MS014539 (gene) Bitter gourd (TR) v1

BLAST of MS014539 vs. NCBI nr
Match: XP_022155035.1 (RHOMBOID-like protein 9, chloroplastic [Momordica charantia])

HSP 1 Score: 863.2 bits (2229), Expect = 9.7e-247
Identity = 434/446 (97.31%), Postives = 435/446 (97.53%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLKNPPSILNRKQWQEISSYGL 60
           MAVVPTIYYARYHNDSINP QIIFG NKLAYNVC CSPVLKNPPSILNRKQW EISSYG 
Sbjct: 1   MAVVPTIYYARYHNDSINPIQIIFGSNKLAYNVCACSPVLKNPPSILNRKQWPEISSYGP 60

Query: 61  SAVSIAAGTRKSRRGFEPHAWECSSTNEKQLRSSGSYFGRLKGGGNKRKFDSLEKIEVLD 120
           SA+SI A TRKSRRGFEPHAWECSSTNEKQLRS GSYFGRLKGGGNKRKFDSLEKIEVLD
Sbjct: 61  SAISITAETRKSRRGFEPHAWECSSTNEKQLRSLGSYFGRLKGGGNKRKFDSLEKIEVLD 120

Query: 121 TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH 180
           TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH
Sbjct: 121 TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH 180

Query: 181 ERFNDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN 240
           ER NDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN
Sbjct: 181 ERLNDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN 240

Query: 241 ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF 300
           ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF
Sbjct: 241 ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF 300

Query: 301 TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI 360
           TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI
Sbjct: 301 TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI 360

Query: 361 GPIDEWTHTGAAFSGMVYGFLTSPIVEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSF 420
           GPIDEWTHTGAAFSGMVYGFLT PIVEVDD SSSSSPSSRRGQQEGIKLVRKYADPCKSF
Sbjct: 361 GPIDEWTHTGAAFSGMVYGFLTPPIVEVDD-SSSSSPSSRRGQQEGIKLVRKYADPCKSF 420

Query: 421 AFFALFILVFFSLPFFIQPPPTTFPL 447
           AFFALFILVFFSLPFFIQPPPTTFPL
Sbjct: 421 AFFALFILVFFSLPFFIQPPPTTFPL 445

BLAST of MS014539 vs. NCBI nr
Match: XP_038883181.1 (RHOMBOID-like protein 9, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 592.4 bits (1526), Expect = 3.2e-165
Identity = 325/475 (68.42%), Postives = 364/475 (76.63%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLKN--PPSILNRKQWQEISSY 60
           MAVVPTI+  R  NDSINPTQII  PN    ++C+C  V KN  P    ++ +  EI + 
Sbjct: 1   MAVVPTIFQIRNFNDSINPTQIISKPNYKRASICKCDVVSKNNLPIFEYSKNRHWEIQNN 60

Query: 61  GLSAVSIAAGTRKSRR----------------GFE-------PHAWECSSTNEKQLRSSG 120
             S ++IAA   KSRR                 FE          WEC STNEKQLRS G
Sbjct: 61  DSSVITIAARATKSRRRLKAMFLQHVRCSSGLDFESQVLKKLSQVWEC-STNEKQLRSLG 120

Query: 121 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFD 180
           SYFGRL+G GNK+  DSL K +VLDTGQF AKKEL+LLD YF+KV  D+DL NH LS+FD
Sbjct: 121 SYFGRLQGVGNKKNLDSLNKRKVLDTGQFKAKKELELLDAYFQKV--DEDLQNHHLSSFD 180

Query: 181 EQNEGKLFFTSSPFEDQ---EENTPIRHERFNDTI------STTVQEDEISDLYLISSLV 240
           E  E KL FTSSPFED+   +    IRH + N  I      +  +QE+++ DLYLIS+LV
Sbjct: 181 ELQEDKLVFTSSPFEDRNKDDNEMHIRHVKLNGMIHRSMPKTAQLQEEDVCDLYLISALV 240

Query: 241 SVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGL 300
           S+NIAV LFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV L
Sbjct: 241 SINIAVLLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVAL 300

Query: 301 SCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLS 360
           SCW L+TFGRQVCREYGPFTFFLIYVLGGVSGN TSFLHTP+PTVGGTGPVFAMIGAWLS
Sbjct: 301 SCWTLVTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLS 360

Query: 361 YQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIV 420
           YQFQ+K V+ KDV DNMFLKAIIAAVISSILSNIGPIDEW+HTGAAFSGM+YGFLTSPIV
Sbjct: 361 YQFQDKDVVTKDVSDNMFLKAIIAAVISSILSNIGPIDEWSHTGAAFSGMLYGFLTSPIV 420

