MS014030 (gene) Bitter gourd (TR) v1

Overview
NameMS014030
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP-dependent zinc metalloprotease FtsH
Locationscaffold5: 100509 .. 103011 (+)
RNA-Seq ExpressionMS014030
SyntenyMS014030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGATATGGATTCGTCATCAGCATGTCTGATAGGACGTGGTTCTTCCCACTGTGCTAAACTAAAATTCTGCAAAGATTTCTATGGTGTGAATATTGTCCCTTCTCCTACTCTTCTTTCGTTGAGTAAGGTTAGCAGAGCAGCTATAGTAAGGGCATCTCTGGGACATGGGAAACATGAAGGAAGACGAGGGTTTCTAAAGTTGGTTGGAAATGTGGGATTTGGTGTTCCTGCTCTACTGGGAAGTGGGAGGGCTAATGCTGAAGAACAAGGGGTTTCTTCATCAAGGATGTCTTATTCTAGATTCTTGGAGTATTTGGACAAGGATAGAGTGACAAGAGTGGACCTGTACGAGAATGGAACTATAGCTATTGTTGAAGCTATCTCCCCGGAATTGGGGAATCGGTTGCAGCGAGTTCGTGTTCAACTTCCAGGTCTGAGTCAGGAGCTTCTTCAGAAGCTCAGGGAGAAAAACATAGACTTTGCAGCTCATAATGCTCAAGAAGAATCTGGTTCTGCTTTATTCAACTTGATTGGGAACCTAGCTTTTCCACTGATCTTGATTGGGGGGCTATTTTTACTCTCAAGGCGTTCATCTGGGGGAATGGGTGGTCCTGGTGGGCCAGGGTTTCCCCTTGGCATTGGCCAGTCAAAAGCTAAGTTTCAAATGGAGCCAAACACTGGAGTGAATTTTGATGATGTGGCTGGTGTTGATGAAGCGAAACAGGACTTCATGGAGGTCGTAGAGTTTTTGAAGAAGCCCGAGAGGTTTACTGCAGTTGGAGCTCGGATTCCTAAAGGTGTTCTTCTTATTGGTCCTCCTGGAACTGGTAAGACTCTGCTGGCAAAAGCAATTGCTGGTGAAGCTGGTGTTCCATTTTTCTCCATATCAGGTTCTGAGTTTGTTGAGATGTTTGTTGGTGTTGGTGCATCTCGAGTTCGCGACCTATTCAAGAAGGCCAAAGAGAATGCTCCTTGCATTGTGTTTGTTGATGAAATTGATGCGGTCGGGCGACAAAGAGGAGCTGGGATTGGTGGAGGGAATGATGAAAGAGAACAGACACTCAACCAACTTTTAACCGAAATGGATGGTTTCGAGGGTAATACCGGTATCATTGTAATTGCTGCCACCAACAGGGCAGATATTCTCGACTCTGCCTTACTAAGACCCGGACGTTTTGACAGACAAGTAAATACCATAGCTTCTGTTGTTCCTCGTTATTGATTTTTACATTATTGACTTACTCTAGCACTCTGATGCCTGCAGGTAACAGTTGATGTCCCAGACATACGGGGAAGAACAGATATTTTAAAAGTTCATGCTAGCAATAAGAAGTTTGATACTGATGTTTCTCTTGATGTCATTGCAATGAGAACACCTGGTTTTAGTGGAGCTGATCTTGCAAATCTCTTAAACGAGGCCGCTATACTCGCTGGTCGGCGTGGGAAGACTGCAATTTCATCTAAAGAGATTGATGATTCGATTGATAGGATCGTAGCTGGAATGGAAGGAACGATAATGACAGATGGGAAGAGTAAAAGTCTTGTGGCATATCATGAAGTTGGGCATGCCATTTGTGGGTGAGTAGAATAATAGTTGCTTGTCTTGTTTACGAGGAATCCTCCATATTAAACTGCTGGAAAAACTTTTCTTTTTCTTTTGTTTGAACTTTAGACTTGCTAGGCAGATGAAGTTAACTCAAAAAGTAATATCTTTGATGCAGGACTCTGACTCCAGGACATGATGCTGTTCAAAAAGTTACCTTAGTTCCTCGTGGGCAAGCACGGGGTCTGACATGGTTTATTCCTGCAGATGATCCGACCTTGATTTCCAAGCAGCAGCTCTTTGCAAGAATTGTAGGTGGACTTGGTGGTAGAGCTGCAGAGGAAGTCATATTCGGCGAGCCCGAGGTGACCACTGGTGCAGTTGGCGATTTGCAGCAGATTACTGGTTTGGCAAAACAGGTATGTAGAACGTCTCCATTCTGCCTGCTAGTCACTCTCTGTAAAGGGCTATACTTCAAGGAATGAATTAAAGATTCTCCCACATTCATCAGTTGTATATGGGGACTGGGGGGTGGTAAATGGGACCCTTTTTTGTTAACTCTCTGCTTTTTCCACTAAAAAAATTCAATGAGATTAAGGTAATTTCAACCTGTTTGCAGATGGTAACCACATTTGGGATGTCTGAAATTGGACCATGGTCACTTATGGACCCATCAGCTCAGAGTGCTGATGTCATTATGAGAATGATGGCGAGGAATTCAATGTCGGAGAAGCTCGCCGAAGATATAGACATGGCAATCAAGAGGATATCAGATGAAGCTTATGAAATTGCATTAGCCCATATTAGGAACAACCGTGAGGCCATTGACAAGATAGTGGAAATCCTACTCGAGAAGGAAACCATGTCTGGAGATGAATTCCGAGCTATTCTCTCGGAGTTCGTCGAAATCCCAGTTGAAAATCGGGTTCCTCCTCCAGCACCCGCACCAGTTTCAGCT

mRNA sequence

ATAGATATGGATTCGTCATCAGCATGTCTGATAGGACGTGGTTCTTCCCACTGTGCTAAACTAAAATTCTGCAAAGATTTCTATGGTGTGAATATTGTCCCTTCTCCTACTCTTCTTTCGTTGAGTAAGGTTAGCAGAGCAGCTATAGTAAGGGCATCTCTGGGACATGGGAAACATGAAGGAAGACGAGGGTTTCTAAAGTTGGTTGGAAATGTGGGATTTGGTGTTCCTGCTCTACTGGGAAGTGGGAGGGCTAATGCTGAAGAACAAGGGGTTTCTTCATCAAGGATGTCTTATTCTAGATTCTTGGAGTATTTGGACAAGGATAGAGTGACAAGAGTGGACCTGTACGAGAATGGAACTATAGCTATTGTTGAAGCTATCTCCCCGGAATTGGGGAATCGGTTGCAGCGAGTTCGTGTTCAACTTCCAGGTCTGAGTCAGGAGCTTCTTCAGAAGCTCAGGGAGAAAAACATAGACTTTGCAGCTCATAATGCTCAAGAAGAATCTGGTTCTGCTTTATTCAACTTGATTGGGAACCTAGCTTTTCCACTGATCTTGATTGGGGGGCTATTTTTACTCTCAAGGCGTTCATCTGGGGGAATGGGTGGTCCTGGTGGGCCAGGGTTTCCCCTTGGCATTGGCCAGTCAAAAGCTAAGTTTCAAATGGAGCCAAACACTGGAGTGAATTTTGATGATGTGGCTGGTGTTGATGAAGCGAAACAGGACTTCATGGAGGTCGTAGAGTTTTTGAAGAAGCCCGAGAGGTTTACTGCAGTTGGAGCTCGGATTCCTAAAGGTGTTCTTCTTATTGGTCCTCCTGGAACTGGTAAGACTCTGCTGGCAAAAGCAATTGCTGGTGAAGCTGGTGTTCCATTTTTCTCCATATCAGGTTCTGAGTTTGTTGAGATGTTTGTTGGTGTTGGTGCATCTCGAGTTCGCGACCTATTCAAGAAGGCCAAAGAGAATGCTCCTTGCATTGTGTTTGTTGATGAAATTGATGCGGTCGGGCGACAAAGAGGAGCTGGGATTGGTGGAGGGAATGATGAAAGAGAACAGACACTCAACCAACTTTTAACCGAAATGGATGGTTTCGAGGGTAATACCGGTATCATTGTAATTGCTGCCACCAACAGGGCAGATATTCTCGACTCTGCCTTACTAAGACCCGGACGTTTTGACAGACAAGTAACAGTTGATGTCCCAGACATACGGGGAAGAACAGATATTTTAAAAGTTCATGCTAGCAATAAGAAGTTTGATACTGATGTTTCTCTTGATGTCATTGCAATGAGAACACCTGGTTTTAGTGGAGCTGATCTTGCAAATCTCTTAAACGAGGCCGCTATACTCGCTGGTCGGCGTGGGAAGACTGCAATTTCATCTAAAGAGATTGATGATTCGATTGATAGGATCGTAGCTGGAATGGAAGGAACGATAATGACAGATGGGAAGAGTAAAAGTCTTGTGGCATATCATGAAGTTGGGCATGCCATTTGTGGGACTCTGACTCCAGGACATGATGCTGTTCAAAAAGTTACCTTAGTTCCTCGTGGGCAAGCACGGGGTCTGACATGGTTTATTCCTGCAGATGATCCGACCTTGATTTCCAAGCAGCAGCTCTTTGCAAGAATTGTAGGTGGACTTGGTGGTAGAGCTGCAGAGGAAGTCATATTCGGCGAGCCCGAGGTGACCACTGGTGCAGTTGGCGATTTGCAGCAGATTACTGGTTTGGCAAAACAGGTATGTAGAACGTCTCCATTCTGCCTGCTAGTAATTTCAACCTGTTTGCAGATGGTAACCACATTTGGGATGTCTGAAATTGGACCATGGTCACTTATGGACCCATCAGCTCAGAGTGCTGATGTCATTATGAGAATGATGGCGAGGAATTCAATGTCGGAGAAGCTCGCCGAAGATATAGACATGGCAATCAAGAGGATATCAGATGAAGCTTATGAAATTGCATTAGCCCATATTAGGAACAACCGTGAGGCCATTGACAAGATAGTGGAAATCCTACTCGAGAAGGAAACCATGTCTGGAGATGAATTCCGAGCTATTCTCTCGGAGTTCGTCGAAATCCCAGTTGAAAATCGGGTTCCTCCTCCAGCACCCGCACCAGTTTCAGCT

Coding sequence (CDS)

ATAGATATGGATTCGTCATCAGCATGTCTGATAGGACGTGGTTCTTCCCACTGTGCTAAACTAAAATTCTGCAAAGATTTCTATGGTGTGAATATTGTCCCTTCTCCTACTCTTCTTTCGTTGAGTAAGGTTAGCAGAGCAGCTATAGTAAGGGCATCTCTGGGACATGGGAAACATGAAGGAAGACGAGGGTTTCTAAAGTTGGTTGGAAATGTGGGATTTGGTGTTCCTGCTCTACTGGGAAGTGGGAGGGCTAATGCTGAAGAACAAGGGGTTTCTTCATCAAGGATGTCTTATTCTAGATTCTTGGAGTATTTGGACAAGGATAGAGTGACAAGAGTGGACCTGTACGAGAATGGAACTATAGCTATTGTTGAAGCTATCTCCCCGGAATTGGGGAATCGGTTGCAGCGAGTTCGTGTTCAACTTCCAGGTCTGAGTCAGGAGCTTCTTCAGAAGCTCAGGGAGAAAAACATAGACTTTGCAGCTCATAATGCTCAAGAAGAATCTGGTTCTGCTTTATTCAACTTGATTGGGAACCTAGCTTTTCCACTGATCTTGATTGGGGGGCTATTTTTACTCTCAAGGCGTTCATCTGGGGGAATGGGTGGTCCTGGTGGGCCAGGGTTTCCCCTTGGCATTGGCCAGTCAAAAGCTAAGTTTCAAATGGAGCCAAACACTGGAGTGAATTTTGATGATGTGGCTGGTGTTGATGAAGCGAAACAGGACTTCATGGAGGTCGTAGAGTTTTTGAAGAAGCCCGAGAGGTTTACTGCAGTTGGAGCTCGGATTCCTAAAGGTGTTCTTCTTATTGGTCCTCCTGGAACTGGTAAGACTCTGCTGGCAAAAGCAATTGCTGGTGAAGCTGGTGTTCCATTTTTCTCCATATCAGGTTCTGAGTTTGTTGAGATGTTTGTTGGTGTTGGTGCATCTCGAGTTCGCGACCTATTCAAGAAGGCCAAAGAGAATGCTCCTTGCATTGTGTTTGTTGATGAAATTGATGCGGTCGGGCGACAAAGAGGAGCTGGGATTGGTGGAGGGAATGATGAAAGAGAACAGACACTCAACCAACTTTTAACCGAAATGGATGGTTTCGAGGGTAATACCGGTATCATTGTAATTGCTGCCACCAACAGGGCAGATATTCTCGACTCTGCCTTACTAAGACCCGGACGTTTTGACAGACAAGTAACAGTTGATGTCCCAGACATACGGGGAAGAACAGATATTTTAAAAGTTCATGCTAGCAATAAGAAGTTTGATACTGATGTTTCTCTTGATGTCATTGCAATGAGAACACCTGGTTTTAGTGGAGCTGATCTTGCAAATCTCTTAAACGAGGCCGCTATACTCGCTGGTCGGCGTGGGAAGACTGCAATTTCATCTAAAGAGATTGATGATTCGATTGATAGGATCGTAGCTGGAATGGAAGGAACGATAATGACAGATGGGAAGAGTAAAAGTCTTGTGGCATATCATGAAGTTGGGCATGCCATTTGTGGGACTCTGACTCCAGGACATGATGCTGTTCAAAAAGTTACCTTAGTTCCTCGTGGGCAAGCACGGGGTCTGACATGGTTTATTCCTGCAGATGATCCGACCTTGATTTCCAAGCAGCAGCTCTTTGCAAGAATTGTAGGTGGACTTGGTGGTAGAGCTGCAGAGGAAGTCATATTCGGCGAGCCCGAGGTGACCACTGGTGCAGTTGGCGATTTGCAGCAGATTACTGGTTTGGCAAAACAGGTATGTAGAACGTCTCCATTCTGCCTGCTAGTAATTTCAACCTGTTTGCAGATGGTAACCACATTTGGGATGTCTGAAATTGGACCATGGTCACTTATGGACCCATCAGCTCAGAGTGCTGATGTCATTATGAGAATGATGGCGAGGAATTCAATGTCGGAGAAGCTCGCCGAAGATATAGACATGGCAATCAAGAGGATATCAGATGAAGCTTATGAAATTGCATTAGCCCATATTAGGAACAACCGTGAGGCCATTGACAAGATAGTGGAAATCCTACTCGAGAAGGAAACCATGTCTGGAGATGAATTCCGAGCTATTCTCTCGGAGTTCGTCGAAATCCCAGTTGAAAATCGGGTTCCTCCTCCAGCACCCGCACCAGTTTCAGCT

Protein sequence

IDMDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGRRGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA
Homology
BLAST of MS014030 vs. NCBI nr
Match: XP_022149706.1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 692/709 (97.60%), Postives = 692/709 (97.60%), Query Frame = 0

Query: 3   MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR 62
           MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR
Sbjct: 1   MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR 60

Query: 63  RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI 122
           RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI
Sbjct: 61  RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI 120

Query: 123 AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA 182
           AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA
Sbjct: 121 AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA 180

Query: 183 FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ 242
           FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ
Sbjct: 181 FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ 240

Query: 243 DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV 302
           DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV
Sbjct: 241 DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV 300

Query: 303 EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM 362
           EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM
Sbjct: 301 EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM 360

Query: 363 DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT 422
           DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT
Sbjct: 361 DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT 420

Query: 423 DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT 482
           DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT
Sbjct: 421 DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT 480

Query: 483 DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF 542
           DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF
Sbjct: 481 DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF 540

Query: 543 ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMVTT 602
           ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAK                 QMVTT
Sbjct: 541 ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAK-----------------QMVTT 600

Query: 603 FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN 662
           FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN
Sbjct: 601 FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN 660

Query: 663 NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA 712
           NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA
Sbjct: 661 NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA 692

BLAST of MS014030 vs. NCBI nr
Match: KAG8502151.1 (hypothetical protein CXB51_000245 [Gossypium anomalum])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 622/711 (87.48%), Postives = 651/711 (91.56%), Query Frame = 0

Query: 2   DMDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHE 61
           +M +SSACL+G G S+H  K K  KDFYG NIV +P + SL + S A +V+ASL H +HE
Sbjct: 17  EMAASSACLLGNGLSTHRTKSKLSKDFYGRNIVFTPAISSLGRKSNAVLVKASLKHKQHE 76

Query: 62  GRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYEN 121
           GRRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+EN
Sbjct: 77  GRRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFEN 136

Query: 122 GTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIG 181
           GTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLIG
Sbjct: 137 GTIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIG 196

Query: 182 NLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDE 241
           NLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVDE
Sbjct: 197 NLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVDE 256

Query: 242 AKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGS 301
           AKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGS
Sbjct: 257 AKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGS 316

Query: 302 EFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLL 361
           EFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQLL
Sbjct: 317 EFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLL 376

Query: 362 TEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKK 421
           TEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNKK
Sbjct: 377 TEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNKK 436

Query: 422 FDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT 481
           F+TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT
Sbjct: 437 FETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT 496

Query: 482 IMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQ 541
           +MTDGKSKSLVAYHEVGHA+CGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISKQ
Sbjct: 497 VMTDGKSKSLVAYHEVGHAVCGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISKQ 556

Query: 542 QLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQM 601
           QLFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 QM
Sbjct: 557 QLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------QM 616

Query: 602 VTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAH 661
           V TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL H
Sbjct: 617 VVTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQH 676

Query: 662 IRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           IRNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 677 IRNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 710

BLAST of MS014030 vs. NCBI nr
Match: KAA3467955.1 (ATP-dependent zinc metalloprotease YME1L1 [Gossypium australe])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 623/712 (87.50%), Postives = 651/712 (91.43%), Query Frame = 0

Query: 1   IDMDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKH 60
           + M +SSACL+G G SSH  K K  KDFYG NIV +P + SL + S A +V+ASL H +H
Sbjct: 1   MQMAASSACLLGNGLSSHRTKSKLSKDFYGRNIVFTPAVSSLGRKSNAVLVKASLKHKQH 60

Query: 61  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 120
           EGRRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+E
Sbjct: 61  EGRRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFE 120

Query: 121 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 180
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLI
Sbjct: 121 NGTIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLI 180

Query: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 240
           GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300
           EAKQDF+EVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFLEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 360
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 420
           LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNK
Sbjct: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNK 420

Query: 421 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480
           KF+TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480

Query: 481 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 540
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 541 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 600
           QQLFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------Q 600

Query: 601 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 660
           MV TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL 
Sbjct: 601 MVVTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQ 660

Query: 661 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           HIRNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 661 HIRNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 695

BLAST of MS014030 vs. NCBI nr
Match: TYI41518.1 (hypothetical protein ES332_A01G032500v1 [Gossypium tomentosum] >TYI41519.1 hypothetical protein ES332_A01G032500v1 [Gossypium tomentosum])

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 622/710 (87.61%), Postives = 650/710 (91.55%), Query Frame = 0

Query: 3   MDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEG 62
           M +SSACL+G G S+H  K K  K+FYG NIV +P + SL + S A +V+ASL H +HEG
Sbjct: 1   MAASSACLLGNGLSTHRTKSKLSKEFYGTNIVFTPAISSLGRKSNAVLVKASLKHKQHEG 60

Query: 63  RRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENG 122
           RRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+ENG
Sbjct: 61  RRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENG 120

Query: 123 TIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGN 182
           TIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLIGN
Sbjct: 121 TIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 180

Query: 183 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEA 242
           LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVDEA
Sbjct: 181 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVDEA 240

Query: 243 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 302
           KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE
Sbjct: 241 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 300

Query: 303 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLT 362
           FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQLLT
Sbjct: 301 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLT 360

Query: 363 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKF 422
           EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNKKF
Sbjct: 361 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNKKF 420

Query: 423 DTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTI 482
           +TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT+
Sbjct: 421 ETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTV 480

Query: 483 MTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQ 542
           MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISKQQ
Sbjct: 481 MTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISKQQ 540

Query: 543 LFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMV 602
           LFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 QMV
Sbjct: 541 LFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------QMV 600

Query: 603 TTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHI 662
            TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL HI
Sbjct: 601 VTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQHI 660

Query: 663 RNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           RNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 661 RNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 693

BLAST of MS014030 vs. NCBI nr
Match: KHG26365.1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic -like protein [Gossypium arboreum])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 622/712 (87.36%), Postives = 651/712 (91.43%), Query Frame = 0

Query: 1   IDMDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKH 60
           + M +SSACL+G G S+H  K K  K+FYG NIV +P + SL + S A +V+ASL H +H
Sbjct: 7   MQMAASSACLLGNGLSTHRTKSKLSKEFYGRNIVFTPAISSLGRKSNAVLVKASLKHKQH 66

Query: 61  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 120
           EGRRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+E
Sbjct: 67  EGRRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFE 126

Query: 121 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 180
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLI
Sbjct: 127 NGTIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLI 186

Query: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 240
           GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 187 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVD 246

Query: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300
           EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 247 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 306

Query: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 360
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 307 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 366

Query: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 420
           LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNK
Sbjct: 367 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNK 426

Query: 421 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480
           KF+TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 427 KFETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 486

Query: 481 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 540
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 487 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISK 546

Query: 541 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 600
           QQLFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 Q
Sbjct: 547 QQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------Q 606

Query: 601 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 660
           MV TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL 
Sbjct: 607 MVVTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQ 666

Query: 661 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           HIRNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 667 HIRNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 701

BLAST of MS014030 vs. ExPASy Swiss-Prot
Match: Q655S1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FTSH2 PE=3 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 584/680 (85.88%), Postives = 618/680 (90.88%), Query Frame = 0

Query: 33  VPSPTLLSLSKVSRAAIVRASLGHGKHEGRRGFLKL-VGNVGFGVPALLGSGRANAEEQG 92
           +P  TL S     R   V ASL H  ++ RR FLKL +GN+G G+P LLG+ RA AEEQG
Sbjct: 14  LPFSTLPSSGVAQRPVSVTASLEHKTNDARRKFLKLALGNLGVGLPTLLGAKRALAEEQG 73

Query: 93  VSSSRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQELL 152
           VSSSRMSYSRFLEYLDKDRV +VDL+ENGTIAIVEAISPELGNR+QRVRVQLPGLSQELL
Sbjct: 74  VSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELL 133

Query: 153 QKLREKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFP 212
           QKLREKNIDFAAH+ QE+SGS LFNLIGNLAFPLILIGGLFLLSRR+ GG+GGP GPGFP
Sbjct: 134 QKLREKNIDFAAHSNQEDSGSLLFNLIGNLAFPLILIGGLFLLSRRAQGGLGGPNGPGFP 193

Query: 213 LGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLI 272
           LG GQS+AKFQMEPNTGV FDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLL+
Sbjct: 194 LGFGQSRAKFQMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLV 253

Query: 273 GPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVD 332
           GPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVD
Sbjct: 254 GPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVD 313

Query: 333 EIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPG 392
           EIDAVGRQRG GIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPG
Sbjct: 314 EIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPG 373

Query: 393 RFDRQVTVDVPDIRGRTDILKVHASNKKFDTDVSLDVIAMRTPGFSGADLANLLNEAAIL 452
           RFDRQV+VDVPD+RGRT+ILKVH SNKKFDTDVSL+VIAMRTPGFSGADLANLLNEAAIL
Sbjct: 374 RFDRQVSVDVPDVRGRTEILKVHGSNKKFDTDVSLEVIAMRTPGFSGADLANLLNEAAIL 433

Query: 453 AGRRGKTAISSKEIDDSIDRIVAGMEGTIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQ 512
           AGRRG+TAISSKEIDDSIDRIVAGMEGT+MTDGKSKSLVAYHEVGHAICGTLTPGHD VQ
Sbjct: 434 AGRRGRTAISSKEIDDSIDRIVAGMEGTVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQ 493

Query: 513 KVTLVPRGQARGLTWFIPADDPTLISKQQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGD 572
           KVTL+PRGQARGLTWFIP DDPTLIS+QQLFARIVGGLGGRAAEE+IFGEPEVTTGA GD
Sbjct: 494 KVTLIPRGQARGLTWFIPMDDPTLISRQQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGD 553

Query: 573 LQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMSEIGPWSLMDPSAQSADVIMRMMARN 632
           LQQITGLAK                 QMV TFGMS+IGPWSLMD  AQS DVIMRMMARN
Sbjct: 554 LQQITGLAK-----------------QMVVTFGMSDIGPWSLMDSGAQSGDVIMRMMARN 613

Query: 633 SMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREAIDKIVEILLEKETMSGDEFRAILSE 692
           SMSEKLAEDID A+KR+SDEAYEIAL+ IR+NREA+DKIVE+LLEKET+SGDEFRAILSE
Sbjct: 614 SMSEKLAEDIDTAVKRLSDEAYEIALSQIRSNREAMDKIVEVLLEKETLSGDEFRAILSE 673

Query: 693 FVEIPVENRVPPPAPAPVSA 712
           F EIPVENRVPP  PA + A
Sbjct: 674 FTEIPVENRVPPATPAALPA 676

BLAST of MS014030 vs. ExPASy Swiss-Prot
Match: Q8W585 (ATP-dependent zinc metalloprotease FTSH 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH8 PE=1 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 594/717 (82.85%), Postives = 628/717 (87.59%), Query Frame = 0

Query: 3   MDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEG 62
           M +SSACL+G G S +  K +F K             L L + S+  +V+ASL   KHEG
Sbjct: 1   MAASSACLLGNGLSVYTTKQRFQK-------------LGLDRTSKVTVVKASLDEKKHEG 60

Query: 63  RRGFLK-LVGNVGFGVPALLGSGRANAEE--QGVSSSRMSYSRFLEYLDKDRVTRVDLYE 122
           RRGF K L+GN   GV  LL SG ANA+E  QGVSSSRMSYSRFLEYLDK RV +VDLYE
Sbjct: 61  RRGFFKLLLGNAAAGV-GLLASGNANADEQGQGVSSSRMSYSRFLEYLDKGRVEKVDLYE 120

Query: 123 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 182
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQKLR KNIDFAAHNAQE+ GS + NLI
Sbjct: 121 NGTIAIVEAVSPELGNRIQRVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSPILNLI 180

Query: 183 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 242
           GNLAFP+ILIGGLFLLSRRSSGGMGGPGGPGFPL IGQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPVILIGGLFLLSRRSSGGMGGPGGPGFPLQIGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 243 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 302
           EAKQDFMEVVEFLKKPERFTAVGARIPKGVLL+GPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 303 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 362
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 363 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 422
           LTEMDGFEGNTG+IV+AATNRADILDSALLRPGRFDRQV+VDVPD++GRTDILKVH+ NK
Sbjct: 361 LTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHSGNK 420

Query: 423 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 482
           KF++ VSL+VIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFESGVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480

Query: 483 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 542
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 543 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 602
           QQLFARIVGGLGGRAAEEVIFGE EVTTGAV DLQQITGLAK                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEVIFGESEVTTGAVSDLQQITGLAK-----------------Q 600

Query: 603 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 662
           MVTTFGMSEIGPWSLMD S QS DVIMRMMARNSMSEKLA DID A+K +SD+AYEIAL+
Sbjct: 601 MVTTFGMSEIGPWSLMDSSEQS-DVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALS 660

Query: 663 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRV------PPPAPAPV 710
            IRNNREA+DKIVEILLEKETMSGDEFRAILSEF EIP ENRV        P PA V
Sbjct: 661 QIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIPPENRVASSTSTSTPTPASV 685

BLAST of MS014030 vs. ExPASy Swiss-Prot
Match: O80860 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH2 PE=1 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 586/718 (81.62%), Postives = 632/718 (88.02%), Query Frame = 0

Query: 3   MDSSSACLIGRGSS--HCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASL-GHGKH 62
           M +SSACL+G G S     K +  K F G       +  S+ + S+  +V+ASL G  K 
Sbjct: 1   MAASSACLVGNGLSVNTTTKQRLSKHFSG----RQTSFSSVIRTSKVNVVKASLDGKKKQ 60

Query: 63  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 122
           EGRR FLK L+GN G G   L+ SG+ANA+EQGVSSSRMSYSRFLEYLDKDRV +VDLYE
Sbjct: 61  EGRRDFLKILLGNAGVG---LVASGKANADEQGVSSSRMSYSRFLEYLDKDRVNKVDLYE 120

Query: 123 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 182
           NGTIAIVEA+SPELGNR++RVRVQLPGLSQELLQKLR KNIDFAAHNAQE+ GS LFNLI
Sbjct: 121 NGTIAIVEAVSPELGNRVERVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSVLFNLI 180

Query: 183 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 242
           GNLAFP +LIGGLFLLSRRS GGMGGPGGPG PL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPALLIGGLFLLSRRSGGGMGGPGGPGNPLQFGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 243 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 302
           EAKQDFMEVVEFLKKPERFTAVGA+IPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFMEVVEFLKKPERFTAVGAKIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 303 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 362
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 363 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 422
           LTEMDGFEGNTG+IV+AATNRADILDSALLRPGRFDRQV+VDVPD++GRTDILKVHA NK
Sbjct: 361 LTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHAGNK 420

Query: 423 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 482
           KFD DVSL++IAMRTPGFSGADLANLLNEAAILAGRR +T+ISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFDNDVSLEIIAMRTPGFSGADLANLLNEAAILAGRRARTSISSKEIDDSIDRIVAGMEG 480

Query: 483 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 542
           T+MTDGKSKSLVAYHEVGHA+CGTLTPGHDAVQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAVCGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 543 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 602
           QQLFARIVGGLGGRAAEE+IFG+ EVTTGAVGDLQQITGLA+                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEIIFGDSEVTTGAVGDLQQITGLAR-----------------Q 600

Query: 603 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 662
           MVTTFGMS+IGPWSLMD SAQS DVIMRMMARNSMSEKLAEDID A+K++SD AYEIAL+
Sbjct: 601 MVTTFGMSDIGPWSLMDSSAQS-DVIMRMMARNSMSEKLAEDIDSAVKKLSDSAYEIALS 660

Query: 663 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVP-----PPAPAPVSA 712
           HI+NNREA+DK+VE+LLEKET+ GDEFRAILSEF EIP ENRVP      PA AP  A
Sbjct: 661 HIKNNREAMDKLVEVLLEKETIGGDEFRAILSEFTEIPPENRVPSSTTTTPASAPTPA 693

BLAST of MS014030 vs. ExPASy Swiss-Prot
Match: Q1PDW5 (ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH6 PE=2 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.4e-250
Identity = 450/668 (67.37%), Postives = 534/668 (79.94%), Query Frame = 0

Query: 36  PTLLSLSKVSRAAIVRASLGHGKHEGRRGFLKLVGNVGFGVPALLG---SGRANAEEQG- 95
           P  LS S  +    ++  + H K   +R  L L   +GF   + LG   +  A AE +  
Sbjct: 30  PASLSKSSHTHKPSLKTQILHHKFT-KRNLLSLTTALGF--TSALGTVLAHPAKAEPEAP 89

Query: 96  --VSSSRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQE 155
              +S+RMSYSRFL++L ++ V +VDL ENGT+AIVE  +P +G ++QRVRV LPGL  +
Sbjct: 90  IEATSNRMSYSRFLQHLKENEVKKVDLIENGTVAIVEISNPVVG-KIQRVRVNLPGLPVD 149

Query: 156 LLQKLREKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGMGGPGGPG 215
           L+++++EKN+DFAAH      G+ L N +GNL FPLIL+  L L    +S     P GP 
Sbjct: 150 LVREMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFPLILLVSLLL----TSSSRRNPAGPN 209

Query: 216 FPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVL 275
            P G+G+SKAKFQMEPNTG+ F+DVAGVDEAKQDF E+VEFLK PE+F+A+GA+IPKGVL
Sbjct: 210 LPFGLGRSKAKFQMEPNTGITFEDVAGVDEAKQDFEEIVEFLKTPEKFSALGAKIPKGVL 269

Query: 276 LIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVF 335
           L GPPGTGKTLLAKAIAGEAGVPFFS+SGSEF+EMFVGVGASR RDLF KAK N+PCIVF
Sbjct: 270 LTGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFIEMFVGVGASRARDLFNKAKANSPCIVF 329

Query: 336 VDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLR 395
           +DEIDAVGR RG GIGGGNDEREQTLNQ+LTEMDGF GNTG+IVIAATNR +ILDSALLR
Sbjct: 330 IDEIDAVGRMRGTGIGGGNDEREQTLNQILTEMDGFAGNTGVIVIAATNRPEILDSALLR 389

Query: 396 PGRFDRQVTVDVPDIRGRTDILKVHASNKKFDTDVSLDVIAMRTPGFSGADLANLLNEAA 455
           PGRFDRQV+V +PDIRGR +ILKVH+ +KK D DVSL VIAMRTPGFSGADLANL+NEAA
Sbjct: 390 PGRFDRQVSVGLPDIRGREEILKVHSRSKKLDKDVSLSVIAMRTPGFSGADLANLMNEAA 449

Query: 456 ILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMTDGKSKSLVAYHEVGHAICGTLTPGHDA 515
           ILAGRRGK  I+  EIDDSIDRIVAGMEGT M DGKSK++VAYHEVGHAIC TLT GHD 
Sbjct: 450 ILAGRRGKDKITLTEIDDSIDRIVAGMEGTKMIDGKSKAIVAYHEVGHAICATLTEGHDP 509

Query: 516 VQKVTLVPRGQARGLTWFIPADDPTLISKQQLFARIVGGLGGRAAEEVIFGEPEVTTGAV 575
           VQKVTLVPRGQARGLTWF+P +DPTL+SKQQLFARIVGGLGGRAAE+VIFGEPE+TTGA 
Sbjct: 510 VQKVTLVPRGQARGLTWFLPGEDPTLVSKQQLFARIVGGLGGRAAEDVIFGEPEITTGAA 569

Query: 576 GDLQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMSEIGPWSLMDPSAQSADVIMRMMA 635
           GDLQQ+T +A+                 QMVT FGMSEIGPW+L DP+ +  DV++RM+A
Sbjct: 570 GDLQQVTEIAR-----------------QMVTMFGMSEIGPWALTDPAVKQNDVVLRMLA 629

Query: 636 RNSMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREAIDKIVEILLEKETMSGDEFRAIL 695
           RNSMSEKLAEDID  +K+I  +AYE+A  H+RNNREAIDK+V++LLEKET++GDEFRAIL
Sbjct: 630 RNSMSEKLAEDIDSCVKKIIGDAYEVAKKHVRNNREAIDKLVDVLLEKETLTGDEFRAIL 672

Query: 696 SEFVEIPV 698
           SE+ + P+
Sbjct: 690 SEYTDQPL 672

BLAST of MS014030 vs. ExPASy Swiss-Prot
Match: Q67WJ2 (ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FTSH6 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 2.2e-240
Identity = 428/643 (66.56%), Postives = 512/643 (79.63%), Query Frame = 0

Query: 62  RRGFLKLVG-NVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENG 121
           RR  L+  G  +G G+ A   +       + V+S+RMSYSRFLEYLD   V +VD +ENG
Sbjct: 53  RRRLLQSAGLGLGLGLTAARPARAEATAPEEVTSNRMSYSRFLEYLDAGAVKKVDFFENG 112

Query: 122 TIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGN 181
           T+A+ E       +R+ RV+VQLPGL  EL++KLR+K +DFAAH  +  +G  L +L+ N
Sbjct: 113 TVAVAEVDDAAALSRVHRVKVQLPGLPAELVRKLRDKGVDFAAHPVEPSAGVMLLDLLVN 172

Query: 182 LAFPLILIGGLFLLSRRSSGGMGGP-GGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDE 241
             FPL+ +  L       S  M  P GGP  P G+G+SKAKFQMEP TGV FDDVAGVDE
Sbjct: 173 FGFPLLFVASLLW----RSPTMNNPGGGPSLPFGLGKSKAKFQMEPKTGVTFDDVAGVDE 232

Query: 242 AKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGS 301
           AKQDF E+V+FLK PE+FTAVGAR PKGVLL+GPPGTGKTLLAKAIAGEAGVPFFS+SGS
Sbjct: 233 AKQDFQEIVQFLKFPEKFTAVGARTPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSLSGS 292

Query: 302 EFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLL 361
           EF+EMFVGVGASRVRDLF +AK +APC+VF+DEIDAVGRQRGAGIGGGNDEREQTLNQLL
Sbjct: 293 EFIEMFVGVGASRVRDLFDRAKASAPCLVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLL 352

Query: 362 TEMDGF-EGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 421
           TEMDGF  G+ G++VIAATNR +ILD+ALLRPGRFDR+V+V +PD+RGR +IL VH +NK
Sbjct: 353 TEMDGFGGGDGGVVVIAATNRPEILDAALLRPGRFDRRVSVGLPDVRGREEILLVHGANK 412

Query: 422 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 481
           + D  VSL V+AMRTPGFSGADLANL+NEAAILAGRRGK  I+  EIDDSIDRIVAG+EG
Sbjct: 413 RLDPGVSLAVVAMRTPGFSGADLANLMNEAAILAGRRGKDRITVSEIDDSIDRIVAGLEG 472

Query: 482 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPA-DDPTLIS 541
           T MTDGKSK LVAYHE+GHA+C TLT GHD VQKVTL+PRGQARGLTWF+P  +DP L+S
Sbjct: 473 TSMTDGKSKMLVAYHEIGHAVCATLTAGHDEVQKVTLIPRGQARGLTWFLPGEEDPALVS 532

Query: 542 KQQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCL 601
           +QQ+FA IVGGLGGRAAEEV+FGEPEVTTGA GDLQQ+T +A+                 
Sbjct: 533 RQQIFAGIVGGLGGRAAEEVVFGEPEVTTGAAGDLQQVTRVAR----------------- 592

Query: 602 QMVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIAL 661
           +MVT FGMSEIGPW+L +P+AQ  DV++RM+AR+SMSE+LA DID A++ I DEAYE+A 
Sbjct: 593 RMVTAFGMSEIGPWALAEPAAQGGDVVLRMLARSSMSERLAADIDAAVRTIVDEAYEVAK 652

Query: 662 AHIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENR 701
           AH+R NR AID++V++L+EKET+ GDEFRAILSE V+I  E R
Sbjct: 653 AHVRRNRAAIDQLVDVLMEKETLGGDEFRAILSEHVDIGKERR 674

BLAST of MS014030 vs. ExPASy TrEMBL
Match: A0A6J1D970 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111018069 PE=3 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 692/709 (97.60%), Postives = 692/709 (97.60%), Query Frame = 0

Query: 3   MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR 62
           MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR
Sbjct: 1   MDSSSACLIGRGSSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEGR 60

Query: 63  RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI 122
           RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI
Sbjct: 61  RGFLKLVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENGTI 120

Query: 123 AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA 182
           AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA
Sbjct: 121 AIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGNLA 180

Query: 183 FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ 242
           FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ
Sbjct: 181 FPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQ 240

Query: 243 DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV 302
           DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV
Sbjct: 241 DFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFV 300

Query: 303 EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM 362
           EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM
Sbjct: 301 EMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEM 360

Query: 363 DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT 422
           DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT
Sbjct: 361 DGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKFDT 420

Query: 423 DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT 482
           DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT
Sbjct: 421 DVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMT 480

Query: 483 DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF 542
           DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF
Sbjct: 481 DGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLF 540

Query: 543 ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMVTT 602
           ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAK                 QMVTT
Sbjct: 541 ARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAK-----------------QMVTT 600

Query: 603 FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN 662
           FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN
Sbjct: 601 FGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRN 660

Query: 663 NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA 712
           NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA
Sbjct: 661 NREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVSA 692

BLAST of MS014030 vs. ExPASy TrEMBL
Match: A0A5B6VFH6 (ATP-dependent zinc metalloprotease YME1L1 OS=Gossypium australe OX=47621 GN=ftsH PE=3 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 623/712 (87.50%), Postives = 651/712 (91.43%), Query Frame = 0

Query: 1   IDMDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKH 60
           + M +SSACL+G G SSH  K K  KDFYG NIV +P + SL + S A +V+ASL H +H
Sbjct: 1   MQMAASSACLLGNGLSSHRTKSKLSKDFYGRNIVFTPAVSSLGRKSNAVLVKASLKHKQH 60

Query: 61  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 120
           EGRRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+E
Sbjct: 61  EGRRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFE 120

Query: 121 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 180
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLI
Sbjct: 121 NGTIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLI 180

Query: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 240
           GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300
           EAKQDF+EVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFLEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 360
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 420
           LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNK
Sbjct: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNK 420

Query: 421 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480
           KF+TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480

Query: 481 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 540
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 541 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 600
           QQLFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------Q 600

Query: 601 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 660
           MV TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL 
Sbjct: 601 MVVTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQ 660

Query: 661 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           HIRNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 661 HIRNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 695

BLAST of MS014030 vs. ExPASy TrEMBL
Match: A0A5D2RNC2 (AAA domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A01G032500v1 PE=3 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 622/710 (87.61%), Postives = 650/710 (91.55%), Query Frame = 0

Query: 3   MDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEG 62
           M +SSACL+G G S+H  K K  K+FYG NIV +P + SL + S A +V+ASL H +HEG
Sbjct: 1   MAASSACLLGNGLSTHRTKSKLSKEFYGTNIVFTPAISSLGRKSNAVLVKASLKHKQHEG 60

Query: 63  RRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENG 122
           RRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+ENG
Sbjct: 61  RRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENG 120

Query: 123 TIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGN 182
           TIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLIGN
Sbjct: 121 TIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 180

Query: 183 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEA 242
           LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVDEA
Sbjct: 181 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVDEA 240

Query: 243 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 302
           KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE
Sbjct: 241 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 300

Query: 303 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLT 362
           FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQLLT
Sbjct: 301 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLT 360

Query: 363 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKF 422
           EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNKKF
Sbjct: 361 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNKKF 420

Query: 423 DTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTI 482
           +TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT+
Sbjct: 421 ETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTV 480

Query: 483 MTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQ 542
           MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISKQQ
Sbjct: 481 MTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISKQQ 540

Query: 543 LFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMV 602
           LFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 QMV
Sbjct: 541 LFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------QMV 600

Query: 603 TTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHI 662
            TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL HI
Sbjct: 601 VTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQHI 660

Query: 663 RNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           RNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 661 RNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 693

BLAST of MS014030 vs. ExPASy TrEMBL
Match: A0A0B0PMY7 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like protein OS=Gossypium arboreum OX=29729 GN=F383_05722 PE=3 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 622/712 (87.36%), Postives = 651/712 (91.43%), Query Frame = 0

Query: 1   IDMDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKH 60
           + M +SSACL+G G S+H  K K  K+FYG NIV +P + SL + S A +V+ASL H +H
Sbjct: 7   MQMAASSACLLGNGLSTHRTKSKLSKEFYGRNIVFTPAISSLGRKSNAVLVKASLKHKQH 66

Query: 61  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 120
           EGRRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+E
Sbjct: 67  EGRRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFE 126

Query: 121 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 180
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLI
Sbjct: 127 NGTIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLI 186

Query: 181 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 240
           GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 187 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVD 246

Query: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300
           EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 247 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 306

Query: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 360
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 307 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 366

Query: 361 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 420
           LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNK
Sbjct: 367 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNK 426

Query: 421 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480
           KF+TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 427 KFETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 486

Query: 481 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 540
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 487 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISK 546

Query: 541 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 600
           QQLFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 Q
Sbjct: 547 QQLFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------Q 606

Query: 601 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 660
           MV TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL 
Sbjct: 607 MVVTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQ 666

Query: 661 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           HIRNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 667 HIRNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 701

BLAST of MS014030 vs. ExPASy TrEMBL
Match: A0A6P4PF80 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Gossypium arboreum OX=29729 GN=LOC108483365 PE=3 SV=1)

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 622/710 (87.61%), Postives = 650/710 (91.55%), Query Frame = 0

Query: 3   MDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEG 62
           M +SSACL+G G S+H  K K  K+FYG NIV +P + SL + S A +V+ASL H +HEG
Sbjct: 1   MAASSACLLGNGLSTHRTKSKLSKEFYGRNIVFTPAISSLGRKSNAVLVKASLKHKQHEG 60

Query: 63  RRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYENG 122
           RRGFLK L+G  G GVPALLG+G+A A+EQGVSSSRMSYSRFLEYLDKDRV +VDL+ENG
Sbjct: 61  RRGFLKSLLGTAGIGVPALLGNGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENG 120

Query: 123 TIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLIGN 182
           TIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK REKNIDFAAHNAQE+SGS LFNLIGN
Sbjct: 121 TIAIVEAVSPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 180

Query: 183 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVDEA 242
           LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPL  GQSKAKFQMEPNTGV FDDVAGVDEA
Sbjct: 181 LAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLAFGQSKAKFQMEPNTGVTFDDVAGVDEA 240

Query: 243 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 302
           KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE
Sbjct: 241 KQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSE 300

Query: 303 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQLLT 362
           FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQLLT
Sbjct: 301 FVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLT 360

Query: 363 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNKKF 422
           EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRT+ILKVH SNKKF
Sbjct: 361 EMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTEILKVHGSNKKF 420

Query: 423 DTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTI 482
           +TDVS DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGT+
Sbjct: 421 ETDVSFDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTV 480

Query: 483 MTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQ 542
           MTDGKSKSLVAYHEVGHAICGTLTPGHD VQKVTL+PRGQARGLTWFIP+DDPTLISKQQ
Sbjct: 481 MTDGKSKSLVAYHEVGHAICGTLTPGHDPVQKVTLIPRGQARGLTWFIPSDDPTLISKQQ 540

Query: 543 LFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMV 602
           LFARIVGGLGGRAAEE+IFGEPEVTTGA GDLQQITGLAK                 QMV
Sbjct: 541 LFARIVGGLGGRAAEEIIFGEPEVTTGAAGDLQQITGLAK-----------------QMV 600

Query: 603 TTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHI 662
            TFGMSEIGPWSLMD SAQSADVIMRMMARNSMSEKLAEDID A+KRISD AYEIAL HI
Sbjct: 601 VTFGMSEIGPWSLMDSSAQSADVIMRMMARNSMSEKLAEDIDTAVKRISDSAYEIALQHI 660

Query: 663 RNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVPPPAPAPVS 711
           RNNREAIDKIVE+LLEKETMSGDEFRAILSEFVEIP EN+VPP  P PVS
Sbjct: 661 RNNREAIDKIVEVLLEKETMSGDEFRAILSEFVEIPAENQVPPSVPTPVS 693

BLAST of MS014030 vs. TAIR 10
Match: AT1G06430.1 (FTSH protease 8 )

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 594/717 (82.85%), Postives = 628/717 (87.59%), Query Frame = 0

Query: 3   MDSSSACLIGRG-SSHCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASLGHGKHEG 62
           M +SSACL+G G S +  K +F K             L L + S+  +V+ASL   KHEG
Sbjct: 1   MAASSACLLGNGLSVYTTKQRFQK-------------LGLDRTSKVTVVKASLDEKKHEG 60

Query: 63  RRGFLK-LVGNVGFGVPALLGSGRANAEE--QGVSSSRMSYSRFLEYLDKDRVTRVDLYE 122
           RRGF K L+GN   GV  LL SG ANA+E  QGVSSSRMSYSRFLEYLDK RV +VDLYE
Sbjct: 61  RRGFFKLLLGNAAAGV-GLLASGNANADEQGQGVSSSRMSYSRFLEYLDKGRVEKVDLYE 120

Query: 123 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 182
           NGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQKLR KNIDFAAHNAQE+ GS + NLI
Sbjct: 121 NGTIAIVEAVSPELGNRIQRVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSPILNLI 180

Query: 183 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 242
           GNLAFP+ILIGGLFLLSRRSSGGMGGPGGPGFPL IGQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPVILIGGLFLLSRRSSGGMGGPGGPGFPLQIGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 243 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 302
           EAKQDFMEVVEFLKKPERFTAVGARIPKGVLL+GPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 303 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 362
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 363 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 422
           LTEMDGFEGNTG+IV+AATNRADILDSALLRPGRFDRQV+VDVPD++GRTDILKVH+ NK
Sbjct: 361 LTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHSGNK 420

Query: 423 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 482
           KF++ VSL+VIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFESGVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 480

Query: 483 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 542
           T+MTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 543 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 602
           QQLFARIVGGLGGRAAEEVIFGE EVTTGAV DLQQITGLAK                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEVIFGESEVTTGAVSDLQQITGLAK-----------------Q 600

Query: 603 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 662
           MVTTFGMSEIGPWSLMD S QS DVIMRMMARNSMSEKLA DID A+K +SD+AYEIAL+
Sbjct: 601 MVTTFGMSEIGPWSLMDSSEQS-DVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALS 660

Query: 663 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRV------PPPAPAPV 710
            IRNNREA+DKIVEILLEKETMSGDEFRAILSEF EIP ENRV        P PA V
Sbjct: 661 QIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIPPENRVASSTSTSTPTPASV 685

BLAST of MS014030 vs. TAIR 10
Match: AT2G30950.1 (FtsH extracellular protease family )

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 586/718 (81.62%), Postives = 632/718 (88.02%), Query Frame = 0

Query: 3   MDSSSACLIGRGSS--HCAKLKFCKDFYGVNIVPSPTLLSLSKVSRAAIVRASL-GHGKH 62
           M +SSACL+G G S     K +  K F G       +  S+ + S+  +V+ASL G  K 
Sbjct: 1   MAASSACLVGNGLSVNTTTKQRLSKHFSG----RQTSFSSVIRTSKVNVVKASLDGKKKQ 60

Query: 63  EGRRGFLK-LVGNVGFGVPALLGSGRANAEEQGVSSSRMSYSRFLEYLDKDRVTRVDLYE 122
           EGRR FLK L+GN G G   L+ SG+ANA+EQGVSSSRMSYSRFLEYLDKDRV +VDLYE
Sbjct: 61  EGRRDFLKILLGNAGVG---LVASGKANADEQGVSSSRMSYSRFLEYLDKDRVNKVDLYE 120

Query: 123 NGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKLREKNIDFAAHNAQEESGSALFNLI 182
           NGTIAIVEA+SPELGNR++RVRVQLPGLSQELLQKLR KNIDFAAHNAQE+ GS LFNLI
Sbjct: 121 NGTIAIVEAVSPELGNRVERVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSVLFNLI 180

Query: 183 GNLAFPLILIGGLFLLSRRSSGGMGGPGGPGFPLGIGQSKAKFQMEPNTGVNFDDVAGVD 242
           GNLAFP +LIGGLFLLSRRS GGMGGPGGPG PL  GQSKAKFQMEPNTGV FDDVAGVD
Sbjct: 181 GNLAFPALLIGGLFLLSRRSGGGMGGPGGPGNPLQFGQSKAKFQMEPNTGVTFDDVAGVD 240

Query: 243 EAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 302
           EAKQDFMEVVEFLKKPERFTAVGA+IPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG
Sbjct: 241 EAKQDFMEVVEFLKKPERFTAVGAKIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISG 300

Query: 303 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGAGIGGGNDEREQTLNQL 362
           SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRG GIGGGNDEREQTLNQL
Sbjct: 301 SEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQL 360

Query: 363 LTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVTVDVPDIRGRTDILKVHASNK 422
           LTEMDGFEGNTG+IV+AATNRADILDSALLRPGRFDRQV+VDVPD++GRTDILKVHA NK
Sbjct: 361 LTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHAGNK 420

Query: 423 KFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEG 482
           KFD DVSL++IAMRTPGFSGADLANLLNEAAILAGRR +T+ISSKEIDDSIDRIVAGMEG
Sbjct: 421 KFDNDVSLEIIAMRTPGFSGADLANLLNEAAILAGRRARTSISSKEIDDSIDRIVAGMEG 480

Query: 483 TIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISK 542
           T+MTDGKSKSLVAYHEVGHA+CGTLTPGHDAVQKVTL+PRGQARGLTWFIP+DDPTLISK
Sbjct: 481 TVMTDGKSKSLVAYHEVGHAVCGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISK 540

