MS014064 (gene) Bitter gourd (TR) v1

Overview


Name	MS014064
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	1-aminocyclopropane-1-carboxylate oxidase 1
Location	scaffold5: 302281 .. 303893 (+)
RNA-Seq Expression	MS014064
Synteny	MS014064

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCCCTGTGATCAACTTGGAGAAGCTGAACGGCCATGAGAGGGCAGCCATCATGGAGAAAATCAAGGACGCCTGTGAAAACTGGGGTTTCTTTGAGGTAACTTTAAAATAATTTCAATTATTCAATTGATTCATGTTCATATAAGACGAAAAAACGAAATTTCCCATGTCGGATTTCAGCTTCTGAACCACGGGATTGCGCCGGAGTTTCTGGACACTGTGGAGAGGCTAACTAAGCAACACTACAAGAAATGTATGGAGCAGCGGTTCCAAGAGCTCGTGTCCAGCAAGGGCCTCGACGCGGTCCAGGCCGAGGTCGACGATATCGACTGGGAAAGCACATTCTTCCTCCGCCATCTCCCAGTCTCCAACATCGCCGACCTCCCCGAACTCGACGACGAGTACAGGTAAGCATAATCCGTCGACCAGCTTAAAAGCTGAAGTTGATGGGTCATGTGTTTGAAATTTGTTGGATGTGTATTGGTTTCCAACGCAGGAGGGTAATGAAGGAATTTGCGGGGAAATTGGAGAAACTTGCGGAAGAGTTGTTGGAGTTGCTGTGTGAGAATCTTGGGCTGGAGAAAGGGTACCTGAAAAATGTGTTCTATGGGTCGAAGGGCCCCACCTTCGGCACCAAGGTCAGCAACTACCCGCCGTGCCCGAAGCCGGATCTGATCAAGGGACTCCGAGCCCACACCGACGCTGGTGGCATCATCCTTCTCTTCCAAGACCACAAGGTCAGCGGCCTCCAGCTGCTTAAGGACGGCCACTGGGTCGACGTTCCCCCGCTCCGCCACTCCATCGTCGTCAACATCGGCGACCAGCTCGAGGTAACCACCATCTACGGCTCATTCTTTGTAACAATCAAACGTTGTACTAACATTAACATGTACTTATCAGTCAGCACACATCTTTATGGTTGTCATTATTTGACAGGTTTTTTACTTTTTTGAATAAACATAAAAATGTTAAAAATTAAAAATCTCTTTGTCTATATATCTCTTCTAATTGTTAAAATTGTAATCGTCTTCAGCAAAAGATATATGCTAACGATTAAATATGTTATCGAAAAGTATGTTACTATACTGGACAAGAGGAGATTTTGCTAACCACAAATAAAAATCTGCTCTAATGTCACCAATGAATCCAAAAAGTTTAAATTGATGGGTTATGATAAATTTAATCGTGAGTCAGTAGTGACCGGTGGCAAAAATTTCTACTACCCAGTCAGTAGGGAAGTATCATTCTAGAGTATCTGGTTGAATTACAATTTCCTCTTTGGTTCATAGTAATTATAATCGCGGGATTTTGCAGGTGATAACGAACGGGAAGTACAAGAGTGTGGAGCACAGAGTGATAGCACAGGCAGATGGGGAAGGGAGGATGTCATTGGCATCATTCTACAACCCAGGGAGCGATGCAGTGATATACCCAGCCCCAACTCTGGTGGAGAAGGAGGCAGAAGAGAAAAATCAGGTCTACCCAAAATTTGTGTTTGAAGACTACATGAAGCTTTATACAGCTGTGAAGTTCCAGGCCAAGGAGCCAAGGTTTGAAGCCATGAAAGCTGTGGAAGCTAATGTCAAACTGGGTCCAATTGCAACTGTT

mRNA sequence

ATGGAGTTCCCTGTGATCAACTTGGAGAAGCTGAACGGCCATGAGAGGGCAGCCATCATGGAGAAAATCAAGGACGCCTGTGAAAACTGGGGTTTCTTTGAGCTTCTGAACCACGGGATTGCGCCGGAGTTTCTGGACACTGTGGAGAGGCTAACTAAGCAACACTACAAGAAATGTATGGAGCAGCGGTTCCAAGAGCTCGTGTCCAGCAAGGGCCTCGACGCGGTCCAGGCCGAGGTCGACGATATCGACTGGGAAAGCACATTCTTCCTCCGCCATCTCCCAGTCTCCAACATCGCCGACCTCCCCGAACTCGACGACGAGTACAGGAGGGTAATGAAGGAATTTGCGGGGAAATTGGAGAAACTTGCGGAAGAGTTGTTGGAGTTGCTGTGTGAGAATCTTGGGCTGGAGAAAGGGTACCTGAAAAATGTGTTCTATGGGTCGAAGGGCCCCACCTTCGGCACCAAGGTCAGCAACTACCCGCCGTGCCCGAAGCCGGATCTGATCAAGGGACTCCGAGCCCACACCGACGCTGGTGGCATCATCCTTCTCTTCCAAGACCACAAGGTCAGCGGCCTCCAGCTGCTTAAGGACGGCCACTGGGTCGACGTTCCCCCGCTCCGCCACTCCATCGTCGTCAACATCGGCGACCAGCTCGAGGTGATAACGAACGGGAAGTACAAGAGTGTGGAGCACAGAGTGATAGCACAGGCAGATGGGGAAGGGAGGATGTCATTGGCATCATTCTACAACCCAGGGAGCGATGCAGTGATATACCCAGCCCCAACTCTGGTGGAGAAGGAGGCAGAAGAGAAAAATCAGGTCTACCCAAAATTTGTGTTTGAAGACTACATGAAGCTTTATACAGCTGTGAAGTTCCAGGCCAAGGAGCCAAGGTTTGAAGCCATGAAAGCTGTGGAAGCTAATGTCAAACTGGGTCCAATTGCAACTGTT

Coding sequence (CDS)

ATGGAGTTCCCTGTGATCAACTTGGAGAAGCTGAACGGCCATGAGAGGGCAGCCATCATGGAGAAAATCAAGGACGCCTGTGAAAACTGGGGTTTCTTTGAGCTTCTGAACCACGGGATTGCGCCGGAGTTTCTGGACACTGTGGAGAGGCTAACTAAGCAACACTACAAGAAATGTATGGAGCAGCGGTTCCAAGAGCTCGTGTCCAGCAAGGGCCTCGACGCGGTCCAGGCCGAGGTCGACGATATCGACTGGGAAAGCACATTCTTCCTCCGCCATCTCCCAGTCTCCAACATCGCCGACCTCCCCGAACTCGACGACGAGTACAGGAGGGTAATGAAGGAATTTGCGGGGAAATTGGAGAAACTTGCGGAAGAGTTGTTGGAGTTGCTGTGTGAGAATCTTGGGCTGGAGAAAGGGTACCTGAAAAATGTGTTCTATGGGTCGAAGGGCCCCACCTTCGGCACCAAGGTCAGCAACTACCCGCCGTGCCCGAAGCCGGATCTGATCAAGGGACTCCGAGCCCACACCGACGCTGGTGGCATCATCCTTCTCTTCCAAGACCACAAGGTCAGCGGCCTCCAGCTGCTTAAGGACGGCCACTGGGTCGACGTTCCCCCGCTCCGCCACTCCATCGTCGTCAACATCGGCGACCAGCTCGAGGTGATAACGAACGGGAAGTACAAGAGTGTGGAGCACAGAGTGATAGCACAGGCAGATGGGGAAGGGAGGATGTCATTGGCATCATTCTACAACCCAGGGAGCGATGCAGTGATATACCCAGCCCCAACTCTGGTGGAGAAGGAGGCAGAAGAGAAAAATCAGGTCTACCCAAAATTTGTGTTTGAAGACTACATGAAGCTTTATACAGCTGTGAAGTTCCAGGCCAAGGAGCCAAGGTTTGAAGCCATGAAAGCTGTGGAAGCTAATGTCAAACTGGGTCCAATTGCAACTGTT

Protein sequence

MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV

Homology

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BLAST of MS014064 vs. NCBI nr
Match: XP_022149577.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia])

HSP 1 Score: 652.5 bits (1682), Expect = 1.9e-183
Identity = 318/319 (99.69%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM
Sbjct: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           E+RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL
Sbjct: 61  EKRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG
Sbjct: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD
Sbjct: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300
           GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR
Sbjct: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300

Query: 301 FEAMKAVEANVKLGPIATV 320
           FEAMKAVEANVKLGPIATV
Sbjct: 301 FEAMKAVEANVKLGPIATV 319

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BLAST of MS014064 vs. NCBI nr
Match: PON84482.1 (Oxoglutarate/iron-dependent dioxygenase [Trema orientale])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 278/316 (87.97%), Postives = 300/316 (94.94%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ER  IME+IKDACENWGFFEL+NHGI  E LDTVER+TK+HY+KCMEQ
Sbjct: 4   FPIINLEKLNGEERKNIMEQIKDACENWGFFELVNHGIPHELLDTVERMTKEHYRKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGLDAVQAEV+DIDWESTFFLRHLPVSNIA++P+LDDEYR++MK+FA KLEK
Sbjct: 64  RFKELVASKGLDAVQAEVNDIDWESTFFLRHLPVSNIAEIPDLDDEYRKIMKDFAQKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIV+NIGDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINIGDQLEVITNGKYKSVLHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDA+IYPAP LVEKEA+EKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE
Sbjct: 244 -RMSIASFYNPGSDAIIYPAPALVEKEAQEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIAT 319
           AMKAVEANVKLGPIAT
Sbjct: 304 AMKAVEANVKLGPIAT 318

[Close]

BLAST of MS014064 vs. NCBI nr
Match: PON41646.1 (Oxoglutarate/iron-dependent dioxygenase [Parasponia andersonii])

HSP 1 Score: 581.6 bits (1498), Expect = 4.0e-162
Identity = 278/316 (87.97%), Postives = 299/316 (94.62%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ER  IME+IKDACENWGFFEL+NHGI  E LDTVER+TK+HY+KCMEQ
Sbjct: 4   FPIINLEKLNGEERKNIMEQIKDACENWGFFELVNHGIPHELLDTVERMTKEHYRKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGLDAVQAEV+DIDWESTFFLRHLPVSNIA++P+LD EYR++MKEFA KLEK
Sbjct: 64  RFKELVASKGLDAVQAEVNDIDWESTFFLRHLPVSNIAEIPDLDGEYRKIMKEFAQKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIV+NIGDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINIGDQLEVITNGKYKSVLHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDA+IYPAP LVEKEA+EKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE
Sbjct: 244 -RMSIASFYNPGSDAIIYPAPALVEKEAQEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIAT 319
           AMKAVEANVKLGPIAT
Sbjct: 304 AMKAVEANVKLGPIAT 318

[Close]

BLAST of MS014064 vs. NCBI nr
Match: ACK43794.1 (1-aminocyclopropane-1-carboxylate oxidase [Momordica charantia])

HSP 1 Score: 576.6 bits (1485), Expect = 1.3e-160
Identity = 282/286 (98.60%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 34  ELLNHGIAPEFLDTVERLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFFLRH 93
           EL+NHGIAPEFLDT+ RLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFF RH
Sbjct: 49  ELVNHGIAPEFLDTLGRLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFFFRH 108

Query: 94  LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT 153
           LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT
Sbjct: 109 LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT 168

Query: 154 FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV 213
           FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV
Sbjct: 169 FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV 228

Query: 214 VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK 273
           VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK
Sbjct: 229 VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK 288

Query: 274 NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV 320
           NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV
Sbjct: 289 NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV 334

[Close]

BLAST of MS014064 vs. NCBI nr
Match: XP_021650334.1 (LOW QUALITY PROTEIN: 1-aminocyclopropane-1-carboxylate oxidase-like [Hevea brasiliensis])

HSP 1 Score: 576.2 bits (1484), Expect = 1.7e-160
Identity = 274/318 (86.16%), Postives = 293/318 (92.14%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           MEFPVINLEKLNG ERA  M KIKDACENWGFFELLNHGI PEFLDTVER+TK HY+KCM
Sbjct: 1   MEFPVINLEKLNGEERAVTMAKIKDACENWGFFELLNHGIEPEFLDTVERMTKGHYRKCM 60

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           EQRF+E+V+SKGL+ VQ E+ D+DWESTFFLRHLP SNIA +P+LDDEYR+VMKEFA KL
Sbjct: 61  EQRFKEMVASKGLEGVQTEIKDMDWESTFFLRHLPESNIAQVPDLDDEYRKVMKEFAAKL 120

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           EKLAEELL+LLCENLGLEKGYLK  FYGS GPTFGTKVSNYPPCPKPDL+KGLRAHTDAG
Sbjct: 121 EKLAEELLDLLCENLGLEKGYLKKAFYGSGGPTFGTKVSNYPPCPKPDLVKGLRAHTDAG 180

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           GIILLFQD KVSGLQLLKDG W+DVPPLRHSIV+N+GDQLEVITNGKYKSVEHRV+AQ D
Sbjct: 181 GIILLFQDDKVSGLQLLKDGQWIDVPPLRHSIVINLGDQLEVITNGKYKSVEHRVVAQTD 240

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300
           G  RMS+ASFYNPGSDAVIYPAP LVEKEAEEK QVYPKFVFEDYMKLY  +KFQAKEPR
Sbjct: 241 GT-RMSIASFYNPGSDAVIYPAPALVEKEAEEKKQVYPKFVFEDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAVEANVKLGPIAT 319
           FEAMKAVE NVKLGPIAT
Sbjct: 301 FEAMKAVETNVKLGPIAT 317

[Close]

BLAST of MS014064 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 5.0e-155
Identity = 265/317 (83.60%), Postives = 290/317 (91.48%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ER A ME IKDACENWGFFEL+NHGI  E +DTVE+LTK HYKKCMEQ
Sbjct: 4   FPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGL+AVQAEV D+DWESTFFLRHLPVSNI+++P+LDDEYR VM++FA +LEK
Sbjct: 64  RFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKRLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGP FGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIVVN+GDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEK-NQVYPKFVFEDYMKLYTAVKFQAKEPRF 302
            RMSLASFYNPGSDAVIYPAPTLVEKEA+++  QVYPKFVF+DYMKLY  +KFQAKEPRF
Sbjct: 244 -RMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKEPRF 303

Query: 303 EAMKAVEANVKLGPIAT 319
           EAMKA EA+VK  PIAT
Sbjct: 304 EAMKAREADVKSDPIAT 319

[Close]

BLAST of MS014064 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 8.5e-155
Identity = 259/317 (81.70%), Postives = 290/317 (91.48%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+I+L+K+NG ERAA ME IKDACENWGFFEL+NHGI  E +DTVE++TK HYKKCMEQ
Sbjct: 4   FPIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SK L+ VQAEV D+DWESTFFL+HLP+SNI+++P+LD+EYR VM++FA +LEK
Sbjct: 64  RFKELVASKALEGVQAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKRLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLKN FYGSKGP FGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIVVN+GDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRVIAQKDG- 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMSLASFYNPGSDAVIYPAP LVEKEAEE  QVYPKFVF+DYMKLY  +KFQAKEPRFE
Sbjct: 244 ARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIATV 320
           AMKA+E +VK+ PIATV
Sbjct: 304 AMKAMETDVKMDPIATV 319

[Close]

BLAST of MS014064 vs. ExPASy Swiss-Prot
Match: Q9MB94 (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.1e-154
Identity = 257/316 (81.33%), Postives = 290/316 (91.77%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLE LNG  R A MEKIKDACENWGFFEL++HGI  EFLDTVERLTK+HY++C+EQ
Sbjct: 4   FPIINLEGLNGEGRKATMEKIKDACENWGFFELVSHGIPTEFLDTVERLTKEHYRQCLEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGL+AV+ EV+D+DWESTF+LRHLP SNI+++P+L+D+YR VMKEFA KLEK
Sbjct: 64  RFKELVASKGLEAVKTEVNDMDWESTFYLRHLPKSNISEVPDLEDQYRNVMKEFALKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAE+LL+LLCENLGLEKGYLK  FYG+ GPTFGTKVSNYPPCP P+LIKGLRAHTDAGG+
Sbjct: 124 LAEQLLDLLCENLGLEKGYLKKAFYGTNGPTFGTKVSNYPPCPNPELIKGLRAHTDAGGL 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIV+N+GDQLEVITNGKY+SVEHRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGKYRSVEHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLY  +KFQ KEPRFE
Sbjct: 244 -RMSIASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYAGLKFQPKEPRFE 303

Query: 303 AMKAVEANVKLGPIAT 319
           AMKAVE N+ L PIAT
Sbjct: 304 AMKAVETNISLVPIAT 318

[Close]

BLAST of MS014064 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 2.7e-153
Identity = 251/317 (79.18%), Postives = 293/317 (92.43%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FPVI++EKLNG ERA  MEKIKDACENWGFFEL+NHGI+ E +DTVERLTK+HY KCMEQ
Sbjct: 4   FPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+E+V++KGL+AVQ+E++D+DWESTFFLRHLPVSNI+++P+L+ ++R+ MKEFA KLEK
Sbjct: 64  RFKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEFAEKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAE+LL+LLCEN+GLEKGYLK  FYGSKGPTFGTKVSNYPPCP+P+LIKGLRAHTDAGGI
Sbjct: 124 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD+KVSGLQLLKDG W+DVPP++HSIV+NIGDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHRVIAQPDG- 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDAV+YPAP LV+KE +++ QVYPKFVFEDYMKLY  +KFQAKEPRFE
Sbjct: 244 NRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIATV 320
           AMKA+E  V LGPIAT+
Sbjct: 304 AMKAMENAVNLGPIATI 319

[Close]

BLAST of MS014064 vs. ExPASy Swiss-Prot
Match: Q08508 (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Petunia hybrida OX=4102 GN=ACO4 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 4.6e-153
Identity = 257/317 (81.07%), Postives = 286/317 (90.22%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLE L G ER A ME IKDACENWGFFEL+NHGI  E +DTVE+ TK HYKKCMEQ
Sbjct: 4   FPIINLENLCGAERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKFTKGHYKKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGL+AVQAEV D+DWESTFFLRHLPVSNI+++P+LDDEYR VM++FA +LEK
Sbjct: 64  RFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKRLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGP FGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKD  W+DVPP+RHSIV+N+GDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDDQWIDVPPMRHSIVINLGDQLEVITNGKYKSVPHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMSLASFYNP SDAVIYPAP LVE++AEE  Q+YPKFVF+DYMKLY  +KFQAKEPRFE
Sbjct: 244 -RMSLASFYNPASDAVIYPAPALVERDAEENKQIYPKFVFDDYMKLYARLKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIATV 320
           AMKA+EA+VK+ P+ATV
Sbjct: 304 AMKAMEADVKIDPVATV 319

[Close]

BLAST of MS014064 vs. ExPASy TrEMBL
Match: A0A6J1D8D7 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LOC111017974 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.0e-184
Identity = 318/319 (99.69%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM
Sbjct: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           E+RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL
Sbjct: 61  EKRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG
Sbjct: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD
Sbjct: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300
           GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR
Sbjct: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300

Query: 301 FEAMKAVEANVKLGPIATV 320
           FEAMKAVEANVKLGPIATV
Sbjct: 301 FEAMKAVEANVKLGPIATV 319

[Close]

BLAST of MS014064 vs. ExPASy TrEMBL
Match: A0A2P5EG33 (Oxoglutarate/iron-dependent dioxygenase OS=Trema orientale OX=63057 GN=TorACO4 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.7e-163
Identity = 278/316 (87.97%), Postives = 300/316 (94.94%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ER  IME+IKDACENWGFFEL+NHGI  E LDTVER+TK+HY+KCMEQ
Sbjct: 4   FPIINLEKLNGEERKNIMEQIKDACENWGFFELVNHGIPHELLDTVERMTKEHYRKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGLDAVQAEV+DIDWESTFFLRHLPVSNIA++P+LDDEYR++MK+FA KLEK
Sbjct: 64  RFKELVASKGLDAVQAEVNDIDWESTFFLRHLPVSNIAEIPDLDDEYRKIMKDFAQKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIV+NIGDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINIGDQLEVITNGKYKSVLHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDA+IYPAP LVEKEA+EKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE
Sbjct: 244 -RMSIASFYNPGSDAIIYPAPALVEKEAQEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIAT 319
           AMKAVEANVKLGPIAT
Sbjct: 304 AMKAVEANVKLGPIAT 318

[Close]

BLAST of MS014064 vs. ExPASy TrEMBL
Match: A0A2P5AYT2 (Oxoglutarate/iron-dependent dioxygenase OS=Parasponia andersonii OX=3476 GN=PanACO4 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 278/316 (87.97%), Postives = 299/316 (94.62%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ER  IME+IKDACENWGFFEL+NHGI  E LDTVER+TK+HY+KCMEQ
Sbjct: 4   FPIINLEKLNGEERKNIMEQIKDACENWGFFELVNHGIPHELLDTVERMTKEHYRKCMEQ 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+ELV+SKGLDAVQAEV+DIDWESTFFLRHLPVSNIA++P+LD EYR++MKEFA KLEK
Sbjct: 64  RFKELVASKGLDAVQAEVNDIDWESTFFLRHLPVSNIAEIPDLDGEYRKIMKEFAQKLEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           LAEELL+LLCENLGLEKGYLK  FYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI
Sbjct: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG W+DVPP+RHSIV+NIGDQLEVITNGKYKSV HRVIAQ DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINIGDQLEVITNGKYKSVLHRVIAQTDGT 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 302
            RMS+ASFYNPGSDA+IYPAP LVEKEA+EKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE
Sbjct: 244 -RMSIASFYNPGSDAIIYPAPALVEKEAQEKNQVYPKFVFEDYMKLYTAVKFQAKEPRFE 303

Query: 303 AMKAVEANVKLGPIAT 319
           AMKAVEANVKLGPIAT
Sbjct: 304 AMKAVEANVKLGPIAT 318

[Close]

BLAST of MS014064 vs. ExPASy TrEMBL
Match: B7U9D1 (1-aminocyclopropane-1-carboxylate oxidase OS=Momordica charantia OX=3673 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.3e-161
Identity = 282/286 (98.60%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 34  ELLNHGIAPEFLDTVERLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFFLRH 93
           EL+NHGIAPEFLDT+ RLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFF RH
Sbjct: 49  ELVNHGIAPEFLDTLGRLTKQHYKKCMEQRFQELVSSKGLDAVQAEVDDIDWESTFFFRH 108

Query: 94  LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT 153
           LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT
Sbjct: 109 LPVSNIADLPELDDEYRRVMKEFAGKLEKLAEELLELLCENLGLEKGYLKNVFYGSKGPT 168

Query: 154 FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV 213
           FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV
Sbjct: 169 FGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIV 228

Query: 214 VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK 273
           VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK
Sbjct: 229 VNIGDQLEVITNGKYKSVEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEK 288

Query: 274 NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV 320
           NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV
Sbjct: 289 NQVYPKFVFEDYMKLYTAVKFQAKEPRFEAMKAVEANVKLGPIATV 334

[Close]

BLAST of MS014064 vs. ExPASy TrEMBL
Match: A0A6J1I4V9 (1-aminocyclopropane-1-carboxylate oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469659 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 280/311 (90.03%), Postives = 290/311 (93.25%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           MEFP+INLEKLNG ERA I+EKIKDACENWGFFELLNHGI PEFLD VERLTKQHYKKCM
Sbjct: 1   MEFPIINLEKLNGEERAPILEKIKDACENWGFFELLNHGIEPEFLDKVERLTKQHYKKCM 60

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           EQRF+ELV SKGLD VQAEV+D+DWESTFFLRHLPVSNIADLP+LDDEYR VMKEFA KL
Sbjct: 61  EQRFKELVESKGLDGVQAEVNDLDWESTFFLRHLPVSNIADLPDLDDEYRAVMKEFAAKL 120

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           EKLAE LL+LLCENLGL KGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG
Sbjct: 121 EKLAEVLLDLLCENLGLPKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           GIILLFQD KVSGLQLLKD  WVDVPPLRHSIVVNIGDQLEVITNGKYKSV HRVIAQ D
Sbjct: 181 GIILLFQDDKVSGLQLLKDDQWVDVPPLRHSIVVNIGDQLEVITNGKYKSVLHRVIAQRD 240

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQV-YPKFVFEDYMKLYTAVKFQAKEP 300
           GEGRMSLASFYNPGSDAVIYPAP LV KE EEKN+V YPKFVFEDYMKLY  +KFQAKEP
Sbjct: 241 GEGRMSLASFYNPGSDAVIYPAPALVGKEGEEKNEVLYPKFVFEDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKAVEAN 311
           RFEAMKAVE+N
Sbjct: 301 RFEAMKAVESN 311

[Close]

BLAST of MS014064 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 537.7 bits (1384), Expect = 6.2e-153
Identity = 249/319 (78.06%), Postives = 293/319 (91.85%), Query Frame = 0


Query: 3   FPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCMEQ 62
           FP+INLEKLNG ERA  MEKIKDACENWGFFE +NHGI+ E LD VE++TK+HYKKCME+
Sbjct: 4   FPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCMEE 63

Query: 63  RFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKLEK 122
           RF+E + ++GLD++++EV+D+DWESTF+L+HLPVSNI+D+P+LDD+YR +MK+FAGK+EK
Sbjct: 64  RFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGKIEK 123

Query: 123 LAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 182
           L+EELL+LLCENLGLEKGYLK VFYGSK PTFGTKVSNYPPCP PDL+KGLRAHTDAGGI
Sbjct: 124 LSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAGGI 183

Query: 183 ILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQADGE 242
           ILLFQD KVSGLQLLKDG WVDVPP++HSIVVN+GDQLEVITNGKYKSVEHRV++Q DGE
Sbjct: 184 ILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQTDGE 243

Query: 243 GRMSLASFYNPGSDAVIYPAPTLVEKEAE-EKNQVYPKFVFEDYMKLYTAVKFQAKEPRF 302
           GRMS+ASFYNPGSD+VI+PAP L+ KEAE EK + YP+FVFEDYMKLY+AVKFQAKEPRF
Sbjct: 244 GRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKEPRF 303

Query: 303 EAMKAVEANV--KLGPIAT 319
           EAMKA+E  V   +GP+AT
Sbjct: 304 EAMKAMETTVANNVGPLAT 322

[Close]

BLAST of MS014064 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-132
Identity = 218/319 (68.34%), Postives = 266/319 (83.39%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           ++FPVI+L KLNG ER   M  I DAC+NWGFFEL+NHG+  + +D +ER+TK+HYKK M
Sbjct: 5   IKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHM 64

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           EQ+F+E++ SKGLD ++ EV+D+DWESTF+L HLP SN+ D+P++ +EYR  MK+F  +L
Sbjct: 65  EQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRL 124

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           E LAEELL+LLCENLGLEKGYLK VF+G+ GPTF TK+SNYPPCPKP++IKGLRAHTDAG
Sbjct: 125 EILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAG 184

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           G+ILLFQD KVSGLQLLKDG WVDVPPL+HSIV+N+GDQLEVITNGKYKSV HRV+ Q +
Sbjct: 185 GLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKE 244

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300
           G  RMS+ASFYNPGSDA I PA +LV+K+++     YP FVF+DYMKLY  +KFQAKEPR
Sbjct: 245 G-NRMSIASFYNPGSDAEISPATSLVDKDSK-----YPSFVFDDYMKLYAGLKFQAKEPR 304

Query: 301 FEAMKAVEANVKLGPIATV 320
           FEAMK  EA   L P+A V
Sbjct: 305 FEAMKNAEAAADLNPVAVV 317

[Close]

BLAST of MS014064 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 466.5 bits (1199), Expect = 1.8e-131
Identity = 216/319 (67.71%), Postives = 261/319 (81.82%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           M+FPV++L KLNG ER   M  I +ACENWGFFE++NHG+  + +D +E++TK HYK C 
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           EQ+F +++ SKGLD ++ EV+D+DWESTF++RHLP SN+ D+ ++ DEYR  MK+F  +L
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRL 124

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
           E LAE+LL+LLCENLGLEKGYLK VF+G+KGPTFGTKVSNYPPCPKP++IKGLRAHTDAG
Sbjct: 125 ENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAG 184

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKSVEHRVIAQAD 240
           GIILLFQD KVSGLQLLKDG W+DVPPL HSIV+N+GDQLEVITNGKYKSV HRV+ Q +
Sbjct: 185 GIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQE 244

Query: 241 GEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYTAVKFQAKEPR 300
           G  RMS+ASFYNPGSDA I PA +LVEK++E     YP FVF+DYMKLY  VKFQ KEPR
Sbjct: 245 G-NRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMKLYAGVKFQPKEPR 304

Query: 301 FEAMKAVEANVKLGPIATV 320
           F AMK   A  +L P A V
Sbjct: 305 FAAMKNASAVTELNPTAAV 317

[Close]

BLAST of MS014064 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 144/307 (46.91%), Postives = 204/307 (66.45%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           ME PVI+  +L+G +R+  M  +  AC+ WGFF + NHGI  E ++ V+++   HY++ +
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EQRFQELVSSKGLDAVQAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKEFAGKL 120
           +++F +    K L   + +  D DWES+FF+ H P SNI  +P + +E  + M E+  +L
Sbjct: 69  KEKFYQSEMVKALS--EGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQL 128

Query: 121 EKLAEELLELLCENLGLEKGYLKNVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 180
            K AE L +L+CENLGL++  + N F G KGP FGTKV+ YP CP+P+L++GLR HTDAG
Sbjct: 129 HKFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAG 188

Query: 181 GIILLFQDHKVSGLQLLKDGHWVDVPPLR-HSIVVNIGDQLEVITNGKYKSVEHRVIAQA 240
           GIILL QD +V GL+  KDG WV +PP + ++I VN GDQLE+++NG+YKSV HRV+   
Sbjct: 189 GIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVK 248

Query: 241 DGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPK-FVFEDYMKLYTAVKFQAKE 300
            G  R+S+A+FYNP  DA+I PAP L          +YP  + F+DY+KLY+  KF  K 
Sbjct: 249 HG-SRLSIATFYNPAGDAIISPAPKL----------LYPSGYRFQDYLKLYSTTKFGDKG 302

Query: 301 PRFEAMK 306
           PR E MK
Sbjct: 309 PRLETMK 302

[Close]

BLAST of MS014064 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.3e-77
Identity = 148/317 (46.69%), Postives = 204/317 (64.35%), Query Frame = 0


Query: 1   MEFPVINLEKLNGHERAAIMEKIKDACENWGFFELLNHGIAPEFLDTVERLTKQHYKKCM 60
           M  PVI+  KLNG ER   + +I  ACE WGFF+L+NHGI  E L+ V++L+   YK   
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EQRFQELVSSKGLDAV-----QAEVDDIDWESTFFLRHLPVSNIADLPELDDEYRRVMKE 120
           E+ F+     K L+ +       +++++DWE  F L         +  E     +  M E
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLD------HNQNEWPSNIKETMGE 120

Query: 121 FAGKLEKLAEELLELLCENLGLEKGYLKNVFY-----GSKGPTFGTKVSNYPPCPKPDLI 180
           +  ++ KLA +++E++ ENLGL KGY+K  F      G +   FGTKVS+YPPCP P+L+
Sbjct: 121 YREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELV 180

Query: 181 KGLRAHTDAGGIILLFQDHKVSGLQLLKDGHWVDVPPLRHSIVVNIGDQLEVITNGKYKS 240
            GLRAHTDAGG++LLFQD +  GLQ+LKDG W+DV PL ++IV+N GDQ+EV++NG+YKS
Sbjct: 181 NGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKS 240

Query: 241 VEHRVIAQADGEGRMSLASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYT 300
             HRV+A+ +G  R S+ASFYNP   A I PA    E+ +E+K   YPKFVF DYM +Y 
Sbjct: 241 AWHRVLAREEG-NRRSIASFYNPSYKAAIGPAAVAEEEGSEKK---YPKFVFGDYMDVYA 300

Query: 301 AVKFQAKEPRFEAMKAV 308
             KF  KEPRF A+K++
Sbjct: 301 NQKFMPKEPRFLAVKSL 307

The following BLAST results are available for this feature:

BLAST of MS014064 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149577.1	1.9e-183	99.69	1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia]	[more]
PON84482.1	1.4e-162	87.97	Oxoglutarate/iron-dependent dioxygenase [Trema orientale]	[more]
PON41646.1	4.0e-162	87.97	Oxoglutarate/iron-dependent dioxygenase [Parasponia andersonii]	[more]
ACK43794.1	1.3e-160	98.60	1-aminocyclopropane-1-carboxylate oxidase [Momordica charantia]	[more]
XP_021650334.1	1.7e-160	86.16	LOW QUALITY PROTEIN: 1-aminocyclopropane-1-carboxylate oxidase-like [Hevea brasi...	[more]

BLAST of MS014064 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q08507	5.0e-155	83.60	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P...	[more]
Q08506	8.5e-155	81.70	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P...	[more]
Q9MB94	1.1e-154	81.33	1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 ...	[more]
P31237	2.7e-153	79.18	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
Q08508	4.6e-153	81.07	1-aminocyclopropane-1-carboxylate oxidase 4 OS=Petunia hybrida OX=4102 GN=ACO4 P...	[more]

BLAST of MS014064 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8D7	9.0e-184	99.69	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A2P5EG33	6.7e-163	87.97	Oxoglutarate/iron-dependent dioxygenase OS=Trema orientale OX=63057 GN=TorACO4 P...	[more]
A0A2P5AYT2	2.0e-162	87.97	Oxoglutarate/iron-dependent dioxygenase OS=Parasponia andersonii OX=3476 GN=PanA...	[more]
B7U9D1	6.3e-161	98.60	1-aminocyclopropane-1-carboxylate oxidase OS=Momordica charantia OX=3673 GN=ACO1...	[more]
A0A6J1I4V9	1.1e-160	90.03	1-aminocyclopropane-1-carboxylate oxidase 1-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of MS014064 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05010.1	6.2e-153	78.06	ethylene-forming enzyme	[more]
AT1G12010.1	6.0e-132	68.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G62380.1	1.8e-131	67.71	ACC oxidase 2	[more]
AT2G19590.1	2.6e-82	46.91	ACC oxidase 1	[more]
AT1G77330.1	4.3e-77	46.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 113..133
None	No IPR available	PANTHER	PTHR47990:SF87	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 2..307
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..307
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..305
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 4..63 e-value: 1.1E-11 score: 45.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..319 e-value: 5.1E-102 score: 343.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 158..253 e-value: 8.3E-27 score: 93.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 152..253 score: 13.109252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014064.1	MS014064.1	mRNA