MS014532 (gene) Bitter gourd (TR) v1

Overview
NameMS014532
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionExocyst subunit Exo70 family protein
Locationscaffold553: 682806 .. 684794 (-)
RNA-Seq ExpressionMS014532
SyntenyMS014532
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAGTGTGGGTGTTTGGTTTCAGAGCTTGAAGGAGAGAAACAGCTCATTGCTGCTGCTAATTTCATTATTAAGGCTTTAGGTTCTGACATAACCATTTCAGATGATGCAAAGAAGGTTTTGGCCGATCTTTGCACAGAGTTATCTTTAGTTGTTTCACGGAATCAGCGAGCCGATGTTGTCGAAATCGAAGTCGAGGGGACGGAGATTGGGGAAGTTGAAGAGATTGAGAGTAGGCTTAAAGTAGTGCAGGAGAAAATCATGGGTTGGGAAGCAGATCAGTCCATGATATGGGACTCTGCTGCTCCGGATGATGCTTCGGAGTATCTGAACGCTGCAGATGAAGCCCGGGGATTGGTTAGCAAGTTGGAGGGTTTGTGTTTGAGTAAAGATGAAGGAGATTTTGAACTGTCGAGGAAGGCGCACGATGTTCTTCAAACGGCTATGGCAAGGCTTGAGGAAGAGTTTAGGCACTTGCTTGCTAGAAGTAGCCTTGAATTTCAGCTTGAGAGTGTGTCTGTGCACTCCACGGAAGATGCTGTGGATGATGGATCGACACACTTATATCGAGATGAATCGTTTGAGGCTTCGGTTCGGAGTAGTAGTGTTGGCAGAAACTTGGAGAATTCTATCATCGATTTGGTCAACCCAGATGCGGTTTGTGAGCTCATGGGCATAGCAAATGTCATGTTCAAGTCTGGTTATGATCAGGAGTGCATCCAGGCTTACAATCATCTTCGTCGGGACGCTTTAAATGAGTGCCTCTTGACTCTTGAAATGGAGAAAATGAGCATTGAGGATGTGCTGAAAATGGATTGGGTTACCTTGAACTCGAAGATCAGGAAATGGAACCGAGCAATGAAGCGATTTGTGCGGGTCTATCTACCTAGCGAAAAATGCCTTAGTGATCAAATTTTTGGGGAGGAAGGATCGGTTAGTTTGACTTGTTTTGTTGAATCATCAAAGGCATCAATGCTGCAATTTCTGAACTTTGGCGAGGCCATGGCCATAGGCCCTCATACACCAGAAAAATTGAATCGCATTCTCGAAATGTATGAGGTCGTTGAAGAGCTTCTCTCGGATATCGATGCGTTATACTGTGATGAAGTTGGTTATCATGTTAGGATTGAGTATCATGATGTTCAAAAGAGTCTAGGACATTCTGTGAGAGCTACATTTCTCGAGTTCGGGAAAGCTATTGCGGCAAACACGTCACCGCACCCGTTTGCAGGCGGTGGAATCCATCATCTAACCAAATATGTGATGAATTACCTTATGATTCTTACCGATTATAGCGAATCCCTGAATTTTCTCCTCAAGGATGGAGAAGATATGTATCCCATTTCACCATCATGCTCTATGAATTCAAGTAGAGAAAGAGAAGAAGAAAGCGAAAGCAGAGGTTCGTGTTCAGGCGAGTTCTCCCCGATGGCTCGCCATTTTCGTTCGGTTGCTACAATTCTAGAGAATAACCTCGAGGAGAAGTCGAAGCAGTACAAAGATGCTGCTCTACAGCAATTCTTCTTGATGAACAACATACATTACATGGCTCAAAAGGTGAGGGGTTCGGAGCTTAGCCAGATATTCGGGGAGGATTGGATTCGTAAACACTACGGGAAGTTCCAGCAGCAGGCGATGAACTACGAGAGGGCTAGTTGGAATTCAATACTCTCATACCTTAGGGAGGATCCCGGTTCTAATTCGGAGTCGAAAAACGTGCTGAAAGAGAGGTTGCGCAGCTTCAATGTTGCTTTCGAGGAGATTTACAAAACCCAAACCTCGTGGATCATTCACGACTCTCGGCTTCGTGAAGATCTGCGGATCTCGACGTCGCTGAGGGTGATCCATGCATATAGAGCATTCTTTGGAAGGTGTAATAACCATGTAAGTGACAAGCTTATCAAGTACAGTCCAGATGATTTGGAGGGTTACCTTCTTGATCTCTTTGAGGGATCTCCAAAATCATTGCCCAACACCAATAGGAGG

mRNA sequence

ATGGGGGAGTGTGGGTGTTTGGTTTCAGAGCTTGAAGGAGAGAAACAGCTCATTGCTGCTGCTAATTTCATTATTAAGGCTTTAGGTTCTGACATAACCATTTCAGATGATGCAAAGAAGGTTTTGGCCGATCTTTGCACAGAGTTATCTTTAGTTGTTTCACGGAATCAGCGAGCCGATGTTGTCGAAATCGAAGTCGAGGGGACGGAGATTGGGGAAGTTGAAGAGATTGAGAGTAGGCTTAAAGTAGTGCAGGAGAAAATCATGGGTTGGGAAGCAGATCAGTCCATGATATGGGACTCTGCTGCTCCGGATGATGCTTCGGAGTATCTGAACGCTGCAGATGAAGCCCGGGGATTGGTTAGCAAGTTGGAGGGTTTGTGTTTGAGTAAAGATGAAGGAGATTTTGAACTGTCGAGGAAGGCGCACGATGTTCTTCAAACGGCTATGGCAAGGCTTGAGGAAGAGTTTAGGCACTTGCTTGCTAGAAGTAGCCTTGAATTTCAGCTTGAGAGTGTGTCTGTGCACTCCACGGAAGATGCTGTGGATGATGGATCGACACACTTATATCGAGATGAATCGTTTGAGGCTTCGGTTCGGAGTAGTAGTGTTGGCAGAAACTTGGAGAATTCTATCATCGATTTGGTCAACCCAGATGCGGTTTGTGAGCTCATGGGCATAGCAAATGTCATGTTCAAGTCTGGTTATGATCAGGAGTGCATCCAGGCTTACAATCATCTTCGTCGGGACGCTTTAAATGAGTGCCTCTTGACTCTTGAAATGGAGAAAATGAGCATTGAGGATGTGCTGAAAATGGATTGGGTTACCTTGAACTCGAAGATCAGGAAATGGAACCGAGCAATGAAGCGATTTGTGCGGGTCTATCTACCTAGCGAAAAATGCCTTAGTGATCAAATTTTTGGGGAGGAAGGATCGGTTAGTTTGACTTGTTTTGTTGAATCATCAAAGGCATCAATGCTGCAATTTCTGAACTTTGGCGAGGCCATGGCCATAGGCCCTCATACACCAGAAAAATTGAATCGCATTCTCGAAATGTATGAGGTCGTTGAAGAGCTTCTCTCGGATATCGATGCGTTATACTGTGATGAAGTTGGTTATCATGTTAGGATTGAGTATCATGATGTTCAAAAGAGTCTAGGACATTCTGTGAGAGCTACATTTCTCGAGTTCGGGAAAGCTATTGCGGCAAACACGTCACCGCACCCGTTTGCAGGCGGTGGAATCCATCATCTAACCAAATATGTGATGAATTACCTTATGATTCTTACCGATTATAGCGAATCCCTGAATTTTCTCCTCAAGGATGGAGAAGATATGTATCCCATTTCACCATCATGCTCTATGAATTCAAGTAGAGAAAGAGAAGAAGAAAGCGAAAGCAGAGGTTCGTGTTCAGGCGAGTTCTCCCCGATGGCTCGCCATTTTCGTTCGGTTGCTACAATTCTAGAGAATAACCTCGAGGAGAAGTCGAAGCAGTACAAAGATGCTGCTCTACAGCAATTCTTCTTGATGAACAACATACATTACATGGCTCAAAAGGTGAGGGGTTCGGAGCTTAGCCAGATATTCGGGGAGGATTGGATTCGTAAACACTACGGGAAGTTCCAGCAGCAGGCGATGAACTACGAGAGGGCTAGTTGGAATTCAATACTCTCATACCTTAGGGAGGATCCCGGTTCTAATTCGGAGTCGAAAAACGTGCTGAAAGAGAGGTTGCGCAGCTTCAATGTTGCTTTCGAGGAGATTTACAAAACCCAAACCTCGTGGATCATTCACGACTCTCGGCTTCGTGAAGATCTGCGGATCTCGACGTCGCTGAGGGTGATCCATGCATATAGAGCATTCTTTGGAAGGTGTAATAACCATGTAAGTGACAAGCTTATCAAGTACAGTCCAGATGATTTGGAGGGTTACCTTCTTGATCTCTTTGAGGGATCTCCAAAATCATTGCCCAACACCAATAGGAGG

Coding sequence (CDS)

ATGGGGGAGTGTGGGTGTTTGGTTTCAGAGCTTGAAGGAGAGAAACAGCTCATTGCTGCTGCTAATTTCATTATTAAGGCTTTAGGTTCTGACATAACCATTTCAGATGATGCAAAGAAGGTTTTGGCCGATCTTTGCACAGAGTTATCTTTAGTTGTTTCACGGAATCAGCGAGCCGATGTTGTCGAAATCGAAGTCGAGGGGACGGAGATTGGGGAAGTTGAAGAGATTGAGAGTAGGCTTAAAGTAGTGCAGGAGAAAATCATGGGTTGGGAAGCAGATCAGTCCATGATATGGGACTCTGCTGCTCCGGATGATGCTTCGGAGTATCTGAACGCTGCAGATGAAGCCCGGGGATTGGTTAGCAAGTTGGAGGGTTTGTGTTTGAGTAAAGATGAAGGAGATTTTGAACTGTCGAGGAAGGCGCACGATGTTCTTCAAACGGCTATGGCAAGGCTTGAGGAAGAGTTTAGGCACTTGCTTGCTAGAAGTAGCCTTGAATTTCAGCTTGAGAGTGTGTCTGTGCACTCCACGGAAGATGCTGTGGATGATGGATCGACACACTTATATCGAGATGAATCGTTTGAGGCTTCGGTTCGGAGTAGTAGTGTTGGCAGAAACTTGGAGAATTCTATCATCGATTTGGTCAACCCAGATGCGGTTTGTGAGCTCATGGGCATAGCAAATGTCATGTTCAAGTCTGGTTATGATCAGGAGTGCATCCAGGCTTACAATCATCTTCGTCGGGACGCTTTAAATGAGTGCCTCTTGACTCTTGAAATGGAGAAAATGAGCATTGAGGATGTGCTGAAAATGGATTGGGTTACCTTGAACTCGAAGATCAGGAAATGGAACCGAGCAATGAAGCGATTTGTGCGGGTCTATCTACCTAGCGAAAAATGCCTTAGTGATCAAATTTTTGGGGAGGAAGGATCGGTTAGTTTGACTTGTTTTGTTGAATCATCAAAGGCATCAATGCTGCAATTTCTGAACTTTGGCGAGGCCATGGCCATAGGCCCTCATACACCAGAAAAATTGAATCGCATTCTCGAAATGTATGAGGTCGTTGAAGAGCTTCTCTCGGATATCGATGCGTTATACTGTGATGAAGTTGGTTATCATGTTAGGATTGAGTATCATGATGTTCAAAAGAGTCTAGGACATTCTGTGAGAGCTACATTTCTCGAGTTCGGGAAAGCTATTGCGGCAAACACGTCACCGCACCCGTTTGCAGGCGGTGGAATCCATCATCTAACCAAATATGTGATGAATTACCTTATGATTCTTACCGATTATAGCGAATCCCTGAATTTTCTCCTCAAGGATGGAGAAGATATGTATCCCATTTCACCATCATGCTCTATGAATTCAAGTAGAGAAAGAGAAGAAGAAAGCGAAAGCAGAGGTTCGTGTTCAGGCGAGTTCTCCCCGATGGCTCGCCATTTTCGTTCGGTTGCTACAATTCTAGAGAATAACCTCGAGGAGAAGTCGAAGCAGTACAAAGATGCTGCTCTACAGCAATTCTTCTTGATGAACAACATACATTACATGGCTCAAAAGGTGAGGGGTTCGGAGCTTAGCCAGATATTCGGGGAGGATTGGATTCGTAAACACTACGGGAAGTTCCAGCAGCAGGCGATGAACTACGAGAGGGCTAGTTGGAATTCAATACTCTCATACCTTAGGGAGGATCCCGGTTCTAATTCGGAGTCGAAAAACGTGCTGAAAGAGAGGTTGCGCAGCTTCAATGTTGCTTTCGAGGAGATTTACAAAACCCAAACCTCGTGGATCATTCACGACTCTCGGCTTCGTGAAGATCTGCGGATCTCGACGTCGCTGAGGGTGATCCATGCATATAGAGCATTCTTTGGAAGGTGTAATAACCATGTAAGTGACAAGCTTATCAAGTACAGTCCAGATGATTTGGAGGGTTACCTTCTTGATCTCTTTGAGGGATCTCCAAAATCATTGCCCAACACCAATAGGAGG

Protein sequence

MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTEDAVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNTNRR
Homology
BLAST of MS014532 vs. NCBI nr
Match: XP_022155076.1 (exocyst complex component EXO70E2 [Momordica charantia])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 659/663 (99.40%), Postives = 659/663 (99.40%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGECGCLVSEL GEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD
Sbjct: 1   MGECGCLVSELGGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVEIEVEGTEIGEVEEIESRLKVVQEKIM WEADQSMIWDSAAPDDASEYLNAADEAR L
Sbjct: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMVWEADQSMIWDSAAPDDASEYLNAADEAREL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED
Sbjct: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
           AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC
Sbjct: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK
Sbjct: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
           CL DQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL
Sbjct: 301 CLCDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
           SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK
Sbjct: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR
Sbjct: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG
Sbjct: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540

Query: 541 KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD 600
           KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD
Sbjct: 541 KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD 600

Query: 601 SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT 660
           SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT
Sbjct: 601 SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT 660

Query: 661 NRR 664
           NRR
Sbjct: 661 NRR 663

BLAST of MS014532 vs. NCBI nr
Match: XP_038897591.1 (exocyst complex component EXO70E2 [Benincasa hispida])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.2e-306
Identity = 545/668 (81.59%), Postives = 593/668 (88.77%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRN-QRA 60
           MGEC CLVSE  GE+QL+AAAN+IIKAL S+I++SDD KKVLADLC++LSLV ++N Q  
Sbjct: 1   MGECECLVSESGGEEQLVAAANYIIKALSSNISLSDDGKKVLADLCSKLSLVTTQNHQET 60

Query: 61  DVVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARG 120
           DVVE+E++ TE+G+VEEIESR  VVQEKIMGWEADQSMIWDS  P++A +YLNAADEAR 
Sbjct: 61  DVVEVEIKETEVGDVEEIESRFIVVQEKIMGWEADQSMIWDSTTPNEACDYLNAADEARE 120

Query: 121 LVSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTE 180
           LV +LE LCLSKDE  +ELSRKAHDVLQ AMARLEEEF+HLLA+SSLE + ESVS H  E
Sbjct: 121 LVGQLESLCLSKDEQGYELSRKAHDVLQIAMARLEEEFKHLLAKSSLEDEPESVSFHVAE 180

Query: 181 DAVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQE 240
           DAV+DGST LYRDESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQE
Sbjct: 181 DAVEDGSTSLYRDESFESSVRSSSVGRILENSIIDLVNPDAVIELRGIANVMFKAGYDQE 240

Query: 241 CIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSE 300
           CIQAYNHLRRDALNECLLTLEMEK+SIEDVLKMDW +LNSKIRKWNRAMKRFVR+YL SE
Sbjct: 241 CIQAYNHLRRDALNECLLTLEMEKLSIEDVLKMDWGSLNSKIRKWNRAMKRFVRIYLASE 300

Query: 301 KCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEEL 360
           + L DQIFGEEG VSL CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEV+EE 
Sbjct: 301 RSLCDQIFGEEGLVSLNCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVIEEH 360

Query: 361 LSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLT 420
           L DID LYCD+VGY VRIEYHDV  SLG SVRATFLEF KAIAANTSP+PFAGGGIHHLT
Sbjct: 361 LLDIDTLYCDDVGYLVRIEYHDVLNSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLT 420

Query: 421 KYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMA 480
           KYVMNYL+ILTDYS+SLN LLKD ED+ P SPS S+N +RE + E        GEFSPMA
Sbjct: 421 KYVMNYLVILTDYSDSLNLLLKDDEDVCPNSPSSSLNPTREEDRE--------GEFSPMA 480

Query: 481 RHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHY 540
           RHFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL QIFGEDW+RKHY
Sbjct: 481 RHFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIQIFGEDWVRKHY 540

Query: 541 GKFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTS 600
            KFQQQA NYERASWNSILSYLRED    PGSN+ SKNVLK+RLRSFN+AFEEIYKTQT+
Sbjct: 541 KKFQQQATNYERASWNSILSYLREDGIQNPGSNTVSKNVLKDRLRSFNLAFEEIYKTQTA 600

Query: 601 WIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPK 660
           WIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDK IKY PDDLEGYLLDLFEGSPK
Sbjct: 601 WIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKFIKYYPDDLEGYLLDLFEGSPK 660

Query: 661 SLPNTNRR 664
           SL N +RR
Sbjct: 661 SLANNSRR 660

BLAST of MS014532 vs. NCBI nr
Match: XP_008462929.1 (PREDICTED: exocyst complex component EXO70B1 [Cucumis melo] >KAA0061338.1 exocyst complex component EXO70B1 [Cucumis melo var. makuwa] >TYK14692.1 exocyst complex component EXO70B1 [Cucumis melo var. makuwa])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.3e-303
Identity = 540/667 (80.96%), Postives = 585/667 (87.71%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGEC CLVSE  GE++L+AAAN+IIKAL S+I ISDD KKVLADLC++LSLV ++N   D
Sbjct: 1   MGECECLVSESVGEERLVAAANYIIKALSSNIRISDDGKKVLADLCSKLSLVTTQNYETD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVE+E+    +G+VEEIES   VVQEKIM WEADQSMIWDS   ++A EYLNAADEAR L
Sbjct: 61  VVEVEINERGVGDVEEIESSFNVVQEKIMAWEADQSMIWDSTTLNEACEYLNAADEAREL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KL+ LCLSKDE  +EL RKAHDVLQTAMARLEEEFRHLLA+SSLE++ ES+S H  ED
Sbjct: 121 VGKLDSLCLSKDEYGYELLRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESMSFHVVED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
            V+DGS+ LY DESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQEC
Sbjct: 181 TVEDGSSSLYHDESFESSVRSSSVGRVLENSIIDLVNPDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQ YN LRRDALNECLLTLEMEK+SIEDVLKMDWVTLNSKIRKWNRAMKRFVR+YL SEK
Sbjct: 241 IQVYNLLRRDALNECLLTLEMEKLSIEDVLKMDWVTLNSKIRKWNRAMKRFVRIYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQIFGEEG VSL+CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEVVEE L
Sbjct: 301 SLCDQIFGEEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVVEEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
            DID LYCD++GY VRIEYHDV KSLG SVRATFLEF KAIAANTSP+PFAGGGIHHLTK
Sbjct: 361 FDIDTLYCDDIGYLVRIEYHDVLKSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDY +SLN LLKD ED+ P SPS S+N SRE + E        GEFSPMAR
Sbjct: 421 YVMNYLMILTDYRDSLNLLLKDDEDVCPNSPSSSLNPSREEDRE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL QIFGEDW+RKHY 
Sbjct: 481 HFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIQIFGEDWVRKHYK 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
           KFQQQA NYERASWNSIL YLRED    PGS S SKNVLK+RLRSFN+AFEEIYKTQT+W
Sbjct: 541 KFQQQATNYERASWNSILQYLREDGIQNPGSTSVSKNVLKDRLRSFNLAFEEIYKTQTAW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           IIHDSRLREDLRISTSLRVIHAYRAF+GRCNNHVSDKLIKY+PDDLEGYLLDLFEGSPKS
Sbjct: 601 IIHDSRLREDLRISTSLRVIHAYRAFYGRCNNHVSDKLIKYTPDDLEGYLLDLFEGSPKS 659

Query: 661 LPNTNRR 664
           L NT+RR
Sbjct: 661 LANTSRR 659

BLAST of MS014532 vs. NCBI nr
Match: XP_011653366.1 (exocyst complex component EXO70E2 [Cucumis sativus] >KGN53679.1 hypothetical protein Csa_015217 [Cucumis sativus])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.7e-303
Identity = 539/667 (80.81%), Postives = 586/667 (87.86%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGEC CLVSE  GE++L+AAAN+IIKAL S+I ISDD KKVLADLC++LSLV ++N   D
Sbjct: 1   MGECECLVSESGGEERLVAAANYIIKALSSNIRISDDGKKVLADLCSKLSLVSTQNYETD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVE+E+E   +G+VEE+ESR  VVQEKIM WEADQSMIWDS   ++A EYLNAADEA  L
Sbjct: 61  VVEVEIEERGVGDVEEVESRFNVVQEKIMAWEADQSMIWDSTTLNEACEYLNAADEAWDL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KL+ LCLSKDE  +EL RKAHDVLQTAMARLEEEFRHLLA+SSLE++ ES+S H  ED
Sbjct: 121 VGKLDSLCLSKDEYSYELLRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESMSFHVVED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
            V+DGST LYRDESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQEC
Sbjct: 181 TVEDGSTSLYRDESFESSVRSSSVGRVLENSIIDLVNPDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQ YN LRRDALNECLLTLEMEK+SIEDVLKMDWVTLNSKIRKWNRAMKRFVR+YL SEK
Sbjct: 241 IQVYNLLRRDALNECLLTLEMEKLSIEDVLKMDWVTLNSKIRKWNRAMKRFVRIYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQIFGEEG VSL+CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEVVEE L
Sbjct: 301 SLCDQIFGEEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVVEEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
            DID LYCD++GY VRIEYHDV KSLG SVRATFLEF KAIAANTSP+PFAGGGIHHLTK
Sbjct: 361 FDIDTLYCDDIGYLVRIEYHDVLKSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDY +SLN LLKD ED+ P SPS S+N +RE + E        GEFSPMAR
Sbjct: 421 YVMNYLMILTDYRDSLNLLLKDDEDVCPNSPSSSLNPTREEDRE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL +IFGEDW+RKHY 
Sbjct: 481 HFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIRIFGEDWVRKHYK 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
           KFQQQA NYERASWNSIL YLRED     GS S SKNVLK+RLRSFN+AFEEIYKTQT+W
Sbjct: 541 KFQQQATNYERASWNSILQYLREDGIQNTGSTSVSKNVLKDRLRSFNLAFEEIYKTQTAW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           IIHDSRLREDLRISTSLRVIHAYRAF+GRCNNHVSDKLIKY+PDDLEGYLLDLFEGSPKS
Sbjct: 601 IIHDSRLREDLRISTSLRVIHAYRAFYGRCNNHVSDKLIKYTPDDLEGYLLDLFEGSPKS 659

Query: 661 LPNTNRR 664
           L NT+RR
Sbjct: 661 LANTSRR 659

BLAST of MS014532 vs. NCBI nr
Match: KAG7036789.1 (Exocyst complex component EXO70B1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.7 bits (2612), Expect = 5.6e-291
Identity = 524/667 (78.56%), Postives = 577/667 (86.51%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MG+C  + S+L GE+QLIAAAN IIKAL S+I++SDD K V+ DLC++LSL+ ++N +  
Sbjct: 1   MGDCEFVGSDLVGEEQLIAAANCIIKALSSNISLSDDTKSVMVDLCSKLSLITTQNHQIG 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           V     +  ++G V+EIESRL VVQ+KIMGWEADQSMIWDSA P+DA +YLNAAD+A  L
Sbjct: 61  V----TKDRDVGLVDEIESRLNVVQKKIMGWEADQSMIWDSAFPNDACDYLNAADDAWEL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KLE LC  +DE  +ELSRKAHDVLQTAMARLEEEFRHLLA+SSLE++ ESVS H TED
Sbjct: 121 VGKLESLCF-EDEHGYELSRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESVSSHVTED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
           +V++GST LYRDESFE+SVRS SVGR LENSIIDLVN DAV EL GIANVMFK+GYDQEC
Sbjct: 181 SVEEGSTSLYRDESFESSVRSCSVGRTLENSIIDLVNSDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQAYNHLRR+ALNECL  LEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYL SEK
Sbjct: 241 IQAYNHLRREALNECLSMLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQ+FG+EG VSL+CFVESSKASMLQ LNFGEAMAIGPH PEKLNRILEMYEVV E L
Sbjct: 301 FLCDQVFGDEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHPPEKLNRILEMYEVVGEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
           SDID LYCD+VGY VRIEYHDV  SLG SV ATFLEFGKAIAANTSP+ FAGGGIHHLTK
Sbjct: 361 SDIDTLYCDDVGYLVRIEYHDVLNSLGKSVTATFLEFGKAIAANTSPNAFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYL+ILTDY +SLN LL DGED+YP SPS S N SRE E+E        GEFSPMAR
Sbjct: 421 YVMNYLLILTDYGDSLNVLLGDGEDVYPNSPSSSENPSREEEKE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVATILE+NLEEKSKQYKD ALQ FFLMNNIHYMAQKVRGSELSQIFGEDW+RK Y 
Sbjct: 481 HFRSVATILESNLEEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELSQIFGEDWVRKRYM 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
            FQQ  +NY+RASWNSI+ YL ED    PGSNS S+NVLKERLRSFNVAFEEIYKTQTSW
Sbjct: 541 NFQQHVLNYKRASWNSIVLYLMEDGVQHPGSNSVSRNVLKERLRSFNVAFEEIYKTQTSW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           I+HDSRLR+DLRISTSL+VI AYRAFFGRCNNHVSDKLIKY+PDDLEG+LLDLFEGSPKS
Sbjct: 601 IVHDSRLRDDLRISTSLQVIPAYRAFFGRCNNHVSDKLIKYTPDDLEGFLLDLFEGSPKS 654

Query: 661 LPNTNRR 664
           L NTNRR
Sbjct: 661 LANTNRR 654

BLAST of MS014532 vs. ExPASy Swiss-Prot
Match: Q9FNR3 (Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 4.4e-121
Identity = 263/652 (40.34%), Postives = 386/652 (59.20%), Query Frame = 0

Query: 20  AANFIIKAL-GSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIGEVEEIE 79
           A + ++KAL  SD  +  + +K+L+DL   LS          + + +VE        EI+
Sbjct: 20  ACHHVVKALRASDNNLDANLRKLLSDLEMHLSTF-------GIADTKVEDAGF---SEIK 79

Query: 80  SRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKDEGDFEL 139
            R K   ++I  WE +QS +++ A   +A ++  A  + + ++   + L +  ++ + ++
Sbjct: 80  KRFKEAVKRIRSWETNQSTMFE-AGLSEADQFFQALYDVQTVLVGFKALPMKTNQMEKDV 139

Query: 140 SRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHS-TEDAVDDGSTHLYRDESF-E 199
             +A   L  AM RLE+E   +L +     Q + ++V S  +D V D S     DE   E
Sbjct: 140 YNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVSLDDEVIVE 199

Query: 200 ASVRSSSVG-RNLENSIIDLVNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRDALNEC 259
           AS         +  NS  DLV+P  +  +  IAN MF   YDQ   +A+  ++R+AL E 
Sbjct: 200 ASSHEDDEQISDFYNS--DLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQREALEEY 259

Query: 260 LLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSL 319
           ++TLEME+ S  DVL+MDW  LN  +RKW + +K   +VYL SEK L DQI G+  S+S 
Sbjct: 260 MVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGDFESIST 319

Query: 320 TCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHV 379
            CF+E SK ++L  LNFGEA+ +    PE L R L MYEV  E+L D+D L+ DE G  +
Sbjct: 320 ACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPDETGSSL 379

Query: 380 RIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSES 439
           RI +H++ K L      TFL+F  AIA++ S  PF GGGIHHLT+YVMNYL +L +Y++S
Sbjct: 380 RIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLKLLPEYTDS 439

Query: 440 LNFLLKD--GEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHFRSVATILENNL 499
           LN LL++   +D  P               E          FSPMARH RS+ T LE++L
Sbjct: 440 LNSLLQNIHVDDSIP---------------EKTGEDVLPSTFSPMARHLRSIVTTLESSL 499

Query: 500 EEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKFQQQAMNYERAS 559
           E K++ Y D AL+  FLMNN  YM QKV+GSEL ++FG++WIRKH   +Q    NYER++
Sbjct: 500 ERKAQLYADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERST 559

Query: 560 WNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSL 619
           W+SIL+ LR+    N++S   L+ER R F++AF+++YK QT W + DS LR+DL ISTS+
Sbjct: 560 WSSILALLRD----NNDSVRTLRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSV 619

Query: 620 RVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFE--GSPKSLPNTNRR 664
           +V+ +YR F GR    + +K I+Y+ +D+E  LLDLFE   SP+SL ++ +R
Sbjct: 620 KVVQSYRGFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of MS014532 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.2e-83
Identity = 208/659 (31.56%), Postives = 345/659 (52.35%), Query Frame = 0

Query: 13  GEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIG 72
           GE++L+A A  I K LG + +++DD  ++ ++          R  R  + E +  G +  
Sbjct: 5   GEEKLLAVARHIAKTLGHNESMADDILQIFSNF-------DGRFSREKLAEGQA-GEDGS 64

Query: 73  EVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKD 132
            V  +E  L  +  +I  + A    IW  A P D++ +L+  DE   ++ +   +   K 
Sbjct: 65  GVATLERALNSIDGQISRFVAADQPIW--ADPADSAAFLDTIDELVAIIREWSPMASEKP 124

Query: 133 EGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTEDAVDDGSTHLYRD 192
            G      +A D++Q AM R+EEEFR L+ R +     ES  ++   DA      H +  
Sbjct: 125 IG--ICLTRADDMMQQAMFRIEEEFRSLMERGA-----ESFGLNPQGDA--GAMNHRFDS 184

Query: 193 ESFEASVRSSSVGRNLE----------NSIIDLVNPDAVCELMGIANVMFKSGYDQECIQ 252
           E  E   R  + G +++          + IID +    + +L  +A  M  +G+ + C  
Sbjct: 185 EEEEDDDRDFNNGDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSH 244

Query: 253 AYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCL 312
            Y+  RR+ L E +  L ++K+SIE+V KM W  L  +I +W +A    +R+  PSE+ L
Sbjct: 245 VYSSCRREFLEESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRL 304

Query: 313 SDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSD 372
            D++F    S +   F+E  + S +Q LNF +A+AIG  +PE+L ++L+++E + +L+ +
Sbjct: 305 CDRVFFGFSSAADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPE 364

Query: 373 IDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYV 432
            ++++ D+    +R E   + K LG ++R  F+E    I  + +     GGG+H +T+YV
Sbjct: 365 FESVFSDQFCSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYV 424

Query: 433 MNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHF 492
           MNYL                         +C    + E+  E ES G  S + + +    
Sbjct: 425 MNYL-----------------------RAACRSRQTLEQVFE-ESNGVPSKDSTLLTVQM 484

Query: 493 RSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKF 552
             +  +LE+NLE KSK YKD AL   FLMNN  Y+ QKV+  +L  + G+DWIRKH  K 
Sbjct: 485 SWIMELLESNLEVKSKVYKDPALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKV 544

Query: 553 QQQAMNYERASWNSILSYLRED---PGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIH 612
           +Q  MNY+R+SWN +L  L+ D    G N   K  +KE+L+ FN+ F+EI K  ++W++ 
Sbjct: 545 KQYHMNYQRSSWNKMLGLLKVDNTAAGMNGLGK-TMKEKLKQFNIQFDEICKVHSTWVVF 604

Query: 613 DSRLREDLRISTSLRVIHAYRAFFGRCNN-----HVSDKLIKYSPDDLEGYLLDLFEGS 654
           D +L+E+L+IS +  ++ AY +F GR  N       +DK IKY  +D+E  + +LF+G+
Sbjct: 605 DEQLKEELKISLARLLVPAYGSFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKGT 619

BLAST of MS014532 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.1e-69
Identity = 179/578 (30.97%), Postives = 284/578 (49.13%), Query Frame = 0

Query: 105 DDASEYLNAADEARGLVSK-LEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLAR 164
           +D   YL+A  + R ++   +        +G   +   A+ +L  A ++LEEEF+ LLA 
Sbjct: 96  EDLESYLDAIAQLRKIIRYFMSNKSFKSSDG---VLNHANSLLAKAQSKLEEEFKQLLA- 155

Query: 165 SSLEFQLESVSVHSTEDAVDDGSTHLYRDESFEASVRSSSVGRNLENS-----IIDLVNP 224
                   S S     D + DG  +  R  S          G + +++      + ++ P
Sbjct: 156 --------SYSKAVEPDRLFDGLPNSLRPSSDGDGGGKPHGGHHNDDAETAAYTLPILIP 215

Query: 225 DAVCELM-GIANVMFKSGYDQECIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTL 284
             V  L+  +A  M ++G+ Q+ +Q Y   R   L E L  L +EK+S EDV +M W  L
Sbjct: 216 SRVLPLLHDLAQQMVQAGHQQQLLQIYRDTRSFVLEESLKKLGVEKLSKEDVQRMQWEVL 275

Query: 285 NSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMA 344
            +KI  W   M+  V++    E+ + DQIF    S+S  CF E + +S+   L+FG+A+A
Sbjct: 276 EAKIGNWIHFMRIAVKLLFAGERQVCDQIFRGFDSLSDQCFAEVTVSSVSMLLSFGDAIA 335

Query: 345 IGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEF 404
               +PEKL  +L+MYE++ EL ++I+ ++  +    +R     + K L  + + TF +F
Sbjct: 336 RSKRSPEKLFVLLDMYEIMRELHTEIETIFKGKACLEIRDSATGLTKRLAQTAQETFGDF 395

Query: 405 GKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNS 464
            +A+  + +      G +H LT YV+NY+  L DY  +L  L  +               
Sbjct: 396 EEAVEKDATKTAVLDGTVHPLTSYVINYVKFLFDYQTTLKQLFLE--------------- 455

Query: 465 SREREEESESRGSCSGEFSPMARHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYM 524
                      G+     S +A     +   L+NNL+ KSKQYKD AL   FLMNNIHYM
Sbjct: 456 ----------FGNGDDSNSQLASVTMRIMQALQNNLDGKSKQYKDPALTHLFLMNNIHYM 515

Query: 525 AQKVRGSELSQIFGEDWIRKHYGKFQQQAMNYERASWNSILSYLR------------EDP 584
            + VR SE   + G+DW+++H    QQ A  Y+R +W  IL                E  
Sbjct: 516 VRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAWTKILQSSSAQGLTSSGGGSLEGG 575

Query: 585 GSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSLRVIHAYRAFFGR 644
            S+  S+ +LKER + FN+ F+E+++ Q+ W + D+ LRE LR++ +  ++ AYR+F  R
Sbjct: 576 NSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTELRESLRLAVAEVLLPAYRSFLKR 635

Query: 645 CNNHVS-----DKLIKYSPDDLEGYLLDLFEGSPKSLP 659
               V       K IKY+ +DLE  L +LFEG   + P
Sbjct: 636 FGPLVESGKNPQKYIKYTAEDLERLLGELFEGKSMNEP 636

BLAST of MS014532 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 4.3e-68
Identity = 186/651 (28.57%), Postives = 310/651 (47.62%), Query Frame = 0

Query: 13  GEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIG 72
           G++ L AAA  I +ALG D + + D  ++L+          S N+  D       G   G
Sbjct: 5   GDENLYAAARDIARALGKDPSAAGDILQILS------GYGASGNRGGDPRPTPSRG---G 64

Query: 73  EVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKD 132
                +  L  ++ +I  +  +   IW    P D+  +L++ DE   +   L    ++ D
Sbjct: 65  SNVNFDRALTSLERQISSYIVEDRPIWSD--PVDSRTFLDSVDELLAIAGDLRS--MAGD 124

Query: 133 EGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTEDAVDDGSTHLYRD 192
           +       +A +++Q  M RL+EEF  ++ R+   F  +       ++   DG       
Sbjct: 125 KSVAVCQSRADELIQQVMFRLQEEFGFVMDRAPDSFDSDDEFPGEEDNDTSDGVI----- 184

Query: 193 ESFEASVRSSSVGRNLEN--SIIDLVNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRD 252
                      V R + +   +I+ +    + +L  IA  M   G+ +EC + Y+  RR+
Sbjct: 185 -----------VARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSRRRE 244

Query: 253 ALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGE- 312
            L E L  L +  +S+E+V +  W  L  +I +W +A+     V+ PSE+ L D++F + 
Sbjct: 245 FLEESLSRLHLRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVFSDL 304

Query: 313 -EGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDIDALYC 372
              SV+   F+E  + +  Q LNF +A+A+G   PE+L +++++YE +++L+  ++ L+ 
Sbjct: 305 PVSSVTDLSFMEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMETLFS 364

Query: 373 DEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMI 432
           D     +R E   + K LG ++R  F+E    I  +     F GGGIH +T+YVMNYL  
Sbjct: 365 DRYCSPLRHEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNYLRA 424

Query: 433 LTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHFRSVATI 492
                +SL  +L                     ++     GS   +  P++     V  +
Sbjct: 425 ACKSRQSLEQIL---------------------DQTGNETGS---DTRPLSVQIIWVLEL 484

Query: 493 LENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKFQQQAMN 552
           LE+NLE K + Y+D +L   F+MNN  Y+  K + +EL  + GEDWI KH  K +Q   N
Sbjct: 485 LESNLEGKKRTYRDPSLCFLFMMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSN 544

Query: 553 YERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLR 612
           Y R+SWN ++  LR D G   +    L E LR F   F+E+ K Q+ W++ D +LRE+LR
Sbjct: 545 YRRSSWNQVVGLLRTD-GPYPK----LVENLRLFKSQFDEVCKVQSQWVVSDGQLREELR 597

Query: 613 ISTSLRVIHAYRAFFGR------CNNHVSDKLIKYSPDDLEGYLLDLFEGS 654
            S +  V  AY  F  R       N    +  I Y+ +D+E  +  LF+ S
Sbjct: 605 SSVAGIVSPAYSNFIRRLKESPEINGRRGEPFIPYTVEDVEFIIKRLFKES 597

BLAST of MS014532 vs. ExPASy Swiss-Prot
Match: Q8VY27 (Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.8e-57
Identity = 150/554 (27.08%), Postives = 273/554 (49.28%), Query Frame = 0

Query: 106 DASEYLNAADEARGLVSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSS 165
           +A E++    + R  +  L    +S+D    +L+  A  ++Q AMARLE+EF  +L+ + 
Sbjct: 76  EAKEFIRCVRDLRRAMHFL----VSQDSQSPKLA-LAQTLMQIAMARLEKEFFQILSSNR 135

Query: 166 LEFQLESVSVHSTEDAVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELM 225
            +   ESVS  S+  +  +    +  D+  E+       G ++    ++  +   + +L 
Sbjct: 136 DKLDPESVSGQSSISSNSEFEDVMGSDDDDESDNEMRKAGESITQ--VEKASAMVMSDLK 195

Query: 226 GIANVMFKSGYDQECIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWN 285
            IA  M   GY +EC++ Y  +R+  ++E L  L +E        + DWVTL   I+ W 
Sbjct: 196 AIAESMISCGYGKECVKIYKRVRKSIVDEGLSLLGIEIYKGSRFHRTDWVTLEHMIKNWI 255

Query: 286 RAMKRFVRVYLPSEKCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEK 345
           +A K  +      EK L D +F    S   +CF E +  +      F E +A    + E+
Sbjct: 256 KAAKIGIATLFRGEKLLCDHVFSASNSTRESCFYEIANEAATNLFKFPEFVAKEKKSHER 315

Query: 346 LNRILEMYEVVEELLSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANT 405
           +  ++++   + +L  DI+ ++  +    V+ +     + L  S+ +   +F   I  +T
Sbjct: 316 IFPLMDLQAAISDLWQDIEMIFHFDAVAGVKSQALTSLQKLKVSIHSALTDFESIIQKDT 375

Query: 406 SPHPFAGGGIHHLTKYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEES 465
           +     GGGIH LT+  MN++  L+ YS  L+ +L D    +P+  +  +  S  R   S
Sbjct: 376 TKALTPGGGIHKLTRSTMNFISSLSKYSGVLSEILAD----HPLPRNTRLLESYVRAPIS 435

Query: 466 ESRGSCSGEFSPMARHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSE 525
           E           ++ HF  +  +L   L+ K++ YKD +L   FL NN+  + + V  + 
Sbjct: 436 ED----EQHNHALSVHFAWLILVLLCKLDTKAEHYKDVSLSYLFLANNLQIIIETVGSTP 495

Query: 526 LSQIFGEDWIRKHYGKFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVA 585
           L  + G+DW+ KH  K    A NYE A+W+++   L E+P   S  +   K   R F+ A
Sbjct: 496 LRNLLGDDWLNKHEDKLCAYAGNYEIAAWSNVFMSLPEEPTDLSPEE--AKIYFRRFHTA 555

Query: 586 FEEIYKTQTSWIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDK-----LIKYSPD 645
           FEE Y  Q+S ++ +++LR++L++S + +++  YR F+ +    +  +     L+++ PD
Sbjct: 556 FEEAYMKQSSRVVPNAKLRDELKVSIAKKLVPEYREFYRKYLPMLGQERNIEILVRFKPD 612

Query: 646 DLEGYLLDLFEGSP 655
           +LE Y+ DLF G+P
Sbjct: 616 NLENYISDLFHGTP 612

BLAST of MS014532 vs. ExPASy TrEMBL
Match: A0A6J1DQM1 (Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111022209 PE=3 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 659/663 (99.40%), Postives = 659/663 (99.40%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGECGCLVSEL GEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD
Sbjct: 1   MGECGCLVSELGGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVEIEVEGTEIGEVEEIESRLKVVQEKIM WEADQSMIWDSAAPDDASEYLNAADEAR L
Sbjct: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMVWEADQSMIWDSAAPDDASEYLNAADEAREL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED
Sbjct: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
           AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC
Sbjct: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK
Sbjct: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
           CL DQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL
Sbjct: 301 CLCDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
           SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK
Sbjct: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR
Sbjct: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG
Sbjct: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540

Query: 541 KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD 600
           KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD
Sbjct: 541 KFQQQAMNYERASWNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHD 600

Query: 601 SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT 660
           SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT
Sbjct: 601 SRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKSLPNT 660

Query: 661 NRR 664
           NRR
Sbjct: 661 NRR 663

BLAST of MS014532 vs. ExPASy TrEMBL
Match: A0A5A7V1A0 (Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold692G00140 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 6.2e-304
Identity = 540/667 (80.96%), Postives = 585/667 (87.71%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGEC CLVSE  GE++L+AAAN+IIKAL S+I ISDD KKVLADLC++LSLV ++N   D
Sbjct: 1   MGECECLVSESVGEERLVAAANYIIKALSSNIRISDDGKKVLADLCSKLSLVTTQNYETD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVE+E+    +G+VEEIES   VVQEKIM WEADQSMIWDS   ++A EYLNAADEAR L
Sbjct: 61  VVEVEINERGVGDVEEIESSFNVVQEKIMAWEADQSMIWDSTTLNEACEYLNAADEAREL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KL+ LCLSKDE  +EL RKAHDVLQTAMARLEEEFRHLLA+SSLE++ ES+S H  ED
Sbjct: 121 VGKLDSLCLSKDEYGYELLRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESMSFHVVED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
            V+DGS+ LY DESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQEC
Sbjct: 181 TVEDGSSSLYHDESFESSVRSSSVGRVLENSIIDLVNPDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQ YN LRRDALNECLLTLEMEK+SIEDVLKMDWVTLNSKIRKWNRAMKRFVR+YL SEK
Sbjct: 241 IQVYNLLRRDALNECLLTLEMEKLSIEDVLKMDWVTLNSKIRKWNRAMKRFVRIYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQIFGEEG VSL+CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEVVEE L
Sbjct: 301 SLCDQIFGEEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVVEEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
            DID LYCD++GY VRIEYHDV KSLG SVRATFLEF KAIAANTSP+PFAGGGIHHLTK
Sbjct: 361 FDIDTLYCDDIGYLVRIEYHDVLKSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDY +SLN LLKD ED+ P SPS S+N SRE + E        GEFSPMAR
Sbjct: 421 YVMNYLMILTDYRDSLNLLLKDDEDVCPNSPSSSLNPSREEDRE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL QIFGEDW+RKHY 
Sbjct: 481 HFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIQIFGEDWVRKHYK 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
           KFQQQA NYERASWNSIL YLRED    PGS S SKNVLK+RLRSFN+AFEEIYKTQT+W
Sbjct: 541 KFQQQATNYERASWNSILQYLREDGIQNPGSTSVSKNVLKDRLRSFNLAFEEIYKTQTAW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           IIHDSRLREDLRISTSLRVIHAYRAF+GRCNNHVSDKLIKY+PDDLEGYLLDLFEGSPKS
Sbjct: 601 IIHDSRLREDLRISTSLRVIHAYRAFYGRCNNHVSDKLIKYTPDDLEGYLLDLFEGSPKS 659

Query: 661 LPNTNRR 664
           L NT+RR
Sbjct: 661 LANTSRR 659

BLAST of MS014532 vs. ExPASy TrEMBL
Match: A0A1S3CI03 (Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103501191 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 6.2e-304
Identity = 540/667 (80.96%), Postives = 585/667 (87.71%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGEC CLVSE  GE++L+AAAN+IIKAL S+I ISDD KKVLADLC++LSLV ++N   D
Sbjct: 1   MGECECLVSESVGEERLVAAANYIIKALSSNIRISDDGKKVLADLCSKLSLVTTQNYETD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVE+E+    +G+VEEIES   VVQEKIM WEADQSMIWDS   ++A EYLNAADEAR L
Sbjct: 61  VVEVEINERGVGDVEEIESSFNVVQEKIMAWEADQSMIWDSTTLNEACEYLNAADEAREL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KL+ LCLSKDE  +EL RKAHDVLQTAMARLEEEFRHLLA+SSLE++ ES+S H  ED
Sbjct: 121 VGKLDSLCLSKDEYGYELLRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESMSFHVVED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
            V+DGS+ LY DESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQEC
Sbjct: 181 TVEDGSSSLYHDESFESSVRSSSVGRVLENSIIDLVNPDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQ YN LRRDALNECLLTLEMEK+SIEDVLKMDWVTLNSKIRKWNRAMKRFVR+YL SEK
Sbjct: 241 IQVYNLLRRDALNECLLTLEMEKLSIEDVLKMDWVTLNSKIRKWNRAMKRFVRIYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQIFGEEG VSL+CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEVVEE L
Sbjct: 301 SLCDQIFGEEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVVEEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
            DID LYCD++GY VRIEYHDV KSLG SVRATFLEF KAIAANTSP+PFAGGGIHHLTK
Sbjct: 361 FDIDTLYCDDIGYLVRIEYHDVLKSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDY +SLN LLKD ED+ P SPS S+N SRE + E        GEFSPMAR
Sbjct: 421 YVMNYLMILTDYRDSLNLLLKDDEDVCPNSPSSSLNPSREEDRE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL QIFGEDW+RKHY 
Sbjct: 481 HFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIQIFGEDWVRKHYK 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
           KFQQQA NYERASWNSIL YLRED    PGS S SKNVLK+RLRSFN+AFEEIYKTQT+W
Sbjct: 541 KFQQQATNYERASWNSILQYLREDGIQNPGSTSVSKNVLKDRLRSFNLAFEEIYKTQTAW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           IIHDSRLREDLRISTSLRVIHAYRAF+GRCNNHVSDKLIKY+PDDLEGYLLDLFEGSPKS
Sbjct: 601 IIHDSRLREDLRISTSLRVIHAYRAFYGRCNNHVSDKLIKYTPDDLEGYLLDLFEGSPKS 659

Query: 661 LPNTNRR 664
           L NT+RR
Sbjct: 661 LANTSRR 659

BLAST of MS014532 vs. ExPASy TrEMBL
Match: A0A0A0KXV3 (Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_4G101790 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.8e-303
Identity = 539/667 (80.81%), Postives = 586/667 (87.86%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MGEC CLVSE  GE++L+AAAN+IIKAL S+I ISDD KKVLADLC++LSLV ++N   D
Sbjct: 1   MGECECLVSESGGEERLVAAANYIIKALSSNIRISDDGKKVLADLCSKLSLVSTQNYETD 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           VVE+E+E   +G+VEE+ESR  VVQEKIM WEADQSMIWDS   ++A EYLNAADEA  L
Sbjct: 61  VVEVEIEERGVGDVEEVESRFNVVQEKIMAWEADQSMIWDSTTLNEACEYLNAADEAWDL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KL+ LCLSKDE  +EL RKAHDVLQTAMARLEEEFRHLLA+SSLE++ ES+S H  ED
Sbjct: 121 VGKLDSLCLSKDEYSYELLRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESMSFHVVED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
            V+DGST LYRDESFE+SVRSSSVGR LENSIIDLVNPDAV EL GIANVMFK+GYDQEC
Sbjct: 181 TVEDGSTSLYRDESFESSVRSSSVGRVLENSIIDLVNPDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQ YN LRRDALNECLLTLEMEK+SIEDVLKMDWVTLNSKIRKWNRAMKRFVR+YL SEK
Sbjct: 241 IQVYNLLRRDALNECLLTLEMEKLSIEDVLKMDWVTLNSKIRKWNRAMKRFVRIYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQIFGEEG VSL+CFVESSKASMLQ LNFGEAMAIGPHTPEKLNRILEMYEVVEE L
Sbjct: 301 SLCDQIFGEEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHTPEKLNRILEMYEVVEEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
            DID LYCD++GY VRIEYHDV KSLG SVRATFLEF KAIAANTSP+PFAGGGIHHLTK
Sbjct: 361 FDIDTLYCDDIGYLVRIEYHDVLKSLGQSVRATFLEFEKAIAANTSPNPFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYLMILTDY +SLN LLKD ED+ P SPS S+N +RE + E        GEFSPMAR
Sbjct: 421 YVMNYLMILTDYRDSLNLLLKDDEDVCPNSPSSSLNPTREEDRE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVA+ILE+NL+EKSKQYKD ALQ FFLMNNIHYMAQKVRGSEL +IFGEDW+RKHY 
Sbjct: 481 HFRSVASILESNLDEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELIRIFGEDWVRKHYK 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
           KFQQQA NYERASWNSIL YLRED     GS S SKNVLK+RLRSFN+AFEEIYKTQT+W
Sbjct: 541 KFQQQATNYERASWNSILQYLREDGIQNTGSTSVSKNVLKDRLRSFNLAFEEIYKTQTAW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           IIHDSRLREDLRISTSLRVIHAYRAF+GRCNNHVSDKLIKY+PDDLEGYLLDLFEGSPKS
Sbjct: 601 IIHDSRLREDLRISTSLRVIHAYRAFYGRCNNHVSDKLIKYTPDDLEGYLLDLFEGSPKS 659

Query: 661 LPNTNRR 664
           L NT+RR
Sbjct: 661 LANTSRR 659

BLAST of MS014532 vs. ExPASy TrEMBL
Match: A0A6J1G919 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111452073 PE=3 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 7.9e-291
Identity = 523/667 (78.41%), Postives = 576/667 (86.36%), Query Frame = 0

Query: 1   MGECGCLVSELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRAD 60
           MG+C  + S+L GE+QLIAAAN IIKAL S+I++SDD K V+ DLC++LSL+ ++N +  
Sbjct: 1   MGDCEFVGSDLVGEEQLIAAANCIIKALSSNISLSDDTKSVMVDLCSKLSLITTQNHQIG 60

Query: 61  VVEIEVEGTEIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGL 120
           V     +  ++G V+EIESRL VVQ+KIMGWEADQSMIWDSA P+DA +YLNAAD+A  L
Sbjct: 61  V----TKDRDVGLVDEIESRLNVVQKKIMGWEADQSMIWDSAFPNDACDYLNAADDAWEL 120

Query: 121 VSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTED 180
           V KLE LC  +DE  +ELSRKAHDVLQTAMARLEEEFRHLLA+SSLE++ ESVS H TED
Sbjct: 121 VGKLESLCF-EDEHGYELSRKAHDVLQTAMARLEEEFRHLLAKSSLEYEPESVSSHVTED 180

Query: 181 AVDDGSTHLYRDESFEASVRSSSVGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQEC 240
           +V++GST LYRDESFE+SVRS SVGR LENSIIDLVN DAV EL GIANVMFK+GYDQEC
Sbjct: 181 SVEEGSTSLYRDESFESSVRSCSVGRTLENSIIDLVNSDAVIELRGIANVMFKAGYDQEC 240

Query: 241 IQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEK 300
           IQAYNHLRR+ALNECL  LEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYL SEK
Sbjct: 241 IQAYNHLRREALNECLSMLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLASEK 300

Query: 301 CLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELL 360
            L DQ+FG+EG VSL+CFVESSKASMLQ LNFGEAMAIGPH PEKLNRILEMYEVV E L
Sbjct: 301 FLCDQVFGDEGLVSLSCFVESSKASMLQLLNFGEAMAIGPHPPEKLNRILEMYEVVGEHL 360

Query: 361 SDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTK 420
           SDID LYCD+VGY VRIEYHDV  SLG SV ATFLEFGKAIAANTSP+ FAGGGIHHLTK
Sbjct: 361 SDIDTLYCDDVGYLVRIEYHDVLNSLGKSVTATFLEFGKAIAANTSPNAFAGGGIHHLTK 420

Query: 421 YVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMAR 480
           YVMNYL+ILTDY +SLN LL DGED+YP SPS S N SRE E+E        GEFSPMAR
Sbjct: 421 YVMNYLLILTDYGDSLNVLLGDGEDVYPNSPSSSENPSREEEKE--------GEFSPMAR 480

Query: 481 HFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYG 540
           HFRSVATILE+NLEEKSKQYKD ALQ FFLMNNIHYMAQKVRGSELSQIFGEDW+RK Y 
Sbjct: 481 HFRSVATILESNLEEKSKQYKDPALQHFFLMNNIHYMAQKVRGSELSQIFGEDWVRKRYM 540

Query: 541 KFQQQAMNYERASWNSILSYLRED----PGSNSESKNVLKERLRSFNVAFEEIYKTQTSW 600
            FQQ  +NY+RASWNSI+ YL ED    PGSNS S+NVLKERLRSFNVAFEEIYKTQTSW
Sbjct: 541 NFQQHVLNYKRASWNSIVLYLMEDGVQHPGSNSVSRNVLKERLRSFNVAFEEIYKTQTSW 600

Query: 601 IIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGSPKS 660
           I+HDSRLR+DLRISTSL+VI AYR FFGRCNNHVSDKLIKY+PDDLEG+LLDLFEGSPKS
Sbjct: 601 IVHDSRLRDDLRISTSLQVIPAYRGFFGRCNNHVSDKLIKYTPDDLEGFLLDLFEGSPKS 654

Query: 661 LPNTNRR 664
           L NTNRR
Sbjct: 661 LANTNRR 654

BLAST of MS014532 vs. TAIR 10
Match: AT3G29400.1 (exocyst subunit exo70 family protein E1 )

HSP 1 Score: 589.3 bits (1518), Expect = 3.7e-168
Identity = 339/670 (50.60%), Postives = 437/670 (65.22%), Query Frame = 0

Query: 10  ELEGEKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGT 69
           E++GE++LI AA +++K L S  +++ +AK VL +L  ELS VV       + E   +  
Sbjct: 5   EVDGEEKLIVAAKYLVKELRSGKSLTKNAKNVLGNLLLELSRVV-------IAEDTQDRD 64

Query: 70  EIGEVEEIESRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCL 129
           E  E+ EIE RL VV EKIM  E D+SMIWD  + D+ + YL+A +E R L+ +L     
Sbjct: 65  EEDEIGEIEERLNVVSEKIMTREVDESMIWDLGS-DEGNLYLDAVNELRSLIDRL----- 124

Query: 130 SKDEGDFELS-RKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHSTE--DAVDDGS 189
              +G  ELS RKAHDVLQ AMARLE+EF+HLL  + L F+LE  S  S E    V++ S
Sbjct: 125 ---DGSEELSLRKAHDVLQIAMARLEDEFKHLLVENRLPFELEHSSFRSIEADHGVEEES 184

Query: 190 THLYRDESFEASVRSSS--VGRNLENSIIDLVNPDAVCELMGIANVMFKSGYDQECIQAY 249
              +   S E  +  S+    RN  + ++DLVNPD + +L  IAN M  SGYD+ECIQ  
Sbjct: 185 MASFGAASTEDLILGSNNDSRRNSGDVVVDLVNPDVILDLKNIANTMIASGYDRECIQVC 244

Query: 250 NHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSD 309
             +R+DAL+E L   E+EK+SIEDVL+MDW TLN+ I+KW R M+  V+VYL SEK L +
Sbjct: 245 TMVRKDALDEFLYNHEVEKLSIEDVLRMDWATLNTNIKKWVRVMRDIVQVYLLSEKSLDN 304

Query: 310 QIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDID 369
           QIFG+   + LTCFV++ KA M+Q LNFGEA+++GP  PEKL RILEMYE+  ELL +ID
Sbjct: 305 QIFGDLNEIGLTCFVDTVKAPMMQLLNFGEAVSLGPRQPEKLLRILEMYELASELLPEID 364

Query: 370 ALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMN 429
           AL+ D  G  VR EY +V + LG   R TFLEF  AIAA+ S HPF GG +H LT YVMN
Sbjct: 365 ALFLDHPGSSVRTEYREVMRRLGDCARTTFLEFKSAIAADVSSHPFPGGAVHPLTNYVMN 424

Query: 430 YLMILTDYSESLNFLLKDGEDM--YPISPSCSMNSSREREEESESRGSCSGE-FSPMARH 489
           YLM LTD+  +L+ LL + +D     I PS  + +    EEES    S S E F  M RH
Sbjct: 425 YLMALTDFKHTLDSLLMEHDDAEDLTIPPSPDIINPVMVEEESTYENSSSPEKFLAMTRH 484

Query: 490 FRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGK 549
           F S+ ++LE NL+EKSK YKD +LQ  FL+NNIHYM +KV  SEL  IFG+ W RKH  K
Sbjct: 485 FYSITSVLEANLQEKSKLYKDVSLQHIFLLNNIHYMTRKVLKSELRHIFGDKWNRKHTWK 544

Query: 550 FQQQAMNYERASWNSILSYLRED-------PGSNSESKNVL-KERLRSFNVAFEEIYKTQ 609
           FQQQA  YERA+W  +LS+L++D        GS S SKN+  +ER + FN AFEE+YK Q
Sbjct: 545 FQQQATEYERATWLPVLSFLKDDGSGSGPGSGSGSGSKNLRPRERFQGFNTAFEEVYKAQ 604

Query: 610 TSWIIHDSRLREDLRISTSLRVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFEGS 664
           T W+I D  LRED+R   S+ VI AY  F+ R  N VS++ IKY+ DD+E  LLDLF GS
Sbjct: 605 TGWLISDEGLREDVRTKASMWVIQAYWTFYSRHKNSVSERYIKYTTDDIERLLLDLFAGS 658

BLAST of MS014532 vs. TAIR 10
Match: AT5G61010.1 (exocyst subunit exo70 family protein E2 )

HSP 1 Score: 436.8 bits (1122), Expect = 3.1e-122
Identity = 263/652 (40.34%), Postives = 386/652 (59.20%), Query Frame = 0

Query: 20  AANFIIKAL-GSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIGEVEEIE 79
           A + ++KAL  SD  +  + +K+L+DL   LS          + + +VE        EI+
Sbjct: 20  ACHHVVKALRASDNNLDANLRKLLSDLEMHLSTF-------GIADTKVEDAGF---SEIK 79

Query: 80  SRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKDEGDFEL 139
            R K   ++I  WE +QS +++ A   +A ++  A  + + ++   + L +  ++ + ++
Sbjct: 80  KRFKEAVKRIRSWETNQSTMFE-AGLSEADQFFQALYDVQTVLVGFKALPMKTNQMEKDV 139

Query: 140 SRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHS-TEDAVDDGSTHLYRDESF-E 199
             +A   L  AM RLE+E   +L +     Q + ++V S  +D V D S     DE   E
Sbjct: 140 YNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVSLDDEVIVE 199

Query: 200 ASVRSSSVG-RNLENSIIDLVNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRDALNEC 259
           AS         +  NS  DLV+P  +  +  IAN MF   YDQ   +A+  ++R+AL E 
Sbjct: 200 ASSHEDDEQISDFYNS--DLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQREALEEY 259

Query: 260 LLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSL 319
           ++TLEME+ S  DVL+MDW  LN  +RKW + +K   +VYL SEK L DQI G+  S+S 
Sbjct: 260 MVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGDFESIST 319

Query: 320 TCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHV 379
            CF+E SK ++L  LNFGEA+ +    PE L R L MYEV  E+L D+D L+ DE G  +
Sbjct: 320 ACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPDETGSSL 379

Query: 380 RIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSES 439
           RI +H++ K L      TFL+F  AIA++ S  PF GGGIHHLT+YVMNYL +L +Y++S
Sbjct: 380 RIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLKLLPEYTDS 439

Query: 440 LNFLLKD--GEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHFRSVATILENNL 499
           LN LL++   +D  P               E          FSPMARH RS+ T LE++L
Sbjct: 440 LNSLLQNIHVDDSIP---------------EKTGEDVLPSTFSPMARHLRSIVTTLESSL 499

Query: 500 EEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKFQQQAMNYERAS 559
           E K++ Y D AL+  FLMNN  YM QKV+GSEL ++FG++WIRKH   +Q    NYER++
Sbjct: 500 ERKAQLYADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERST 559

Query: 560 WNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSL 619
           W+SIL+ LR+    N++S   L+ER R F++AF+++YK QT W + DS LR+DL ISTS+
Sbjct: 560 WSSILALLRD----NNDSVRTLRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSV 619

Query: 620 RVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFE--GSPKSLPNTNRR 664
           +V+ +YR F GR    + +K I+Y+ +D+E  LLDLFE   SP+SL ++ +R
Sbjct: 620 KVVQSYRGFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of MS014532 vs. TAIR 10
Match: AT5G61010.2 (exocyst subunit exo70 family protein E2 )

HSP 1 Score: 436.8 bits (1122), Expect = 3.1e-122
Identity = 263/652 (40.34%), Postives = 386/652 (59.20%), Query Frame = 0

Query: 20  AANFIIKAL-GSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIGEVEEIE 79
           A + ++KAL  SD  +  + +K+L+DL   LS          + + +VE        EI+
Sbjct: 20  ACHHVVKALRASDNNLDANLRKLLSDLEMHLSTF-------GIADTKVEDAGF---SEIK 79

Query: 80  SRLKVVQEKIMGWEADQSMIWDSAAPDDASEYLNAADEARGLVSKLEGLCLSKDEGDFEL 139
            R K   ++I  WE +QS +++ A   +A ++  A  + + ++   + L +  ++ + ++
Sbjct: 80  KRFKEAVKRIRSWETNQSTMFE-AGLSEADQFFQALYDVQTVLVGFKALPMKTNQMEKDV 139

Query: 140 SRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHS-TEDAVDDGSTHLYRDESF-E 199
             +A   L  AM RLE+E   +L +     Q + ++V S  +D V D S     DE   E
Sbjct: 140 YNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVSLDDEVIVE 199

Query: 200 ASVRSSSVG-RNLENSIIDLVNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRDALNEC 259
           AS         +  NS  DLV+P  +  +  IAN MF   YDQ   +A+  ++R+AL E 
Sbjct: 200 ASSHEDDEQISDFYNS--DLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQREALEEY 259

Query: 260 LLTLEMEKMSIEDVLKMDWVTLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSL 319
           ++TLEME+ S  DVL+MDW  LN  +RKW + +K   +VYL SEK L DQI G+  S+S 
Sbjct: 260 MVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGDFESIST 319

Query: 320 TCFVESSKASMLQFLNFGEAMAIGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHV 379
            CF+E SK ++L  LNFGEA+ +    PE L R L MYEV  E+L D+D L+ DE G  +
Sbjct: 320 ACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPDETGSSL 379

Query: 380 RIEYHDVQKSLGHSVRATFLEFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSES 439
           RI +H++ K L      TFL+F  AIA++ S  PF GGGIHHLT+YVMNYL +L +Y++S
Sbjct: 380 RIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLKLLPEYTDS 439

Query: 440 LNFLLKD--GEDMYPISPSCSMNSSREREEESESRGSCSGEFSPMARHFRSVATILENNL 499
           LN LL++   +D  P               E          FSPMARH RS+ T LE++L
Sbjct: 440 LNSLLQNIHVDDSIP---------------EKTGEDVLPSTFSPMARHLRSIVTTLESSL 499

Query: 500 EEKSKQYKDAALQQFFLMNNIHYMAQKVRGSELSQIFGEDWIRKHYGKFQQQAMNYERAS 559
           E K++ Y D AL+  FLMNN  YM QKV+GSEL ++FG++WIRKH   +Q    NYER++
Sbjct: 500 ERKAQLYADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERST 559

Query: 560 WNSILSYLREDPGSNSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSL 619
           W+SIL+ LR+    N++S   L+ER R F++AF+++YK QT W + DS LR+DL ISTS+
Sbjct: 560 WSSILALLRD----NNDSVRTLRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSV 619

Query: 620 RVIHAYRAFFGRCNNHVSDKLIKYSPDDLEGYLLDLFE--GSPKSLPNTNRR 664
           +V+ +YR F GR    + +K I+Y+ +D+E  LLDLFE   SP+SL ++ +R
Sbjct: 620 KVVQSYRGFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of MS014532 vs. TAIR 10
Match: AT5G50380.1 (exocyst subunit exo70 family protein F1 )

HSP 1 Score: 402.5 bits (1033), Expect = 6.5e-112
Identity = 255/701 (36.38%), Postives = 392/701 (55.92%), Query Frame = 0

Query: 14  EKQLIAAANFIIKALGSDITISDDAKKVLADLCTELSLVVSRNQRADVVEIEVEGTEIGE 73
           E +++A A  I+K+L +   + +D           L +  S + R   ++  +   E  +
Sbjct: 23  EDRVLATAQQIMKSLNTPKEVREDM----------LLIFSSFDNRLSNIKTAMTNQEENQ 82

Query: 74  VEEIESRLKVVQEKIMGWEADQ------------------SMIWDSAAPDDASEYLNAAD 133
            + + +RL+  +  I  W+                     S+ +D  +P++A+E+L+A D
Sbjct: 83  NDALVARLEAAESVIHRWDGGNDSSRHSSSSSGNYRSSSFSLSFDE-SPEEATEFLSAVD 142

