MS014150 (gene) Bitter gourd (TR) v1

BLAST of MS014150 vs. NCBI nr
Match: XP_022149739.1 (transmembrane protein 56-B isoform X1 [Momordica charantia] >XP_022149740.1 transmembrane protein 56-B isoform X1 [Momordica charantia])

HSP 1 Score: 513.5 bits (1321), Expect = 1.1e-141
Identity = 259/260 (99.62%), Postives = 260/260 (100.00%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF
Sbjct: 13  ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 72

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY
Sbjct: 73  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 132

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS
Sbjct: 133 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 192

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           NFYVVNGIALFFGWLVARILLFIYFFSHMYR+FDEVKTVFPLGFYSLLAVPPVLSMMNLF
Sbjct: 193 NFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLF 252

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKTIRKARHGK
Sbjct: 253 WFWKIARGMIKTIRKARHGK 272

BLAST of MS014150 vs. NCBI nr
Match: XP_023512710.1 (transmembrane protein 56-B-like [Cucurbita pepo subsp. pepo] >XP_023512711.1 transmembrane protein 56-B-like [Cucurbita pepo subsp. pepo] >XP_023512712.1 transmembrane protein 56-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465.3 bits (1196), Expect = 3.4e-127
Identity = 226/260 (86.92%), Postives = 247/260 (95.00%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D  GP+KE+QWLAS+FSGIVFCKIVYTLTGI+S QSF+ YG+LSDFGKVEWNNRGF
Sbjct: 3   ISVTDASGPSKEVQWLASVFSGIVFCKIVYTLTGIVSLQSFRTYGKLSDFGKVEWNNRGF 62

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSDMTLGFS+GYFLSDLAMVLWY
Sbjct: 63  STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDMTLGFSVGYFLSDLAMVLWY 122

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 123 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 182

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YVVNGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLGFYSLL VPP+L++MN+F
Sbjct: 183 NSYVVNGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLALMNVF 242

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKT+RKARH K
Sbjct: 243 WFWKIARGMIKTLRKARHNK 262

BLAST of MS014150 vs. NCBI nr
Match: XP_022986922.1 (transmembrane protein 56-B-like [Cucurbita maxima] >XP_022986923.1 transmembrane protein 56-B-like [Cucurbita maxima] >XP_022986924.1 transmembrane protein 56-B-like [Cucurbita maxima])

HSP 1 Score: 463.8 bits (1192), Expect = 1.0e-126
Identity = 226/260 (86.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D  GP+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NRGF
Sbjct: 3   ISVTDASGPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNRGF 62

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSD TLGFS+GYFLSDLAMVLWY
Sbjct: 63  STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDTTLGFSVGYFLSDLAMVLWY 122

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 123 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 182

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YV+NGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLGFYSLL VPP+L+MMN+F
Sbjct: 183 NSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMNVF 242

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKT+RKARH K
Sbjct: 243 WFWKIARGMIKTLRKARHNK 262

BLAST of MS014150 vs. NCBI nr
Match: KAG6570610.1 (TLC domain-containing protein 4-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 463.0 bits (1190), Expect = 1.7e-126
Identity = 225/260 (86.54%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D  GP KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEWNNRGF
Sbjct: 48  ISVNDVSGPGKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWNNRGF 107

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRR+SSLSDMTLGFS+GYFLSDLAMVLWY
Sbjct: 108 STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRSSSLSDMTLGFSVGYFLSDLAMVLWY 167

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 168 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 227

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YV+NGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLG YSLL VPP+L+MMN+F
Sbjct: 228 NSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGLYSLLTVPPMLAMMNVF 287

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKI+RGMIKT+RKARH K
Sbjct: 288 WFWKISRGMIKTLRKARHNK 307

BLAST of MS014150 vs. NCBI nr
Match: XP_022944198.1 (transmembrane protein 56-B [Cucurbita moschata] >XP_022944199.1 transmembrane protein 56-B [Cucurbita moschata] >XP_022944200.1 transmembrane protein 56-B [Cucurbita moschata])

HSP 1 Score: 462.6 bits (1189), Expect = 2.2e-126
Identity = 226/260 (86.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D   P+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NRGF
Sbjct: 3   ISVTDVSVPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNRGF 62

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSDMTLGFS+GYFLSDLAMVLWY
Sbjct: 63  STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDMTLGFSVGYFLSDLAMVLWY 122

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 123 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 182

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YV+NGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLGFYSLL VPP+L+MMN+F
Sbjct: 183 NSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMNVF 242

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKT+RKARH K
Sbjct: 243 WFWKIARGMIKTLRKARHNK 262

BLAST of MS014150 vs. ExPASy Swiss-Prot
Match: Q6PGS5 (TLC domain-containing protein 4-B OS=Xenopus laevis OX=8355 GN=tlcd4-b PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.3e-16
Identity = 67/241 (27.80%), Postives = 118/241 (48.96%), Query Frame = 0

Query: 18  SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 77
           S+ S  VF  + +  +  +S +    + +LS   K+EWN+R  S+FHALV     LY+L+
Sbjct: 12  SVTSLAVFQFLFHVGSSWLSTRLTGGFHKLSARQKIEWNSRTVSSFHALVVGCFCLYILV 71

Query: 78  LSSTFH--PGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHAL 137
                +  P   D  +++       + +  + GY +SDL ++++Y+  +G   +V HH  
Sbjct: 72  YDDAVNADPVWGDPFMVK-------LNVAVTSGYLISDLLLIIYYWKEIGDKYFVTHHLA 131

Query: 138 SLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAG-NKNSNFYVVNGIALFFGW 197
           +L++    L  G    +    L  E +TPFVN RW+ +  G +K S   +VNG+ +   +
Sbjct: 132 ALYACYYVLGEGMLPYFGNFRLIAEFSTPFVNQRWFFEVLGYSKYSLPNMVNGVLMTISF 191

Query: 198 LVARILLFIYFFSHMYRHF-DEVKTVFPLGFYSLLAVPPV-LSMMNLFWFWKIARGMIKT 254
            + RI +   ++  ++  F  E      LG      +  V L +MN+ W  KIA+G  K 
Sbjct: 192 FIVRIAVIPIYYGRVFSTFGTEAFHRLGLGAQCAWIISSVSLDIMNVMWMIKIAKGCYKV 245

BLAST of MS014150 vs. ExPASy Swiss-Prot
Match: Q96MV1 (TLC domain-containing protein 4 OS=Homo sapiens OX=9606 GN=TLCD4 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.8e-15
Identity = 72/255 (28.24%), Postives = 119/255 (46.67%), Query Frame = 0

Query: 10  NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 69
           N +L    +  S   F  + Y ++   S +    +  LS   K+EWN+R  ST H+LV  
Sbjct: 4   NTKLLISVTCISFFTFQLLFYFVSYWFSAKVSPGFNSLSFKKKIEWNSRVVSTCHSLVVG 63

Query: 70  SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 129
              LY+ L         +D L      SL+++ +  + GY +SDL++++ Y+  +G   +
Sbjct: 64  IFGLYIFLFDEA---TKADPLW--GGPSLANVNIAIASGYLISDLSIIILYWKVIGDKFF 123

Query: 130 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAG-NKNSNFYVVNGI 189
           ++HH  SL++    L +G         L  E ++PFVN RW+ +     K S   V+NGI
Sbjct: 124 IMHHCASLYAYYLVLKNGVLAYIGNFRLLAELSSPFVNQRWFFEALKYPKFSKAIVINGI 183

Query: 190 ALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKI 249
            +   + + RI   +  +  MY  +   +    LG     S +    VL +MN+ W  KI
Sbjct: 184 LMTVVFFIVRIASMLPHYGFMYSVYG-TEPYIRLGVLIQLSWVISCVVLDVMNVMWMIKI 243

Query: 250 ARGMIKTIRKARHGK 261
           ++G IK I   R  K
Sbjct: 244 SKGCIKVISHIRQEK 252

BLAST of MS014150 vs. ExPASy Swiss-Prot
Match: Q6P4N1 (TLC domain-containing protein 4 OS=Xenopus tropicalis OX=8364 GN=tlcd4 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.3e-15
Identity = 71/239 (29.71%), Postives = 113/239 (47.28%), Query Frame = 0

Query: 32  LTGIISFQSFKAYG---RLSDFGK-VEWNNRGFSTFHALVAASSSLYLLLLSSTFH--PG 91
           L  IIS ++F  Y    R   FGK  EW++R  ST HALV  S  LY+L      +  P 
Sbjct: 22  LFSIISPRTFTKYSSTYRQLSFGKQCEWDSRCVSTTHALVVGSGCLYILAYDEAVNADPI 81

Query: 92  SSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLL 151
             D   ++       M +  + GY + DL ++  ++  +     V HH    +S    L 
Sbjct: 82  WGDPFWVK-------MNVAITCGYLVHDLLLLARFWKVMRDPYMVCHHLAVFYSYGYVLN 141

Query: 152 SGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYV-VNGIALFFGWLVARILLFIY 211
            G    +    L +E +TPFVN RW+ D  G   S++ V +NG+A+   + + RI +   
Sbjct: 142 RGVLPYFANFRLISELSTPFVNQRWFFDVIGKPRSSWPVLLNGLAMALVFFIVRIAVIPS 201

Query: 212 FFSHMYRHFDEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 261
           ++S ++  F   +    LG     + +    VL ++N+FW +KIARG  K ++    GK
Sbjct: 202 YYSQVFATFG-TEGYIRLGIGPQVAWIVSCVVLDILNVFWMYKIARGFYKVVKAKPDGK 252

BLAST of MS014150 vs. ExPASy Swiss-Prot
Match: Q6GLX2 (TLC domain-containing protein 4-A OS=Xenopus laevis OX=8355 GN=tlcd4-a PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-14
Identity = 65/230 (28.26%), Postives = 109/230 (47.39%), Query Frame = 0

Query: 38  FQSFKAYGRLSDFGK-VEWNNRGFSTFHALVAASSSLYLLLLSSTFH--PGSSDELLIRR 97
           F  + +  R   FGK  EW++R  ST HAL+  S+ LY+L      +  P   D   ++ 
Sbjct: 30  FTKYSSTYRQLSFGKQCEWDSRFVSTNHALIVGSACLYILAYDDAVNADPIWGDPFWVK- 89

Query: 98  TSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIYIL 157
                 M +  + GY + DL ++  ++  +     V HH    +S    L  G    +  
Sbjct: 90  ------MNVAITCGYLVQDLLLLARFWKVMRDPYMVCHHLAVFYSYGYVLNRGVLPYFAN 149

Query: 158 MVLFTESTTPFVNLRWYLDNAGNKNSNFYV-VNGIALFFGWLVARILLFIYFFSHMYRHF 217
             L +E +TPFVN RW+ D  G   S++ V +NG+A+   + + RI +   ++S ++  F
Sbjct: 150 FRLISELSTPFVNQRWFFDVIGKPRSSWPVLLNGLAMALVFFIVRIAVIPSYYSQVFATF 209

Query: 218 DEVKTVFPLGF---YSLLAVPPVLSMMNLFWFWKIARGMIKTIRKARHGK 261
              +    LG     + +    VL ++N+FW +KIARG  K ++    GK
Sbjct: 210 G-TEGYIRLGIGPQVAWIVSCVVLDILNVFWMYKIARGFYKVVKAKPDGK 251

BLAST of MS014150 vs. ExPASy Swiss-Prot
Match: Q550S9 (TLC domain-containing protein 4 B OS=Dictyostelium discoideum OX=44689 GN=tlcd4b PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.1e-14
Identity = 60/207 (28.99%), Postives = 106/207 (51.21%), Query Frame = 0

Query: 52  KVEWNNRGFSTFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFL 111
           ++EW NR  ST +A+V ++ S+Y L  +            +R TS +S     F   YF+
Sbjct: 46  RIEWPNRIISTVNAIVTSALSIYCLYYNEWIVNS------LRSTSEMSYFIFKFITYYFI 105

Query: 112 SDLAMVLWYFPALGGLEYVLHHALSLFSIVQSLLSGQGQIY--ILMVLFTESTTPFVNLR 171
            D  +  +Y   L     +LHH ++L S   + L G+G  +  +L   FTE TTP +NLR
Sbjct: 106 YDFIISSYYSKYLFTWGNLLHHTIALLSF--TFLGGKGLAHHLLLSYTFTEITTPLINLR 165

Query: 172 WYLDNAGNKNSNFYVVNGIALFFGWLVARIL-----LFIYFFSHMYRHFDEVKTVFPLGF 231
           ++L +   KN   YV+NG+ +F G+++ R+      +F   F+  +   +      PL  
Sbjct: 166 FFLLDLNLKNHPLYVINGLLIFVGFVLFRVFYTSATMFDVIFNQPHYSIE----TDPLIP 225

Query: 232 YSLLAVPPVLSMMNLFWFWKIARGMIK 252
           + +  V P ++++NL+W + I++ + K
Sbjct: 226 FFINFVYPAITLLNLYWTFYISKSIFK 240

BLAST of MS014150 vs. ExPASy TrEMBL
Match: A0A6J1D7X6 (transmembrane protein 56-B isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018098 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 5.3e-142
Identity = 259/260 (99.62%), Postives = 260/260 (100.00%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF
Sbjct: 13  ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 72

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY
Sbjct: 73  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 132

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS
Sbjct: 133 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 192

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           NFYVVNGIALFFGWLVARILLFIYFFSHMYR+FDEVKTVFPLGFYSLLAVPPVLSMMNLF
Sbjct: 193 NFYVVNGIALFFGWLVARILLFIYFFSHMYRNFDEVKTVFPLGFYSLLAVPPVLSMMNLF 252

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKTIRKARHGK
Sbjct: 253 WFWKIARGMIKTIRKARHGK 272

BLAST of MS014150 vs. ExPASy TrEMBL
Match: A0A6J1JHF4 (transmembrane protein 56-B-like OS=Cucurbita maxima OX=3661 GN=LOC111484516 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 4.8e-127
Identity = 226/260 (86.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D  GP+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NRGF
Sbjct: 3   ISVTDASGPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNRGF 62

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSD TLGFS+GYFLSDLAMVLWY
Sbjct: 63  STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDTTLGFSVGYFLSDLAMVLWY 122

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 123 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 182

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YV+NGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLGFYSLL VPP+L+MMN+F
Sbjct: 183 NSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMNVF 242

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKT+RKARH K
Sbjct: 243 WFWKIARGMIKTLRKARHNK 262

BLAST of MS014150 vs. ExPASy TrEMBL
Match: A0A6J1FYI8 (transmembrane protein 56-B OS=Cucurbita moschata OX=3662 GN=LOC111448719 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.1e-126
Identity = 226/260 (86.92%), Postives = 245/260 (94.23%), Query Frame = 0

Query: 1   ISVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGF 60
           ISV+D   P+KELQWLAS+FSGIVFCKIVYTLTGI+S QSFK YG+LSDFGKVEW NRGF
Sbjct: 3   ISVTDVSVPSKELQWLASVFSGIVFCKIVYTLTGIVSLQSFKTYGKLSDFGKVEWKNRGF 62

Query: 61  STFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWY 120
           STFHALVAASSSLYL+LLSSTF PGSS+ELLIRRTSSLSDMTLGFS+GYFLSDLAMVLWY
Sbjct: 63  STFHALVAASSSLYLVLLSSTFDPGSSNELLIRRTSSLSDMTLGFSVGYFLSDLAMVLWY 122

Query: 121 FPALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNS 180
           FPALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNS
Sbjct: 123 FPALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTECTTPFVNLRWYLDNAGQKNS 182

Query: 181 NFYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLF 240
           N YV+NGIALF GWLVARILLFIYFF HM++HFD+VKTV+PLGFYSLL VPP+L+MMN+F
Sbjct: 183 NSYVINGIALFLGWLVARILLFIYFFGHMFKHFDQVKTVYPLGFYSLLTVPPMLAMMNVF 242

Query: 241 WFWKIARGMIKTIRKARHGK 261
           WFWKIARGMIKT+RKARH K
Sbjct: 243 WFWKIARGMIKTLRKARHNK 262

BLAST of MS014150 vs. ExPASy TrEMBL
Match: A0A6J1L4D0 (transmembrane protein 56-B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499774 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.1e-126
Identity = 224/259 (86.49%), Postives = 248/259 (95.75%), Query Frame = 0

Query: 2   SVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFS 61
           SV+D   P+K LQWLAS+FSGIVFCKIVYTLTGI+S QSFKAYG+LSDFGKVEWNNRGFS
Sbjct: 4   SVTDASDPSKGLQWLASVFSGIVFCKIVYTLTGILSLQSFKAYGQLSDFGKVEWNNRGFS 63

Query: 62  TFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYF 121
           TFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIGYFLSDLAMVLW+F
Sbjct: 64  TFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWFF 123

Query: 122 PALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSN 181
           PALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNSN
Sbjct: 124 PALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNLRWYLDNAGQKNSN 183

Query: 182 FYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFW 241
            YV+NGIALFFGWLVARI+LFIYFF HM++HFDEVK+V+PLGFY+LLAVPP+L++MN+FW
Sbjct: 184 LYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYPLGFYTLLAVPPMLAVMNVFW 243

Query: 242 FWKIARGMIKTIRKARHGK 261
           FWKIARGMIKT++KARH K
Sbjct: 244 FWKIARGMIKTLKKARHSK 262

BLAST of MS014150 vs. ExPASy TrEMBL
Match: A0A6J1L744 (transmembrane protein 56-B-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499774 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.1e-126
Identity = 224/259 (86.49%), Postives = 248/259 (95.75%), Query Frame = 0

Query: 2   SVSDTPGPNKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFS 61
           SV+D   P+K LQWLAS+FSGIVFCKIVYTLTGI+S QSFKAYG+LSDFGKVEWNNRGFS
Sbjct: 26  SVTDASDPSKGLQWLASVFSGIVFCKIVYTLTGILSLQSFKAYGQLSDFGKVEWNNRGFS 85

Query: 62  TFHALVAASSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYF 121
           TFHALVAASSSLYL+LLSSTF+PGSS+ELLIRRTSSLSDMTLGFSIGYFLSDLAMVLW+F
Sbjct: 86  TFHALVAASSSLYLVLLSSTFNPGSSNELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWFF 145

Query: 122 PALGGLEYVLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSN 181
           PALGGLEYVLHH LSLFSI+QSLLSGQGQ+YILMVLFTE TTPFVNLRWYLDNAG KNSN
Sbjct: 146 PALGGLEYVLHHGLSLFSIIQSLLSGQGQVYILMVLFTEITTPFVNLRWYLDNAGQKNSN 205

Query: 182 FYVVNGIALFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFW 241
            YV+NGIALFFGWLVARI+LFIYFF HM++HFDEVK+V+PLGFY+LLAVPP+L++MN+FW
Sbjct: 206 LYVINGIALFFGWLVARIVLFIYFFGHMFKHFDEVKSVYPLGFYTLLAVPPMLAVMNVFW 265

Query: 242 FWKIARGMIKTIRKARHGK 261
           FWKIARGMIKT++KARH K
Sbjct: 266 FWKIARGMIKTLKKARHSK 284

BLAST of MS014150 vs. TAIR 10
Match: AT4G10360.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 146/248 (58.87%), Postives = 187/248 (75.40%), Query Frame = 0

Query: 10  NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 69
           +++L  LASI SG + CKIVY LT  IS   F  YG+L    ++EWNNRGFSTFHA+  +
Sbjct: 12  SRQLLLLASICSGALMCKIVYDLTRFISPLLFSVYGKLDSKVRMEWNNRGFSTFHAVFTS 71

Query: 70  SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 129
            +S+Y L++S  F      + +I  T+ LS+  +G S+GYFL+DLAM+ WYFP LGG+EY
Sbjct: 72  VASIYFLVISDQFDENVHGDSVINSTTRLSESVMGISLGYFLADLAMIFWYFPTLGGIEY 131

Query: 130 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIA 189
           V HH LS+F+I+ S+ SGQ Q YI +VL +E+TTPFVNLRWYLDN+G K S  Y +NGIA
Sbjct: 132 VFHHFLSMFAIILSVTSGQSQFYIFLVLLSEATTPFVNLRWYLDNSGQKGSKAYTLNGIA 191

Query: 190 LFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGM 249
           LF GWLVAR+LLFI+FF HMY HF +VK VFPLGFYSLL +PP L++MNL WFWKI +G+
Sbjct: 192 LFLGWLVARVLLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLLWFWKITKGL 251

Query: 250 IKTIRKAR 258
           IKT+ KA+
Sbjct: 252 IKTLSKAK 259

BLAST of MS014150 vs. TAIR 10
Match: AT4G10360.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 146/248 (58.87%), Postives = 187/248 (75.40%), Query Frame = 0

Query: 10  NKELQWLASIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAA 69
           +++L  LASI SG + CKIVY LT  IS   F  YG+L    ++EWNNRGFSTFHA+  +
Sbjct: 12  SRQLLLLASICSGALMCKIVYDLTRFISPLLFSVYGKLDSKVRMEWNNRGFSTFHAVFTS 71

Query: 70  SSSLYLLLLSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEY 129
            +S+Y L++S  F      + +I  T+ LS+  +G S+GYFL+DLAM+ WYFP LGG+EY
Sbjct: 72  VASIYFLVISDQFDENVHGDSVINSTTRLSESVMGISLGYFLADLAMIFWYFPTLGGIEY 131

Query: 130 VLHHALSLFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIA 189
           V HH LS+F+I+ S+ SGQ Q YI +VL +E+TTPFVNLRWYLDN+G K S  Y +NGIA
Sbjct: 132 VFHHFLSMFAIILSVTSGQSQFYIFLVLLSEATTPFVNLRWYLDNSGQKGSKAYTLNGIA 191

Query: 190 LFFGWLVARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGM 249
           LF GWLVAR+LLFI+FF HMY HF +VK VFPLGFYSLL +PP L++MNL WFWKI +G+
Sbjct: 192 LFLGWLVARVLLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLLWFWKITKGL 251

Query: 250 IKTIRKAR 258
           IKT+ KA+
Sbjct: 252 IKTLSKAK 259

BLAST of MS014150 vs. TAIR 10
Match: AT1G31300.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.4e-62
Identity = 118/238 (49.58%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 18  SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 77
           S+ +GI  CK+VY L   IS    K Y  L+   ++EWNNRG ST HA+  ++ SLY + 
Sbjct: 37  SVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIEWNNRGISTVHAIFISAMSLYFVF 96

Query: 78  LSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSL 137
            S  F     ++L++ R+S LS + LG SIGYFL+DL M+ W +P+LGG+EY++HH+LS 
Sbjct: 97  WSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADLGMIFWKYPSLGGIEYIVHHSLSG 156

Query: 138 FSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLVA 197
            ++  SL SG+GQ+Y  MVL +E TTP +NLRWYLD AG K S  YVVNG+ +F  WLVA
Sbjct: 157 VAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDTAGMKKSLAYVVNGVFIFLAWLVA 216

Query: 198 RILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 256
           RILLFIY F H+Y H+++V  +   G+  +  VP  L +MNL WF KI RG+ KT+ K
Sbjct: 217 RILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAALGVMNLIWFGKIVRGVKKTLAK 274

BLAST of MS014150 vs. TAIR 10
Match: AT1G31300.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.4e-62
Identity = 118/238 (49.58%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 18  SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 77
           S+ +GI  CK+VY L   IS    K Y  L+   ++EWNNRG ST HA+  ++ SLY + 
Sbjct: 37  SVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIEWNNRGISTVHAIFISAMSLYFVF 96

Query: 78  LSSTFHPGSSDELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALSL 137
            S  F     ++L++ R+S LS + LG SIGYFL+DL M+ W +P+LGG+EY++HH+LS 
Sbjct: 97  WSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADLGMIFWKYPSLGGIEYIVHHSLSG 156

Query: 138 FSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLVA 197
            ++  SL SG+GQ+Y  MVL +E TTP +NLRWYLD AG K S  YVVNG+ +F  WLVA
Sbjct: 157 VAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDTAGMKKSLAYVVNGVFIFLAWLVA 216

Query: 198 RILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 256
           RILLFIY F H+Y H+++V  +   G+  +  VP  L +MNL WF KI RG+ KT+ K
Sbjct: 217 RILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAALGVMNLIWFGKIVRGVKKTLAK 274

BLAST of MS014150 vs. TAIR 10
Match: AT4G19645.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 118/239 (49.37%), Postives = 155/239 (64.85%), Query Frame = 0

Query: 18  SIFSGIVFCKIVYTLTGIISFQSFKAYGRLSDFGKVEWNNRGFSTFHALVAASSSLYLLL 77
           S+  GI  CK+VY LT + S    K+Y  L+   ++EWNNRG ST HA+  +  +LY   
Sbjct: 28  SVLVGICLCKLVYDLTELFSSIHVKSYSALTKIKRIEWNNRGISTVHAIFISFMALYFAF 87

Query: 78  LSSTFHPGSS-DELLIRRTSSLSDMTLGFSIGYFLSDLAMVLWYFPALGGLEYVLHHALS 137
            S  F    S + L + R S LS   LG S+GYFL+DL M+ W +P+LGG EY+LHH LS
Sbjct: 88  FSDLFSDQRSLETLTVFRNSPLSTFGLGVSVGYFLADLGMIFWLYPSLGGSEYILHHCLS 147

Query: 138 LFSIVQSLLSGQGQIYILMVLFTESTTPFVNLRWYLDNAGNKNSNFYVVNGIALFFGWLV 197
             ++  SL SG+ Q+Y  MVL +E TTP +NLRWYLD AG K S  Y+VNG+A+FF WL 
Sbjct: 148 GTAVAYSLFSGEAQLYTYMVLISEVTTPEINLRWYLDIAGLKRSKAYLVNGVAIFFAWLT 207

Query: 198 ARILLFIYFFSHMYRHFDEVKTVFPLGFYSLLAVPPVLSMMNLFWFWKIARGMIKTIRK 256
           ARILLFIY F H+Y H+D+V  +   G+  +  VP  LS+MNL WF KI +G+ KT+ K
Sbjct: 208 ARILLFIYMFYHVYTHYDQVIEMHTFGYLLVFVVPISLSVMNLIWFGKIVKGLKKTLEK 266