MS014197 (gene) Bitter gourd (TR) v1

Overview
NameMS014197
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioninternal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like
Locationscaffold5: 1346154 .. 1348898 (+)
RNA-Seq ExpressionMS014197
SyntenyMS014197
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATGGTTCAGGAATCTCAGGCAACTTTCAGCGCTCAGATCCAGAAATCCGCGCCAAATTTCCCCCTCTACGACGCCGTTCGGCTTCTTCCCGCTTTCCCATTTCAGCTCGCAGTCGCCGCCGGCGGCTCGGCCCCCGGGGCTCGGGCCGACGGCGGCCGGCGAGAAGCCGAGGGTGGTGGTTCTCGGGTCGGGCTGGGCGGGTTGCCGAGTGATGAAGGGTTTGGACACCAGCATATACGACGTCGTTTGCGTGTCGCCGCGCAACCATATGGTCTTCACCCCTCTTCTGGCCTCAACCTGCGTGGGAACTCTCGAGTTCAGATCCGTGGCCGAACCCATCGCCCGGATCCAGCCCGCCATTTCTCGCGAACCCGGTTCGTACTTCTTCCTTGCGAATTGTACCGCCGTCGACACCGACGAACATTTGGTATGCTTTCTTTTGTAGCTTACTGTACTCAATGTTTCGTAGAAATTTGAAATTTCCGCTGAGTTTGGTGGGATGAGAGTGTCTGATTTCGTTAAGTTGGTTTCTGGAGGTGCAATGCGAGACTGTAACTGATGGATTGGACACTGTGGAGCCATGGAAGTTTAAGATATCGTATGATAAGTTGATTATAGCGTTGGGGTCGCAGCCACTGACTTTTGGGATTAATGGAGTCAAAGAGCACGCCATTTTTCTTCGTGAGGTTTATCATGCCCAGGAAATTCGCCGGAAGCTGCTTCTCAACTTGATGCTCTCTGATGTTCCAGGTATAGACTGTTTATTTTACTTGCGTTTCGGGTTTGGTAACTATTTCTTGTTACGGTGTCGGAAATGAGAAGAAGAAGCAAGAAACTAGATTTTATTGTTTTCGGAATCTCTACATCATATTTGGGAACTATTGTCAAAATGTTCATTGGGTGTTTCTGTTTGAGCCGTGGGGTCCGCTTGGTTTACCTATTGGCAAGTCGCATCCCCGTGGAAAAAATGATTTATTGGGAGGGCTATGAGAAATCCACTTTGTACTTTCGTTTCGTCATCAAAGCCTCTTGTCCCTGGGTCTTGGTTTACCCATCTTAATAAAGTGTATGATTGGCTCAAAAGAACATCTTACAATACAGGCGATTGGAGATAATATAACTAGCAAATATGTTCCAACATATTTTATTGTCAAGGAGGAATTACGGTTGCTTAGTAGTTACGGGATTTGCTATGACTTTTTCTACGTTTCTCAAAATTAGAACAAGAAACTAGATTTTGTTAATGTTTTTCTCCCTCAGTGAACATAGCTTGAATTTGTGTCATTGGTAGGCATCTCAGAGGAAGAGAAACTCCGGCTTCTACACTGTGTTGTGGTTGGAGGGGGACCAACTGGAGTTGAATTCAGTGGGGAGCTTAGTGATTTTATCAAGAGGGATGCTAGTCAAAGATATGCCCATGTGAAAAACTATATTCGAGTTACTCTGATTGAGGTTTGTAAATCCTCGATCGAAACACTGGATTCTTTATACATAATACACCGTGTCGTTTCCCCAAAGTTTCGGGGAAGTTGTCTCTCTATTCTATGTCGTTGAATACCTTTTTAAGACTTGAGAAGACTAACTTGCTTGTTTCTGTTTAATTGTTTAACAGGCAAATGAGATATTATCTTCCTTCGACGATCGCCTCCGGCACTATGCTACTAAACAATTGACTAAGGTAGGGAAATACATTAGAGTGATGCCTTTTTTCTCTTAGCGAGGATAGTAACAGGTTCCATATTTCTTCATTAGGATGCTTTAGATTTGAAATTCTTCGGTCTCATTTGTTCATATCCTCCAGTCAGGAGTTCAACTCGTACGTGGGATCGTCAAAGATGTTACTCCTCAGACCATCATTCTCGATGATGGCTCTAAGGTTCCTTATGGGTTGTTAGTTTGGTCTACTGGTGTTGGTCCCTCACCCTTTGTAAATTCCTTGGAAGTTCCAAAGTCTCCTGGTGGCAGGTAAGTTAAAATTTTATGCTTTCAGCTTGGCCCTTAGTTTCATTCAAATCATGGATTATGGTTTCCTATTTATGCTTCATTTTCTATTAGTAATCAAACATTGCATAGAGAGAGATGACGAGAACGTTTTATCTCTGATTATGTTGTCAGGATCGGTATAGATGAATGGTTACGTGTTCCTTCTGTGGAAGATGTGTTTGCAATAGGCGATTGCAGCGGGTTCCTAGAGAGTACAGGGAAACCGGCTCTTCCAGCTTTGGCCCAGGTAAGTTGAGATACTTCTGAATATTGTGGTTTTTCTGCTGTTGCCAAGAGATGTCAAGTTTTGTATTGCAACCATTTTTTGCATTCTGAAGGTAGCAGAGCGCCAAGGCAAATATCTTGCAGCCCTGCTGAATAAGATTGGTAGAGAAGGTGGGGCACGAGCGGGTAGCGCGAAAGAGTTTGAACTCGGAGCGCCATTTGTCTACAAGCACCTAGGAAGTATGGCAACTATTGGAAGATACAAGGCTCTGGTCGACCTTAGGCAGAGCAAAGTAAGCAACAGATCGAGTTGCTCCGGGGTTCCTTTCTTTCTTGCTCTATATCGTGAATTGGGTGGCTTACTTGTGATAATTTTCTACAGGAAGCAAAGGGGATAGCCATGGCAGGGTTCCTCAGTTGGTTCGTTTGGCGTTCGGCTTATCTAACGCGGGTCATAAGCTGGAGGAATAGATTGTATGTTGCAGTGAACTGGGCAACAACGTTTATCTTCGGTCGCGATATAAGCAGAATA

mRNA sequence

ATGACATGGTTCAGGAATCTCAGGCAACTTTCAGCGCTCAGATCCAGAAATCCGCGCCAAATTTCCCCCTCTACGACGCCGTTCGGCTTCTTCCCGCTTTCCCATTTCAGCTCGCAGTCGCCGCCGGCGGCTCGGCCCCCGGGGCTCGGGCCGACGGCGGCCGGCGAGAAGCCGAGGGTGGTGGTTCTCGGGTCGGGCTGGGCGGGTTGCCGAGTGATGAAGGGTTTGGACACCAGCATATACGACGTCGTTTGCGTGTCGCCGCGCAACCATATGGTCTTCACCCCTCTTCTGGCCTCAACCTGCGTGGGAACTCTCGAGTTCAGATCCGTGGCCGAACCCATCGCCCGGATCCAGCCCGCCATTTCTCGCGAACCCGGTTCGTACTTCTTCCTTGCGAATTGTACCGCCGTCGACACCGACGAACATTTGGTGCAATGCGAGACTGTAACTGATGGATTGGACACTGTGGAGCCATGGAAGTTTAAGATATCGTATGATAAGTTGATTATAGCGTTGGGGTCGCAGCCACTGACTTTTGGGATTAATGGAGTCAAAGAGCACGCCATTTTTCTTCGTGAGGTTTATCATGCCCAGGAAATTCGCCGGAAGCTGCTTCTCAACTTGATGCTCTCTGATGTTCCAGGCATCTCAGAGGAAGAGAAACTCCGGCTTCTACACTGTGTTGTGGTTGGAGGGGGACCAACTGGAGTTGAATTCAGTGGGGAGCTTAGTGATTTTATCAAGAGGGATGCTAGTCAAAGATATGCCCATGTGAAAAACTATATTCGAGTTACTCTGATTGAGGCAAATGAGATATTATCTTCCTTCGACGATCGCCTCCGGCACTATGCTACTAAACAATTGACTAAGTCAGGAGTTCAACTCGTACGTGGGATCGTCAAAGATGTTACTCCTCAGACCATCATTCTCGATGATGGCTCTAAGGTTCCTTATGGGTTGTTAGTTTGGTCTACTGGTGTTGGTCCCTCACCCTTTGTAAATTCCTTGGAAGTTCCAAAGTCTCCTGGTGGCAGGATCGGTATAGATGAATGGTTACGTGTTCCTTCTGTGGAAGATGTGTTTGCAATAGGCGATTGCAGCGGGTTCCTAGAGAGTACAGGGAAACCGGCTCTTCCAGCTTTGGCCCAGGTAGCAGAGCGCCAAGGCAAATATCTTGCAGCCCTGCTGAATAAGATTGGTAGAGAAGGTGGGGCACGAGCGGGTAGCGCGAAAGAGTTTGAACTCGGAGCGCCATTTGTCTACAAGCACCTAGGAAGTATGGCAACTATTGGAAGATACAAGGCTCTGGTCGACCTTAGGCAGAGCAAAGAAGCAAAGGGGATAGCCATGGCAGGGTTCCTCAGTTGGTTCGTTTGGCGTTCGGCTTATCTAACGCGGGTCATAAGCTGGAGGAATAGATTGTATGTTGCAGTGAACTGGGCAACAACGTTTATCTTCGGTCGCGATATAAGCAGAATA

Coding sequence (CDS)

ATGACATGGTTCAGGAATCTCAGGCAACTTTCAGCGCTCAGATCCAGAAATCCGCGCCAAATTTCCCCCTCTACGACGCCGTTCGGCTTCTTCCCGCTTTCCCATTTCAGCTCGCAGTCGCCGCCGGCGGCTCGGCCCCCGGGGCTCGGGCCGACGGCGGCCGGCGAGAAGCCGAGGGTGGTGGTTCTCGGGTCGGGCTGGGCGGGTTGCCGAGTGATGAAGGGTTTGGACACCAGCATATACGACGTCGTTTGCGTGTCGCCGCGCAACCATATGGTCTTCACCCCTCTTCTGGCCTCAACCTGCGTGGGAACTCTCGAGTTCAGATCCGTGGCCGAACCCATCGCCCGGATCCAGCCCGCCATTTCTCGCGAACCCGGTTCGTACTTCTTCCTTGCGAATTGTACCGCCGTCGACACCGACGAACATTTGGTGCAATGCGAGACTGTAACTGATGGATTGGACACTGTGGAGCCATGGAAGTTTAAGATATCGTATGATAAGTTGATTATAGCGTTGGGGTCGCAGCCACTGACTTTTGGGATTAATGGAGTCAAAGAGCACGCCATTTTTCTTCGTGAGGTTTATCATGCCCAGGAAATTCGCCGGAAGCTGCTTCTCAACTTGATGCTCTCTGATGTTCCAGGCATCTCAGAGGAAGAGAAACTCCGGCTTCTACACTGTGTTGTGGTTGGAGGGGGACCAACTGGAGTTGAATTCAGTGGGGAGCTTAGTGATTTTATCAAGAGGGATGCTAGTCAAAGATATGCCCATGTGAAAAACTATATTCGAGTTACTCTGATTGAGGCAAATGAGATATTATCTTCCTTCGACGATCGCCTCCGGCACTATGCTACTAAACAATTGACTAAGTCAGGAGTTCAACTCGTACGTGGGATCGTCAAAGATGTTACTCCTCAGACCATCATTCTCGATGATGGCTCTAAGGTTCCTTATGGGTTGTTAGTTTGGTCTACTGGTGTTGGTCCCTCACCCTTTGTAAATTCCTTGGAAGTTCCAAAGTCTCCTGGTGGCAGGATCGGTATAGATGAATGGTTACGTGTTCCTTCTGTGGAAGATGTGTTTGCAATAGGCGATTGCAGCGGGTTCCTAGAGAGTACAGGGAAACCGGCTCTTCCAGCTTTGGCCCAGGTAGCAGAGCGCCAAGGCAAATATCTTGCAGCCCTGCTGAATAAGATTGGTAGAGAAGGTGGGGCACGAGCGGGTAGCGCGAAAGAGTTTGAACTCGGAGCGCCATTTGTCTACAAGCACCTAGGAAGTATGGCAACTATTGGAAGATACAAGGCTCTGGTCGACCTTAGGCAGAGCAAAGAAGCAAAGGGGATAGCCATGGCAGGGTTCCTCAGTTGGTTCGTTTGGCGTTCGGCTTATCTAACGCGGGTCATAAGCTGGAGGAATAGATTGTATGTTGCAGTGAACTGGGCAACAACGTTTATCTTCGGTCGCGATATAAGCAGAATA

Protein sequence

MTWFRNLRQLSALRSRNPRQISPSTTPFGFFPLSHFSSQSPPAARPPGLGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYVAVNWATTFIFGRDISRI
Homology
BLAST of MS014197 vs. NCBI nr
Match: XP_023512652.1 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894.8 bits (2311), Expect = 3.3e-256
Identity = 444/501 (88.62%), Postives = 469/501 (93.61%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR  NP    PST PF F  LSHFSSQ+      ARPPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFTNPHPFFPSTAPFAFL-LSHFSSQASSDAVVARPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQPAISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPAISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARAGS K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARAGSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           RLYVAVNWATTFIFGRDISRI
Sbjct: 481 RLYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. NCBI nr
Match: XP_022944050.1 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 442/501 (88.22%), Postives = 469/501 (93.61%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR  NP    PST PF F  LSHFSSQ+      ARPPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFTNPHPFFPSTAPFAFL-LSHFSSQASSDAVVARPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQP+ISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPSISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARAGS K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARAGSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKE+KG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKESKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           RLYVAVNWATTFIFGRDISRI
Sbjct: 481 RLYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. NCBI nr
Match: XP_022986852.1 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 892.1 bits (2304), Expect = 2.2e-255
Identity = 442/501 (88.22%), Postives = 469/501 (93.61%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR +NP    PST PF F  LSHFSSQ+      A+PPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFKNPHPFFPSTAPFAFL-LSHFSSQASSDAVVAQPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQPAISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPAISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARA S K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARASSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           RLYVAVNWATTFIFGRDISRI
Sbjct: 481 RLYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. NCBI nr
Match: KAG7010500.1 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 890.2 bits (2299), Expect = 8.2e-255
Identity = 440/501 (87.82%), Postives = 468/501 (93.41%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR  NP    PST PF F  LSHFSSQ+      ARPPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFTNPHPFFPSTAPFAFL-LSHFSSQASSDAVVARPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQP+ISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPSISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VP+GLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPFGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARAGS K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARAGSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPF+YKHLGSMATIGRYKALVDLRQSKEAKG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFIYKHLGSMATIGRYKALVDLRQSKEAKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           R YVAVNWATTFIFGRDISRI
Sbjct: 481 RFYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. NCBI nr
Match: KAG6570650.1 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 886.7 bits (2290), Expect = 9.1e-254
Identity = 439/501 (87.62%), Postives = 466/501 (93.01%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR  NP    PST PF    LSHFSSQ+      ARPPGLGPT 
Sbjct: 1   MAWFRNLKQLSSTKASLRFTNPHPFFPSTAPFALL-LSHFSSQASSDAVVARPPGLGPTV 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQP+ISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPSISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VP+GLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPFGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARAGS K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARAGSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           R YVAVNWATTFIFGRDISRI
Sbjct: 481 RFYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. ExPASy Swiss-Prot
Match: Q8GWA1 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDA1 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.3e-218
Identity = 364/450 (80.89%), Postives = 405/450 (90.00%), Query Frame = 0

Query: 45  RPPGLGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVG 104
           R  GL PT  GEKPRV+VLGSGWAGCRV+KG+DTSIYDVVCVSPRNHMVFTPLLASTCVG
Sbjct: 61  RYDGLAPTKEGEKPRVLVLGSGWAGCRVLKGIDTSIYDVVCVSPRNHMVFTPLLASTCVG 120

Query: 105 TLEFRSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKI 164
           TLEFRSVAEPI+RIQPAISREPGSY+FLANC+ +D D H V CETVT+G  T++PWKFKI
Sbjct: 121 TLEFRSVAEPISRIQPAISREPGSYYFLANCSKLDADNHEVHCETVTEGSSTLKPWKFKI 180

Query: 165 SYDKLIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLR 224
           +YDKL++A G++  TFGINGV E+AIFLREV+HAQEIRRKLLLNLMLS+VPGI E+EK R
Sbjct: 181 AYDKLVLACGAEASTFGINGVLENAIFLREVHHAQEIRRKLLLNLMLSEVPGIGEDEKKR 240

Query: 225 LLHCVVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHY 284
           LLHCVVVGGGPTGVEFSGELSDFI +D  QRY+HVK+ IRVTLIEA +ILSSFDDRLRHY
Sbjct: 241 LLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYSHVKDDIRVTLIEARDILSSFDDRLRHY 300

Query: 285 ATKQLTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPG 344
           A KQL KSGV+LVRGIVK+V PQ +ILDDG++VPYG LVWSTGVGPS FV SL+ PK PG
Sbjct: 301 AIKQLNKSGVKLVRGIVKEVKPQKLILDDGTEVPYGPLVWSTGVGPSSFVRSLDFPKDPG 360

Query: 345 GRIGIDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREG 404
           GRIGIDEW+RVPSV+DVFAIGDCSG+LESTGK  LPALAQVAER+GKYLA L N +G+ G
Sbjct: 361 GRIGIDEWMRVPSVQDVFAIGDCSGYLESTGKSTLPALAQVAEREGKYLANLFNVMGKAG 420

Query: 405 GARAGSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAY 464
           G RA SAKE ELG PFVYKHLGSMATIGRYKALVDLR+SKE KGI+MAGFLSWF+WRSAY
Sbjct: 421 GGRANSAKEMELGEPFVYKHLGSMATIGRYKALVDLRESKEGKGISMAGFLSWFIWRSAY 480

Query: 465 LTRVISWRNRLYVAVNWATTFIFGRDISRI 495
           LTRV+SWRNR YVA+NW TTF+FGRDISRI
Sbjct: 481 LTRVVSWRNRFYVAINWLTTFVFGRDISRI 510

BLAST of MS014197 vs. ExPASy Swiss-Prot
Match: O80874 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDA2 PE=1 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 7.1e-217
Identity = 377/508 (74.21%), Postives = 423/508 (83.27%), Query Frame = 0

Query: 1   MTWFRNLRQLS------ALRSRNPRQISPSTTPFGFFPLSHFSSQSPPAARP-------- 60
           M   +NL ++S        R RN    S S T    F  +  +    PA  P        
Sbjct: 1   MFMIKNLTRISPNTSSIITRFRNSGSSSLSYTLASRFCTAQETQIQSPAKIPNDVDRSQY 60

Query: 61  PGLGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTL 120
            GL PT  GEKPRVVVLGSGWAGCR+MKG+DT++YDVVCVSPRNHMVFTPLLASTCVGTL
Sbjct: 61  SGLPPTREGEKPRVVVLGSGWAGCRLMKGIDTNLYDVVCVSPRNHMVFTPLLASTCVGTL 120

Query: 121 EFRSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISY 180
           EFRSVAEPI+RIQPAISREPGS+FFLANC+ +D D H V CET+TDGL+T++PWKFKI+Y
Sbjct: 121 EFRSVAEPISRIQPAISREPGSFFFLANCSRLDADAHEVHCETLTDGLNTLKPWKFKIAY 180

Query: 181 DKLIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLL 240
           DKL+IA G++  TFGI+GV E+AIFLREV+HAQEIRRKLLLNLMLSD PGIS+EEK RLL
Sbjct: 181 DKLVIASGAEASTFGIHGVMENAIFLREVHHAQEIRRKLLLNLMLSDTPGISKEEKRRLL 240

Query: 241 HCVVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYAT 300
           HCVVVGGGPTGVEFSGELSDFI +D  QRYAHVK+ I VTLIEA +ILSSFDDRLR YA 
Sbjct: 241 HCVVVGGGPTGVEFSGELSDFIMKDVRQRYAHVKDDIHVTLIEARDILSSFDDRLRRYAI 300

Query: 301 KQLTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGR 360
           KQL KSGV+ VRGIVKDV  Q +ILDDG++VPYGLLVWSTGVGPSPFV SL +PK P GR
Sbjct: 301 KQLNKSGVRFVRGIVKDVQSQKLILDDGTEVPYGLLVWSTGVGPSPFVRSLGLPKDPTGR 360

Query: 361 IGIDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGA 420
           IGIDEW+RVPSV+DVFAIGDCSG+LE+TGKP LPALAQVAER+GKYLA LLN IG+  G 
Sbjct: 361 IGIDEWMRVPSVQDVFAIGDCSGYLETTGKPTLPALAQVAEREGKYLANLLNAIGKGNGG 420

Query: 421 RAGSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLT 480
           RA SAKE ELG PFVYKHLGSMATIGRYKALVDLR+SK+AKGI+M GF+SWF+WRSAYLT
Sbjct: 421 RANSAKEIELGVPFVYKHLGSMATIGRYKALVDLRESKDAKGISMTGFVSWFIWRSAYLT 480

Query: 481 RVISWRNRLYVAVNWATTFIFGRDISRI 495
           RVISWRNR YVA+NW TTF+FGRDISRI
Sbjct: 481 RVISWRNRFYVAINWFTTFVFGRDISRI 508

BLAST of MS014197 vs. ExPASy Swiss-Prot
Match: M0ZYF3 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDA1 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 3.0e-215
Identity = 364/497 (73.24%), Postives = 421/497 (84.71%), Query Frame = 0

