MS013872 (gene) Bitter gourd (TR) v1

Overview


Name	MS013872
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	proactivator polypeptide-like 1
Location	scaffold607: 488774 .. 492081 (-)
RNA-Seq Expression	MS013872
Synteny	MS013872

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGGTAAATCTTTATTTTTCATTTTTGATCGATATGTCTGATTGTTGTTTAGAATTGGATAAATATGTAACGTAATCTAGGCCGTAATCTCAATTTCTTCTCTAATTTTTTGGGAAATTCAAATTTATCTTTTTTGTGTATTGATTTTTCCCCCATGAAATTTAGATTGAAACGAATTTAAATAGAGTATTTTGGTCATGAGGTTTTATGAGTCTAATTTCCACAAGCCATTTCAAACCGAATAAGTTTCTGAATTTATGGATGAGCTTGTCTATTTCATCCCATAAAAAGGTAAAGTATGGGTATTTGTTAATTAATAAAATGAATTTTGATGCTACATCGTCATTTTGTCAATGAATTTTCACATTTTGACGGCAGTAAGAATTCGATTGAAAATAAATTAATTCATTAAAGATGAAATTGGGATTTTTAAAAATAGTCCAAAAGGGAGTAAACTATAACTTAGTCTTTAGAATTTGATGGTTCTGGTTATGACTTATGTCTCCTGATTTTTTGTGAGCTCTGTTTTATATTATTTGGCTCATTAAAATGACTAATTGTATTTCTTCTTGTTCTGTTTTTTTCTTTTGGTTAATGATTGTTTAACTTGAGATCAAATGCACAGACGTCTCCTCGTATTACTTGTCTTTAAGACTTCCAACAAACATAAGCATTATGTGAAGATGACATTCTAATTGAAAAACCTTTAAATCTTTTGCTTTGAGTGAGACTTTTATCTCATCAAAAAAGTCGTCATTTCTGCTGCTTCAGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCAAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGGTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGGTATGGCTGCTTACGCTTATACGACTTTTTATTTCACGAATCTGCAGGAGTGTGCATTTGAAATGATTGATAATTTTCTGTTCATTTATAACGAAGAAAAACTTTTTTCGGCTAGCCTAGCTATAGCATAGTATCTTCCATCATTTATCATCTCACTCTCAAATGAAGTGACAGAACAAAAGAATTTACTCTCGTTGATATTATCTTTGTTCCATTTTTTCAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGGTTTGATATTATACATTCTGCTGTTTTATTTGCTTATGAGGAAATTAAAAAACTTTTTAGTGATCTATGAAAATTTTAACACTCTTGGTCTGGATTTCTTATTGGATTAAATTGTGCGGATTGAGTGGGGATGCAGAGTTCTGAACAAACACCTTTTCTTTTGTCAGAATGACTTTGTTGTGAACATTTGTTGATTGGAACGTAGCAGTAATATATATGTATTTATGACTGTGTTTAAACTTATCACAAAGTCTATAGTTGTTAAGAAATATATAAACATCTTCAGGATGGTAGTTTCTTATATATTTTTTCGGGTTTGTGAACATCTCTTATACGCTTTCTGTGTTGCAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGGTATGAAGAATTTTCACTAACATTTATGCGTCCACTTATTTTTTCTGGTTTTGCTGTTTGAGAATGGTGAAAAGAATACGTAATTCATGTCCCTATTTCTGTCCTTTATTTTTATCCATTTCAGTTCCAGAAGTTATAAAAAAAAAATATTTGGTCTTTCTTTCACGTTTATATAATATCCAAACGTAACCAATAAGAGGTTGACCAAATTATCTAAACTTCCCATATCTAGTCTTCGATTGCCTCTTTTCAGCGGTCAGCCAACTTCTAATTTTGTTTCTTCCGTTGGTTTATTTGAGGGCGTTTCATAATCATTTCATTTTTCGTTTTTAGAAATTATGTTTGTTTTGTATATCTCATTAGTAAATTTTTCGCGAGTCTTGAAAACATGTTTGAATTCTTAGCCAAATTTCAAAAATAAAAATAAATTTTTGAAATTGTTTTCAAAATTTGACTTAGATTTTGAAAATGTTTATAAAAAATTATATAACCAAGTAAAGGAAAAGTTTAATTTTTAAAAATAGAAAATAAAAAATGGAATAGTTATCCAGTTTTATTAATTCAATTTTAATTTTATAACGGAGTGTTTGAGGTCCACGCTCCGTACATCATTAATTTCATGACTAGTGATTTGAAGCTGAGTTGCCCACAATTTTGCTTCCAATATATTCCTAAAATTTGTTACTTTTTCTCCGCAAGCTCAAATTTTTTAAACTGCGCATTATCATATTATATCTAGTTTTTCAAGGTGCAAAATGTGTGTGTGTATATATATATATATATTTGTTGTGCACCTAAAAACGCTCTGATGCTTAAGTCGTAGAGGACTATCCCACTATAGTGGGCCTCACACTTTAAGAAAATATTGTTCATGAATACCTTCACTAATGTTCAGGGTCTTGCTCACCCAACTAAGCTTTGAGATTTAATAGAAGTTTTAAAGTATTAAGTTTTTTATTTGAAGGTTTGAACTCAGGACTTTAGATATTATTTCTAAATATACCGAGACTTCATTATCAGCTTTCCGGTACCTTTATGGTTAGTATTAGTAGACACTTATTTCAACTCAAAATCTTGCTCAATCATTATAATTATCTCTTGCATTTATAATACTATCACACAATACCACACAACCAACTAACGATCAATATGTTATTATTATTATTATTTTTTTTTTGTCCATTAAGCCAAGCCAAAAATAAAGCAATCTAATATAGGCAATCAATCTCTACTTTTTCATATTGTGATATTTTCAATTATTGAGCAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCT

mRNA sequence

ATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCAAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGGTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCT

Coding sequence (CDS)

ATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCAAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGGTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCT

Protein sequence

MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLVSQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQSARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKECKRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA

Homology

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BLAST of MS013872 vs. NCBI nr
Match: XP_022143993.1 (proactivator polypeptide-like 1 [Momordica charantia] >XP_022143994.1 proactivator polypeptide-like 1 [Momordica charantia])

HSP 1 Score: 461.5 bits (1186), Expect = 4.4e-126
Identity = 231/233 (99.14%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEAS+DPKICKLCESLV
Sbjct: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQTVEYLADNKTQDEIT ILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS
Sbjct: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC
Sbjct: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA
Sbjct: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233

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BLAST of MS013872 vs. NCBI nr
Match: KAG6595562.1 (WEB family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.9 bits (969), Expect = 6.5e-101
Identity = 183/233 (78.54%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 529 MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 588

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 589 SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 648

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 649 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 708

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 709 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 761

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BLAST of MS013872 vs. NCBI nr
Match: KAG7027542.1 (Proactivator polypeptide-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377.9 bits (969), Expect = 6.5e-101
Identity = 183/233 (78.54%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 34  MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 93

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 94  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 153

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 154 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 213

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 214 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 266

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BLAST of MS013872 vs. NCBI nr
Match: XP_022925048.1 (proactivator polypeptide-like 1 [Cucurbita moschata])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 183/233 (78.54%), Postives = 210/233 (90.13%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+  LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSLLADA 233

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BLAST of MS013872 vs. NCBI nr
Match: XP_022966507.1 (proactivator polypeptide-like 1 [Cucurbita maxima])

HSP 1 Score: 376.3 bits (965), Expect = 1.9e-100
Identity = 181/233 (77.68%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVVGAAW+CDAR LASSD   SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVGAAWNCDARKLASSDHVSSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+C+SLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECVSLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 233

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BLAST of MS013872 vs. ExPASy Swiss-Prot
Match: Q8C1C1 (Proactivator polypeptide-like 1 OS=Mus musculus OX=10090 GN=Psapl1 PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 3.4e-12
Identity = 46/176 (26.14%), Postives = 82/176 (46.59%), Query Frame = 0


Query: 53  CKLCESLVSQTVEYLADNKTQDEITGILRKTCAVL-GLFNEKCISLVDYYAPLFFSEISS 112
           C +C +LV +  ++L  N T+  I+  L + C V+     ++CI+LVD Y+P     +S 
Sbjct: 295 CDVCLNLVQELDKWLVTNSTEALISHTLERVCTVVPEPLVQQCITLVDTYSPELVQLMSK 354

Query: 113 IEPTSFCQSARFCEQ-----------ITVMSLQIQENS----CGFCHQTVSKMVEKLKDP 172
           + P   C++ + C              T  SL + E +    C  C + +    + L   
Sbjct: 355 VTPEKVCETIKLCGSKRRARSISRAVATTPSLPVDEENQGSFCQGCKRLLGMSSQNLDHK 414

Query: 173 NTQIEILETLLNMCNSLGY-RVKECKRLVFEYGPLIVAKSEKILENTDICRAIHAC 212
           +T+ +IL      C  L    V +C R V EY P+++   + ++  TD+C+ + AC
Sbjct: 415 STKRDILNAFKGGCRILPLPYVMQCNRFVAEYEPVLIESLKFMMNPTDLCKKMGAC 470

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BLAST of MS013872 vs. ExPASy Swiss-Prot
Match: P26779 (Prosaposin OS=Bos taurus OX=9913 GN=PSAP PE=1 SV=3)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 47/171 (27.49%), Postives = 82/171 (47.95%), Query Frame = 0


Query: 53  CKLCESLVSQTVEYLADNKTQDEITGILRKTCAVLGL-FNEKCISLVDYYAPLFFS-EIS 112
           C++CE +V +  + + +N+T++EI   L K C+ L     E+C  +VD Y     S  + 
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGSSILSILLD 375

Query: 113 SIEPTSFCQSARFCEQ-----ITVMSLQIQENS-CGFCHQTVSKMVEKLKDPNTQIEILE 172
              P   C     C        TV  +  ++   C  C + V  +   L+  +T+ +IL 
Sbjct: 376 EASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILA 435

Query: 173 TLLNMCNSL--GYRVKECKRLVFEYGPLIVAKSEKILENTDICRAIHACPA 214
            L   C+ L   YR K+C + V EY P+++    ++++ + +C  I ACPA
Sbjct: 436 ALEKGCSFLPDQYR-KQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPA 485

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BLAST of MS013872 vs. ExPASy TrEMBL
Match: A0A6J1CS32 (proactivator polypeptide-like 1 OS=Momordica charantia OX=3673 GN=LOC111013781 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.1e-126
Identity = 231/233 (99.14%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEAS+DPKICKLCESLV
Sbjct: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQTVEYLADNKTQDEIT ILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS
Sbjct: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC
Sbjct: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA
Sbjct: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233

[Close]

BLAST of MS013872 vs. ExPASy TrEMBL
Match: A0A6J1EE54 (proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111432411 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.3e-101
Identity = 183/233 (78.54%), Postives = 210/233 (90.13%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+  LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSLLADA 233

[Close]

BLAST of MS013872 vs. ExPASy TrEMBL
Match: A0A6J1HN59 (proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466139 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.1e-101
Identity = 181/233 (77.68%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFGL+FLLVVGAAW+CDAR LASSD   SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVGAAWNCDARKLASSDHVSSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EITGIL++TCAVLG+F E+C+SLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECVSLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 233

[Close]

BLAST of MS013872 vs. ExPASy TrEMBL
Match: A0A6J1GCQ1 (proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111452990 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.7e-96
Identity = 173/233 (74.25%), Postives = 202/233 (86.70%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFG++FLLVVG AWDCDARNLAS DSELSYL+Q KD EALSEAS  PK+C+LCESLV
Sbjct: 1   MDLRFGIVFLLVVGVAWDCDARNLASFDSELSYLKQGKDVEALSEASSKPKVCELCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLA+N+TQ EI  ILR+TCAV+GLF E+C+SLVD Y PLFFSE SSIEP S CQS
Sbjct: 61  SQAVEYLAENQTQSEIIRILRQTCAVVGLFKEECVSLVDSYVPLFFSEASSIEPASICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
            RFCEQ+TV+S QIQE+SC FCHQT+SK+++KLKDP+TQ+EIL+ LLN+C+SLG R KEC
Sbjct: 121 VRFCEQVTVISSQIQEHSCEFCHQTISKILDKLKDPDTQVEILQALLNICDSLGKRAKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEKILE TDIC+AIHAC  +AGGD   S VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKILEQTDICKAIHACTGIAGGDKAISSVGTVSSLADA 233

