MS014516 (gene) Bitter gourd (TR) v1

BLAST of MS014516 vs. NCBI nr
Match: XP_022144443.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.2e-298
Identity = 527/528 (99.81%), Postives = 527/528 (99.81%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS
Sbjct: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. NCBI nr
Match: XP_023527776.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1009.6 bits (2609), Expect = 9.9e-291
Identity = 514/528 (97.35%), Postives = 519/528 (98.30%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMK LLTLAR S RRSSASFS  +RSASTSPAVA+SSPPPSPPPPNAMIYDRLAEAVKS
Sbjct: 1   MAMKRLLTLARTSPRRSSASFSQDVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVP ALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPNALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQF+IAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFSIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELA+RVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAERVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYE TKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYEMTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. NCBI nr
Match: XP_022998263.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.7e-290
Identity = 510/528 (96.59%), Postives = 521/528 (98.67%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MA+K+LLTLAR SHRR SASFS A+RSASTSPAVA+SSPPPSPPPPNAMIYDRLAE VKS
Sbjct: 1   MAIKHLLTLARTSHRRFSASFSRAVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEGVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETR+TTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRITTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTE+RTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTERRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVPKALDILADILQNSKF+EHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPKALDILADILQNSKFEEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVK LFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKNLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTA+QLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTATQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYETTKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYETTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVD+STIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDSSTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. NCBI nr
Match: XP_022930581.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 1007.7 bits (2604), Expect = 3.8e-290
Identity = 512/528 (96.97%), Postives = 519/528 (98.30%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMK LLTLAR S RRSSASFS  +RSASTSPAVA+SSPPPSPPPPNAM+YDRLAEAVKS
Sbjct: 1   MAMKRLLTLARTSPRRSSASFSQDVRSASTSPAVASSSPPPSPPPPNAMVYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WID+GSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDSGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVP ALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPHALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQF+IAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFSIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYE TKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYEMTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. NCBI nr
Match: KAG7018162.1 (putative mitochondrial-processing peptidase subunit beta, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1006.9 bits (2602), Expect = 6.4e-290
Identity = 513/528 (97.16%), Postives = 519/528 (98.30%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMK LLTLAR S RRSSASFS  +RSASTSPAVA+SSPPPSPPPPNAMIYDRLAEAVKS
Sbjct: 1   MAMKRLLTLARTSPRRSSASFSQDVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVP ALDILADILQNSKFDE+RISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPNALDILADILQNSKFDENRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQF+IAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFSIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYE TKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYEMTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. ExPASy Swiss-Prot
Match: Q42290 (Probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPbeta PE=1 SV=2)

HSP 1 Score: 840.9 bits (2171), Expect = 8.0e-243
Identity = 420/531 (79.10%), Postives = 477/531 (89.83%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRR---SSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEA 60
           MAMKNLL+LAR S RR   + A+ S +  SA  S   +AS    SPPPP+ M YD  AE 
Sbjct: 1   MAMKNLLSLARRSQRRLFLTQATRSSSSFSAIDSVPASASPTALSPPPPHLMPYDHAAEI 60

Query: 61  VKSKLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTAT 120
           +K+K+K+ ENPD RFLKY SPHP +  H  ILSAPETRVTTLPNGLRVATESNL+A+TAT
Sbjct: 61  IKNKIKKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTAT 120

Query: 121 VGVWIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTT 180
           VGVWIDAGSRFE++ETNGTAHFLEHMIFKGT++RT R LEEEIE++GGHLNAYTSREQTT
Sbjct: 121 VGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTT 180

Query: 181 YYAKVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHAT 240
           YYAKVLD +V +ALD+LADILQNSKF+E RI+RERDVILREM+EVEGQT+EV+ DHLHAT
Sbjct: 181 YYAKVLDSNVNQALDVLADILQNSKFEEQRINRERDVILREMQEVEGQTDEVVLDHLHAT 240

Query: 241 AFQYTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLF 300
           AFQYTPLGRTILGPAQN+++IT++ LQ+YI+THYTA RMVIAA+GAVKHE+ VEQVKKLF
Sbjct: 241 AFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLF 300

