MS014022 (gene) Bitter gourd (TR) v1

BLAST of MS014022 vs. NCBI nr
Match: XP_022149687.1 (transmembrane E3 ubiquitin-protein ligase 1-like [Momordica charantia])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 567/569 (99.65%), Postives = 567/569 (99.65%), Query Frame = 0

Query: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKD 60
           MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLV QPVDGLRPLRERARSWGDEWLFVRKD
Sbjct: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVSQPVDGLRPLRERARSWGDEWLFVRKD 60

Query: 61  KSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120
           KSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ
Sbjct: 61  KSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120

Query: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180
           GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL
Sbjct: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180

Query: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL 240
           GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL
Sbjct: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL 240

Query: 241 MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ 300
           MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ
Sbjct: 241 MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ 300

Query: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360
           SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA
Sbjct: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360

Query: 361 IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ 420
           IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ
Sbjct: 361 IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ 420

Query: 421 IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV 480
           IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV
Sbjct: 421 IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV 480

Query: 481 AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC 540
           AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC
Sbjct: 481 AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC 540

Query: 541 DHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           DHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 DHFFHSGCLQRWMDIKMECPTCRRLLPPA 569

BLAST of MS014022 vs. NCBI nr
Match: XP_038901005.1 (transmembrane E3 ubiquitin-protein ligase FLY2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 538/569 (94.55%), Postives = 550/569 (96.66%), Query Frame = 0

Query: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKD 60
           MVSR  FG SE QRR G FSRTVFALW   FLV QPVDGLRPLRERARSWGDEWLFVRKD
Sbjct: 1   MVSREGFGCSEFQRRFGYFSRTVFALWVVQFLVSQPVDGLRPLRERARSWGDEWLFVRKD 60

Query: 61  KSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120
           KSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ
Sbjct: 61  KSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120

Query: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180
           GVVIFHDVI NEHDV GVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL
Sbjct: 121 GVVIFHDVIGNEHDVGGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180

Query: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL 240
           GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQIS+FSSAQNVNGE +HYHIEGL
Sbjct: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISYFSSAQNVNGEHDHYHIEGL 240

Query: 241 MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ 300
           MESPAVDDDGDCFSPL LNATSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNTQ
Sbjct: 241 MESPAVDDDGDCFSPLALNATSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNTQ 300

Query: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360
           SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA
Sbjct: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360

Query: 361 IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ 420
           IWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIPQ
Sbjct: 361 IWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIPQ 420

Query: 421 IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV 480
           IVTNV+RDSRKPLHI+YILGIT+TRLAIP+YVFGCPHNFMRIQ DKTWCICLGVFT LQV
Sbjct: 421 IVTNVIRDSRKPLHIHYILGITITRLAIPLYVFGCPHNFMRIQTDKTWCICLGVFTFLQV 480

Query: 481 AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC 540
           AILLLQHY GSRWFIPRQILPEKYSYYRR+DQNGNHATDCVICMTTID++RPNDCMITPC
Sbjct: 481 AILLLQHYFGSRWFIPRQILPEKYSYYRRIDQNGNHATDCVICMTTIDVSRPNDCMITPC 540

Query: 541 DHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           DHFFHS CLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 DHFFHSVCLQRWMDIKMECPTCRRLLPPA 569

BLAST of MS014022 vs. NCBI nr
Match: XP_008448263.1 (PREDICTED: transmembrane E3 ubiquitin-protein ligase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 535/570 (93.86%), Postives = 548/570 (96.14%), Query Frame = 0

Query: 1   MVSRGRFGFSECQ-RRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R   G SE Q    G FSRTV ALW  H LV QPVDGLRPLRERARSWGDEWLFVRK
Sbjct: 1   MVGREGLGCSEFQISGFGLFSRTVIALWVIHCLVSQPVDGLRPLRERARSWGDEWLFVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DKSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEH+V GVQIKVEGVYIWPFRQLRMVANSGKGGE+KQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHEVGGVQIKVEGVYIWPFRQLRMVANSGKGGEFKQEVDSILSNPYHL 180

Query: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEG 240
           LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGE +HYHIEG
Sbjct: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEHDHYHIEG 240

Query: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNT 300
           LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNT
Sbjct: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNT 300

Query: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360
           QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL
Sbjct: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360

Query: 361 AIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIP 420
           AIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIP
Sbjct: 361 AIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIP 420

Query: 421 QIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQ 480
           QIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCICLGVFT LQ
Sbjct: 421 QIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCICLGVFTFLQ 480

Query: 481 VAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITP 540
           VAILLLQHY GSRWFIPRQ+LPEKYSYYRR+DQNGNHATDCVICMTTID++RPNDCMITP
Sbjct: 481 VAILLLQHYFGSRWFIPRQVLPEKYSYYRRIDQNGNHATDCVICMTTIDVSRPNDCMITP 540

Query: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           CDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570

BLAST of MS014022 vs. NCBI nr
Match: XP_038901004.1 (transmembrane E3 ubiquitin-protein ligase FLY2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 550/578 (95.16%), Query Frame = 0

Query: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKD 60
           MVSR  FG SE QRR G FSRTVFALW   FLV QPVDGLRPLRERARSWGDEWLFVRKD
Sbjct: 1   MVSREGFGCSEFQRRFGYFSRTVFALWVVQFLVSQPVDGLRPLRERARSWGDEWLFVRKD 60

Query: 61  KSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120
           KSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ
Sbjct: 61  KSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120

Query: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL- 180
           GVVIFHDVI NEHDV GVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 
Sbjct: 121 GVVIFHDVIGNEHDVGGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLA 180

Query: 181 --------LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGE 240
                   LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQIS+FSSAQNVNGE
Sbjct: 181 RLPPSRINLGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISYFSSAQNVNGE 240

Query: 241 REHYHIEGLMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLI 300
            +HYHIEGLMESPAVDDDGDCFSPL LNATSVNIEIYYNKAVNYTLMVTF+SFLQVLLLI
Sbjct: 241 HDHYHIEGLMESPAVDDDGDCFSPLALNATSVNIEIYYNKAVNYTLMVTFISFLQVLLLI 300

Query: 301 RQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFS 360
           RQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFS
Sbjct: 301 RQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFS 360

Query: 361 IFEMRYLLAIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILL 420
           IFEMRYLLAIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILL
Sbjct: 361 IFEMRYLLAIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILL 420

Query: 421 LMYSFWIPQIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCIC 480
           LMYSFWIPQIVTNV+RDSRKPLHI+YILGIT+TRLAIP+YVFGCPHNFMRIQ DKTWCIC
Sbjct: 421 LMYSFWIPQIVTNVIRDSRKPLHIHYILGITITRLAIPLYVFGCPHNFMRIQTDKTWCIC 480

Query: 481 LGVFTSLQVAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITR 540
           LGVFT LQVAILLLQHY GSRWFIPRQILPEKYSYYRR+DQNGNHATDCVICMTTID++R
Sbjct: 481 LGVFTFLQVAILLLQHYFGSRWFIPRQILPEKYSYYRRIDQNGNHATDCVICMTTIDVSR 540

Query: 541 PNDCMITPCDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           PNDCMITPCDHFFHS CLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 PNDCMITPCDHFFHSVCLQRWMDIKMECPTCRRLLPPA 578

BLAST of MS014022 vs. NCBI nr
Match: XP_022985942.1 (transmembrane E3 ubiquitin-protein ligase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 533/570 (93.51%), Postives = 545/570 (95.61%), Query Frame = 0

Query: 1   MVSRGRFGFSECQR-RLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R  FG SE +R   G FSRTVFALW  H LV QPVDGLRPLRERARSWGDEWL VRK
Sbjct: 1   MVGREGFGCSEFRRGGFGYFSRTVFALWVVHILVSQPVDGLRPLRERARSWGDEWLLVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DK+DLGPFSEWNITGTYRG WSF DSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKNDLGPFSEWNITGTYRGSWSFQDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEHDV GVQIK+EGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHDVGGVQIKIEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180

Query: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEG 240
           LGVFSSQVFQESPQ+ MWRRSHSP YEMEKHCNIEIAAQISH SSAQNVNGE +HYHIEG
Sbjct: 181 LGVFSSQVFQESPQENMWRRSHSPTYEMEKHCNIEIAAQISHSSSAQNVNGEHDHYHIEG 240

Query: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNT 300
           LMESPAVDDDGDCFSPLVLN TSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNT
Sbjct: 241 LMESPAVDDDGDCFSPLVLNGTSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNT 300

Query: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360
           QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL
Sbjct: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360

Query: 361 AIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIP 420
           AIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIP
Sbjct: 361 AIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIP 420

Query: 421 QIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQ 480
           QIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCICLGVFT LQ
Sbjct: 421 QIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCICLGVFTFLQ 480

Query: 481 VAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITP 540
           VAILLLQHY GSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDI+RPNDCMITP
Sbjct: 481 VAILLLQHYFGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDISRPNDCMITP 540

Query: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           CDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570

BLAST of MS014022 vs. ExPASy Swiss-Prot
Match: Q500V2 (Transmembrane E3 ubiquitin-protein ligase FLY2 OS=Arabidopsis thaliana OX=3702 GN=FLY2 PE=2 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.1e-257
Identity = 425/557 (76.30%), Postives = 481/557 (86.36%), Query Frame = 0

Query: 16  LGNFSRTVFALWAFH--FLVPQPVDGLRPLRERARSWGDEWLFVRKDKSDLGPFSEWNIT 75
           LGNF    F  ++    F V Q  +GLRP+RE ARSWGDEWLF +K+K   GPFS WNIT
Sbjct: 4   LGNFGVWGFGFFSLSIWFAVLQQANGLRPIRETARSWGDEWLFGKKEKGGAGPFSAWNIT 63

Query: 76  GTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQGVVIFHDVIDNEH 135
           GTYRG W FLD+ N+SS+FPDFR  +GNS+IEL+++PTKI+GVHYVQG V+FHDV DNEH
Sbjct: 64  GTYRGTWKFLDTVNSSSKFPDFRKESGNSVIELVTSPTKITGVHYVQGAVVFHDVFDNEH 123

Query: 136 DVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLLGVFSSQVFQESPQ 195
           +V G QIKVEGVYIWPFRQLR+VANSGK  +   E D +LSNPYHLLG+FSSQVFQESP+
Sbjct: 124 NVGGAQIKVEGVYIWPFRQLRLVANSGKKSDSGLEDDYLLSNPYHLLGIFSSQVFQESPR 183

Query: 196 DKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGLMESPAVDDDGDCF 255
           D++ ++  SPIYEMEKHCNIEIAAQIS  +S++N NG+++ Y IEGLMESPAVDD+ DCF
Sbjct: 184 DRILKQKTSPIYEMEKHCNIEIAAQISQSTSSEN-NGDKDRYQIEGLMESPAVDDEVDCF 243

Query: 256 SPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSILMIGQ 315
           SPL LNATS+N+E+YYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSI+MIGQ
Sbjct: 244 SPLSLNATSINVEVYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSIVMIGQ 303