[Close]

BLAST of MS013872 vs. ExPASy TrEMBL
Match: A0A6J1IPM0 (proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111478309 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.2e-95
Identity = 172/233 (73.82%), Postives = 202/233 (86.70%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           MDLRFG++F LVVGAAWDCDARNLAS DSELSYL+Q KD EALSEAS  PK+C+LCESLV
Sbjct: 1   MDLRFGIVFFLVVGAAWDCDARNLASFDSELSYLKQGKDVEALSEASSKPKVCELCESLV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLA+N+TQ EI  ILR+TCAV+GLF E+C+SLVD Y PLFFSE SSIEP S CQS
Sbjct: 61  SQAVEYLAENQTQSEIIRILRQTCAVVGLFKEECVSLVDSYVPLFFSEASSIEPASICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
            RFCEQ+TV+S QIQE+SC FCHQT+SK+++KLKDP+TQ+EIL+ LLN+C+SLG R KEC
Sbjct: 121 VRFCEQVTVISSQIQEHSCEFCHQTISKILDKLKDPDTQVEILQALLNICDSLGKRAKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           K+LVFEYGPLI+A SEKILE TDIC+AIH+CP +AG D   S VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKILEQTDICKAIHSCPGIAGDDKAISSVGTVSSLADA 233

[Close]

BLAST of MS013872 vs. TAIR 10
Match: AT3G51730.1 (saposin B domain-containing protein )

HSP 1 Score: 166.0 bits (419), Expect = 3.6e-41
Identity = 84/212 (39.62%), Postives = 125/212 (58.96%), Query Frame = 0


Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLV 60
           M L+ G   LL++G     DAR+    DS +S            + S    +C LCE  V
Sbjct: 1   MGLKAGTFVLLLLGLILVSDARSFV--DSTIS-----------EKVSNKEDVCTLCEEYV 60

Query: 61  SQTVEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           +  + YL  N TQ EI   L   C+ L  ++++CISLVDYY PLFF ++ S +P  FC+ 
Sbjct: 61  TDALSYLEKNVTQAEIIEDLHDRCSQLRGYSQQCISLVDYYVPLFFLQLESFQPHYFCKR 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
              C ++  +  + +++SCG CH+TVS+++ KL+DP+TQ++I+E L+  C SL    K+C
Sbjct: 121 MNLCGKVVALVEEARQDSCGVCHRTVSEILIKLQDPDTQLDIVELLIKGCKSLKNYEKKC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACP 213
           K LVFEYGPLI+  +E+ L   D+C  + ACP
Sbjct: 181 KTLVFEYGPLILVNAEEFLVKNDVCTLLRACP 199

[Close]

BLAST of MS013872 vs. TAIR 10
Match: AT5G01800.1 (saposin B domain-containing protein )

HSP 1 Score: 152.9 bits (385), Expect = 3.1e-37
Identity = 76/231 (32.90%), Postives = 129/231 (55.84%), Query Frame = 0


Query: 4   RFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASKDPKICKLCESLVSQT 63
           RFG++ +L +  +W C A N                 E    A  D ++C+LC+  V+  
Sbjct: 4   RFGVLLVLFL-LSWSCHATNPILL-------------EPFESAHDDNQVCELCDKYVTLV 63

Query: 64  VEYLADNKTQDEITGILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQSARF 123
           ++YL D   Q+E+   L  +C+ +    ++C+S+VD+Y  LFF+++S+I+    C+    
Sbjct: 64  IDYLQDYDNQNELVEALHISCSQIPPLKKQCLSMVDHYTQLFFTQVSTIKSDQICKRLNL 123

Query: 124 CEQIT-VMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKECKR 183
           C+ +T   + Q+ + +C  C +TVS++V KLKDP T+++I+  LL  C SL     +CK+
Sbjct: 124 CQAVTPAFASQVHQGNCEACRETVSEVVTKLKDPETKLKIIRLLLKECKSLNNYQDKCKK 183

Query: 184 LVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           +VFEYGPL++   +K LE  D+C  +H CP   G      +V  + +LAD+
Sbjct: 184 MVFEYGPLMLTDLQKFLEKKDVCTILHVCP---GPATHRDYVPAVESLADS 217

The following BLAST results are available for this feature:

BLAST of MS013872 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143993.1	4.4e-126	99.14	proactivator polypeptide-like 1 [Momordica charantia] >XP_022143994.1 proactivat...	[more]
KAG6595562.1	6.5e-101	78.54	WEB family protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7027542.1	6.5e-101	78.54	Proactivator polypeptide-like 1, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022925048.1	1.1e-100	78.54	proactivator polypeptide-like 1 [Cucurbita moschata]	[more]
XP_022966507.1	1.9e-100	77.68	proactivator polypeptide-like 1 [Cucurbita maxima]	[more]

BLAST of MS013872 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8C1C1	3.4e-12	26.14	Proactivator polypeptide-like 1 OS=Mus musculus OX=10090 GN=Psapl1 PE=1 SV=2	[more]
P26779	1.4e-10	27.49	Prosaposin OS=Bos taurus OX=9913 GN=PSAP PE=1 SV=3	[more]

BLAST of MS013872 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CS32	2.1e-126	99.14	proactivator polypeptide-like 1 OS=Momordica charantia OX=3673 GN=LOC111013781 P...	[more]
A0A6J1EE54	5.3e-101	78.54	proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111432411 PE...	[more]
A0A6J1HN59	9.1e-101	77.68	proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466139 PE=4...	[more]
A0A6J1GCQ1	6.7e-96	74.25	proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111452990 PE...	[more]
A0A6J1IPM0	1.2e-95	73.82	proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111478309 PE=4...	[more]

BLAST of MS013872 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51730.1	3.6e-41	39.62	saposin B domain-containing protein	[more]
AT5G01800.1	3.1e-37	32.90	saposin B domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008373	Saposin	PRINTS	PR01797	SAPOSIN	coord: 53..73 score: 27.21 coord: 189..212 score: 26.79 coord: 136..158 score: 21.74
IPR008139	Saposin B type domain	SMART	SM00741	sapb_4	coord: 51..124 e-value: 1.8E-16 score: 70.7 coord: 137..211 e-value: 7.7E-13 score: 58.7
IPR008139	Saposin B type domain	PROSITE	PS50015	SAP_B	coord: 49..128 score: 14.915199
IPR008139	Saposin B type domain	PROSITE	PS50015	SAP_B	coord: 135..215 score: 12.668799
IPR007856	Saposin-like type B, region 1	PFAM	PF05184	SapB_1	coord: 139..174 e-value: 2.8E-6 score: 27.3 coord: 52..88 e-value: 1.3E-8 score: 34.7
None	No IPR available	GENE3D	1.10.225.10		coord: 134..215 e-value: 1.0E-16 score: 63.1
None	No IPR available	GENE3D	1.10.225.10		coord: 49..125 e-value: 4.7E-15 score: 57.9
None	No IPR available	PANTHER	PTHR11480:SF80	SAPOSIN-RELATED	coord: 1..212
None	No IPR available	PANTHER	PTHR11480	SAPOSIN-RELATED	coord: 1..212
IPR008138	Saposin B type, region 2	PFAM	PF03489	SapB_2	coord: 93..124 e-value: 4.0E-5 score: 23.7 coord: 178..211 e-value: 4.5E-7 score: 30.0
IPR011001	Saposin-like	SUPERFAMILY	47862	Saposin	coord: 137..212
IPR011001	Saposin-like	SUPERFAMILY	47862	Saposin	coord: 51..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS013872.1	MS013872.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0006665	sphingolipid metabolic process
cellular_component	GO:0005764	lysosome