Query: 301 TKLSSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQA 360
           TKLSS+PTT SQLVA EPA FTGSEVR+IDDD+PLAQFA+AF GASWTDPDS+ALMVMQ 
Sbjct: 301 TKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQT 360

Query: 361 MLGSWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLA 420
           MLGSWNK+ GGGKH+GS+L QRVAINE+AES+MAFNTNYKDTGLFGVYAVAK DCLDDL+
Sbjct: 361 MLGSWNKNVGGGKHVGSDLTQRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLS 420

Query: 421 YAIMYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAEL 480
           YAIMYE TKLAYRVS+ DVTRARNQLKSSLLLH+DGTSP+AEDIGRQLLTYGRRIP AEL
Sbjct: 421 YAIMYEVTKLAYRVSDADVTRARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAEL 480

Query: 481 FARIDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           FARIDAVDAST+KRVAN++IYD+D+AI+A+GPIQ LPDYN FRRRTYWNRY
Sbjct: 481 FARIDAVDASTVKRVANKYIYDKDIAISAIGPIQDLPDYNKFRRRTYWNRY 531

BLAST of MS014516 vs. ExPASy Swiss-Prot
Match: Q3SZ71 (Mitochondrial-processing peptidase subunit beta OS=Bos taurus OX=9913 GN=PMPCB PE=2 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 3.7e-131
Identity = 233/438 (53.20%), Postives = 309/438 (70.55%), Query Frame = 0

Query: 88  ILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETEETNGTAHFLEHMIFKG 147
           +L+ PETRVT L NGLRVA+E +  A T TVG+WIDAGSR+E E+ NGTAHFLEHM FKG
Sbjct: 53  VLNVPETRVTCLENGLRVASEDSGLA-TCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKG 112

Query: 148 TEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKALDILADILQNSKFDEHR 207
           T+KR+   LE EIENMG HLNAYTSREQT YYAK   KD+P+A++ILADI+QNS   E  
Sbjct: 113 TKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAE 172

Query: 208 ISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGPAQNIRTITKDHLQSYI 267
           I RER VILREM+EVE   +EV+FD+LHATA+Q T LGRTILGP +NI++I +  L  YI
Sbjct: 173 IERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSINRKDLVDYI 232

Query: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327
            THY  PR+V+AA+G V H++ +E  K  F +  S  T   ++ A  P  FTGSE+R+ D
Sbjct: 233 TTHYKGPRIVLAAAGGVSHDELLELAKFHFGE--SLSTHKGEIPALPPCKFTGSEIRVRD 292

Query: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMGSELAQRVAINEVAE 387
           D +PLA  A+A     W  PD+I LMV   ++G+W++S GGG ++ S+LAQ      +  
Sbjct: 293 DKMPLAHLAVAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCH 352

Query: 388 SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYETTKLAYRVSEPDVTRARNQLKSSL 447
           S  +FNT+Y DTGL+G+Y V +P  + D+ + +  E  +L   V+E +V RA+N LK+++
Sbjct: 353 SFQSFNTSYTDTGLWGIYMVCEPATVADMLHVVQKEWMRLCTSVTESEVARAKNLLKTNM 412

Query: 448 LLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANRFIYDRDVAIAAL 507
           LL +DG++P+ EDIGRQ+L Y RRIP  EL ARIDAV+A  I+ V  ++IYD+  A+AA+
Sbjct: 413 LLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVNAEIIREVCTKYIYDKSPAVAAV 472

Query: 508 GPIQGLPDYNWFRRRTYW 526
           GPI+ LPD+N       W
Sbjct: 473 GPIEQLPDFNQICSNMRW 487

BLAST of MS014516 vs. ExPASy Swiss-Prot
Match: Q5REK3 (Mitochondrial-processing peptidase subunit beta OS=Pongo abelii OX=9601 GN=PMPCB PE=2 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.4e-130
Identity = 234/440 (53.18%), Postives = 309/440 (70.23%), Query Frame = 0

Query: 88  ILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETEETNGTAHFLEHMIFKG 147
           +L+ PETRVT L +GLRVA+E +    T TVG+WIDAGSR+E E+ NGTAHFLEHM FKG
Sbjct: 52  VLNVPETRVTCLESGLRVASEDS-GLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKG 111