Query: 543 QQLFARIVGGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQ 602
           QQLFARIVGGLGGRAAEE+IFG+ EVTTGAVGDLQQITGLA+                 Q
Sbjct: 541 QQLFARIVGGLGGRAAEEIIFGDSEVTTGAVGDLQQITGLAR-----------------Q 600

Query: 603 MVTTFGMSEIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALA 662
           MVTTFGMS+IGPWSLMD SAQS DVIMRMMARNSMSEKLAEDID A+K++SD AYEIAL+
Sbjct: 601 MVTTFGMSDIGPWSLMDSSAQS-DVIMRMMARNSMSEKLAEDIDSAVKKLSDSAYEIALS 660

Query: 663 HIRNNREAIDKIVEILLEKETMSGDEFRAILSEFVEIPVENRVP-----PPAPAPVSA 712
           HI+NNREA+DK+VE+LLEKET+ GDEFRAILSEF EIP ENRVP      PA AP  A
Sbjct: 661 HIKNNREAMDKLVEVLLEKETIGGDEFRAILSEFTEIPPENRVPSSTTTTPASAPTPA 693

BLAST of MS014030 vs. TAIR 10
Match: AT5G15250.1 (FTSH protease 6 )

HSP 1 Score: 867.1 bits (2239), Expect = 1.0e-251
Identity = 450/668 (67.37%), Postives = 534/668 (79.94%), Query Frame = 0

Query: 36  PTLLSLSKVSRAAIVRASLGHGKHEGRRGFLKLVGNVGFGVPALLG---SGRANAEEQG- 95
           P  LS S  +    ++  + H K   +R  L L   +GF   + LG   +  A AE +  
Sbjct: 30  PASLSKSSHTHKPSLKTQILHHKFT-KRNLLSLTTALGF--TSALGTVLAHPAKAEPEAP 89

Query: 96  --VSSSRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQE 155
              +S+RMSYSRFL++L ++ V +VDL ENGT+AIVE  +P +G ++QRVRV LPGL  +
Sbjct: 90  IEATSNRMSYSRFLQHLKENEVKKVDLIENGTVAIVEISNPVVG-KIQRVRVNLPGLPVD 149

Query: 156 LLQKLREKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGMGGPGGPG 215
           L+++++EKN+DFAAH      G+ L N +GNL FPLIL+  L L    +S     P GP 
Sbjct: 150 LVREMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFPLILLVSLLL----TSSSRRNPAGPN 209

Query: 216 FPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVL 275
            P G+G+SKAKFQMEPNTG+ F+DVAGVDEAKQDF E+VEFLK PE+F+A+GA+IPKGVL
Sbjct: 210 LPFGLGRSKAKFQMEPNTGITFEDVAGVDEAKQDFEEIVEFLKTPEKFSALGAKIPKGVL 269

Query: 276 LIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVF 335
           L GPPGTGKTLLAKAIAGEAGVPFFS+SGSEF+EMFVGVGASR RDLF KAK N+PCIVF
Sbjct: 270 LTGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFIEMFVGVGASRARDLFNKAKANSPCIVF 329

Query: 336 VDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLR 395
           +DEIDAVGR RG GIGGGNDEREQTLNQ+LTEMDGF GNTG+IVIAATNR +ILDSALLR
Sbjct: 330 IDEIDAVGRMRGTGIGGGNDEREQTLNQILTEMDGFAGNTGVIVIAATNRPEILDSALLR 389

Query: 396 PGRFDRQVTVDVPDIRGRTDILKVHASNKKFDTDVSLDVIAMRTPGFSGADLANLLNEAA 455
           PGRFDRQV+V +PDIRGR +ILKVH+ +KK D DVSL VIAMRTPGFSGADLANL+NEAA
Sbjct: 390 PGRFDRQVSVGLPDIRGREEILKVHSRSKKLDKDVSLSVIAMRTPGFSGADLANLMNEAA 449

Query: 456 ILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMTDGKSKSLVAYHEVGHAICGTLTPGHDA 515
           ILAGRRGK  I+  EIDDSIDRIVAGMEGT M DGKSK++VAYHEVGHAIC TLT GHD 
Sbjct: 450 ILAGRRGKDKITLTEIDDSIDRIVAGMEGTKMIDGKSKAIVAYHEVGHAICATLTEGHDP 509

Query: 516 VQKVTLVPRGQARGLTWFIPADDPTLISKQQLFARIVGGLGGRAAEEVIFGEPEVTTGAV 575
           VQKVTLVPRGQARGLTWF+P +DPTL+SKQQLFARIVGGLGGRAAE+VIFGEPE+TTGA 
Sbjct: 510 VQKVTLVPRGQARGLTWFLPGEDPTLVSKQQLFARIVGGLGGRAAEDVIFGEPEITTGAA 569

Query: 576 GDLQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMSEIGPWSLMDPSAQSADVIMRMMA 635
           GDLQQ+T +A+                 QMVT FGMSEIGPW+L DP+ +  DV++RM+A
Sbjct: 570 GDLQQVTEIAR-----------------QMVTMFGMSEIGPWALTDPAVKQNDVVLRMLA 629

Query: 636 RNSMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREAIDKIVEILLEKETMSGDEFRAIL 695
           RNSMSEKLAEDID  +K+I  +AYE+A  H+RNNREAIDK+V++LLEKET++GDEFRAIL
Sbjct: 630 RNSMSEKLAEDIDSCVKKIIGDAYEVAKKHVRNNREAIDKLVDVLLEKETLTGDEFRAIL 672

Query: 696 SEFVEIPV 698
           SE+ + P+
Sbjct: 690 SEYTDQPL 672

BLAST of MS014030 vs. TAIR 10
Match: AT5G15250.2 (FTSH protease 6 )

HSP 1 Score: 854.0 bits (2205), Expect = 8.7e-248
Identity = 450/691 (65.12%), Postives = 534/691 (77.28%), Query Frame = 0

Query: 36  PTLLSLSKVSRAAIVRASLGHGKHEGRRGFLKLVGNVGFGVPALLG---SGRANAEEQG- 95
           P  LS S  +    ++  + H K   +R  L L   +GF   + LG   +  A AE +  
Sbjct: 28  PASLSKSSHTHKPSLKTQILHHKFT-KRNLLSLTTALGF--TSALGTVLAHPAKAEPEAP 87

Query: 96  --VSSSRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQE 155
              +S+RMSYSRFL++L ++ V +VDL ENGT+AIVE  +P +G ++QRVRV LPGL  +
Sbjct: 88  IEATSNRMSYSRFLQHLKENEVKKVDLIENGTVAIVEISNPVVG-KIQRVRVNLPGLPVD 147

Query: 156 LLQKLREKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGMGGPGGPG 215
           L+++++EKN+DFAAH      G+ L N +GNL FPLIL+  L L    +S     P GP 
Sbjct: 148 LVREMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFPLILLVSLLL----TSSSRRNPAGPN 207

Query: 216 FPLGIGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVL 275
            P G+G+SKAKFQMEPNTG+ F+DVAGVDEAKQDF E+VEFLK PE+F+A+GA+IPKGVL
Sbjct: 208 LPFGLGRSKAKFQMEPNTGITFEDVAGVDEAKQDFEEIVEFLKTPEKFSALGAKIPKGVL 267

Query: 276 LIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVF 335
           L GPPGTGKTLLAKAIAGEAGVPFFS+SGSEF+EMFVGVGASR RDLF KAK N+PCIVF
Sbjct: 268 LTGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFIEMFVGVGASRARDLFNKAKANSPCIVF 327

Query: 336 VDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLR 395
           +DEIDAVGR RG GIGGGNDEREQTLNQ+LTEMDGF GNTG+IVIAATNR +ILDSALLR
Sbjct: 328 IDEIDAVGRMRGTGIGGGNDEREQTLNQILTEMDGFAGNTGVIVIAATNRPEILDSALLR 387

Query: 396 PGRFDR-----------------------QVTVDVPDIRGRTDILKVHASNKKFDTDVSL 455
           PGRFDR                       QV+V +PDIRGR +ILKVH+ +KK D DVSL
Sbjct: 388 PGRFDRQVCWLILKPNKSNRFGIMSTCFKQVSVGLPDIRGREEILKVHSRSKKLDKDVSL 447

Query: 456 DVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISSKEIDDSIDRIVAGMEGTIMTDGKS 515
            VIAMRTPGFSGADLANL+NEAAILAGRRGK  I+  EIDDSIDRIVAGMEGT M DGKS
Sbjct: 448 SVIAMRTPGFSGADLANLMNEAAILAGRRGKDKITLTEIDDSIDRIVAGMEGTKMIDGKS 507

Query: 516 KSLVAYHEVGHAICGTLTPGHDAVQKVTLVPRGQARGLTWFIPADDPTLISKQQLFARIV 575
           K++VAYHEVGHAIC TLT GHD VQKVTLVPRGQARGLTWF+P +DPTL+SKQQLFARIV
Sbjct: 508 KAIVAYHEVGHAICATLTEGHDPVQKVTLVPRGQARGLTWFLPGEDPTLVSKQQLFARIV 567

Query: 576 GGLGGRAAEEVIFGEPEVTTGAVGDLQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMS 635
           GGLGGRAAE+VIFGEPE+TTGA GDLQQ+T +A+                 QMVT FGMS
Sbjct: 568 GGLGGRAAEDVIFGEPEITTGAAGDLQQVTEIAR-----------------QMVTMFGMS 627

Query: 636 EIGPWSLMDPSAQSADVIMRMMARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREA 695
           EIGPW+L DP+ +  DV++RM+ARNSMSEKLAEDID  +K+I  +AYE+A  H+RNNREA
Sbjct: 628 EIGPWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKKHVRNNREA 687

Query: 696 IDKIVEILLEKETMSGDEFRAILSEFVEIPV 698
           IDK+V++LLEKET++GDEFRAILSE+ + P+
Sbjct: 688 IDKLVDVLLEKETLTGDEFRAILSEYTDQPL 693

BLAST of MS014030 vs. TAIR 10
Match: AT1G50250.1 (FTSH protease 1 )

HSP 1 Score: 567.4 bits (1461), Expect = 1.6e-161
Identity = 310/600 (51.67%), Postives = 411/600 (68.50%), Query Frame = 0

Query: 95  SRMSYSRFLEYLDKDRVTRVDLYENGTIAIVEAISPELGNRLQRVRVQLPGLSQELLQKL 154
           S+  YS FL  + K +V RV   ++G++  + A+     NR  R  V +P    +L+  L
Sbjct: 132 SQWRYSEFLNAVKKGKVERVRFSKDGSVVQLTAVD----NR--RASVIVPN-DPDLIDIL 191

Query: 155 REKNIDFAAHNAQEESGSALFNLIGNLAFPLILIGGLFLLSRRSSGGM-GGPGGPGFPLG 214
               +D +     E SG+ LF +IGNL FPL+  GGLFLL RR+ GG  GGPGG G P+ 
Sbjct: 192 AMNGVDISVSEG-ESSGNDLFTVIGNLIFPLLAFGGLFLLFRRAQGGPGGGPGGLGGPMD 251

Query: 215 IGQSKAKFQMEPNTGVNFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLIGP 274
            G+SK+KFQ  P TGV+F DVAG D+AK +  EVV+FLK P+++TA+GA+IPKG LL+GP
Sbjct: 252 FGRSKSKFQEVPETGVSFADVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGP 311

Query: 275 PGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEI 334
           PGTGKTLLA+A+AGEAGVPFFS + SEFVE+FVGVGASRVRDLF+KAK  APCIVF+DEI
Sbjct: 312 PGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEI 371

Query: 335 DAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPGRF 394
           DAVGRQRGAG+GGGNDEREQT+NQLLTEMDGF GN+G+IV+AATNR D+LDSALLRPGRF
Sbjct: 372 DAVGRQRGAGMGGGNDEREQTINQLLTEMDGFSGNSGVIVLAATNRPDVLDSALLRPGRF 431

Query: 395 DRQVTVDVPDIRGRTDILKVHASNKKFDTDVSLDVIAMRTPGFSGADLANLLNEAAILAG 454
           DRQVTVD PD+ GR  IL+VH+  K    DV  D +A RTPGF+GADL NL+NEAAILA 
Sbjct: 432 DRQVTVDRPDVAGRVKILQVHSRGKALGKDVDFDKVARRTPGFTGADLQNLMNEAAILAA 491

Query: 455 RRGKTAISSKEIDDSIDRIVAGME-GTIMTDGKSKSLVAYHEVGHAICGTLTPGHDAVQK 514
           RR    IS  EI D+++RI+AG E    +   + K LVAYHE GHA+ G L P +D V K
Sbjct: 492 RRELKEISKDEISDALERIIAGPEKKNAVVSEEKKRLVAYHEAGHALVGALMPEYDPVAK 551

Query: 515 VTLVPRGQARGLTWFIPAD---DPTLISKQQLFARIVGGLGGRAAEEVIFGEPEVTTGAV 574
           ++++PRGQA GLT+F P++   +  L S+  L  ++   LGGR AEEVIFG+  VTTGA 
Sbjct: 552 ISIIPRGQAGGLTFFAPSEERLESGLYSRSYLENQMAVALGGRVAEEVIFGDENVTTGAS 611

Query: 575 GDLQQITGLAKQVCRTSPFCLLVISTCLQMVTTFGMS-EIGPWSLMDPSAQSADVIMRMM 634
            D  Q++ +A+                 QM+  FG S +IG  ++  P   +  +  +M 
Sbjct: 612 NDFMQVSRVAR-----------------QMIERFGFSKKIGQVAVGGPGG-NPFMGQQMS 671

Query: 635 ARNSMSEKLAEDIDMAIKRISDEAYEIALAHIRNNREAIDKIVEILLEKETMSGDEFRAI 689
           ++   S   A+ +D  ++ + ++AY+ A   I  + + + K+ ++L+EKET+ G+EF ++
Sbjct: 672 SQKDYSMATADIVDAEVRELVEKAYKRATEIITTHIDILHKLAQLLIEKETVDGEEFMSL 705

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149706.10.0e+0097.60ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like [Momordica charant... [more]
KAG8502151.10.0e+0087.48hypothetical protein CXB51_000245 [Gossypium anomalum][more]
KAA3467955.10.0e+0087.50ATP-dependent zinc metalloprotease YME1L1 [Gossypium australe][more]
TYI41518.10.0e+0087.61hypothetical protein ES332_A01G032500v1 [Gossypium tomentosum] >TYI41519.1 hypot... [more]
KHG26365.10.0e+0087.36ATP-dependent zinc metalloprotease FTSH 2, chloroplastic -like protein [Gossypiu... [more]
Match NameE-valueIdentityDescription
Q655S10.0e+0085.88ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Oryza sativa subsp. ... [more]
Q8W5850.0e+0082.85ATP-dependent zinc metalloprotease FTSH 8, chloroplastic OS=Arabidopsis thaliana... [more]
O808600.0e+0081.62ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Arabidopsis thaliana... [more]
Q1PDW51.4e-25067.37ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Arabidopsis thaliana... [more]
Q67WJ22.2e-24066.56ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Oryza sativa subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1D9700.0e+0097.60ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like OS=Momordica chara... [more]
A0A5B6VFH60.0e+0087.50ATP-dependent zinc metalloprotease YME1L1 OS=Gossypium australe OX=47621 GN=ftsH... [more]
A0A5D2RNC20.0e+0087.61AAA domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A01G0325... [more]
A0A0B0PMY70.0e+0087.36ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like protein OS=Gossypi... [more]
A0A6P4PF800.0e+0087.61ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Gossypium arboreum O... [more]
Match NameE-valueIdentityDescription
AT1G06430.10.0e+0082.85FTSH protease 8 [more]
AT2G30950.10.0e+0081.62FtsH extracellular protease family [more]
AT5G15250.11.0e-25167.37FTSH protease 6 [more]
AT5G15250.28.7e-24865.12FTSH protease 6 [more]
AT1G50250.11.6e-16151.67FTSH protease 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 264..403
e-value: 3.6E-24
score: 96.3
IPR000642Peptidase M41PFAMPF01434Peptidase_M41coord: 597..688
e-value: 1.1E-19
score: 71.0
coord: 482..582
e-value: 1.8E-39
score: 135.5
IPR037219Peptidase M41-likeGENE3D1.20.58.760Peptidase M41coord: 481..694
e-value: 5.6E-56
score: 191.6
IPR037219Peptidase M41-likeSUPERFAMILY140990FtsH protease domain-likecoord: 483..697
IPR005936Peptidase, FtsHTIGRFAMTIGR01241TIGR01241coord: 176..689
e-value: 4.0E-204
score: 676.9
IPR005936Peptidase, FtsHHAMAPMF_01458FtsHcoord: 25..705
score: 34.106892
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 268..399
e-value: 2.4E-45
score: 154.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 219..401
e-value: 1.5E-70
score: 238.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 227..471
NoneNo IPR availableGENE3D1.10.8.60coord: 402..480
e-value: 9.3E-22
score: 78.8
NoneNo IPR availableGENE3D3.30.720.210coord: 91..171
e-value: 3.6E-7
score: 31.9
NoneNo IPR availablePANTHERPTHR23076:SF100ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 2, CHLOROPLASTICcoord: 7..701
NoneNo IPR availablePANTHERPTHR23076METALLOPROTEASE M41 FTSHcoord: 7..701
NoneNo IPR availableCDDcd00009AAAcoord: 243..400
e-value: 9.11839E-28
score: 107.617
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 423..466
e-value: 4.7E-12
score: 45.5
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 371..389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014030.1MS014030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0004222 metalloendopeptidase activity