Query: 134 EARGLVSKLEGLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLE---- 193
           E   ++S LE L     E   ++  +A   LQ AM++LE+EFR +L R+++    E    
Sbjct: 143 E---IISLLEDL---SSENKPDMVDRADSALQMAMSQLEDEFRRILIRNTVPLDAERLYG 202

Query: 194 -----SVSVHSTEDAVDDGSTHLYRDESFEAS------VRSSSVGRNLENSIIDLVNPDA 253
                S+S    +   D  +  L  D     S       R  S+G +L    +DL+NP A
Sbjct: 203 SMRRVSLSFADGDVVEDFENFGLVADGDGSGSRRRLFHERGGSIGCDL---WVDLINPTA 262

Query: 254 VCELMGIANVMFKSGYDQECIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWVTLNSK 313
           V +L  IA  M ++GY++EC+Q Y+ +RRDAL++CL+ L +EK+SIE+V K+DW +++ K
Sbjct: 263 VEDLKEIAERMIRAGYEKECVQVYSSVRRDALDDCLMILGVEKLSIEEVQKIDWKSMDEK 322

Query: 314 IRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEAMAIGP 373
           ++KW +A+K  VRV L  EK + D+IF    S    CF E++K+ ++Q LNFGEA+AIG 
Sbjct: 323 MKKWIQAVKITVRVLLVGEKKICDEIFSSSESSKEVCFNETTKSCVMQLLNFGEAVAIGR 382

Query: 374 HTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFLEFGKA 433
            + EKL RIL+MY+ +  +L  ++ +  D     V  E   V ++LG + R TF+EF   
Sbjct: 383 RSSEKLFRILDMYDALANVLQTLEVMVTD---CFVCNETKGVLEALGDAARGTFVEFENN 442

Query: 434 IAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSMNSSRE 493
           +   TS  P   G +H + +YVMNY+ ++ DY+ +LN LL+  E     S   S + S E
Sbjct: 443 VRNETSKRPTTNGEVHPMIRYVMNYMKLIVDYAVTLNSLLESNE-----SSGVSGDDSTE 502

Query: 494 REEESESRGSCSGEFSPMARHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIHYMAQK 553
                        E SP+A+    + T LE+NLE+KSK Y+D  LQ  F+MNNI+Y+ QK
Sbjct: 503 -------------EMSPLAKRILGLITSLESNLEDKSKLYEDGGLQHVFMMNNIYYIVQK 562

Query: 554 VRGSELSQIFGEDWIRKHYGKFQQQAMNYERASWNSILSYLRED-------------PGS 613
           V+ SEL ++ G+DW+RK  G+ +Q A  Y RASW+ +LS LR++               S
Sbjct: 563 VKDSELGKLLGDDWVRKRRGQIRQYATGYLRASWSRVLSALRDESMGGSSSGSPSYGQRS 622

Query: 614 NSESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSLRVIHAYRAFFGRCN 664
           N+ SK  LKER R FN +FEE+Y+ QT+W + D +LRE+LRIS S +VI AYRAFFGR  
Sbjct: 623 NNSSKMALKERFRGFNASFEELYRLQTAWKVPDPQLREELRISISEKVIPAYRAFFGRNR 682

BLAST of MS014532 vs. TAIR 10
Match: AT1G54090.1 (exocyst subunit exo70 family protein D2 )

HSP 1 Score: 339.3 bits (869), Expect = 6.7e-93
Identity = 214/629 (34.02%), Postives = 338/629 (53.74%), Query Frame = 0

Query: 73  EVEEIESRLKVVQEKIMGW------EADQSMIWDSAAP-DDASEYLNAADEARGLVSKLE 132
           E   ++S L+  ++ I+ W      EA +++I+ S    ++   YL A DE +  +S + 
Sbjct: 5   ETRGVDSDLETAEKIILRWDSTTSEEAKENLIFQSGGDREEVDRYLKAVDEVQRHISSI- 64

Query: 133 GLCLSKDEGDFELSRKAHDVLQTAMARLEEEFRHLLARSSLEFQLESVSVHST------- 192
                       +S +    +Q AMARLE+E R++L   +  F+ +S+ + S+       
Sbjct: 65  -----------SISDEVKATIQIAMARLEDELRNILISQTSTFEPDSLLLDSSLSSSSFA 124

Query: 193 --------EDAVDDGSTHLYRDE----------SFEASVRSSSVGRNLENSI-----IDL 252
                   +D  DDG+    + +          S   S R S   R+   S      IDL
Sbjct: 125 SSSRTELEDDTCDDGNEEEEQQQVDLVLPDGSGSDSGSRRLSRSRRSNSKSTSSIREIDL 184

Query: 253 VNPDAVCELMGIANVMFKSGYDQECIQAYNHLRRDALNECLLTLEMEKMSIEDVLKMDWV 312
           V P+AV +L  IA  M  +GY +EC+Q Y ++R+ A+      L + K+ I DV ++DW 
Sbjct: 185 VTPEAVSDLRSIAQRMIGAGYSRECVQVYGNVRKSAMEMIFKQLGIVKLGIGDVQRLDWE 244

Query: 313 TLNSKIRKWNRAMKRFVRVYLPSEKCLSDQIFGEEGSVSLTCFVESSKASMLQFLNFGEA 372
            +  KIR+W RA K  VRV   SEK L +QIF  EG++  TCF+E  K S LQ  NF EA
Sbjct: 245 AVEVKIRRWIRAAKVCVRVVFASEKRLCEQIF--EGTMEETCFMEIVKTSALQLFNFPEA 304

Query: 373 MAIGPHTPEKLNRILEMYEVVEELLSDIDALYCDEVGYHVRIEYHDVQKSLGHSVRATFL 432
           ++I   +PEKL +IL++++ + +LL D++ ++       + ++  ++Q  L  + R    
Sbjct: 305 ISISRRSPEKLFKILDLHDAITDLLPDMEEIFDSSSSESILVQATEIQSRLAEAARGILT 364

Query: 433 EFGKAIAANTSPHPFAGGGIHHLTKYVMNYLMILTDYSESLNFLLKDGEDMYPISPSCSM 492
           EF  A+    S  P  GG IH LT+YVMNYL +++DY E+L        D+    P   +
Sbjct: 365 EFENAVFREPSVVPVPGGTIHPLTRYVMNYLNLISDYRETL-------IDLVMTKPCRGL 424

Query: 493 NSSREREEESESRGSCSGEFSPMARHFRSVATILENNLEEKSKQYKDAALQQFFLMNNIH 552
             + +R +         G  SP+A H      +L+ NLEEKS  Y+D  L   F+MNN+H
Sbjct: 425 KCTNDRNDPDMDISELEG-ISPLALHMIWTMVMLQFNLEEKSLHYRDEPLSHIFVMNNVH 484

Query: 553 YMAQKVRGS-ELSQIFGEDWIRKHYGKFQQQAMNYERASWNSILSYLRED------PGSN 612
           Y+ QKV+ S EL ++ G+ ++RK  G F+Q A  Y+RA+W  +L+ LR++        S+
Sbjct: 485 YIVQKVKSSPELMELIGDKYLRKLTGIFRQAATKYQRATWVRVLNSLRDEGLHVSGSFSS 544

Query: 613 SESKNVLKERLRSFNVAFEEIYKTQTSWIIHDSRLREDLRISTSLRVIHAYRAFFGRCNN 653
             SK+ L+ER ++FN  FEE+++ Q++W + D++LRE+LRIS S  +I AYR+F GR   
Sbjct: 545 GVSKSALRERFKAFNTMFEEVHRIQSTWSVPDTQLREELRISLSEHLIPAYRSFLGRFRG 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155076.10.0e+0099.40exocyst complex component EXO70E2 [Momordica charantia][more]
XP_038897591.11.2e-30681.59exocyst complex component EXO70E2 [Benincasa hispida][more]
XP_008462929.11.3e-30380.96PREDICTED: exocyst complex component EXO70B1 [Cucumis melo] >KAA0061338.1 exocys... [more]
XP_011653366.13.7e-30380.81exocyst complex component EXO70E2 [Cucumis sativus] >KGN53679.1 hypothetical pro... [more]
KAG7036789.15.6e-29178.56Exocyst complex component EXO70B1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9FNR34.4e-12140.34Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=... [more]
Q9FGH91.2e-8331.56Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LZD35.1e-6930.97Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
Q9LMJ44.3e-6828.57Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
Q8VY275.8e-5727.08Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1DQM10.0e+0099.40Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5A7V1A06.2e-30480.96Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CI036.2e-30480.96Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103501191 PE=... [more]
A0A0A0KXV31.8e-30380.81Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_4G101790 ... [more]
A0A6J1G9197.9e-29178.41Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114520... [more]
Match NameE-valueIdentityDescription
AT3G29400.13.7e-16850.60exocyst subunit exo70 family protein E1 [more]
AT5G61010.13.1e-12240.34exocyst subunit exo70 family protein E2 [more]
AT5G61010.23.1e-12240.34exocyst subunit exo70 family protein E2 [more]
AT5G50380.16.5e-11236.38exocyst subunit exo70 family protein F1 [more]
AT1G54090.16.7e-9334.02exocyst subunit exo70 family protein D2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availableCOILSCoilCoilcoord: 486..506
NoneNo IPR availableCOILSCoilCoilcoord: 74..94
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 91..660
e-value: 9.3E-158
score: 528.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..473
NoneNo IPR availablePANTHERPTHR12542:SF49EXOCYST SUBUNIT EXO70 FAMILY PROTEINcoord: 24..657
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 283..647
e-value: 3.3E-112
score: 375.2
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 24..657
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 95..651

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014532.1MS014532.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
cellular_component GO:0000145 exocyst