MS014310 (gene) Bitter gourd (TR) v1

BLAST of MS014310 vs. NCBI nr
Match: XP_022131596.1 (subtilisin-like protease SBT1.7 [Momordica charantia])

HSP 1 Score: 1505.7 bits (3897), Expect = 0.0e+00
Identity = 764/767 (99.61%), Postives = 764/767 (99.61%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL
Sbjct: 7   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 66

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL
Sbjct: 67  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 126

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN
Sbjct: 127 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 186

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT
Sbjct: 187 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 246

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 247 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 306

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES
Sbjct: 307 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 366

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLPDSLVPIVYAAHASNSTSGS CLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV
Sbjct: 367 LYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 426

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR
Sbjct: 427 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 486

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI
Sbjct: 487 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 546

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF
Sbjct: 547 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 606

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNPGAALDPGLVYDTTTDDYF FLCALNYTSLQIKVIAKKDFTCSASKNYKLED
Sbjct: 607 DIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 666

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVS TSKSPSVKIVVEPES
Sbjct: 667 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPES 726

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT
Sbjct: 727 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 773

BLAST of MS014310 vs. NCBI nr
Match: XP_038885098.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1365.1 bits (3532), Expect = 0.0e+00
Identity = 683/767 (89.05%), Postives = 728/767 (94.92%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCR+S+ FLLFLI F S S+  A+K+N++LKKKTY+IHMDRTNMPQAFDDHF+WYDSSL
Sbjct: 4   QTCRLSQWFLLFLISFCSCSFTEAQKSNRQLKKKTYVIHMDRTNMPQAFDDHFKWYDSSL 63

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVS+SAQMLYSY+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KYELHTTRTPEFLGL
Sbjct: 64  KSVSNSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGL 123

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKS SFFPAS KV EVIIG+LDTGVWPELESFNDAGLGPVP SWKGECEVGKNF+SSSCN
Sbjct: 124 GKSVSFFPASEKVSEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCN 183

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A+GT
Sbjct: 184 RKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGT 243

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAA+ARVATYKVCWLGGCF SDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 244 ARGMAAEARVATYKVCWLGGCFSSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 303

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSAAAQGVFVSCSAGNGGPSSG+LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGES
Sbjct: 304 AFSAAAQGVFVSCSAGNGGPSSGTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGES 363

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLPDSL+PIVYA +ASNSTSGS+CL+ TLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 364 LYSGKPLPDSLLPIVYAGNASNSTSGSLCLSSTLNPAKVAGKIVVCDRGGNSRVQKGLVV 423

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGG GMILANTE YGEE+LADAHL P AAVG+K+GDAIKSYISSDANPTA  STGTTR
Sbjct: 424 KEAGGVGMILANTEAYGEEQLADAHLTPTAAVGKKSGDAIKSYISSDANPTATISTGTTR 483

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFNI
Sbjct: 484 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNI 543

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQD+S+G PSTPF
Sbjct: 544 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEVIQDVSNGSPSTPF 603

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYDTTTDDYFAFLCALNY+SLQIKVI+K+DFTC+ +KNYKLED
Sbjct: 604 DIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVISKRDFTCNGNKNYKLED 663

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPS RGG D APTTVKYTRTLTNK A STYKVSVT+    VKIVVEPES
Sbjct: 664 LNYPSFAVPLETPSTRGGEDVAPTTVKYTRTLTNKGAPSTYKVSVTAPK-EVKIVVEPES 723

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSFAE NEQKSYTVTFIASPMPSG+ESFARLEWSDGKHIVGSPIAFT
Sbjct: 724 LSFAEANEQKSYTVTFIASPMPSGSESFARLEWSDGKHIVGSPIAFT 769

BLAST of MS014310 vs. NCBI nr
Match: XP_004142884.1 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KGN62460.1 hypothetical protein Csa_018794 [Cucumis sativus])

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 676/767 (88.14%), Postives = 725/767 (94.52%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           +TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+TNMPQAFDDHFQWYDSSL
Sbjct: 2   KTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSSL 61

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSY+TVIHGFSTRLTVEEA+LMEK+EGI+AVIPE KYELHTTRTPEFLGL
Sbjct: 62  KSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLGL 121

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKS SFFPAS KV EVIIG+LDTGVWPELESF+DAGLGP+P SWKGECEVGKNF+SS+CN
Sbjct: 122 GKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNCN 181

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+GT
Sbjct: 182 RKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGT 241

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAA+ARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG+S DYYRDNVAIG
Sbjct: 242 ARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAIG 301

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGES
Sbjct: 302 AFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGES 361

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLP+SL+PIV AA ASNS+SGS+CL+GTLNPAKV GKIVVCDRGGNSRVQKGVVV
Sbjct: 362 LYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVVV 421

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIK+YISSD+NPTA  STGTTR
Sbjct: 422 KEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTTR 481

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFNI
Sbjct: 482 LGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNI 541

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTPF
Sbjct: 542 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTPF 601

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYDTTTDDY AFLCALNY+SLQIKVI+KKDFTC+ +KNYKLED
Sbjct: 602 DIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLED 661

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPS RGG + APTT+KYTRTLTNK ASSTYKVSVT+KS SVKIVVEPES
Sbjct: 662 LNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPES 721

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFT
Sbjct: 722 LSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFT 768

BLAST of MS014310 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 676/768 (88.02%), Postives = 724/768 (94.27%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYDSSL
Sbjct: 4   QTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 63

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEFLGL
Sbjct: 64  KSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGL 123

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
            KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSSSCN
Sbjct: 124 EKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCN 183

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A+GT
Sbjct: 184 RKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGT 243

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           A+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 244 AKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIG 303

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFTG+S
Sbjct: 304 AFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQS 363

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LY+GKPL DSL+PIVYAAHASNSTSGS+CLT TLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 364 LYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVV 423

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S GTTR
Sbjct: 424 KEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTR 483

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGW G  GPTGLD DKR VAFNI
Sbjct: 484 LGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNI 543

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPSTPF
Sbjct: 544 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPF 603

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYD T +DYFAFLCALNY+S QIKVI+KKDFTCS +KNYKLED
Sbjct: 604 DIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLED 663

Query: 661 LNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPE 720
           LNYPSFAV LETPS +GGG+  APTTVKYTRTLTNK A STYKVSVTSKSPSVKI+VEPE
Sbjct: 664 LNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPE 723

Query: 721 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           SLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFT
Sbjct: 724 SLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFT 771

BLAST of MS014310 vs. NCBI nr
Match: KAG6598344.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 676/768 (88.02%), Postives = 724/768 (94.27%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYDSSL
Sbjct: 4   QTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 63

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEFLGL
Sbjct: 64  KSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGL 123

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
            KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSSSCN
Sbjct: 124 EKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCN 183

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A+GT
Sbjct: 184 RKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGT 243

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           A+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 244 AKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIG 303

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFTG+S
Sbjct: 304 AFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQS 363

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LY+GKPL DSL+PIVYAAHASNSTSGS+CLT TLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 364 LYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVV 423

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S GTTR
Sbjct: 424 KEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTR 483

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGW G  GPTGLD DKR VAFNI
Sbjct: 484 LGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNI 543

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPSTPF
Sbjct: 544 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPF 603

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYD T +DYFAFLCALNY+S QIKVI+KKDFTCS +KNYKLED
Sbjct: 604 DIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSRNKNYKLED 663

Query: 661 LNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPE 720
           LNYPSFAV LETPS +GGG+  APTTVKYTRTLTNK A STYKVSVTSKSPSVKI+VEPE
Sbjct: 664 LNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPE 723

Query: 721 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           SLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFT
Sbjct: 724 SLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFT 771

BLAST of MS014310 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 1.1e-288
Identity = 493/758 (65.04%), Postives = 602/758 (79.42%), Query Frame = 0

Query: 12  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLY 71
           FL++   + +V +  ++Q     TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY
Sbjct: 12  FLLLCLGFCHVSSSSSDQ----GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLY 71

Query: 72  SYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGK-SDSFFPAS 131
           +Y   IHGFSTRLT EEA+ +  + G+++V+PE +YELHTTRTP FLGL + +   FP +
Sbjct: 72  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 131

Query: 132 VKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFA 191
               +V++G+LDTGVWPE +S++D G GP+P+SWKG CE G NF++S CNRKLIGAR+FA
Sbjct: 132 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 191

Query: 192 KGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARV 251
           +GYES  GPIDES+ES+SP+DDDGHGTHTS+TAAGS V GA+L GYASGTARGMA +ARV
Sbjct: 192 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 251

Query: 252 ATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVF 311
           A YKVCWLGGCF SDILAAIDKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  +G+ 
Sbjct: 252 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 311

Query: 312 VSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDS 371
           VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD 
Sbjct: 312 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDK 371

Query: 372 LVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMIL 431
           L+P +YA +ASN+T+G++C+TGTL P KV GKIV+CDRG N+RVQKG VVK AGG GMIL
Sbjct: 372 LLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 431

Query: 432 ANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVA 491
           ANT   GEE +ADAHLLPA  VG+K GD I+ Y+++D NPTA  S   T +GV+PSPVVA
Sbjct: 432 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 491

Query: 492 AFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPH 551
           AFSSRGPN +TP+ILKPDLIAPGVNILA WTGAAGPTGL  D RRV FNIISGTSMSCPH
Sbjct: 492 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 551

Query: 552 ISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPG 611
           +SGLAAL+K+ HP+WSPAAIRSALMTTAY TYK+G+ + DI++G PSTPFD GAGHV+P 
Sbjct: 552 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 611

Query: 612 AALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPL 671
            A +PGL+YD TT+DY  FLCALNYTS QI+ ++++++TC  SK+Y + DLNYPSFAV  
Sbjct: 612 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-- 671

Query: 672 ETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPESLSFAEENEQK 731
              +V G G       KYTRT+T+   + TY V VTS++  VKI VEP  L+F E NE+K
Sbjct: 672 ---NVDGVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 731

Query: 732 SYTVTF-IASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           SYTVTF + S  PSG+ SF  +EWSDGKH+VGSP+A +
Sbjct: 732 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of MS014310 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.9e-221
Identity = 417/740 (56.35%), Postives = 513/740 (69.32%), Query Frame = 0

Query: 33  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAE-L 92
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y+T  HGFS  L   EA+ L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 93  MEKREGILAVIPEAKYELHTTRTPEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELES 152
           +     IL +  +  Y LHTTRTPEFLGL               VIIG+LDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 153 FNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFAKGYESAF-GPIDESQESKSPK 212
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR F+KG++ A  G     +ES SP+
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 213 DDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAI 272
           D DGHGTHTSTTAAGSAV  A+  GYA+GTARGMA +ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 273 DKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 332
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 333 WITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSICL 392
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  +  NS+S ++CL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 393 TGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAA 452
            G+L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 453 AVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLI 512
           AVG+KTGD ++ Y+ SD+ PTA      T L V+PSPVVAAFSSRGPN +TP ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 513 APGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAI 572
            PGVNILAGW+ A GPTGLD D RR  FNI+SGTSMSCPHISGLA L+KAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 573 RSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFL 632
           +SALMTTAY        + D +    S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 633 CALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTR 692
           C+L+YT   I  I K+     + K      LNYPSF       SV  GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF-------SVLFGG---KRVVRYTR 686

Query: 693 TLTN-KAASSTYKVSVTSKSPSVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTE--S 752
            +TN  AASS YKV+V   +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 753 FARLEWSDGKHIVGSPIAFT 768
           F  + WS+ +H V SP+AF+
Sbjct: 747 FGSITWSNPQHEVRSPVAFS 750

BLAST of MS014310 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.4e-216
Identity = 403/774 (52.07%), Postives = 519/774 (67.05%), Query Frame = 0

Query: 11  LFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 70
           LF+I+  +  ++ AE T Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 71  ---DSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLG 130
              ++ ++LY+Y T  HG + +LT EEAE +E+ +G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 131 KSDS--FFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 190
           + +S   +   V   +V++G+LDTG+WPE ESFND G+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 191 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 250
           NRK++GAR F +GYE+A G IDE  E KSP+D DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 251 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 310
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 311 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 370
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA + +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 371 SLYSGKPL--PDSLVPIVY-AAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQK 430
           SLY G+ +   +   P+VY   +AS+    S CL G L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 431 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 490
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA    
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 491 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 550
             TR+G++PSPVVAAFSSRGPN L+  ILKPDL+APGVNILA WTG   P+ L  D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 551 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 610
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 611 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAK-KDFTCSASKN 670
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + +  Q+KV  K  + TC  +  
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 671 YKLEDLNYPSF-AVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSVTSKSPSVK 730
               +LNYP+  A+  E   V+         +   RT+TN     S+YKVSV S      
Sbjct: 672 KNPGNLNYPAISALFPENTHVK--------AMTLRRTVTNVGPHISSYKVSV-SPFKGAS 731

Query: 731 IVVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           + V+P++L+F  ++++ SYTVTF  +        F  L W    H V SP+  T
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIIT 775

BLAST of MS014310 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 3.4e-210
Identity = 398/780 (51.03%), Postives = 523/780 (67.05%), Query Frame = 0

Query: 5   VSRLFLLF--LIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKS 64
           +S +F +F  L+ F+S S   ++        ++YI+H+ R++ P  F  H  W+ S L+S
Sbjct: 6   LSSIFFVFPLLLCFFSPSSSSSDGL------ESYIVHVQRSHKPSLFSSHNNWHVSLLRS 65

Query: 65  VSDSAQ---MLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 124
           +  S Q   +LYSYS  +HGFS RL+  +   + +   +++VIP+   E+HTT TP FLG
Sbjct: 66  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 125

Query: 125 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 184
             ++   +  S    +VI+G+LDTG+WPE  SF+D+GLGP+P++WKGECE+G +F +SSC
Sbjct: 126 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 185

Query: 185 NRKLIGARYFAKGYESAFGPIDE--SQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 244
           NRKLIGAR F +GY +      +  ++ES+SP+D +GHGTHT++TAAGS V  A+L+ YA
Sbjct: 186 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 245

Query: 245 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSPDYYRD 304
            GTA GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P+Y+ D
Sbjct: 246 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 305

Query: 305 NVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKK 364
           ++AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK 
Sbjct: 306 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 365

Query: 365 FTGESLYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQ 424
           FTG SLY+G+ LPDS + +VY    S      +C  G LN + V GKIV+CDRGGN+RV+
Sbjct: 366 FTGTSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVE 425

Query: 425 KGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFS 484
           KG  VK AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI +  +PTAK S
Sbjct: 426 KGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKIS 485

Query: 485 TGTTRLGVQ-PSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 544
              T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT LD D R
Sbjct: 486 FLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR 545

Query: 545 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 604
           RV FNIISGTSMSCPH+SGLAAL++ AHPDWSPAAI+SAL+TTAY    +GE I+D+++G
Sbjct: 546 RVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATG 605

Query: 605 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDF---TCS 664
             S  F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I V  +       C 
Sbjct: 606 KSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACE 665

Query: 665 ASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSP- 724
            SK     DLNYPSF+V   +             VKY R + N  ++      V  KSP 
Sbjct: 666 TSKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPA 725

Query: 725 SVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTES-----FARLEWSDGKHIVGSPIA 766
           +V+I V P  L+F++E     Y VTF +  +  G  S     F  +EW+DG+H+V SP+A
Sbjct: 726 NVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVA 766

BLAST of MS014310 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 7.9e-199
Identity = 379/775 (48.90%), Postives = 501/775 (64.65%), Query Frame = 0

Query: 12  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDS-AQML 71
           F   F+  +      +       TYI+H+D    P  F  HF WY SSL S++ S   ++
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSII 63

Query: 72  YSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGKSD--SFFP 131
           ++Y TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +D      
Sbjct: 64  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 123

Query: 132 ASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARY 191
            S    +++IG++DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+GAR+
Sbjct: 124 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 183

Query: 192 FAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQA 251
           F  GYE+  G ++E+ E +SP+D DGHGTHT++ +AG  V  A+  GYA G A GMA +A
Sbjct: 184 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 243

Query: 252 RVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQG 311
           R+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A  +G
Sbjct: 244 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRG 303

Query: 312 VFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPL- 371
           +FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA + LGNGK  +G S+Y G  L 
Sbjct: 304 IFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLD 363

Query: 372 PDSLVPIVYAAH--ASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGG 431
           P  + P+VY       +  S S+CL G+L+P  V GKIV+CDRG NSR  KG +V++ GG
Sbjct: 364 PGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGG 423

Query: 432 AGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYIS------SDANPTAKFSTGTT 491
            GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTA      T
Sbjct: 424 LGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGT 483

Query: 492 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 551
           RLG++P+PVVA+FS+RGPN  TP ILKPD+IAPG+NILA W    GP+G+  D RR  FN
Sbjct: 484 RLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFN 543

Query: 552 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 611
           I+SGTSM+CPH+SGLAAL+KAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G  S+ 
Sbjct: 544 ILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSV 603

Query: 612 FDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNY-KL 671
            D G+GHV+P  A+DPGLVYD T+ DY  FLC  NYT   I  I ++   C  ++    +
Sbjct: 604 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 663

Query: 672 EDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSVTSKSPSVKIVVE 731
            +LNYPSF+V  +       G++  +T  + RT+TN   S S Y++ +        + VE
Sbjct: 664 GNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPR-GTTVTVE 723

Query: 732 PESLSFAEENEQKSY-----TVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           PE LSF    ++ S+     T     SP  +  E+   + WSDGK  V SP+  T
Sbjct: 724 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVVT 770

BLAST of MS014310 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1505.7 bits (3897), Expect = 0.0e+00
Identity = 764/767 (99.61%), Postives = 764/767 (99.61%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL
Sbjct: 7   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 66

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL
Sbjct: 67  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 126

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN
Sbjct: 127 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 186

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT
Sbjct: 187 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 246

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 247 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 306

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES
Sbjct: 307 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 366

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLPDSLVPIVYAAHASNSTSGS CLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV
Sbjct: 367 LYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 426

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR
Sbjct: 427 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 486

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI
Sbjct: 487 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 546

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF
Sbjct: 547 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 606

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNPGAALDPGLVYDTTTDDYF FLCALNYTSLQIKVIAKKDFTCSASKNYKLED
Sbjct: 607 DIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 666

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVS TSKSPSVKIVVEPES
Sbjct: 667 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPES 726

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT
Sbjct: 727 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 773

BLAST of MS014310 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 676/767 (88.14%), Postives = 725/767 (94.52%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           +TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+TNMPQAFDDHFQWYDSSL
Sbjct: 2   KTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSSL 61

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSY+TVIHGFSTRLTVEEA+LMEK+EGI+AVIPE KYELHTTRTPEFLGL
Sbjct: 62  KSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLGL 121

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKS SFFPAS KV EVIIG+LDTGVWPELESF+DAGLGP+P SWKGECEVGKNF+SS+CN
Sbjct: 122 GKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNCN 181

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+GT
Sbjct: 182 RKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGT 241

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAA+ARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG+S DYYRDNVAIG
Sbjct: 242 ARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAIG 301

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGES
Sbjct: 302 AFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGES 361

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLP+SL+PIV AA ASNS+SGS+CL+GTLNPAKV GKIVVCDRGGNSRVQKGVVV
Sbjct: 362 LYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVVV 421

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIK+YISSD+NPTA  STGTTR
Sbjct: 422 KEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTTR 481

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFNI
Sbjct: 482 LGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNI 541

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTPF
Sbjct: 542 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTPF 601

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYDTTTDDY AFLCALNY+SLQIKVI+KKDFTC+ +KNYKLED
Sbjct: 602 DIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLED 661

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPS RGG + APTT+KYTRTLTNK ASSTYKVSVT+KS SVKIVVEPES
Sbjct: 662 LNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPES 721

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFT
Sbjct: 722 LSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFT 768

BLAST of MS014310 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1342.0 bits (3472), Expect = 0.0e+00
Identity = 672/768 (87.50%), Postives = 722/768 (94.01%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYDSSL
Sbjct: 4   QTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 63

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEFLGL
Sbjct: 64  KSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGL 123

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
            KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSSSCN
Sbjct: 124 EKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCN 183

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+ +GT
Sbjct: 184 RKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFGAGT 243

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           A+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNVAIG
Sbjct: 244 AKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIG 303

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFTG+S
Sbjct: 304 AFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQS 363

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LY+GKPL DSL+PIVYAAHASNSTSGS+CLT TLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 364 LYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVV 423

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           K+AGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S G+TR
Sbjct: 424 KDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGSTR 483

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGWT   GPTGLD DKR VAFNI
Sbjct: 484 LGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVAFNI 543

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPSTPF
Sbjct: 544 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPF 603

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYD T +DYFAFLCALNY+S QIKVI+KKDFTCS +KNYKLED
Sbjct: 604 DIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLED 663

Query: 661 LNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPE 720
           LNYPSFAV LETPS +GG +  APTTVKYTRTLTNK A STYKVSVTSKSPSVKI+VEPE
Sbjct: 664 LNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPE 723

Query: 721 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           SLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFT
Sbjct: 724 SLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFT 771

BLAST of MS014310 vs. ExPASy TrEMBL
Match: A0A1S3BA54 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=1)

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 668/767 (87.09%), Postives = 720/767 (93.87%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           +TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+T+MPQAFDDHFQWYD+SL
Sbjct: 2   KTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTSL 61

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSY+ VIHGFST LTVEEA+LMEK+EGI+AV+PE KYELHTTRTPEFLGL
Sbjct: 62  KSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLGL 121

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKS SFFPAS KV EVIIGILDTGVWPELESF+D GLGP+P SWKGECEVGKNF+SSSCN
Sbjct: 122 GKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSCN 181

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+GT
Sbjct: 182 RKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGT 241

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAA+ARVATYKVCWLGGCF SDILAAIDK+VEDG N++S+SLGG+S DYYRDNVAIG
Sbjct: 242 ARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAIG 301

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGES
Sbjct: 302 AFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGES 361

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLP+SL+PIV AA ASNS+SGS+CL+GTLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 362 LYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLVV 421

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGG GMILANTE YGEE+LADAHL+P AAVGQ+ GDAIKSYISSD+NPTA  STGTTR
Sbjct: 422 KEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTTR 481

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFNI
Sbjct: 482 LGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNI 541

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTPF
Sbjct: 542 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPF 601

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYDTTTDDY AFLCALNY+SLQIKVI+KKDFTC+ +KNYKLED
Sbjct: 602 DIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLED 661

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPS + G + APTTVKYTRTLTNK A STYKVSVT+K  SVKIVV PES
Sbjct: 662 LNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAPES 721

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFT
Sbjct: 722 LSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFT 768

BLAST of MS014310 vs. ExPASy TrEMBL
Match: A0A5A7UYG7 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00750 PE=3 SV=1)

HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 667/767 (86.96%), Postives = 718/767 (93.61%), Query Frame = 0

Query: 1   QTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSL 60
           +TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+T+MPQAFDDHFQWYD+ L
Sbjct: 2   KTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTCL 61

Query: 61  KSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGL 120
           KSVSDSAQMLYSY+ VIHGFST LTVEEA+LMEK+EGI+AV+PE KYELHTTRTPEFLGL
Sbjct: 62  KSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLGL 121

Query: 121 GKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCN 180
           GKS SFFPAS KV EVIIGILDTGVWPELESF+D GLGP+P SWKGECEVGKNF+SSSCN
Sbjct: 122 GKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSCN 181

Query: 181 RKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGT 240
           RKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+GT
Sbjct: 182 RKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGT 241

Query: 241 ARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIG 300
           ARGMAA+ARVATYKVCWLGGCF SDILAAIDK+VEDG N++S+SLGG+S DYYRDNVAIG
Sbjct: 242 ARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAIG 301

Query: 301 AFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGES 360
           AFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGES
Sbjct: 302 AFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGES 361

Query: 361 LYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVV 420
           LYSGKPLP+SL+PIV AA ASNS+SGS+CL+GTLNPAKVAGKIVVCDRGGNSRVQKG+VV
Sbjct: 362 LYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLVV 421

Query: 421 KEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTR 480
           KEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIKSYISSD+NPTA  STGTTR
Sbjct: 422 KEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKSYISSDSNPTATISTGTTR 481

Query: 481 LGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNI 540
           LGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFNI
Sbjct: 482 LGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNI 541

Query: 541 ISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPF 600
           ISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTPF
Sbjct: 542 ISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPF 601

Query: 601 DIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLED 660
           DIGAGHVNP AALDPGLVYDTTTDDY AFLCALNY+SLQIKVI+KKDFTC+ +KNYKLED
Sbjct: 602 DIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLED 661

Query: 661 LNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPES 720
           LNYPSFAVPLETPS + G + APTTVKYTRTLTNK   STYKVSVT+K  SVKIVV PES
Sbjct: 662 LNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGVPSTYKVSVTAKISSVKIVVAPES 721

Query: 721 LSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           LSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFT
Sbjct: 722 LSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFT 768

BLAST of MS014310 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 993.8 bits (2568), Expect = 7.6e-290
Identity = 493/758 (65.04%), Postives = 602/758 (79.42%), Query Frame = 0

Query: 12  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLY 71
           FL++   + +V +  ++Q     TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY
Sbjct: 12  FLLLCLGFCHVSSSSSDQ----GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLY 71

Query: 72  SYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGK-SDSFFPAS 131
           +Y   IHGFSTRLT EEA+ +  + G+++V+PE +YELHTTRTP FLGL + +   FP +
Sbjct: 72  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 131

Query: 132 VKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFA 191
               +V++G+LDTGVWPE +S++D G GP+P+SWKG CE G NF++S CNRKLIGAR+FA
Sbjct: 132 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 191

Query: 192 KGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARV 251
           +GYES  GPIDES+ES+SP+DDDGHGTHTS+TAAGS V GA+L GYASGTARGMA +ARV
Sbjct: 192 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 251

Query: 252 ATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVF 311
           A YKVCWLGGCF SDILAAIDKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  +G+ 
Sbjct: 252 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 311

Query: 312 VSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDS 371
           VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD 
Sbjct: 312 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDK 371

Query: 372 LVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMIL 431
           L+P +YA +ASN+T+G++C+TGTL P KV GKIV+CDRG N+RVQKG VVK AGG GMIL
Sbjct: 372 LLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 431

Query: 432 ANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVA 491
           ANT   GEE +ADAHLLPA  VG+K GD I+ Y+++D NPTA  S   T +GV+PSPVVA
Sbjct: 432 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 491

Query: 492 AFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPH 551
           AFSSRGPN +TP+ILKPDLIAPGVNILA WTGAAGPTGL  D RRV FNIISGTSMSCPH
Sbjct: 492 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 551

Query: 552 ISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPG 611
           +SGLAAL+K+ HP+WSPAAIRSALMTTAY TYK+G+ + DI++G PSTPFD GAGHV+P 
Sbjct: 552 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 611

Query: 612 AALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPL 671
            A +PGL+YD TT+DY  FLCALNYTS QI+ ++++++TC  SK+Y + DLNYPSFAV  
Sbjct: 612 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-- 671

Query: 672 ETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSPSVKIVVEPESLSFAEENEQK 731
              +V G G       KYTRT+T+   + TY V VTS++  VKI VEP  L+F E NE+K
Sbjct: 672 ---NVDGVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 731

Query: 732 SYTVTF-IASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           SYTVTF + S  PSG+ SF  +EWSDGKH+VGSP+A +
Sbjct: 732 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of MS014310 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 770.4 bits (1988), Expect = 1.4e-222
Identity = 417/740 (56.35%), Postives = 513/740 (69.32%), Query Frame = 0

Query: 33  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAE-L 92
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y+T  HGFS  L   EA+ L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 93  MEKREGILAVIPEAKYELHTTRTPEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELES 152
           +     IL +  +  Y LHTTRTPEFLGL               VIIG+LDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 153 FNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFAKGYESAF-GPIDESQESKSPK 212
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR F+KG++ A  G     +ES SP+
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 213 DDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAI 272
           D DGHGTHTSTTAAGSAV  A+  GYA+GTARGMA +ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 273 DKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 332
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 333 WITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSICL 392
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  +  NS+S ++CL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 393 TGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAA 452
            G+L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 453 AVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLI 512
           AVG+KTGD ++ Y+ SD+ PTA      T L V+PSPVVAAFSSRGPN +TP ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 513 APGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAI 572
            PGVNILAGW+ A GPTGLD D RR  FNI+SGTSMSCPHISGLA L+KAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 573 RSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFL 632
           +SALMTTAY        + D +    S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 633 CALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTR 692
           C+L+YT   I  I K+     + K      LNYPSF       SV  GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF-------SVLFGG---KRVVRYTR 686

Query: 693 TLTN-KAASSTYKVSVTSKSPSVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTE--S 752
            +TN  AASS YKV+V   +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 753 FARLEWSDGKHIVGSPIAFT 768
           F  + WS+ +H V SP+AF+
Sbjct: 747 FGSITWSNPQHEVRSPVAFS 750

BLAST of MS014310 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 754.2 bits (1946), Expect = 1.0e-217
Identity = 403/774 (52.07%), Postives = 519/774 (67.05%), Query Frame = 0

Query: 11  LFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 70
           LF+I+  +  ++ AE T Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 71  ---DSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLG 130
              ++ ++LY+Y T  HG + +LT EEAE +E+ +G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 131 KSDS--FFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 190
           + +S   +   V   +V++G+LDTG+WPE ESFND G+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 191 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 250
           NRK++GAR F +GYE+A G IDE  E KSP+D DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 251 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 310
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 311 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 370
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA + +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 371 SLYSGKPL--PDSLVPIVY-AAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQK 430
           SLY G+ +   +   P+VY   +AS+    S CL G L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 431 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 490
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA    
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 491 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 550
             TR+G++PSPVVAAFSSRGPN L+  ILKPDL+APGVNILA WTG   P+ L  D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 551 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 610
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 611 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAK-KDFTCSASKN 670
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + +  Q+KV  K  + TC  +  
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 671 YKLEDLNYPSF-AVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSVTSKSPSVK 730
               +LNYP+  A+  E   V+         +   RT+TN     S+YKVSV S      
Sbjct: 672 KNPGNLNYPAISALFPENTHVK--------AMTLRRTVTNVGPHISSYKVSV-SPFKGAS 731

Query: 731 IVVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           + V+P++L+F  ++++ SYTVTF  +        F  L W    H V SP+  T
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIIT 775

BLAST of MS014310 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 733.0 bits (1891), Expect = 2.4e-211
Identity = 398/780 (51.03%), Postives = 523/780 (67.05%), Query Frame = 0

Query: 5   VSRLFLLF--LIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKS 64
           +S +F +F  L+ F+S S   ++        ++YI+H+ R++ P  F  H  W+ S L+S
Sbjct: 6   LSSIFFVFPLLLCFFSPSSSSSDGL------ESYIVHVQRSHKPSLFSSHNNWHVSLLRS 65

Query: 65  VSDSAQ---MLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 124
           +  S Q   +LYSYS  +HGFS RL+  +   + +   +++VIP+   E+HTT TP FLG
Sbjct: 66  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 125

Query: 125 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 184
             ++   +  S    +VI+G+LDTG+WPE  SF+D+GLGP+P++WKGECE+G +F +SSC
Sbjct: 126 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 185

Query: 185 NRKLIGARYFAKGYESAFGPIDE--SQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 244
           NRKLIGAR F +GY +      +  ++ES+SP+D +GHGTHT++TAAGS V  A+L+ YA
Sbjct: 186 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 245

Query: 245 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSPDYYRD 304
            GTA GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P+Y+ D
Sbjct: 246 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 305

Query: 305 NVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKK 364
           ++AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK 
Sbjct: 306 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 365

Query: 365 FTGESLYSGKPLPDSLVPIVYAAHASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQ 424
           FTG SLY+G+ LPDS + +VY    S      +C  G LN + V GKIV+CDRGGN+RV+
Sbjct: 366 FTGTSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVE 425

Query: 425 KGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFS 484
           KG  VK AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI +  +PTAK S
Sbjct: 426 KGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKIS 485

Query: 485 TGTTRLGVQ-PSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 544
              T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT LD D R
Sbjct: 486 FLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR 545

Query: 545 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 604
           RV FNIISGTSMSCPH+SGLAAL++ AHPDWSPAAI+SAL+TTAY    +GE I+D+++G
Sbjct: 546 RVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATG 605

Query: 605 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDF---TCS 664
             S  F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I V  +       C 
Sbjct: 606 KSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACE 665

Query: 665 ASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSVTSKSP- 724
            SK     DLNYPSF+V   +             VKY R + N  ++      V  KSP 
Sbjct: 666 TSKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPA 725

Query: 725 SVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTES-----FARLEWSDGKHIVGSPIA 766
           +V+I V P  L+F++E     Y VTF +  +  G  S     F  +EW+DG+H+V SP+A
Sbjct: 726 NVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVA 766

BLAST of MS014310 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 695.3 bits (1793), Expect = 5.6e-200
Identity = 379/775 (48.90%), Postives = 501/775 (64.65%), Query Frame = 0

Query: 12  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDS-AQML 71
           F   F+  +      +       TYI+H+D    P  F  HF WY SSL S++ S   ++
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSII 63

Query: 72  YSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGKSD--SFFP 131
           ++Y TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +D      
Sbjct: 64  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 123

Query: 132 ASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARY 191
            S    +++IG++DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+GAR+
Sbjct: 124 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 183

Query: 192 FAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQA 251
           F  GYE+  G ++E+ E +SP+D DGHGTHT++ +AG  V  A+  GYA G A GMA +A
Sbjct: 184 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 243

Query: 252 RVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQG 311
           R+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A  +G
Sbjct: 244 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRG 303

Query: 312 VFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPL- 371
           +FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA + LGNGK  +G S+Y G  L 
Sbjct: 304 IFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLD 363

Query: 372 PDSLVPIVYAAH--ASNSTSGSICLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGG 431
           P  + P+VY       +  S S+CL G+L+P  V GKIV+CDRG NSR  KG +V++ GG
Sbjct: 364 PGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGG 423

Query: 432 AGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYIS------SDANPTAKFSTGTT 491
            GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTA      T
Sbjct: 424 LGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGT 483

Query: 492 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 551
           RLG++P+PVVA+FS+RGPN  TP ILKPD+IAPG+NILA W    GP+G+  D RR  FN
Sbjct: 484 RLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFN 543

Query: 552 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 611
           I+SGTSM+CPH+SGLAAL+KAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G  S+ 
Sbjct: 544 ILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSV 603

Query: 612 FDIGAGHVNPGAALDPGLVYDTTTDDYFAFLCALNYTSLQIKVIAKKDFTCSASKNY-KL 671
            D G+GHV+P  A+DPGLVYD T+ DY  FLC  NYT   I  I ++   C  ++    +
Sbjct: 604 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 663

Query: 672 EDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSVTSKSPSVKIVVE 731
            +LNYPSF+V  +       G++  +T  + RT+TN   S S Y++ +        + VE
Sbjct: 664 GNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPR-GTTVTVE 723

Query: 732 PESLSFAEENEQKSY-----TVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 768
           PE LSF    ++ S+     T     SP  +  E+   + WSDGK  V SP+  T
Sbjct: 724 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVVT 770