MS014522 (gene) Bitter gourd (TR) v1

Overview


Name	MS014522
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cytokinin dehydrogenase
Location	scaffold553: 585079 .. 585260 (-)
RNA-Seq Expression	MS014522
Synteny	MS014522

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTACATTCCCAAGTCAATTTTCACAACGGACCTGGGGTACGTTGACTTCCTAGACCGGGTTCACAAGGGCGAGCTGAATCTCTTCATCCTGAAAGGATCGGCAACTTTGACCAAGGAGTCTTCAAGGGCATTTTGGGAAACAACACCAGTGGGTCCATCCTCACTACCCCATTAACAAGG

mRNA sequence

AATTACATTCCCAAGTCAATTTTCACAACGGACCTGGGGTACGTTGACTTCCTAGACCGGGTTCACAAGGGCGAGCTGAATCTCTTCCCTGAAAGGATCGGCAACTTTGACCAAGGAGTCTTCAAGGGCATTTTGGGAAACAACACCAGTGGGTCCATCCTCACTACCCCATTAACAAGG

Coding sequence (CDS)

AATTACATTCCCAAGTCAATTTTCACAACGGACCTGGGGTACGTTGACTTCCTAGACCGGGTTCACAAGGGCGAGCTGAATCTCTTCCCTGAAAGGATCGGCAACTTTGACCAAGGAGTCTTCAAGGGCATTTTGGGAAACAACACCAGTGGGTCCATCCTCACTACCCCATTAACAAGG

Protein sequence

NYIPKSIFTTDLGYVDFLDRVHKGELNLFPERIGNFDQGVFKGILGNNTSGSILTTPLTR

Homology

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BLAST of MS014522 vs. NCBI nr
Match: XP_011649640.1 (cytokinin dehydrogenase 5 [Cucumis sativus] >KGN62850.2 hypothetical protein Csa_021914 [Cucumis sativus])

HSP 1 Score: 87.8 bits (216), Expect = 3.4e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 356 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 415

[Close]

BLAST of MS014522 vs. NCBI nr
Match: XP_008444755.1 (PREDICTED: cytokinin dehydrogenase 5 [Cucumis melo])

HSP 1 Score: 87.8 bits (216), Expect = 3.4e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 357 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 416

[Close]

BLAST of MS014522 vs. NCBI nr
Match: KAA0065202.1 (cytokinin dehydrogenase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 87.8 bits (216), Expect = 3.4e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 357 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 416

[Close]

BLAST of MS014522 vs. NCBI nr
Match: XP_038886192.1 (LOW QUALITY PROTEIN: cytokinin dehydrogenase 5-like [Benincasa hispida])

HSP 1 Score: 87.8 bits (216), Expect = 3.4e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 322 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 381

[Close]

BLAST of MS014522 vs. NCBI nr
Match: XP_022144150.1 (cytokinin dehydrogenase 5-like, partial [Momordica charantia])

HSP 1 Score: 87.0 bits (214), Expect = 5.9e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           +YIP S+FTTDLGYVDFLDRVHK E               LNLF    RIG+FD+GVFKG
Sbjct: 193 DYIPGSVFTTDLGYVDFLDRVHKAELKLRSKGLWEVPHPWLNLFVPKSRIGDFDRGVFKG 252

[Close]

BLAST of MS014522 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.4e-15
Identity = 43/77 (55.84%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           N+IP S+FTTDL YVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 343 NFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKGVFKG 402

[Close]

BLAST of MS014522 vs. ExPASy Swiss-Prot
Match: Q5ZAY9 (Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.7e-12
Identity = 39/78 (50.00%), Postives = 49/78 (62.82%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           N+IP ++FTTDL YVDFLDRVHK E               LNLF    RI +FD+GVF+G
Sbjct: 346 NFIPGTVFTTDLPYVDFLDRVHKAELKLRGKGMWEVPHPWLNLFVPASRIADFDRGVFRG 405

[Close]

BLAST of MS014522 vs. ExPASy Swiss-Prot
Match: Q9LTS3 (Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.0e-05
Identity = 29/79 (36.71%), Postives = 41/79 (51.90%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGELNLFPE-----------------RIGNFDQGVFKG 60
           N++   ++  D+ Y+DFL+RV  GELNL  +                 +I  FD GVFKG
Sbjct: 343 NHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKG 402

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BLAST of MS014522 vs. ExPASy Swiss-Prot
Match: Q9T0N8 (Cytokinin dehydrogenase 1 OS=Zea mays OX=4577 GN=CKX1 PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 1.5e-04
Identity = 29/78 (37.18%), Postives = 39/78 (50.00%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           +Y+    F  D+ Y  FLDRVH  E               LN+F    RI +FD+GVFKG
Sbjct: 358 SYVEGFAFQRDVAYAAFLDRVHGEEVALNKLGLWRVPHPWLNMFVPRSRIADFDRGVFKG 417

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BLAST of MS014522 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.0e-04
Identity = 27/77 (35.06%), Postives = 39/77 (50.65%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP ++F++++ Y++FLDRVH  E               LNL      I  F   VF  
Sbjct: 362 NYIPSTLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNN 421

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BLAST of MS014522 vs. ExPASy TrEMBL
Match: A0A5A7VA54 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005390 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 357 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 416

[Close]

BLAST of MS014522 vs. ExPASy TrEMBL
Match: A0A0A0LPC8 (Cytokinin dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_2G362450 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 356 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 415

[Close]

BLAST of MS014522 vs. ExPASy TrEMBL
Match: A0A1S3BBY3 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488003 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP+S+FTTDLGYVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 357 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 416

[Close]

BLAST of MS014522 vs. ExPASy TrEMBL
Match: A0A6J1CQU7 (cytokinin dehydrogenase 5-like OS=Momordica charantia OX=3673 GN=LOC111013907 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.8e-14
Identity = 46/77 (59.74%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           +YIP S+FTTDLGYVDFLDRVHK E               LNLF    RIG+FD+GVFKG
Sbjct: 193 DYIPGSVFTTDLGYVDFLDRVHKAELKLRSKGLWEVPHPWLNLFVPKSRIGDFDRGVFKG 252

[Close]

BLAST of MS014522 vs. ExPASy TrEMBL
Match: A0A6J1KNJ0 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111496174 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.1e-13
Identity = 44/77 (57.14%), Postives = 51/77 (66.23%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           +YIP+S+FTTDLGYVDFLDRVH  E               LNLF    RI +FD+GVFKG
Sbjct: 382 DYIPESVFTTDLGYVDFLDRVHTAELKLQSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 441

[Close]

BLAST of MS014522 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 80.1 bits (196), Expect = 6.7e-16
Identity = 43/77 (55.84%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           N+IP S+FTTDL YVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 343 NFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKGVFKG 402

[Close]

BLAST of MS014522 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 80.1 bits (196), Expect = 6.7e-16
Identity = 43/77 (55.84%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           N+IP S+FTTDL YVDFLDRVHK E               LNLF    RI +FD+GVFKG
Sbjct: 340 NFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKGVFKG 399

[Close]

BLAST of MS014522 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 50.1 bits (118), Expect = 7.4e-07
Identity = 29/79 (36.71%), Postives = 41/79 (51.90%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGELNLFPE-----------------RIGNFDQGVFKG 60
           N++   ++  D+ Y+DFL+RV  GELNL  +                 +I  FD GVFKG
Sbjct: 343 NHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKG 402

[Close]

BLAST of MS014522 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 45.8 bits (107), Expect = 1.4e-05
Identity = 27/77 (35.06%), Postives = 39/77 (50.65%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLF--PERIGNFDQGVFKG 60
           NYIP ++F++++ Y++FLDRVH  E               LNL      I  F   VF  
Sbjct: 362 NYIPSTLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNN 421

[Close]

BLAST of MS014522 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 44.7 bits (104), Expect = 3.1e-05
Identity = 25/77 (32.47%), Postives = 39/77 (50.65%), Query Frame = 0


Query: 1   NYIPKSIFTTDLGYVDFLDRVHKGE---------------LNLFPER--IGNFDQGVFKG 60
           +Y+  ++FTT++ Y  FLDRVH  E               LNL   R  I  F +GVF  
Sbjct: 349 SYVTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGN 408

The following BLAST results are available for this feature:

BLAST of MS014522 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649640.1	3.4e-14	59.74	cytokinin dehydrogenase 5 [Cucumis sativus] >KGN62850.2 hypothetical protein Csa...	[more]
XP_008444755.1	3.4e-14	59.74	PREDICTED: cytokinin dehydrogenase 5 [Cucumis melo]	[more]
KAA0065202.1	3.4e-14	59.74	cytokinin dehydrogenase 5 [Cucumis melo var. makuwa]	[more]
XP_038886192.1	3.4e-14	59.74	LOW QUALITY PROTEIN: cytokinin dehydrogenase 5-like [Benincasa hispida]	[more]
XP_022144150.1	5.9e-14	59.74	cytokinin dehydrogenase 5-like, partial [Momordica charantia]	[more]

BLAST of MS014522 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67YU0	9.4e-15	55.84	Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1	[more]
Q5ZAY9	5.7e-12	50.00	Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 ...	[more]
Q9LTS3	1.0e-05	36.71	Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1	[more]
Q9T0N8	1.5e-04	37.18	Cytokinin dehydrogenase 1 OS=Zea mays OX=4577 GN=CKX1 PE=1 SV=2	[more]
O22213	2.0e-04	35.06	Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1	[more]

BLAST of MS014522 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VA54	1.7e-14	59.74	Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A0A0LPC8	1.7e-14	59.74	Cytokinin dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_2G362450 PE=3 SV=1	[more]
A0A1S3BBY3	1.7e-14	59.74	Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488003 PE=3 SV=1	[more]
A0A6J1CQU7	2.8e-14	59.74	cytokinin dehydrogenase 5-like OS=Momordica charantia OX=3673 GN=LOC111013907 PE...	[more]
A0A6J1KNJ0	3.1e-13	57.14	Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111496174 PE=3 SV=1	[more]

BLAST of MS014522 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75450.1	6.7e-16	55.84	cytokinin oxidase 5	[more]
AT1G75450.2	6.7e-16	55.84	cytokinin oxidase 5	[more]
AT5G56970.1	7.4e-07	36.71	cytokinin oxidase 3	[more]
AT2G41510.1	1.4e-05	35.06	cytokinin oxidase/dehydrogenase 1	[more]
AT3G63440.1	3.1e-05	32.47	cytokinin oxidase/dehydrogenase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015345	Cytokinin dehydrogenase 1, FAD/cytokinin binding domain	PFAM	PF09265	Cytokin-bind	coord: 1..60 e-value: 3.5E-7 score: 29.9
IPR016170	Cytokinin dehydrogenase, C-terminal domain superfamily	GENE3D	3.40.462.10		coord: 1..60 e-value: 3.1E-7 score: 32.0
None	No IPR available	PANTHER	PTHR13878:SF143	CYTOKININ OXIDASE 5-RELATED	coord: 1..59
None	No IPR available	PANTHER	PTHR13878	GULONOLACTONE OXIDASE	coord: 1..59
IPR016164	FAD-linked oxidase-like, C-terminal	SUPERFAMILY	55103	FAD-linked oxidases, C-terminal domain	coord: 1..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014522.1	MS014522.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009690	cytokinin metabolic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0019139	cytokinin dehydrogenase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding