MS014232 (gene) Bitter gourd (TR) v1

Overview


Name	MS014232
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Neutral ceramidase
Location	scaffold5: 1538742 .. 1545333 (-)
RNA-Seq Expression	MS014232
Synteny	MS014232

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTTCCTTCTCTGTTTAATTTCATCATTCGGAGACCGTGTGAAGCTCTGTTTTGGACTTGTGCATTACTACTGCTGCAGAATGATAAAGGCTTCTCGTCGGACTCCAACTATTTGATCGGGGTTGGAAGCTACGACATCACTGGGCCGGCGGCGGATGTCAACATGATGGGATATGCCAACATGGAGCAGATTGCATCAGGAGTTCACTTCAGATTGCGAGCTCGTACATTCATTGTCGCGGAGCCACAAGGAGAAAGGGTTGTATTCGTTAATCTCGATGCTTGCATGGCCTCGCAGCTGGTGACGATCAAGGTGCTCCAGAGATTGAAGGCAAGGTATTTGAAACATTGCATCTCAACCTAAAACTATGGCTCTGTAAGTTAAGAGGATTGCTAGAAATCTATATGTATTATTGATGTTTATTTCGTTATGAAATTTGGTAATGGGGGATGGAGAAGTCGTTTGGACTTTGGAGCCAGCTTGTAATGTCGTTCATTTTCAGGTATGGAAACCTCTACACTGAAAAAAATGTCGCCATTAGTGGAATCCATACTCATGCTGGACCAGGTGGTTATCTCCAGTACATTGTATACATAGTGACATCTCTTGGATTTGTGCGACAGTCATTTGATGCCCTTGTAAATGGTATTGAGAACAGCATTATAGAGGCTCATGAGAATCTGCGCCCAGGATCAATTTTCATAAACAAGGGTAAGGGATAGGGCTCATGCTCATTGCACTTTATTAAGTGGATGTGCTGCATTTTTCTCTTTCTCTTCCTTAGTGATTTGATATTACTGCAAAGAGATATGTATGTTCTCAAGATGGCATAGACACAGATTCTGTGAGTATCGTGTGGAATAGAATGCTTTTGTTTGTATCTAACAATATGAGATTTCATCTGATGACTGCATGAAGCAACTCATTGTAGATTGGATCACTTTTTGGAGTATTGAAAGATGAATTGCCTACGTTTTTGGTTTCTCAACTTGCAGGAAAGCTGTTAGATGCAGGTGTAAACCGCAGCCCTAGTGCATATCTCAATAACCCTGGAGAAGAGCGTCGTAAATACAAATACAATGTTGACAAGCAGATGACACTTCTAAAATTTGTGGATCAGGAGTGGGGCCCTATTGGTAGCTTTAATTGGTTTTCGACTCATGGAACTTCTATGAGTCGTACAAACTCGTTAATTAGTGGGGACAACAAAGGTGCTGCTGCACGATTCATGGAGGACTGGTTTGAGAAAAAAGGAGGTATAAACTTGGATTTGGATATATCTGTAGATGACAGAATCCCACGAAGAGTCTCAAACATAATACCAGACCTGTCCATTAATTGTAAGTTATTTTCACAAGCTTTACGTCAAAGTGCTGTTTAATCAATTCAACTTTTGGAAACTGATAAGCTGGGACAGGTAATGTGGATGAATGTATAGTATGGTTACAGCTATAGTTCTGTATTTGCTATAGCTGCTTGTTCATTAGTCTGTACCAAATGGTCTGATATCTCTACATACCAACTCCTCAGTAATTCCTTTATCAATGTTGATAGGGCCAGAAAATCTGTAAATGTTTGAACAGCCATGTTTCTGATCAATCAATTCTTCTGGCTATATTATAATTTATGTTAGGTAATATGTTTTTAAGTTGTTTCTAAAATCAGAAGATAGGAAAGGGACGATATTTTCTCTCCGATACTTAATATGGGACCGGTCCCAGTTGTCATCCTCTCACCAACTTTAACCTCACGTACGGGATTGGGTCTACTCTCTTGCCTGAATTTATAGTTCTAGTAAAGATTACCTTAACCTAGCTTCCCATACCAATACCATTATTCCTTTCTATATCTCAACCAGTAAATTAAAAAGATGTTCACCAATTGATAACCATAGAAAAATCTTAACCACCTTAAAACAAGATCCAATGAATTGTTATACTTTCATAGCAAGCACCTACTTGACTTGTCAATTTTGTTCGCTCCTCTTTTGGTTAAGATGTGTGCCAAAAGTAAATTATTGGTGCGACTGTGACATATTTGGTAGGGTACTCAATGATTTACATGATACGATGGGCTTCTCATGGTATATTATAACTAGTGGATTCATCCAGTGTGTCTATCCTTACTCCTTAGATCCTATCAGGTACTGACCAGTAGAGTAGAGTAAATTTTAACTGCAGAAGGCGTAAATACGTTTTAAAATGTCTAATATGCATCGTGCATCTGGTTTTGATTATGAATACTCTTTGAAGCTGCATAAGTGTAGCAGATAACTGTAAAAGTCAACAAATAAATAAAAAAGAATGTTCTTCTGTAGATAGGAAACATATCATTCTCAATTTCCTGGTATCTTTCTGACACAGATCATGAGTTACTTGAGCTTGCTGTATCATTCCAATCTCCTTCCGGTAGATCAGCAACCAGAATTCTGAGTTCTTCAAAGCGTGTCAGGGGTGCCCTCAGGAATGCCGACAAACCCCGATTTGTGTCTGCATTTTGTCAATCAAACTGCGGTGATGTTAGCCCAAATGTTCTTGGAACATTCTGTATAGATACTGGACTTCCATGTGATTTCAATCACAGTACCTGTGATGGGAAGAATGAGCTATGCTACGGCAGAGGACCAGGGTAAGCGTCCTGCATTGAAGAACCTTATACAATTTCAGAGAAGCTATAAATAAATTGTGATGTCAATATGGCAGTTATCCAGATGAATTCGAAAGCACGCGGATTATAGGAAAGAGACAATTCAGAAAAGCAGTTGATCTATTTAGTCATGCATCAGAGCAGTTAACAGGAAAGGTTGATTACCGTCACACATATGTAGACTTCTCTCAGCTTGAGGTAACTCTTGAACGTGGAGGAACTACTGAAGTGGTACAAACATGTCCTGCTGCAATGGGTTTCGCATTTGCCGCTGGAACCACTGATGGACCAGGTGCTTTTGATTTTGAGCAAGGAGATGACAAGGTAATGATGATATTGGTTGGTAGTTTTTACGATATGGATGCTTTTATCCAATCTTATAAACTCTTTTACCATCCTTTTATACCTCAATGATGTTAATTTATCAGGGAAATCCTTTTTGGAGGTTCATACGCAATCTTTTGAAGAAACCAGGGAAGGAACAAGTTGATTGTCAACAGCCAAAGCCCATCTTGCTGGATACTGGTGAAATGAGAAAACCATATGAGTGGGCTGTAAGTTCCAATCAATAAACTTTTCTTAGCAATGATAAATTTTTTTGCTTCTTTATATCATATCTGTTTAAAATCTGCAAATACTATTTACTTGTGAAGTATAGTAATTTTGTTGTTAATATAGATATAACTCCCATAGTAAAGTAATCACATTCATGAAAATTAGAGGTTCATAATGTAGGTCAAAATCCTGCTTTGGTCCTGCTTCAGAGACTTTGTGTGGGATTTGGTTGGAGAGAAACAACATAATCTTTAGTGAGTGGAGAGGGATAGGGAGTCTATTTGGGATCTCATTAGATTTAATGTCTCTTAATGGGAGTTTGTTTCGAATTTGTTTTGTAATTATCAGTTAAGTTTTATTCTTTTGGATTGGAAGCCCTTTCTTTAGGTATTCCTCCGGCTTTTGTTTTTGTATGCCCTTTATATGTGTTCCTATTTTCATTTGTCCCAATGAAAAGTTCGGTTTCTTATAAAATAAAATAAAAAGAAATTTAATATCTAACTTACTACATACATGTAGATTCATACCTGTTCATATAGACATCTAATCATGTGGTCAACAAACAATAAATAATTTTAAGAAAATAACGATAAGGACTAAAATGAGTTTTATGCAAAGTCCAAAGAGTAAATAGAATATTCAAAAGTCTAGGGACTAAAATGGAATAAATATGAGAATTCAAAACTAAAATCTGTATCATAACTCTTTCAAATTAGATATGGGCCATATGGTCACTTTAAATTTCTTTGCAAGGATGCTGGTAACACTGTAATCAATAATTGTGATTACAAAAAAGAATTGACACAAATCTCTGGGTTCAGTAGTTTATACTCTTTTTCAACAGAAGCACAATTTTCTTGGTTAATTCAAATAATCAGTCCACGTCTTTCAGTATTAGATTTACAATCGTAGTCAGGATTTCTTTATATTTAAGAAGTAGTGAATGCATGAAAATTATAAACATGCGACAAAATACTACAATATTAAACTGAGGGATGCTCACCAATAAAATATGACTAATGAAACCGCATCTGAATTCCTCTGATCATGGAACATCTGGGTCAATACTTATCCAAATCCACGTATTCTGAGAATTTATTTTTCATTGTTTCTACAGCCTTCAATACTTCCCATCCAAATCCTCAGAGTAGGGCAGCTAATCATTCTTGGAGTACCTGGAGGTTGTAGGTTTCTTTATCCTACTTTTTTTTTACCAGTCTATACATTTTGTTCTGATATTGAGAAACTCACCTTCATATTCGACCTCCATTTTTGTTATAAAACTGTACATGCTGCTTTCAAATCTCAATATTTGCACCTGCAGAAATTGCTTGTTGAAGTTGTTAGTTTTTCCTACAAATGAGTTTGTATGCGATTCTTGTGCAAACCAAAAGCTTGGGTGGACCAAGTAGGTCAAATTATGCATATATTATTATGTCGGAAGTGCAAGTGGTACAAGTTACAAACTAATTGGCTAAATTATGTCTGAAGTGCAAGTGGTACAAGTTACAAACTAATTGGCTTGATTTGGAGGAGGTGAATGTGGGGAATATTTTGAGCTTTAATTTCTTTATCATTATCAATGGAAATAGAAGGCCCCAGGGAAGACCGTCCATGTCCTGACAGGAAGGATCTCTCTCATCTTCTAACCTATCTTCAATTTGTGGATGATACTCCCTTGTTTACATCTATGGATTGGGAAAAGTTCTCGATCTTCTTCAGCTCATAAATTATTTGAGAAAGCCTACGTTTATCAGTGAATTTAGCTTAATTGGATATCTATGCTGTTAACGTTCCTCTTCTTTTTTATTTTTTCTGTTTCTTTGGATTTTATTTTGGAGGGAATCCAAGGAGTTGAAGTTCTGAAACCTGGTTGTGGAGAGGTTCATGAGAAAATTTAGGTGGAAAAGAGCCCCTATTTCCCACTCTTGAACAATCCAGCCTTAGGAACCCAAAAGGAATTTCCCAAGGGTCTTTTTTTTCTTAAAAAAAATACTTTCCTATTTTTTAGAAGGGTGTTTGAAATTAAAAAAAAGAGGAATAATGGTAAAGCAAACTATAGATCCAGTTGAACAAATTCATGGTTCTTGGGGTAATAGGAGACAGTTAATAGGTTGATATTCATTTTATGTTTAAGTTTGCTTTCTATTAGCTTCATGTCTACCTTCATTGTATAAAAGGTTCTTGTTATATTTTCCCAGAAATTATAATTCCATAGTCACTTTATTACATCCATCCTGAACTGCTGGACTTCTTTTCCAACCAGAATTCACAACAATGGCTGGAAGGCGCCTTCGTGATGCTGTGAAGACCGTGCTTACCGATGGAGATAAAAATGGATTAAGTGGCGACATTCATATTGTTATTGCAGGACTAACAAATTCTTATTCACAGTATGTCACCACTTTCGAAGAGTACCAGGTGCAGAGATATGAGGTAAGATTTATATGTAGAGAGTCGTGTGTGAAATTGATTTAGAGGCGATTTAGGAGATTCTCTCATAGTGAGAAGTATTTAGCCACAGGAAAAGAAAATTAGTAAGGTGAAACAGGGATAAAATTCAACCCAATGGTATTGGTTACAGATAATGAGGATTATCAGAAAAAATTACATTTCCTTGTCGAAAAACCTGAAACTTGTAAACGCTGTAATCTTGATATTGCTATTTGGAATTCTGCATGAACACATCTTATTGTTCATCTTATTATTGAGGTATTATTGGATTCTTAATTTTCTCTCCTAGCTGCTAGCTCTGAGAACGCATAAAAGCAGTGTAGCGCAGCCAACTGCCAAGAACTTCGCATTTTCCGACCTATCAATGTAAGATCTTTGTTTTCAGGGCGCTTCTACGTTATACGGTCCTCACACTCTCAGTGCCTACATTCAGGAGTTTAAGAAGCTTGCCTGTGCACTCATAGATGGCCGAGAAGTTGGCGCAGGGCCACAACCTCCAGATTTACTAGACAAGCAGATAGGCTTACTACCACCTGTTGTGATGGATACGACTCCTACTTCAATAAAATTTGGGGATATAAGATCTGATGTTCCCCAGAATTCCACCTTCAACAGAGGCGATATGGTGACGGTTACCTTCTGGTCAGCCTGTCCTCGGAACGATCTTATGACTGAAGGTACGTTTGCCCTGGTGGAGATTCTCCACAGCAAAAAGCTGTGGGTTCCAGCCTACGATGATGATGATTTCTGCCTGCGTTTCAAGTGGTCGAGACCGTTCAAACTTAGCAGTCGAAGTCATGCAACCATAGAATGGAGAATCCCTAAAACTGCAATTCCAGGAATGTACAGAATAACACACTTTGGGGCAGCAAAAAGTCTTTTCGGATCAATCCACCATTTCACAGGCTCGTCTAGTCCTTTTGGTGTAGCG

mRNA sequence

ATGGAAGTTCCTTCTCTGTTTAATTTCATCATTCGGAGACCGTGTGAAGCTCTGTTTTGGACTTGTGCATTACTACTGCTGCAGAATGATAAAGGCTTCTCGTCGGACTCCAACTATTTGATCGGGGTTGGAAGCTACGACATCACTGGGCCGGCGGCGGATGTCAACATGATGGGATATGCCAACATGGAGCAGATTGCATCAGGAGTTCACTTCAGATTGCGAGCTCGTACATTCATTGTCGCGGAGCCACAAGGAGAAAGGGTTGTATTCGTTAATCTCGATGCTTGCATGGCCTCGCAGCTGGTGACGATCAAGGTGCTCCAGAGATTGAAGGCAAGGTATGGAAACCTCTACACTGAAAAAAATGTCGCCATTAGTGGAATCCATACTCATGCTGGACCAGGTGGTTATCTCCAGTACATTGTATACATAGTGACATCTCTTGGATTTGTGCGACAGTCATTTGATGCCCTTGTAAATGGTATTGAGAACAGCATTATAGAGGCTCATGAGAATCTGCGCCCAGGATCAATTTTCATAAACAAGGGAAAGCTGTTAGATGCAGGTGTAAACCGCAGCCCTAGTGCATATCTCAATAACCCTGGAGAAGAGCGTCGTAAATACAAATACAATGTTGACAAGCAGATGACACTTCTAAAATTTGTGGATCAGGAGTGGGGCCCTATTGGTAGCTTTAATTGGTTTTCGACTCATGGAACTTCTATGAGTCGTACAAACTCGTTAATTAGTGGGGACAACAAAGGTGCTGCTGCACGATTCATGGAGGACTGGTTTGAGAAAAAAGGAGGTATAAACTTGGATTTGGATATATCTGTAGATGACAGAATCCCACGAAGAGTCTCAAACATAATACCAGACCTGTCCATTAATTATCATGAGTTACTTGAGCTTGCTGTATCATTCCAATCTCCTTCCGGTAGATCAGCAACCAGAATTCTGAGTTCTTCAAAGCGTGTCAGGGGTGCCCTCAGGAATGCCGACAAACCCCGATTTGTGTCTGCATTTTGTCAATCAAACTGCGGTGATGTTAGCCCAAATGTTCTTGGAACATTCTGTATAGATACTGGACTTCCATGTGATTTCAATCACAGTACCTGTGATGGGAAGAATGAGCTATGCTACGGCAGAGGACCAGGTTATCCAGATGAATTCGAAAGCACGCGGATTATAGGAAAGAGACAATTCAGAAAAGCAGTTGATCTATTTAGTCATGCATCAGAGCAGTTAACAGGAAAGGTTGATTACCGTCACACATATGTAGACTTCTCTCAGCTTGAGGTAACTCTTGAACGTGGAGGAACTACTGAAGTGGTACAAACATGTCCTGCTGCAATGGGTTTCGCATTTGCCGCTGGAACCACTGATGGACCAGGTGCTTTTGATTTTGAGCAAGGAGATGACAAGGGAAATCCTTTTTGGAGGTTCATACGCAATCTTTTGAAGAAACCAGGGAAGGAACAAGTTGATTGTCAACAGCCAAAGCCCATCTTGCTGGATACTGGTGAAATGAGAAAACCATATGAGTGGGCTCCTTCAATACTTCCCATCCAAATCCTCAGAGTAGGGCAGCTAATCATTCTTGGAGTACCTGGAGAATTCACAACAATGGCTGGAAGGCGCCTTCGTGATGCTGTGAAGACCGTGCTTACCGATGGAGATAAAAATGGATTAAGTGGCGACATTCATATTGTTATTGCAGGACTAACAAATTCTTATTCACAGTATGTCACCACTTTCGAAGAGTACCAGGTGCAGAGATATGAGGGCGCTTCTACGTTATACGGTCCTCACACTCTCAGTGCCTACATTCAGGAGTTTAAGAAGCTTGCCTGTGCACTCATAGATGGCCGAGAAGTTGGCGCAGGGCCACAACCTCCAGATTTACTAGACAAGCAGATAGGCTTACTACCACCTGTTGTGATGGATACGACTCCTACTTCAATAAAATTTGGGGATATAAGATCTGATGTTCCCCAGAATTCCACCTTCAACAGAGGCGATATGGTGACGGTTACCTTCTGGTCAGCCTGTCCTCGGAACGATCTTATGACTGAAGGTACGTTTGCCCTGGTGGAGATTCTCCACAGCAAAAAGCTGTGGGTTCCAGCCTACGATGATGATGATTTCTGCCTGCGTTTCAAGTGGTCGAGACCGTTCAAACTTAGCAGTCGAAGTCATGCAACCATAGAATGGAGAATCCCTAAAACTGCAATTCCAGGAATGTACAGAATAACACACTTTGGGGCAGCAAAAAGTCTTTTCGGATCAATCCACCATTTCACAGGCTCGTCTAGTCCTTTTGGTGTAGCG

Coding sequence (CDS)

ATGGAAGTTCCTTCTCTGTTTAATTTCATCATTCGGAGACCGTGTGAAGCTCTGTTTTGGACTTGTGCATTACTACTGCTGCAGAATGATAAAGGCTTCTCGTCGGACTCCAACTATTTGATCGGGGTTGGAAGCTACGACATCACTGGGCCGGCGGCGGATGTCAACATGATGGGATATGCCAACATGGAGCAGATTGCATCAGGAGTTCACTTCAGATTGCGAGCTCGTACATTCATTGTCGCGGAGCCACAAGGAGAAAGGGTTGTATTCGTTAATCTCGATGCTTGCATGGCCTCGCAGCTGGTGACGATCAAGGTGCTCCAGAGATTGAAGGCAAGGTATGGAAACCTCTACACTGAAAAAAATGTCGCCATTAGTGGAATCCATACTCATGCTGGACCAGGTGGTTATCTCCAGTACATTGTATACATAGTGACATCTCTTGGATTTGTGCGACAGTCATTTGATGCCCTTGTAAATGGTATTGAGAACAGCATTATAGAGGCTCATGAGAATCTGCGCCCAGGATCAATTTTCATAAACAAGGGAAAGCTGTTAGATGCAGGTGTAAACCGCAGCCCTAGTGCATATCTCAATAACCCTGGAGAAGAGCGTCGTAAATACAAATACAATGTTGACAAGCAGATGACACTTCTAAAATTTGTGGATCAGGAGTGGGGCCCTATTGGTAGCTTTAATTGGTTTTCGACTCATGGAACTTCTATGAGTCGTACAAACTCGTTAATTAGTGGGGACAACAAAGGTGCTGCTGCACGATTCATGGAGGACTGGTTTGAGAAAAAAGGAGGTATAAACTTGGATTTGGATATATCTGTAGATGACAGAATCCCACGAAGAGTCTCAAACATAATACCAGACCTGTCCATTAATTATCATGAGTTACTTGAGCTTGCTGTATCATTCCAATCTCCTTCCGGTAGATCAGCAACCAGAATTCTGAGTTCTTCAAAGCGTGTCAGGGGTGCCCTCAGGAATGCCGACAAACCCCGATTTGTGTCTGCATTTTGTCAATCAAACTGCGGTGATGTTAGCCCAAATGTTCTTGGAACATTCTGTATAGATACTGGACTTCCATGTGATTTCAATCACAGTACCTGTGATGGGAAGAATGAGCTATGCTACGGCAGAGGACCAGGTTATCCAGATGAATTCGAAAGCACGCGGATTATAGGAAAGAGACAATTCAGAAAAGCAGTTGATCTATTTAGTCATGCATCAGAGCAGTTAACAGGAAAGGTTGATTACCGTCACACATATGTAGACTTCTCTCAGCTTGAGGTAACTCTTGAACGTGGAGGAACTACTGAAGTGGTACAAACATGTCCTGCTGCAATGGGTTTCGCATTTGCCGCTGGAACCACTGATGGACCAGGTGCTTTTGATTTTGAGCAAGGAGATGACAAGGGAAATCCTTTTTGGAGGTTCATACGCAATCTTTTGAAGAAACCAGGGAAGGAACAAGTTGATTGTCAACAGCCAAAGCCCATCTTGCTGGATACTGGTGAAATGAGAAAACCATATGAGTGGGCTCCTTCAATACTTCCCATCCAAATCCTCAGAGTAGGGCAGCTAATCATTCTTGGAGTACCTGGAGAATTCACAACAATGGCTGGAAGGCGCCTTCGTGATGCTGTGAAGACCGTGCTTACCGATGGAGATAAAAATGGATTAAGTGGCGACATTCATATTGTTATTGCAGGACTAACAAATTCTTATTCACAGTATGTCACCACTTTCGAAGAGTACCAGGTGCAGAGATATGAGGGCGCTTCTACGTTATACGGTCCTCACACTCTCAGTGCCTACATTCAGGAGTTTAAGAAGCTTGCCTGTGCACTCATAGATGGCCGAGAAGTTGGCGCAGGGCCACAACCTCCAGATTTACTAGACAAGCAGATAGGCTTACTACCACCTGTTGTGATGGATACGACTCCTACTTCAATAAAATTTGGGGATATAAGATCTGATGTTCCCCAGAATTCCACCTTCAACAGAGGCGATATGGTGACGGTTACCTTCTGGTCAGCCTGTCCTCGGAACGATCTTATGACTGAAGGTACGTTTGCCCTGGTGGAGATTCTCCACAGCAAAAAGCTGTGGGTTCCAGCCTACGATGATGATGATTTCTGCCTGCGTTTCAAGTGGTCGAGACCGTTCAAACTTAGCAGTCGAAGTCATGCAACCATAGAATGGAGAATCCCTAAAACTGCAATTCCAGGAATGTACAGAATAACACACTTTGGGGCAGCAAAAAGTCTTTTCGGATCAATCCACCATTTCACAGGCTCGTCTAGTCCTTTTGGTGTAGCG

Protein sequence

MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA

Homology

[Close]

BLAST of MS014232 vs. NCBI nr
Match: XP_022148376.1 (neutral ceramidase 1-like [Momordica charantia])

HSP 1 Score: 1586.6 bits (4107), Expect = 0.0e+00
Identity = 775/778 (99.61%), Postives = 776/778 (99.74%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY
Sbjct: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT
Sbjct: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKGKLLDAGVNRSPSAYLNNPGEE RKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPGEELRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC
Sbjct: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK
Sbjct: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTLSAYIQEFKKLACALIDGREV AGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD
Sbjct: 601 LYGPHTLSAYIQEFKKLACALIDGREVDAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           IRSDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR
Sbjct: 661 IRSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA 779
           FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA
Sbjct: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA 778

[Close]

BLAST of MS014232 vs. NCBI nr
Match: KAG6605432.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1441.0 bits (3729), Expect = 0.0e+00
Identity = 697/777 (89.70%), Postives = 736/777 (94.72%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF IR+P EALFW CA+LL+QN KG SSDSNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSIRKPYEALFWICAVLLVQNSKGSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIA GVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLK RYG+LYT
Sbjct: 61  ANMEQIAGGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKG+LLDAGVNRSPSAYLNNP EER+KY YNVDK+MTLLKFVD EWGPIGSFNWF+THG
Sbjct: 181 INKGELLDAGVNRSPSAYLNNPAEERQKYDYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GI  DLDISV+D IPRRVSNIIPDL IN+H
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKCGIASDLDISVEDTIPRRVSNIIPDLFINHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQSPSGR ATR LSSS+RVRGALRNADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQSPSGRPATRTLSSSRRVRGALRNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTC GKNELCYGRGPGYPDEFESTRIIG+RQFRKAVDLFS+A EQL GK
Sbjct: 361 IDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSNAPEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VD+RHTYVDFS+LEVTLERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDFRHTYVDFSKLEVTLERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LK P KEQVDCQ+PKPILLDTGEM KPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRIIRNFLKTPKKEQVDCQRPKPILLDTGEMTKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDG K+ LSGD+H+VIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGSKSELSGDVHVVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTL+AYIQEFKKLA ALIDG+ VGAGPQPPDLL+KQI LLPPVVMD TP S+KFGD
Sbjct: 601 LYGPHTLNAYIQEFKKLAHALIDGQAVGAGPQPPDLLNKQISLLPPVVMDATPRSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGT+ALVE+LH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPENSTFNRGDMVTVTFWSACPRNDLMTEGTYALVEVLHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSS+S+ATIEWRIPKTA PG+YRITHFGAAK LFGSI+HFTGSS+ FGV
Sbjct: 721 FKWSRPFKLSSKSYATIEWRIPKTATPGVYRITHFGAAKVLFGSINHFTGSSTAFGV 777

[Close]

BLAST of MS014232 vs. NCBI nr
Match: KAG7029416.1 (Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 697/777 (89.70%), Postives = 736/777 (94.72%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF IR+P EALFW CA+LL+QN KG SSDSNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSIRKPYEALFWICAVLLVQNSKGSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIA GVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLK RYG+LYT
Sbjct: 61  ANMEQIAGGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKG+LLDAGVNRSPSAYLNNP EER+KY YNVDK+MTLLKFVD EWGPIGSFNWF+THG
Sbjct: 181 INKGELLDAGVNRSPSAYLNNPAEERQKYDYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GI  DLDISV+D IPRRVSNIIPDL IN+H
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKCGIASDLDISVEDTIPRRVSNIIPDLFINHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQSPSGR ATR LSSS+RVRGALRNADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQSPSGRPATRTLSSSRRVRGALRNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTC GKNELCYGRGPGYPDEFESTRIIG+RQFRKAVDLFS+A EQL GK
Sbjct: 361 IDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSNAPEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VD+RHTYVDFS+LEVTLERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDFRHTYVDFSKLEVTLERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LK P KEQVDCQ+PKPILLDTGEM KPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRIIRNFLKTPKKEQVDCQRPKPILLDTGEMTKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDG K+ LSGD+H+VIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGSKSELSGDVHVVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTL+AYIQEFKKLA ALIDG+ VGAGPQPPDLL+KQI LLPPVVMD TP S+KFGD
Sbjct: 601 LYGPHTLNAYIQEFKKLAHALIDGQAVGAGPQPPDLLNKQISLLPPVVMDATPRSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGT+ALVE+LH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTYALVEVLHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSS+S+ATIEWRIPKTA PG+YRITHFGAAK LFGSI+HFTGSS+ FGV
Sbjct: 721 FKWSRPFKLSSKSYATIEWRIPKTATPGVYRITHFGAAKVLFGSINHFTGSSTAFGV 777

[Close]

BLAST of MS014232 vs. NCBI nr
Match: XP_023532163.1 (neutral ceramidase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 699/777 (89.96%), Postives = 737/777 (94.85%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF IR+P EALFW CA+LL++N KG SS SNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSIRKPYEALFWICAVLLVRNSKGSSSYSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIA GVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLK RYG+LYT
Sbjct: 61  ANMEQIAGGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKG+LLDAGVNRSPSAYLNNP EER+KY YNVDK+MTLLKFVD EWGPIGSFNWF+THG
Sbjct: 181 INKGELLDAGVNRSPSAYLNNPAEERQKYDYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GI  DLDISV+D IPRRVSNIIPDL IN+H
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKCGIASDLDISVEDTIPRRVSNIIPDLFINHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQSPSGR ATRILSSS+RVRGALRNADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQSPSGRPATRILSSSRRVRGALRNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIG+RQFRKAVDLFS+A EQL GK
Sbjct: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSNALEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VD+RHTYVDFS+LEVTLERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDFRHTYVDFSKLEVTLERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LK P KEQVDCQ+PKPILLDTGEM KPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRIIRNFLKTPKKEQVDCQRPKPILLDTGEMTKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDG K+ LSGD+HIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGSKSELSGDVHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTLSAYIQEFKKLA ALIDG+ VGAGPQPPDLL+KQI LLPPVVMD TP S+KFGD
Sbjct: 601 LYGPHTLSAYIQEFKKLAHALIDGQAVGAGPQPPDLLNKQISLLPPVVMDATPRSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGT+ALVE+LH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTYALVEVLHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSS+S+ATIEWRIPKTA PG+YRITHFGAAK LFGSI+HFTGSS+ FGV
Sbjct: 721 FKWSRPFKLSSQSYATIEWRIPKTATPGVYRITHFGAAKVLFGSINHFTGSSTVFGV 777

[Close]

BLAST of MS014232 vs. NCBI nr
Match: XP_038901468.1 (neutral ceramidase 1 [Benincasa hispida])

HSP 1 Score: 1439.5 bits (3725), Expect = 0.0e+00
Identity = 695/777 (89.45%), Postives = 733/777 (94.34%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF I+RPCEALFW CA LL+QN +G SSDSNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSIQRPCEALFWICAFLLVQNSEGSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQ ASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYG+LYT
Sbjct: 61  ANMEQTASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYI+YIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKGKLLDAGVNRSPSAYLNNP  ERRKYKYNVDK+MTLLKFVD EWGPIGSF+WF+THG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPAAERRKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKKGGI    DISVDDRIPRRVSNIIP+L  N+H
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGIGSGFDISVDDRIPRRVSNIIPNLFTNHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQ PSG SATRILSSS+RVRGAL NADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQRPSGTSATRILSSSRRVRGALTNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           +DTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLF++ASEQL GK
Sbjct: 361 VDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFNNASEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VDYRH+YVDFS+LEVTLERGGTT+VVQTCPAAMG++FAAGTTDGPGAFDF+QGDDKGNPF
Sbjct: 421 VDYRHSYVDFSKLEVTLERGGTTQVVQTCPAAMGYSFAAGTTDGPGAFDFKQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LKKP KEQ+DCQ PKPILLDTGEM KPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRLIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           F+TMAGR+LRDAVKTVLTDG K+ LSGDIH+VIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FSTMAGRQLRDAVKTVLTDGGKSELSGDIHVVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTLSAYIQEFKKLA AL+DG+ V AGPQPPDLLDKQ+ LLP VVMDTTP S+KFGD
Sbjct: 601 LYGPHTLSAYIQEFKKLARALVDGQAVAAGPQPPDLLDKQLSLLPSVVMDTTPHSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTF RGDMVTVTFWSACPRNDL+TEGTFALVEILH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFKRGDMVTVTFWSACPRNDLLTEGTFALVEILHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSSRSHATIEWRIPKTA  G+YRITHFGAAK LFGSIHHFTGSSS F V
Sbjct: 721 FKWSRPFKLSSRSHATIEWRIPKTASAGVYRITHFGAAKRLFGSIHHFTGSSSAFCV 777

[Close]

BLAST of MS014232 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 573/764 (75.00%), Postives = 651/764 (85.21%), Query Frame = 0


Query: 17  ALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRA 76
           + F+   +L L    G  S S YLIG+GSYDITGPAADVNMMGYANMEQ+ASG+HFRLRA
Sbjct: 15  SFFYISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRA 74

Query: 77  RTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPG 136
           RTFIV+EPQG+RVVFVNLDACMASQ+V +KV++RLKARYG+LYTE+NV ISGIHTHAGPG
Sbjct: 75  RTFIVSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPG 134

Query: 137 GYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPS 196
           GYLQY+VYIVTSLGFVRQSFDALV+GIENSII+AHENLRPGSIF+N G+LLDAGVNRSPS
Sbjct: 135 GYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPS 194

Query: 197 AYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKG 256
           AYLNNP +ER K+KYNVDK+MTLLKFVD +WGP+GSFNWF+THGTSMSRTNSLISGDNKG
Sbjct: 195 AYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKG 254

Query: 257 AAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRS 316
           AA+RFMEDW+E+        +  + D IPRRVS++I +   ++HELLELA  F+S  G+ 
Sbjct: 255 AASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKP 314

Query: 317 ATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDG 376
            TRI SS++RVR ALR ADKP FVSAFCQ+NCGDVSPNVLG FC+DTGLPCDFNHSTC G
Sbjct: 315 VTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGG 374

Query: 377 KNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVT 436
           KNE+CYGRGPGYPDEFESTRIIG+RQF+ A++LF+ ASEQL GKVDYRH YVDFSQL VT
Sbjct: 375 KNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVT 434

Query: 437 L-ERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQ 496
           L ++ G +EVV+TCPAAMGFAFAAGTTDGPGAFDF QGDDKGNPFWR +RN+LK P K+Q
Sbjct: 435 LPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQ 494

Query: 497 VDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKT 556
           +DC  PKPILLDTGEM KPY+WAPSIL +Q+LR+GQL IL VPGEFTTMAGRRLR AVKT
Sbjct: 495 IDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKT 554

Query: 557 VLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFK 616
            L +     LSG+IH+VIAGL N YSQYVTTFEEYQVQRYEGASTLYGPHTLS YIQEFK
Sbjct: 555 QLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFK 614

Query: 617 KLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGD 676
           KL+ +L+    V  GPQPPDLLDKQ+  L PV+MDTTP+   FGD+ SDVP+N +  RG+
Sbjct: 615 KLSKSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGN 674

Query: 677 -MVTVTFWSACPRNDLMTEGTFALVEILHSK-KLWVPAYDDDDFCLRFKWSRPFKLSSRS 736
             VTV F SACPRNDL+TEGTF LVE L  K K W P YDDDD CLRFKWSR  KLSSRS
Sbjct: 675 GQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 734

Query: 737 HATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
            AT+EWRIP++A PG+YRITHFGAAK LFGS+HHFTGSSS F V
Sbjct: 735 QATVEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778

[Close]

BLAST of MS014232 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 547/769 (71.13%), Postives = 636/769 (82.70%), Query Frame = 0


Query: 20  WTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTF 79
           W    L+L N     S S YL+G+GS+DITGPAADVNMMGYAN EQIASG+HFRL++R F
Sbjct: 22  WLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAF 81

Query: 80  IVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYL 139
           IVAEP G+RVVFVN+DACMASQ+VTIKVL+RLKARYG+LY E NVAISGIHTHAGPGGYL
Sbjct: 82  IVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGGYL 141

Query: 140 QYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYL 199
           QY+VYIVTSLGFVRQSFD +V+GIE SI+EAH NLRPG IF+NKG LLDAGVNRSPSAYL
Sbjct: 142 QYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSAYL 201

Query: 200 NNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAA 259
           NNP EER KY+YNVDK+MTL+KFVD E GP+GSFNWF+THGTSMSRTNSLISGDNKGAAA
Sbjct: 202 NNPAEERSKYEYNVDKEMTLIKFVDDELGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 261

Query: 260 RFMEDWFEKKGGINLDLDISVDDR--------IPRRVSNIIPDLSINYHELLELAVSFQS 319
           RFMEDW E+ G        + DD         +PRRVS IIP+ +    +L++LA S+++
Sbjct: 262 RFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEA 321

Query: 320 PSGRSATRILSSS--KRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDF 379
             GR   R+  SS  +R+R   +N  KP+FVSAFCQSNCGDVSPNVLGTFCIDT LPCDF
Sbjct: 322 SGGR---RLAGSSITRRIRSTQQN--KPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDF 381

Query: 380 NHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVD 439
           NHSTC+GKNELCYGRGPGYPDEFESTR+IG RQF KA DLF  ASE++ GK+DYRHTY+D
Sbjct: 382 NHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLD 441

Query: 440 FSQLEVTLE-RGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLL 499
           FS+LEV +    G  + V+TCPAAMGFAFAAGTTDGPGAFDF QGD KGNPFW+ +RNLL
Sbjct: 442 FSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLL 501

Query: 500 KKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRR 559
           K PGK+QV+C  PKPILLDTGEM++PY+WAP+ILP+Q++R+GQL+IL VPGEFTTMAGRR
Sbjct: 502 KTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRR 561

Query: 560 LRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLS 619
           LRDAVKTVLT G+      +IH+V+AGLTNSYSQY+TTFEEYQ+QRYEGASTLYGPHTLS
Sbjct: 562 LRDAVKTVLTSGNSE-FDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLS 621

Query: 620 AYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQN 679
           AYIQEF+KLA A+I  +EV    QPPD+LDKQIGLLP VV D+TP  +KFGD+ SDVP N
Sbjct: 622 AYIQEFQKLAMAMIANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDVPGN 681

Query: 680 STFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLRFKWSRPFK 739
           STFN+G  V  TF+SACPRNDL+T+GTFALVE L     WVP YDDDD+ LRFKWSRP +
Sbjct: 682 STFNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPAR 741

Query: 740 LSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           LSSRS AT+EW +P+ A  G+YR+ HFGA+K +FGS+ HFTG+S  F V
Sbjct: 742 LSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784

[Close]

BLAST of MS014232 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 544/741 (73.41%), Postives = 625/741 (84.35%), Query Frame = 0


Query: 39  YLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACM 98
           YLIGVGSYDITGPAADVNMMGYAN +QIASG+HFRLRAR FIVAEPQG RVVFVNLDACM
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGNRVVFVNLDACM 85

Query: 99  ASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDA 158
           ASQ+VTIKVL+RLKARYG LYTEKNVAISGIHTHAGPGGYLQY+ YIVTSLGFVRQSFD 
Sbjct: 86  ASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDV 145

Query: 159 LVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMT 218
           +VNGIE SI++AHE+LRPGS F+NKG LLDAGVNRSPS+YLNNP  ER KYKY+VDK+MT
Sbjct: 146 VVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMT 205

Query: 219 LLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDI 278
           L+KFVD + GP GSFNWF+THGTSMSRTNSLISGDNKGAAARFMEDWFE  G  N     
Sbjct: 206 LVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFE-NGQKN----- 265

Query: 279 SVDDR-IPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKP 338
           SV  R IPRRVS I+ D S N   LL++A +++S  G S  + L    RV    RN  K 
Sbjct: 266 SVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRV----RNGSKR 325

Query: 339 RFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRI 398
           +FVSAFCQSNCGDVSPN LGTFCIDTGLPCDFNHSTC+G+NELCYGRGPGYPDEFESTRI
Sbjct: 326 KFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRI 385

Query: 399 IGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLER-GGTTEVVQTCPAAMGFA 458
           IG++QF+ AV+LF+ A+E+L GK+ Y+H Y+DFS L+VT+ + GG +E V+TCPAAMGF 
Sbjct: 386 IGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFG 445

Query: 459 FAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYE 518
           FAAGTTDGPGAFDF+QGDD+GN FWR +RN+L+ PG EQV CQ+PKPILLDTGEM++PY+
Sbjct: 446 FAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYD 505

Query: 519 WAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGL 578
           WAPSILPIQILR+GQL+IL VPGEFTTMAGRRLRDA+K+ L   D    S ++H+VIAGL
Sbjct: 506 WAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGL 565

Query: 579 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDL 638
           TN+YSQY+ TFEEY+VQRYEGASTLYG HTL+AYIQEFKKLA AL++G  +  GPQPPDL
Sbjct: 566 TNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDL 625

Query: 639 LDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTF 698
           LDKQI LL PVV+D+TP  +KFGD+++DVP  STF RG  V  TFWS CPRNDLMTEG+F
Sbjct: 626 LDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSF 685

Query: 699 ALVEILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFG 758
           A+VE L     W P YDDDDF L+FKWSRP KLSS S ATIEWR+P++A+ G+YRI H+G
Sbjct: 686 AVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYG 745

Query: 759 AAKSLFGSIHHFTGSSSPFGV 778
           A+KSLFGSI  F+GSSS F V
Sbjct: 746 ASKSLFGSISSFSGSSSAFVV 756

[Close]

BLAST of MS014232 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 1.1e-309
Identity = 536/760 (70.53%), Postives = 610/760 (80.26%), Query Frame = 0


Query: 21  TCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFI 80
           T  LL L       SDS+YL+G+GSYDITGPAADVNMMGYANMEQ+ASGVHFRLRAR FI
Sbjct: 11  TLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFI 70

Query: 81  VAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQ 140
           VAEP  +R+ FVNLDA MASQLVTIKV++RLK RYG LYTE+NVAISG HTHAGPGGYLQ
Sbjct: 71  VAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQ 130

Query: 141 YIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLN 200
           YI+Y+VTSLGFV QSF+ALV+GIE SII+AHENLRPGSI INKG+LLDAGVNRSPSAYLN
Sbjct: 131 YILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLN 190

Query: 201 NPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAAR 260
           NP  ER KY+Y+VDK+MTL+KFVD +WGP+                            AR
Sbjct: 191 NPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------AR 250

Query: 261 FMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRI 320
            MEDWFE++ G       SVD   PRRVS+II D      +L+E+A S  S  G++ TR+
Sbjct: 251 IMEDWFERENGCR-----SVDVESPRRVSSIISD--PYDQDLMEMASSLLSTGGKTVTRM 310

Query: 321 LSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNEL 380
            S ++RVR   R+ADKPRFVSAFCQ+NCGDVSPNVLG FCIDTGLPC+FN STC GKNE 
Sbjct: 311 SSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQ 370

Query: 381 CYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLE-R 440
           CYGRGPGYPDEFESTRIIG+RQF+KA DLF+ ASE++ GKVDYRH YVDFSQLEVT+  +
Sbjct: 371 CYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQ 430

Query: 441 GGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQVDCQ 500
            G +EVV+TCPAAMGF FAAGTTDGPGAFDF+QGDD+GNPFWR +RNLLK P +EQV CQ
Sbjct: 431 NGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQ 490

Query: 501 QPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTD 560
           +PKPILLDTGEM++PY+WAPSILP+QILR+GQL+IL VPGEFTTMAGRRLRDAVKTVL +
Sbjct: 491 RPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKE 550

Query: 561 GDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLAC 620
           G  NG   +  +VIAGLTNSYSQY+ TFEEYQVQRYEGASTLYGPHTLS YIQEFKKLA 
Sbjct: 551 G-SNG--REFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLAN 610

Query: 621 ALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRG-DMVT 680
            L+  +    GPQPPDLL KQI LL PVV D TP    FGD+ SDVP+ S F +G D+V 
Sbjct: 611 DLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADIVR 670

Query: 681 VTFWSACPRNDLMTEGTFALVE-ILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATI 740
           V F SA PRNDLMTEGTFALVE  L  ++ WVP YDDDDFCLRFKWSRPFKLS++S ATI
Sbjct: 671 VQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATI 730

Query: 741 EWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           EWRIP+TA PG+YRITHFG+AK+   SIHHF+GSSS F V
Sbjct: 731 EWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732

[Close]

BLAST of MS014232 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 8.6e-177
Identity = 328/730 (44.93%), Postives = 446/730 (61.10%), Query Frame = 0


Query: 34  SSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPQGERVVFVN 93
           S DS+Y IG G YDITG +A+VN+MGYAN  Q+ +G+HFR RAR F+  +  G R V+V+
Sbjct: 41  SGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDSNGNRAVYVS 100

Query: 94  LDACMASQLVTIKVLQRLKARYG-NLYTEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFV 153
            D+CM  Q V I V++ L+  +G N+YTE NV +SG HTH+GP G+ QY +Y +TSLGF 
Sbjct: 101 TDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFY 160

Query: 154 RQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPGEERRKYKYN 213
           +++FD + NGI  +I++AH++++P ++F   G+L +  +NRSP AY NNP EE+  Y  N
Sbjct: 161 KKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSN 220

Query: 214 VDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGGI 273
           VDK MT+L+  D    P  + ++F+ H TSM+ TN LISGDNKG A+   E         
Sbjct: 221 VDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWE--------- 280

Query: 274 NLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRILSSSKRVRGALR 333
                                                               K+V G   
Sbjct: 281 ----------------------------------------------------KQVNGP-G 340

Query: 334 NADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNELCYGRGPGYP-DE 393
            A K  FV+AF QSN GDVSPN  G  C D G PCD+  STC+G+NE C+  GPG   D 
Sbjct: 341 TAGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDM 400

Query: 394 FESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLERGGTTEVVQTCPA 453
           FEST+IIG  QF KA++LF++AS Q++G V YRH++V F+ + V        +   TC  
Sbjct: 401 FESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRG 460

Query: 454 AMGFAFAAGTTDGPGAFDFEQGDD--KGNPFWRFIRNLLKKPGKEQVDCQQPKPILLDTG 513
           AMG++FAAGTTDGPGAF+F Q D+   GNPFW FI + + KP  +Q+ CQ PKPILLD G
Sbjct: 461 AMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG 520

Query: 514 EMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTDGDKNGLSGDI 573
            M +P  W P ++PIQI+ +GQ++++ VPGEFTTM+GRRLR++V+ ++ +  +N +    
Sbjct: 521 -MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREIIGESIENPI---- 580

Query: 574 HIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLACALIDGREVGA 633
            ++IAGL+N+YS Y+ TFEEYQVQRYEGAST++GPHTL +Y+QEF KLA +++DG  V A
Sbjct: 581 -VLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPA 640

Query: 634 GPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGDMVTVTFWSACPRND 693
           GP P +L    +  LPPV++D  P    FG++  DV  N + N  + V+  F+   PRND
Sbjct: 641 GPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNLNYSVN--ETVSCVFYGGNPRND 697

Query: 694 LMTEGTFALVEILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATIEWRIPKTAIPGM 753
            M E +F  V++L     W    DD D+  +FKW         S  TIEW I     PG 
Sbjct: 701 FMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWK--MHDLGFSLITIEWVIAPDTTPGT 697

Query: 754 YRITHFGAAK 760
           YRITH G AK
Sbjct: 761 YRITHSGFAK 697

[Close]

BLAST of MS014232 vs. ExPASy TrEMBL
Match: A0A6J1D565 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111017041 PE=3 SV=1)

HSP 1 Score: 1586.6 bits (4107), Expect = 0.0e+00
Identity = 775/778 (99.61%), Postives = 776/778 (99.74%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY
Sbjct: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT
Sbjct: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKGKLLDAGVNRSPSAYLNNPGEE RKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPGEELRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC
Sbjct: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK
Sbjct: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTLSAYIQEFKKLACALIDGREV AGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD
Sbjct: 601 LYGPHTLSAYIQEFKKLACALIDGREVDAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           IRSDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR
Sbjct: 661 IRSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA 779
           FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA
Sbjct: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGVA 778

[Close]

BLAST of MS014232 vs. ExPASy TrEMBL
Match: A0A6J1G7Q7 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111451504 PE=3 SV=1)

HSP 1 Score: 1438.3 bits (3722), Expect = 0.0e+00
Identity = 695/777 (89.45%), Postives = 737/777 (94.85%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF IR+P EALFW CA+LL+QN KG SSDSNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSIRKPYEALFWICAVLLVQNSKGSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQIA GVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLK RYG+LYT
Sbjct: 61  ANMEQIAGGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKG+LLDAGVNRSPSAYLNNP EER+KY YNVDK+MTLLKFVD EWGPIGSFNWF+THG
Sbjct: 181 INKGELLDAGVNRSPSAYLNNPAEERQKYDYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TS+SRTNSLISGDNKGAAARFMEDWFEKK GI  DLDISV+D IPRRVSNIIPDL IN+H
Sbjct: 241 TSLSRTNSLISGDNKGAAARFMEDWFEKKCGIASDLDISVEDTIPRRVSNIIPDLFINHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQSPSGR ATRILSSS+RVRGALRNADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQSPSGRPATRILSSSRRVRGALRNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTC GKNELCYGRGPGYPDEFESTRIIG+RQFRKAV+LFS+A EQL GK
Sbjct: 361 IDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVELFSNAPEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VD+RHTYVDFS+LEVTLERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDFRHTYVDFSKLEVTLERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LK P KEQVDCQ+PKPILLDTGEM KPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRIIRNFLKTPKKEQVDCQRPKPILLDTGEMTKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDG K+ LSGD+H+VIAGLTNSYSQYVTTFEE+QVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGSKSELSGDVHVVIAGLTNSYSQYVTTFEEFQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTL+AYIQEFKKLA ALIDG+ VGAGPQPPDLL+KQI LLPPVVMD TP S+KFGD
Sbjct: 601 LYGPHTLNAYIQEFKKLAHALIDGQAVGAGPQPPDLLNKQISLLPPVVMDATPRSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGT+ALVE+LH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTYALVEVLHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSS+S+ATIEWRIPKTA PG+YRITHFGAAK LFGSI+HFTGSS+ FGV
Sbjct: 721 FKWSRPFKLSSKSYATIEWRIPKTATPGVYRITHFGAAKVLFGSINHFTGSSTAFGV 777

[Close]

BLAST of MS014232 vs. ExPASy TrEMBL
Match: A0A6J1L802 (Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111500018 PE=3 SV=1)

HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 694/777 (89.32%), Postives = 734/777 (94.47%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGY 60
           ME  SLFNF  R+P E LFW CA+LL+QN KG SSDSNYLIG+GSYDITGPAADVNMMGY
Sbjct: 1   MEFASLFNFSFRKPYEVLFWICAVLLVQNSKGSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYT 120
           ANMEQ A GVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLK RYG+LYT
Sbjct: 61  ANMEQTAGGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSF+ LVNGIENSII+AHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFNVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHG 240
           INKG+LLDAGVNRSPSAYLNNP EER+KY YNVDK+MTLLKFVD EWGPIGSFNWF+THG
Sbjct: 181 INKGELLDAGVNRSPSAYLNNPAEERQKYDYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GI  DLDISV+D IPRRVSNIIPDL IN+H
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKCGIASDLDISVEDTIPRRVSNIIPDLFINHH 300

Query: 301 ELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFC 360
           ELLELA SFQSPSGRSATRILSSS+RVRGALRNADKP+FVSAFCQSNCGDVSPNVLG FC
Sbjct: 301 ELLELAASFQSPSGRSATRILSSSRRVRGALRNADKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGK 420
           IDTGLPCDFNHSTC GKNELCYGRGPGYPDEFESTRIIG+RQF KAVDL S+A EQL GK
Sbjct: 361 IDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFIKAVDLLSNAPEQLKGK 420

Query: 421 VDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPF 480
           VD+RHTYVDFS+LEVTLERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDFRHTYVDFSKLEVTLERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGE 540
           WR IRN LK P KEQVDCQ+PKPILLDTGEMRKPY+WAPSILPIQILRVGQLIILGVPGE
Sbjct: 481 WRIIRNFLKTPKKEQVDCQRPKPILLDTGEMRKPYDWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FTTMAGRRLRDAVKTVLTDG K+ L+GD+HIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FTTMAGRRLRDAVKTVLTDGSKSELNGDVHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGD 660
           LYGPHTL+AYIQEFKKLA ALIDG+ VGAGPQPPDLL+KQI LLPPVVMD TP S+KFGD
Sbjct: 601 LYGPHTLNAYIQEFKKLAHALIDGQAVGAGPQPPDLLNKQISLLPPVVMDATPRSVKFGD 660

Query: 661 IRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLR 720
           ++SDVP+NSTFNRGDMVTVTFWSACPRNDLMTEGT+ALVE+LH KKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFNRGDMVTVTFWSACPRNDLMTEGTYALVEVLHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           FKWSRPFKLSS+S+ATIEWRIPKTA PG+YRITHFGAAK LFGSI+HFTGSS+ FGV
Sbjct: 721 FKWSRPFKLSSQSYATIEWRIPKTATPGVYRITHFGAAKVLFGSINHFTGSSTAFGV 777

[Close]

BLAST of MS014232 vs. ExPASy TrEMBL
Match: A0A5D3CV52 (Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00370 PE=3 SV=1)

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 693/778 (89.07%), Postives = 730/778 (93.83%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQN-DKGFSSDSNYLIGVGSYDITGPAADVNMMG 60
           ME  SLFNF IRRPCEALFW  A LLLQN +   SSDSNYLIG+GSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLY 120
           YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIK+LQRLK RYG+LY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYI+YIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTH 240
           FINKGKLLDAGVNRSPSAYLNNP EER KYKYNVDK+MTLLKFVD EWGPIGSF+WF+TH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINY 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKK GI+   +ISVDDRIPRRVS+IIPDL IN+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTF 360
           HELLELA SFQSPSGRSATRILSSS+RVRGALRNA+KP+FVSAFCQSNCGDVSPNVLG F
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTG 420
           CIDTGLPCDFNHSTC+GKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFS+ASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRH+YVDFS+LEVT+ERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPG 540
           FW+ IRN LKKP KEQ+DCQ PKPILLDTGEM KPY WAPSILPIQILRVGQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EF+TMAGRRLRDAVKTVLTDGDK+  S DIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600

Query: 601 TLYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFG 660
           TLYGPHTLSAYIQEFKKLA +L+DG+ V AGPQPPDLLDKQI  LP VVMDTTP S+KFG
Sbjct: 601 TLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DIRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCL 720
           D+ SDVP+NSTF RGD VTVTFWSACPRNDLMTEGT+ALVEILH KKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           RFKWSRPFKLSS+SHATIEWRIPK+A PG+YRITHFGAAK LFGSI HFTGSSS FGV
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGV 778

[Close]

BLAST of MS014232 vs. ExPASy TrEMBL
Match: A0A1S3CM50 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502048 PE=3 SV=1)

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 693/778 (89.07%), Postives = 730/778 (93.83%), Query Frame = 0


Query: 1   MEVPSLFNFIIRRPCEALFWTCALLLLQN-DKGFSSDSNYLIGVGSYDITGPAADVNMMG 60
           ME  SLFNF IRRPCEALFW  A LLLQN +   SSDSNYLIG+GSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLY 120
           YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIK+LQRLK RYG+LY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYI+YIVTSLGFVRQSFD LVNGIENSII+AHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTH 240
           FINKGKLLDAGVNRSPSAYLNNP EER KYKYNVDK+MTLLKFVD EWGPIGSF+WF+TH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINY 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKK GI+   +ISVDDRIPRRVS+IIPDL IN+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTF 360
           HELLELA SFQSPSGRSATRILSSS+RVRGALRNA+KP+FVSAFCQSNCGDVSPNVLG F
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTG 420
           CIDTGLPCDFNHSTC+GKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFS+ASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHTYVDFSQLEVTLERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRH+YVDFS+LEVT+ERGGTTEVVQTCPAAMG++FAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPG 540
           FW+ IRN LKKP KEQ+DCQ PKPILLDTGEM KPY WAPSILPIQILRVGQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EF+TMAGRRLRDAVKTVLTDGDK+  S DIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600

Query: 601 TLYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFG 660
           TLYGPHTLSAYIQEFKKLA +L+DG+ V AGPQPPDLLDKQI  LP VVMDTTP S+KFG
Sbjct: 601 TLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DIRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCL 720
           D+ SDVP+NSTF RGD VTVTFWSACPRNDLMTEGT+ALVEILH KKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           RFKWSRPFKLSS+SHATIEWRIPK+A PG+YRITHFGAAK LFGSI HFTGSSS FGV
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGV 778

[Close]

BLAST of MS014232 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 573/764 (75.00%), Postives = 651/764 (85.21%), Query Frame = 0


Query: 17  ALFWTCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRA 76
           + F+   +L L    G  S S YLIG+GSYDITGPAADVNMMGYANMEQ+ASG+HFRLRA
Sbjct: 15  SFFYISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRA 74

Query: 77  RTFIVAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPG 136
           RTFIV+EPQG+RVVFVNLDACMASQ+V +KV++RLKARYG+LYTE+NV ISGIHTHAGPG
Sbjct: 75  RTFIVSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPG 134

Query: 137 GYLQYIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPS 196
           GYLQY+VYIVTSLGFVRQSFDALV+GIENSII+AHENLRPGSIF+N G+LLDAGVNRSPS
Sbjct: 135 GYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPS 194

Query: 197 AYLNNPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKG 256
           AYLNNP +ER K+KYNVDK+MTLLKFVD +WGP+GSFNWF+THGTSMSRTNSLISGDNKG
Sbjct: 195 AYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKG 254

Query: 257 AAARFMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRS 316
           AA+RFMEDW+E+        +  + D IPRRVS++I +   ++HELLELA  F+S  G+ 
Sbjct: 255 AASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKP 314

Query: 317 ATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDG 376
            TRI SS++RVR ALR ADKP FVSAFCQ+NCGDVSPNVLG FC+DTGLPCDFNHSTC G
Sbjct: 315 VTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGG 374

Query: 377 KNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVT 436
           KNE+CYGRGPGYPDEFESTRIIG+RQF+ A++LF+ ASEQL GKVDYRH YVDFSQL VT
Sbjct: 375 KNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVT 434

Query: 437 L-ERGGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQ 496
           L ++ G +EVV+TCPAAMGFAFAAGTTDGPGAFDF QGDDKGNPFWR +RN+LK P K+Q
Sbjct: 435 LPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQ 494

Query: 497 VDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKT 556
           +DC  PKPILLDTGEM KPY+WAPSIL +Q+LR+GQL IL VPGEFTTMAGRRLR AVKT
Sbjct: 495 IDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKT 554

Query: 557 VLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFK 616
            L +     LSG+IH+VIAGL N YSQYVTTFEEYQVQRYEGASTLYGPHTLS YIQEFK
Sbjct: 555 QLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFK 614

Query: 617 KLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGD 676
           KL+ +L+    V  GPQPPDLLDKQ+  L PV+MDTTP+   FGD+ SDVP+N +  RG+
Sbjct: 615 KLSKSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGN 674

Query: 677 -MVTVTFWSACPRNDLMTEGTFALVEILHSK-KLWVPAYDDDDFCLRFKWSRPFKLSSRS 736
             VTV F SACPRNDL+TEGTF LVE L  K K W P YDDDD CLRFKWSR  KLSSRS
Sbjct: 675 GQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 734

Query: 737 HATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
            AT+EWRIP++A PG+YRITHFGAAK LFGS+HHFTGSSS F V
Sbjct: 735 QATVEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778

[Close]

BLAST of MS014232 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 544/741 (73.41%), Postives = 625/741 (84.35%), Query Frame = 0


Query: 39  YLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACM 98
           YLIGVGSYDITGPAADVNMMGYAN +QIASG+HFRLRAR FIVAEPQG RVVFVNLDACM
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGNRVVFVNLDACM 85

Query: 99  ASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDA 158
           ASQ+VTIKVL+RLKARYG LYTEKNVAISGIHTHAGPGGYLQY+ YIVTSLGFVRQSFD 
Sbjct: 86  ASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDV 145

Query: 159 LVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMT 218
           +VNGIE SI++AHE+LRPGS F+NKG LLDAGVNRSPS+YLNNP  ER KYKY+VDK+MT
Sbjct: 146 VVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMT 205

Query: 219 LLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDI 278
           L+KFVD + GP GSFNWF+THGTSMSRTNSLISGDNKGAAARFMEDWFE  G  N     
Sbjct: 206 LVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFE-NGQKN----- 265

Query: 279 SVDDR-IPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRILSSSKRVRGALRNADKP 338
           SV  R IPRRVS I+ D S N   LL++A +++S  G S  + L    RV    RN  K 
Sbjct: 266 SVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRV----RNGSKR 325

Query: 339 RFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRI 398
           +FVSAFCQSNCGDVSPN LGTFCIDTGLPCDFNHSTC+G+NELCYGRGPGYPDEFESTRI
Sbjct: 326 KFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRI 385

Query: 399 IGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLER-GGTTEVVQTCPAAMGFA 458
           IG++QF+ AV+LF+ A+E+L GK+ Y+H Y+DFS L+VT+ + GG +E V+TCPAAMGF 
Sbjct: 386 IGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFG 445

Query: 459 FAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYE 518
           FAAGTTDGPGAFDF+QGDD+GN FWR +RN+L+ PG EQV CQ+PKPILLDTGEM++PY+
Sbjct: 446 FAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYD 505

Query: 519 WAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGL 578
           WAPSILPIQILR+GQL+IL VPGEFTTMAGRRLRDA+K+ L   D    S ++H+VIAGL
Sbjct: 506 WAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGL 565

Query: 579 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDL 638
           TN+YSQY+ TFEEY+VQRYEGASTLYG HTL+AYIQEFKKLA AL++G  +  GPQPPDL
Sbjct: 566 TNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDL 625

Query: 639 LDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTF 698
           LDKQI LL PVV+D+TP  +KFGD+++DVP  STF RG  V  TFWS CPRNDLMTEG+F
Sbjct: 626 LDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSF 685

Query: 699 ALVEILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFG 758
           A+VE L     W P YDDDDF L+FKWSRP KLSS S ATIEWR+P++A+ G+YRI H+G
Sbjct: 686 AVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYG 745

Query: 759 AAKSLFGSIHHFTGSSSPFGV 778
           A+KSLFGSI  F+GSSS F V
Sbjct: 746 ASKSLFGSISSFSGSSSAFVV 756

[Close]

BLAST of MS014232 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 543/776 (69.97%), Postives = 626/776 (80.67%), Query Frame = 0


Query: 39  YLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACM 98
           YLIGVGSYDITGPAADVNMMGYAN +QIASG+HFRLRAR FIVAEPQG RVVFVNLDACM
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGNRVVFVNLDACM 85

Query: 99  ASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQYIVYIVTSLGFVRQSFDA 158
           ASQ+VTIKVL+RLKARYG LYTEKNVAISGIHTHAGPGGYLQY+ YIVTSLGFVRQSFD 
Sbjct: 86  ASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDV 145

Query: 159 LVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPGEERRKYKYNVDKQMT 218
           +VNGIE SI++AHE+LRPGS F+NKG LLDAGVNRSPS+YLNNP  ER KYKY+VDK+MT
Sbjct: 146 VVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMT 205

Query: 219 LLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGGINLDLDI 278
           L+KFVD + GP GSFNWF+THGTSMSRTNSLISGDNKGAAARFMEDWFE  G  N     
Sbjct: 206 LVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFE-NGQKN----- 265

Query: 279 SVDDR-IPRRVSNIIPDLS-----------------------------------INYHEL 338
           SV  R IPRRVS I+ D S                                   ++   L
Sbjct: 266 SVSSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRL 325

Query: 339 LELAVSFQSPSGRSATRILSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCID 398
           L++A +++S  G S  + L    RV    RN  K +FVSAFCQSNCGDVSPN LGTFCID
Sbjct: 326 LDIAATYKSSRGHSVDKSLDVKTRV----RNGSKRKFVSAFCQSNCGDVSPNTLGTFCID 385

Query: 399 TGLPCDFNHSTCDGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVD 458
           TGLPCDFNHSTC+G+NELCYGRGPGYPDEFESTRIIG++QF+ AV+LF+ A+E+L GK+ 
Sbjct: 386 TGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIG 445

Query: 459 YRHTYVDFSQLEVTLER-GGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFW 518
           Y+H Y+DFS L+VT+ + GG +E V+TCPAAMGF FAAGTTDGPGAFDF+QGDD+GN FW
Sbjct: 446 YQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFW 505

Query: 519 RFIRNLLKKPGKEQVDCQQPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEF 578
           R +RN+L+ PG EQV CQ+PKPILLDTGEM++PY+WAPSILPIQILR+GQL+IL VPGEF
Sbjct: 506 RLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEF 565

Query: 579 TTMAGRRLRDAVKTVLTDGDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTL 638
           TTMAGRRLRDA+K+ L   D    S ++H+VIAGLTN+YSQY+ TFEEY+VQRYEGASTL
Sbjct: 566 TTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTL 625

Query: 639 YGPHTLSAYIQEFKKLACALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDI 698
           YG HTL+AYIQEFKKLA AL++G  +  GPQPPDLLDKQI LL PVV+D+TP  +KFGD+
Sbjct: 626 YGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDV 685

Query: 699 RSDVPQNSTFNRGDMVTVTFWSACPRNDLMTEGTFALVEILHSKKLWVPAYDDDDFCLRF 758
           ++DVP  STF RG  V  TFWS CPRNDLMTEG+FA+VE L     W P YDDDDF L+F
Sbjct: 686 KADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKF 745

Query: 759 KWSRPFKLSSRSHATIEWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           KWSRP KLSS S ATIEWR+P++A+ G+YRI H+GA+KSLFGSI  F+GSSS F V
Sbjct: 746 KWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 791

[Close]

BLAST of MS014232 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1063.5 bits (2749), Expect = 7.9e-311
Identity = 536/760 (70.53%), Postives = 610/760 (80.26%), Query Frame = 0


Query: 21  TCALLLLQNDKGFSSDSNYLIGVGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFI 80
           T  LL L       SDS+YL+G+GSYDITGPAADVNMMGYANMEQ+ASGVHFRLRAR FI
Sbjct: 11  TLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFI 70

Query: 81  VAEPQGERVVFVNLDACMASQLVTIKVLQRLKARYGNLYTEKNVAISGIHTHAGPGGYLQ 140
           VAEP  +R+ FVNLDA MASQLVTIKV++RLK RYG LYTE+NVAISG HTHAGPGGYLQ
Sbjct: 71  VAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQ 130

Query: 141 YIVYIVTSLGFVRQSFDALVNGIENSIIEAHENLRPGSIFINKGKLLDAGVNRSPSAYLN 200
           YI+Y+VTSLGFV QSF+ALV+GIE SII+AHENLRPGSI INKG+LLDAGVNRSPSAYLN
Sbjct: 131 YILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLN 190

Query: 201 NPGEERRKYKYNVDKQMTLLKFVDQEWGPIGSFNWFSTHGTSMSRTNSLISGDNKGAAAR 260
           NP  ER KY+Y+VDK+MTL+KFVD +WGP+                            AR
Sbjct: 191 NPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------AR 250

Query: 261 FMEDWFEKKGGINLDLDISVDDRIPRRVSNIIPDLSINYHELLELAVSFQSPSGRSATRI 320
            MEDWFE++ G       SVD   PRRVS+II D      +L+E+A S  S  G++ TR+
Sbjct: 251 IMEDWFERENGCR-----SVDVESPRRVSSIISD--PYDQDLMEMASSLLSTGGKTVTRM 310

Query: 321 LSSSKRVRGALRNADKPRFVSAFCQSNCGDVSPNVLGTFCIDTGLPCDFNHSTCDGKNEL 380
            S ++RVR   R+ADKPRFVSAFCQ+NCGDVSPNVLG FCIDTGLPC+FN STC GKNE 
Sbjct: 311 SSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQ 370

Query: 381 CYGRGPGYPDEFESTRIIGKRQFRKAVDLFSHASEQLTGKVDYRHTYVDFSQLEVTLE-R 440
           CYGRGPGYPDEFESTRIIG+RQF+KA DLF+ ASE++ GKVDYRH YVDFSQLEVT+  +
Sbjct: 371 CYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQ 430

Query: 441 GGTTEVVQTCPAAMGFAFAAGTTDGPGAFDFEQGDDKGNPFWRFIRNLLKKPGKEQVDCQ 500
            G +EVV+TCPAAMGF FAAGTTDGPGAFDF+QGDD+GNPFWR +RNLLK P +EQV CQ
Sbjct: 431 NGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQ 490

Query: 501 QPKPILLDTGEMRKPYEWAPSILPIQILRVGQLIILGVPGEFTTMAGRRLRDAVKTVLTD 560
           +PKPILLDTGEM++PY+WAPSILP+QILR+GQL+IL VPGEFTTMAGRRLRDAVKTVL +
Sbjct: 491 RPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKE 550

Query: 561 GDKNGLSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLAC 620
           G  NG   +  +VIAGLTNSYSQY+ TFEEYQVQRYEGASTLYGPHTLS YIQEFKKLA 
Sbjct: 551 G-SNG--REFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLAN 610

Query: 621 ALIDGREVGAGPQPPDLLDKQIGLLPPVVMDTTPTSIKFGDIRSDVPQNSTFNRG-DMVT 680
            L+  +    GPQPPDLL KQI LL PVV D TP    FGD+ SDVP+ S F +G D+V 
Sbjct: 611 DLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADIVR 670

Query: 681 VTFWSACPRNDLMTEGTFALVE-ILHSKKLWVPAYDDDDFCLRFKWSRPFKLSSRSHATI 740
           V F SA PRNDLMTEGTFALVE  L  ++ WVP YDDDDFCLRFKWSRPFKLS++S ATI
Sbjct: 671 VQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATI 730

Query: 741 EWRIPKTAIPGMYRITHFGAAKSLFGSIHHFTGSSSPFGV 778
           EWRIP+TA PG+YRITHFG+AK+   SIHHF+GSSS F V
Sbjct: 731 EWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732

The following BLAST results are available for this feature:

BLAST of MS014232 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148376.1	0.0e+00	99.61	neutral ceramidase 1-like [Momordica charantia]	[more]
KAG6605432.1	0.0e+00	89.70	Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7029416.1	0.0e+00	89.70	Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023532163.1	0.0e+00	89.96	neutral ceramidase 1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_038901468.1	0.0e+00	89.45	neutral ceramidase 1 [Benincasa hispida]	[more]

BLAST of MS014232 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HQM3	0.0e+00	75.00	Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1	[more]
Q0JL46	0.0e+00	71.13	Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1...	[more]
Q304B9	0.0e+00	73.41	Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1	[more]
F4KHQ8	1.1e-309	70.53	Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1	[more]
Q55G11	8.6e-177	44.93	Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1	[more]

BLAST of MS014232 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D565	0.0e+00	99.61	Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111017041 PE=3 SV=1	[more]
A0A6J1G7Q7	0.0e+00	89.45	Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111451504 PE=3 SV=1	[more]
A0A6J1L802	0.0e+00	89.32	Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111500018 PE=3 SV=1	[more]
A0A5D3CV52	0.0e+00	89.07	Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G0...	[more]
A0A1S3CM50	0.0e+00	89.07	Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502048 PE=3 SV=1	[more]

BLAST of MS014232 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07380.1	0.0e+00	75.00	Neutral/alkaline non-lysosomal ceramidase	[more]
AT2G38010.1	0.0e+00	73.41	Neutral/alkaline non-lysosomal ceramidase	[more]
AT2G38010.2	0.0e+00	69.97	Neutral/alkaline non-lysosomal ceramidase	[more]
AT5G58980.1	7.9e-311	70.53	Neutral/alkaline non-lysosomal ceramidase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031331	Neutral/alkaline non-lysosomal ceramidase, C-terminal	PFAM	PF17048	Ceramidse_alk_C	coord: 614..777 e-value: 2.3E-50 score: 170.7
IPR038445	Neutral ceramidase, C-terminal domain superfamily	GENE3D	2.60.40.2300		coord: 645..778 e-value: 1.1E-44 score: 153.7
IPR031329	Neutral/alkaline non-lysosomal ceramidase, N-terminal	PFAM	PF04734	Ceramidase_alk	coord: 39..612 e-value: 9.5E-238 score: 789.8
IPR006823	Neutral/alkaline nonlysosomal ceramidase	PANTHER	PTHR12670	CERAMIDASE	coord: 18..777
None	No IPR available	PANTHER	PTHR12670:SF17	NEUTRAL CERAMIDASE 2	coord: 18..777

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014232.1	MS014232.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046514	ceramide catabolic process
molecular_function	GO:0017040	N-acylsphingosine amidohydrolase activity