Query: 1   MTWFRNLRQLSAL---RSRNPRQISPSTTPFGFFPLSHFSSQSPPAARPPGLGPTAAGEK 60
           M WF+NL ++S     +S + + I+P  +P        F+ Q        GL  T + +K
Sbjct: 1   MPWFKNLIKISKTITNQSSSYKSITPLASPL-LAQFLQFTKQYSTNDHVVGLEATKSDQK 60

Query: 61  PRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIAR 120
           PR+VVLGSGWAGCR+MK +DT+IYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI R
Sbjct: 61  PRIVVLGSGWAGCRLMKDIDTNIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIGR 120

Query: 121 IQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQP 180
           IQPA+S +P SYFFLANC A+D D H++QC+TVT+G++T+EPWKF +SYDKL+IA G+  
Sbjct: 121 IQPAVSTQPASYFFLANCNAIDFDNHMIQCQTVTEGVETLEPWKFNVSYDKLVIASGAHA 180

Query: 181 LTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTG 240
           LTFGI GV EHA FLREV+HAQEIRRKLLLNLMLSDVPG+SEEEK RLLHCVVVGGGPTG
Sbjct: 181 LTFGIKGVNEHATFLREVHHAQEIRRKLLLNLMLSDVPGVSEEEKRRLLHCVVVGGGPTG 240

Query: 241 VEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLV 300
           VEFSGELSDFI +D  QRYAHVK+YI VTLIEANEILSSFDDRLR YAT QLTKSGV+LV
Sbjct: 241 VEFSGELSDFILKDVHQRYAHVKDYIHVTLIEANEILSSFDDRLRVYATNQLTKSGVRLV 300

Query: 301 RGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPS 360
           RG+V+ V P  IIL DG+ VPYGLLVWSTGVGPSPFVNSL++PK+  GRIGIDEWLRVPS
Sbjct: 301 RGLVQHVQPDKIILSDGTNVPYGLLVWSTGVGPSPFVNSLDIPKAK-GRIGIDEWLRVPS 360

Query: 361 VEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELG 420
           V+DV++IGDCSGFLESTG+  LPALAQVAERQGKYLA+LLNK+G+EGG  A  A+   LG
Sbjct: 361 VQDVYSIGDCSGFLESTGRQVLPALAQVAERQGKYLASLLNKVGKEGGGHANCAQNINLG 420

Query: 421 APFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYV 480
            PFVYKHLGSMATIGRYKALVDLR+SKEAKG+++AGF S+FVWRSAYLTRV+SWRN++YV
Sbjct: 421 DPFVYKHLGSMATIGRYKALVDLRESKEAKGVSLAGFTSFFVWRSAYLTRVVSWRNKIYV 480

Query: 481 AVNWATTFIFGRDISRI 495
            +NW TT +FGRDISRI
Sbjct: 481 LINWLTTLVFGRDISRI 495

BLAST of MS014197 vs. ExPASy Swiss-Prot
Match: Q9ST63 (Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDA1 PE=1 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 4.3e-214
Identity = 362/497 (72.84%), Postives = 420/497 (84.51%), Query Frame = 0

Query: 1   MTWFRNLRQLSAL---RSRNPRQISPSTTPFGFFPLSHFSSQSPPAARPPGLGPTAAGEK 60
           M WF+NL ++S     +S + + I+P  +P        F+ Q        GL  T + +K
Sbjct: 1   MPWFKNLIKISKTITNQSSSYKSITPLASPL-LTQFLQFTKQYSTNDHVVGLEATKSDQK 60

Query: 61  PRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIAR 120
           PR+VVLGSGWAGCR+MK +DT+IYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI R
Sbjct: 61  PRIVVLGSGWAGCRLMKDIDTNIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIGR 120

Query: 121 IQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQP 180
           IQPA+S +P SYFFLANC A+D D H+++CETVT+G++T+E WKF +SYDKL+IA G+  
Sbjct: 121 IQPAVSTQPASYFFLANCNAIDFDNHMIECETVTEGVETLEAWKFNVSYDKLVIASGAHA 180

Query: 181 LTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTG 240
           LTFGI GV EHA FLREV+HAQEIRRKLLLNLMLSDVPG+SEEEK RLLHCVVVGGGPTG
Sbjct: 181 LTFGIKGVNEHATFLREVHHAQEIRRKLLLNLMLSDVPGVSEEEKRRLLHCVVVGGGPTG 240

Query: 241 VEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLV 300
           VEFSGELSDFI +D  QRYAHVK+YI VTLIEANEILSSFDDRLR YAT QLTKSGV+LV
Sbjct: 241 VEFSGELSDFILKDVHQRYAHVKDYIHVTLIEANEILSSFDDRLRVYATNQLTKSGVRLV 300

Query: 301 RGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPS 360
           RG+V+ V P  IIL DG+ VPYGLLVWSTGVGPSPFVNSL++PK+  GRIGIDEWLRVPS
Sbjct: 301 RGLVQHVQPDNIILSDGTNVPYGLLVWSTGVGPSPFVNSLDIPKAK-GRIGIDEWLRVPS 360

Query: 361 VEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELG 420
           V+DV++IGDCSGFLESTG+  LPALAQVAERQGKYLA+LLNK+G++GG  A  A+   LG
Sbjct: 361 VQDVYSIGDCSGFLESTGRQVLPALAQVAERQGKYLASLLNKVGKQGGGHANCAQNINLG 420

Query: 421 APFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYV 480
            PFVYKHLGSMATIGRYKALVDLR+SKEAKG+++AGF S+FVWRSAYLTRV+SWRN++YV
Sbjct: 421 DPFVYKHLGSMATIGRYKALVDLRESKEAKGVSLAGFTSFFVWRSAYLTRVVSWRNKIYV 480

Query: 481 AVNWATTFIFGRDISRI 495
            +NW TT +FGRDISRI
Sbjct: 481 LINWLTTLVFGRDISRI 495

BLAST of MS014197 vs. ExPASy Swiss-Prot
Match: Q55CD9 (Probable NADH dehydrogenase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0270104 PE=3 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 1.1e-79
Identity = 169/441 (38.32%), Postives = 260/441 (58.96%), Query Frame = 0

Query: 56  EKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI 115
           E  ++++LG GW     +K L++  YD+  +SPRNH +FTPLL S+ VGTLEFRS+AEP+
Sbjct: 38  ENEKLIILGCGWGSYSFLKNLNSIKYDITVISPRNHFLFTPLLTSSAVGTLEFRSIAEPV 97

Query: 116 ARIQPAISREPGSYFFL-ANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALG 175
                  +R+   + ++ A+ T+++ + + V    V       +P  F++ YDKL+I +G
Sbjct: 98  -----RTTRDINEFKYIQASVTSINPENNSV---LVKSTFHNEKP--FEMKYDKLVIGVG 157

Query: 176 SQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGG 235
           S+  TFGI GV+E+A FL+E++HA+EIR+K++     + +P +S EE+ RLL  V+VGGG
Sbjct: 158 SRNNTFGIKGVEENANFLKELHHAREIRQKIIECFERASLPDVSTEERERLLSFVIVGGG 217

Query: 236 PTGVEFSGELSDFIKRDASQRYAHVK-NYIRVTLIEAN-EILSSFDDRLRHYATKQLTKS 295
            TG+EF+ EL+DF   D S+ +  V  N +++ L+EA+ +ILS+FD +L   A      S
Sbjct: 218 ATGIEFTSELNDFFSEDLSRLFPFVPVNEVKIILLEASGKILSTFDQKLVKKALINFRNS 277

Query: 296 GVQL-VRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDE 355
           G+ +     VK+V    +ILD+G ++PYGLLVWSTG+G  P V +    K    RI +D+
Sbjct: 278 GIDVRTHSSVKEVLKDYVILDNGDRIPYGLLVWSTGIGQHPLVKNSSFEKDSHDRIIVDD 337

Query: 356 WLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSA 415
            LRV +  +VF+ GDC+    +      P  AQVA +   YLA   N + +         
Sbjct: 338 HLRVKNYSNVFSFGDCA----NVENKNYPPTAQVASQSAVYLAKEFNNLEK---LNPNPP 397

Query: 416 KEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISW 475
           K      PF +K LG +A  G+   ++      +     ++GF+ +  WRSAYLTR+ S 
Sbjct: 398 K------PFAFKFLGLLAYTGKKSGIL------QTDFFDLSGFIGFITWRSAYLTRLGSL 449

Query: 476 RNRLYVAVNWATTFIFGRDIS 493
           R+++ V  +W  T IFGRDIS
Sbjct: 458 RSKIQVPFDWMRTLIFGRDIS 449

BLAST of MS014197 vs. ExPASy TrEMBL
Match: A0A6J1FYF8 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448601 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.0e-256
Identity = 442/501 (88.22%), Postives = 469/501 (93.61%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR  NP    PST PF F  LSHFSSQ+      ARPPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFTNPHPFFPSTAPFAFL-LSHFSSQASSDAVVARPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQP+ISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPSISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARAGS K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARAGSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKE+KG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKESKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           RLYVAVNWATTFIFGRDISRI
Sbjct: 481 RLYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. ExPASy TrEMBL
Match: A0A6J1J8Q2 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111484468 PE=4 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.0e-255
Identity = 442/501 (88.22%), Postives = 469/501 (93.61%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPP---AARPPGLGPTA 60
           M WFRNL+QLS    +LR +NP    PST PF F  LSHFSSQ+      A+PPGLGPTA
Sbjct: 1   MAWFRNLKQLSSTKASLRFKNPHPFFPSTAPFAFL-LSHFSSQASSDAVVAQPPGLGPTA 60

Query: 61  AGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120
            GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE
Sbjct: 61  EGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAE 120

Query: 121 PIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIAL 180
           PI RIQPAISREPGSYFFLANCT V+TDEH+VQCETVTDGL+T+EPW+FK+SYDKLIIAL
Sbjct: 121 PIGRIQPAISREPGSYFFLANCTGVNTDEHVVQCETVTDGLNTLEPWRFKLSYDKLIIAL 180

Query: 181 GSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGG 240
           GSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEK RLLHCVVVGG
Sbjct: 181 GSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGG 240

Query: 241 GPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSG 300
           GPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQLTKSG
Sbjct: 241 GPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQLTKSG 300

Query: 301 VQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWL 360
           VQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSL+VPKSPGGRIGIDEWL
Sbjct: 301 VQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLDVPKSPGGRIGIDEWL 360

Query: 361 RVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKE 420
           RVPSVEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAAL+NKIG+EGGARA S K+
Sbjct: 361 RVPSVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALMNKIGKEGGARASSGKD 420

Query: 421 FELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRN 480
            ELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKG++MAGF+SWFVWRSAYLTRVISWRN
Sbjct: 421 LELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGLSMAGFISWFVWRSAYLTRVISWRN 480

Query: 481 RLYVAVNWATTFIFGRDISRI 495
           RLYVAVNWATTFIFGRDISRI
Sbjct: 481 RLYVAVNWATTFIFGRDISRI 500

BLAST of MS014197 vs. ExPASy TrEMBL
Match: A0A1S3CKL1 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502015 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.3e-250
Identity = 439/506 (86.76%), Postives = 463/506 (91.50%), Query Frame = 0

Query: 1   MTWFRNLRQLS----ALRSRNPRQISPSTTPFGFFPLSHFSSQSPPAA--------RPPG 60
           M WFRNL++LS     LRS NP    PST     F LSHFSS   P A        RPPG
Sbjct: 3   MAWFRNLKKLSLTKPPLRSTNPHPFLPST-----FLLSHFSSHPIPDAAAEAAELSRPPG 62

Query: 61  LGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEF 120
           LGPT +GEKPRVVVLGSGWAGCR+MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEF
Sbjct: 63  LGPTVSGEKPRVVVLGSGWAGCRLMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEF 122

Query: 121 RSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDK 180
           RSVAEPI RIQP+ISREPGSYFFLANCT+V+TDEH VQCETVTDGL+T++PW+FK+SYDK
Sbjct: 123 RSVAEPIGRIQPSISREPGSYFFLANCTSVNTDEHSVQCETVTDGLNTLKPWRFKLSYDK 182

Query: 181 LIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHC 240
           LIIALGSQPLTFGI+GVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGIS EEK RLLHC
Sbjct: 183 LIIALGSQPLTFGIHGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISVEEKRRLLHC 242

Query: 241 VVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQ 300
           VVVGGGPTGVEFSGELSDFI +D +QRYAHVK+YI+VTLIEANEILSSFDDRLRHYATKQ
Sbjct: 243 VVVGGGPTGVEFSGELSDFIIKDVTQRYAHVKDYIQVTLIEANEILSSFDDRLRHYATKQ 302

Query: 301 LTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIG 360
           LTKSGVQLVRGIVKDV PQ+IIL+DGS+VPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIG
Sbjct: 303 LTKSGVQLVRGIVKDVKPQSIILNDGSEVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIG 362

Query: 361 IDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARA 420
           IDEWLRVP+VEDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLAALLNKIG+EGG RA
Sbjct: 363 IDEWLRVPAVEDVFAIGDCSGFLESTGKQVLPALAQVAERQGKYLAALLNKIGKEGGGRA 422

Query: 421 GSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRV 480
           GS K+ ELG PFVYKHLGSMATIGRYKALVDLRQSKEAKGI+MAGFLSWFVWRSAYLTRV
Sbjct: 423 GSGKDLELGDPFVYKHLGSMATIGRYKALVDLRQSKEAKGISMAGFLSWFVWRSAYLTRV 482

Query: 481 ISWRNRLYVAVNWATTFIFGRDISRI 495
           ISWRNRLYVAVNWATTFIFGRDISRI
Sbjct: 483 ISWRNRLYVAVNWATTFIFGRDISRI 503

BLAST of MS014197 vs. ExPASy TrEMBL
Match: A0A6J1D519 (internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111017079 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.0e-243
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 73  MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIARIQPAISREPGSYFFL 132
           MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIARIQPAISREPGSYFFL
Sbjct: 1   MKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIARIQPAISREPGSYFFL 60

Query: 133 ANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQPLTFGINGVKEHAIFL 192
           ANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQPLTFGINGVKEHAIFL
Sbjct: 61  ANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQPLTFGINGVKEHAIFL 120

Query: 193 REVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTGVEFSGELSDFIKRDA 252
           REVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTGVEFSGELSDFIKRDA
Sbjct: 121 REVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTGVEFSGELSDFIKRDA 180

Query: 253 SQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLVRGIVKDVTPQTIILD 312
           SQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLVRGIVKDVTPQTIILD
Sbjct: 181 SQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLVRGIVKDVTPQTIILD 240

Query: 313 DGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPSVEDVFAIGDCSGFLE 372
           DGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPSVEDVFAIGDCSGFLE
Sbjct: 241 DGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPSVEDVFAIGDCSGFLE 300

Query: 373 STGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELGAPFVYKHLGSMATIG 432
           STGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELGAPFVYKHLGSMATIG
Sbjct: 301 STGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELGAPFVYKHLGSMATIG 360

Query: 433 RYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYVAVNWATTFIFGRDIS 492
           RYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYVAVNWATTFIFGRDIS
Sbjct: 361 RYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYVAVNWATTFIFGRDIS 420

Query: 493 RI 495
           RI
Sbjct: 421 RI 422

BLAST of MS014197 vs. ExPASy TrEMBL
Match: A0A6J1L3V7 (internal alternative NAD(P)H-ubiquinone oxidoreductase A2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111499652 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.0e-239
Identity = 420/497 (84.51%), Postives = 445/497 (89.54%), Query Frame = 0

Query: 1   MTWFRNLRQLSALRSRNPRQISP---STTPFGFFPLSHFSSQSPPAARPPGLGPTAAGEK 60
           M WFRNLRQLS      P  I P   STTP   F LSHFSSQ      PPGL PT  G+K
Sbjct: 1   MAWFRNLRQLS------PTTIPPPPKSTTPSFTFLLSHFSSQPISKVPPPGLSPTVPGQK 60

Query: 61  PRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIAR 120
           PRVVVLGSGW GCR+MKGLDT++YDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI R
Sbjct: 61  PRVVVLGSGWGGCRLMKGLDTTVYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIVR 120

Query: 121 IQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQP 180
           IQPAISREPGSYFFLANCT +  +EH VQCETVTDGL+T EP +FKISYDKLIIALGSQP
Sbjct: 121 IQPAISREPGSYFFLANCTGIKPEEHSVQCETVTDGLNTSEPRRFKISYDKLIIALGSQP 180

Query: 181 LTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPTG 240
           LTFGI+GVKEHA FLREV HAQEIRRKLLLNLMLSDVPGIS +EK RLLHCVVVGGGPTG
Sbjct: 181 LTFGIHGVKEHAFFLREVSHAQEIRRKLLLNLMLSDVPGISVQEKRRLLHCVVVGGGPTG 240

Query: 241 VEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYATKQLTKSGVQLV 300
           VEFSGEL DFI +D SQRYAHVK+ I+VTLIEANEILSSFDDRL+ YAT QLTKSGVQ+V
Sbjct: 241 VEFSGELYDFIIKDVSQRYAHVKDCIQVTLIEANEILSSFDDRLQRYATNQLTKSGVQIV 300

Query: 301 RGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPS 360
           +GIVKDV PQTIIL DGS++PYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPS
Sbjct: 301 QGIVKDVKPQTIILKDGSEIPYGLLVWSTGVGPSPFVNSLEVPKSPGGRIGIDEWLRVPS 360

Query: 361 VEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGARAGSAKEFELG 420
            EDVFAIGDCSGFLESTGK  LPALAQVAERQGKYLA LLNK+G+EGGARAGSAK+ +LG
Sbjct: 361 AEDVFAIGDCSGFLESTGKQILPALAQVAERQGKYLATLLNKVGKEGGARAGSAKDLDLG 420

Query: 421 APFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLTRVISWRNRLYV 480
           APFVYKHLGSMATIGRYKALVDLRQSK AKG++MAGF+SWFVWRSAYLTRVISWRNRLYV
Sbjct: 421 APFVYKHLGSMATIGRYKALVDLRQSKAAKGLSMAGFVSWFVWRSAYLTRVISWRNRLYV 480

Query: 481 AVNWATTFIFGRDISRI 495
           AVNWATTFIFGRDISRI
Sbjct: 481 AVNWATTFIFGRDISRI 491

BLAST of MS014197 vs. TAIR 10
Match: AT1G07180.1 (alternative NAD(P)H dehydrogenase 1 )

HSP 1 Score: 760.4 bits (1962), Expect = 9.1e-220
Identity = 364/450 (80.89%), Postives = 405/450 (90.00%), Query Frame = 0

Query: 45  RPPGLGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVG 104
           R  GL PT  GEKPRV+VLGSGWAGCRV+KG+DTSIYDVVCVSPRNHMVFTPLLASTCVG
Sbjct: 61  RYDGLAPTKEGEKPRVLVLGSGWAGCRVLKGIDTSIYDVVCVSPRNHMVFTPLLASTCVG 120

Query: 105 TLEFRSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKI 164
           TLEFRSVAEPI+RIQPAISREPGSY+FLANC+ +D D H V CETVT+G  T++PWKFKI
Sbjct: 121 TLEFRSVAEPISRIQPAISREPGSYYFLANCSKLDADNHEVHCETVTEGSSTLKPWKFKI 180

Query: 165 SYDKLIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLR 224
           +YDKL++A G++  TFGINGV E+AIFLREV+HAQEIRRKLLLNLMLS+VPGI E+EK R
Sbjct: 181 AYDKLVLACGAEASTFGINGVLENAIFLREVHHAQEIRRKLLLNLMLSEVPGIGEDEKKR 240

Query: 225 LLHCVVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHY 284
           LLHCVVVGGGPTGVEFSGELSDFI +D  QRY+HVK+ IRVTLIEA +ILSSFDDRLRHY
Sbjct: 241 LLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYSHVKDDIRVTLIEARDILSSFDDRLRHY 300

Query: 285 ATKQLTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPG 344
           A KQL KSGV+LVRGIVK+V PQ +ILDDG++VPYG LVWSTGVGPS FV SL+ PK PG
Sbjct: 301 AIKQLNKSGVKLVRGIVKEVKPQKLILDDGTEVPYGPLVWSTGVGPSSFVRSLDFPKDPG 360

Query: 345 GRIGIDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREG 404
           GRIGIDEW+RVPSV+DVFAIGDCSG+LESTGK  LPALAQVAER+GKYLA L N +G+ G
Sbjct: 361 GRIGIDEWMRVPSVQDVFAIGDCSGYLESTGKSTLPALAQVAEREGKYLANLFNVMGKAG 420

Query: 405 GARAGSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAY 464
           G RA SAKE ELG PFVYKHLGSMATIGRYKALVDLR+SKE KGI+MAGFLSWF+WRSAY
Sbjct: 421 GGRANSAKEMELGEPFVYKHLGSMATIGRYKALVDLRESKEGKGISMAGFLSWFIWRSAY 480

Query: 465 LTRVISWRNRLYVAVNWATTFIFGRDISRI 495
           LTRV+SWRNR YVA+NW TTF+FGRDISRI
Sbjct: 481 LTRVVSWRNRFYVAINWLTTFVFGRDISRI 510

BLAST of MS014197 vs. TAIR 10
Match: AT2G29990.1 (alternative NAD(P)H dehydrogenase 2 )

HSP 1 Score: 754.6 bits (1947), Expect = 5.0e-218
Identity = 377/508 (74.21%), Postives = 423/508 (83.27%), Query Frame = 0

Query: 1   MTWFRNLRQLS------ALRSRNPRQISPSTTPFGFFPLSHFSSQSPPAARP-------- 60
           M   +NL ++S        R RN    S S T    F  +  +    PA  P        
Sbjct: 1   MFMIKNLTRISPNTSSIITRFRNSGSSSLSYTLASRFCTAQETQIQSPAKIPNDVDRSQY 60

Query: 61  PGLGPTAAGEKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTL 120
            GL PT  GEKPRVVVLGSGWAGCR+MKG+DT++YDVVCVSPRNHMVFTPLLASTCVGTL
Sbjct: 61  SGLPPTREGEKPRVVVLGSGWAGCRLMKGIDTNLYDVVCVSPRNHMVFTPLLASTCVGTL 120

Query: 121 EFRSVAEPIARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISY 180
           EFRSVAEPI+RIQPAISREPGS+FFLANC+ +D D H V CET+TDGL+T++PWKFKI+Y
Sbjct: 121 EFRSVAEPISRIQPAISREPGSFFFLANCSRLDADAHEVHCETLTDGLNTLKPWKFKIAY 180

Query: 181 DKLIIALGSQPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLL 240
           DKL+IA G++  TFGI+GV E+AIFLREV+HAQEIRRKLLLNLMLSD PGIS+EEK RLL
Sbjct: 181 DKLVIASGAEASTFGIHGVMENAIFLREVHHAQEIRRKLLLNLMLSDTPGISKEEKRRLL 240

Query: 241 HCVVVGGGPTGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANEILSSFDDRLRHYAT 300
           HCVVVGGGPTGVEFSGELSDFI +D  QRYAHVK+ I VTLIEA +ILSSFDDRLR YA 
Sbjct: 241 HCVVVGGGPTGVEFSGELSDFIMKDVRQRYAHVKDDIHVTLIEARDILSSFDDRLRRYAI 300

Query: 301 KQLTKSGVQLVRGIVKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGR 360
           KQL KSGV+ VRGIVKDV  Q +ILDDG++VPYGLLVWSTGVGPSPFV SL +PK P GR
Sbjct: 301 KQLNKSGVRFVRGIVKDVQSQKLILDDGTEVPYGLLVWSTGVGPSPFVRSLGLPKDPTGR 360

Query: 361 IGIDEWLRVPSVEDVFAIGDCSGFLESTGKPALPALAQVAERQGKYLAALLNKIGREGGA 420
           IGIDEW+RVPSV+DVFAIGDCSG+LE+TGKP LPALAQVAER+GKYLA LLN IG+  G 
Sbjct: 361 IGIDEWMRVPSVQDVFAIGDCSGYLETTGKPTLPALAQVAEREGKYLANLLNAIGKGNGG 420

Query: 421 RAGSAKEFELGAPFVYKHLGSMATIGRYKALVDLRQSKEAKGIAMAGFLSWFVWRSAYLT 480
           RA SAKE ELG PFVYKHLGSMATIGRYKALVDLR+SK+AKGI+M GF+SWF+WRSAYLT
Sbjct: 421 RANSAKEIELGVPFVYKHLGSMATIGRYKALVDLRESKDAKGISMTGFVSWFIWRSAYLT 480

Query: 481 RVISWRNRLYVAVNWATTFIFGRDISRI 495
           RVISWRNR YVA+NW TTF+FGRDISRI
Sbjct: 481 RVISWRNRFYVAINWFTTFVFGRDISRI 508

BLAST of MS014197 vs. TAIR 10
Match: AT4G28220.1 (NAD(P)H dehydrogenase B1 )

HSP 1 Score: 273.1 bits (697), Expect = 4.4e-73
Identity = 181/535 (33.83%), Postives = 263/535 (49.16%), Query Frame = 0

Query: 56  EKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI 115
           +K +VVVLG+GWAG   +K LD + YDV  VSP+N+  FTPLL S   GT+E RS+ E +
Sbjct: 48  KKKKVVVLGTGWAGISFLKDLDITSYDVQVVSPQNYFAFTPLLPSVTCGTVEARSIVESV 107

Query: 116 ARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGS 175
             I    + E     + A+C  +D     V C  V    D     +F + YD LI+A+G+
Sbjct: 108 RNITKKKNGE--IELWEADCFKIDHVNQKVHCRPVFKD-DPEASQEFSLGYDYLIVAVGA 167

Query: 176 QPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGP 235
           Q  TFG  GV E+  FL+EV  AQ IRR ++     + +PG++EE++ R LH V+VGGGP
Sbjct: 168 QVNTFGTPGVLENCHFLKEVEDAQRIRRGVIDCFEKAILPGLTEEQRRRKLHFVIVGGGP 227

Query: 236 TGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIEANE-ILSSFDDRLRHYATKQLTKSGV 295
           TGVEF+ EL DFI  D ++ Y  VK  +++TLI++ + IL++FD+R+  +A ++ T+ G+
Sbjct: 228 TGVEFAAELHDFIIEDITKIYPSVKELVKITLIQSGDHILNTFDERISSFAEQKFTRDGI 287

Query: 296 QLVRGI------VKDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGR-- 355
            +  G+       KD+T +     +   +P+GL++WSTGVG  P ++        GGR  
Sbjct: 288 DVQTGMRVMSVTDKDITVKVKSSGELVSIPHGLILWSTGVGTRPVISDFMEQVGQGGRRA 347

Query: 356 IGIDEWLRVPSVEDVFAIGDC-----------------------SGFL------------ 415
           +  +EWL+V   E+V+A+GDC                       SG L            
Sbjct: 348 VATNEWLQVTGCENVYAVGDCASIAQRKILGDIANIFKAADADNSGTLTMEELEGVVDDI 407

Query: 416 ------------------------ESTGK---------------------PALPALAQVA 475
                                   +S G                        LPA AQVA
Sbjct: 408 IVRYPQVELYLKSKHMRHINDLLADSEGNARKEVDIEAFKLALSEADSQMKTLPATAQVA 467

Query: 476 ERQGKYLAALLNKIGR-----EGGA--RAGSAKEFELGAPFVYKHLGSMATIGRYKALVD 495
            +QG YLA   N++ +     EG    R G   +F    PF YKH G  A +G  +A  +
Sbjct: 468 AQQGAYLAKCFNRMEQCKELPEGPKRFRTGGHHQFR---PFQYKHFGQFAPLGGDQAAAE 527

BLAST of MS014197 vs. TAIR 10
Match: AT4G21490.1 (NAD(P)H dehydrogenase B3 )

HSP 1 Score: 272.3 bits (695), Expect = 7.5e-73
Identity = 177/536 (33.02%), Postives = 258/536 (48.13%), Query Frame = 0

Query: 57  KPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPIA 116
           K +VV+LG+GWAG   +K L+ S Y+V  +SPRN+  FTPLL S   GT+E RSV EPI 
Sbjct: 55  KRKVVLLGTGWAGASFLKTLNNSSYEVQVISPRNYFAFTPLLPSVTCGTVEARSVVEPIR 114

Query: 117 RIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGSQ 176
            I  A  +     F  A C  +D     V C +   G+++    +F + YD L+IA G+Q
Sbjct: 115 NI--ARKQNVEMSFLEAECFKIDPGSKKVYCRS-KQGVNSKGKKEFDVDYDYLVIATGAQ 174

Query: 177 PLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGPT 236
             TF I GV+E+  FL+EV  AQ IR  ++ +   + +PG++E+E+ R+LH VVVGGGPT
Sbjct: 175 SNTFNIPGVEENCHFLKEVEDAQRIRSTVIDSFEKASLPGLNEQERKRMLHFVVVGGGPT 234

Query: 237 GVEFSGELSDFIKRDASQRYAHVKNYIRVTLIE-ANEILSSFDDRLRHYATKQLTKSGVQ 296
           GVEF+ EL DF+  D  + Y   KN +++TL+E A+ IL+ FD R+  +A ++ T+ G+ 
Sbjct: 235 GVEFASELHDFVNEDLVKLYPKAKNLVQITLLEAADHILTMFDKRITEFAEEKFTRDGID 294

Query: 297 LVRGIV------KDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGR--I 356
           +  G +      K+++ +T    + S +PYG++VWSTG+G  P +         G R  +
Sbjct: 295 VKLGSMVVKVNDKEISAKT-KAGEVSTIPYGMIVWSTGIGTRPVIKDFMKQIGQGNRRAL 354

Query: 357 GIDEWLRVPSVEDVFAIGDC-----------------------SG--------------- 416
             DEWLRV   ++++A+GDC                       SG               
Sbjct: 355 ATDEWLRVEGCDNIYALGDCATINQRKVMEDIAAIFKKADKENSGTLTMKEFHEVMSDIC 414

Query: 417 --------FLESTGK--------------------------------------PALPALA 476
                   +L+S G                                         LPA  
Sbjct: 415 DRYPQVELYLKSKGMHGITDLLKQAQAENGSNKSVELDIEELKSALCQVDSQVKLLPATG 474

Query: 477 QVAERQGKYLAALLNKI-----GREGGARAGSAKEFELGAPFVYKHLGSMATIGRYKALV 495
           QVA +QG YLA   +++       EG  R           PF Y+HLG  A +G  +   
Sbjct: 475 QVAAQQGTYLAKCFDRMEVCEKNPEGPIRIRGEGRHRF-RPFRYRHLGQFAPLGGEQTAA 534

BLAST of MS014197 vs. TAIR 10
Match: AT4G05020.1 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 266.5 bits (680), Expect = 4.1e-71
Identity = 173/537 (32.22%), Postives = 251/537 (46.74%), Query Frame = 0

Query: 56  EKPRVVVLGSGWAGCRVMKGLDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEPI 115
           +K +VV+LG+GWAG   +K L+ S Y+V  +SPRN+  FTPLL S   GT+E RSV EPI
Sbjct: 57  KKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISPRNYFAFTPLLPSVTCGTVEARSVVEPI 116

Query: 116 ARIQPAISREPGSYFFLANCTAVDTDEHLVQCETVTDGLDTVEPWKFKISYDKLIIALGS 175
             I     +   + +  A C  +D     V C +   GL +    +F + YD L+IA G+
Sbjct: 117 RNIG---RKNVDTSYLEAECFKIDPASKKVYCRS-KQGLSSNGKKEFSVDYDYLVIATGA 176

Query: 176 QPLTFGINGVKEHAIFLREVYHAQEIRRKLLLNLMLSDVPGISEEEKLRLLHCVVVGGGP 235
           Q  TF I GV+E+  FL+EV  AQ IR+ ++ +   + +P +S+EE+ R+LH VVVGGGP
Sbjct: 177 QSNTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEKASLPELSDEERKRILHFVVVGGGP 236

Query: 236 TGVEFSGELSDFIKRDASQRYAHVKNYIRVTLIE-ANEILSSFDDRLRHYATKQLTKSGV 295
           TGVEF+ EL DF+  D    Y   K  +R+TL+E A+ IL+ FD R+  +A ++ ++ G+
Sbjct: 237 TGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAADHILTMFDKRITEFAEEKFSRDGI 296

Query: 296 QLVRGIV------KDVTPQTIILDDGSKVPYGLLVWSTGVGPSPFVNSLEVPKSPGGR-- 355
            +  G +      KD++ +T    + S +PYG++VWSTG+G  P +         G R  
Sbjct: 297 DVKLGSMVTKVNEKDISAKT-KGGEVSSIPYGMIVWSTGIGTRPVIKDFMKQIGQGNRRA 356

Query: 356 IGIDEWLRVPSVEDVFAIGDC------------SGFLESTGKPA---------------- 415
           +  DEWLRV   ++++A+GDC            S       K                  
Sbjct: 357 LATDEWLRVEGTDNIYALGDCATINQRKVMEDVSAIFSKADKDKSGTLTLKEFQEAMDDI 416

Query: 416 --------------------------------------------------------LPAL 475
                                                                   LPA 
Sbjct: 417 CVRYPQVELYLKSKRMRGIADLLKEAETDDVSKNNIELKIEEFKSALSQVDSQVKFLPAT 476

Query: 476 AQVAERQGKYLAALLNKI-----GREGGARAGSAKEFELGAPFVYKHLGSMATIGRYKAL 495
           AQVA +QG YLA   +++       EG  R           PF Y+HLG  A +G  +  
Sbjct: 477 AQVAAQQGAYLAKCFDRMEECEKSPEGPIRMRGEGRHRF-RPFRYRHLGQFAPLGGEQTA 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512652.13.3e-25688.62internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [C... [more]
XP_022944050.11.7e-25588.22internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [C... [more]
XP_022986852.12.2e-25588.22internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like [C... [more]
KAG7010500.18.2e-25587.82Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial [Cucurb... [more]
KAG6570650.19.1e-25487.62Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial, partia... [more]
Match NameE-valueIdentityDescription
Q8GWA11.3e-21880.89Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Arab... [more]
O808747.1e-21774.21Internal alternative NAD(P)H-ubiquinone oxidoreductase A2, mitochondrial OS=Arab... [more]
M0ZYF33.0e-21573.24Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Sola... [more]
Q9ST634.3e-21472.84Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial OS=Sola... [more]
Q55CD91.1e-7938.32Probable NADH dehydrogenase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0270104... [more]
Match NameE-valueIdentityDescription
A0A6J1FYF88.0e-25688.22internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS... [more]
A0A6J1J8Q21.0e-25588.22internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS... [more]
A0A1S3CKL11.3e-25086.76internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like is... [more]
A0A6J1D5192.0e-243100.00internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial-like OS... [more]
A0A6J1L3V71.0e-23984.51internal alternative NAD(P)H-ubiquinone oxidoreductase A2, mitochondrial-like OS... [more]
Match NameE-valueIdentityDescription
AT1G07180.19.1e-22080.89alternative NAD(P)H dehydrogenase 1 [more]
AT2G29990.15.0e-21874.21alternative NAD(P)H dehydrogenase 2 [more]
AT4G28220.14.4e-7333.83NAD(P)H dehydrogenase B1 [more]
AT4G21490.17.5e-7333.02NAD(P)H dehydrogenase B3 [more]
AT4G05020.14.1e-7132.22NAD(P)H dehydrogenase B2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 166..184
score: 29.44
coord: 60..79
score: 28.27
coord: 346..368
score: 25.43
coord: 227..245
score: 38.65
NoneNo IPR availableGENE3D3.50.50.100coord: 51..494
e-value: 4.0E-149
score: 499.2
NoneNo IPR availablePANTHERPTHR43706:SF35NAD(P)H DEHYDROGENASE B2coord: 36..494
IPR023753FAD/NAD(P)-binding domainPFAMPF07992Pyr_redox_2coord: 59..390
e-value: 1.9E-61
score: 207.9
IPR045024Alternative NADH dehydrogenasePANTHERPTHR43706NADH DEHYDROGENASEcoord: 36..494
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 42..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014197.1MS014197.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006116 NADH oxidation
molecular_function GO:0003954 NADH dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity