MS014621 (gene) Bitter gourd (TR) v1

Overview
NameMS014621
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAdenine nucleotide alpha hydrolases-like superfamily protein
Locationscaffold1096: 130865 .. 132794 (+)
RNA-Seq ExpressionMS014621
SyntenyMS014621
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCAGGTGATTGTGATTGGCGTGGATGAGAGCGATCACAGCTTCTACGCCTTGCAGTGGATGCTCCAACATTTCGTTGCTCCTATCTCCAATTCTCCTTACAAGCTTGTTATCGTCCACGCTAAACCGCCTCCCTCCAGCGTTATTGCAGTTACCGGCCCAGGTATAGATACTCCCGAACTCTGGTTTTGGATTTGTTTTGCCAATTCGATTGTTCCGTTTGGTTCCTCCGAATGCGAAGATTAATTTGAAACCAGGTTGATTCTTAAAGAAAGGTATCTACCACAGGGGAAATTGAATTTACTTTCTGTACTGCTTGTTTTTGGATATCGAATTGTGATGAAAGAGCATATATATATTTCGAAGCTACGATCTACTCGCTACGTATAATTGAAGCGAATCAAGAAATCTGTATACATTTTGAAGCCCTTTCTTATAATCAAGTTGCGATTTGTAACATTTTCCCCAATGAATATCAAATTTGTGGGCAGTGTCAGTGTGGTTTCTTCCTAACAAATGTTTTGAAATACAGGAGCCGCGAACGCCTTGTCCTTACTAGATGCAGATCTCAATAAAGTAGCTAACAGGACTGTCCAAAAAGCGAAGGATATATGCATCGAGCACAAGGTAAAGTCCATCTGTGATTTATCTGGTTTTTTCTAAACGGTTCTCCAGCTCTTATTTCTCTGATCACTTTCATTTAGAATTTTGATTGCTTAAAAGCTATTTCTCCAAATAGGACCTGAATTGCATTGCTCTTAATTGGCTTCCATTCTGATTAACGATTGAACTACTTGTTTATAAAATCTTATTTATACTTGAATAATGAAACTCATTTCTTTTTATCGTAATTTTACTAAGCCTCAAAATCAGGTCAAATTAATAGTTTGGATAATATTTCTAACTCTTACTCTTCCACATTTGCACAAATAAGCTCTCACAAATGTAATTTGCGGCACCCGAGAAAGTGGGGGTGATTGCTATTCACCATCTGAGTTCATACACAGTTATGCAAATCTCCTTGCCCGTCTATTGAAAACATGACCAATTTGTTTGTCTTTTTTTTTCTTTTTTATATATATATATAAACCATGGGGATGTATAGGAGTAAGAACTGCCCAGAATCCATAGAATAACTTGAACTACTGCCAATTGTACTGTTTTAGGACATGACTGAGTTGAAACTGGAGCCACCATCTGTTCTGCAAAAAAATTTACATAACCATTTAGCAGGCTTATTGAGACAATCTTCTGCCATGCAGATTGAAAACGTACAGGTCGAAATTGTGGAGGGCGATGCCAGAAATGTCATGTGCGATGCTGTAGAAAAATTCCATGCATCCATTTTGGTTGTTGGAAGTCACAACTATGGAGTAGTGAAAAGGTAAAGACTCCCTTACTATAAAAGAAACTGACTGATTTTATTGTGTAGTTCGTAGTTTTGCCTTTATGCTTTCCATAAAACACAACTCAACTGTTGAATGCATCAGTAGTAATAGTTTTGAATTTCCACCTTCTGATTGAATTGTGATACTCCAACAAAACCCTAACTCAAACTAGCATTCCCTAGCGAATCATAAATTAATAAAATATTAAGCTATTAGCTCTAGTGGCAATTTAATATGATACAGAGTCTTGTGTTTCAGTCTTTAATGTCATTTCCTATCCAATTAATACTAGTAACGACTTGTTCGGCTTTGTGCTAATTTCTAAGAAGTTACAAGTGAGAGAAAGTGCTAAGAATATGAGTAAAAGATTAAATTCACCGTAACCTAATATTGCTTAAACTTTTGGATTTAGCAGTGAAATACTAATAAATTTGTTTTTGTTAAACAGGATGGCTCTGGGAAGTGTAAGTAACTACTGTGCTAATCATGCCCATTGCTCTGTCATGATTGTGAAGAGGCCACCCAAGCCCATGAAC

mRNA sequence

ATGGCGGAGCAGGTGATTGTGATTGGCGTGGATGAGAGCGATCACAGCTTCTACGCCTTGCAGTGGATGCTCCAACATTTCGTTGCTCCTATCTCCAATTCTCCTTACAAGCTTGTTATCGTCCACGCTAAACCGCCTCCCTCCAGCGTTATTGCAGTTACCGGCCCAGGAGCCGCGAACGCCTTGTCCTTACTAGATGCAGATCTCAATAAAGTAGCTAACAGGACTGTCCAAAAAGCGAAGGATATATGCATCGAGCACAAGATTGAAAACGTACAGGTCGAAATTGTGGAGGGCGATGCCAGAAATGTCATGTGCGATGCTGTAGAAAAATTCCATGCATCCATTTTGGTTGTTGGAAGTCACAACTATGGAGTAGTGAAAAGGATGGCTCTGGGAAGTGTAAGTAACTACTGTGCTAATCATGCCCATTGCTCTGTCATGATTGTGAAGAGGCCACCCAAGCCCATGAAC

Coding sequence (CDS)

ATGGCGGAGCAGGTGATTGTGATTGGCGTGGATGAGAGCGATCACAGCTTCTACGCCTTGCAGTGGATGCTCCAACATTTCGTTGCTCCTATCTCCAATTCTCCTTACAAGCTTGTTATCGTCCACGCTAAACCGCCTCCCTCCAGCGTTATTGCAGTTACCGGCCCAGGAGCCGCGAACGCCTTGTCCTTACTAGATGCAGATCTCAATAAAGTAGCTAACAGGACTGTCCAAAAAGCGAAGGATATATGCATCGAGCACAAGATTGAAAACGTACAGGTCGAAATTGTGGAGGGCGATGCCAGAAATGTCATGTGCGATGCTGTAGAAAAATTCCATGCATCCATTTTGGTTGTTGGAAGTCACAACTATGGAGTAGTGAAAAGGATGGCTCTGGGAAGTGTAAGTAACTACTGTGCTAATCATGCCCATTGCTCTGTCATGATTGTGAAGAGGCCACCCAAGCCCATGAAC

Protein sequence

MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAANALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVGSHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN
Homology
BLAST of MS014621 vs. NCBI nr
Match: XP_022138083.1 (universal stress protein PHOS34-like [Momordica charantia])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN
Sbjct: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
           ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG
Sbjct: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 159
           SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN
Sbjct: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 158

BLAST of MS014621 vs. NCBI nr
Match: XP_038904684.1 (universal stress protein PHOS34-like isoform X2 [Benincasa hispida])

HSP 1 Score: 252.3 bits (643), Expect = 2.8e-63
Identity = 121/158 (76.58%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MAEQV+VIGVDES+HSFYAL W LQHF  P + +PYKLVIV+AKPPPSS + V GP A +
Sbjct: 1   MAEQVMVIGVDESEHSFYALNWTLQHFFGP-NATPYKLVIVNAKPPPSSFLGVAGPAALD 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L +LDADL K+A+RTVQKAKDICIEHK+++VQ E++EGDAR VMCD+VEKFHASILVVG
Sbjct: 61  VLPMLDADLKKIADRTVQKAKDICIEHKVQDVQTEVLEGDARTVMCDSVEKFHASILVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 159
           SHNYGVVKRM LGSVS++CA+HAHCSVMIVKRPPKPM+
Sbjct: 121 SHNYGVVKRMGLGSVSDFCAHHAHCSVMIVKRPPKPMS 157

BLAST of MS014621 vs. NCBI nr
Match: XP_022973665.1 (universal stress protein PHOS34-like [Cucurbita maxima] >XP_022975168.1 universal stress protein PHOS34-like [Cucurbita maxima])

HSP 1 Score: 251.5 bits (641), Expect = 4.7e-63
Identity = 120/157 (76.43%), Postives = 140/157 (89.17%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MA++V+VIGVDE++HSFYAL+WMLQHF  P ++ P+ LVIVHAKPPPSSV+A++GPGA N
Sbjct: 1   MADKVLVIGVDETEHSFYALRWMLQHFFGP-NDIPHNLVIVHAKPPPSSVLAISGPGAEN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L LLD DL K+ NRTVQ+AK+ICIEHK++NV  E+VEGDARNVMCDAVEK HAS+LVVG
Sbjct: 61  VLPLLDEDLKKIGNRTVQRAKEICIEHKVQNVTTEVVEGDARNVMCDAVEKHHASLLVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           SHNYGVVK M LGSVS YCA+HAHCSVMIVKRPPKPM
Sbjct: 121 SHNYGVVKGMNLGSVSAYCAHHAHCSVMIVKRPPKPM 156

BLAST of MS014621 vs. NCBI nr
Match: XP_022937984.1 (universal stress protein PHOS34-like [Cucurbita moschata] >KAG6597526.1 hypothetical protein SDJN03_10706, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028983.1 hypothetical protein SDJN02_10166 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249.2 bits (635), Expect = 2.3e-62
Identity = 119/157 (75.80%), Postives = 139/157 (88.54%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MA++V+VIGVDE++HSFYAL+WMLQHF  P ++ P+ LVIVHAKPPPSSV+A++GP A N
Sbjct: 1   MADKVLVIGVDETEHSFYALRWMLQHFFGP-NDIPHNLVIVHAKPPPSSVLAISGPAAEN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L LLD DL K+ NRTVQ+AK+ICIEHK++NV  E+VEGDARNVMCDAVEK HAS+LVVG
Sbjct: 61  VLPLLDEDLKKIGNRTVQRAKEICIEHKVQNVTTEVVEGDARNVMCDAVEKHHASLLVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           SHNYGVVK M LGSVS YCA+HAHCSVMIVKRPPKPM
Sbjct: 121 SHNYGVVKGMNLGSVSAYCAHHAHCSVMIVKRPPKPM 156

BLAST of MS014621 vs. NCBI nr
Match: XP_038904675.1 (universal stress protein PHOS34-like isoform X1 [Benincasa hispida])

HSP 1 Score: 247.7 bits (631), Expect = 6.8e-62
Identity = 121/158 (76.58%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MAEQV+VIGVDES+HSFYAL W LQHF  P + +PYKLVIV+AKPPPSS + V GP A +
Sbjct: 1   MAEQVMVIGVDESEHSFYALNWTLQHFFGP-NATPYKLVIVNAKPPPSSFLGVAGP-ALD 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L +LDADL K+A+RTVQKAKDICIEHK+++VQ E++EGDAR VMCD+VEKFHASILVVG
Sbjct: 61  VLPMLDADLKKIADRTVQKAKDICIEHKVQDVQTEVLEGDARTVMCDSVEKFHASILVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 159
           SHNYGVVKRM LGSVS++CA+HAHCSVMIVKRPPKPM+
Sbjct: 121 SHNYGVVKRMGLGSVSDFCAHHAHCSVMIVKRPPKPMS 156

BLAST of MS014621 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.6e-11
Identity = 54/172 (31.40%), Postives = 86/172 (50.00%), Query Frame = 0

Query: 2   AEQVIVIGVDESDHSFYALQWMLQHFVAP------ISNSPYKLVI--------VHAKPPP 61
           A + I + VD S+ S +A++W + H++ P      +  SP  ++         +   PPP
Sbjct: 43  ARRKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSVLFGADWGPLPLQTPPPP 102

Query: 62  SSVIAVTGPGAANALSLLDADL---NKVANRTVQKAKDICIEHKIENVQVEIVEGDARNV 121
           S   A T PGA    S  D D    +KVA+   +  K+    HKI  V+    + D R  
Sbjct: 103 S---AATDPGAQPKPSQEDFDAFTSSKVAD-LAKPLKEAGFPHKIHIVK----DHDMRER 162

Query: 122 MCDAVEKFHASILVVGSHNYGVVKRMA---LGSVSNYCANHAHCSVMIVKRP 154
           +C   E+ + S +++GS  +G  KR +   LGSVS+YC +H  C V++V+ P
Sbjct: 163 LCLETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVRYP 206

BLAST of MS014621 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-08
Identity = 47/169 (27.81%), Postives = 80/169 (47.34%), Query Frame = 0

Query: 2   AEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKP----------PPSSVI 61
           A + I + VD S+ S +A++W + H++ P       +V++H  P          P     
Sbjct: 46  ARRKIGVAVDLSEESSFAVRWAVDHYIRPGD----AVVLLHVSPTSVLFGADWGPLPLKT 105

Query: 62  AVTGPGAANALSLLDADL---NKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDA 121
            +  P A    S  D D     KVA+   +  K++   +KI  V+    + D R  +C  
Sbjct: 106 QIEDPNAQPQPSQEDFDAFTSTKVAD-LAKPLKELGFPYKIHIVK----DHDMRERLCLE 165

Query: 122 VEKFHASILVVGSHNYGVVKRMA----LGSVSNYCANHAHCSVMIVKRP 154
           +E+   S +++GS  +G  K+      LGSVS+YC +H  C V++V+ P
Sbjct: 166 IERLGLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVRYP 205

BLAST of MS014621 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.2e-06
Identity = 38/142 (26.76%), Postives = 67/142 (47.18%), Query Frame = 0

Query: 19  ALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAANALSLLDADLNKVANRTVQ 78
           A +W L+  V   + S +K++++H +     V+   G    +++     D   +  R   
Sbjct: 30  AFEWTLEKIVRS-NTSDFKILLLHVQ-----VVDEDGFDDVDSIYASPEDFRDM--RQSN 89

Query: 79  KAKDICIEHKIEN--------VQVEIVEGDARNVMCDAVEKFHASILVVGSHNYGVVKRM 138
           KAK + +     N         +  I  GD ++V+C  V++     LVVGS   G  +++
Sbjct: 90  KAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGRFQKV 149

Query: 139 ALGSVSNYCANHAHCSVMIVKR 153
            +G+VS +C  HA C VM +KR
Sbjct: 150 FVGTVSAFCVKHAECPVMTIKR 163

BLAST of MS014621 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 5.5e-06
Identity = 40/150 (26.67%), Postives = 70/150 (46.67%), Query Frame = 0

Query: 6   IVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAANALSLL 65
           IVI  D SD S  A +  + +         Y + +V   P         G  A  +  L+
Sbjct: 29  IVIPTDGSDVSLEAAKHAI-NIAKEFDAEVYAIYVVDVSP-------FVGLPAEGSWELI 88

Query: 66  DADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVGSHNYG 125
              L +     ++K K +  E  ++ +  E++EG   N + +  EK  A ++V+G+    
Sbjct: 89  SELLKEEGQEALKKVKKMAEEWGVK-IHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKT 148

Query: 126 VVKRMALGSVSNYCANHAHCSVMIVKRPPK 156
            ++R+ LGSV+     +AHC V++VK+P K
Sbjct: 149 GLERILLGSVAERVIKNAHCPVLVVKKPKK 169

BLAST of MS014621 vs. ExPASy TrEMBL
Match: A0A6J1CC29 (universal stress protein PHOS34-like OS=Momordica charantia OX=3673 GN=LOC111009340 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.8e-83
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN
Sbjct: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
           ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG
Sbjct: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 159
           SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN
Sbjct: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPMN 158

BLAST of MS014621 vs. ExPASy TrEMBL
Match: A0A6J1IBY1 (universal stress protein PHOS34-like OS=Cucurbita maxima OX=3661 GN=LOC111472250 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.3e-63
Identity = 120/157 (76.43%), Postives = 140/157 (89.17%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MA++V+VIGVDE++HSFYAL+WMLQHF  P ++ P+ LVIVHAKPPPSSV+A++GPGA N
Sbjct: 1   MADKVLVIGVDETEHSFYALRWMLQHFFGP-NDIPHNLVIVHAKPPPSSVLAISGPGAEN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L LLD DL K+ NRTVQ+AK+ICIEHK++NV  E+VEGDARNVMCDAVEK HAS+LVVG
Sbjct: 61  VLPLLDEDLKKIGNRTVQRAKEICIEHKVQNVTTEVVEGDARNVMCDAVEKHHASLLVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           SHNYGVVK M LGSVS YCA+HAHCSVMIVKRPPKPM
Sbjct: 121 SHNYGVVKGMNLGSVSAYCAHHAHCSVMIVKRPPKPM 156

BLAST of MS014621 vs. ExPASy TrEMBL
Match: A0A6J1FCR8 (universal stress protein PHOS34-like OS=Cucurbita moschata OX=3662 GN=LOC111444203 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 119/157 (75.80%), Postives = 139/157 (88.54%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MA++V+VIGVDE++HSFYAL+WMLQHF  P ++ P+ LVIVHAKPPPSSV+A++GP A N
Sbjct: 1   MADKVLVIGVDETEHSFYALRWMLQHFFGP-NDIPHNLVIVHAKPPPSSVLAISGPAAEN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            L LLD DL K+ NRTVQ+AK+ICIEHK++NV  E+VEGDARNVMCDAVEK HAS+LVVG
Sbjct: 61  VLPLLDEDLKKIGNRTVQRAKEICIEHKVQNVTTEVVEGDARNVMCDAVEKHHASLLVVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           SHNYGVVK M LGSVS YCA+HAHCSVMIVKRPPKPM
Sbjct: 121 SHNYGVVKGMNLGSVSAYCAHHAHCSVMIVKRPPKPM 156

BLAST of MS014621 vs. ExPASy TrEMBL
Match: A0A0A0LAL5 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G154380 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.8e-53
Identity = 108/157 (68.79%), Postives = 129/157 (82.17%), Query Frame = 0

Query: 1   MAEQVIVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVTGPGAAN 60
           MAEQV+VIGVDES+HSFYAL W LQHF  P + +PYKL IV+A   PS        G+ N
Sbjct: 1   MAEQVMVIGVDESEHSFYALDWTLQHFFRP-NATPYKLTIVNA-TLPSIPHGAAFLGSPN 60

Query: 61  ALSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVG 120
            +  +DADL K+ NRTVQ+AKDICIEH +++V+ E+VEGDARNV+CD+VEKFHASIL+VG
Sbjct: 61  LMPTIDADLKKLTNRTVQRAKDICIEHNVQSVETEVVEGDARNVLCDSVEKFHASILIVG 120

Query: 121 SHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           SH+YGVVK+M LGSVS+YCA HAHCSVMIVKRPPKPM
Sbjct: 121 SHDYGVVKKMGLGSVSDYCAQHAHCSVMIVKRPPKPM 155

BLAST of MS014621 vs. ExPASy TrEMBL
Match: A0A5B7BXB4 (Usp domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_042535 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 5.9e-51
Identity = 99/152 (65.13%), Postives = 122/152 (80.26%), Query Frame = 0

Query: 3   EQVIVIGVDESDHSFYALQWMLQHFVAPI-SNSPYKLVIVHAKPPPSSVIAVTGPGAANA 62
           + V+V+GVDES+HSFYAL+W L HF  P  SNSP+ LVIVHAKP PSSV+ + GPGAA  
Sbjct: 6   KSVMVVGVDESEHSFYALEWTLDHFFTPFASNSPFSLVIVHAKPSPSSVVGLAGPGAAEV 65

Query: 63  LSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVGS 122
              +D+DL K+A R ++KAK+ICI   + +V VE+ EGDARNV+C+AVEK HASILV+GS
Sbjct: 66  FPFVDSDLKKIAARVIEKAKEICISKSVNDVIVEVGEGDARNVLCEAVEKHHASILVLGS 125

Query: 123 HNYGVVKRMALGSVSNYCANHAHCSVMIVKRP 154
           H YG +KR  LGSVS+YCA+HAHCSVMIVKRP
Sbjct: 126 HGYGAIKRAVLGSVSDYCAHHAHCSVMIVKRP 157

BLAST of MS014621 vs. TAIR 10
Match: AT2G47710.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.4e-44
Identity = 83/152 (54.61%), Postives = 108/152 (71.05%), Query Frame = 0

Query: 3   EQVIVIGVDESDHSFYALQWMLQHFVAPIS-NSPYKLVIVHAKPPPSSVIAVTGPGAANA 62
           + V+V+GVD+S+ S YAL+W L  F AP + N P+KL IVHAKP   S + + GPG A  
Sbjct: 7   KSVMVVGVDDSEQSTYALEWTLDRFFAPYAPNYPFKLFIVHAKPNAVSAVGLAGPGTAEV 66

Query: 63  LSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHASILVVGS 122
           +  +DADL   A + V+KAK IC    +    +E+ EGDARN++C+ V+K HASILVVGS
Sbjct: 67  VPYVDADLKHTAAKVVEKAKAICQSRSVHGAVIEVFEGDARNILCEVVDKHHASILVVGS 126

Query: 123 HNYGVVKRMALGSVSNYCANHAHCSVMIVKRP 154
           H YG +KR  LGS S+YCA+HAHCSVMIVK+P
Sbjct: 127 HGYGAIKRAVLGSTSDYCAHHAHCSVMIVKKP 158

BLAST of MS014621 vs. TAIR 10
Match: AT1G09740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.2e-24
Identity = 59/156 (37.82%), Postives = 89/156 (57.05%), Query Frame = 0

Query: 6   IVIGVDESDHSFYALQWMLQHFVAPISNSPYKLVIVHAKPPPSSVIAVT-------GPGA 65
           +V+ VD S+ S  AL+W L +     S+S    V++H +P PS    V+       GP  
Sbjct: 10  VVVAVDGSEVSMEALRWALDNLKLSSSSSDSSFVVLHVQPSPSVAAGVSPGTIPFGGPSG 69

Query: 66  ANA---LSLLDADLNKVANRTVQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKFHAS 125
                  + ++    ++ +  ++ A  IC E  + NV+ ++V GD +  +C+AVE  HA 
Sbjct: 70  LEVPAFTAAIEQHQKRITDTILEHASQICAEKSV-NVKTQVVIGDPKYKICEAVENLHAD 129

Query: 126 ILVVGSHNYGVVKRMALGSVSNYCANHAHCSVMIVK 152
           +LV+GS  YG +KRM LGSVSNYC NHAHC V+I+K
Sbjct: 130 LLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIK 164

BLAST of MS014621 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.1e-24
Identity = 65/166 (39.16%), Postives = 96/166 (57.83%), Query Frame = 0

Query: 6   IVIGVDESDHSFYALQWMLQHF------VAPISNSPYKLVIVHAKPPPSSVIAV-TGPGA 65
           +V+ +DESD SFYALQW++ HF       A        L ++H + P +   A   GPG 
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGPGG 94

Query: 66  ANAL---SLLDADLNKVANRT----VQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEK 125
           A A+   S +   + K    T    + +A  +C   +I   +  ++EG+A+ ++C+AVEK
Sbjct: 95  ATAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQI-RTETLVLEGEAKEMICEAVEK 154

Query: 126 FHASILVVGSHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
            H  +LVVGS   G +KR  LGSVS+YCA+HA+C ++IVK PPK M
Sbjct: 155 MHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK-PPKEM 198

BLAST of MS014621 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 3.5e-24
Identity = 65/165 (39.39%), Postives = 95/165 (57.58%), Query Frame = 0

Query: 6   IVIGVDESDHSFYALQWMLQHF------VAPISNSPYKLVIVHAKPPPSSVIAV-TGPGA 65
           +V+ +DESD SFYALQW++ HF       A        L ++H + P +   A   GPG 
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGPGG 94

Query: 66  AN--ALSLLDADLNKVANRT----VQKAKDICIEHKIENVQVEIVEGDARNVMCDAVEKF 125
           A   A S +   + K    T    + +A  +C   +I   +  ++EG+A+ ++C+AVEK 
Sbjct: 95  ATVYASSSMIESVKKAQQETSAALLSRALQMCRAKQI-RTETLVLEGEAKEMICEAVEKM 154

Query: 126 HASILVVGSHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
           H  +LVVGS   G +KR  LGSVS+YCA+HA+C ++IVK PPK M
Sbjct: 155 HVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK-PPKEM 197

BLAST of MS014621 vs. TAIR 10
Match: AT3G11930.4 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 3.5e-24
Identity = 60/166 (36.14%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 6   IVIGVDESDHSFYALQWMLQHF------VAPISNSPYKLVIVHAKPPPSSVIAV-TGPGA 65
           +V+ +DESD SFYALQW++ HF       A        L ++H + P +   A   GPG 
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGPGG 94

Query: 66  ANALSLLDADLNKVANRTVQKAKDICIEHKIE-------NVQVEIVEGDARNVMCDAVEK 125
           A A     + + +   +  Q+     +   ++         +  ++EG+A+ ++C+AVEK
Sbjct: 95  ATAAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICEAVEK 154

Query: 126 FHASILVVGSHNYGVVKRMALGSVSNYCANHAHCSVMIVKRPPKPM 158
            H  +LVVGS   G +KR  LGSVS+YCA+HA+C ++IVK PPK M
Sbjct: 155 MHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK-PPKEM 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138083.11.2e-82100.00universal stress protein PHOS34-like [Momordica charantia][more]
XP_038904684.12.8e-6376.58universal stress protein PHOS34-like isoform X2 [Benincasa hispida][more]
XP_022973665.14.7e-6376.43universal stress protein PHOS34-like [Cucurbita maxima] >XP_022975168.1 universa... [more]
XP_022937984.12.3e-6275.80universal stress protein PHOS34-like [Cucurbita moschata] >KAG6597526.1 hypothet... [more]
XP_038904675.16.8e-6276.58universal stress protein PHOS34-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8L4N12.6e-1131.40Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S... [more]
Q8VYN92.0e-0827.81Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S... [more]
Q8LGG83.2e-0626.76Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0... [more]
Q579515.5e-0626.67Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43... [more]
Match NameE-valueIdentityDescription
A0A6J1CC295.8e-83100.00universal stress protein PHOS34-like OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A6J1IBY12.3e-6376.43universal stress protein PHOS34-like OS=Cucurbita maxima OX=3661 GN=LOC111472250... [more]
A0A6J1FCR81.1e-6275.80universal stress protein PHOS34-like OS=Cucurbita moschata OX=3662 GN=LOC1114442... [more]
A0A0A0LAL52.8e-5368.79Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G154380 PE=4 SV... [more]
A0A5B7BXB45.9e-5165.13Usp domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_042535 PE=4... [more]
Match NameE-valueIdentityDescription
AT2G47710.12.4e-4454.61Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT1G09740.11.2e-2437.82Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.22.1e-2439.16Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.13.5e-2439.39Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.43.5e-2436.14Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 111..123
score: 32.0
coord: 3..21
score: 25.89
coord: 129..151
score: 37.39
IPR006016UspAPFAMPF00582Uspcoord: 5..151
e-value: 2.2E-24
score: 86.6
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 1..158
e-value: 1.1E-37
score: 131.9
NoneNo IPR availablePANTHERPTHR46553:SF3ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEINcoord: 3..155
NoneNo IPR availablePANTHERPTHR46553FAMILY NOT NAMEDcoord: 3..155
NoneNo IPR availableCDDcd00293USP_Likecoord: 6..150
e-value: 5.6734E-23
score: 86.2698
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 6..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014621.1MS014621.1mRNA