Query: 421 EVDD-SSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           EV+D +SSSSS SSR+GQ +GIKLVRKYA PCKS A FALFI+ F SL FFIQPP
Sbjct: 421 EVNDAASSSSSSSSRKGQHKGIKLVRKYAHPCKSLAVFALFIMGFTSLVFFIQPP 472

BLAST of MS014539 vs. NCBI nr
Match: XP_038883174.1 (RHOMBOID-like protein 9, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 591.7 bits (1524), Expect = 5.4e-165
Identity = 325/476 (68.28%), Postives = 364/476 (76.47%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLKN--PPSILNRKQWQEISSY 60
           MAVVPTI+  R  NDSINPTQII  PN    ++C+C  V KN  P    ++ +  EI + 
Sbjct: 1   MAVVPTIFQIRNFNDSINPTQIISKPNYKRASICKCDVVSKNNLPIFEYSKNRHWEIQNN 60

Query: 61  GLSAVSIAAGTRKSRR----------------GFE-------PHAWECSSTNEKQLRSSG 120
             S ++IAA   KSRR                 FE          WEC STNEKQLRS G
Sbjct: 61  DSSVITIAARATKSRRRLKAMFLQHVRCSSGLDFESQVLKKLSQVWEC-STNEKQLRSLG 120

Query: 121 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDK-DKDLHNHTLSAF 180
           SYFGRL+G GNK+  DSL K +VLDTGQF AKKEL+LLD YF+KVD+   DL NH LS+F
Sbjct: 121 SYFGRLQGVGNKKNLDSLNKRKVLDTGQFKAKKELELLDAYFQKVDEAHTDLQNHHLSSF 180

Query: 181 DEQNEGKLFFTSSPFEDQ---EENTPIRHERFNDTI------STTVQEDEISDLYLISSL 240
           DE  E KL FTSSPFED+   +    IRH + N  I      +  +QE+++ DLYLIS+L
Sbjct: 181 DELQEDKLVFTSSPFEDRNKDDNEMHIRHVKLNGMIHRSMPKTAQLQEEDVCDLYLISAL 240

Query: 241 VSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVG 300
           VS+NIAV LFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV 
Sbjct: 241 VSINIAVLLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVA 300

Query: 301 LSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWL 360
           LSCW L+TFGRQVCREYGPFTFFLIYVLGGVSGN TSFLHTP+PTVGGTGPVFAMIGAWL
Sbjct: 301 LSCWTLVTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWL 360

Query: 361 SYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPI 420
           SYQFQ+K V+ KDV DNMFLKAIIAAVISSILSNIGPIDEW+HTGAAFSGM+YGFLTSPI
Sbjct: 361 SYQFQDKDVVTKDVSDNMFLKAIIAAVISSILSNIGPIDEWSHTGAAFSGMLYGFLTSPI 420

Query: 421 VEVDD-SSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           VEV+D +SSSSS SSR+GQ +GIKLVRKYA PCKS A FALFI+ F SL FFIQPP
Sbjct: 421 VEVNDAASSSSSSSSRKGQHKGIKLVRKYAHPCKSLAVFALFIMGFTSLVFFIQPP 475

BLAST of MS014539 vs. NCBI nr
Match: XP_004137292.3 (RHOMBOID-like protein 9, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 578.2 bits (1489), Expect = 6.2e-161
Identity = 315/475 (66.32%), Postives = 362/475 (76.21%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLK--NPPSIL--NRKQWQEIS 60
           MAVVPT +  R  N++I+       PNK    VC+   V+   N P+ +  ++    EI 
Sbjct: 37  MAVVPTFFQIRNFNETISK------PNKRVC-VCKWDVVVSKDNLPTFVSYSKHHTWEIH 96

Query: 61  SYGLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRS 120
               S V+I+   RKSRRG +                          WEC STNEKQLRS
Sbjct: 97  DNDSSVVTISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWEC-STNEKQLRS 156

Query: 121 SGSYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSA 180
            GSYFGRL+G G+++  DSL+K++VLDTGQF AKKELQLLD YF+KVDKD  LH H LS+
Sbjct: 157 LGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSS 216

Query: 181 FDEQNEGKLFFTSSPFED--QEENTPIRHERFNDTI------STTVQEDEISDLYLISSL 240
           FDEQ EGKL FT+SP ED  +++   IRH + +  I       T +QE+E+ DLYLIS+L
Sbjct: 217 FDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISAL 276

Query: 241 VSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVG 300
           VS+NIAVFLFE+ASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV 
Sbjct: 277 VSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVA 336

Query: 301 LSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWL 360
           LSCW LLTFGRQVCREYGPFTFFLIYVLGGVSGN TSFLHTP+PTVGGTGPVFAMIGAWL
Sbjct: 337 LSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWL 396

Query: 361 SYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPI 420
           SYQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+YGFLTSPI
Sbjct: 397 SYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPI 456

Query: 421 VEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           VEV+D+SSSS  SSRRGQ++GIKLVRKYA+PC+S AFF LFI+ F SL FFIQPP
Sbjct: 457 VEVNDASSSS--SSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 501

BLAST of MS014539 vs. NCBI nr
Match: XP_008455584.1 (PREDICTED: RHOMBOID-like protein 9, chloroplastic isoform X5 [Cucumis melo])

HSP 1 Score: 578.2 bits (1489), Expect = 6.2e-161
Identity = 311/474 (65.61%), Postives = 359/474 (75.74%), Query Frame = 0

Query: 4   VPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVL-KNPPSIL----NRKQWQEISSY 63
           VPT +  R  ND +NPTQ I  PNK    +C+C  V+ KN   IL        W EI + 
Sbjct: 3   VPTFFQIRNFNDLLNPTQTISKPNKRVC-ICKCDVVVSKNNLPILVTYSKHHNW-EIHNN 62

Query: 64  GLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRSSG 123
             S V I+   RKS  G +                          W+C +TNEKQLRS G
Sbjct: 63  DSSVVKISTRARKSIGGLKALLQHVRCSNGLNFEYSKVLEKLSQVWKC-TTNEKQLRSLG 122

Query: 124 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFD 183
           SYFGRL+G G+K+  DSL+K++VLDTGQF AKKELQLLD YF+KVDKD DLH H LS+FD
Sbjct: 123 SYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPLSSFD 182

Query: 184 EQNEGKLFFTSSPFED---QEENTPIRHERFNDTI------STTVQEDEISDLYLISSLV 243
           +Q EGKL FT+SPFED    ++ + I+H + +         +T +QE+E+ DLYLIS+LV
Sbjct: 183 DQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYLISALV 242

Query: 244 SVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGL 303
           S+NIAVFLFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV L
Sbjct: 243 SINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVAL 302

Query: 304 SCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLS 363
           SCW LLTFGRQVCR+YGPFTFFLIYVLGGVSGN TSFLHTP PTVGGTGPVFAMIGAWLS
Sbjct: 303 SCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAMIGAWLS 362

Query: 364 YQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIV 423
           YQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+YGFLTSP+ 
Sbjct: 363 YQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPLG 422

Query: 424 EVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           +V+D+SS    SSRRGQ++GIK+VRKYA+PCKSFAFF LFI+ F SL FF+QPP
Sbjct: 423 KVNDASS----SSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPP 469

BLAST of MS014539 vs. ExPASy Swiss-Prot
Match: Q9FFX0 (RHOMBOID-like protein 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL9 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.4e-75
Identity = 162/362 (44.75%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 88  EKQLRSSGSYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLH 147
           +++L+   SYFG+L+    K    + + I+     +    +EL  L  Y +K+ KD    
Sbjct: 77  KQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDSLSAYLDKLQKDAKSK 136

Query: 148 NHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRHERFNDTISTTVQEDEISDLYLISSLVS 207
               S  D          S   +   EN     ++F+D      Q ++  + Y +S L S
Sbjct: 137 GLVSSTLDVVKSEGGSVASKLRKTGIENNNSPFQQFDD----EDQAEDTLNFYAVSILAS 196

Query: 208 VNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGLS 267
           +N+ V LFE A+PV+N+ + L SLP +YGAKIN+LIL GEWWRLVTPMFLHSG+ HV LS
Sbjct: 197 INVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLILAGEWWRLVTPMFLHSGIPHVALS 256

Query: 268 CWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLSY 327
            WALLTFG +VCR+YG FTF LIY+LGGVSGNF SFLHT +PTVGGTGP FA+IGAWL  
Sbjct: 257 SWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSFLHTADPTVGGTGPAFALIGAWLVD 316

Query: 328 QFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIVE 387
           Q QNK +I  +  +++F KAII      ILS+ GPID+WT+ GA  +G+VYGF T P+++
Sbjct: 317 QNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPIDDWTNLGALIAGIVYGFFTCPVLQ 376

Query: 388 VDDSSSSSSPSSRRGQQEGIKLV----RKYADPCKSFAFFALFILVFFSLPFFIQPPPTT 446
           +    S         +QEGI  V    +  ADPCKSF  F +F+ V  +    I   P  
Sbjct: 377 LGSGGSE--------RQEGIVTVGPEKQNSADPCKSFLLFTIFVAVIVTSLLLIGDGPLD 424

BLAST of MS014539 vs. ExPASy Swiss-Prot
Match: P54493 (Rhomboid protease GluP OS=Bacillus subtilis (strain 168) OX=224308 GN=gluP PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 2.2e-20
Identity = 69/212 (32.55%), Postives = 103/212 (48.58%), Query Frame = 0

Query: 178 IRHERFNDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGA 237
           ++ E    T +   Q  + +  YL  +L    +  FL EI     N+E  +      +GA
Sbjct: 159 LKQEEQRKTEAAVFQNGKPTFTYLFIALQI--LMFFLLEINGGSTNTETLV-----AFGA 218

Query: 238 KINELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVS 297
           K N LI  GEWWRL+TP+ LH G+ H+  +  AL + G  V R YG   F LIY+  G++
Sbjct: 219 KENSLIAQGEWWRLLTPIVLHIGIAHLAFNTLALWSVGTAVERMYGSGRFLLIYLAAGIT 278

Query: 298 GNFTSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSIL 357
           G+  SF+ +P P+ G +G +F  +GA L     N+          MFL+ I   +I  I+
Sbjct: 279 GSIASFVFSPYPSAGASGAIFGCLGALLYVALSNR---------KMFLRTIGTNIIVIII 338

Query: 358 SNIG------PIDEWTHTGAAFSGMVYGFLTS 384
            N+G       ID   H G    G++ GF  +
Sbjct: 339 INLGFGFAVSNIDNSGHIG----GLIGGFFAA 350

BLAST of MS014539 vs. ExPASy Swiss-Prot
Match: F4ICF4 (RHOMBOID-like protein 10, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL10 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-13
Identity = 52/187 (27.81%), Postives = 90/187 (48.13%), Query Frame = 0

Query: 205 LVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHV 264
           L+++N+ +++ +IAS  K            +GAKIN LI  G+ WRL T   LH+   H+
Sbjct: 133 LLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPMHL 192

Query: 265 GLSCWALLTFGRQVCREYGPFTFFLIYVLGGVS-------GNFTSFLHTPEPTVGGTGPV 324
            ++C++L + G       GP  F  +Y+   V+       G+  S+     P+VG +G +
Sbjct: 193 MINCYSLNSIGPTAESLGGPKRFLAVYLTSAVAKPILRVLGSAMSYWFNKAPSVGASGAI 252

Query: 325 FAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMV 384
           F ++G+   +  ++K ++     D M +  IIA  ++  L +   ID W H G    G  
Sbjct: 253 FGLVGSVAVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGGTA 309

BLAST of MS014539 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-11
Identity = 44/153 (28.76%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 230 SLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFL 289
           +L ++ G  + +++   E WRL++  +LH GV H+ ++   LL  G ++ RE+G     L
Sbjct: 121 TLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGL 180

Query: 290 IYVLGGVSGNFTSFLH-TPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAI 349
           +Y++ G  G+  S L      +VG +G VF ++G  LS  F N  + +  V+  + L  I
Sbjct: 181 LYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVTLVLI 240

Query: 350 IAAVISSILSNIGPIDEWTHTGAAFSGMVYGFL 382
           +A  +   L  +  +D + H G   +G + GF+
Sbjct: 241 VAVNLG--LGVLPGVDNFAHIGGFATGFLLGFV 271

BLAST of MS014539 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.2e-11
Identity = 51/188 (27.13%), Postives = 89/188 (47.34%), Query Frame = 0

Query: 247 EWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHT 306
           E WRL+T M+LH+G+ H+ ++ + ++ FG ++ +++G     LIY++ G  G+  S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 307 PEP-TVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDE 366
            +  +VG +G +  ++GA LS    N  +    +   +    IIA  I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFLFIIA--INLAIGLLPWVDN 215

Query: 367 WTHTGAAFSGMVYGFLTSPIVEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFAL 426
           + H G   +G   GF+            S     R   Q G +   KY +PC+   FF  
Sbjct: 216 FAHIGGLLTGFCLGFILLM------QPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVA 274

Query: 427 FILVFFSL 434
            +LV   L
Sbjct: 276 AVLVVAGL 274

BLAST of MS014539 vs. ExPASy TrEMBL
Match: A0A6J1DLA4 (RHOMBOID-like protein 9, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022178 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 4.7e-247
Identity = 434/446 (97.31%), Postives = 435/446 (97.53%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLKNPPSILNRKQWQEISSYGL 60
           MAVVPTIYYARYHNDSINP QIIFG NKLAYNVC CSPVLKNPPSILNRKQW EISSYG 
Sbjct: 1   MAVVPTIYYARYHNDSINPIQIIFGSNKLAYNVCACSPVLKNPPSILNRKQWPEISSYGP 60

Query: 61  SAVSIAAGTRKSRRGFEPHAWECSSTNEKQLRSSGSYFGRLKGGGNKRKFDSLEKIEVLD 120
           SA+SI A TRKSRRGFEPHAWECSSTNEKQLRS GSYFGRLKGGGNKRKFDSLEKIEVLD
Sbjct: 61  SAISITAETRKSRRGFEPHAWECSSTNEKQLRSLGSYFGRLKGGGNKRKFDSLEKIEVLD 120

Query: 121 TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH 180
           TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH
Sbjct: 121 TGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRH 180

Query: 181 ERFNDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN 240
           ER NDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN
Sbjct: 181 ERLNDTISTTVQEDEISDLYLISSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKIN 240

Query: 241 ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF 300
           ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF
Sbjct: 241 ELILVGEWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNF 300

Query: 301 TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI 360
           TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI
Sbjct: 301 TSFLHTPEPTVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNI 360

Query: 361 GPIDEWTHTGAAFSGMVYGFLTSPIVEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSF 420
           GPIDEWTHTGAAFSGMVYGFLT PIVEVDD SSSSSPSSRRGQQEGIKLVRKYADPCKSF
Sbjct: 361 GPIDEWTHTGAAFSGMVYGFLTPPIVEVDD-SSSSSPSSRRGQQEGIKLVRKYADPCKSF 420

Query: 421 AFFALFILVFFSLPFFIQPPPTTFPL 447
           AFFALFILVFFSLPFFIQPPPTTFPL
Sbjct: 421 AFFALFILVFFSLPFFIQPPPTTFPL 445

BLAST of MS014539 vs. ExPASy TrEMBL
Match: A0A1S3C0U5 (RHOMBOID-like protein 9, chloroplastic isoform X5 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 3.0e-161
Identity = 311/474 (65.61%), Postives = 359/474 (75.74%), Query Frame = 0

Query: 4   VPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVL-KNPPSIL----NRKQWQEISSY 63
           VPT +  R  ND +NPTQ I  PNK    +C+C  V+ KN   IL        W EI + 
Sbjct: 3   VPTFFQIRNFNDLLNPTQTISKPNKRVC-ICKCDVVVSKNNLPILVTYSKHHNW-EIHNN 62

Query: 64  GLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRSSG 123
             S V I+   RKS  G +                          W+C +TNEKQLRS G
Sbjct: 63  DSSVVKISTRARKSIGGLKALLQHVRCSNGLNFEYSKVLEKLSQVWKC-TTNEKQLRSLG 122

Query: 124 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFD 183
           SYFGRL+G G+K+  DSL+K++VLDTGQF AKKELQLLD YF+KVDKD DLH H LS+FD
Sbjct: 123 SYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPLSSFD 182

Query: 184 EQNEGKLFFTSSPFED---QEENTPIRHERFNDTI------STTVQEDEISDLYLISSLV 243
           +Q EGKL FT+SPFED    ++ + I+H + +         +T +QE+E+ DLYLIS+LV
Sbjct: 183 DQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYLISALV 242

Query: 244 SVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGL 303
           S+NIAVFLFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV L
Sbjct: 243 SINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVAL 302

Query: 304 SCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLS 363
           SCW LLTFGRQVCR+YGPFTFFLIYVLGGVSGN TSFLHTP PTVGGTGPVFAMIGAWLS
Sbjct: 303 SCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAMIGAWLS 362

Query: 364 YQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIV 423
           YQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+YGFLTSP+ 
Sbjct: 363 YQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPLG 422

Query: 424 EVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           +V+D+SS    SSRRGQ++GIK+VRKYA+PCKSFAFF LFI+ F SL FF+QPP
Sbjct: 423 KVNDASS----SSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPP 469

BLAST of MS014539 vs. ExPASy TrEMBL
Match: A0A1S3C2H9 (RHOMBOID-like protein 9, chloroplastic isoform X4 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 7.4e-160
Identity = 311/475 (65.47%), Postives = 359/475 (75.58%), Query Frame = 0

Query: 4   VPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVL-KNPPSIL----NRKQWQEISSY 63
           VPT +  R  ND +NPTQ I  PNK    +C+C  V+ KN   IL        W EI + 
Sbjct: 3   VPTFFQIRNFNDLLNPTQTISKPNKRVC-ICKCDVVVSKNNLPILVTYSKHHNW-EIHNN 62

Query: 64  GLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRSSG 123
             S V I+   RKS  G +                          W+C +TNEKQLRS G
Sbjct: 63  DSSVVKISTRARKSIGGLKALLQHVRCSNGLNFEYSKVLEKLSQVWKC-TTNEKQLRSLG 122

Query: 124 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDK-DKDLHNHTLSAF 183
           SYFGRL+G G+K+  DSL+K++VLDTGQF AKKELQLLD YF+KVDK D DLH H LS+F
Sbjct: 123 SYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKADTDLHKHPLSSF 182

Query: 184 DEQNEGKLFFTSSPFED---QEENTPIRHERFNDTI------STTVQEDEISDLYLISSL 243
           D+Q EGKL FT+SPFED    ++ + I+H + +         +T +QE+E+ DLYLIS+L
Sbjct: 183 DDQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYLISAL 242

Query: 244 VSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVG 303
           VS+NIAVFLFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV 
Sbjct: 243 VSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVA 302

Query: 304 LSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWL 363
           LSCW LLTFGRQVCR+YGPFTFFLIYVLGGVSGN TSFLHTP PTVGGTGPVFAMIGAWL
Sbjct: 303 LSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAMIGAWL 362

Query: 364 SYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPI 423
           SYQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+YGFLTSP+
Sbjct: 363 SYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPL 422

Query: 424 VEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
            +V+D+SS    SSRRGQ++GIK+VRKYA+PCKSFAFF LFI+ F SL FF+QPP
Sbjct: 423 GKVNDASS----SSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPP 470

BLAST of MS014539 vs. ExPASy TrEMBL
Match: A0A0A0L0I7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G094530 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 2.2e-159
Identity = 314/475 (66.11%), Postives = 361/475 (76.00%), Query Frame = 0

Query: 1   MAVVPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVLK--NPPSIL--NRKQWQEIS 60
           MAVVPT +  R  N++I+       PNK    VC+   V+   N P+ +  ++    EI 
Sbjct: 1   MAVVPTFFQIRNFNETISK------PNKRVC-VCKWDVVVSKDNLPTFVSYSKHHTWEIH 60

Query: 61  SYGLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRS 120
               S V+I+   RKSRRG +                          WEC STNEKQLRS
Sbjct: 61  DNDSSVVTISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWEC-STNEKQLRS 120

Query: 121 SGSYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSA 180
            GSYFGRL+G G+++  DSL+K++VLDTGQF AKKELQLLD YF+KV  DK LH H LS+
Sbjct: 121 LGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKV--DKGLHKHPLSS 180

Query: 181 FDEQNEGKLFFTSSPFED--QEENTPIRHERFNDTI------STTVQEDEISDLYLISSL 240
           FDEQ EGKL FT+SP ED  +++   IRH + +  I       T +QE+E+ DLYLIS+L
Sbjct: 181 FDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISAL 240

Query: 241 VSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVG 300
           VS+NIAVFLFE+ASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFLHSGV HV 
Sbjct: 241 VSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVA 300

Query: 301 LSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWL 360
           LSCW LLTFGRQVCREYGPFTFFLIYVLGGVSGN TSFLHTP+PTVGGTGPVFAMIGAWL
Sbjct: 301 LSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWL 360

Query: 361 SYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPI 420
           SYQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+YGFLTSPI
Sbjct: 361 SYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPI 420

Query: 421 VEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFIQPP 441
           VEV+D+SSSS  SSRRGQ++GIKLVRKYA+PC+S AFF LFI+ F SL FFIQPP
Sbjct: 421 VEVNDASSSS--SSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 463

BLAST of MS014539 vs. ExPASy TrEMBL
Match: A0A1S4E1D5 (RHOMBOID-like protein 9, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.4e-158
Identity = 310/483 (64.18%), Postives = 359/483 (74.33%), Query Frame = 0

Query: 4   VPTIYYARYHNDSINPTQIIFGPNKLAYNVCECSPVL-KNPPSIL----NRKQWQEISSY 63
           VPT +  R  ND +NPTQ I  PNK    +C+C  V+ KN   IL        W EI + 
Sbjct: 3   VPTFFQIRNFNDLLNPTQTISKPNKRVC-ICKCDVVVSKNNLPILVTYSKHHNW-EIHNN 62

Query: 64  GLSAVSIAAGTRKSRRGFE-----------------------PHAWECSSTNEKQLRSSG 123
             S V I+   RKS  G +                          W+C +TNEKQLRS G
Sbjct: 63  DSSVVKISTRARKSIGGLKALLQHVRCSNGLNFEYSKVLEKLSQVWKC-TTNEKQLRSLG 122

Query: 124 SYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLHNHTLSAFD 183
           SYFGRL+G G+K+  DSL+K++VLDTGQF AKKELQLLD YF+KVDKD DLH H LS+FD
Sbjct: 123 SYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPLSSFD 182

Query: 184 EQNEGKLFFTSSPFED---QEENTPIRHERFNDTI------STTVQEDEISDLYLI---- 243
           +Q EGKL FT+SPFED    ++ + I+H + +         +T +QE+E+ DLYL+    
Sbjct: 183 DQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYLMLLLE 242

Query: 244 -----SSLVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFL 303
                S+LVS+NIAVFLFEIASPVKNSELQLFSLPS+YGAKINELILVGEWWRLVTPMFL
Sbjct: 243 IYICRSALVSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFL 302

Query: 304 HSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPV 363
           HSGV HV LSCW LLTFGRQVCR+YGPFTFFLIYVLGGVSGN TSFLHTP PTVGGTGPV
Sbjct: 303 HSGVLHVALSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPV 362

Query: 364 FAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMV 423
           FAMIGAWLSYQFQNK V+ KDV D MFLKA++AAVISSILSNIGPIDEWTHTGAAFSGM+
Sbjct: 363 FAMIGAWLSYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGML 422

Query: 424 YGFLTSPIVEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFALFILVFFSLPFFI 441
           YGFLTSP+ +V+D+SS    SSRRGQ++GIK+VRKYA+PCKSFAFF LFI+ F SL FF+
Sbjct: 423 YGFLTSPLGKVNDASS----SSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFV 478

BLAST of MS014539 vs. TAIR 10
Match: AT5G38510.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-76
Identity = 162/362 (44.75%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 88  EKQLRSSGSYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLH 147
           +++L+   SYFG+L+    K    + + I+     +    +EL  L  Y +K+ KD    
Sbjct: 77  KQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDSLSAYLDKLQKDAKSK 136

Query: 148 NHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRHERFNDTISTTVQEDEISDLYLISSLVS 207
               S  D          S   +   EN     ++F+D      Q ++  + Y +S L S
Sbjct: 137 GLVSSTLDVVKSEGGSVASKLRKTGIENNNSPFQQFDD----EDQAEDTLNFYAVSILAS 196

Query: 208 VNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGLS 267
           +N+ V LFE A+PV+N+ + L SLP +YGAKIN+LIL GEWWRLVTPMFLHSG+ HV LS
Sbjct: 197 INVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLILAGEWWRLVTPMFLHSGIPHVALS 256

Query: 268 CWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLSY 327
            WALLTFG +VCR+YG FTF LIY+LGGVSGNF SFLHT +PTVGGTGP FA+IGAWL  
Sbjct: 257 SWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSFLHTADPTVGGTGPAFALIGAWLVD 316

Query: 328 QFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIVE 387
           Q QNK +I  +  +++F KAII      ILS+ GPID+WT+ GA  +G+VYGF T P+++
Sbjct: 317 QNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPIDDWTNLGALIAGIVYGFFTCPVLQ 376

Query: 388 VDDSSSSSSPSSRRGQQEGIKLV----RKYADPCKSFAFFALFILVFFSLPFFIQPPPTT 446
           +    S         +QEGI  V    +  ADPCKSF  F +F+ V  +    I   P  
Sbjct: 377 LGSGGSE--------RQEGIVTVGPEKQNSADPCKSFLLFTIFVAVIVTSLLLIGDGPLD 424

BLAST of MS014539 vs. TAIR 10
Match: AT5G38510.2 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-76
Identity = 162/362 (44.75%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 88  EKQLRSSGSYFGRLKGGGNKRKFDSLEKIEVLDTGQFTAKKELQLLDDYFEKVDKDKDLH 147
           +++L+   SYFG+L+    K    + + I+     +    +EL  L  Y +K+ KD    
Sbjct: 77  KQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDSLSAYLDKLQKDAKSK 136

Query: 148 NHTLSAFDEQNEGKLFFTSSPFEDQEENTPIRHERFNDTISTTVQEDEISDLYLISSLVS 207
               S  D          S   +   EN     ++F+D      Q ++  + Y +S L S
Sbjct: 137 GLVSSTLDVVKSEGGSVASKLRKTGIENNNSPFQQFDD----EDQAEDTLNFYAVSILAS 196

Query: 208 VNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHVGLS 267
           +N+ V LFE A+PV+N+ + L SLP +YGAKIN+LIL GEWWRLVTPMFLHSG+ HV LS
Sbjct: 197 INVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLILAGEWWRLVTPMFLHSGIPHVALS 256

Query: 268 CWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAWLSY 327
            WALLTFG +VCR+YG FTF LIY+LGGVSGNF SFLHT +PTVGGTGP FA+IGAWL  
Sbjct: 257 SWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSFLHTADPTVGGTGPAFALIGAWLVD 316

Query: 328 QFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSPIVE 387
           Q QNK +I  +  +++F KAII      ILS+ GPID+WT+ GA  +G+VYGF T P+++
Sbjct: 317 QNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPIDDWTNLGALIAGIVYGFFTCPVLQ 376

Query: 388 VDDSSSSSSPSSRRGQQEGIKLV----RKYADPCKSFAFFALFILVFFSLPFFIQPPPTT 446
           +    S         +QEGI  V    +  ADPCKSF  F +F+ V  +    I   P  
Sbjct: 377 LGSGGSE--------RQEGIVTVGPEKQNSADPCKSFLLFTIFVAVIVTSLLLIGDGPLD 424

BLAST of MS014539 vs. TAIR 10
Match: AT1G25290.2 (RHOMBOID-like protein 10 )

HSP 1 Score: 83.6 bits (205), Expect = 4.5e-16
Identity = 51/180 (28.33%), Postives = 89/180 (49.44%), Query Frame = 0

Query: 205 LVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHV 264
           L+++N+ +++ +IAS  K            +GAKIN LI  G+ WRL T   LH+   H+
Sbjct: 133 LLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPMHL 192

Query: 265 GLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHTPEPTVGGTGPVFAMIGAW 324
            ++C++L + G       GP  F  +Y+   V+ +  S+     P+VG +G +F ++G+ 
Sbjct: 193 MINCYSLNSIGPTAESLGGPKRFLAVYLTSAVASSAMSYWFNKAPSVGASGAIFGLVGSV 252

Query: 325 LSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMVYGFLTSP 384
             +  ++K ++     D M +  IIA  ++  L +   ID W H G    G    +L  P
Sbjct: 253 AVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGGTAMTWLLGP 302

BLAST of MS014539 vs. TAIR 10
Match: AT1G25290.1 (RHOMBOID-like protein 10 )

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-14
Identity = 52/187 (27.81%), Postives = 90/187 (48.13%), Query Frame = 0

Query: 205 LVSVNIAVFLFEIASPVKNSELQLFSLPSVYGAKINELILVGEWWRLVTPMFLHSGVFHV 264
           L+++N+ +++ +IAS  K            +GAKIN LI  G+ WRL T   LH+   H+
Sbjct: 133 LLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPMHL 192

Query: 265 GLSCWALLTFGRQVCREYGPFTFFLIYVLGGVS-------GNFTSFLHTPEPTVGGTGPV 324
            ++C++L + G       GP  F  +Y+   V+       G+  S+     P+VG +G +
Sbjct: 193 MINCYSLNSIGPTAESLGGPKRFLAVYLTSAVAKPILRVLGSAMSYWFNKAPSVGASGAI 252

Query: 325 FAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDEWTHTGAAFSGMV 384
           F ++G+   +  ++K ++     D M +  IIA  ++  L +   ID W H G    G  
Sbjct: 253 FGLVGSVAVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGGTA 309

BLAST of MS014539 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 71.2 bits (173), Expect = 2.3e-12
Identity = 51/188 (27.13%), Postives = 89/188 (47.34%), Query Frame = 0

Query: 247 EWWRLVTPMFLHSGVFHVGLSCWALLTFGRQVCREYGPFTFFLIYVLGGVSGNFTSFLHT 306
           E WRL+T M+LH+G+ H+ ++ + ++ FG ++ +++G     LIY++ G  G+  S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 307 PEP-TVGGTGPVFAMIGAWLSYQFQNKGVIAKDVLDNMFLKAIIAAVISSILSNIGPIDE 366
            +  +VG +G +  ++GA LS    N  +    +   +    IIA  I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFLFIIA--INLAIGLLPWVDN 215

Query: 367 WTHTGAAFSGMVYGFLTSPIVEVDDSSSSSSPSSRRGQQEGIKLVRKYADPCKSFAFFAL 426
           + H G   +G   GF+            S     R   Q G +   KY +PC+   FF  
Sbjct: 216 FAHIGGLLTGFCLGFILLM------QPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVA 274

Query: 427 FILVFFSL 434
            +LV   L
Sbjct: 276 AVLVVAGL 274