Query: 316 QAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKASRPMNSGEG 375
           QAIMD+YLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL+IWKA+RP  SGEG
Sbjct: 304 QAIMDSYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLSIWKATRPSTSGEG 363

Query: 376 WETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQIVTNVVRDSRKPL 435
           WETMRRELS LYSRFYGILLGGI +MYE H ++RPILLLMYSFWIPQIV NVVRDSRKPL
Sbjct: 364 WETMRRELSFLYSRFYGILLGGILLMYEFHNYMRPILLLMYSFWIPQIVANVVRDSRKPL 423

Query: 436 HIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYLGSRW 495
           H YYILG+TVTRLAIP+YVFGCP NFMR++P K WC+ L  F   Q  +LLLQHY GSR 
Sbjct: 424 HPYYILGMTVTRLAIPLYVFGCPKNFMRVEPSKAWCVSLCAFMGFQAGVLLLQHYFGSRC 483

Query: 496 FIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDIT-RPNDCMITPCDHFFHSGCLQRW 555
           F+PR++LPEKYSYYRRLD N N + DCVICMTTID+  R NDCM+TPC+H FHSGCLQRW
Sbjct: 484 FVPRKLLPEKYSYYRRLDHNVNRSRDCVICMTTIDLRHRINDCMVTPCEHIFHSGCLQRW 543

Query: 556 MDIKMECPTCRRLLPPA 570
           MDIKMECPTCRR LPPA
Sbjct: 544 MDIKMECPTCRRPLPPA 559

BLAST of MS014022 vs. ExPASy Swiss-Prot
Match: Q5PP23 (Transmembrane E3 ubiquitin-protein ligase FLY1 OS=Arabidopsis thaliana OX=3702 GN=FLY1 PE=1 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 1.4e-253
Identity = 413/555 (74.41%), Postives = 480/555 (86.49%), Query Frame = 0

Query: 16  LGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKDKSDLGPFSEWNITGT 75
           LG F   +    +    + Q   GLRP+RE+ RSW DEWLF RK ++++GPFS WNITGT
Sbjct: 9   LGFFEWQIILWLSIWLAISQQALGLRPIREKPRSWSDEWLFGRKQEAEVGPFSAWNITGT 68

Query: 76  YRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQGVVIFHDVIDNEHDV 135
           YRG W FL+S N+SS+F DF+  NGNS++EL++ PTKI+GVHYVQGVV+FHDV DNE +V
Sbjct: 69  YRGTWKFLNSLNSSSKFQDFQKENGNSVVELVAVPTKITGVHYVQGVVVFHDVFDNEQNV 128

Query: 136 AGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLLGVFSSQVFQESPQDK 195
            G QI +EGVYIWPFRQLR+VANSGK  +  QE +++LSNPYHLLG+FSSQVFQESP+D+
Sbjct: 129 GGAQINLEGVYIWPFRQLRLVANSGKESDSGQEDNNLLSNPYHLLGIFSSQVFQESPRDR 188

Query: 196 MWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGLMESPAVDDDGDCFSP 255
           + +R  SP+ EMEKHCNIEIAAQ+S  +S++N NG++ +YH+EGLMESP V DDGDCFSP
Sbjct: 189 LLKRKLSPVNEMEKHCNIEIAAQVSRVASSEN-NGDKNYYHMEGLMESPGVGDDGDCFSP 248

Query: 256 LVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSILMIGQQA 315
           L+LNATSVN+E+YYNKAVNYTLMVTFVSFLQVLLLIRQMEH NTQSGAAKVSI+MIGQQA
Sbjct: 249 LLLNATSVNVEVYYNKAVNYTLMVTFVSFLQVLLLIRQMEHGNTQSGAAKVSIVMIGQQA 308

Query: 316 IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKASRPMNSGEGWE 375
           IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKA+RP NSGEGWE
Sbjct: 309 IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKATRPSNSGEGWE 368

Query: 376 TMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQIVTNVVRDSRKPLHI 435
           TMRRELS LYSRFYGILLGGI IMY+ H +++PILLLMYSFWIPQIV NVVRDSRKPLH 
Sbjct: 369 TMRRELSFLYSRFYGILLGGILIMYQFHNYMQPILLLMYSFWIPQIVANVVRDSRKPLHP 428

Query: 436 YYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYLGSRWFI 495
           YYILG+T TRLAIP+YVFGCPHNFMR++P+K WCICL  F  LQ  ILLLQHY GSR F+
Sbjct: 429 YYILGMTATRLAIPLYVFGCPHNFMRVEPNKVWCICLCTFMGLQAVILLLQHYFGSRCFV 488

Query: 496 PRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITR-PNDCMITPCDHFFHSGCLQRWMD 555
           PRQ+LPEKY+Y+RR +++ +  TDCVICMT ID+ +  +DCM+TPC+HFFHSGCLQRWMD
Sbjct: 489 PRQMLPEKYNYHRRFNRDVSRTTDCVICMTAIDLRQHTSDCMVTPCEHFFHSGCLQRWMD 548

Query: 556 IKMECPTCRRLLPPA 570
           IKMECPTCRR LPPA
Sbjct: 549 IKMECPTCRRSLPPA 562

BLAST of MS014022 vs. ExPASy Swiss-Prot
Match: P36096 (Transmembrane E3 ubiquitin-protein ligase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TUL1 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 9.8e-37
Identity = 112/398 (28.14%), Postives = 177/398 (44.47%), Query Frame = 0

Query: 237 IEGLMESPAVDDDGDCFSPLVL-NATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQME 296
           + GL+ SP      DC   L L N      E+       + L    +   Q+ LL+ QM 
Sbjct: 366 VSGLLYSP------DCGVRLGLHNVKGTRYELKIMSIRKHLLFGIALFAAQIYLLLTQMH 425

Query: 297 HSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEM 356
           H+NT S   K+S        ++D  L  L+  A  +V  L+     +AF  F++ SIFE+
Sbjct: 426 HTNTPSMVNKISFYCFSMINLVDGSLATLYFVAASVVPELYLPLVISAFSCFILASIFEI 485

Query: 357 RYLLAIWKAS-----------RPMNSGEGWETMRRELSV---------LYSRFYGILLGG 416
           RYL++I+ +               N+G   E   R   +         LY RF+ +L+  
Sbjct: 486 RYLISIYASQVNEQNVGIINLLRGNTGTYDENRPRPAFIPDEGSIGGSLYGRFFFMLIIF 545

Query: 417 IFIMYELHRFLRP--------ILLLMYSFWIPQIVTNVVR------------------DS 476
            F++     + R         ++ ++ S+WIPQI  N V+                   +
Sbjct: 546 TFLILSSTSWPRQLRMVFEYILIFILNSYWIPQIFRNAVKGIPSRRERARSSIGGNRSQN 605

Query: 477 RKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYL 536
           + PL   +++G T+ R    +YVF    N  R   D  + + L ++   Q++IL  Q  L
Sbjct: 606 KMPLLWSFVIGTTIIRSLPVVYVFTYSSNVFRHHKDVHFVVFLSLWLLFQISILYSQDVL 665

Query: 537 GSRWFIPRQILPEKYSYYRRLDQ---------NGNHATDCVICMTTIDI----------T 569
           GSRWF+P+  +P+ YSY++ L              H  DC ICM+ + I           
Sbjct: 666 GSRWFLPKHTIPDGYSYFKPLSNEYISEHGGGTAEHTVDCAICMSDVPIYIEEIPETHKV 725

BLAST of MS014022 vs. ExPASy Swiss-Prot
Match: O43085 (DSC E3 ubiquitin ligase complex subunit 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dsc1 PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 2.2e-28
Identity = 110/424 (25.94%), Postives = 179/424 (42.22%), Query Frame = 0

Query: 231 EREHYHIEGL--MESPAVDDDGDCFSP------LVLNATSVNIEIYYNKAVNYTLMVTFV 290
           E E YH +G+   + P V   G  +SP         N       +  N  V ++ +  F+
Sbjct: 272 ENEFYHPQGVSTQKMPEVFVSGLVYSPDCNVAFTFSNTKGPRNFVLENHLVRFSSLYIFI 331

Query: 291 SFLQVLLLIRQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATA 350
              Q+ +L+RQM   N+ S   ++S L I  QA +DAY+ +  L+   ++E  +  F + 
Sbjct: 332 VLSQIFVLLRQM-RINSPSHVQRLSFLTIAMQAGLDAYIAIFFLSTNAVIEKGYLPFVSV 391

Query: 351 AFFKFVVFSIFEMRYLLAIWK---------ASRPMNSGEG-------------------- 410
           AF   V   +F MRYL  I +         A RP+ +                       
Sbjct: 392 AFLSLVPSVMFTMRYLALILRVQNSNMPPPAPRPVTNNSSNNNTNQSNASNENSPNAPSA 451

Query: 411 ----------------------WETMRRELSVLYSRFYGILL----GGIF-----IMYEL 470
                                  E  +R+ S +  RFY I+L      ++     ++Y  
Sbjct: 452 ANDNTETTTVNPPQEDDQPMTQHERDQRDWSAVCLRFYFIILVVCIASLYSAFWPVIYRF 511

Query: 471 HRFLRPILLLMYSFWIPQIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRI 530
           + F+  ++   YSFWIPQI+ NV + + +     YILG +V RL +P+ +F      +  
Sbjct: 512 Y-FISALIFTSYSFWIPQIIQNVKQGTSRSFTWTYILGASVLRLYLPLAIFIDSELILGF 571

Query: 531 QPDKTWCICLGVFTSLQVAILLLQHYLGSRWFIPRQILPEK--YSYYRRLDQNG-----N 568
            P   + + L ++   QV +LL+Q  LG R+F+P++       Y Y+  + Q+       
Sbjct: 572 PPKYFFALGLVLWMLFQVLVLLVQDTLGPRFFLPKKFFLSSPVYDYHPVIQQDDLEAFMR 631

BLAST of MS014022 vs. ExPASy Swiss-Prot
Match: Q9ZT49 (Probable E3 ubiquitin-protein ligase ATL45 OS=Arabidopsis thaliana OX=3702 GN=ATL45 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.0e-06
Identity = 21/57 (36.84%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 513 NGNHATDCVICMTTIDITRPNDCMITP-CDHFFHSGCLQRWMDIKMECPTCRRLLPP 569
           +G+ +T+C IC+T  + +   +  I P C H FH  C+ +W+  +  CP+CRR+L P
Sbjct: 106 DGDSSTECAICIT--EFSEGEEIRILPLCSHAFHVACIDKWLTSRSSCPSCRRILVP 160

BLAST of MS014022 vs. ExPASy TrEMBL
Match: A0A6J1D8N7 (transmembrane E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111018054 PE=4 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 567/569 (99.65%), Postives = 567/569 (99.65%), Query Frame = 0

Query: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKD 60
           MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLV QPVDGLRPLRERARSWGDEWLFVRKD
Sbjct: 1   MVSRGRFGFSECQRRLGNFSRTVFALWAFHFLVSQPVDGLRPLRERARSWGDEWLFVRKD 60

Query: 61  KSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120
           KSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ
Sbjct: 61  KSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQ 120

Query: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180
           GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL
Sbjct: 121 GVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLL 180

Query: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL 240
           GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL
Sbjct: 181 GVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGL 240

Query: 241 MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ 300
           MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ
Sbjct: 241 MESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQ 300

Query: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360
           SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA
Sbjct: 301 SGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLA 360

Query: 361 IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ 420
           IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ
Sbjct: 361 IWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQ 420

Query: 421 IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV 480
           IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV
Sbjct: 421 IVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQV 480

Query: 481 AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC 540
           AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC
Sbjct: 481 AILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITPC 540

Query: 541 DHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           DHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 DHFFHSGCLQRWMDIKMECPTCRRLLPPA 569

BLAST of MS014022 vs. ExPASy TrEMBL
Match: A0A1S3BJA5 (transmembrane E3 ubiquitin-protein ligase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490504 PE=4 SV=1)

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 535/570 (93.86%), Postives = 548/570 (96.14%), Query Frame = 0

Query: 1   MVSRGRFGFSECQ-RRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R   G SE Q    G FSRTV ALW  H LV QPVDGLRPLRERARSWGDEWLFVRK
Sbjct: 1   MVGREGLGCSEFQISGFGLFSRTVIALWVIHCLVSQPVDGLRPLRERARSWGDEWLFVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DKSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEH+V GVQIKVEGVYIWPFRQLRMVANSGKGGE+KQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHEVGGVQIKVEGVYIWPFRQLRMVANSGKGGEFKQEVDSILSNPYHL 180

Query: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEG 240
           LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGE +HYHIEG
Sbjct: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEHDHYHIEG 240

Query: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNT 300
           LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNT
Sbjct: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNT 300

Query: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360
           QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL
Sbjct: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360

Query: 361 AIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIP 420
           AIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIP
Sbjct: 361 AIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIP 420

Query: 421 QIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQ 480
           QIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCICLGVFT LQ
Sbjct: 421 QIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCICLGVFTFLQ 480

Query: 481 VAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITP 540
           VAILLLQHY GSRWFIPRQ+LPEKYSYYRR+DQNGNHATDCVICMTTID++RPNDCMITP
Sbjct: 481 VAILLLQHYFGSRWFIPRQVLPEKYSYYRRIDQNGNHATDCVICMTTIDVSRPNDCMITP 540

Query: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           CDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570

BLAST of MS014022 vs. ExPASy TrEMBL
Match: A0A6J1J691 (transmembrane E3 ubiquitin-protein ligase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483834 PE=4 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 533/570 (93.51%), Postives = 545/570 (95.61%), Query Frame = 0

Query: 1   MVSRGRFGFSECQR-RLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R  FG SE +R   G FSRTVFALW  H LV QPVDGLRPLRERARSWGDEWL VRK
Sbjct: 1   MVGREGFGCSEFRRGGFGYFSRTVFALWVVHILVSQPVDGLRPLRERARSWGDEWLLVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DK+DLGPFSEWNITGTYRG WSF DSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKNDLGPFSEWNITGTYRGSWSFQDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEHDV GVQIK+EGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHDVGGVQIKIEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180

Query: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEG 240
           LGVFSSQVFQESPQ+ MWRRSHSP YEMEKHCNIEIAAQISH SSAQNVNGE +HYHIEG
Sbjct: 181 LGVFSSQVFQESPQENMWRRSHSPTYEMEKHCNIEIAAQISHSSSAQNVNGEHDHYHIEG 240

Query: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNT 300
           LMESPAVDDDGDCFSPLVLN TSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNT
Sbjct: 241 LMESPAVDDDGDCFSPLVLNGTSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNT 300

Query: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360
           QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL
Sbjct: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360

Query: 361 AIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIP 420
           AIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIP
Sbjct: 361 AIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIP 420

Query: 421 QIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQ 480
           QIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCICLGVFT LQ
Sbjct: 421 QIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCICLGVFTFLQ 480

Query: 481 VAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITP 540
           VAILLLQHY GSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDI+RPNDCMITP
Sbjct: 481 VAILLLQHYFGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDISRPNDCMITP 540

Query: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           CDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570

BLAST of MS014022 vs. ExPASy TrEMBL
Match: A0A1S3BIP6 (transmembrane E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490504 PE=4 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 535/579 (92.40%), Postives = 548/579 (94.65%), Query Frame = 0

Query: 1   MVSRGRFGFSECQ-RRLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R   G SE Q    G FSRTV ALW  H LV QPVDGLRPLRERARSWGDEWLFVRK
Sbjct: 1   MVGREGLGCSEFQISGFGLFSRTVIALWVIHCLVSQPVDGLRPLRERARSWGDEWLFVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DKSDLGPFSEWNITGTYRG WSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKSDLGPFSEWNITGTYRGSWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEH+V GVQIKVEGVYIWPFRQLRMVANSGKGGE+KQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHEVGGVQIKVEGVYIWPFRQLRMVANSGKGGEFKQEVDSILSNPYHL 180

Query: 181 ---------LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNG 240
                    LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNG
Sbjct: 181 ARLPPSRINLGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNG 240

Query: 241 EREHYHIEGLMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLL 300
           E +HYHIEGLMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTF+SFLQVLLL
Sbjct: 241 EHDHYHIEGLMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFISFLQVLLL 300

Query: 301 IRQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVF 360
           IRQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVF
Sbjct: 301 IRQMEHSNTQSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVF 360

Query: 361 SIFEMRYLLAIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPIL 420
           SIFEMRYLLAIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPIL
Sbjct: 361 SIFEMRYLLAIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPIL 420

Query: 421 LLMYSFWIPQIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCI 480
           LLMYSFWIPQIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCI
Sbjct: 421 LLMYSFWIPQIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCI 480

Query: 481 CLGVFTSLQVAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDIT 540
           CLGVFT LQVAILLLQHY GSRWFIPRQ+LPEKYSYYRR+DQNGNHATDCVICMTTID++
Sbjct: 481 CLGVFTFLQVAILLLQHYFGSRWFIPRQVLPEKYSYYRRIDQNGNHATDCVICMTTIDVS 540

Query: 541 RPNDCMITPCDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           RPNDCMITPCDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 RPNDCMITPCDHFFHSGCLQRWMDIKMECPTCRRLLPPA 579

BLAST of MS014022 vs. ExPASy TrEMBL
Match: A0A6J1FX69 (DSC E3 ubiquitin ligase complex subunit 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448323 PE=4 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 529/570 (92.81%), Postives = 545/570 (95.61%), Query Frame = 0

Query: 1   MVSRGRFGFSECQR-RLGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRK 60
           MV R  FG S  +R   G FSRTVF+LW  H LV QPVDGLRPLRERARSWGDEWL VRK
Sbjct: 1   MVGREGFGCSSFRRGGFGYFSRTVFSLWVVHILVSQPVDGLRPLRERARSWGDEWLLVRK 60

Query: 61  DKSDLGPFSEWNITGTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120
           DK+DLGPFSEWNITGTYRG WSF DSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV
Sbjct: 61  DKNDLGPFSEWNITGTYRGSWSFQDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYV 120

Query: 121 QGVVIFHDVIDNEHDVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180
           QGVVIFHDVI NEHDV GVQIK+EGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL
Sbjct: 121 QGVVIFHDVIGNEHDVGGVQIKIEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHL 180

Query: 181 LGVFSSQVFQESPQDKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEG 240
           LGVFSSQVFQESPQ+ MWRRSHSP+YEMEKHCNIEIAAQISH SSAQNVNGE +HYHIEG
Sbjct: 181 LGVFSSQVFQESPQENMWRRSHSPMYEMEKHCNIEIAAQISHSSSAQNVNGEHDHYHIEG 240

Query: 241 LMESPAVDDDGDCFSPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNT 300
           LMESPAVDDDGDCFSP+VLN TSVNIEIYYNKAVNYTLMVTF+SFLQVLLLIRQMEHSNT
Sbjct: 241 LMESPAVDDDGDCFSPVVLNGTSVNIEIYYNKAVNYTLMVTFISFLQVLLLIRQMEHSNT 300

Query: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360
           QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL
Sbjct: 301 QSGAAKVSILMIGQQAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL 360

Query: 361 AIWKASRPMNSGEGWETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIP 420
           AIWKASRPMN+GEGWETMRRELSVLYSRFYGILLGGIF+MYELHRFLRPILLLMYSFWIP
Sbjct: 361 AIWKASRPMNNGEGWETMRRELSVLYSRFYGILLGGIFLMYELHRFLRPILLLMYSFWIP 420

Query: 421 QIVTNVVRDSRKPLHIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQ 480
           QIVTNV+RDSRKPLHIYYILGIT+TRLAIP+YVFGCPHNFMRIQPDKTWCICLGVFT LQ
Sbjct: 421 QIVTNVIRDSRKPLHIYYILGITITRLAIPLYVFGCPHNFMRIQPDKTWCICLGVFTFLQ 480

Query: 481 VAILLLQHYLGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITRPNDCMITP 540
           VAILLLQHY GSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTID++RPNDCMITP
Sbjct: 481 VAILLLQHYFGSRWFIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDVSRPNDCMITP 540

Query: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570
           CDHFFHSGCLQRWMDIKMECPTCRRLLPPA
Sbjct: 541 CDHFFHSGCLQRWMDIKMECPTCRRLLPPA 570

BLAST of MS014022 vs. TAIR 10
Match: AT2G20650.1 (RING/U-box superfamily protein )

HSP 1 Score: 887.9 bits (2293), Expect = 4.4e-258
Identity = 425/557 (76.30%), Postives = 481/557 (86.36%), Query Frame = 0

Query: 16  LGNFSRTVFALWAFH--FLVPQPVDGLRPLRERARSWGDEWLFVRKDKSDLGPFSEWNIT 75
           LGNF    F  ++    F V Q  +GLRP+RE ARSWGDEWLF +K+K   GPFS WNIT
Sbjct: 4   LGNFGVWGFGFFSLSIWFAVLQQANGLRPIRETARSWGDEWLFGKKEKGGAGPFSAWNIT 63

Query: 76  GTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQGVVIFHDVIDNEH 135
           GTYRG W FLD+ N+SS+FPDFR  +GNS+IEL+++PTKI+GVHYVQG V+FHDV DNEH
Sbjct: 64  GTYRGTWKFLDTVNSSSKFPDFRKESGNSVIELVTSPTKITGVHYVQGAVVFHDVFDNEH 123

Query: 136 DVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLLGVFSSQVFQESPQ 195
           +V G QIKVEGVYIWPFRQLR+VANSGK  +   E D +LSNPYHLLG+FSSQVFQESP+
Sbjct: 124 NVGGAQIKVEGVYIWPFRQLRLVANSGKKSDSGLEDDYLLSNPYHLLGIFSSQVFQESPR 183

Query: 196 DKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGLMESPAVDDDGDCF 255
           D++ ++  SPIYEMEKHCNIEIAAQIS  +S++N NG+++ Y IEGLMESPAVDD+ DCF
Sbjct: 184 DRILKQKTSPIYEMEKHCNIEIAAQISQSTSSEN-NGDKDRYQIEGLMESPAVDDEVDCF 243

Query: 256 SPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSILMIGQ 315
           SPL LNATS+N+E+YYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSI+MIGQ
Sbjct: 244 SPLSLNATSINVEVYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSIVMIGQ 303

Query: 316 QAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKASRPMNSGEG 375
           QAIMD+YLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL+IWKA+RP  SGEG
Sbjct: 304 QAIMDSYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLSIWKATRPSTSGEG 363

Query: 376 WETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQIVTNVVRDSRKPL 435
           WETMRRELS LYSRFYGILLGGI +MYE H ++RPILLLMYSFWIPQIV NVVRDSRKPL
Sbjct: 364 WETMRRELSFLYSRFYGILLGGILLMYEFHNYMRPILLLMYSFWIPQIVANVVRDSRKPL 423

Query: 436 HIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYLGSRW 495
           H YYILG+TVTRLAIP+YVFGCP NFMR++P K WC+ L  F   Q  +LLLQHY GSR 
Sbjct: 424 HPYYILGMTVTRLAIPLYVFGCPKNFMRVEPSKAWCVSLCAFMGFQAGVLLLQHYFGSRC 483

Query: 496 FIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDIT-RPNDCMITPCDHFFHSGCLQRW 555
           F+PR++LPEKYSYYRRLD N N + DCVICMTTID+  R NDCM+TPC+H FHSGCLQRW
Sbjct: 484 FVPRKLLPEKYSYYRRLDHNVNRSRDCVICMTTIDLRHRINDCMVTPCEHIFHSGCLQRW 543

Query: 556 MDIKMECPTCRRLLPPA 570
           MDIKMECPTCRR LPPA
Sbjct: 544 MDIKMECPTCRRPLPPA 559

BLAST of MS014022 vs. TAIR 10
Match: AT2G20650.2 (RING/U-box superfamily protein )

HSP 1 Score: 887.9 bits (2293), Expect = 4.4e-258
Identity = 425/557 (76.30%), Postives = 481/557 (86.36%), Query Frame = 0

Query: 16  LGNFSRTVFALWAFH--FLVPQPVDGLRPLRERARSWGDEWLFVRKDKSDLGPFSEWNIT 75
           LGNF    F  ++    F V Q  +GLRP+RE ARSWGDEWLF +K+K   GPFS WNIT
Sbjct: 4   LGNFGVWGFGFFSLSIWFAVLQQANGLRPIRETARSWGDEWLFGKKEKGGAGPFSAWNIT 63

Query: 76  GTYRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQGVVIFHDVIDNEH 135
           GTYRG W FLD+ N+SS+FPDFR  +GNS+IEL+++PTKI+GVHYVQG V+FHDV DNEH
Sbjct: 64  GTYRGTWKFLDTVNSSSKFPDFRKESGNSVIELVTSPTKITGVHYVQGAVVFHDVFDNEH 123

Query: 136 DVAGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLLGVFSSQVFQESPQ 195
           +V G QIKVEGVYIWPFRQLR+VANSGK  +   E D +LSNPYHLLG+FSSQVFQESP+
Sbjct: 124 NVGGAQIKVEGVYIWPFRQLRLVANSGKKSDSGLEDDYLLSNPYHLLGIFSSQVFQESPR 183

Query: 196 DKMWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGLMESPAVDDDGDCF 255
           D++ ++  SPIYEMEKHCNIEIAAQIS  +S++N NG+++ Y IEGLMESPAVDD+ DCF
Sbjct: 184 DRILKQKTSPIYEMEKHCNIEIAAQISQSTSSEN-NGDKDRYQIEGLMESPAVDDEVDCF 243

Query: 256 SPLVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSILMIGQ 315
           SPL LNATS+N+E+YYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSI+MIGQ
Sbjct: 244 SPLSLNATSINVEVYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSIVMIGQ 303

Query: 316 QAIMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKASRPMNSGEG 375
           QAIMD+YLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLL+IWKA+RP  SGEG
Sbjct: 304 QAIMDSYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLSIWKATRPSTSGEG 363

Query: 376 WETMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQIVTNVVRDSRKPL 435
           WETMRRELS LYSRFYGILLGGI +MYE H ++RPILLLMYSFWIPQIV NVVRDSRKPL
Sbjct: 364 WETMRRELSFLYSRFYGILLGGILLMYEFHNYMRPILLLMYSFWIPQIVANVVRDSRKPL 423

Query: 436 HIYYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYLGSRW 495
           H YYILG+TVTRLAIP+YVFGCP NFMR++P K WC+ L  F   Q  +LLLQHY GSR 
Sbjct: 424 HPYYILGMTVTRLAIPLYVFGCPKNFMRVEPSKAWCVSLCAFMGFQAGVLLLQHYFGSRC 483

Query: 496 FIPRQILPEKYSYYRRLDQNGNHATDCVICMTTIDIT-RPNDCMITPCDHFFHSGCLQRW 555
           F+PR++LPEKYSYYRRLD N N + DCVICMTTID+  R NDCM+TPC+H FHSGCLQRW
Sbjct: 484 FVPRKLLPEKYSYYRRLDHNVNRSRDCVICMTTIDLRHRINDCMVTPCEHIFHSGCLQRW 543

Query: 556 MDIKMECPTCRRLLPPA 570
           MDIKMECPTCRR LPPA
Sbjct: 544 MDIKMECPTCRRPLPPA 559

BLAST of MS014022 vs. TAIR 10
Match: AT4G28370.1 (RING/U-box superfamily protein )

HSP 1 Score: 876.7 bits (2264), Expect = 1.0e-254
Identity = 413/555 (74.41%), Postives = 480/555 (86.49%), Query Frame = 0

Query: 16  LGNFSRTVFALWAFHFLVPQPVDGLRPLRERARSWGDEWLFVRKDKSDLGPFSEWNITGT 75
           LG F   +    +    + Q   GLRP+RE+ RSW DEWLF RK ++++GPFS WNITGT
Sbjct: 9   LGFFEWQIILWLSIWLAISQQALGLRPIREKPRSWSDEWLFGRKQEAEVGPFSAWNITGT 68

Query: 76  YRGFWSFLDSTNNSSRFPDFRNSNGNSIIELISTPTKISGVHYVQGVVIFHDVIDNEHDV 135
           YRG W FL+S N+SS+F DF+  NGNS++EL++ PTKI+GVHYVQGVV+FHDV DNE +V
Sbjct: 69  YRGTWKFLNSLNSSSKFQDFQKENGNSVVELVAVPTKITGVHYVQGVVVFHDVFDNEQNV 128

Query: 136 AGVQIKVEGVYIWPFRQLRMVANSGKGGEYKQEVDSILSNPYHLLGVFSSQVFQESPQDK 195
            G QI +EGVYIWPFRQLR+VANSGK  +  QE +++LSNPYHLLG+FSSQVFQESP+D+
Sbjct: 129 GGAQINLEGVYIWPFRQLRLVANSGKESDSGQEDNNLLSNPYHLLGIFSSQVFQESPRDR 188

Query: 196 MWRRSHSPIYEMEKHCNIEIAAQISHFSSAQNVNGEREHYHIEGLMESPAVDDDGDCFSP 255
           + +R  SP+ EMEKHCNIEIAAQ+S  +S++N NG++ +YH+EGLMESP V DDGDCFSP
Sbjct: 189 LLKRKLSPVNEMEKHCNIEIAAQVSRVASSEN-NGDKNYYHMEGLMESPGVGDDGDCFSP 248

Query: 256 LVLNATSVNIEIYYNKAVNYTLMVTFVSFLQVLLLIRQMEHSNTQSGAAKVSILMIGQQA 315
           L+LNATSVN+E+YYNKAVNYTLMVTFVSFLQVLLLIRQMEH NTQSGAAKVSI+MIGQQA
Sbjct: 249 LLLNATSVNVEVYYNKAVNYTLMVTFVSFLQVLLLIRQMEHGNTQSGAAKVSIVMIGQQA 308

Query: 316 IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKASRPMNSGEGWE 375
           IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKA+RP NSGEGWE
Sbjct: 309 IMDAYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLAIWKATRPSNSGEGWE 368

Query: 376 TMRRELSVLYSRFYGILLGGIFIMYELHRFLRPILLLMYSFWIPQIVTNVVRDSRKPLHI 435
           TMRRELS LYSRFYGILLGGI IMY+ H +++PILLLMYSFWIPQIV NVVRDSRKPLH 
Sbjct: 369 TMRRELSFLYSRFYGILLGGILIMYQFHNYMQPILLLMYSFWIPQIVANVVRDSRKPLHP 428

Query: 436 YYILGITVTRLAIPMYVFGCPHNFMRIQPDKTWCICLGVFTSLQVAILLLQHYLGSRWFI 495
           YYILG+T TRLAIP+YVFGCPHNFMR++P+K WCICL  F  LQ  ILLLQHY GSR F+
Sbjct: 429 YYILGMTATRLAIPLYVFGCPHNFMRVEPNKVWCICLCTFMGLQAVILLLQHYFGSRCFV 488

Query: 496 PRQILPEKYSYYRRLDQNGNHATDCVICMTTIDITR-PNDCMITPCDHFFHSGCLQRWMD 555
           PRQ+LPEKY+Y+RR +++ +  TDCVICMT ID+ +  +DCM+TPC+HFFHSGCLQRWMD
Sbjct: 489 PRQMLPEKYNYHRRFNRDVSRTTDCVICMTAIDLRQHTSDCMVTPCEHFFHSGCLQRWMD 548

Query: 556 IKMECPTCRRLLPPA 570
           IKMECPTCRR LPPA
Sbjct: 549 IKMECPTCRRSLPPA 562

BLAST of MS014022 vs. TAIR 10
Match: AT4G35480.1 (RING-H2 finger A3B )

HSP 1 Score: 54.7 bits (130), Expect = 2.8e-07
Identity = 21/57 (36.84%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 513 NGNHATDCVICMTTIDITRPNDCMITP-CDHFFHSGCLQRWMDIKMECPTCRRLLPP 569
           +G+ +T+C IC+T  + +   +  I P C H FH  C+ +W+  +  CP+CRR+L P
Sbjct: 106 DGDSSTECAICIT--EFSEGEEIRILPLCSHAFHVACIDKWLTSRSSCPSCRRILVP 160

BLAST of MS014022 vs. TAIR 10
Match: AT2G35420.1 (RING/U-box superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 2.4e-06
Identity = 23/57 (40.35%), Postives = 30/57 (52.63%), Query Frame = 0

Query: 515 NHATDCVICMTTI---DITRPNDCMITPCDHFFHSGCLQRWMDIKMECPTCRRLLPP 569
           NH T+C IC++     D  R    +IT C H FHS C+  W ++   CP CR  L P
Sbjct: 98  NHGTECAICLSEFSDEDTVR----LITVCRHPFHSNCIDLWFELHKTCPVCRCELDP 150