Query: 148 TEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKALDILADILQNSKFDEHR 207
           T+KR+   LE EIENMG HLNAYTSREQT YYAK   KD+P+A++ILADI+QNS   E  
Sbjct: 112 TKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAE 171

Query: 208 ISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGPAQNIRTITKDHLQSYI 267
           I RER VILREM+EVE   +EV+FD+LHATA+Q T LGRTILGP +NI++I++  L  YI
Sbjct: 172 IERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYI 231

Query: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327
            THY  PR+V+AA+G V H++ ++  K  F    S  T   ++ A  P  FTGSE+R+ D
Sbjct: 232 TTHYKGPRIVLAAAGGVSHDELLDLAKFHFG--DSLCTHKGEIPALPPCKFTGSEIRVRD 291

Query: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMGSELAQRVAINEVAE 387
           D +PLA  AIA     W  PD+I LMV   ++G+W++S GGG ++ S+LAQ      +  
Sbjct: 292 DKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCH 351

Query: 388 SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYETTKLAYRVSEPDVTRARNQLKSSL 447
           S  +FNT+Y DTGL+G+Y V +P  + D+ + +  E  +L   V+E +V RARN LK+++
Sbjct: 352 SFQSFNTSYTDTGLWGLYMVCEPSTVADMLHVVQKEWMRLCTSVTESEVARARNLLKTNM 411

Query: 448 LLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANRFIYDRDVAIAAL 507
           LL +DG++P+ EDIGRQ+L Y RRIP  EL ARIDAV+A TI+ V  ++IY+R  AIAA+
Sbjct: 412 LLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVNAETIREVCTKYIYNRSPAIAAV 471

Query: 508 GPIQGLPDYNWFRRRTYWNR 528
           GPI+ LPD+        W R
Sbjct: 472 GPIEQLPDFKQICSNMCWLR 488

BLAST of MS014516 vs. ExPASy Swiss-Prot
Match: O75439 (Mitochondrial-processing peptidase subunit beta OS=Homo sapiens OX=9606 GN=PMPCB PE=1 SV=2)

HSP 1 Score: 466.8 bits (1200), Expect = 3.1e-130
Identity = 234/440 (53.18%), Postives = 309/440 (70.23%), Query Frame = 0

Query: 88  ILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETEETNGTAHFLEHMIFKG 147
           +L+ PETRVT L +GLRVA+E +    T TVG+WIDAGSR+E E+ NGTAHFLEHM FKG
Sbjct: 52  VLNVPETRVTCLESGLRVASEDS-GLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKG 111

Query: 148 TEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKALDILADILQNSKFDEHR 207
           T+KR+   LE EIENMG HLNAYTSREQT YYAK   KD+P+A++ILADI+QNS   E  
Sbjct: 112 TKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAE 171

Query: 208 ISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGPAQNIRTITKDHLQSYI 267
           I RER VILREM+EVE   +EV+FD+LHATA+Q T LGRTILGP +NI++I++  L  YI
Sbjct: 172 IERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYI 231

Query: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327
            THY  PR+V+AA+G V H++ ++  K  F    S  T   ++ A  P  FTGSE+R+ D
Sbjct: 232 TTHYKGPRIVLAAAGGVSHDELLDLAKFHFG--DSLCTHKGEIPALPPCKFTGSEIRVRD 291

Query: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMGSELAQRVAINEVAE 387
           D +PLA  AIA     W  PD+I LMV   ++G+W++S GGG ++ S+LAQ      +  
Sbjct: 292 DKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCH 351

Query: 388 SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYETTKLAYRVSEPDVTRARNQLKSSL 447
           S  +FNT+Y DTGL+G+Y V +   + D+ + +  E  +L   V+E +V RARN LK+++
Sbjct: 352 SFQSFNTSYTDTGLWGLYMVCESSTVADMLHVVQKEWMRLCTSVTESEVARARNLLKTNM 411

Query: 448 LLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANRFIYDRDVAIAAL 507
           LL +DG++P+ EDIGRQ+L Y RRIP  EL ARIDAV+A TI+ V  ++IY+R  AIAA+
Sbjct: 412 LLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVNAETIREVCTKYIYNRSPAIAAV 471

Query: 508 GPIQGLPDYNWFRRRTYWNR 528
           GPI+ LPD+   R    W R
Sbjct: 472 GPIKQLPDFKQIRSNMCWLR 488

BLAST of MS014516 vs. ExPASy Swiss-Prot
Match: Q03346 (Mitochondrial-processing peptidase subunit beta OS=Rattus norvegicus OX=10116 GN=Pmpcb PE=1 SV=3)

HSP 1 Score: 466.8 bits (1200), Expect = 3.1e-130
Identity = 235/440 (53.41%), Postives = 306/440 (69.55%), Query Frame = 0

Query: 88  ILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETEETNGTAHFLEHMIFKG 147
           +L+ PET+VT L NGLRVA+E N    T TVG+WIDAGSR+E E+ NGTAHFLEHM FKG
Sbjct: 52  VLNVPETQVTCLENGLRVASE-NSGISTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKG 111

Query: 148 TEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKALDILADILQNSKFDEHR 207
           T+KR+   LE EIENMG HLNAYTSREQT YYAK   KD+P+A++ILADI+QNS   E  
Sbjct: 112 TKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAE 171

Query: 208 ISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGPAQNIRTITKDHLQSYI 267
           I RER VILREM+EVE   +EV+FD+LHATA+Q T LGRTILGP +NI++I++  L  YI
Sbjct: 172 IERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYI 231

Query: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327
            THY  PR+V+AA+G V H + +E  K  F    S       + A  P  FTGSE+R+ D
Sbjct: 232 TTHYKGPRIVLAAAGGVCHNELLELAKFHFG--DSLCAHKGDVPALPPCKFTGSEIRVRD 291

Query: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMGSELAQRVAINEVAE 387
           D +PLA  A+A     WT PD+I LMV   ++G+W++S GGG ++ S+LAQ      +  
Sbjct: 292 DKMPLAHLAVAIEAVGWTHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCH 351

Query: 388 SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYETTKLAYRVSEPDVTRARNQLKSSL 447
           S  +FNT+Y DTGL+G+Y V +   + D+ +A+  E  +L   VSE +V RA+N LK+++
Sbjct: 352 SFQSFNTSYTDTGLWGLYMVCEQATVADMLHAVQKEWMRLCTAVSESEVARAKNLLKTNM 411

Query: 448 LLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANRFIYDRDVAIAAL 507
           LL +DG++P+ EDIGRQ+L Y RRIP  EL ARIDAVDA  ++ V  ++IY +  AIAAL
Sbjct: 412 LLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVDAEMVREVCTKYIYGKSPAIAAL 471

Query: 508 GPIQGLPDYNWFRRRTYWNR 528
           GPI+ LPD+N       W R
Sbjct: 472 GPIERLPDFNQICSNMRWTR 488

BLAST of MS014516 vs. ExPASy TrEMBL
Match: A0A6J1CT95 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014126 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.1e-298
Identity = 527/528 (99.81%), Postives = 527/528 (99.81%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS
Sbjct: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. ExPASy TrEMBL
Match: A0A6J1KG96 (probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492954 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 8.2e-291
Identity = 510/528 (96.59%), Postives = 521/528 (98.67%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MA+K+LLTLAR SHRR SASFS A+RSASTSPAVA+SSPPPSPPPPNAMIYDRLAE VKS
Sbjct: 1   MAIKHLLTLARTSHRRFSASFSRAVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEGVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETR+TTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRITTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTE+RTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTERRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVPKALDILADILQNSKF+EHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPKALDILADILQNSKFEEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVK LFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKNLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTA+QLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTATQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYETTKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYETTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVD+STIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDSSTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. ExPASy TrEMBL
Match: A0A6J1ERB4 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436988 PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 1.8e-290
Identity = 512/528 (96.97%), Postives = 519/528 (98.30%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMK LLTLAR S RRSSASFS  +RSASTSPAVA+SSPPPSPPPPNAM+YDRLAEAVKS
Sbjct: 1   MAMKRLLTLARTSPRRSSASFSQDVRSASTSPAVASSSPPPSPPPPNAMVYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WID+GSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDSGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVP ALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPHALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQF+IAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFSIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYE TKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYEMTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. ExPASy TrEMBL
Match: A0A0A0L0I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G093000 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 508/528 (96.21%), Postives = 520/528 (98.48%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MA+++LLTLAR SHRRS A FS A+RSASTSPAVA+SSPPPSPPPPNAMIYDRLAEAVKS
Sbjct: 1   MAIRHLLTLARTSHRRSPALFSQAVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTL NGLRVATESNL ARTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLSNGLRVATESNLTARTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKR+ARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFT+L
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTQL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTA+QLVAKEPAIFTGSEVRI+DDD+PLAQFAIAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTATQLVAKEPAIFTGSEVRIVDDDIPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYETTKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYETTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. ExPASy TrEMBL
Match: A0A6J1IZR5 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479917 PE=3 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.5e-289
Identity = 511/528 (96.78%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRRSSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEAVKS 60
           MAMK LLTLAR S RRSSASFS  +RSASTSPAVA+SSPPPSPPPPNAMI+DRLAEAVKS
Sbjct: 1   MAMKRLLTLARTSPRRSSASFSQDVRSASTSPAVASSSPPPSPPPPNAMIFDRLAEAVKS 60

Query: 61  KLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGV 120
           KLKQ ENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLA+RTATVGV
Sbjct: 61  KLKQLENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLASRTATVGV 120

Query: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180
           WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA
Sbjct: 121 WIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYA 180

Query: 181 KVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240
           KVLDKDVP ALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ
Sbjct: 181 KVLDKDVPNALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQ 240

Query: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300
           YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL
Sbjct: 241 YTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKL 300

Query: 301 SSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQAMLG 360
           S+EPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQ +IAFNGASWTDPDSIALMVMQAMLG
Sbjct: 301 SAEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQLSIAFNGASWTDPDSIALMVMQAMLG 360

Query: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420
           SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI
Sbjct: 361 SWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAI 420

Query: 421 MYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480
           MYE TKLAYRVSE DVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR
Sbjct: 421 MYEMTKLAYRVSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFAR 480

Query: 481 IDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           IDAVDASTIKRVANRFIYDRD+AIAALGPIQGLPDYNWFRRRTYWNRY
Sbjct: 481 IDAVDASTIKRVANRFIYDRDIAIAALGPIQGLPDYNWFRRRTYWNRY 528

BLAST of MS014516 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 840.9 bits (2171), Expect = 5.7e-244
Identity = 420/531 (79.10%), Postives = 477/531 (89.83%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRR---SSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEA 60
           MAMKNLL+LAR S RR   + A+ S +  SA  S   +AS    SPPPP+ M YD  AE 
Sbjct: 1   MAMKNLLSLARRSQRRLFLTQATRSSSSFSAIDSVPASASPTALSPPPPHLMPYDHAAEI 60

Query: 61  VKSKLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTAT 120
           +K+K+K+ ENPD RFLKY SPHP +  H  ILSAPETRVTTLPNGLRVATESNL+A+TAT
Sbjct: 61  IKNKIKKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTAT 120

Query: 121 VGVWIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTT 180
           VGVWIDAGSRFE++ETNGTAHFLEHMIFKGT++RT R LEEEIE++GGHLNAYTSREQTT
Sbjct: 121 VGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTT 180

Query: 181 YYAKVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHAT 240
           YYAKVLD +V +ALD+LADILQNSKF+E RI+RERDVILREM+EVEGQT+EV+ DHLHAT
Sbjct: 181 YYAKVLDSNVNQALDVLADILQNSKFEEQRINRERDVILREMQEVEGQTDEVVLDHLHAT 240

Query: 241 AFQYTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLF 300
           AFQYTPLGRTILGPAQN+++IT++ LQ+YI+THYTA RMVIAA+GAVKHE+ VEQVKKLF
Sbjct: 241 AFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLF 300

Query: 301 TKLSSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQA 360
           TKLSS+PTT SQLVA EPA FTGSEVR+IDDD+PLAQFA+AF GASWTDPDS+ALMVMQ 
Sbjct: 301 TKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQT 360

Query: 361 MLGSWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLA 420
           MLGSWNK+ GGGKH+GS+L QRVAINE+AES+MAFNTNYKDTGLFGVYAVAK DCLDDL+
Sbjct: 361 MLGSWNKNVGGGKHVGSDLTQRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLS 420

Query: 421 YAIMYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAEL 480
           YAIMYE TKLAYRVS+ DVTRARNQLKSSLLLH+DGTSP+AEDIGRQLLTYGRRIP AEL
Sbjct: 421 YAIMYEVTKLAYRVSDADVTRARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAEL 480

Query: 481 FARIDAVDASTIKRVANRFIYDRDVAIAALGPIQGLPDYNWFRRRTYWNRY 529
           FARIDAVDAST+KRVAN++IYD+D+AI+A+GPIQ LPDYN FRRRTYWNRY
Sbjct: 481 FARIDAVDASTVKRVANKYIYDKDIAISAIGPIQDLPDYNKFRRRTYWNRY 531

BLAST of MS014516 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 788.5 bits (2035), Expect = 3.3e-228
Identity = 397/503 (78.93%), Postives = 451/503 (89.66%), Query Frame = 0

Query: 1   MAMKNLLTLARNSHRR---SSASFSHAIRSASTSPAVAASSPPPSPPPPNAMIYDRLAEA 60
           MAMKNLL+LAR S RR   + A+ S +  SA  S   +AS    SPPPP+ M YD  AE 
Sbjct: 1   MAMKNLLSLARRSQRRLFLTQATRSSSSFSAIDSVPASASPTALSPPPPHLMPYDHAAEI 60

Query: 61  VKSKLKQFENPDPRFLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTAT 120
           +K+K+K+ ENPD RFLKY SPHP +  H  ILSAPETRVTTLPNGLRVATESNL+A+TAT
Sbjct: 61  IKNKIKKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTAT 120

Query: 121 VGVWIDAGSRFETEETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTT 180
           VGVWIDAGSRFE++ETNGTAHFLEHMIFKGT++RT R LEEEIE++GGHLNAYTSREQTT
Sbjct: 121 VGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTT 180

Query: 181 YYAKVLDKDVPKALDILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHAT 240
           YYAKVLD +V +ALD+LADILQNSKF+E RI+RERDVILREM+EVEGQT+EV+ DHLHAT
Sbjct: 181 YYAKVLDSNVNQALDVLADILQNSKFEEQRINRERDVILREMQEVEGQTDEVVLDHLHAT 240

Query: 241 AFQYTPLGRTILGPAQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLF 300
           AFQYTPLGRTILGPAQN+++IT++ LQ+YI+THYTA RMVIAA+GAVKHE+ VEQVKKLF
Sbjct: 241 AFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLF 300

Query: 301 TKLSSEPTTASQLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASWTDPDSIALMVMQA 360
           TKLSS+PTT SQLVA EPA FTGSEVR+IDDD+PLAQFA+AF GASWTDPDS+ALMVMQ 
Sbjct: 301 TKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQT 360

Query: 361 MLGSWNKSAGGGKHMGSELAQRVAINEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLA 420
           MLGSWNK+ GGGKH+GS+L QRVAINE+AES+MAFNTNYKDTGLFGVYAVAK DCLDDL+
Sbjct: 361 MLGSWNKNVGGGKHVGSDLTQRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLS 420

Query: 421 YAIMYETTKLAYRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAEL 480
           YAIMYE TKLAYRVS+ DVTRARNQLKSSLLLH+DGTSP+AEDIGRQLLTYGRRIP AEL
Sbjct: 421 YAIMYEVTKLAYRVSDADVTRARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAEL 480

Query: 481 FARIDAVDASTIKRVANRFIYDR 501
           FARIDAVDAST+KRVAN++IYD+
Sbjct: 481 FARIDAVDASTVKRVANKYIYDK 503

BLAST of MS014516 vs. TAIR 10
Match: AT3G16480.1 (mitochondrial processing peptidase alpha subunit )

