Homology
BLAST of MS014512 vs. NCBI nr
Match:
XP_022144477.1 (alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic [Momordica charantia])
HSP 1 Score: 1908.3 bits (4942), Expect = 0.0e+00
Identity = 974/1037 (93.92%), Postives = 974/1037 (93.92%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS
Sbjct: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY
Sbjct: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE
Sbjct: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDLSAFNAGEHTKASEALASADK
Sbjct: 301 NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA
Sbjct: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ
Sbjct: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV 660
AKSIGEDETVEEEDEPESKGIQ KKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV
Sbjct: 601 AKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV 660
Query: 661 KDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 720
KDEVFNAFYKLWP KFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK
Sbjct: 661 KDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 720
Query: 721 MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNIL 780
MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLLNIL
Sbjct: 721 MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLLNIL 780
Query: 781 GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 840
GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG
Sbjct: 781 GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 840
Query: 841 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 900
DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV
Sbjct: 841 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 900
Query: 901 EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI 960
EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI
Sbjct: 901 EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI 960
Query: 961 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT 1020
GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT
Sbjct: 961 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT 977
Query: 1021 IHEYAKDIWNIKPVELP 1038
IHEYAKDIWNIKPVELP
Sbjct: 1021 IHEYAKDIWNIKPVELP 977
BLAST of MS014512 vs. NCBI nr
Match:
XP_038895156.1 (alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Benincasa hispida])
HSP 1 Score: 1728.4 bits (4475), Expect = 0.0e+00
Identity = 885/1037 (85.34%), Postives = 919/1037 (88.62%), Query Frame = 0
Query: 3 ASSQLTVNLNRADSFSHANSF-PTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSSE 62
A+SQ TV LNRADSFSH+NSF P L+GLS +Y +SKLLLV TS WRS +RTF VKNVSSE
Sbjct: 2 ATSQFTVALNRADSFSHSNSFPPPLIGLSSRYRQSKLLLVPTSTWRSPKRTFLVKNVSSE 61
Query: 63 PKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS 122
PK K DPVADEES I ASAF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS
Sbjct: 62 PKLK---DPVADEESPIAASAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS 121
Query: 123 VRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYE 182
VRDALIINWN TYELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG+YAEAL+KLGYE
Sbjct: 122 VRDALIINWNETYELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGSYAEALNKLGYE 181
Query: 183 LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEV 242
LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEEV
Sbjct: 182 LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEEV 241
Query: 243 AENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN 302
AENWLEIGNPWE+ RNDIKY IKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN
Sbjct: 242 AENWLEIGNPWEIFRNDIKYHIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN 301
Query: 303 TINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIG 362
TINLRLWSTKAP EDFDLSAFNAGEHT+ASEALA+A+K
Sbjct: 302 TINLRLWSTKAPTEDFDLSAFNAGEHTRASEALANAEK---------------------- 361
Query: 363 LKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQY 422
ICHVLYPGDDSIEGKILRLKQQY
Sbjct: 362 -------------------------------------ICHVLYPGDDSIEGKILRLKQQY 421
Query: 423 TLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWE 482
TLCSASLQDIVA FERRSG NKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWE
Sbjct: 422 TLCSASLQDIVACFERRSGTNKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWE 481
Query: 483 EAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTAD 542
EAW ITQRT+AYTNHTVLPEALEKWS ELMQ+LLPRHVEIIE IDEELI+TIISEYG AD
Sbjct: 482 EAWNITQRTMAYTNHTVLPEALEKWSFELMQKLLPRHVEIIELIDEELIRTIISEYGKAD 541
Query: 543 LKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQA 602
LKLL +KLKELRILENVDLPAAYSDL IEPEESS + STE + S E DS D+EN A
Sbjct: 542 LKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIVSTEVLKSSEEADSVDKENLSKLA 601
Query: 603 KSIGEDETVEEEDEPESKGIQAKKVEPI-PPPPKMVRMANLCVAGGHAVNGVAEIHSEIV 662
KS+ + E VE D+PESK IQ K VEPI PPPPKMVRMANLCV GGHAVNGVAEIHSEIV
Sbjct: 602 KSVDKVEFVEANDDPESKDIQDKNVEPIPPPPPKMVRMANLCVVGGHAVNGVAEIHSEIV 661
Query: 663 KDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 722
KDEVFNAFY+LWP KFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK
Sbjct: 662 KDEVFNAFYELWPEKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 721
Query: 723 MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNIL 782
FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLLNI
Sbjct: 722 EFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLLNIF 781
Query: 783 GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 842
GIVYRYKKMKEMSA+ERKE +VPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG
Sbjct: 782 GIVYRYKKMKEMSAKERKETYVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 841
Query: 843 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 902
DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV
Sbjct: 842 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 901
Query: 903 EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI 962
EIRQEVGA+NFFLFGA+AHEIAGLRKERA GKFIPDPRFEEVKEYVRSGVFGSYDYE++I
Sbjct: 902 EIRQEVGADNFFLFGAEAHEIAGLRKERAVGKFIPDPRFEEVKEYVRSGVFGSYDYEDLI 961
Query: 963 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT 1022
GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQK+WTRMSILNTAGSYKFSSDRT
Sbjct: 962 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKRWTRMSILNTAGSYKFSSDRT 975
Query: 1023 IHEYAKDIWNIKPVELP 1038
IHEYAKDIW+IKPVELP
Sbjct: 1022 IHEYAKDIWSIKPVELP 975
BLAST of MS014512 vs. NCBI nr
Match:
TYK15871.1 (alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa])
HSP 1 Score: 1703.7 bits (4411), Expect = 0.0e+00
Identity = 873/1040 (83.94%), Postives = 914/1040 (87.88%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KLKELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. NCBI nr
Match:
XP_008455590.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic-like [Cucumis melo])
HSP 1 Score: 1701.4 bits (4405), Expect = 0.0e+00
Identity = 872/1040 (83.85%), Postives = 913/1040 (87.79%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KLKELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKE TGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEXTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. NCBI nr
Match:
KAA0053393.1 (alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa])
HSP 1 Score: 1700.3 bits (4402), Expect = 0.0e+00
Identity = 871/1040 (83.75%), Postives = 913/1040 (87.79%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KL ELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLNELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQ++VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQKKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. ExPASy Swiss-Prot
Match:
P53536 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia faba OX=3906 GN=PHO1 PE=2 SV=2)
HSP 1 Score: 1484.9 bits (3843), Expect = 0.0e+00
Identity = 751/1032 (72.77%), Postives = 856/1032 (82.95%), Query Frame = 0
Query: 35 KSKLLLVRTS--NWRSARR--------TFAVK-NVSSEPKQKLKDDPVADEESSIVASAF 94
+S L VRT+ +RS +R F+VK +E KQK+KD V +E+ S+F
Sbjct: 32 RSSSLFVRTNVIKYRSVKRNLEFRRRSAFSVKCGSGNEAKQKVKDQEV-QQEAKTSPSSF 91
Query: 95 TPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWNATYELYEKLNVK 154
PD SI SSIKYHAEFTPLFSP++F+LP+AF ATAQSVRDALIINWNATY+ YEKLNVK
Sbjct: 92 APDTTSIVSSIKYHAEFTPLFSPEKFELPQAFIATAQSVRDALIINWNATYDYYEKLNVK 151
Query: 155 QAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPDAALGNGGLGRLA 214
QAYYLSMEFLQGRALLNAIGNLELTG YAEALS+L Y+LE+VA QEPDAALGNGGLGRLA
Sbjct: 152 QAYYLSMEFLQGRALLNAIGNLELTGPYAEALSQLSYKLEDVAHQEPDAALGNGGLGRLA 211
Query: 215 SCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNPWEVVRNDIKYPI 274
SCFLDSLATLNYPAWGYGLRY+YGLFKQ+ITKDGQEEVAE+WLE+GNPWE+VRND+ YP+
Sbjct: 212 SCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEMGNPWEIVRNDVSYPV 271
Query: 275 KFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTKAPAEDFDLSAFN 334
+FYGKVV GSDGKK+W GGEDI+AVA+DVPIPGYKT++TINLRLWSTKA +E+FDL+AFN
Sbjct: 272 RFYGKVVSGSDGKKHWVGGEDIKAVAHDVPIPGYKTRSTINLRLWSTKAASEEFDLNAFN 331
Query: 335 AGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLVRKIENFMKRI 394
+G HT+ASEALA+A+K
Sbjct: 332 SGRHTEASEALANAEK-------------------------------------------- 391
Query: 395 CTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANK 454
IC++LYPGD+SIEGK LRLKQQYTLCSASLQDI+ARFERRSGA+
Sbjct: 392 ---------------ICYILYPGDESIEGKTLRLKQQYTLCSASLQDIIARFERRSGASV 451
Query: 455 KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEAL 514
WE+FPEKVAVQMNDTHPTLCIPELMRIL+D+KGLSW++AW ITQRTVAYTNHTVLPEAL
Sbjct: 452 NWEDFPEKVAVQMNDTHPTLCIPELMRILIDIKGLSWKDAWNITQRTVAYTNHTVLPEAL 511
Query: 515 EKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAA 574
EKWS++LM++LLPRHVEIIE IDEELI+TII+EYGTAD LL KKLKE+RILENV+LPA
Sbjct: 512 EKWSMDLMEKLLPRHVEIIEMIDEELIRTIIAEYGTADSDLLDKKLKEMRILENVELPAE 571
Query: 575 YSDLIIEPEESSVVAST---------EEPEISNEVDSADEENSEGQAKSIGED------- 634
++D++++ +E++ ++S EE E S E +EE G + G+D
Sbjct: 572 FADILVKTKEATDISSEEVQISKEGGEEEETSKEGGEEEEEKEVGGGREEGDDGKEDEVE 631
Query: 635 ETVEEEDEPESKGIQAKK---VEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEV 694
+ + E+D I KK EP+P PPK+VRMANLCV GGHAVNGVAEIHSEIVKD+V
Sbjct: 632 KAIAEKDGTVKSSIGDKKKKLPEPVPVPPKLVRMANLCVVGGHAVNGVAEIHSEIVKDDV 691
Query: 695 FNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFAD 754
FNAFYKLWP KFQNKTNGVTPRRWIRFCNPDLSK+IT WIG+EDW+LNTEKL EL+ FAD
Sbjct: 692 FNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKIITQWIGTEDWILNTEKLAELRKFAD 751
Query: 755 NEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVY 814
NEDLQTQWR AKRNNK+K +FL+E+TGY+VSPD+MFDIQ VKRIHEYKRQLLNI GIVY
Sbjct: 752 NEDLQTQWREAKRNNKVKVAAFLRERTGYSVSPDSMFDIQ-VKRIHEYKRQLLNIFGIVY 811
Query: 815 RYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLK 874
RYKKMKEM+A ERKE FVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLK
Sbjct: 812 RYKKMKEMNAAERKENFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLK 871
Query: 875 VIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQ 934
VIFVPDYNVSVAE+LIPASELSQHISTAGMEASGTSNMKFAMNGC+ IGTLDGANVEIR+
Sbjct: 872 VIFVPDYNVSVAEMLIPASELSQHISTAGMEASGTSNMKFAMNGCLQIGTLDGANVEIRE 931
Query: 935 EVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLE 994
EVGA+NFFLFGAKA EI GLRKERA GKF+PDPRFEEVK++VRSGVFGSY+Y+E+IGSLE
Sbjct: 932 EVGADNFFLFGAKAREIVGLRKERARGKFVPDPRFEEVKKFVRSGVFGSYNYDELIGSLE 991
Query: 995 GNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEY 1037
GNEGFGRADYFLVG+DFPSY+ECQE+VD+AYRDQKKWTRMSILNTAGS KFSSDRTIHEY
Sbjct: 992 GNEGFGRADYFLVGQDFPSYLECQEEVDKAYRDQKKWTRMSILNTAGSSKFSSDRTIHEY 1002
BLAST of MS014512 vs. ExPASy Swiss-Prot
Match:
P04045 (Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 PE=1 SV=2)
HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 738/1030 (71.65%), Postives = 836/1030 (81.17%), Query Frame = 0
Query: 12 NRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSSEPKQKLKDDPV 71
N A F+H +S + + + SKL L +TS++R +R F V N SE P+
Sbjct: 5 NGAHLFNHYSSNSRFIHFTSRNTSSKLFLTKTSHFRRPKRCFHVNNTLSEKIH----HPI 64
Query: 72 ADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWN 131
++ S+F PDAASI SSIKYHAEFTP+FSP+RF+LPKAFFATAQSVRD+L+INWN
Sbjct: 65 TEQGGESDLSSFAPDAASITSSIKYHAEFTPVFSPERFELPKAFFATAQSVRDSLLINWN 124
Query: 132 ATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPD 191
ATY++YEKLN+KQAYYLSMEFLQGRALLNAIGNLELTGA+AEAL LG+ LENVA QEPD
Sbjct: 125 ATYDIYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGAFAEALKNLGHNLENVASQEPD 184
Query: 192 AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNP 251
AALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQ+ITKDGQEEVAE+WLEIG+P
Sbjct: 185 AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEIGSP 244
Query: 252 WEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTK 311
WEVVRND+ YPIKFYGKV GSDGK+ W GGEDI+AVAYDVPIPGYKT+ TI+LRLWST+
Sbjct: 245 WEVVRNDVSYPIKFYGKVSTGSDGKRYWIGGEDIKAVAYDVPIPGYKTRTTISLRLWSTQ 304
Query: 312 APAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEF 371
P+ DFDLSAFNAGEHTKA EA A+A+K
Sbjct: 305 VPSADFDLSAFNAGEHTKACEAQANAEK-------------------------------- 364
Query: 372 LVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDI 431
IC++LYPGD+S EGKILRLKQQYTLCSASLQDI
Sbjct: 365 ---------------------------ICYILYPGDESEEGKILRLKQQYTLCSASLQDI 424
Query: 432 VARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTV 491
++RFERRSG KWEEFPEKVAVQMNDTHPTLCIPELMRIL+DLKGL+W EAW ITQRTV
Sbjct: 425 ISRFERRSGDRIKWEEFPEKVAVQMNDTHPTLCIPELMRILIDLKGLNWNEAWNITQRTV 484
Query: 492 AYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKE 551
AYTNHTVLPEALEKWS ELMQ+LLPRHVEIIE IDEEL+ I+ +YG+ DL L +KL
Sbjct: 485 AYTNHTVLPEALEKWSYELMQKLLPRHVEIIEAIDEELVHEIVLKYGSMDLNKLEEKLTT 544
Query: 552 LRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSAD-----EENSEGQAKSIGE 611
+RILEN DLP++ ++L I+P E SV TE E+ ++V+++D +E+ G+ S+
Sbjct: 545 MRILENFDLPSSVAELFIKP-EISVDDDTETVEVHDKVEASDKVVTNDEDDTGKKTSVKI 604
Query: 612 DETVEEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFN 671
+ E++ + ++ EP PPK VRMANLCV GGHAVNGVAEIHSEIVK+EVFN
Sbjct: 605 EAAAEKDIDKKT----PVSPEPAVIPPKKVRMANLCVVGGHAVNGVAEIHSEIVKEEVFN 664
Query: 672 AFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNE 731
FY+LWP KFQNKTNGVTPRRWIRFCNP LS +IT W G+EDWVL TEKL EL+ FADNE
Sbjct: 665 DFYELWPEKFQNKTNGVTPRRWIRFCNPPLSAIITKWTGTEDWVLKTEKLAELQKFADNE 724
Query: 732 DLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVYRY 791
DLQ +WR AKR+NK+K VSFLKEKTGY+V PDAMFDIQ VKRIHEYKRQLLNI GIVYRY
Sbjct: 725 DLQNEWREAKRSNKIKVVSFLKEKTGYSVVPDAMFDIQ-VKRIHEYKRQLLNIFGIVYRY 784
Query: 792 KKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVI 851
KKMKEM+A ERK FVPRVCIFGGKAFATYVQAKRIVKFITDVGAT+NHDPEIGDLLKV+
Sbjct: 785 KKMKEMTAAERKTNFVPRVCIFGGKAFATYVQAKRIVKFITDVGATINHDPEIGDLLKVV 844
Query: 852 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEV 911
FVPDYNVSVAELLIPAS+LS+HISTAGMEASGTSNMKFAMNGCI IGTLDGANVEIR+EV
Sbjct: 845 FVPDYNVSVAELLIPASDLSEHISTAGMEASGTSNMKFAMNGCIQIGTLDGANVEIREEV 904
Query: 912 GAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGN 971
G ENFFLFGA+AHEIAGLRKERA+GKF+PD RFEEVKE+VRSG FGSY+Y+++IGSLEGN
Sbjct: 905 GEENFFLFGAQAHEIAGLRKERADGKFVPDERFEEVKEFVRSGAFGSYNYDDLIGSLEGN 964
Query: 972 EGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAK 1031
EGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WT MSILNTAGSYKFSSDRTIHEYAK
Sbjct: 965 EGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTTMSILNTAGSYKFSSDRTIHEYAK 965
Query: 1032 DIWNIKPVEL 1037
DIWNI+ VE+
Sbjct: 1025 DIWNIEAVEI 965
BLAST of MS014512 vs. ExPASy Swiss-Prot
Match:
P27598 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea batatas OX=4120 PE=2 SV=1)
HSP 1 Score: 1452.6 bits (3759), Expect = 0.0e+00
Identity = 730/999 (73.07%), Postives = 825/999 (82.58%), Query Frame = 0
Query: 42 RTSNWRSARRTFAVKNVSSEPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFT 101
RT+ + +RT VK V E KQ ++ E +++ DAASIASSIKYHAEF+
Sbjct: 30 RTAGLQRTKRTLLVKCVLDETKQTIQHVVTEKNEGTLL------DAASIASSIKYHAEFS 89
Query: 102 PLFSPDRFDLPKAFFATAQSVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNA 161
P FSP+RF+LPKA+FATAQSVRDALI+NWNATY+ YEKLN+KQAYYLSMEFLQGRALLNA
Sbjct: 90 PAFSPERFELPKAYFATAQSVRDALIVNWNATYDYYEKLNMKQAYYLSMEFLQGRALLNA 149
Query: 162 IGNLELTGAYAEALSKLGYELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYG 221
IGNLELTG YAEAL+KLG+ LENVA +EPDAALGNGGLGRLASCFLDSLATLNYPAWGYG
Sbjct: 150 IGNLELTGEYAEALNKLGHNLENVASKEPDAALGNGGLGRLASCFLDSLATLNYPAWGYG 209
Query: 222 LRYRYGLFKQKITKDGQEEVAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTG 281
LRY+YGLFKQ+ITKDGQEEVAE+WLE+GNPWE++R D+ YP+KF+GKV+ GSDGKK+W G
Sbjct: 210 LRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIIRMDVSYPVKFFGKVITGSDGKKHWIG 269
Query: 282 GEDIEAVAYDVPIPGYKTKNTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQ 341
GEDI AVAYDVPIPGYKT+ TI+LRLWSTK P+EDFDL +FNAGEHTKA EA A+A+K
Sbjct: 270 GEDILAVAYDVPIPGYKTRTTISLRLWSTKVPSEDFDLYSFNAGEHTKACEAQANAEK-- 329
Query: 342 LLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICH 401
IC+
Sbjct: 330 ---------------------------------------------------------ICY 389
Query: 402 VLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHP 461
+LYPGD+SIEGKILRLKQQYTLCSASLQDI+ARFERRSG KWEEFPEKVAVQMNDTHP
Sbjct: 390 ILYPGDESIEGKILRLKQQYTLCSASLQDIIARFERRSGEYVKWEEFPEKVAVQMNDTHP 449
Query: 462 TLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEI 521
TLCIPEL+RIL+DLKGLSW+EAW ITQRTVAYTNHTVLPEALEKWS ELM++LLPRH+EI
Sbjct: 450 TLCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSYELMEKLLPRHIEI 509
Query: 522 IEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTE 581
IE IDE+LI I+SEYGT+DL +L KKL ++RILEN D+P++ ++L +P+E+S+V +E
Sbjct: 510 IEMIDEQLINEIVSEYGTSDLDMLEKKLNDMRILENFDIPSSIANLFTKPKETSIVDPSE 569
Query: 582 EPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQAKKVE---PIPPPPKMVRM 641
E E+S +V + E S+ + T E+DE E K + +K E P P PPKMVRM
Sbjct: 570 EVEVSGKVVTESVEVSD-------KVVTESEKDELEEKDTELEKDEDPVPAPIPPKMVRM 629
Query: 642 ANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSK 701
ANLCV GGHAVNGVAEIHS+IVK++VFN FY+LWP KFQNKTNGVTPRRWIRFCNP LS
Sbjct: 630 ANLCVVGGHAVNGVAEIHSDIVKEDVFNDFYQLWPEKFQNKTNGVTPRRWIRFCNPALSN 689
Query: 702 LITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPD 761
+IT WIG+EDWVLNTEKL EL+ FADNEDLQ +WR AKR+NK+K SFLKE+TGY+VSP+
Sbjct: 690 IITKWIGTEDWVLNTEKLAELRKFADNEDLQIEWRAAKRSNKVKVASFLKERTGYSVSPN 749
Query: 762 AMFDIQVVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQ 821
AMFDIQ VKRIHEYKRQLLNILGIVYRYK+MKEMSA ER+ FVPRVCIFGGKAFATYVQ
Sbjct: 750 AMFDIQ-VKRIHEYKRQLLNILGIVYRYKQMKEMSAREREAKFVPRVCIFGGKAFATYVQ 809
Query: 822 AKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASG 881
AKRI KFITDVGAT+NHDPEIGDLLKVIFVPDYNVS AELLIPAS LSQHISTAGMEASG
Sbjct: 810 AKRIAKFITDVGATINHDPEIGDLLKVIFVPDYNVSAAELLIPASGLSQHISTAGMEASG 869
Query: 882 TSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPR 941
SNMKFAMNGCILIGTLDGANVEIRQEVG ENFFLFGA+AHEIAGLRKERAEGKF+PD R
Sbjct: 870 QSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGAEAHEIAGLRKERAEGKFVPDER 929
Query: 942 FEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQ 1001
FEEVKE+++ GVFGS Y+E++GSLEGNEGFGR DYFLVGKDFPSYIECQE+VDEAYRDQ
Sbjct: 930 FEEVKEFIKRGVFGSNTYDELLGSLEGNEGFGRGDYFLVGKDFPSYIECQEKVDEAYRDQ 955
Query: 1002 KKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1038
K WTRMSILNTAGSYKFSSDRTIHEYAKDIWNI+PV P
Sbjct: 990 KIWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIQPVVFP 955
BLAST of MS014512 vs. ExPASy Swiss-Prot
Match:
Q9LIB2 (Alpha-glucan phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=PHS1 PE=1 SV=1)
HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 741/1031 (71.87%), Postives = 837/1031 (81.18%), Query Frame = 0
Query: 14 ADSFSHANSFPTLLGLSHKYGK--SKLLLVRTSNWRSA--RRTF-AVKNVSSEPKQKLKD 73
A+ NS +L+ GK +++ R + R + RR+F +VK++SSEPK K+ D
Sbjct: 13 AEVLIQCNSLSSLVSRRCDDGKWRTRMFPARNRDLRPSPTRRSFLSVKSISSEPKAKVTD 72
Query: 74 DPVADEESSIVAS--AFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDAL 133
+ E+ ++S F PDAAS+ASSIKYHAEFTPLFSP++F+LPKAFFATAQSVRDAL
Sbjct: 73 AVLDSEQEVFISSMNPFAPDAASVASSIKYHAEFTPLFSPEKFELPKAFFATAQSVRDAL 132
Query: 134 IINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVA 193
I+NWNATYE Y ++NVKQAYYLSMEFLQGRAL NA+GNL L AY +AL +LG++LE+VA
Sbjct: 133 IMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGLNSAYGDALKRLGFDLESVA 192
Query: 194 CQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWL 253
QEPD ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ+ITKDGQEE AE+WL
Sbjct: 193 SQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWL 252
Query: 254 EIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLR 313
E+ NPWE+VRND+ YPIKFYGKVV GSDGKK W GGEDI AVAYDVPIPGYKTK TINLR
Sbjct: 253 ELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLR 312
Query: 314 LWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLK 373
LWSTKAP+EDFDLS++N+G+HT+A+EAL +A+K
Sbjct: 313 LWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEK--------------------------- 372
Query: 374 LIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSA 433
IC VLYPGD+S EGK LRLKQQYTLCSA
Sbjct: 373 --------------------------------ICFVLYPGDESTEGKALRLKQQYTLCSA 432
Query: 434 SLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKI 493
SLQDIVARFE RSG N WEEFPEKVAVQMNDTHPTLCIPELMRIL+DLKGLSWE+AWKI
Sbjct: 433 SLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWKI 492
Query: 494 TQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLH 553
TQRTVAYTNHTVLPEALEKWSLELM++LLPRHVEIIE+IDEEL++TI+SEYGTAD LL
Sbjct: 493 TQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKIDEELVRTIVSEYGTADPDLLE 552
Query: 554 KKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQAKSIGE 613
+KLK +RILENV+LP+A++D+I++P V A + N V + EE K+ GE
Sbjct: 553 EKLKAMRILENVELPSAFADVIVKPVNKPVTAK----DAQNGVKTEQEEE-----KTAGE 612
Query: 614 DETVEEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFN 673
EEEDE EP PPKMVRMANL V GGHAVNGVAEIHSEIVK +VFN
Sbjct: 613 ----EEEDEVIP--------EPTVEPPKMVRMANLAVVGGHAVNGVAEIHSEIVKQDVFN 672
Query: 674 AFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNE 733
F +LWP KFQNKTNGVTPRRWIRFCNP LS +ITNWIG+EDWVLNTEK+ EL+ FADNE
Sbjct: 673 DFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAELRKFADNE 732
Query: 734 DLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVYRY 793
DLQ++WR AK+ NKLK VS +KE+TGYTVSPDAMFDIQ +KRIHEYKRQLLNILGIVYRY
Sbjct: 733 DLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFDIQ-IKRIHEYKRQLLNILGIVYRY 792
Query: 794 KKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVI 853
KKMKEMSA ER++AFVPRVCIFGGKAFATYVQAKRIVKFITDV +T+NHDPEIGDLLKVI
Sbjct: 793 KKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEIGDLLKVI 852
Query: 854 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEV 913
FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGANVEIR+EV
Sbjct: 853 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGANVEIREEV 912
Query: 914 GAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGN 973
G ENFFLFGAKA +I LRKERAEGKF+PDP FEEVK++V SGVFGS Y+E+IGSLEGN
Sbjct: 913 GEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSNSYDELIGSLEGN 962
Query: 974 EGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAK 1033
EGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSI+NTAGS+KFSSDRTIHEYAK
Sbjct: 973 EGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAK 962
Query: 1034 DIWNIKPVELP 1038
DIWNIK VELP
Sbjct: 1033 DIWNIKQVELP 962
BLAST of MS014512 vs. ExPASy Swiss-Prot
Match:
P53535 (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=STP-1 PE=1 SV=1)
HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 713/1031 (69.16%), Postives = 832/1031 (80.70%), Query Frame = 0
Query: 18 SHANSFPTLLGLSHKY--GKSKLLLVRTS----NWRSARRTFAVKNVSSEPKQKLKDDPV 77
S NS ++ ++ + S +LL R ++R RR+F+V +V+S+ KQK KD
Sbjct: 7 SGLNSISSISSFNNNFRSKNSNILLSRRRILLFSFRRRRRSFSVSSVASDQKQKTKD--- 66
Query: 78 ADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWN 137
+ + F PD+ S+ SSIKYHAEFTP FSP++F+LPKA++ATA+SVRD LIINWN
Sbjct: 67 SSSDEGFTLDVFQPDSTSVLSSIKYHAEFTPSFSPEKFELPKAYYATAESVRDTLIINWN 126
Query: 138 ATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPD 197
ATYE YEK+NVKQAYYLSMEFLQGRALLNAIGNL LTG YA+AL+KLGY LE+VA QEPD
Sbjct: 127 ATYEFYEKMNVKQAYYLSMEFLQGRALLNAIGNLGLTGPYADALTKLGYSLEDVARQEPD 186
Query: 198 AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNP 257
AALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ ITKDGQEEVAENWLE+GNP
Sbjct: 187 AALGNGGLGRLASCFLDSMATLNYPAWGYGLRYQYGLFKQLITKDGQEEVAENWLEMGNP 246
Query: 258 WEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTK 317
WE+VRNDI YP+KFYGKV+ G+DG+K W GGEDI AVAYDVPIPGYKTK TINLRLW+TK
Sbjct: 247 WEIVRNDISYPVKFYGKVIEGADGRKEWAGGEDITAVAYDVPIPGYKTKTTINLRLWTTK 306
Query: 318 APAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEF 377
AE FDL AFN G+H KA EA A+K
Sbjct: 307 LAAEAFDLYAFNNGDHAKAYEAQKKAEK-------------------------------- 366
Query: 378 LVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDI 437
IC+VLYPGD+S+EGK LRLKQQYTLCSASLQDI
Sbjct: 367 ---------------------------ICYVLYPGDESLEGKTLRLKQQYTLCSASLQDI 426
Query: 438 VARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTV 497
+ARFE+RSG W++FPEKVAVQMNDTHPTLCIPEL+RIL+D+KGLSW++AW+ITQRTV
Sbjct: 427 IARFEKRSGNAVNWDQFPEKVAVQMNDTHPTLCIPELLRILMDVKGLSWKQAWEITQRTV 486
Query: 498 AYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKE 557
AYTNHTVLPEALEKWS L+ +LLPRHVEII IDEEL+ TI++EYGT DL LL +KL +
Sbjct: 487 AYTNHTVLPEALEKWSFTLLGELLPRHVEIIAMIDEELLHTILAEYGTEDLDLLQEKLNQ 546
Query: 558 LRILENVDLPAAYSDLIIEPEESSV---VASTEEPEISNEVDSADEENSEGQAKSIGEDE 617
+RIL+NV++P++ +L+I+ EES+ A+ EE E + DS DEE +A++ E+E
Sbjct: 547 MRILDNVEIPSSVLELLIKAEESAADVEKAADEEQEEEGKDDSKDEETEAVKAETTNEEE 606
Query: 618 TVE-EEDEPESKGIQAKKV-EPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFN 677
E ++ E E + K++ P P P++V MANLCV GHAVNGVAEIHSEIVKDEVFN
Sbjct: 607 ETEVKKVEVEDSQAKIKRIFGPHPNKPQVVHMANLCVVSGHAVNGVAEIHSEIVKDEVFN 666
Query: 678 AFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNE 737
FYKLWP KFQNKTNGVTPRRW+ FCNP+LS++IT W GS+DW++NTEKL EL+ FADNE
Sbjct: 667 EFYKLWPEKFQNKTNGVTPRRWLSFCNPELSEIITKWTGSDDWLVNTEKLAELRKFADNE 726
Query: 738 DLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVYRY 797
+LQ++WR AK NNK+K VS +KEKTGY VSPDAMFD+Q +KRIHEYKRQLLNI GIVYRY
Sbjct: 727 ELQSEWRKAKGNNKMKIVSLIKEKTGYVVSPDAMFDVQ-IKRIHEYKRQLLNIFGIVYRY 786
Query: 798 KKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVI 857
KKMKEMS EERKE FVPRVCIFGGKAFATYVQAKRIVKFITDVG TVNHDPEIGDLLKV+
Sbjct: 787 KKMKEMSPEERKEKFVPRVCIFGGKAFATYVQAKRIVKFITDVGETVNHDPEIGDLLKVV 846
Query: 858 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEV 917
FVPDYNVSVAE+LIP SELSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGANVEIR+EV
Sbjct: 847 FVPDYNVSVAEVLIPGSELSQHISTAGMEASGTSNMKFSMNGCLLIGTLDGANVEIREEV 906
Query: 918 GAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGN 977
G +NFFLFGA+AHEIAGLRKERAEGKF+PDPRFEEVK ++R+GVFG+Y+YEE++GSLEGN
Sbjct: 907 GEDNFFLFGAQAHEIAGLRKERAEGKFVPDPRFEEVKAFIRTGVFGTYNYEELMGSLEGN 966
Query: 978 EGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAK 1037
EG+GRADYFLVGKDFP YIECQ++VDEAYRDQKKWT+MSILNTAGS+KFSSDRTIH+YA+
Sbjct: 967 EGYGRADYFLVGKDFPDYIECQDKVDEAYRDQKKWTKMSILNTAGSFKFSSDRTIHQYAR 974
BLAST of MS014512 vs. ExPASy TrEMBL
Match:
A0A6J1CTT4 (Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111014157 PE=3 SV=1)
HSP 1 Score: 1908.3 bits (4942), Expect = 0.0e+00
Identity = 974/1037 (93.92%), Postives = 974/1037 (93.92%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS
Sbjct: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY
Sbjct: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE
Sbjct: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDLSAFNAGEHTKASEALASADK
Sbjct: 301 NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA
Sbjct: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ
Sbjct: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV 660
AKSIGEDETVEEEDEPESKGIQ KKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV
Sbjct: 601 AKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIV 660
Query: 661 KDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 720
KDEVFNAFYKLWP KFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK
Sbjct: 661 KDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELK 720
Query: 721 MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNIL 780
MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLLNIL
Sbjct: 721 MFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLLNIL 780
Query: 781 GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 840
GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG
Sbjct: 781 GIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIG 840
Query: 841 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 900
DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV
Sbjct: 841 DLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANV 900
Query: 901 EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI 960
EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI
Sbjct: 901 EIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMI 960
Query: 961 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT 1020
GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT
Sbjct: 961 GSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRT 977
Query: 1021 IHEYAKDIWNIKPVELP 1038
IHEYAKDIWNIKPVELP
Sbjct: 1021 IHEYAKDIWNIKPVELP 977
BLAST of MS014512 vs. ExPASy TrEMBL
Match:
A0A5D3CVD4 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold637G00490 PE=3 SV=1)
HSP 1 Score: 1703.7 bits (4411), Expect = 0.0e+00
Identity = 873/1040 (83.94%), Postives = 914/1040 (87.88%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KLKELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. ExPASy TrEMBL
Match:
A0A1S3C0V0 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo OX=3656 GN=LOC103495729 PE=3 SV=1)
HSP 1 Score: 1701.4 bits (4405), Expect = 0.0e+00
Identity = 872/1040 (83.85%), Postives = 913/1040 (87.79%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KLKELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKE TGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEXTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. ExPASy TrEMBL
Match:
A0A5A7UBY0 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold428G00580 PE=3 SV=1)
HSP 1 Score: 1700.3 bits (4402), Expect = 0.0e+00
Identity = 871/1040 (83.75%), Postives = 913/1040 (87.79%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS
Sbjct: 1 MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EP K DPVAD+ES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61 EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 301 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 361 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 420
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 421 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 481 EEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLPRHVEIIELIDEELIRTIISEYGTA 540
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KL ELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D+EN
Sbjct: 541 DLKLLREKLNELRILENVDLPAAYSDLFIEPEESSTIASTEVLKSSKEADSVDKENLSKL 600
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPIP---PPPKMVRMANLCVAGGHAVNGVAEIHS 660
AK + +DE V E+DE E K IQ KKVEP PPPKMVRMANLCV GGHAVNGVAEIHS
Sbjct: 601 AKFVDKDEFV-EDDEVECKDIQDKKVEPTSPPLPPPKMVRMANLCVVGGHAVNGVAEIHS 660
Query: 661 EIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLG 720
EIVKDEVFN+FYKLWP KFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 661 EIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLG 720
Query: 721 ELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLL 780
LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLL
Sbjct: 721 GLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLL 780
Query: 781 NILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
NI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP
Sbjct: 781 NIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDP 840
Query: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG
Sbjct: 841 EIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDG 900
Query: 901 ANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
ANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE
Sbjct: 901 ANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYE 960
Query: 961 EMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSS 1020
E+IGSLEGNEGFGRADYFLVGKDFPSYIECQ++VDEAYRDQK+WTRMSILNTAGSYKFSS
Sbjct: 961 ELIGSLEGNEGFGRADYFLVGKDFPSYIECQKKVDEAYRDQKRWTRMSILNTAGSYKFSS 976
Query: 1021 DRTIHEYAKDIWNIKPVELP 1038
DRTIHEYAKDIW+IKPVELP
Sbjct: 1021 DRTIHEYAKDIWSIKPVELP 976
BLAST of MS014512 vs. ExPASy TrEMBL
Match:
A0A0A0KXS1 (Alpha-1,4 glucan phosphorylase OS=Cucumis sativus OX=3659 GN=Csa_4G095050 PE=3 SV=1)
HSP 1 Score: 1695.2 bits (4389), Expect = 0.0e+00
Identity = 871/1039 (83.83%), Postives = 910/1039 (87.58%), Query Frame = 0
Query: 1 MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
MAA+SQ T+ LN SFSH+ SFP+L+GLS +Y +SK LL+ TS+WRS +RTF V+NVSS
Sbjct: 57 MAATSQFTLALNPPHSFSHSYSFPSLIGLSSRYRQSKFLLLSTSSWRSPKRTFLVRNVSS 116
Query: 61 EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
EPK K DPVADEES A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 117 EPKLK---DPVADEESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 176
Query: 121 SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 177 SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 236
Query: 181 ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 237 ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 296
Query: 241 VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
VAENWLEIGNPWE+VRNDIKY IKFYGKVVIGSDGKKNWTGGEDIEAVA+DVPIPGYKTK
Sbjct: 297 VAENWLEIGNPWEIVRNDIKYHIKFYGKVVIGSDGKKNWTGGEDIEAVAHDVPIPGYKTK 356
Query: 301 NTINLRLWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
NTINLRLWSTKAP EDFDL+AFNAGEH++ASEALASA+K
Sbjct: 357 NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 416
Query: 361 GLKWLKLIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQ 420
ICHVLYPGDDSIEGKILRLKQQ
Sbjct: 417 --------------------------------------ICHVLYPGDDSIEGKILRLKQQ 476
Query: 421 YTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 480
YTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW
Sbjct: 477 YTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSW 536
Query: 481 EEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTA 540
EEAW +TQRTVAYTNHTVLPEALEKW+ ELMQ+LLPRHVEIIE IDEELI+TIISEYGTA
Sbjct: 537 EEAWNVTQRTVAYTNHTVLPEALEKWNFELMQRLLPRHVEIIELIDEELIRTIISEYGTA 596
Query: 541 DLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQ 600
DLKLL +KLKELRILENVDLPAAYSDL IEPEESS +ASTE + S E DS D
Sbjct: 597 DLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTIASTEVLKRSKEADSVD------- 656
Query: 601 AKSIGEDETVEEEDEPESKGIQAKKVEPI--PPPPKMVRMANLCVAGGHAVNGVAEIHSE 660
+DE VE +DE ESKGIQ KKVEP PPPPKMVRMANL V GGHAVNGVAEIHSE
Sbjct: 657 -----KDEFVEVDDELESKGIQDKKVEPTPPPPPPKMVRMANLSVVGGHAVNGVAEIHSE 716
Query: 661 IVKDEVFNAFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGE 720
IVKDEVFNAFYKLWPGKFQNKTNGVTPRRWI FCNPDLSKLITNWIGSEDWVLNTEKLG
Sbjct: 717 IVKDEVFNAFYKLWPGKFQNKTNGVTPRRWILFCNPDLSKLITNWIGSEDWVLNTEKLGG 776
Query: 721 LKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLN 780
LK FAD+EDLQ QWR AKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ VKRIHEYKRQLLN
Sbjct: 777 LKKFADDEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQ-VKRIHEYKRQLLN 836
Query: 781 ILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPE 840
ILGIVYRYKKMKEMSA+ERKE +VPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPE
Sbjct: 837 ILGIVYRYKKMKEMSAKERKETYVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPE 896
Query: 841 IGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGA 900
IGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGA
Sbjct: 897 IGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGA 956
Query: 901 NVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEE 960
NVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGS DYEE
Sbjct: 957 NVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSCDYEE 1016
Query: 961 MIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSD 1020
+I SLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQKKWTRMSILNTAGSYKFSSD
Sbjct: 1017 LIASLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKKWTRMSILNTAGSYKFSSD 1020
Query: 1021 RTIHEYAKDIWNIKPVELP 1038
RTIHEYAKDIW+IKPVELP
Sbjct: 1077 RTIHEYAKDIWSIKPVELP 1020
BLAST of MS014512 vs. TAIR 10
Match:
AT3G29320.1 (Glycosyl transferase, family 35 )
HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 741/1031 (71.87%), Postives = 837/1031 (81.18%), Query Frame = 0
Query: 14 ADSFSHANSFPTLLGLSHKYGK--SKLLLVRTSNWRSA--RRTF-AVKNVSSEPKQKLKD 73
A+ NS +L+ GK +++ R + R + RR+F +VK++SSEPK K+ D
Sbjct: 13 AEVLIQCNSLSSLVSRRCDDGKWRTRMFPARNRDLRPSPTRRSFLSVKSISSEPKAKVTD 72
Query: 74 DPVADEESSIVAS--AFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDAL 133
+ E+ ++S F PDAAS+ASSIKYHAEFTPLFSP++F+LPKAFFATAQSVRDAL
Sbjct: 73 AVLDSEQEVFISSMNPFAPDAASVASSIKYHAEFTPLFSPEKFELPKAFFATAQSVRDAL 132
Query: 134 IINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVA 193
I+NWNATYE Y ++NVKQAYYLSMEFLQGRAL NA+GNL L AY +AL +LG++LE+VA
Sbjct: 133 IMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGLNSAYGDALKRLGFDLESVA 192
Query: 194 CQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWL 253
QEPD ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ+ITKDGQEE AE+WL
Sbjct: 193 SQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWL 252
Query: 254 EIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLR 313
E+ NPWE+VRND+ YPIKFYGKVV GSDGKK W GGEDI AVAYDVPIPGYKTK TINLR
Sbjct: 253 ELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLR 312
Query: 314 LWSTKAPAEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLK 373
LWSTKAP+EDFDLS++N+G+HT+A+EAL +A+K
Sbjct: 313 LWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEK--------------------------- 372
Query: 374 LIIEFLVRKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSA 433
IC VLYPGD+S EGK LRLKQQYTLCSA
Sbjct: 373 --------------------------------ICFVLYPGDESTEGKALRLKQQYTLCSA 432
Query: 434 SLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKI 493
SLQDIVARFE RSG N WEEFPEKVAVQMNDTHPTLCIPELMRIL+DLKGLSWE+AWKI
Sbjct: 433 SLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWKI 492
Query: 494 TQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLH 553
TQRTVAYTNHTVLPEALEKWSLELM++LLPRHVEIIE+IDEEL++TI+SEYGTAD LL
Sbjct: 493 TQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKIDEELVRTIVSEYGTADPDLLE 552
Query: 554 KKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQAKSIGE 613
+KLK +RILENV+LP+A++D+I++P V A + N V + EE K+ GE
Sbjct: 553 EKLKAMRILENVELPSAFADVIVKPVNKPVTAK----DAQNGVKTEQEEE-----KTAGE 612
Query: 614 DETVEEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFN 673
EEEDE EP PPKMVRMANL V GGHAVNGVAEIHSEIVK +VFN
Sbjct: 613 ----EEEDEVIP--------EPTVEPPKMVRMANLAVVGGHAVNGVAEIHSEIVKQDVFN 672
Query: 674 AFYKLWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNE 733
F +LWP KFQNKTNGVTPRRWIRFCNP LS +ITNWIG+EDWVLNTEK+ EL+ FADNE
Sbjct: 673 DFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAELRKFADNE 732
Query: 734 DLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVYRY 793
DLQ++WR AK+ NKLK VS +KE+TGYTVSPDAMFDIQ +KRIHEYKRQLLNILGIVYRY
Sbjct: 733 DLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFDIQ-IKRIHEYKRQLLNILGIVYRY 792
Query: 794 KKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVI 853
KKMKEMSA ER++AFVPRVCIFGGKAFATYVQAKRIVKFITDV +T+NHDPEIGDLLKVI
Sbjct: 793 KKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEIGDLLKVI 852
Query: 854 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEV 913
FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGANVEIR+EV
Sbjct: 853 FVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGANVEIREEV 912
Query: 914 GAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGN 973
G ENFFLFGAKA +I LRKERAEGKF+PDP FEEVK++V SGVFGS Y+E+IGSLEGN
Sbjct: 913 GEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSNSYDELIGSLEGN 962
Query: 974 EGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAK 1033
EGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTRMSI+NTAGS+KFSSDRTIHEYAK
Sbjct: 973 EGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAK 962
Query: 1034 DIWNIKPVELP 1038
DIWNIK VELP
Sbjct: 1033 DIWNIKQVELP 962
BLAST of MS014512 vs. TAIR 10
Match:
AT3G46970.1 (alpha-glucan phosphorylase 2 )
HSP 1 Score: 1034.2 bits (2673), Expect = 6.9e-302
Identity = 527/967 (54.50%), Postives = 656/967 (67.84%), Query Frame = 0
Query: 74 EESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWNAT 133
E+ S A+ DA IA +I YHA+++P FSP +F +A +ATA+S+RD LI WN T
Sbjct: 14 EKISAKANPEADDATEIAGNIVYHAKYSPHFSPLKFGPEQALYATAESLRDRLIQLWNET 73
Query: 134 YELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPDAA 193
Y + K++ KQ YYLSME+LQGRAL NAIGNL L G YA+AL LGYELE +A QE DAA
Sbjct: 74 YVHFNKVDPKQTYYLSMEYLQGRALTNAIGNLNLQGPYADALRTLGYELEEIAEQEKDAA 133
Query: 194 LGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNPWE 253
LGNGGLGRLASCFLDS+ATLN PAWGYGLRYR+GLFKQ ITK GQEE+ E+WLE +PWE
Sbjct: 134 LGNGGLGRLASCFLDSMATLNLPAWGYGLRYRHGLFKQIITKKGQEEIPEDWLEKFSPWE 193
Query: 254 VVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTKAP 313
+VR+D+ +P++F+GKV + DG + W G+ ++A+AYDVPIPGY TKNTI+LRLW KA
Sbjct: 194 IVRHDVVFPVRFFGKVQVNPDGSRKWVDGDVVQALAYDVPIPGYGTKNTISLRLWEAKAR 253
Query: 314 AEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLV 373
AED DL FN GE+ A++ + A
Sbjct: 254 AEDLDLFQFNEGEYELAAQLHSRAQ----------------------------------- 313
Query: 374 RKIENFMKRICTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVA 433
QIC VLYPGD + GK+LRLKQQ+ LCSASLQDI++
Sbjct: 314 ------------------------QICTVLYPGDATENGKLLRLKQQFFLCSASLQDIIS 373
Query: 434 RFERRS--GANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTV 493
RF RS ++KW EFP KVAVQMNDTHPTL IPELMR+L+D GL W+EAW +T +TV
Sbjct: 374 RFHERSTTEGSRKWSEFPSKVAVQMNDTHPTLAIPELMRLLMDDNGLGWDEAWDVTSKTV 433
Query: 494 AYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKL-LHKKLK 553
AYTNHTVLPEALEKWS LM +LLPRH+EIIE+ID+ +QTI D ++ L K+
Sbjct: 434 AYTNHTVLPEALEKWSQSLMWKLLPRHMEIIEEIDKRFVQTI------RDTRVDLEDKIS 493
Query: 554 ELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQAKSIGEDETV 613
L IL+N
Sbjct: 494 SLSILDN----------------------------------------------------- 553
Query: 614 EEEDEPESKGIQAKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYK 673
P +VRMANLCV H VNGVA++HS+I+K E+F +
Sbjct: 554 -------------------NPQKPVVRMANLCVVSSHTVNGVAQLHSDILKAELFADYVS 613
Query: 674 LWPGKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQT 733
+WP KFQNKTNG+TPRRW+RFC+P+LS +IT W+ ++ W+ + + L L+ FADNE+LQ+
Sbjct: 614 IWPNKFQNKTNGITPRRWLRFCSPELSDIITKWLKTDKWITDLDLLTGLRQFADNEELQS 673
Query: 734 QWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVVKRIHEYKRQLLNILGIVYRYKKMK 793
+W +AK NK + +++ TG ++ P ++FDIQ VKRIHEYKRQL+NILG+VYR+KK+K
Sbjct: 674 EWASAKTANKKRLAQYIERVTGVSIDPTSLFDIQ-VKRIHEYKRQLMNILGVVYRFKKLK 733
Query: 794 EMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPD 853
EM EERK+ VPR + GGKAFATY AKRIVK + DVG VN DPE+ + LKV+FVP+
Sbjct: 734 EMKPEERKKT-VPRTVMIGGKAFATYTNAKRIVKLVNDVGDVVNSDPEVNEYLKVVFVPN 793
Query: 854 YNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAEN 913
YNV+VAE+LIP SELSQHISTAGMEASGTSNMKFA+NGC++IGTLDGANVEIR+EVG EN
Sbjct: 794 YNVTVAEMLIPGSELSQHISTAGMEASGTSNMKFALNGCLIIGTLDGANVEIREEVGEEN 841
Query: 914 FFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFG 973
FFLFGA A ++ LRKER +G F PDPRFEE K++V+SGVFGSYDY ++ SLEGN GFG
Sbjct: 854 FFLFGATADQVPRLRKEREDGLFKPDPRFEEAKQFVKSGVFGSYDYGPLLDSLEGNTGFG 841
Query: 974 RADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWN 1033
R DYFLVG DFPSY++ Q +VDEAY+D+K W +MSIL+TAGS KFSSDRTI +YAK+IWN
Sbjct: 914 RGDYFLVGYDFPSYMDAQAKVDEAYKDRKGWLKMSILSTAGSGKFSSDRTIAQYAKEIWN 841
Query: 1034 IKPVELP 1038
I+ +P
Sbjct: 974 IEACPVP 841
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022144477.1 | 0.0e+00 | 93.92 | alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic [Momordica ... | [more] |
XP_038895156.1 | 0.0e+00 | 85.34 | alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Benincas... | [more] |
TYK15871.1 | 0.0e+00 | 83.94 | alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa] | [more] |
XP_008455590.1 | 0.0e+00 | 83.85 | PREDICTED: LOW QUALITY PROTEIN: alpha-1,4 glucan phosphorylase L-1 isozyme, chlo... | [more] |
KAA0053393.1 | 0.0e+00 | 83.75 | alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa] | [more] |
Match Name | E-value | Identity | Description | |
P53536 | 0.0e+00 | 72.77 | Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia fa... | [more] |
P04045 | 0.0e+00 | 71.65 | Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanu... | [more] |
P27598 | 0.0e+00 | 73.07 | Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea ... | [more] |
Q9LIB2 | 0.0e+00 | 71.87 | Alpha-glucan phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=PHS1 PE=1 SV=1 | [more] |
P53535 | 0.0e+00 | 69.16 | Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanu... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CTT4 | 0.0e+00 | 93.92 | Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111014157 PE... | [more] |
A0A5D3CVD4 | 0.0e+00 | 83.94 | Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3C0V0 | 0.0e+00 | 83.85 | Alpha-1,4 glucan phosphorylase OS=Cucumis melo OX=3656 GN=LOC103495729 PE=3 SV=1 | [more] |
A0A5A7UBY0 | 0.0e+00 | 83.75 | Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... | [more] |
A0A0A0KXS1 | 0.0e+00 | 83.83 | Alpha-1,4 glucan phosphorylase OS=Cucumis sativus OX=3659 GN=Csa_4G095050 PE=3 S... | [more] |