MS014493 (gene) Bitter gourd (TR) v1

Overview


Name	MS014493
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	scaffold553: 370791 .. 373640 (-)
RNA-Seq Expression	MS014493
Synteny	MS014493

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAACTTGCTAATTGACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAATCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGCTTGAATATTTGTTCGCTTACTGTGGACTTCATGCGCTCCAGGTTTGATATGTGATACTTTAGAGCCTGTTTAGTTATAATCCTCGTTATAAATCTAATGTTTTTGAATTTTGTGATAAAAAGTTTGAAAACAACATTTTATGTTTTCATTCTTTTCAAAATTTAAATTTGAAATTAGAGAAATAAAAATATTGATAGATATACTATTTCATTTTATAAACTCATTTCTTTTTTCTTTTTTCCTTTTTTTAATTAAAACATGTATAATATAATATATAATATCGTAAATAATAATTATACAAAATTTATAACATATATGTTAATAAATTAAACTAATAATACTTTAGATTATGCTCAATATTTAGTTGGTAGCTACTGCTTATTTTCAAATGTTATTACTTAAATAAATTTTTAAAATGAGATTCAATTTCCAGATTTGCTATCAAACACATATATTAAAACACTAAAAACATGAAATACAACTATTTTTCAATGAATCCCTTGTTTTTTCATATTTCAATTTTCACATCACCCGTCAAACACGCTTTACCTTTTACTTTTATAATGAATAGAGAAATTTTGAAGTATTTATCTTTTGAATTTTTTCTTTTTTTGGATTTTAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGCATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGGTAAAATAAAACCTAATATTCTGTTTTATTTGCTTTCAAGTACCAATCAAATGGATAATATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACATACTTTAAAAAAATTAATTTAAATTGAATTAAATAAGACTCCATCTCGAAATAAATTTATACTTCGTTTTTTTTTGTTGTTAGAAATTGGGTTGAATCATTAATAATCTCTTTACTAGTTTTTAAGCATATGAAACCTAAATAGGATCAGTGGTTTAATTAATTATAAGCAAAATATATAGAATATACGAACCATTTCTTTTTTTAGCTATAATAATATACCAACTAAATGAAGGACAAGTATATCACCTAAATTACAATTATACGTTACATTTTTTAAATTAATAATCAAGGATATAGTATAGTTAATAAAAATTAAAACAAAGATCAAAATATGATATATTGATCTACTCCTTTTCACGCAGTTTGTGAGGACTGTAATGCTTTTACATTCGACGTTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGAGTGTGTATTTTTTTTAGAGTGAAAATAATTTAAATAGTTGATTGTAATTGTGATCTTTATTCCAATTATATTTATTAAATGGTAAGTTAATTGAATGATTTTTAGGTCAAATTTAAAACTGACAATTAATATATATATTAAAATGTAGGGGTAGTTGCAAATATGACAAAAATATTTAGTAAGCTAAGATCGGCAAAATAGCCAACCACATTTAGTTTTTTAAATATAGCACTTTTGAAAAAAATAATAGAGATCAAATTTTAAAATGACAACTTACCCAAATGATATATATCTGTAATATATCTATAATATATCTATAATATATATAACTACCGATTTATGTATGTGATATATATATGATATAGTACTGGCATGAATGTCTTATAATATACTTGCAATATATATGTGATATATATTTGATGTACTACTGATATGAGTGTTTGTACAATATGTATGTGATATACATTTGATATATTGTTGATGTGAGTCTGTGATATACTTAACTCATATAAGTGAAAATTTGTTTTATATTTGAAAATTTTAAAATACATGTAATATATTTTGAAATACATAAAATATTTTTTATTTAAATGACTGATATGATTATTTTTTTTTCAGTCACTTTATTTATATATATAGTGATTACTCAATGTAGCATTTATTCAATGTTGCATTGTAATATTATGTGATAATTTAATTCATAGAGTTATGAAATGTTGCATTTATAAAATAATCAAATACTTTTTAAAGAAAAATAAAAGAGTGTATTTTGCAGTTTATTGTAGTTCTTTTTGTATACTTCTTAGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTCCTCAAGACTATTGGAGTGGCCACTGACGTGAAATTACCTCGTATGAGTTCGACTATAGAAGCTTTCAACATACATCCGAAGACAAATATTTCA

mRNA sequence

AAGAACTTGCTAATTGACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAATCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGCTTGAATATTTGTTCGCTTACTGTGGACTTCATGCGCTCCAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGCATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGTTTGTGAGGACTGTAATGCTTTTACATTCGACGTTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTCCTCAAGACTATTGGAGTGGCCACTGACGTGAAATTACCTCGTATGAGTTCGACTATAGAAGCTTTCAACATACATCCGAAGACAAATATTTCA

Coding sequence (CDS)

AAGAACTTGCTAATTGACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAATCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGCTTGAATATTTGTTCGCTTACTGTGGACTTCATGCGCTCCAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGCATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGTTTGTGAGGACTGTAATGCTTTTACATTCGACGTTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTCCTCAAGACTATTGGAGTGGCCACTGACGTGAAATTACCTCGTATGAGTTCGACTATAGAAGCTTTCAACATACATCCGAAGACAAATATTTCA

Protein sequence

KNLLIDESKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSSTIEAFNIHPKTNIS

Homology

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BLAST of MS014493 vs. NCBI nr
Match: XP_022144458.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 646.0 bits (1665), Expect = 1.7e-181
Identity = 302/306 (98.69%), Postives = 302/306 (98.69%), Query Frame = 0


Query: 15  MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 74
           MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS
Sbjct: 1   MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 60

Query: 75  HKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITW 134
           HKSFKLPKW EYL AYCGLHALQGDPIDWVSTHRYHHQFVDTERDP SPTQGLLFSYITW
Sbjct: 61  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPDSPTQGLLFSYITW 120

Query: 135 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 194
           AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI
Sbjct: 121 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 180

Query: 195 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 254
           ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI
Sbjct: 181 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 240

Query: 255 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSSTIEAFNIH 314
           SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMS TIEAFNIH
Sbjct: 241 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFNIH 300

Query: 315 PKTNIS 321
           PKTNIS
Sbjct: 301 PKTNIS 306

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BLAST of MS014493 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 470.7 bits (1210), Expect = 1.0e-128
Identity = 215/308 (69.81%), Postives = 250/308 (81.17%), Query Frame = 0


Query: 5   IDESKQEPKEMAK-------GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALY 64
           ++ SKQEPKEM K        +WT +D  +AC  F VH+ CI APF FNW AF+VAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 65  FITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTE 124
            +TGLFG+++SYHRNL+HKSFKLPKWLEYLFAYCG+ ALQGDP+DWVSTHR HHQFVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 125 RDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLE 184
           RDPHSP +G  FS+I W FDSY LTKKVCPKYFS+F++ ER IFV+  KHG+P NV DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 185 KQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQ 244
           KQAFYRFIH+TYLLH I LAVILYVVGGIPFL+WG  VR V++LH T MV+S+CH WG Q
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 245 QWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVAT 304
            W    +SKNNWW++ I+FGE WHNNHHAFEYSA+ G EWWQ+DFGWY+ILFL+ IGVAT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

Query: 305 DVKLPRMS 306
            VKLP  S
Sbjct: 301 HVKLPSQS 308

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BLAST of MS014493 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-127
Identity = 210/307 (68.40%), Postives = 250/307 (81.43%), Query Frame = 0


Query: 4   LIDESKQEPKEMA--------KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFA 63
           L++  KQEPKEMA        + +WT +D  +AC  FFVH+  +FAPF+FNW AF++AFA
Sbjct: 20  LMEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFA 79

Query: 64  LYFITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVD 123
           LY  TGLFG+S+SYHRNL+HKSF LPKWLEYLFAYCG HALQGDPIDWV+THR HH+FVD
Sbjct: 80  LYVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVD 139

Query: 124 TERDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGD 183
           TERDPHSP QG  FS+ITW  DSY+LTK+VCPKYF+DF++ ER IFVM  KHGRP+NVGD
Sbjct: 140 TERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGD 199

Query: 184 LEKQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWG 243
           LEKQ FYRFIHKTY LH I LA+ILY  GG PFLIWG  VR V++LH T M++S+CH WG
Sbjct: 200 LEKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWG 259

Query: 244 HQQWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGV 303
            Q W    LS+NNWW++ +SFGE WHNNHHAFEYSAR G EWWQIDFGWY+++FL+ IG+
Sbjct: 260 KQPWRTGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGI 319

[Close]

BLAST of MS014493 vs. NCBI nr
Match: XP_038904662.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 466.5 bits (1199), Expect = 1.9e-127
Identity = 212/308 (68.83%), Postives = 246/308 (79.87%), Query Frame = 0


Query: 5   IDESKQEPKEMAK-------GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALY 64
           +D SKQE  E  K        EWT+ID  +AC+ F +H  CIFAPF F WGAF++A  LY
Sbjct: 1   MDVSKQELNERVKPTMSPRRREWTNIDKTIACLLFILHTLCIFAPFQFTWGAFWIALVLY 60

Query: 65  FITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTE 124
            ITGLFG++ISYHRNLSH+SFKLPKWLEYLFAYCG HALQGDPIDWVSTHR HHQFVDT+
Sbjct: 61  VITGLFGITISYHRNLSHQSFKLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHQFVDTD 120

Query: 125 RDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLE 184
            DPHSP QG  +S+ITW  DSY LTKKVC KYF+D +E ER +FV+  KH RP+NV DLE
Sbjct: 121 NDPHSPIQGFWYSHITWVLDSYNLTKKVCRKYFNDSKEAERNLFVLVMKHERPDNVKDLE 180

Query: 185 KQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQ 244
           KQ FY FIHKTY+LH IALAV+L +VGG+PF++WG  VR VML H  FMV+S+CHKWG Q
Sbjct: 181 KQIFYTFIHKTYILHPIALAVLLSMVGGVPFVLWGMCVRIVMLYHVIFMVNSVCHKWGKQ 240

Query: 245 QWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVAT 304
           QW    LS+NNWW++F+SFGE WHNNHHAFEYSA+ G EWWQ+DFGWY+I+FLK IGVAT
Sbjct: 241 QWNTNDLSRNNWWVSFLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKVIGVAT 300

Query: 305 DVKLPRMS 306
           DVKLP  S
Sbjct: 301 DVKLPTQS 308

[Close]

BLAST of MS014493 vs. NCBI nr
Match: XP_038903746.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 464.5 bits (1194), Expect = 7.2e-127
Identity = 209/308 (67.86%), Postives = 246/308 (79.87%), Query Frame = 0


Query: 5   IDESKQEPKEMAK-------GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALY 64
           +D SKQEP E+ K       G+W +ID   A + FF+H+PCIF PF F WGAF+VAF LY
Sbjct: 1   MDVSKQEPSELVKPLTPFERGKWNNIDKTTAFVIFFIHIPCIFGPFQFTWGAFWVAFVLY 60

Query: 65  FITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTE 124
            ITGLFG++ISYHR+LSHKSFKLPKWLEYLFAYCG+HALQGDP+DWVSTHR HHQFVDTE
Sbjct: 61  VITGLFGITISYHRHLSHKSFKLPKWLEYLFAYCGVHALQGDPMDWVSTHRCHHQFVDTE 120

Query: 125 RDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLE 184
           +DPHSP +G  FS+I W  DSY LTKKVCPKYF DF+  ER + ++  KHGRP NV DLE
Sbjct: 121 KDPHSPIEGFWFSHIAWTLDSYLLTKKVCPKYFRDFKGMERNMLMLNLKHGRPTNVVDLE 180

Query: 185 KQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQ 244
           KQ+FYRFI KTY LH IALAV+LY +GGIPF+IWG FVR  M +H  FMV+SI H+WG +
Sbjct: 181 KQSFYRFIQKTYFLHPIALAVVLYAIGGIPFVIWGMFVRVTMFVHVMFMVNSIGHRWGTR 240

Query: 245 QWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVAT 304
           QW  + LS NNWW++ +SFG+ WHNNHHAFEYSAR G EWWQIDF WY+I+FL+ IGVAT
Sbjct: 241 QWNTSDLSTNNWWLSLLSFGDGWHNNHHAFEYSARHGLEWWQIDFSWYVIMFLQAIGVAT 300

Query: 305 DVKLPRMS 306
           +VKLP  S
Sbjct: 301 EVKLPTQS 308

[Close]

BLAST of MS014493 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 1.6e-97
Identity = 162/294 (55.10%), Postives = 203/294 (69.05%), Query Frame = 0


Query: 9   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSIS 68
           K+E     + EW  +D     +   +H+  + APF FNW A  VAF LY +TGL G+++S
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 69  YHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLL 128
           +HRNLSHK+FKLPKWLEYLFAYCG  ALQG+PIDWVSTHRYHHQF D++RDPHSP  G  
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 129 FSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKT 188
           FS++ W FD+  +T++                       G PNNVGDLEKQ FYRF+  T
Sbjct: 207 FSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTT 266

Query: 189 YLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNN 248
           Y+LH +ALAV LY +GG PF++WG  VR V + H T++V+S CH WG Q W    LSKNN
Sbjct: 267 YILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 326

Query: 249 WWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           WW+  ++FGE WHNNHHAFE+SAR G EWWQ+D  WY++ FL+ IG+ATDVKLP
Sbjct: 327 WWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of MS014493 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.9e-86
Identity = 151/307 (49.19%), Postives = 198/307 (64.50%), Query Frame = 0


Query: 1   KNLLIDESKQEPKEMAKGE--WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYF 60
           K +  D+++   K+ A  E  W  +DI  A    FVH  C+ APF+F W A  VA  +Y 
Sbjct: 13  KKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYT 72

Query: 61  ITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTER 120
           + GL G+++SYHRNL+H+SFK+PKWLEY FAYCGL A+QGDPIDWVSTHRYHHQF D++R
Sbjct: 73  VGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDR 132

Query: 121 DPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEK 180
           DPHSP +G  FS++ W FD+  L +K                       GR  NV DL++
Sbjct: 133 DPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKR 192

Query: 181 QAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQ 240
           Q +Y+F+ +T L H++    +LY  GG+ FL WG  +   M  H T +++S+CH WG + 
Sbjct: 193 QWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRT 252

Query: 241 WGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATD 300
           W     S+N WW++  SFGE+WHNNHHAFE SAR G EWWQID  WYI+ FL+ IG+ATD
Sbjct: 253 WKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATD 296

Query: 301 VKLPRMS 306
           VKLP  S
Sbjct: 313 VKLPSES 296

[Close]

BLAST of MS014493 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-85
Identity = 147/283 (51.94%), Postives = 194/283 (68.55%), Query Frame = 0


Query: 20  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 79
           W   DI    +   +HV C+ APF F+W AF+++  LY + G+FG ++SYHRNL+H+SFK
Sbjct: 37  WNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFK 96

Query: 80  LPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITWAFDSY 139
           LPK+LEY FAY GLHALQGDP+ WVSTHRYHH+F DT  DPHSP +G  FS+I W FDS 
Sbjct: 97  LPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDS- 156

Query: 140 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 199
                   KY             +  + GR  N GDL KQ++YRF+ +T++ HV   A +
Sbjct: 157 --------KY-------------ILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAAL 216

Query: 200 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 259
           LY+ GG PF++WG  VRTV   H++++V+S+CH++GHQ W    LS NNW++  ++ GE 
Sbjct: 217 LYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEG 276

Query: 260 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           WHNNHHAFEYSAR G EWWQID  WY+I  L+ +G+ATDVK+P
Sbjct: 277 WHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVP 297

[Close]

BLAST of MS014493 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-85
Identity = 145/284 (51.06%), Postives = 194/284 (68.31%), Query Frame = 0


Query: 19  EWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSF 78
           +W   D+        +HV C+FAP  F+W +F+V FALY I G+FG ++S+HRNL+H+SF
Sbjct: 39  KWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSF 98

Query: 79  KLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITWAFDS 138
           KLPK+LEY FAY GLHALQGDP+ WVSTHRYHH++ DT  DPHSP +G  F +I W FDS
Sbjct: 99  KLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDS 158

Query: 139 YALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAV 198
                    KY             +  K GR  N GDL KQ++YRF+ +T++ HV   A 
Sbjct: 159 ---------KY-------------IIEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQAA 218

Query: 199 ILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGE 258
           +LY+ GG PF++WG  VRT++  H +++V+S+CH+WG++ W    LS NNW++  ++ GE
Sbjct: 219 LLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGE 278

Query: 259 NWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
            WHNNHHAFEYSAR G EWWQID  WYII  L+ +G+ATD+K+P
Sbjct: 279 GWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVP 300

[Close]

BLAST of MS014493 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.7e-83
Identity = 148/295 (50.17%), Postives = 193/295 (65.42%), Query Frame = 0


Query: 9   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALY-FITGLFGLSI 68
           +++ K   + +W   DI+ A     +H     APF+F+W AF+++F L  F +G+ G+++
Sbjct: 70  ERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITL 129

Query: 69  SYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGL 128
            +HR L+H  FKLPK +EY FAYCG  ALQGDP++WVS HRYHHQFVDTERD HSPTQG 
Sbjct: 130 CFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGF 189

Query: 129 LFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHK 188
            F +I W  D              D   E+RG        GR NNV DL+KQAFYRF+ K
Sbjct: 190 WFCHIGWVLD-------------KDLFVEKRG--------GRRNNVNDLKKQAFYRFLQK 249

Query: 189 TYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKN 248
           TY+ H +AL  +LY VGG P+++WG   R V + HSTF ++S+CHKWG + W    LS N
Sbjct: 250 TYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTN 309

Query: 249 NWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           N ++   +FGE WHNNHHAFE SAR G EWW+ID  WY+I  L+ IG+AT+VKLP
Sbjct: 310 NMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLP 343

[Close]

BLAST of MS014493 vs. ExPASy TrEMBL
Match: A0A6J1CTB0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014139 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 8.4e-182
Identity = 302/306 (98.69%), Postives = 302/306 (98.69%), Query Frame = 0


Query: 15  MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 74
           MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS
Sbjct: 1   MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 60

Query: 75  HKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITW 134
           HKSFKLPKW EYL AYCGLHALQGDPIDWVSTHRYHHQFVDTERDP SPTQGLLFSYITW
Sbjct: 61  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPDSPTQGLLFSYITW 120

Query: 135 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 194
           AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI
Sbjct: 121 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 180

Query: 195 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 254
           ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI
Sbjct: 181 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 240

Query: 255 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSSTIEAFNIH 314
           SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMS TIEAFNIH
Sbjct: 241 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFNIH 300

Query: 315 PKTNIS 321
           PKTNIS
Sbjct: 301 PKTNIS 306

[Close]

BLAST of MS014493 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.9e-129
Identity = 215/308 (69.81%), Postives = 250/308 (81.17%), Query Frame = 0


Query: 5   IDESKQEPKEMAK-------GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALY 64
           ++ SKQEPKEM K        +WT +D  +AC  F VH+ CI APF FNW AF+VAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 65  FITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTE 124
            +TGLFG+++SYHRNL+HKSFKLPKWLEYLFAYCG+ ALQGDP+DWVSTHR HHQFVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 125 RDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLE 184
           RDPHSP +G  FS+I W FDSY LTKKVCPKYFS+F++ ER IFV+  KHG+P NV DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 185 KQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQ 244
           KQAFYRFIH+TYLLH I LAVILYVVGGIPFL+WG  VR V++LH T MV+S+CH WG Q
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 245 QWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVAT 304
            W    +SKNNWW++ I+FGE WHNNHHAFEYSA+ G EWWQ+DFGWY+ILFL+ IGVAT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

Query: 305 DVKLPRMS 306
            VKLP  S
Sbjct: 301 HVKLPSQS 308

[Close]

BLAST of MS014493 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.0e-128
Identity = 210/307 (68.40%), Postives = 250/307 (81.43%), Query Frame = 0


Query: 4   LIDESKQEPKEMA--------KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFA 63
           L++  KQEPKEMA        + +WT +D  +AC  FFVH+  +FAPF+FNW AF++AFA
Sbjct: 20  LMEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFA 79

Query: 64  LYFITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVD 123
           LY  TGLFG+S+SYHRNL+HKSF LPKWLEYLFAYCG HALQGDPIDWV+THR HH+FVD
Sbjct: 80  LYVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVD 139

Query: 124 TERDPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGD 183
           TERDPHSP QG  FS+ITW  DSY+LTK+VCPKYF+DF++ ER IFVM  KHGRP+NVGD
Sbjct: 140 TERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGD 199

Query: 184 LEKQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWG 243
           LEKQ FYRFIHKTY LH I LA+ILY  GG PFLIWG  VR V++LH T M++S+CH WG
Sbjct: 200 LEKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWG 259

Query: 244 HQQWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGV 303
            Q W    LS+NNWW++ +SFGE WHNNHHAFEYSAR G EWWQIDFGWY+++FL+ IG+
Sbjct: 260 KQPWRTGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGI 319

[Close]

BLAST of MS014493 vs. ExPASy TrEMBL
Match: A0A6J1GXQ0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457788 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.8e-124
Identity = 211/320 (65.94%), Postives = 245/320 (76.56%), Query Frame = 0


Query: 8   SKQEPK-------EMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 67
           SKQE K       +    EWT  +  +A   F VH+ CIFAP  F W AF+VA ALY IT
Sbjct: 4   SKQESKDNMEPAMQFCSREWTIREKFVAFYIFIVHILCIFAPSQFTWNAFWVAIALYVIT 63

Query: 68  GLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 127
           GLFG+SISYHRNLSH+SF LPKWLEYLFAYCG+HA QGDPIDWVSTHR HHQ+ DTE DP
Sbjct: 64  GLFGISISYHRNLSHRSFNLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQYADTEGDP 123

Query: 128 HSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 187
           HSPTQG  FSYITW FDS  LTKKVCP+YF D ++++R +F +  K+GRP+NVGDLEKQ 
Sbjct: 124 HSPTQGFWFSYITWLFDSITLTKKVCPEYFIDHKDKKRSVFTLVLKYGRPDNVGDLEKQT 183

Query: 188 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 247
           FYRFIH TY+LH+IALA++LYVVGG PFL+WG  VR VM LHSTFMV+SICH WG QQW 
Sbjct: 184 FYRFIHDTYVLHLIALAILLYVVGGTPFLVWGMGVRIVMSLHSTFMVNSICHSWGKQQWQ 243

Query: 248 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 307
              L++NNWW++ ISFGE WHNNHHAFEYSAR+G EWWQ+D GWY+I FL+ IGVATDVK
Sbjct: 244 TKDLTRNNWWLSLISFGEAWHNNHHAFEYSARVGIEWWQLDLGWYVIWFLQAIGVATDVK 303

Query: 308 LPRMSSTIEAFNIHPKTNIS 321
            P  S        +PK  +S
Sbjct: 304 QPSESHKQRLAMDNPKEGVS 323

[Close]

BLAST of MS014493 vs. ExPASy TrEMBL
Match: A0A6J1E0E4 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111024778 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.4e-123
Identity = 209/301 (69.44%), Postives = 243/301 (80.73%), Query Frame = 0


Query: 8   SKQEPKEMA---KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFG 67
           SK+EP +M    K EW+ ID +MA  Y F+HV CIFAPFHFNW AF+VAFAL+ IT LFG
Sbjct: 4   SKEEPTKMVKLFKREWSMIDKEMAFFYVFIHVVCIFAPFHFNWSAFWVAFALHIITCLFG 63

Query: 68  LSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPT 127
           +SISYHRNLSH+SF+LP+WLEYLFAYCG+HALQGDPI WVSTHR HHQFVDTE+DPHSP 
Sbjct: 64  ISISYHRNLSHRSFQLPRWLEYLFAYCGVHALQGDPIGWVSTHRSHHQFVDTEKDPHSPI 123

Query: 128 QGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRF 187
           QG  FS++TW FDS +LTKKVCPKYF +FEE +R   V+  K+GRP+NV DLEKQ FYRF
Sbjct: 124 QGFWFSHLTWMFDSNSLTKKVCPKYFDNFEEVKRSGCVIVGKYGRPDNVSDLEKQTFYRF 183

Query: 188 IHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGL 247
           I KTYLLH IALA +LY +GG+PFLIWG  VRT+  LH TFMV+S+CH WG Q W N  L
Sbjct: 184 IQKTYLLHPIALAFLLYALGGLPFLIWGMCVRTITCLHITFMVNSVCHIWGKQPWKNGDL 243

Query: 248 SKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRM 306
           S+NN W+  + FGE WHNNHHAFEYSAR G EWWQIDFGWY+I  L+ IGVAT+VK+P  
Sbjct: 244 SRNNGWVGLLGFGEGWHNNHHAFEYSARFGLEWWQIDFGWYVIRLLQAIGVATNVKVPSQ 303

[Close]

BLAST of MS014493 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-98
Identity = 162/294 (55.10%), Postives = 203/294 (69.05%), Query Frame = 0


Query: 9   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSIS 68
           K+E     + EW  +D     +   +H+  + APF FNW A  VAF LY +TGL G+++S
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 69  YHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLL 128
           +HRNLSHK+FKLPKWLEYLFAYCG  ALQG+PIDWVSTHRYHHQF D++RDPHSP  G  
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 129 FSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKT 188
           FS++ W FD+  +T++                       G PNNVGDLEKQ FYRF+  T
Sbjct: 207 FSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTT 266

Query: 189 YLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNN 248
           Y+LH +ALAV LY +GG PF++WG  VR V + H T++V+S CH WG Q W    LSKNN
Sbjct: 267 YILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 326

Query: 249 WWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           WW+  ++FGE WHNNHHAFE+SAR G EWWQ+D  WY++ FL+ IG+ATDVKLP
Sbjct: 327 WWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of MS014493 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 319.3 bits (817), Expect = 3.5e-87
Identity = 151/307 (49.19%), Postives = 198/307 (64.50%), Query Frame = 0


Query: 1   KNLLIDESKQEPKEMAKGE--WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYF 60
           K +  D+++   K+ A  E  W  +DI  A    FVH  C+ APF+F W A  VA  +Y 
Sbjct: 13  KKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYT 72

Query: 61  ITGLFGLSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTER 120
           + GL G+++SYHRNL+H+SFK+PKWLEY FAYCGL A+QGDPIDWVSTHRYHHQF D++R
Sbjct: 73  VGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDR 132

Query: 121 DPHSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEK 180
           DPHSP +G  FS++ W FD+  L +K                       GR  NV DL++
Sbjct: 133 DPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKR 192

Query: 181 QAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQ 240
           Q +Y+F+ +T L H++    +LY  GG+ FL WG  +   M  H T +++S+CH WG + 
Sbjct: 193 QWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRT 252

Query: 241 WGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATD 300
           W     S+N WW++  SFGE+WHNNHHAFE SAR G EWWQID  WYI+ FL+ IG+ATD
Sbjct: 253 WKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATD 296

Query: 301 VKLPRMS 306
           VKLP  S
Sbjct: 313 VKLPSES 296

[Close]

BLAST of MS014493 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 308.1 bits (788), Expect = 8.0e-84
Identity = 145/298 (48.66%), Postives = 188/298 (63.09%), Query Frame = 0


Query: 5   IDESKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFG 64
           ++E K+         W  +D       F VH   + APF+F W A +V F  Y I GL G
Sbjct: 21  VEEKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-G 80

Query: 65  LSISYHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPT 124
           +++SYHRNL+H+SFK+PKWLEYL AYC L A+QGDPIDWVSTHRYHHQF D+ERDPHSP 
Sbjct: 81  ITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPK 140

Query: 125 QGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRF 184
           +G  FS++ W +DS  L  K                       GR  NV DL++Q FYRF
Sbjct: 141 EGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKRQWFYRF 200

Query: 185 IHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGL 244
           + KT L H++ L   L+ +GG+ F+ WG  V   + +H T +++S+CH WG + W     
Sbjct: 201 LQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDT 260

Query: 245 SKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           S+N WW++  SFGE+WHNNHHAFE SAR G EWWQID  WYI+ F + IG+ATDVK+P
Sbjct: 261 SRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

[Close]

BLAST of MS014493 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 301.6 bits (771), Expect = 7.5e-82
Identity = 139/283 (49.12%), Postives = 187/283 (66.08%), Query Frame = 0


Query: 20  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 79
           WT  D+    I+   H+  + APF+F+W AF+V   L FI G+  +++SYHRNLSH+SF 
Sbjct: 91  WTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSYHRNLSHRSFD 150

Query: 80  LPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLLFSYITWAFDSY 139
           LPKWLEYLFAY G+ A QGDPI+WVS HRYHH+  +T+RDPHSPTQG  FS++ W FD+ 
Sbjct: 151 LPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWIFDTS 210

Query: 140 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 199
           ++ +                        G   NV DL +Q FYRF+ +T LLH++A + +
Sbjct: 211 SILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFL 270

Query: 200 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 259
            Y  GG+P L+WG  +   + LH TF+V+S+CH WG + W  +  SKNNWW+  ++ GE 
Sbjct: 271 FYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEG 330

Query: 260 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           WHNNHHAFE+SAR G EWWQ+D  W +I FL+ IG+AT+VKLP
Sbjct: 331 WHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLP 350

[Close]

BLAST of MS014493 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-76
Identity = 137/294 (46.60%), Postives = 187/294 (63.61%), Query Frame = 0


Query: 9   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSIS 68
           ++E +     +WT  D+  A     VH+ C+ APF++ W AF     L  +T L  ++ S
Sbjct: 17  RKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC-ITFS 76

Query: 69  YHRNLSHKSFKLPKWLEYLFAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPHSPTQGLL 128
           YHRNL+H+SFKLPKWLEY FAY  L ALQGDP+DWVS HR+HHQF D++RDPHSP +G  
Sbjct: 77  YHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFW 136

Query: 129 FSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKT 188
           FS++ W FD             +D+  E         K GR NNV DL++Q FYRF+ KT
Sbjct: 137 FSHVLWIFD-------------TDYIRE---------KCGRRNNVMDLKQQWFYRFLKKT 196

Query: 189 YLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNN 248
            +LH++A   ++Y+ GG+P+L W      V+  H T++V+S CH  G Q W     S+N 
Sbjct: 197 LVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNV 256

Query: 249 WWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 303
           WW+  ++ GE+WHNNHHAFE SAR G EW+Q+D  WY+I F + +G+AT+VKLP
Sbjct: 257 WWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLP 287

The following BLAST results are available for this feature:

BLAST of MS014493 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144458.1	1.7e-181	98.69	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153644.1	1.0e-128	69.81	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153632.1	1.5e-127	68.40	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_038904662.1	1.9e-127	68.83	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_038903746.1	7.2e-127	67.86	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]

BLAST of MS014493 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	1.6e-97	55.10	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	4.9e-86	49.19	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
P0DOW3	1.9e-85	51.94	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]
P0DOW2	4.2e-85	51.06	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9FV68	8.7e-83	50.17	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]

BLAST of MS014493 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTB0	8.4e-182	98.69	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DJP0	4.9e-129	69.81	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DHZ7	7.0e-128	68.40	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GXQ0	2.8e-124	65.94	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1E0E4	1.4e-123	69.44	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of MS014493 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	1.2e-98	55.10	fatty acid desaturase 5	[more]
AT1G06080.1	3.5e-87	49.19	delta 9 desaturase 1	[more]
AT2G31360.1	8.0e-84	48.66	16:0delta9 desaturase 2	[more]
AT3G15870.1	7.5e-82	49.12	Fatty acid desaturase family protein	[more]
AT1G06100.1	1.2e-76	46.60	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 70..90 score: 32.83 coord: 107..136 score: 42.63
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 16..304
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 46..300 e-value: 3.30663E-63 score: 196.238
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 52..282 e-value: 3.3E-17 score: 63.2
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 16..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014493.1	MS014493.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water