HSP 1 Score: 197.6 bits (501), Expect = 2.5e-50
Identity = 139/451 (30.82%), Postives = 232/451 (51.44%), Query Frame = 0

Query: 80  PTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETEETNGTAHF 139
           P ++DH   +   + + TTLPNGL +ATE +     A++G+++D GS +ET +  G  H 
Sbjct: 63  PPLSDH---VEPSKLKTTTLPNGLTIATEMS-PNPAASIGLYVDCGSIYETPQFRGATHL 122

Query: 140 LEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKALDILADILQ 199
           LE M FK T  R+  +L  EIE +GG+ +A  SREQ  Y    L   VP+ +++L D ++
Sbjct: 123 LERMAFKSTLNRSHFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVLIDSVR 182

Query: 200 NSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGPAQNIRTIT 259
           N  F +  ++ E   +  E+ E        + + +H+  +    L   +  P   I  +T
Sbjct: 183 NPAFLDWEVNEELRKVKVEIGEFATNPMGFLLEAVHSAGYS-GALANPLYAPESAITGLT 242

Query: 260 KDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTA---SQLVAKEPA 319
            + L++++  +YTA RMV+AASG V HE+ ++ V+ L + L + P  A   SQ V  +  
Sbjct: 243 GEVLENFVFENYTASRMVLAASG-VDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFR 302

Query: 320 IFTGSEVRIIDDDVPLAQFAIAFNGASW-TDPDSIALMVMQAMLGSWNK-SAGG-GKHMG 379
             TG E            FA+AF    W  + ++I   V+Q ++G     SAGG GK M 
Sbjct: 303 QHTGGEA---------THFALAFEVPGWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMH 362

Query: 380 SELAQRVA-INEVAESMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYETTKLA-YRV 439
           S L  R+   ++  +S  AF + + +TGLFG+Y    P+        +  E   +A  +V
Sbjct: 363 SWLYLRLLNQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVADGKV 422

Query: 440 SEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKR 499
           ++  + RA+   KS++L++++     AEDIGRQ+LTYG R P  +    +D +    I  
Sbjct: 423 NQKHLDRAKAATKSAILMNLESRMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIAD 482

Query: 500 VANRFIYDRDVAIAALGPIQGLPDYNWFRRR 523
             ++ I  + + +A  G +  +P Y+   +R
Sbjct: 483 FTSKVI-TKPLTMATFGDVLNVPSYDSVSKR 497

BLAST of MS014516 vs. TAIR 10
Match: AT1G51980.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-47
Identity = 153/517 (29.59%), Postives = 263/517 (50.87%), Query Frame = 0

Query: 16  RSSASFSHAIR---SASTSPAVAASSPPPSPPPPNAMIY-DRLAEAVKSKLKQFENPDPR 75
           R++AS + A++   + S  PA  ASS   +    +   Y   L+   ++ L   + P   
Sbjct: 3   RTAASRARALKGVLTRSLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMP--- 62

Query: 76  FLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETE 135
            L+  S  P + D    +   + ++TTLPNGL++A+E+      A++G+++D GS +E  
Sbjct: 63  -LQGVSLPPPLADK---VEPSKLQITTLPNGLKIASETT-PNPAASIGLYVDCGSIYEAP 122

Query: 136 ETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKAL 195
             +G  H LE M FK T  RT  +L  EIE +GG+ +A  SREQ +Y    L   VP+ +
Sbjct: 123 YFHGATHLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMV 182

Query: 196 DILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGP 255
           ++L D ++N  F +  ++ E   +  E+ E+       + + +H+  +   PL   +  P
Sbjct: 183 EVLIDSVRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYS-GPLASPLYAP 242

Query: 256 AQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEP---TTAS 315
              +  +  + L+ ++  ++TA RMV+AASG V+HE+ ++  + L + L + P      S
Sbjct: 243 ESALDRLNGELLEEFMTENFTAARMVLAASG-VEHEELLKVAEPLTSDLPNVPPQLAPKS 302

Query: 316 QLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASW-TDPDSIALMVMQAMLGSWNK-SA 375
           Q V  +    TG E            FA+AF    W  + +++   V+Q ++G     SA
Sbjct: 303 QYVGGDFRQHTGGEA---------THFAVAFEVPGWNNEKEAVTATVLQMLMGGGGSFSA 362

Query: 376 GG-GKHMGSELAQRVAINEVAE--SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYE 435
           GG GK M S L +RV +NE  E  S  AF + + DTGLFG+Y  + P            E
Sbjct: 363 GGPGKGMHSWLYRRV-LNEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKE 422

Query: 436 TTKLA-YRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARID 495
              +A  +V++  + RA+   KS++L++++     AEDIGRQ+LTYG R P  +    + 
Sbjct: 423 LKDVAGGKVNQAHLDRAKAATKSAVLMNLESRMIAAEDIGRQILTYGERKPVDQF---LK 482

Query: 496 AVDASTIKRVAN--RFIYDRDVAIAALGPIQGLPDYN 518
           +VD  T+K +A+    +  + + + + G +  +P Y+
Sbjct: 483 SVDQLTLKDIADFTSKVISKPLTMGSFGDVLAVPSYD 496

BLAST of MS014516 vs. TAIR 10
Match: AT1G51980.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 172.9 bits (437), Expect = 6.7e-43
Identity = 143/467 (30.62%), Postives = 239/467 (51.18%), Query Frame = 0

Query: 16  RSSASFSHAIR---SASTSPAVAASSPPPSPPPPNAMIY-DRLAEAVKSKLKQFENPDPR 75
           R++AS + A++   + S  PA  ASS   +    +   Y   L+   ++ L   + P   
Sbjct: 3   RTAASRARALKGVLTRSLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMP--- 62

Query: 76  FLKYGSPHPTITDHTRILSAPETRVTTLPNGLRVATESNLAARTATVGVWIDAGSRFETE 135
            L+  S  P + D    +   + ++TTLPNGL++A+E+      A++G+++D GS +E  
Sbjct: 63  -LQGVSLPPPLADK---VEPSKLQITTLPNGLKIASETT-PNPAASIGLYVDCGSIYEAP 122

Query: 136 ETNGTAHFLEHMIFKGTEKRTARQLEEEIENMGGHLNAYTSREQTTYYAKVLDKDVPKAL 195
             +G  H LE M FK T  RT  +L  EIE +GG+ +A  SREQ +Y    L   VP+ +
Sbjct: 123 YFHGATHLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMV 182

Query: 196 DILADILQNSKFDEHRISRERDVILREMEEVEGQTEEVIFDHLHATAFQYTPLGRTILGP 255
           ++L D ++N  F +  ++ E   +  E+ E+       + + +H+  +   PL   +  P
Sbjct: 183 EVLIDSVRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYS-GPLASPLYAP 242

Query: 256 AQNIRTITKDHLQSYIQTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEP---TTAS 315
              +  +  + L+ ++  ++TA RMV+AASG V+HE+ ++  + L + L + P      S
Sbjct: 243 ESALDRLNGELLEEFMTENFTAARMVLAASG-VEHEELLKVAEPLTSDLPNVPPQLAPKS 302

Query: 316 QLVAKEPAIFTGSEVRIIDDDVPLAQFAIAFNGASW-TDPDSIALMVMQAMLGSWNK-SA 375
           Q V  +    TG E            FA+AF    W  + +++   V+Q ++G     SA
Sbjct: 303 QYVGGDFRQHTGGEA---------THFAVAFEVPGWNNEKEAVTATVLQMLMGGGGSFSA 362

Query: 376 GG-GKHMGSELAQRVAINEVAE--SMMAFNTNYKDTGLFGVYAVAKPDCLDDLAYAIMYE 435
           GG GK M S L +RV +NE  E  S  AF + + DTGLFG+Y  + P            E
Sbjct: 363 GGPGKGMHSWLYRRV-LNEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKE 422

Query: 436 TTKLA-YRVSEPDVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYG 470
              +A  +V++  + RA+   KS++L++++     AEDIGRQ+LTYG
Sbjct: 423 LKDVAGGKVNQAHLDRAKAATKSAVLMNLESRMIAAEDIGRQILTYG 449