MS013886 (gene) Bitter gourd (TR) v1

Overview
NameMS013886
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPLAC8 domain-containing protein/DUF2985 domain-containing protein
Locationscaffold607: 608660 .. 610153 (+)
RNA-Seq ExpressionMS013886
SyntenyMS013886
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAAGAGAAGCTTCAATTTCAAATGCTCCATATTATATCCATGATTTCAAAGATGAAGATGATCTTCACGATTCGATATACAAAAGGAAACTACTTGGGGGTGAGAATTCAAAGATTGGCTCTGCAAATGTGCCCTCCCCATCTGCCAAGTTTTTGCAAATAGCAGAGAGAAGGGATGGGATTTCACGGTCTGTTACTTCTTCAGGAGGGATTCTTAGATCGAAAATCAAGTTGAGATCTTCCGTAGGATTTTGTAAAGAGTGGATCAAAAACCCTTTGAACATTGCCCTTCTTTTGTGGATGATGTGCGTCACAGTTTCGGGAGCGGTACTCTTTCTTGTAATGACTGGAATGTTGAACAATCTCTTGCCAAGTAAACCACAGAGAGATGTTTGGTTTGAGGCTAATAACCAAATCCTGACTGCTCTCTTCACTCTCATGTGTTTGTACCATCATCCTAAGCGGATTCACCACTCGATACTTCTGTGTAGGTGGAAACCGAACGATATTCGTACACTGCGAGAAATATACTGCACAAATGGAACTTACAAGCCTCATGAATGGAGACACATGATGGTACTCCTTGTGTTGCTTAACATAAACTGCATTGCCCAATATGCTTTATCTGGCCTTAATTTGAGCTACAAAAAACCTGAGAGGTCAGTTTTTGGAGTTTCAGTCTGCCTTGCTGTTGCAATCTCAGCTGCTGCAGGTGCTGGTTTATACTCCACTTTTGGCCCACTTGGAAAGGAATACGACTGCGATGACAATGTCGAAGAGAGCGTCTCGGTTGCACCAAGAATTGTTGAGAAGCCACAATGGAGAGGGGGGCTGTTCCATTTCTTGGATGACATTAAAACAGCCTGCCTTTCCATCTTCTGCAGCTTCTGTTTATTTGGATGGAACATGGAAAGGCTCAGATTCGGGAACAAATACGTGCATGTCGCGACATTCGTAATCTTCTGCACCGCCCCGCTTTGTTTGTTTGGTTTGGCAGCTAACATTGTTGATCCTTGGTCTGTCAAAGTAGCTCTCAGTTTAATTGGAATTCTGCTTTCCGTGTTTGGGCTGCTCTATGGTGGATACTGGAGGATTCAAATGAGGAAACGGTTCGACTTGCCAAAGAACGACTCGTGTTGCGGGAAACCAAATGTTGCAGACTGTTCACAGTGGCTTTTCTGCTGTTGCTGCTCTCTTGCTCAGGAGGTTAGGACAGCTGATTACTATGAAACAATGGAAGACAACCTATGCAAAAACCGAACCAACGACAACGTAAAGAACAAGATCTTGTCCCCGTTACCTCGGGAAGGTGGAGTTGCTCATGAGTTTAGATCAAACCTGGCTTCTCCAATCTGGGATAATGCAAAATTAACCGAGATGAGAGCCAAAAAATATCAGTATCCAAACAAGTTCTTAGGTGAAACTTATGGAGAAGATCATATTATGAAGCCCCCTGCTCCATCATCAATGCAAAGAGAGGGTGATGAA

mRNA sequence

ATGGATTCAAGAGAAGCTTCAATTTCAAATGCTCCATATTATATCCATGATTTCAAAGATGAAGATGATCTTCACGATTCGATATACAAAAGGAAACTACTTGGGGGTGAGAATTCAAAGATTGGCTCTGCAAATGTGCCCTCCCCATCTGCCAAGTTTTTGCAAATAGCAGAGAGAAGGGATGGGATTTCACGGTCTGTTACTTCTTCAGGAGGGATTCTTAGATCGAAAATCAAGTTGAGATCTTCCGTAGGATTTTGTAAAGAGTGGATCAAAAACCCTTTGAACATTGCCCTTCTTTTGTGGATGATGTGCGTCACAGTTTCGGGAGCGGTACTCTTTCTTGTAATGACTGGAATGTTGAACAATCTCTTGCCAAGTAAACCACAGAGAGATGTTTGGTTTGAGGCTAATAACCAAATCCTGACTGCTCTCTTCACTCTCATGTGTTTGTACCATCATCCTAAGCGGATTCACCACTCGATACTTCTGTGTAGGTGGAAACCGAACGATATTCGTACACTGCGAGAAATATACTGCACAAATGGAACTTACAAGCCTCATGAATGGAGACACATGATGGTACTCCTTGTGTTGCTTAACATAAACTGCATTGCCCAATATGCTTTATCTGGCCTTAATTTGAGCTACAAAAAACCTGAGAGGTCAGTTTTTGGAGTTTCAGTCTGCCTTGCTGTTGCAATCTCAGCTGCTGCAGGTGCTGGTTTATACTCCACTTTTGGCCCACTTGGAAAGGAATACGACTGCGATGACAATGTCGAAGAGAGCGTCTCGGTTGCACCAAGAATTGTTGAGAAGCCACAATGGAGAGGGGGGCTGTTCCATTTCTTGGATGACATTAAAACAGCCTGCCTTTCCATCTTCTGCAGCTTCTGTTTATTTGGATGGAACATGGAAAGGCTCAGATTCGGGAACAAATACGTGCATGTCGCGACATTCGTAATCTTCTGCACCGCCCCGCTTTGTTTGTTTGGTTTGGCAGCTAACATTGTTGATCCTTGGTCTGTCAAAGTAGCTCTCAGTTTAATTGGAATTCTGCTTTCCGTGTTTGGGCTGCTCTATGGTGGATACTGGAGGATTCAAATGAGGAAACGGTTCGACTTGCCAAAGAACGACTCGTGTTGCGGGAAACCAAATGTTGCAGACTGTTCACAGTGGCTTTTCTGCTGTTGCTGCTCTCTTGCTCAGGAGGTTAGGACAGCTGATTACTATGAAACAATGGAAGACAACCTATGCAAAAACCGAACCAACGACAACGTAAAGAACAAGATCTTGTCCCCGTTACCTCGGGAAGGTGGAGTTGCTCATGAGTTTAGATCAAACCTGGCTTCTCCAATCTGGGATAATGCAAAATTAACCGAGATGAGAGCCAAAAAATATCAGTATCCAAACAAGTTCTTAGGTGAAACTTATGGAGAAGATCATATTATGAAGCCCCCTGCTCCATCATCAATGCAAAGAGAGGGTGATGAA

Coding sequence (CDS)

ATGGATTCAAGAGAAGCTTCAATTTCAAATGCTCCATATTATATCCATGATTTCAAAGATGAAGATGATCTTCACGATTCGATATACAAAAGGAAACTACTTGGGGGTGAGAATTCAAAGATTGGCTCTGCAAATGTGCCCTCCCCATCTGCCAAGTTTTTGCAAATAGCAGAGAGAAGGGATGGGATTTCACGGTCTGTTACTTCTTCAGGAGGGATTCTTAGATCGAAAATCAAGTTGAGATCTTCCGTAGGATTTTGTAAAGAGTGGATCAAAAACCCTTTGAACATTGCCCTTCTTTTGTGGATGATGTGCGTCACAGTTTCGGGAGCGGTACTCTTTCTTGTAATGACTGGAATGTTGAACAATCTCTTGCCAAGTAAACCACAGAGAGATGTTTGGTTTGAGGCTAATAACCAAATCCTGACTGCTCTCTTCACTCTCATGTGTTTGTACCATCATCCTAAGCGGATTCACCACTCGATACTTCTGTGTAGGTGGAAACCGAACGATATTCGTACACTGCGAGAAATATACTGCACAAATGGAACTTACAAGCCTCATGAATGGAGACACATGATGGTACTCCTTGTGTTGCTTAACATAAACTGCATTGCCCAATATGCTTTATCTGGCCTTAATTTGAGCTACAAAAAACCTGAGAGGTCAGTTTTTGGAGTTTCAGTCTGCCTTGCTGTTGCAATCTCAGCTGCTGCAGGTGCTGGTTTATACTCCACTTTTGGCCCACTTGGAAAGGAATACGACTGCGATGACAATGTCGAAGAGAGCGTCTCGGTTGCACCAAGAATTGTTGAGAAGCCACAATGGAGAGGGGGGCTGTTCCATTTCTTGGATGACATTAAAACAGCCTGCCTTTCCATCTTCTGCAGCTTCTGTTTATTTGGATGGAACATGGAAAGGCTCAGATTCGGGAACAAATACGTGCATGTCGCGACATTCGTAATCTTCTGCACCGCCCCGCTTTGTTTGTTTGGTTTGGCAGCTAACATTGTTGATCCTTGGTCTGTCAAAGTAGCTCTCAGTTTAATTGGAATTCTGCTTTCCGTGTTTGGGCTGCTCTATGGTGGATACTGGAGGATTCAAATGAGGAAACGGTTCGACTTGCCAAAGAACGACTCGTGTTGCGGGAAACCAAATGTTGCAGACTGTTCACAGTGGCTTTTCTGCTGTTGCTGCTCTCTTGCTCAGGAGGTTAGGACAGCTGATTACTATGAAACAATGGAAGACAACCTATGCAAAAACCGAACCAACGACAACGTAAAGAACAAGATCTTGTCCCCGTTACCTCGGGAAGGTGGAGTTGCTCATGAGTTTAGATCAAACCTGGCTTCTCCAATCTGGGATAATGCAAAATTAACCGAGATGAGAGCCAAAAAATATCAGTATCCAAACAAGTTCTTAGGTGAAACTTATGGAGAAGATCATATTATGAAGCCCCCTGCTCCATCATCAATGCAAAGAGAGGGTGATGAA

Protein sequence

MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERRDGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGMLNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYCTNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAGAGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGEDHIMKPPAPSSMQREGDE
Homology
BLAST of MS013886 vs. NCBI nr
Match: XP_022143996.1 (uncharacterized protein LOC111013784 [Momordica charantia] >XP_022143997.1 uncharacterized protein LOC111013784 [Momordica charantia])

HSP 1 Score: 1033.1 bits (2670), Expect = 7.9e-298
Identity = 496/498 (99.60%), Postives = 497/498 (99.80%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR
Sbjct: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM
Sbjct: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC
Sbjct: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
           TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG
Sbjct: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSIFCSFCL 300
           AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDI TACLSIFCSFCL
Sbjct: 241 AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDIXTACLSIFCSFCL 300

Query: 301 FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSVFGLL 360
           FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLS+FGLL
Sbjct: 301 FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSMFGLL 360

Query: 361 YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK 420
           YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK
Sbjct: 361 YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK 420

Query: 421 NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE 480
           NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE
Sbjct: 421 NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE 480

Query: 481 DHIMKPPAPSSMQREGDE 499
           DHIMKPPAPSSMQREGDE
Sbjct: 481 DHIMKPPAPSSMQREGDE 498

BLAST of MS013886 vs. NCBI nr
Match: XP_038880872.1 (uncharacterized protein LOC120072556 [Benincasa hispida])

HSP 1 Score: 812.8 bits (2098), Expect = 1.7e-231
Identity = 394/504 (78.17%), Postives = 433/504 (85.91%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDSRE S  ++ YY H  K  D+L   I +R LLG E+SKIGSAN+PSPSAKF +IA+R 
Sbjct: 1   MDSREPSTLSSAYYEHGSKCNDELRGLISQRNLLGDEDSKIGSANLPSPSAKFQKIADRM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS+SV SS GIL   IKLRSS+ F KEWIK+PLNIA LLWMMCV +SGAVLFLVMTGM
Sbjct: 61  DEISQSVPSSRGILEQNIKLRSSIAFWKEWIKDPLNIAFLLWMMCVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK QR+VWFE NNQILTALFTLMCLYHHPKRIHH ILLCRWKP+DI  LR++YC
Sbjct: 121 LNNLLPSKSQREVWFEVNNQILTALFTLMCLYHHPKRIHHVILLCRWKPSDICILRKMYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKPHEW+HMMVLL+LL+INC AQYALSGLNLSYKKPERSVFGVS+CLAVAI +AAG
Sbjct: 181 KNGTYKPHEWKHMMVLLLLLHINCFAQYALSGLNLSYKKPERSVFGVSICLAVAIISAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY+CD++      +EES+S A RIV+KPQWRGGLFHFLDDIKTA LS+
Sbjct: 241 AGLYSIFSPLGKEYNCDEDEENPNRIEESISFASRIVDKPQWRGGLFHFLDDIKTAFLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH ATFVIFCTAPLCLFGLAANIVDPWSVK+A SL GILL
Sbjct: 301 FCSFCLFGWNMERLGFGNLYVHAATFVIFCTAPLCLFGLAANIVDPWSVKIAFSLTGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+SC GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSCWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           E+NLCKNRTNDN  NKILSPLPREGG  HE RSNLASPIWD+ KLTE+ AKK     + L
Sbjct: 421 EENLCKNRTNDNDGNKILSPLPREGGTIHELRSNLASPIWDSVKLTELMAKKDLNSIRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET G + +M PP+PSSMQR+G E
Sbjct: 481 YETDGVEQLMNPPSPSSMQRDGFE 504

BLAST of MS013886 vs. NCBI nr
Match: XP_004149932.2 (uncharacterized protein LOC101211290 [Cucumis sativus] >KGN54428.1 hypothetical protein Csa_012538 [Cucumis sativus])

HSP 1 Score: 776.9 bits (2005), Expect = 1.0e-220
Identity = 379/500 (75.80%), Postives = 418/500 (83.60%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SKIGSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSAAYYEHEDKYHDELHRSISQRNLLRSEDSKIGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           DG S SV SS GIL  KIKLRSS+ FCKEWIK+PLNIALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DGSSWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNIALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLP+K +RDVWFE NNQ LTALFTLMCLYHHPKRIHH I+LCRWKP DI  LR++YC
Sbjct: 121 LNNLLPNKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVIVLCRWKPGDILMLRKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDC--DDN---VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSIF 300
           AGLYS F PLGK+Y    D+N   ++ES+S   RIV+KPQWRGGLFHFLDDIKTACLS+F
Sbjct: 241 AGLYSIFSPLGKDYSPSEDENPNRIKESISFESRIVDKPQWRGGLFHFLDDIKTACLSLF 300

Query: 301 CSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLS 360
           CSFCLFGWNMERL FGN YVHV TFVIFC APLCLFGLAAN VDPWSVKVA  LIGILLS
Sbjct: 301 CSFCLFGWNMERLGFGNMYVHVTTFVIFCFAPLCLFGLAANTVDPWSVKVAFCLIGILLS 360

Query: 361 VFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETME 420
           VFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM+
Sbjct: 361 VFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETMK 420

Query: 421 DNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLG 480
           DNLCKNRTND  KN++LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L 
Sbjct: 421 DNLCKNRTNDADKNEVLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLLD 480

Query: 481 ETYGEDHIMKPPAPSSMQRE 496
           E+   + +M PP  SSMQR+
Sbjct: 481 ESDEVEQLMSPPTLSSMQRD 500

BLAST of MS013886 vs. NCBI nr
Match: TYK10972.1 (PLAC8 domain-containing protein/DUF2985 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 773.9 bits (1997), Expect = 8.6e-220
Identity = 377/504 (74.80%), Postives = 417/504 (82.74%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SK+GSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSASYYEHEDKYHDELHRSISQRNLLRSEDSKVGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS SV SS GIL  KIKLRSS+ FCKEWIK+PLN+ALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DEISWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNVALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK +RDVWFE NNQ LTALFTLMCLYHHPKRIHH ILLCRWKP DI TLR++YC
Sbjct: 121 LNNLLPSKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVILLCRWKPGDILTLRKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY   ++      ++ES+S A RI +KPQWRG LFHFLDDIKTACLS+
Sbjct: 241 AGLYSIFSPLGKEYSPGEDEKNPNQIKESISFASRIADKPQWRGELFHFLDDIKTACLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH+ TFVIFC+APLCLFGLAAN VDPWSVKVA  LIGILL
Sbjct: 301 FCSFCLFGWNMERLGFGNTYVHITTFVIFCSAPLCLFGLAANTVDPWSVKVAFCLIGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           +DNLCKNRT+   KN+ LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L
Sbjct: 421 KDNLCKNRTSHADKNEDLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET   + +M PP  +SMQR G E
Sbjct: 481 DETDEVEQLMNPPTIASMQRNGFE 504

BLAST of MS013886 vs. NCBI nr
Match: XP_008464250.1 (PREDICTED: uncharacterized protein LOC103502180 isoform X1 [Cucumis melo] >XP_016903162.1 PREDICTED: uncharacterized protein LOC103502180 isoform X1 [Cucumis melo] >KAA0065328.1 PLAC8 domain-containing protein/DUF2985 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 772.3 bits (1993), Expect = 2.5e-219
Identity = 376/504 (74.60%), Postives = 417/504 (82.74%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SK+GSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSASYYEHEDKYHDELHRSISQRNLLRSEDSKVGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS SV SS GIL  KIKLRSS+ FCKEWIK+PLN+ALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DEISWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNVALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK +RDVWFE NNQ LTALFTLMCLYHHPKRIHH ILLCRWKP DI TL+++YC
Sbjct: 121 LNNLLPSKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVILLCRWKPGDILTLQKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY   ++      ++ES+S A RI +KPQWRG LFHFLDDIKTACLS+
Sbjct: 241 AGLYSIFSPLGKEYSPGEDEKNPNQIKESISFASRIADKPQWRGELFHFLDDIKTACLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH+ TFVIFC+APLCLFGLAAN VDPWSVKVA  LIGILL
Sbjct: 301 FCSFCLFGWNMERLGFGNTYVHITTFVIFCSAPLCLFGLAANTVDPWSVKVAFCLIGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           +DNLCKNRT+   KN+ LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L
Sbjct: 421 KDNLCKNRTSHADKNEDLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET   + +M PP  +SMQR G E
Sbjct: 481 DETDEVEQLMNPPTIASMQRNGFE 504

BLAST of MS013886 vs. ExPASy Swiss-Prot
Match: Q9LS43 (Protein PLANT CADMIUM RESISTANCE 7 OS=Arabidopsis thaliana OX=3702 GN=PCR7 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.1e-04
Identity = 38/136 (27.94%), Postives = 55/136 (40.44%), Query Frame = 0

Query: 275 QWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLA 334
           QW  GLF  ++D +TACL+ FC                              P   FG  
Sbjct: 4   QWTSGLFSCMEDSETACLTCFC------------------------------PCVTFGRI 63

Query: 335 ANIVDPWSVKVALS--LIGILLSVFGL--LYGGYWRIQMRKRFDLPKNDSCCGKPNVADC 394
           A+I D             G++  V GL  L+   +R ++R +F LP++ +       +DC
Sbjct: 64  ADISDEGRTGCGRCGVFYGLICCVVGLPCLFSCTYRTKIRSKFGLPESPT-------SDC 102

Query: 395 SQWLFCCCCSLAQEVR 407
               FC CC+L QE R
Sbjct: 124 VTHFFCECCALCQEHR 102

BLAST of MS013886 vs. ExPASy TrEMBL
Match: A0A6J1CRZ7 (uncharacterized protein LOC111013784 OS=Momordica charantia OX=3673 GN=LOC111013784 PE=4 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 3.8e-298
Identity = 496/498 (99.60%), Postives = 497/498 (99.80%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR
Sbjct: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM
Sbjct: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC
Sbjct: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
           TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG
Sbjct: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSIFCSFCL 300
           AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDI TACLSIFCSFCL
Sbjct: 241 AGLYSTFGPLGKEYDCDDNVEESVSVAPRIVEKPQWRGGLFHFLDDIXTACLSIFCSFCL 300

Query: 301 FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSVFGLL 360
           FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLS+FGLL
Sbjct: 301 FGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSMFGLL 360

Query: 361 YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK 420
           YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK
Sbjct: 361 YGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCK 420

Query: 421 NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE 480
           NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE
Sbjct: 421 NRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGETYGE 480

Query: 481 DHIMKPPAPSSMQREGDE 499
           DHIMKPPAPSSMQREGDE
Sbjct: 481 DHIMKPPAPSSMQREGDE 498

BLAST of MS013886 vs. ExPASy TrEMBL
Match: A0A0A0KY50 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G325530 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.9e-221
Identity = 379/500 (75.80%), Postives = 418/500 (83.60%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SKIGSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSAAYYEHEDKYHDELHRSISQRNLLRSEDSKIGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           DG S SV SS GIL  KIKLRSS+ FCKEWIK+PLNIALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DGSSWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNIALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLP+K +RDVWFE NNQ LTALFTLMCLYHHPKRIHH I+LCRWKP DI  LR++YC
Sbjct: 121 LNNLLPNKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVIVLCRWKPGDILMLRKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDC--DDN---VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSIF 300
           AGLYS F PLGK+Y    D+N   ++ES+S   RIV+KPQWRGGLFHFLDDIKTACLS+F
Sbjct: 241 AGLYSIFSPLGKDYSPSEDENPNRIKESISFESRIVDKPQWRGGLFHFLDDIKTACLSLF 300

Query: 301 CSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLS 360
           CSFCLFGWNMERL FGN YVHV TFVIFC APLCLFGLAAN VDPWSVKVA  LIGILLS
Sbjct: 301 CSFCLFGWNMERLGFGNMYVHVTTFVIFCFAPLCLFGLAANTVDPWSVKVAFCLIGILLS 360

Query: 361 VFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETME 420
           VFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM+
Sbjct: 361 VFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETMK 420

Query: 421 DNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLG 480
           DNLCKNRTND  KN++LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L 
Sbjct: 421 DNLCKNRTNDADKNEVLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLLD 480

Query: 481 ETYGEDHIMKPPAPSSMQRE 496
           E+   + +M PP  SSMQR+
Sbjct: 481 ESDEVEQLMSPPTLSSMQRD 500

BLAST of MS013886 vs. ExPASy TrEMBL
Match: A0A5D3CI52 (PLAC8 domain-containing protein/DUF2985 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00600 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.2e-220
Identity = 377/504 (74.80%), Postives = 417/504 (82.74%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SK+GSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSASYYEHEDKYHDELHRSISQRNLLRSEDSKVGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS SV SS GIL  KIKLRSS+ FCKEWIK+PLN+ALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DEISWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNVALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK +RDVWFE NNQ LTALFTLMCLYHHPKRIHH ILLCRWKP DI TLR++YC
Sbjct: 121 LNNLLPSKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVILLCRWKPGDILTLRKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY   ++      ++ES+S A RI +KPQWRG LFHFLDDIKTACLS+
Sbjct: 241 AGLYSIFSPLGKEYSPGEDEKNPNQIKESISFASRIADKPQWRGELFHFLDDIKTACLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH+ TFVIFC+APLCLFGLAAN VDPWSVKVA  LIGILL
Sbjct: 301 FCSFCLFGWNMERLGFGNTYVHITTFVIFCSAPLCLFGLAANTVDPWSVKVAFCLIGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           +DNLCKNRT+   KN+ LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L
Sbjct: 421 KDNLCKNRTSHADKNEDLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET   + +M PP  +SMQR G E
Sbjct: 481 DETDEVEQLMNPPTIASMQRNGFE 504

BLAST of MS013886 vs. ExPASy TrEMBL
Match: A0A1S3CMK5 (uncharacterized protein LOC103502180 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502180 PE=4 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.2e-219
Identity = 376/504 (74.60%), Postives = 417/504 (82.74%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SK+GSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSASYYEHEDKYHDELHRSISQRNLLRSEDSKVGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS SV SS GIL  KIKLRSS+ FCKEWIK+PLN+ALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DEISWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNVALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK +RDVWFE NNQ LTALFTLMCLYHHPKRIHH ILLCRWKP DI TL+++YC
Sbjct: 121 LNNLLPSKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVILLCRWKPGDILTLQKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY   ++      ++ES+S A RI +KPQWRG LFHFLDDIKTACLS+
Sbjct: 241 AGLYSIFSPLGKEYSPGEDEKNPNQIKESISFASRIADKPQWRGELFHFLDDIKTACLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH+ TFVIFC+APLCLFGLAAN VDPWSVKVA  LIGILL
Sbjct: 301 FCSFCLFGWNMERLGFGNTYVHITTFVIFCSAPLCLFGLAANTVDPWSVKVAFCLIGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           +DNLCKNRT+   KN+ LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L
Sbjct: 421 KDNLCKNRTSHADKNEDLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET   + +M PP  +SMQR G E
Sbjct: 481 DETDEVEQLMNPPTIASMQRNGFE 504

BLAST of MS013886 vs. ExPASy TrEMBL
Match: A0A5A7VCG4 (PLAC8 domain-containing protein/DUF2985 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3189G00030 PE=4 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.2e-219
Identity = 376/504 (74.60%), Postives = 417/504 (82.74%), Query Frame = 0

Query: 1   MDSREASISNAPYYIHDFKDEDDLHDSIYKRKLLGGENSKIGSANVPSPSAKFLQIAERR 60
           MDS+  S  +A YY H+ K  D+LH SI +R LL  E+SK+GSANVPSPSAKF  + ER 
Sbjct: 1   MDSKAPSTLSASYYEHEDKYHDELHRSISQRNLLRSEDSKVGSANVPSPSAKFQNMVERM 60

Query: 61  DGISRSVTSSGGILRSKIKLRSSVGFCKEWIKNPLNIALLLWMMCVTVSGAVLFLVMTGM 120
           D IS SV SS GIL  KIKLRSS+ FCKEWIK+PLN+ALLLWM+CV +SGAVLFLVMTGM
Sbjct: 61  DEISWSVPSSRGILEQKIKLRSSITFCKEWIKDPLNVALLLWMICVAISGAVLFLVMTGM 120

Query: 121 LNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSILLCRWKPNDIRTLREIYC 180
           LNNLLPSK +RDVWFE NNQ LTALFTLMCLYHHPKRIHH ILLCRWKP DI TL+++YC
Sbjct: 121 LNNLLPSKSRRDVWFEVNNQFLTALFTLMCLYHHPKRIHHVILLCRWKPGDILTLQKVYC 180

Query: 181 TNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPERSVFGVSVCLAVAISAAAG 240
            NGTYKP+EW+HMMVLL+LL+INC AQYALS LNL YKKPERSVFGVS+CLAVAI AAAG
Sbjct: 181 KNGTYKPNEWKHMMVLLLLLHINCFAQYALSSLNLRYKKPERSVFGVSICLAVAILAAAG 240

Query: 241 AGLYSTFGPLGKEYDCDDN------VEESVSVAPRIVEKPQWRGGLFHFLDDIKTACLSI 300
           AGLYS F PLGKEY   ++      ++ES+S A RI +KPQWRG LFHFLDDIKTACLS+
Sbjct: 241 AGLYSIFSPLGKEYSPGEDEKNPNQIKESISFASRIADKPQWRGELFHFLDDIKTACLSL 300

Query: 301 FCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILL 360
           FCSFCLFGWNMERL FGN YVH+ TFVIFC+APLCLFGLAAN VDPWSVKVA  LIGILL
Sbjct: 301 FCSFCLFGWNMERLGFGNTYVHITTFVIFCSAPLCLFGLAANTVDPWSVKVAFCLIGILL 360

Query: 361 SVFGLLYGGYWRIQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETM 420
           SVFGLLYGGYWRIQMRKRFDLPKN+S  GKPNVADC+QWLFCCCCSLAQEVRTADYYETM
Sbjct: 361 SVFGLLYGGYWRIQMRKRFDLPKNNSFWGKPNVADCAQWLFCCCCSLAQEVRTADYYETM 420

Query: 421 EDNLCKNRTNDNVKNKILSPLPREGGVAHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFL 480
           +DNLCKNRT+   KN+ LSPLPREG   H  RSNLASPIWD+ KLTE  AKK    N+ L
Sbjct: 421 KDNLCKNRTSHADKNEDLSPLPREGRTVHGLRSNLASPIWDSVKLTETMAKKDLNSNRLL 480

Query: 481 GETYGEDHIMKPPAPSSMQREGDE 499
            ET   + +M PP  +SMQR G E
Sbjct: 481 DETDEVEQLMNPPTIASMQRNGFE 504

BLAST of MS013886 vs. TAIR 10
Match: AT5G05350.1 (PLAC8 family protein )

HSP 1 Score: 478.4 bits (1230), Expect = 7.0e-135
Identity = 243/477 (50.94%), Postives = 309/477 (64.78%), Query Frame = 0

Query: 48  SPSAKFLQIAERRDGISRSVTSSGGILRSKIKLRSSVGFC-----KEWIKNPLNIALLLW 107
           SPSA+  ++AE RD +SRSV SS       +  R     C     K+W++NPLN+ + LW
Sbjct: 55  SPSARLKRMAEERDEVSRSVNSSSSSSHDVVSSRKIEWACLMKMGKQWLQNPLNMVMFLW 114

Query: 108 MMCVTVSGAVLFLVMTGMLNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHPKRIHHSI 167
           +  V VSGA+LF+VMTGMLN+ LP K QRDVWFE NNQIL ALFTLMCLY HPKR +H +
Sbjct: 115 IFVVAVSGAILFMVMTGMLNHALPKKSQRDVWFEVNNQILNALFTLMCLYQHPKRFYHLV 174

Query: 168 LLCRWKPNDIRTLREIYCTNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNLSYKKPER 227
           LLCRWK +D+ TLR+I+C NGTYKP+EW HMMV+++LL++NC AQYAL GLNL Y++ ER
Sbjct: 175 LLCRWKQDDVTTLRKIFCKNGTYKPNEWIHMMVVVLLLHLNCFAQYALCGLNLGYRRSER 234

Query: 228 SVFGVSVCLAVAISAAAGAGLYSTFGPLGKEYDCDDNVEESV------------------ 287
              GV++C++ AI+A   AGLY+   PLGK+YD   + E  V                  
Sbjct: 235 PAIGVAICISFAIAAPTAAGLYTILSPLGKDYDPQGDEENQVQAVGEGPVSNRKLSLERR 294

Query: 288 -SVAPRIVEKPQWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVATFVIF 347
            S A   V  P+WR G+    +DI  A LS+FC+FCLFGWNMER+ FGN YVH+ATFV+F
Sbjct: 295 YSFASNDVSNPEWREGVLDIWEDISLAYLSLFCTFCLFGWNMERIGFGNMYVHIATFVLF 354

Query: 348 CTAPLCLFGLAANIVDPWSVKVALSLIGILLSVFGLLYGGYWRIQMRKRFDLPKNDSCCG 407
           C AP  +F LAA  +D   V+ AL   GI+L +FGLLYGG+WRIQMRKRF LP  + CCG
Sbjct: 355 CLAPFFIFNLAAINIDNEMVRDALGYTGIVLCLFGLLYGGFWRIQMRKRFKLPGYNICCG 414

Query: 408 KPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCKNR-TNDNVKNK-ILSPLPREGGV 467
           +P +ADC+ WLFCC CSLAQEVRTA+ YE +ED  CK R  N  + ++ ++S LPRE GV
Sbjct: 415 RPAIADCTLWLFCCWCSLAQEVRTANSYEIVEDKFCKRREENSKIDDEVVVSALPREDGV 474

Query: 468 AHEFRSNLASPIWDNAKLTEMRAKKYQYPNKFLGET-YGE--DHIMKPPAPSSMQRE 496
                             T M A     P++   ET  GE  D  + PP+P  + RE
Sbjct: 475 -------FDPSCLPKKMTTTMIASSTLSPSRQKDETCLGEKGDEALSPPSPPFIHRE 524

BLAST of MS013886 vs. TAIR 10
Match: AT3G10980.1 (PLAC8 family protein )

HSP 1 Score: 471.5 bits (1212), Expect = 8.5e-133
Identity = 244/503 (48.51%), Postives = 316/503 (62.82%), Query Frame = 0

Query: 48  SPSAKFLQIAERRDGISRSVTSS------------GGILRSKIKLRSSVGFCKEWIKNPL 107
           SPSAKF ++AE RD +SRSVTSS             G+L  KI   S +   KEWI+NP+
Sbjct: 68  SPSAKFKKMAEDRDEVSRSVTSSSNGSSHNFRERISGVLHRKIDWSSLMNMGKEWIRNPI 127

Query: 108 NIALLLWMMCVTVSGAVLFLVMTGMLNNLLPSKPQRDVWFEANNQILTALFTLMCLYHHP 167
           N+AL +W++ V VSGA+LF+VMTGMLN+ LP K QRD WFE NNQIL  LFTLMCLY HP
Sbjct: 128 NMALFVWILVVAVSGAILFMVMTGMLNHALPKKSQRDAWFEVNNQILNGLFTLMCLYQHP 187

Query: 168 KRIHHSILLCRWKPNDIRTLREIYCTNGTYKPHEWRHMMVLLVLLNINCIAQYALSGLNL 227
           KR +H +LLCRWK +DI  LR+ YC +GTYKP+EW H+MV+++LL++NC AQYAL GLN+
Sbjct: 188 KRFYHLVLLCRWKHDDITKLRKAYCKDGTYKPNEWMHIMVVVILLHLNCFAQYALCGLNV 247

Query: 228 SYKKPERSVFGVSVCLAVAISAAAGAGLYSTFGPLGKEYDCDDNVEESVSVAPR------ 287
            Y++ ER   GV++C++ AI A A AGLY+   PLGK+Y+ D N +E   +  R      
Sbjct: 248 GYRRSERPPIGVAICISAAIGAPAVAGLYTILSPLGKDYN-DSNEDEENQLKQREEGSVN 307

Query: 288 -----------------------IVEKPQWRGGLFHFLDDIKTACLSIFCSFCLFGWNME 347
                                   V  PQW GG+    DDI  A LS+FC+FC+FGWNME
Sbjct: 308 RRFTLERRYSFASASTGVGDGMVPVSDPQWSGGILDIWDDISLAYLSLFCTFCVFGWNME 367

Query: 348 RLRFGNKYVHVATFVIFCTAPLCLFGLAANIVDPWSVKVALSLIGILLSVFGLLYGGYWR 407
           R+ FGN YVH+ATF++FC AP  +F LAA  +D  +V+ AL + GILL VFGLLYGG+WR
Sbjct: 368 RVGFGNMYVHIATFILFCLAPFFIFNLAAVNIDNETVREALGISGILLCVFGLLYGGFWR 427

Query: 408 IQMRKRFDLPKNDSCCGKPNVADCSQWLFCCCCSLAQEVRTADYYETMEDNLCKNRTNDN 467
           IQMRKRF LP  + C G+  +ADC+ WL CC CSLAQEVRTA+ YE +ED  C+ +   N
Sbjct: 428 IQMRKRFKLPSYNFCFGRAAIADCALWLCCCWCSLAQEVRTANSYEIVEDKFCQRKEEKN 487

Query: 468 -VKNKILSPLPREGGV---------AHEFRSNLASP----IWDNAKLTEMRAKKYQYPNK 496
            V   ++SPLPRE GV         + +  S  +SP     W  A    ++  + +    
Sbjct: 488 MVSPNLVSPLPREDGVFDPRFGLGSSPKNISGASSPSPSRFWKEAHSPNVQTPREK---- 547

BLAST of MS013886 vs. TAIR 10
Match: AT3G18470.1 (PLAC8 family protein )

HSP 1 Score: 49.7 bits (117), Expect = 8.0e-06
Identity = 38/136 (27.94%), Postives = 55/136 (40.44%), Query Frame = 0

Query: 275 QWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVATFVIFCTAPLCLFGLA 334
           QW  GLF  ++D +TACL+ FC                              P   FG  
Sbjct: 4   QWTSGLFSCMEDSETACLTCFC------------------------------PCVTFGRI 63

Query: 335 ANIVDPWSVKVALS--LIGILLSVFGL--LYGGYWRIQMRKRFDLPKNDSCCGKPNVADC 394
           A+I D             G++  V GL  L+   +R ++R +F LP++ +       +DC
Sbjct: 64  ADISDEGRTGCGRCGVFYGLICCVVGLPCLFSCTYRTKIRSKFGLPESPT-------SDC 102

Query: 395 SQWLFCCCCSLAQEVR 407
               FC CC+L QE R
Sbjct: 124 VTHFFCECCALCQEHR 102

BLAST of MS013886 vs. TAIR 10
Match: AT2G45010.1 (PLAC8 family protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.0e-04
Identity = 40/155 (25.81%), Postives = 64/155 (41.29%), Query Frame = 0

Query: 262 ESVSVAPRIVEKP---QWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVA 321
           E + V P   E P    W  G+F   +D ++    +FC   LFG N+E +R    +    
Sbjct: 45  ECMQVLPETYEPPSDENWTTGIFGCAEDPESCRTGLFCPCVLFGRNIEAVREEIPWTQPC 104

Query: 322 TFVIFCT----APLCLFGLAANIVDPWSVKVALSLIGILLSVFGLLYGGYWRIQMRKRFD 381
                C     A   +  L +  +DP +  V    +     + G +Y G +R +++K++ 
Sbjct: 105 VCHAVCVEGGMALAAVTALFSGYIDPQTTVVICEGLFFAWWMCG-IYSGLFRQELQKKYH 164

Query: 382 LPKNDSCCGKPNVADCSQWLFCCC---CSLAQEVR 407
           L KN         A C   +  CC   C+L QE R
Sbjct: 165 L-KN---------APCDHCMVHCCLHWCALCQEHR 188

BLAST of MS013886 vs. TAIR 10
Match: AT2G45010.2 (PLAC8 family protein )

HSP 1 Score: 43.1 bits (100), Expect = 7.5e-04
Identity = 39/153 (25.49%), Postives = 63/153 (41.18%), Query Frame = 0

Query: 264 VSVAPRIVEKP---QWRGGLFHFLDDIKTACLSIFCSFCLFGWNMERLRFGNKYVHVATF 323
           + V P   E P    W  G+F   +D ++    +FC   LFG N+E +R    +      
Sbjct: 1   MQVLPETYEPPSDENWTTGIFGCAEDPESCRTGLFCPCVLFGRNIEAVREEIPWTQPCVC 60

Query: 324 VIFCT----APLCLFGLAANIVDPWSVKVALSLIGILLSVFGLLYGGYWRIQMRKRFDLP 383
              C     A   +  L +  +DP +  V    +     + G +Y G +R +++K++ L 
Sbjct: 61  HAVCVEGGMALAAVTALFSGYIDPQTTVVICEGLFFAWWMCG-IYSGLFRQELQKKYHL- 120

Query: 384 KNDSCCGKPNVADCSQWLFCCC---CSLAQEVR 407
           KN         A C   +  CC   C+L QE R
Sbjct: 121 KN---------APCDHCMVHCCLHWCALCQEHR 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143996.17.9e-29899.60uncharacterized protein LOC111013784 [Momordica charantia] >XP_022143997.1 uncha... [more]
XP_038880872.11.7e-23178.17uncharacterized protein LOC120072556 [Benincasa hispida][more]
XP_004149932.21.0e-22075.80uncharacterized protein LOC101211290 [Cucumis sativus] >KGN54428.1 hypothetical ... [more]
TYK10972.18.6e-22074.80PLAC8 domain-containing protein/DUF2985 domain-containing protein [Cucumis melo ... [more]
XP_008464250.12.5e-21974.60PREDICTED: uncharacterized protein LOC103502180 isoform X1 [Cucumis melo] >XP_01... [more]
Match NameE-valueIdentityDescription
Q9LS431.1e-0427.94Protein PLANT CADMIUM RESISTANCE 7 OS=Arabidopsis thaliana OX=3702 GN=PCR7 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CRZ73.8e-29899.60uncharacterized protein LOC111013784 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A0A0KY504.9e-22175.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G325530 PE=4 SV=1[more]
A0A5D3CI524.2e-22074.80PLAC8 domain-containing protein/DUF2985 domain-containing protein OS=Cucumis mel... [more]
A0A1S3CMK51.2e-21974.60uncharacterized protein LOC103502180 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7VCG41.2e-21974.60PLAC8 domain-containing protein/DUF2985 domain-containing protein OS=Cucumis mel... [more]
Match NameE-valueIdentityDescription
AT5G05350.17.0e-13550.94PLAC8 family protein [more]
AT3G10980.18.5e-13348.51PLAC8 family protein [more]
AT3G18470.18.0e-0627.94PLAC8 family protein [more]
AT2G45010.12.0e-0425.81PLAC8 family protein [more]
AT2G45010.27.5e-0425.49PLAC8 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006461PLAC8 motif-containing proteinTIGRFAMTIGR01571TIGR01571coord: 275..408
e-value: 2.6E-25
score: 87.2
IPR006461PLAC8 motif-containing proteinPFAMPF04749PLAC8coord: 276..406
e-value: 2.4E-17
score: 63.6
IPR021369Protein of unknown function DUF2985PFAMPF11204DUF2985coord: 89..167
e-value: 9.1E-26
score: 89.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 478..498
NoneNo IPR availablePANTHERPTHR31045PLAC8 FAMILY PROTEIN-RELATEDcoord: 44..495
NoneNo IPR availablePANTHERPTHR31045:SF21SUBFAMILY NOT NAMEDcoord: 44..495

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013886.1MS013886.1mRNA