQS +E + IALQ +E+L+
Sbjct: 721 GNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLK 780
Query: 783 VPRNILLNLNCLLKAVPLTQFSFILFRSSAISLLQRINNEGKNDIIHILMLCVNHLQTEY 842
+P RSSAI +LQ+I EG DI L+ C+ HLQ ++
Sbjct: 781 IP------------------------RSSAIKILQQIKKEGSFDIKVTLLHCIKHLQGDH 840
Query: 843 QLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSAMQSLSASILSNLGGTFSW 902
+L AA++L+QL L++ + EA + LL +V E S MQ LS IL+N+GGT+SW
Sbjct: 841 KLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSW 900
Query: 903 TGEPYTVAWLLRKVGLSS-DHQNMIKSFNWFDQSLQDAGVDSWCSLIARNIICIGEPVFH 962
TGEPYT AWL+++ GL+S H NMI++ NW D+ LQD G+D WC IAR II G+ F
Sbjct: 901 TGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDECLQDTGIDGWCCKIARRIIDTGKATFC 960
Query: 963 ALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSPSSTRFSACEILLGGIELFLHPGIELEER 1022
L GLKSK K VS+ CL IAWL EI+K P+S ++SACE+LL + FLHPG+ELEER
Sbjct: 961 GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKYSACEVLLDEVAQFLHPGLELEER 1020
Query: 1023 LLACLCIFNYASGKGMQKLANFSEGVRESLRRLSHITWMAEELHRVADYLM-PNNSRISC 1082
LLAC+CI+N++SGKG+ KL NFSEGVRESLRRLSH+TWMA+ELH+ YL ++ RISC
Sbjct: 1021 LLACICIYNFSSGKGIHKLVNFSEGVRESLRRLSHVTWMADELHKATYYLFSKSDQRISC 1080
Query: 1083 VHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTC 1142
VHTQ +E+ + SGAV ALI++KGLLF G+SDGSI+VWN+ + A+LLWDIK+H+ VTC
Sbjct: 1081 VHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSIRVWNVNKKIATLLWDIKEHKSTVTC 1140
Query: 1143 FSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIESKEQIQHLGAYGQIIFAITHGHGL 1202
FS E+GE +LSGS DKTIRVW++++GKLEC EVI++K+ I+ L A+G +IF IT GH +
Sbjct: 1141 FSLSETGECVLSGSADKTIRVWQIVKGKLECAEVIKTKDSIRKLEAFGNMIFVITKGHKM 1200
Query: 1203 KVIDATRTTKVLFKSKNLKCIKVVQARVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMM 1262
K++D++R ++ +FK K +K + Q ++Y GC D+SIQE V NK E+EIK P++SW +
Sbjct: 1201 KLLDSSRISQSIFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSW-RL 1256
Query: 1263 HHKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTIVTGKGDIVHAMSAVEDFVYL 1322
+K INS+ VYKD L+S+S+ V+ S ++ RR+ +P+M+I KG + AM VEDF+YL
Sbjct: 1261 QNKPINSVVVYKDMLYSSSTYVEMSNIKDLRRNYEPQMSITAEKGSNIVAMGVVEDFIYL 1256
Query: 1323 ISKSSANSIQIWLRKTQHKVGRISAGSRITCLLTANDMVLCGTETG 1343
SSAN++QIWLR+TQ KVGR+SAGS+IT LLTAND+V CGTE G
Sbjct: 1321 NRSSSANTLQIWLRRTQQKVGRLSAGSKITSLLTANDIVFCGTEAG 1256
BLAST of MS014628 vs. TAIR 10
Match:
AT3G50390.1 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 56.6 bits (135), Expect = 1.8e-07
Identity = 67/279 (24.01%), Postives = 112/279 (40.14%), Query Frame = 0
Query: 1067 NSSGAVCALIFYKGLLFGGYSDGSIKVWNIK----------GQSASLLWDIK-------- 1126
++SG V A++ +F G+ DG I+VW G +LL I+
Sbjct: 134 SNSGLVKAIVLAGDKIFTGHQDGKIRVWKAASKESNVHRRVGTMPNLLDYIRNSIVPSSY 193
Query: 1127 ---------------KHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIES 1186
+H A++C + E L SGS DKT +VW++ L C E + +
Sbjct: 194 FNFTRRNRSSAALGFRHLDAISCLALSEDKRLLYSGSWDKTFKVWRV--SDLRCVESVNA 253
Query: 1187 KEQIQH--LGAYGQIIFAITHGHGLKV---IDATRTTKVLFKSKNLK------CIKVVQA 1246
E + + + ++F + +KV D + TK F LK I V Q+
Sbjct: 254 HEDAVNAVVSGFDGLVFTGSADGTVKVWRREDQAKDTKHFFSETLLKQDCAVTAIAVDQS 313
Query: 1247 R--VYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQG 1293
VY G +D ++ N WE+E + + H A+ L + +FS S+ +
Sbjct: 314 ATLVYCGSSDGTV------NFWERENNMKNGGVLKGHKLAVLCLVAAGNLMFSGSADLG- 373
BLAST of MS014628 vs. TAIR 10
Match:
AT5G49200.1 (WD-40 repeat family protein / zfwd4 protein (ZFWD4) )
HSP 1 Score: 55.1 bits (131), Expect = 5.2e-07
Identity = 36/102 (35.29%), Postives = 50/102 (49.02%), Query Frame = 0
Query: 1070 GAVCALIFYKGLLFGGYSDGSIKVWNIKGQSAS----LLWDIKKHRKAVTCFSHFESGES 1129
G V A+ G+LF G S GSI VW S S L ++ H VTCF+ G+
Sbjct: 213 GQVNAMTIANGMLFAGTSSGSILVWKATTDSESDPFKYLTSLEGHSGEVTCFA--VGGQM 272
Query: 1130 LLSGSTDKTIRVWKMIQGKLECTEVIESKEQIQHLGAYGQII 1168
L SGS DKTI++W + L+C ++ QH G ++
Sbjct: 273 LYSGSVDKTIKMWDL--NTLQCIMTLK-----QHTGTVTSLL 305
BLAST of MS014628 vs. TAIR 10
Match:
AT1G47610.1 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 52.8 bits (125), Expect = 2.6e-06
Identity = 72/301 (23.92%), Postives = 121/301 (40.20%), Query Frame = 0
Query: 1065 GFNS-SGAVCALIFYK-GLLFGGYSDGSIKVWNIKGQ---------SASLLWDIK----- 1124
GF S SG V A++ + +F G+ DG I+VW + S L D+
Sbjct: 57 GFKSNSGLVKAIVISREAKVFTGHQDGKIRVWKTSSKNPRVYTRAGSLPALKDVLKSSVK 116
Query: 1125 ----------------KHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIE 1184
KH AV+C S E L S S D+T++VW++ L+C E I+
Sbjct: 117 PSNYVEVRRCRTALWIKHSDAVSCLSLAEDQGLLYSASWDRTVKVWRI--HDLKCIESIK 176
Query: 1185 SKEQ-IQHLGAYGQIIFAITHGHGLKV----IDATRTTKVLFKS-----KNLKCIKVVQA 1244
+ + + + ++F + +KV I RT LF++ + +
Sbjct: 177 AHDDAVNSVTTAESLVFTGSADGTVKVWKREIRGKRTAHSLFQTLLKQESAVTALVTSHM 236
Query: 1245 RVYAGCTDSSIQEFSVTNKWEQEIKPPSKSWMMM--HHKAINSLAVYKDWLFSASSMVQG 1304
VY+G +D ++ N WE K K + H A+ +A LFS ++ +
Sbjct: 237 AVYSGSSDGAV------NFWEMGDKKLLKHCEVFKKHRLAVLCIAAAGKLLFSGAADKKI 296
Query: 1305 SLFQNWRRHDKPE--MTIVTGKGDIVHAMSAVEDF-----------VYLISKSSANSIQI 1309
+ WRR K ++++TG V ++ VE + L S S S+++
Sbjct: 297 CV---WRREGKVHTCVSVLTGHTGPVKCLAVVEPSGGEEEDGGDGRLVLYSGSLDKSVKV 346
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022137811.1 | 0.0e+00 | 97.99 | putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Momordica charantia] | [more] |
XP_038894856.1 | 0.0e+00 | 84.39 | putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Benincasa hispida] | [more] |
XP_023526729.1 | 0.0e+00 | 83.88 | putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo] >XP_0235... | [more] |
KAG6582207.1 | 0.0e+00 | 83.51 | putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subs... | [more] |
KAG7018606.1 | 0.0e+00 | 83.43 | putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita argyrosperma subsp. argyro... | [more] |
Match Name | E-value | Identity | Description | |
C6L7U1 | 3.9e-76 | 22.86 | Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBER... | [more] |
D1FP53 | 5.3e-73 | 22.84 | Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN P... | [more] |
D1FP57 | 4.9e-71 | 23.06 | Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE... | [more] |
P90648 | 6.4e-10 | 25.74 | Myosin heavy chain kinase B OS=Dictyostelium discoideum OX=44689 GN=mhkB PE=2 SV... | [more] |
Q9NWT1 | 5.0e-07 | 28.00 | p21-activated protein kinase-interacting protein 1 OS=Homo sapiens OX=9606 GN=PA... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C7S7 | 0.0e+00 | 97.99 | putative E3 ubiquitin-protein ligase LIN-1 isoform X1 OS=Momordica charantia OX=... | [more] |
A0A6J1GTU3 | 0.0e+00 | 83.36 | putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
A0A0A0L5U3 | 0.0e+00 | 83.12 | WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... | [more] |
A0A6J1ITY3 | 0.0e+00 | 82.99 | putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
A0A1S3AXG3 | 0.0e+00 | 82.47 | putative E3 ubiquitin-protein ligase LIN-1 OS=Cucumis melo OX=3656 GN=LOC1034838... | [more] |
Match Name | E-value | Identity | Description | |
AT3G06880.2 | 4.3e-296 | 44.49 | Transducin/WD40 repeat-like superfamily protein | [more] |
AT3G06880.1 | 2.8e-295 | 44.44 | Transducin/WD40 repeat-like superfamily protein | [more] |
AT3G50390.1 | 1.8e-07 | 24.01 | Transducin/WD40 repeat-like superfamily protein | [more] |
AT5G49200.1 | 5.2e-07 | 35.29 | WD-40 repeat family protein / zfwd4 protein (ZFWD4) | [more] |
AT1G47610.1 | 2.6e-06 | 23.92 | Transducin/WD40 repeat-like superfamily protein | [more] |