MS014079 (gene) Bitter gourd (TR) v1

Overview


Name	MS014079
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	heat stress transcription factor A-4c-like
Location	scaffold5: 433268 .. 433995 (-)
RNA-Seq Expression	MS014079
Synteny	MS014079

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACCAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCATAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGTGAGTTCGCATCAGATCATCAAATTTCACAACCCCTTGTCGTCGTTTTCCCCTGTTTTTTTCCTAACATTAATCAATTCCTCTGTTTTTAGTTAAAATCCTGAGAGCAAAATTGTGGTATCTCTGTTTAGGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCATAGCCATAGCCAGAGCTCCAATGGATCTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGACCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGGCGGCTCAATTGCAGAAG

mRNA sequence

ATGGACGGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACCAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCATAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCATAGCCATAGCCAGAGCTCCAATGGATCTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGACCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGGCGGCTCAATTGCAGAAG

Coding sequence (CDS)

ATGGACGGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACCAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCATAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCATAGCCATAGCCAGAGCTCCAATGGATCTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGACCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGGCGGCTCAATTGCAGAAG

Protein sequence

MDGSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQLITMLAAQLQK

Homology

[Close]

BLAST of MS014079 vs. NCBI nr
Match: XP_022149724.1 (heat stress transcription factor A-4c-like [Momordica charantia])

HSP 1 Score: 380.2 bits (975), Expect = 1.1e-101
Identity = 194/199 (97.49%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   MDGSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MD SEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN
Sbjct: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG
Sbjct: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120

Query: 121 HSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
            SHSQSSNG PFSEPERQELENKIK LNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW
Sbjct: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180

Query: 181 RMGNEQKQLITMLAAQLQK 200
           RMGNEQKQLITML AQLQK
Sbjct: 181 RMGNEQKQLITMLTAQLQK 199

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BLAST of MS014079 vs. NCBI nr
Match: KAG7034870.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.1 bits (775), Expect = 1.7e-78
Identity = 152/200 (76.00%), Postives = 179/200 (89.50%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+Q
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSQ 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
            H      +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 SH-----GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 195

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BLAST of MS014079 vs. NCBI nr
Match: KAG6604741.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 151/200 (75.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 193

[Close]

BLAST of MS014079 vs. NCBI nr
Match: XP_022970770.1 (heat stress transcription factor A-4c-like [Cucurbita maxima])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 151/200 (75.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 193

[Close]

BLAST of MS014079 vs. NCBI nr
Match: XP_023534238.1 (heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 151/200 (75.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 193

[Close]

BLAST of MS014079 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.3e-48
Identity = 99/194 (51.03%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           VSS + PPFLTKTYEMVDD S++++VSWSQS  SF+VWNPPEF+++LLP +FKHNNFSSF
Sbjct: 8   VSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSF 67

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQ 125
           +RQLNTYGFRK D EQWEFAN+ FVRG+ HL++ IHRRKP++SHS     +Q        
Sbjct: 68  IRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQL------- 127

Query: 126 SSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
               +P ++ ER  + N+I+ L +E   L  +L K + E+E  E Q++ L  +L  M   
Sbjct: 128 ----NPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKR 187

Query: 186 QKQLITMLAAQLQK 200
           QK +++ ++  L+K
Sbjct: 188 QKTMVSFVSQVLEK 190

[Close]

BLAST of MS014079 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.6e-47
Identity = 103/198 (52.02%), Postives = 132/198 (66.67%), Query Frame = 0


Query: 3   GSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNF 62
           G     G PPPFL KTYEMV+D +TN VVSW   G SFVVWNP +F+++LLP YFKHNNF
Sbjct: 10  GGGGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNF 69

Query: 63  SSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHS-QNQSHSQNQGH 122
           SSF+RQLNTYGFRKID E+WEFANE F+RG THLL+ IHRRKP++SHS QNQ +      
Sbjct: 70  SSFIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQIN------ 129

Query: 123 SHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWR 182
                    P +E ER+ELE +I  L  E + L + LQ+  +++  I  Q+Q++  +L  
Sbjct: 130 --------GPLAESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVA 189

Query: 183 MGNEQKQLITMLAAQLQK 200
           M   QK ++  L   LQ+
Sbjct: 190 MEQRQKNIVASLCEMLQR 193

[Close]

BLAST of MS014079 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.6e-47
Identity = 100/183 (54.64%), Postives = 135/183 (73.77%), Query Frame = 0


Query: 9   GAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQ 68
           G+ PPFL+KTYEMVDDPST+AVV W+ +G SFVV N PEF ++LLP YFKHNNFSSFVRQ
Sbjct: 8   GSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNFSSFVRQ 67

Query: 69  LNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQSSN 128
           LNTYGFRK+D EQWEFANE F++G+ H L+ IHRRKPI+SHS   SHSQ  G        
Sbjct: 68  LNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQGAG-------- 127

Query: 129 GSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQ 188
             P ++ ER++ E +I+ L  +N  L S+LQ +  +K  +E+++Q+L  +L+ + ++Q+ 
Sbjct: 128 --PLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRS 177

Query: 189 LIT 192
           LI+
Sbjct: 188 LIS 177

[Close]

BLAST of MS014079 vs. ExPASy Swiss-Prot
Match: Q9LQM7 (Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 6.9e-46
Identity = 98/193 (50.78%), Postives = 143/193 (74.09%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           +SS APPPFL+KTY+MVDD +T+++VSWS +  SF+VW PPEFA++LLP  FKHNNFSSF
Sbjct: 30  LSSNAPPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSF 89

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQ 125
           VRQLNTYGFRK+D ++WEFANEGF+RG+ HLL++I RRKP  +H Q Q H ++Q HS+ Q
Sbjct: 90  VRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKP--AHGQGQGHQRSQ-HSNGQ 149

Query: 126 SSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
           +S+ S   E  +  LE +++ L ++   L  +L +  ++++  + Q+Q++  +L  M N 
Sbjct: 150 NSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENR 209

Query: 186 QKQLITMLAAQLQ 199
           Q+QL++ LA  +Q
Sbjct: 210 QQQLMSFLAKAVQ 219

[Close]

BLAST of MS014079 vs. ExPASy Swiss-Prot
Match: P41153 (Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.4e-45
Identity = 96/192 (50.00%), Postives = 135/192 (70.31%), Query Frame = 0


Query: 7   SSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFV 66
           S+ APPPFL KTY+MVDDPST+ +VSWS +  SFVVW+PPEFAK+LLP YFKHNNFSSFV
Sbjct: 35  SANAPPPFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFV 94

Query: 67  RQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQ----SHSQNQGHS 126
           RQLNTYGFRK+D ++WEFANEGF+RG+ HLL++I RRKP + H+Q Q     H+Q Q   
Sbjct: 95  RQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQQQPHGHAQQQMQP 154

Query: 127 HSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRM 186
              S++     E  +  LE +++ L ++   L  +L +  ++++  + Q+Q +  +L  M
Sbjct: 155 PGHSASVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQGMVQRLQGM 214

Query: 187 GNEQKQLITMLA 195
              Q+Q+++ LA
Sbjct: 215 ELRQQQMMSFLA 226

[Close]

BLAST of MS014079 vs. ExPASy TrEMBL
Match: A0A6J1D7W3 (heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC111018080 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.4e-102
Identity = 194/199 (97.49%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   MDGSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MD SEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN
Sbjct: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG
Sbjct: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120

Query: 121 HSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
            SHSQSSNG PFSEPERQELENKIK LNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW
Sbjct: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180

Query: 181 RMGNEQKQLITMLAAQLQK 200
           RMGNEQKQLITML AQLQK
Sbjct: 181 RMGNEQKQLITMLTAQLQK 199

[Close]

BLAST of MS014079 vs. ExPASy TrEMBL
Match: A0A6J1I3T7 (heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111469660 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.4e-78
Identity = 151/200 (75.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 193

[Close]

BLAST of MS014079 vs. ExPASy TrEMBL
Match: A0A0A0KBI1 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 7.8e-77
Identity = 152/200 (76.00%), Postives = 170/200 (85.00%), Query Frame = 0


Query: 1   MDGSEVSS-GAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           MDGSE SS GAPPPFLTKTYEMVDDP TN++VSW+QSGFSFVVWNPPEFA+ELLPIYFKH
Sbjct: 1   MDGSEGSSTGAPPPFLTKTYEMVDDPMTNSIVSWNQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRKIDREQWEFANEGF+RG+THLL++IHRRKPIYSHSQ+       
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPIYSHSQS------- 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
               SQ + G+P SE ER ELE KIKTL QE T LQSQLQKHE EKE+I  QIQ +C +L
Sbjct: 121 ----SQGNGGAPLSEQERHELEQKIKTLYQEKTNLQSQLQKHENEKEQIGHQIQRICEKL 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI +L A+L+K
Sbjct: 181 WRMGNQQKQLIGILGAELEK 189

[Close]

BLAST of MS014079 vs. ExPASy TrEMBL
Match: A0A6J1G8J3 (heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC111451807 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 149/200 (74.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 1   MDGSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+GS+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS S   
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSH-- 120

Query: 121 GHSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
                   +G+P SE ERQELE KIKTL+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 -------GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLAAQLQK 200
           WRMGN+QKQLI ++AA+LQK
Sbjct: 181 WRMGNQQKQLIAIMAAELQK 191

[Close]

BLAST of MS014079 vs. ExPASy TrEMBL
Match: A0A5D3BGW6 (Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G00240 PE=3 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 9.6e-75
Identity = 145/193 (75.13%), Postives = 167/193 (86.53%), Query Frame = 0


Query: 7   SSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFV 66
           SSGAPPPFLTKTYEMVDDP +N++VSWSQSGFSFVVWNPPEFA+ELLPIYFKHNNFSSFV
Sbjct: 8   SSGAPPPFLTKTYEMVDDPMSNSIVSWSQSGFSFVVWNPPEFAQELLPIYFKHNNFSSFV 67

Query: 67  RQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQS 126
           RQLNTYGFRKIDREQWEFANEGF+RG+THLL++IHRRKP+YSHSQ+           SQ 
Sbjct: 68  RQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPMYSHSQS-----------SQG 127

Query: 127 SNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQ 186
           + G+P SE ERQELE+KIKTL QE T L+SQLQKHE EKE+I  QIQ +C +LWRMG++Q
Sbjct: 128 NGGAPLSEQERQELEHKIKTLYQEKTNLESQLQKHENEKEQIGHQIQRICEKLWRMGHQQ 187

Query: 187 KQLITMLAAQLQK 200
           KQLI +L A+L+K
Sbjct: 188 KQLIGILGAELEK 189

[Close]

BLAST of MS014079 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 192.6 bits (488), Expect = 3.1e-49
Identity = 99/194 (51.03%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           VSS + PPFLTKTYEMVDD S++++VSWSQS  SF+VWNPPEF+++LLP +FKHNNFSSF
Sbjct: 8   VSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSF 67

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQ 125
           +RQLNTYGFRK D EQWEFAN+ FVRG+ HL++ IHRRKP++SHS     +Q        
Sbjct: 68  IRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQL------- 127

Query: 126 SSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
               +P ++ ER  + N+I+ L +E   L  +L K + E+E  E Q++ L  +L  M   
Sbjct: 128 ----NPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKR 187

Query: 186 QKQLITMLAAQLQK 200
           QK +++ ++  L+K
Sbjct: 188 QKTMVSFVSQVLEK 190

[Close]

BLAST of MS014079 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 185.3 bits (469), Expect = 4.9e-47
Identity = 98/193 (50.78%), Postives = 143/193 (74.09%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           +SS APPPFL+KTY+MVDD +T+++VSWS +  SF+VW PPEFA++LLP  FKHNNFSSF
Sbjct: 30  LSSNAPPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSF 89

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQ 125
           VRQLNTYGFRK+D ++WEFANEGF+RG+ HLL++I RRKP  +H Q Q H ++Q HS+ Q
Sbjct: 90  VRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKP--AHGQGQGHQRSQ-HSNGQ 149

Query: 126 SSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
           +S+ S   E  +  LE +++ L ++   L  +L +  ++++  + Q+Q++  +L  M N 
Sbjct: 150 NSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENR 209

Query: 186 QKQLITMLAAQLQ 199
           Q+QL++ LA  +Q
Sbjct: 210 QQQLMSFLAKAVQ 219

[Close]

BLAST of MS014079 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 181.4 bits (459), Expect = 7.1e-46
Identity = 95/199 (47.74%), Postives = 137/199 (68.84%), Query Frame = 0


Query: 1   MDGSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MD +   S + PPFLTKTYEMVDD S+++VV+WS++  SF+V NP EF+++LLP +FKH 
Sbjct: 1   MDENNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKHK 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSF+RQLNTYGFRK+D E+WEF N+ FVRGR +L++ IHRRKP++SHS     +QN  
Sbjct: 61  NFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN-- 120

Query: 121 HSHSQSSNGSPFSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
                     P +E ER+ +E++I+ L  E   L ++LQ  E+E++E E Q+ +L ++L 
Sbjct: 121 ----------PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQ 180

Query: 181 RMGNEQKQLITMLAAQLQK 200
            M   QK ++  ++  L K
Sbjct: 181 HMEQHQKSIVAYVSQVLGK 187

[Close]

BLAST of MS014079 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 174.5 bits (441), Expect = 8.6e-44
Identity = 92/194 (47.42%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 7   SSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFV 66
           ++  PPPFL+KTY+MV+DP+T+A+VSWS +  SF+VW+PPEF+++LLP YFKHNNFSSFV
Sbjct: 46  ANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFV 105

Query: 67  RQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQS 126
           RQLNTYGFRK+D ++WEFANEGF+RG+ HLL+ I RRK +  H  + S+ Q+Q  S  Q 
Sbjct: 106 RQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQG 165

Query: 127 SNGSPFS--EPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGN 186
           S  +  S  E  +  LE +++ L ++   L  +L K  ++++  + ++Q L   L  M  
Sbjct: 166 SMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQ 225

Query: 187 EQKQLITMLAAQLQ 199
            Q+Q+++ LA  +Q
Sbjct: 226 RQQQIMSFLAKAVQ 239

[Close]

BLAST of MS014079 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 166.4 bits (420), Expect = 2.3e-41
Identity = 94/187 (50.27%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 12  PPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQLNT 71
           PPFL+KTY+MVDDP TN VVSWS    SFVVW+ PEF+K LLP YFKHNNFSSFVRQLNT
Sbjct: 26  PPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNT 85

Query: 72  YGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGHSHSQSSNGSP 131
           YGFRK+D ++WEFANEGF+RGR  LL++I RRKP  SH Q     QNQ  +  QSS+   
Sbjct: 86  YGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQ-----QNQQQTQVQSSSVGA 145

Query: 132 FSEPERQELENKIKTLNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQLIT 191
             E  +  +E +++ L ++   L  +L +  ++++  E Q+Q++  ++  M   Q+Q+++
Sbjct: 146 CVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMS 205

Query: 192 MLAAQLQ 199
            LA  +Q
Sbjct: 206 FLAKAVQ 205

The following BLAST results are available for this feature:

BLAST of MS014079 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149724.1	1.1e-101	97.49	heat stress transcription factor A-4c-like [Momordica charantia]	[more]
KAG7034870.1	1.7e-78	76.00	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6604741.1	1.1e-77	75.50	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022970770.1	1.1e-77	75.50	heat stress transcription factor A-4c-like [Cucurbita maxima]	[more]
XP_023534238.1	1.1e-77	75.50	heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS014079 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49403	4.3e-48	51.03	Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ...	[more]
Q93VB5	1.6e-47	52.02	Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q94J16	3.6e-47	54.64	Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9LQM7	6.9e-46	50.78	Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D ...	[more]
P41153	3.4e-45	50.00	Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1	[more]

BLAST of MS014079 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7W3	5.4e-102	97.49	heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1I3T7	5.4e-78	75.50	heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0KBI1	7.8e-77	76.00	HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 ...	[more]
A0A6J1G8J3	1.7e-76	74.50	heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5D3BGW6	9.6e-75	75.13	Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of MS014079 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18880.1	3.1e-49	51.03	heat shock transcription factor A4A	[more]
AT1G32330.1	4.9e-47	50.78	heat shock transcription factor A1D	[more]
AT5G45710.1	7.1e-46	47.74	winged-helix DNA-binding transcription factor family protein	[more]
AT4G17750.1	8.6e-44	47.42	heat shock factor 1	[more]
AT5G16820.1	2.3e-41	50.27	heat shock factor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 137..185
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..138
None	No IPR available	PANTHER	PTHR10015:SF312	HEAT STRESS TRANSCRIPTION FACTOR A-4D	coord: 7..199
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 65..77 score: 84.62 coord: 14..37 score: 50.48 coord: 52..64 score: 78.24
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 10..103 e-value: 1.6E-61 score: 220.4
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 14..103 e-value: 5.1E-32 score: 110.3
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 53..77
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 7..104 e-value: 4.1E-38 score: 132.0
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 7..199
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 10..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014079.1	MS014079.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding