MS014019 (gene) Bitter gourd (TR) v1

Overview
NameMS014019
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionearly nodulin-like protein 1
Locationscaffold5: 51034 .. 51703 (-)
RNA-Seq ExpressionMS014019
SyntenyMS014019
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTCTTTCTCTGCTTTTCTGGCTCTGGCCGCTTTATTTGCCCTCACGGCGGCGCCGACCTTTTCTGAGGCTTACAAATTCGTCGTCGGCGGCAAAGACGGCTGGGTCTTCAACCCCTCCGAGCCCTTCAACCACTGGGCCGAGCGTAACAGATTTCAAGTCAACGATACTCTCTGTAACCCCACTAAATCAAATCTCACCAAGTTTTCATTTTTATTTCTGGGTTCTTCTTGTTTTTTAATATTATAATAATAATGACTCTGATCTTGTACTGGGTGCAGATTTCAAGTACCAAAATGGGACGGATTCGGTGCTGGTTGTGAGCAAGGACGATTACTTCTCCTGCAACACAAAAAACCCAATTGTTTCGTTCCAAGATGGAGATTCAATCTTCAAATTCGATCGCTCTGGTCCCTTTTACTTCATCTCCGGCAACGCCGATCGCTGCCAGAAGGGCCAAAAGCTCATTGTTGTGGTCCTCGCTCTCACGCATAACCACCACCACCGCGCTCCGCCGACCCCGACTGCAGACTCGCCGGACTCTGCCGGGGGATTCACGGCGCCAGTTCCGGCTCCGGCCAAGAATTCCGCTTTGGGAGGCGCGGCTAATGTATTCAGTTTCGGTGTTATCAGTGCTGTTCTTTTGAGTTCTTTGGTGGGGCTGTCG

mRNA sequence

ATGCCCTCTTTCTCTGCTTTTCTGGCTCTGGCCGCTTTATTTGCCCTCACGGCGGCGCCGACCTTTTCTGAGGCTTACAAATTCGTCGTCGGCGGCAAAGACGGCTGGGTCTTCAACCCCTCCGAGCCCTTCAACCACTGGGCCGAGCGTAACAGATTTCAAGTCAACGATACTCTCTATTTCAAGTACCAAAATGGGACGGATTCGGTGCTGGTTGTGAGCAAGGACGATTACTTCTCCTGCAACACAAAAAACCCAATTGTTTCGTTCCAAGATGGAGATTCAATCTTCAAATTCGATCGCTCTGGTCCCTTTTACTTCATCTCCGGCAACGCCGATCGCTGCCAGAAGGGCCAAAAGCTCATTGTTGTGGTCCTCGCTCTCACGCATAACCACCACCACCGCGCTCCGCCGACCCCGACTGCAGACTCGCCGGACTCTGCCGGGGGATTCACGGCGCCAGTTCCGGCTCCGGCCAAGAATTCCGCTTTGGGAGGCGCGGCTAATGTATTCAGTTTCGGTGTTATCAGTGCTGTTCTTTTGAGTTCTTTGGTGGGGCTGTCG

Coding sequence (CDS)

ATGCCCTCTTTCTCTGCTTTTCTGGCTCTGGCCGCTTTATTTGCCCTCACGGCGGCGCCGACCTTTTCTGAGGCTTACAAATTCGTCGTCGGCGGCAAAGACGGCTGGGTCTTCAACCCCTCCGAGCCCTTCAACCACTGGGCCGAGCGTAACAGATTTCAAGTCAACGATACTCTCTATTTCAAGTACCAAAATGGGACGGATTCGGTGCTGGTTGTGAGCAAGGACGATTACTTCTCCTGCAACACAAAAAACCCAATTGTTTCGTTCCAAGATGGAGATTCAATCTTCAAATTCGATCGCTCTGGTCCCTTTTACTTCATCTCCGGCAACGCCGATCGCTGCCAGAAGGGCCAAAAGCTCATTGTTGTGGTCCTCGCTCTCACGCATAACCACCACCACCGCGCTCCGCCGACCCCGACTGCAGACTCGCCGGACTCTGCCGGGGGATTCACGGCGCCAGTTCCGGCTCCGGCCAAGAATTCCGCTTTGGGAGGCGCGGCTAATGTATTCAGTTTCGGTGTTATCAGTGCTGTTCTTTTGAGTTCTTTGGTGGGGCTGTCG

Protein sequence

MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVLLSSLVGLS
Homology
BLAST of MS014019 vs. NCBI nr
Match: XP_022140349.1 (early nodulin-like protein 1 [Momordica charantia])

HSP 1 Score: 375.2 bits (962), Expect = 3.4e-100
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0

Query: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60
           MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY
Sbjct: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60

Query: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120
           FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK
Sbjct: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120

Query: 121 LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL 180
           LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL
Sbjct: 121 LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL 180

Query: 181 LSSLVGLS 189
           LSSLVGLS
Sbjct: 181 LSSLVGLS 188

BLAST of MS014019 vs. NCBI nr
Match: XP_023534030.1 (early nodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 248.4 bits (633), Expect = 4.8e-62
Identity = 133/195 (68.21%), Postives = 155/195 (79.49%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ +AFLALA L A+ ++   S AYKF VGG+DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAAFLALALLAAMVSS---SHAYKFYVGGRDGWVQNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLA+ H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA GG+ +  
Sbjct: 124 IVVVLAVRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGGSVSAL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 191

BLAST of MS014019 vs. NCBI nr
Match: KAG6604788.1 (Early nodulin-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 246.5 bits (628), Expect = 1.8e-61
Identity = 134/195 (68.72%), Postives = 153/195 (78.46%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ + FLAL AL  L A  + S+AYKF VGG DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAGFLAL-ALALLAAMVSSSDAYKFYVGGGDGWVQNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLAL H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA GG+ +  
Sbjct: 124 IVVVLALRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGGSVSTL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 193

BLAST of MS014019 vs. NCBI nr
Match: XP_022970831.1 (early nodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 246.5 bits (628), Expect = 1.8e-61
Identity = 133/195 (68.21%), Postives = 154/195 (78.97%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ + FLAL AL  L A  + S+AYKF VGG+DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAGFLAL-ALSLLAAMVSSSDAYKFYVGGRDGWVPNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLA+ H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA GG+ +  
Sbjct: 124 IVVVLAVRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGGSVSAL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 193

BLAST of MS014019 vs. NCBI nr
Match: XP_022948052.1 (early nodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 243.4 bits (620), Expect = 1.5e-60
Identity = 133/195 (68.21%), Postives = 152/195 (77.95%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ + FLAL AL  L A  + S+AYKF VGG DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAGFLAL-ALALLAAMVSSSDAYKFYVGGGDGWVQNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLAL H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA G + +  
Sbjct: 124 IVVVLALRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGRSVSAL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 193

BLAST of MS014019 vs. ExPASy Swiss-Prot
Match: Q5JNJ5 (Early nodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ENODL1 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.1e-32
Identity = 79/169 (46.75%), Postives = 104/169 (61.54%), Query Frame = 0

Query: 4   FSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKY 63
           F A L L A+F      + SEAY F  GG+DGWV +P+E FN+WAERNRFQVNDT+ F +
Sbjct: 13  FMAVLGLVAVF------SSSEAYVFYAGGRDGWVVDPAESFNYWAERNRFQVNDTIVFLH 72

Query: 64  QNGT-DSVLVVSKDDYFSCNTKNPIVSFQD---GDSIFKFDRSGPFYFISGNADRCQKGQ 123
            +    SVL V++ D+ +C+T NP+   +D   G S+F+FDRSGPF+FISG+ DRCQKGQ
Sbjct: 73  DDEVGGSVLQVTEGDFDTCSTGNPVQRLEDVAAGRSVFRFDRSGPFFFISGDEDRCQKGQ 132

Query: 124 KLIVVVLALTHNHHHRAPPTPTADSPDSA----------GGFTAPVPAP 159
           KL ++V+A+       AP    A  P S+           G T P P P
Sbjct: 133 KLYIIVMAVRPTKPSEAPEPAGAAGPVSSKSWSWQAFPPAGATTPPPLP 175

BLAST of MS014019 vs. ExPASy Swiss-Prot
Match: Q9T076 (Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.6e-31
Identity = 85/191 (44.50%), Postives = 104/191 (54.45%), Query Frame = 0

Query: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60
           M S S F  +  L +L+   T S A KF VGG   WV NP E +  W+ +NRF V+DTLY
Sbjct: 6   MKSLSFFFTI--LLSLSTLFTISNARKFNVGGSGAWVTNPPENYESWSGKNRFLVHDTLY 65

Query: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120
           F Y  G DSVL V+K DY +CNTKNPI    DGDS    DR GPFYFISGN D C+KGQK
Sbjct: 66  FSYAKGADSVLEVNKADYDACNTKNPIKRVDDGDSEISLDRYGPFYFISGNEDNCKKGQK 125

Query: 121 LIVVVL-----ALTHNHHHRAPPTPT---------ADSPDSAGGFTAPVPAPAKNSALGG 178
           L VVV+     +   + H  AP + T         A SP S+   +     P   +  GG
Sbjct: 126 LNVVVISARIPSTAQSPHAAAPGSSTPGSMTPPGGAHSPKSSSPVSPTTSPPGSTTPPGG 185

BLAST of MS014019 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-25
Identity = 71/160 (44.38%), Postives = 95/160 (59.38%), Query Frame = 0

Query: 12  ALFALTAAPTFSEAYKFVVGGKDG-WVFNPSE--PFNHWAERNRFQVNDTLYFKYQNGTD 71
           A+F+L    + + A +  VGGK G W   PS    F  WA++ RF+V D + F+Y++G D
Sbjct: 15  AIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGKD 74

Query: 72  SVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLAL 131
           SVL V+K+ Y SCNT NP+ ++ DG++  K DRSGPFYFISG    C+KGQKL +VV++ 
Sbjct: 75  SVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLVVISP 134

Query: 132 THNHHHRAP-PTPTADSPDSAGGFTAPVPAPAKNSA-LGG 167
            H+    AP P    D P       A  PAP   S  LGG
Sbjct: 135 RHSVISPAPSPVEFEDGP-------ALAPAPISGSVRLGG 167

BLAST of MS014019 vs. ExPASy Swiss-Prot
Match: Q8LC95 (Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 67/164 (40.85%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 27  KFVVGGK-DGWVF--NPSEPFNHWAERNRFQVNDTLYFKYQNGTDSVLVVSKDDYFSCNT 86
           + +VGGK   W    +PSE  N WAE  RF+V DTL +KY    DSVL V+KD Y +CNT
Sbjct: 25  EIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWKYDEEKDSVLQVTKDAYINCNT 84

Query: 87  KNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLALTHNHHHRAPPTPTAD 146
            NP  ++ +GD+  K +RSGP++FISG+   C +G+KL +VV++    H           
Sbjct: 85  TNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLHIVVMSSRGGH----------- 144

Query: 147 SPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVLLSSLVGL 188
              + G FT   P+PA + AL GA  V      SA  L+  VG+
Sbjct: 145 ---TGGFFTGSSPSPAPSPALLGAPTVAPASGGSASSLTRQVGV 174

BLAST of MS014019 vs. ExPASy Swiss-Prot
Match: Q02917 (Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.6e-18
Identity = 60/166 (36.14%), Postives = 91/166 (54.82%), Query Frame = 0

Query: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKD-GWVFNPSEP--FNHWAERNRFQVND 60
           +P+ S FL + A+  L +    SEA K+VVGG +  W F  S+P   +HWA  +RF++ D
Sbjct: 5   LPNASPFLVMLAMCLLIST---SEAEKYVVGGSEKSWKFPLSKPDSLSHWANSHRFKIGD 64

Query: 61  TLYFKYQNGTDSVLVVSKDDYFSCNTKNPI-VSFQDGDSIFKFDRSGPFYFISGNADRCQ 120
           TL FKY+  T+SV   ++ DY  CNT     + F  G++     + G  +FISGN   CQ
Sbjct: 65  TLIFKYEKRTESVHEGNETDYEGCNTVGKYHIVFNGGNTKVMLTKPGFRHFISGNQSHCQ 124

Query: 121 KGQKLIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNS 163
            G KL V+V++      +   P+P+   P S+    +P P+P  N+
Sbjct: 125 MGLKLAVLVISSNKTKKNLLSPSPSPSPPPSS--LLSPSPSPLPNN 165

BLAST of MS014019 vs. ExPASy TrEMBL
Match: A0A6J1CFH4 (early nodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111011044 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.6e-100
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0

Query: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60
           MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY
Sbjct: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60

Query: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120
           FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK
Sbjct: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120

Query: 121 LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL 180
           LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL
Sbjct: 121 LIVVVLALTHNHHHRAPPTPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSFGVISAVL 180

Query: 181 LSSLVGLS 189
           LSSLVGLS
Sbjct: 181 LSSLVGLS 188

BLAST of MS014019 vs. ExPASy TrEMBL
Match: A0A6J1I1M7 (early nodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469689 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 8.8e-62
Identity = 133/195 (68.21%), Postives = 154/195 (78.97%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ + FLAL AL  L A  + S+AYKF VGG+DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAGFLAL-ALSLLAAMVSSSDAYKFYVGGRDGWVPNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLA+ H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA GG+ +  
Sbjct: 124 IVVVLAVRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGGSVSAL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 193

BLAST of MS014019 vs. ExPASy TrEMBL
Match: A0A6J1G849 (early nodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111451746 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 7.4e-61
Identity = 133/195 (68.21%), Postives = 152/195 (77.95%), Query Frame = 0

Query: 2   PSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYF 61
           P+ + FLAL AL  L A  + S+AYKF VGG DGWV NPSE FNHWAERNRFQVNDTLYF
Sbjct: 4   PTLAGFLAL-ALALLAAMVSSSDAYKFYVGGGDGWVQNPSENFNHWAERNRFQVNDTLYF 63

Query: 62  KYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKL 121
           KY+NGTDSVLVVSK+DYFSCNTKNP+++ +DG+SIFKF  SGPFYFISG+ADRCQKGQ+L
Sbjct: 64  KYKNGTDSVLVVSKEDYFSCNTKNPVITLKDGESIFKFGHSGPFYFISGDADRCQKGQRL 123

Query: 122 IVVVLALTHNHHHRAP----PTPTADSPDSAG------GFTAPVPAPAKNSALGGAANVF 181
           IVVVLAL H   H AP    PTPTA SP++AG      GFTAPVPAPAK+SA G + +  
Sbjct: 124 IVVVLALRHK-QHLAPSPQAPTPTAQSPENAGSPESGVGFTAPVPAPAKSSAFGRSVSAL 183

Query: 182 SFGVISAVLLSSLVG 187
           S GV   VL+S  VG
Sbjct: 184 SLGV---VLVSFFVG 193

BLAST of MS014019 vs. ExPASy TrEMBL
Match: A0A0A0KG54 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G127430 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.1e-51
Identity = 114/180 (63.33%), Postives = 131/180 (72.78%), Query Frame = 0

Query: 24  EAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKYQNGTDSVLVVSKDDYFSCNT 83
           EA KF VGGKDGW  NPSE FNHWAERNRFQVNDTLYFKY+N T+SVLVVSK+DYFSCNT
Sbjct: 24  EARKFYVGGKDGWGLNPSESFNHWAERNRFQVNDTLYFKYKNETESVLVVSKEDYFSCNT 83

Query: 84  KNPIVSFQD--GDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLALTHNHHHR------ 143
           KNP++S  +  G+S+FKF  SGPFYFI+GNAD CQKGQKLIVVVLALTHN HH       
Sbjct: 84  KNPVISLNENNGESVFKFGHSGPFYFITGNADSCQKGQKLIVVVLALTHNKHHHNQTQPP 143

Query: 144 -------APP----TPTADSPDSAGGFTAPVPAPAKNSALGGAANVFSF---GVISAVLL 182
                  APP    +PTA+SP+ +G      PAPAK+SA G    VFS    G+I+ V+L
Sbjct: 144 HSSFPPVAPPSQSQSPTAESPEKSG----TAPAPAKSSASGRGGVVFSLLGVGMINVVVL 199

BLAST of MS014019 vs. ExPASy TrEMBL
Match: A0A5D3C139 (Early nodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G002090 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.3e-49
Identity = 110/178 (61.80%), Postives = 130/178 (73.03%), Query Frame = 0

Query: 24  EAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKYQNGTDSVLVVSKDDYFSCNT 83
           EA KF VGGKDGW  NPSE FNHWAERNRFQVNDTLYFKY+N T+SVLVVSK+DYFSCNT
Sbjct: 10  EARKFYVGGKDGWGLNPSESFNHWAERNRFQVNDTLYFKYKNETESVLVVSKEDYFSCNT 69

Query: 84  KNPIVSFQD--GDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLALTHNHHHR------ 143
           KNP++S ++  G+S+FKF  SGPFYFIS N D CQKGQKLIVVVLALTHN HH+      
Sbjct: 70  KNPVISLKENNGESVFKFGHSGPFYFISENGDSCQKGQKLIVVVLALTHNKHHQAQSQPP 129

Query: 144 -------APP----TPTADSPDSAGGFTAPVPAPAKNSALGGAA-NVFSFGVISAVLL 182
                  APP    +PTA+SP+ +G  TAP PA +  S  GG A ++    +I+ V+L
Sbjct: 130 HSSFPPVAPPSQSLSPTAESPEKSG--TAPAPAKSSASGRGGVAFSLLGVAMINVVVL 185

BLAST of MS014019 vs. TAIR 10
Match: AT4G32490.1 (early nodulin-like protein 4 )

HSP 1 Score: 154.5 bits (389), Expect = 8.7e-38
Identity = 88/212 (41.51%), Postives = 116/212 (54.72%), Query Frame = 0

Query: 7   FLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKYQNG 66
           +L +  L  L  +   S  +KF VGG+DGWV  PSE ++HW+ RNRFQVNDTLYFKY  G
Sbjct: 10  YLMIVMLMGLGFSIELSNGHKFYVGGRDGWVLTPSEDYSHWSHRNRFQVNDTLYFKYVKG 69

Query: 67  TDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVL 126
            DSVL VS+ +Y +CNT +P+ S  DGDS+F   RS PF+F+SGN+  C KGQKL V V+
Sbjct: 70  KDSVLEVSEKEYNTCNTTHPLTSLSDGDSLFLLSRSDPFFFVSGNSGSCLKGQKLAVTVM 129

Query: 127 ALTHNHH---HRAP---------------------------PTPTADSPDSAGGFTAPVP 186
           +  H+ H   H +P                           P PT     S     AP P
Sbjct: 130 STGHHSHTPRHPSPSPSPSASPVRKALLSPAPIPVHKALSSPAPTPGVDPSHSEVLAPAP 189

BLAST of MS014019 vs. TAIR 10
Match: AT4G28365.1 (early nodulin-like protein 3 )

HSP 1 Score: 148.3 bits (373), Expect = 6.3e-36
Identity = 87/194 (44.85%), Postives = 112/194 (57.73%), Query Frame = 0

Query: 4   FSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKY 63
           F  +L    L  L    T S  YKF VGGKDGWV  PSE ++HW+ RNRFQVNDTL+FKY
Sbjct: 7   FDLYLMFVMLMGL--GFTISNGYKFYVGGKDGWVPTPSEDYSHWSHRNRFQVNDTLHFKY 66

Query: 64  QNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIV 123
             G DSVL V++ +Y +CNT +P+ S  DGDS+F    SG ++FISGN+  C KGQKL V
Sbjct: 67  AKGKDSVLEVTEQEYNTCNTTHPLTSLSDGDSLFLLSHSGSYFFISGNSQNCLKGQKLAV 126

Query: 124 VVLALTHNHH---HRAP-PTPTADSPDSAG---------GFTAPVPAPAKNSALGGAANV 183
            VL+  H+ H   H +P P+P      S G            + VPAP   +A   A  V
Sbjct: 127 KVLSTVHHSHSPRHTSPSPSPVHQELSSPGPSPGVEPSSDSNSRVPAPGPATAPNSAGLV 186

Query: 184 FSFGVISAVLLSSL 185
               V+  +++SSL
Sbjct: 187 GPGMVVLVIMISSL 198

BLAST of MS014019 vs. TAIR 10
Match: AT5G53870.1 (early nodulin-like protein 1 )

HSP 1 Score: 146.4 bits (368), Expect = 2.4e-35
Identity = 83/165 (50.30%), Postives = 105/165 (63.64%), Query Frame = 0

Query: 6   AFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLYFKYQN 65
           +FL LA+     +    ++A++F VGG   WV NP E +N WAERNRFQVND+LYFKY  
Sbjct: 11  SFLILASFATFFSV---ADAWRFNVGGNGAWVTNPQENYNTWAERNRFQVNDSLYFKYAK 70

Query: 66  GTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVV 125
           G+DSV  V K D+  CN +NPI +F++G+S+   DRSG FYFISGN D CQKGQKLIVVV
Sbjct: 71  GSDSVQQVMKADFDGCNVRNPIKNFENGESVVTLDRSGAFYFISGNQDHCQKGQKLIVVV 130

Query: 126 LAL-------THNHHHRAPPT--PTADSPDSAGGFTAPVPAPAKN 162
           LA+        H+      PT  P + SP S     AP  AP+K+
Sbjct: 131 LAVRNQPSAPAHSPVPSVSPTQPPKSHSPVSP---VAPASAPSKS 169

BLAST of MS014019 vs. TAIR 10
Match: AT4G27520.1 (early nodulin-like protein 2 )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-32
Identity = 85/191 (44.50%), Postives = 104/191 (54.45%), Query Frame = 0

Query: 1   MPSFSAFLALAALFALTAAPTFSEAYKFVVGGKDGWVFNPSEPFNHWAERNRFQVNDTLY 60
           M S S F  +  L +L+   T S A KF VGG   WV NP E +  W+ +NRF V+DTLY
Sbjct: 6   MKSLSFFFTI--LLSLSTLFTISNARKFNVGGSGAWVTNPPENYESWSGKNRFLVHDTLY 65

Query: 61  FKYQNGTDSVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQK 120
           F Y  G DSVL V+K DY +CNTKNPI    DGDS    DR GPFYFISGN D C+KGQK
Sbjct: 66  FSYAKGADSVLEVNKADYDACNTKNPIKRVDDGDSEISLDRYGPFYFISGNEDNCKKGQK 125

Query: 121 LIVVVL-----ALTHNHHHRAPPTPT---------ADSPDSAGGFTAPVPAPAKNSALGG 178
           L VVV+     +   + H  AP + T         A SP S+   +     P   +  GG
Sbjct: 126 LNVVVISARIPSTAQSPHAAAPGSSTPGSMTPPGGAHSPKSSSPVSPTTSPPGSTTPPGG 185

BLAST of MS014019 vs. TAIR 10
Match: AT2G25060.1 (early nodulin-like protein 14 )

HSP 1 Score: 117.9 bits (294), Expect = 9.0e-27
Identity = 71/160 (44.38%), Postives = 95/160 (59.38%), Query Frame = 0

Query: 12  ALFALTAAPTFSEAYKFVVGGKDG-WVFNPSE--PFNHWAERNRFQVNDTLYFKYQNGTD 71
           A+F+L    + + A +  VGGK G W   PS    F  WA++ RF+V D + F+Y++G D
Sbjct: 15  AIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGKD 74

Query: 72  SVLVVSKDDYFSCNTKNPIVSFQDGDSIFKFDRSGPFYFISGNADRCQKGQKLIVVVLAL 131
           SVL V+K+ Y SCNT NP+ ++ DG++  K DRSGPFYFISG    C+KGQKL +VV++ 
Sbjct: 75  SVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLVVISP 134

Query: 132 THNHHHRAP-PTPTADSPDSAGGFTAPVPAPAKNSA-LGG 167
            H+    AP P    D P       A  PAP   S  LGG
Sbjct: 135 RHSVISPAPSPVEFEDGP-------ALAPAPISGSVRLGG 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140349.13.4e-100100.00early nodulin-like protein 1 [Momordica charantia][more]
XP_023534030.14.8e-6268.21early nodulin-like protein 1 [Cucurbita pepo subsp. pepo][more]
KAG6604788.11.8e-6168.72Early nodulin-like protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970831.11.8e-6168.21early nodulin-like protein 1 [Cucurbita maxima][more]
XP_022948052.11.5e-6068.21early nodulin-like protein 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q5JNJ54.1e-3246.75Early nodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ENODL1 ... [more]
Q9T0761.6e-3144.50Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 S... [more]
Q9SK271.3e-2544.38Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S... [more]
Q8LC951.2e-2340.85Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 S... [more]
Q029172.6e-1836.14Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CFH41.6e-100100.00early nodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111011044 PE=4... [more]
A0A6J1I1M78.8e-6268.21early nodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469689 PE=4 SV... [more]
A0A6J1G8497.4e-6168.21early nodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111451746 PE=4 ... [more]
A0A0A0KG544.1e-5163.33Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G127430... [more]
A0A5D3C1391.3e-4961.80Early nodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
AT4G32490.18.7e-3841.51early nodulin-like protein 4 [more]
AT4G28365.16.3e-3644.85early nodulin-like protein 3 [more]
AT5G53870.12.4e-3550.30early nodulin-like protein 1 [more]
AT4G27520.11.1e-3244.50early nodulin-like protein 2 [more]
AT2G25060.19.0e-2744.38early nodulin-like protein 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 24..129
e-value: 2.1E-34
score: 119.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 25..126
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 36..119
e-value: 2.9E-21
score: 75.3
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 26..127
score: 41.659706
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 14..143
NoneNo IPR availablePANTHERPTHR33021:SF404EARLY NODULIN-LIKE PROTEIN 2coord: 14..143
IPR041846Early nodulin-like protein domainCDDcd11019OsENODL1_likecoord: 26..126
e-value: 8.10031E-59
score: 177.46

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014019.1MS014019.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0009055 electron transfer activity