MS013753 (gene) Bitter gourd (TR) v1

Overview
NameMS013753
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein TRANSPORT INHIBITOR RESPONSE 1
Locationscaffold263: 670105 .. 672972 (-)
RNA-Seq ExpressionMS013753
SyntenyMS013753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAGAATGGCGAGTGCGTTTCCGGAGGATGTGATGGAGCATGTGTTCTCGTTTATTCAGTGTCAGAAGGACCGGAACTCGATCTCGATGGTGTGCAAGTCGTGGTACGAGATTGAGAGGTGGTGTAGGAAGCGGATTTTCGTTGGGAACTGCTACGCGGTGAGTCCTGGGATTGTCATACGGCGGTTCCCGGAGGTGAGATCTGTGGAGCTGAAGGGGAAGCCGCACTTCGCGGACTTCAGTTTGGTGCCGGAGGGGTGGGGTGGGTATGTTCAACCGTGGATCGTGGCTATGGCGTCTGCGTACCCTTGGCTCGAGGAGATCAGGTTGAAGCGAATGGTGGTTACGGACGAGAGCTTGGAGCTCATTTCCAAGTCTTTCAAGAATTTTAAGGTCCTTGTGTTGTCGACGTGTGAGGGGTTCAGTACTGATGGACTTGCAGTCATTGCTGCCAACTGCAGGTTTGTTGATTTTGGTTTTGGTTTTGGGTTACGTTTGGTTGATTTGCTTTGTTATGAGGGTTTAAAACTGGCAAAAGGAAGTCGGAGTGAACATTGTGATTGAGAGCTCCAAGTTTTGGGCTTTTACTTCGGTGCCTCCCCCAGGGATTTTCCTCAGTTTAATCCTTTATTTTTATTTTTTTGGTTTGCTCTTGCTCTTGCTCTTGGTTCTCTTTTCTCATATACAGAATTTCTCTTGTCGCTCAGATTTTATTTTGGGTGGGATGAGTAAGAACACCATTGGGCTGAGCTCGAGCTGGGCTCACTGGGAATGACTGGTTCTTCTATGTGCGGTAGCCCTGTAATTACTTGGATGAGTAGATTTAGTCATTTCGCCATTTGATTTTTCTTTCCACTTTTTGGGGTTTTGGAAGGAGGGGCGGGAGGTTGAGGAGGATAAGAAGCTGTTGCTTTCATCCGGCTTTTGGATCCATCGGTCCGGTTTACTTCTCTGTGCTTTTTACCAAATGATACCCTCAATCAGTTTAGGTATACTGTGATGGTTAACGATATTATAAATCTTATCTAGTTATTTGTTGCTTCCTTGACCTTTAATTCTGTTTTGGACTTCATGTTTCTGTTTTCTAGAAGATCTGAGATGAATGAATTGATAATTTGCGCTAAAGTCGTATCACATCTATTATCTTGCCTTATAAGTTAGTTGGTTTAGGAGACTGTTATTTATTAATTGAAATGTTTTGATAATGGTTAGGGTAATTTGAACTACCTTACTTGAATAGAATCTGTTATATAGGTTGTACTACTGTATCCTCGAGTTCAGAGTTAAGAACTGGGAAGTTCTTTTAGTTGCATAGTTCTTGAAAATGGAGAGACGGGTGGCAATTTGGAAGAGGTTTTTCTTTTTCGCTATTCCTATTTATCATTGAAGGGGGGTGGGTTTTGCTTATTTGTAAGTGTTTCTGTCTTTAAACCTTTTGAAGTTTCTATTATGTGTTTTTATATGGTTCGTTGTTGGCTGATTCAGGAATTTGAAAGTCTTGGACTTGCGAGATAGCGATGTTGATGATCTGAATGGACATTGGCTCAGCCATTTTCCTGATTCATATACGTCACTAGTATCCCTCAATATTACTTGTTTGCAGTCTGAGGTGAGTTTTTCTGCATTGGAGCGTCTCGTGGAGAGGTGTCCAAACTTACAGACACTGCGGCTCAATCGTCCTGTTCCCCTTGCTAGGCAAGCTAACCTACTTCGTCACGCACCTCAACTGGTTGAGTTTGGTGCTGGATGTTACACAGCAGACATGAGATCTGATGTTTTTTCCAACTTAATTGGAGCATTTTCAAACTGCACAGAACTGAAGTGTCTATCTGGATTTTGGGACGTCGTCCCTGCATACCTTCCTTCTGTTTATCCGACTTGCTCTCGGCTAACATCCTTAAACTTAAGTGAAGCTATAATTCAAAGTGACGATCTTACCAAGCTTATCAGTCAATGTCATAACTTGCAGAAGCTTTGGGTATCTCTTTTTTCTTACAACTCTTCTCCCATTCTATTATTGCCTTTTAGTCTTTATTATGTTCTTAAATTCAAGATGCATTGTAACTGCAGGTGCTGGATTACATTGAAGACGCTGGCCTTGAAGTACTTGCAGATACATGCAAGGATCTTCGGGAATTAAGAGTATTTCCCTCCGAACCTTATGGACGAGAACCAAATGTCTCTCTCACAGAACAGGGTCTCATCTCTGTTTCTGAAGGTTGCTCAAAGCTACAATCTGTTCTGTACTTCTGTCGTCAAATGACAAATGCTGCTTTAGTGACCATTGCCAGGAATCGCCCGAACATGACTCGATTTCGTCTATGTATTATGGAGCCAGGAACTCCTGACTACCTCACAAATATGCCACTTGATGTAGGCTTTGGTGCCATTGTTGAGTATTGCAAAGATCTGCAGCGCTTTTCTCTTTCTGGTCTTCTTACTGATCGAGTTTTCGAGCTTATAGGAACTTATGGCAAGAAGCTAGAGATGCTATCTGTGGCATTTGCTGGCGATACTGATTTAGGACTTCACCATGTGTTGTCAGGATGCGATAGTCTCCGCAAGCTGGAGATTAGGGACTGTCCTTTTGGCGATAAAGCTCTTTTGGCCAATGCTGCAAAGCTCGAGACAATGCGATCCCTTTGGATGTCTTCTTGCTCGGTGAGTTTTGGAGCATGCAAATTGTTAGCTCAGAAGCTTCCCAAACTTAACGTCGAAGTTATAGATGAGAGGGGTCCCCCAAATACAAGGCCTGAAAGTTGTCCCGTTGAGAGACTTTACATATACCGAACTGTTGCTGGGCATCGGTTTGACATGCCTGGTTTCGTATGGATGTTGGATGGAGATTCTGCTCTAAGGCTTGAT

mRNA sequence

ATGCTGAGAATGGCGAGTGCGTTTCCGGAGGATGTGATGGAGCATGTGTTCTCGTTTATTCAGTGTCAGAAGGACCGGAACTCGATCTCGATGGTGTGCAAGTCGTGGTACGAGATTGAGAGGTGGTGTAGGAAGCGGATTTTCGTTGGGAACTGCTACGCGGTGAGTCCTGGGATTGTCATACGGCGGTTCCCGGAGGTGAGATCTGTGGAGCTGAAGGGGAAGCCGCACTTCGCGGACTTCAGTTTGGTGCCGGAGGGGTGGGGTGGGTATGTTCAACCGTGGATCGTGGCTATGGCGTCTGCGTACCCTTGGCTCGAGGAGATCAGGTTGAAGCGAATGGTGGTTACGGACGAGAGCTTGGAGCTCATTTCCAAGTCTTTCAAGAATTTTAAGGTCCTTGTGTTGTCGACGTGTGAGGGGTTCAGTACTGATGGACTTGCAGTCATTGCTGCCAACTGCAGGAATTTGAAAGTCTTGGACTTGCGAGATAGCGATGTTGATGATCTGAATGGACATTGGCTCAGCCATTTTCCTGATTCATATACGTCACTAGTATCCCTCAATATTACTTGTTTGCAGTCTGAGGTGAGTTTTTCTGCATTGGAGCGTCTCGTGGAGAGGTGTCCAAACTTACAGACACTGCGGCTCAATCGTCCTGTTCCCCTTGCTAGGCAAGCTAACCTACTTCGTCACGCACCTCAACTGGTTGAGTTTGGTGCTGGATGTTACACAGCAGACATGAGATCTGATGTTTTTTCCAACTTAATTGGAGCATTTTCAAACTGCACAGAACTGAAGTGTCTATCTGGATTTTGGGACGTCGTCCCTGCATACCTTCCTTCTGTTTATCCGACTTGCTCTCGGCTAACATCCTTAAACTTAAGTGAAGCTATAATTCAAAGTGACGATCTTACCAAGCTTATCAGTCAATGTCATAACTTGCAGAAGCTTTGGGTGCTGGATTACATTGAAGACGCTGGCCTTGAAGTACTTGCAGATACATGCAAGGATCTTCGGGAATTAAGAGTATTTCCCTCCGAACCTTATGGACGAGAACCAAATGTCTCTCTCACAGAACAGGGTCTCATCTCTGTTTCTGAAGGTTGCTCAAAGCTACAATCTGTTCTGTACTTCTGTCGTCAAATGACAAATGCTGCTTTAGTGACCATTGCCAGGAATCGCCCGAACATGACTCGATTTCGTCTATGTATTATGGAGCCAGGAACTCCTGACTACCTCACAAATATGCCACTTGATGTAGGCTTTGGTGCCATTGTTGAGTATTGCAAAGATCTGCAGCGCTTTTCTCTTTCTGGTCTTCTTACTGATCGAGTTTTCGAGCTTATAGGAACTTATGGCAAGAAGCTAGAGATGCTATCTGTGGCATTTGCTGGCGATACTGATTTAGGACTTCACCATGTGTTGTCAGGATGCGATAGTCTCCGCAAGCTGGAGATTAGGGACTGTCCTTTTGGCGATAAAGCTCTTTTGGCCAATGCTGCAAAGCTCGAGACAATGCGATCCCTTTGGATGTCTTCTTGCTCGGTGAGTTTTGGAGCATGCAAATTGTTAGCTCAGAAGCTTCCCAAACTTAACGTCGAAGTTATAGATGAGAGGGGTCCCCCAAATACAAGGCCTGAAAGTTGTCCCGTTGAGAGACTTTACATATACCGAACTGTTGCTGGGCATCGGTTTGACATGCCTGGTTTCGTATGGATGTTGGATGGAGATTCTGCTCTAAGGCTTGAT

Coding sequence (CDS)

ATGCTGAGAATGGCGAGTGCGTTTCCGGAGGATGTGATGGAGCATGTGTTCTCGTTTATTCAGTGTCAGAAGGACCGGAACTCGATCTCGATGGTGTGCAAGTCGTGGTACGAGATTGAGAGGTGGTGTAGGAAGCGGATTTTCGTTGGGAACTGCTACGCGGTGAGTCCTGGGATTGTCATACGGCGGTTCCCGGAGGTGAGATCTGTGGAGCTGAAGGGGAAGCCGCACTTCGCGGACTTCAGTTTGGTGCCGGAGGGGTGGGGTGGGTATGTTCAACCGTGGATCGTGGCTATGGCGTCTGCGTACCCTTGGCTCGAGGAGATCAGGTTGAAGCGAATGGTGGTTACGGACGAGAGCTTGGAGCTCATTTCCAAGTCTTTCAAGAATTTTAAGGTCCTTGTGTTGTCGACGTGTGAGGGGTTCAGTACTGATGGACTTGCAGTCATTGCTGCCAACTGCAGGAATTTGAAAGTCTTGGACTTGCGAGATAGCGATGTTGATGATCTGAATGGACATTGGCTCAGCCATTTTCCTGATTCATATACGTCACTAGTATCCCTCAATATTACTTGTTTGCAGTCTGAGGTGAGTTTTTCTGCATTGGAGCGTCTCGTGGAGAGGTGTCCAAACTTACAGACACTGCGGCTCAATCGTCCTGTTCCCCTTGCTAGGCAAGCTAACCTACTTCGTCACGCACCTCAACTGGTTGAGTTTGGTGCTGGATGTTACACAGCAGACATGAGATCTGATGTTTTTTCCAACTTAATTGGAGCATTTTCAAACTGCACAGAACTGAAGTGTCTATCTGGATTTTGGGACGTCGTCCCTGCATACCTTCCTTCTGTTTATCCGACTTGCTCTCGGCTAACATCCTTAAACTTAAGTGAAGCTATAATTCAAAGTGACGATCTTACCAAGCTTATCAGTCAATGTCATAACTTGCAGAAGCTTTGGGTGCTGGATTACATTGAAGACGCTGGCCTTGAAGTACTTGCAGATACATGCAAGGATCTTCGGGAATTAAGAGTATTTCCCTCCGAACCTTATGGACGAGAACCAAATGTCTCTCTCACAGAACAGGGTCTCATCTCTGTTTCTGAAGGTTGCTCAAAGCTACAATCTGTTCTGTACTTCTGTCGTCAAATGACAAATGCTGCTTTAGTGACCATTGCCAGGAATCGCCCGAACATGACTCGATTTCGTCTATGTATTATGGAGCCAGGAACTCCTGACTACCTCACAAATATGCCACTTGATGTAGGCTTTGGTGCCATTGTTGAGTATTGCAAAGATCTGCAGCGCTTTTCTCTTTCTGGTCTTCTTACTGATCGAGTTTTCGAGCTTATAGGAACTTATGGCAAGAAGCTAGAGATGCTATCTGTGGCATTTGCTGGCGATACTGATTTAGGACTTCACCATGTGTTGTCAGGATGCGATAGTCTCCGCAAGCTGGAGATTAGGGACTGTCCTTTTGGCGATAAAGCTCTTTTGGCCAATGCTGCAAAGCTCGAGACAATGCGATCCCTTTGGATGTCTTCTTGCTCGGTGAGTTTTGGAGCATGCAAATTGTTAGCTCAGAAGCTTCCCAAACTTAACGTCGAAGTTATAGATGAGAGGGGTCCCCCAAATACAAGGCCTGAAAGTTGTCCCGTTGAGAGACTTTACATATACCGAACTGTTGCTGGGCATCGGTTTGACATGCCTGGTTTCGTATGGATGTTGGATGGAGATTCTGCTCTAAGGCTTGAT

Protein sequence

MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRLD
Homology
BLAST of MS013753 vs. NCBI nr
Match: XP_008451438.1 (PREDICTED: protein TRANSPORT INHIBITOR RESPONSE 1 [Cucumis melo])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 523/583 (89.71%), Postives = 551/583 (94.51%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS FPEDV+EHVFSFIQC KDRNSISMVCKSWYEIER CRKR+FVGNCYAVSP  V
Sbjct: 1   MLRMASTFPEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERCCRKRVFVGNCYAVSPQTV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFP+VRSVELKGKPHFADFSLVP+GWGGYV PWI+AMASAYP LEEIRLKRMVVTDES
Sbjct: 61  IRRFPDVRSVELKGKPHFADFSLVPDGWGGYVYPWILAMASAYPLLEEIRLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DLNGHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLNGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSLVSLNI CL SEVS SALERLV+RCPNL+TLRLNRPVPL R ANLLR APQLVEFG
Sbjct: 181 TYTSLVSLNIACLGSEVSVSALERLVDRCPNLRTLRLNRPVPLDRHANLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCY AD+RS+VFS+L GAF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYMADLRSEVFSSLTGAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKLISQCHNLQKLWVLD+IED+GLE +ADTCKDLRELRVFPSEPYG+EPNVSLTE
Sbjct: 301 QCDDLTKLISQCHNLQKLWVLDFIEDSGLEAVADTCKDLRELRVFPSEPYGQEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCI+EP TPDYLT+ PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCILEPRTPDYLTDQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAGD+DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGDSDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPNTRPESCPVERLYIYR+VAG R DMPGFVW +DGD+ L+L
Sbjct: 541 GPPNTRPESCPVERLYIYRSVAGRRLDMPGFVWTMDGDAPLKL 583

BLAST of MS013753 vs. NCBI nr
Match: XP_038876726.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 522/583 (89.54%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS FPEDV+EHVFSFIQC KDRNSISMVCKSWYEIERWCRKR+FVGNCYAVSP IV
Sbjct: 1   MLRMASTFPEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERWCRKRVFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFP+VRS+ELKGKPHFADFSLVP+GWGGYV PWI+AMASAYP LEEIRLKRMVVTDES
Sbjct: 61  IRRFPDVRSLELKGKPHFADFSLVPDGWGGYVYPWILAMASAYPLLEEIRLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DLNGHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLNGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSLVSLNI CL SEVS SALERLV+RCPNL+TLRLNRPVPL R ANLL  APQLVEFG
Sbjct: 181 TYTSLVSLNIACLGSEVSVSALERLVDRCPNLRTLRLNRPVPLDRHANLLCRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RS+VFS+L GAF+NCTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYTADLRSEVFSSLTGAFTNCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKL++QC+NLQKLWVLDYIED+GLE +ADTCKDLRELRVFPSE YG EPNVSLTE
Sbjct: 301 QCDDLTKLVTQCYNLQKLWVLDYIEDSGLEAVADTCKDLRELRVFPSELYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDYLT  PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYLTEQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAG++DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGNSDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPNTRPESCPVERLYIYR+V G R DMPGFVW +DGD+ LRL
Sbjct: 541 GPPNTRPESCPVERLYIYRSVTGRRLDMPGFVWTMDGDAPLRL 583

BLAST of MS013753 vs. NCBI nr
Match: XP_023514209.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 526/583 (90.22%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS F EDV+EHVFSFIQC KDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSP IV
Sbjct: 1   MLRMASTFQEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFPEVRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP LEEI+LKRMVVTDES
Sbjct: 61  IRRFPEVRSVELKGKPHFADFSLVPDGWGGYVHPWIVAMASAYPLLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DL+GHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLSGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
            YTSLVSLNI CL SEVS SALERLV+RCPNL+TL LNRPVPL R  NLLR APQLVEFG
Sbjct: 181 KYTSLVSLNIACLASEVSISALERLVDRCPNLRTLWLNRPVPLDRHPNLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RSDVFSNL  AF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYTADLRSDVFSNLTTAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           QSDDLTKLISQCHNLQKLWVLDYIED+GLE LADTCKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QSDDLTKLISQCHNLQKLWVLDYIEDSGLEALADTCKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDYLTN PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYLTNQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVE+CKDLQRFSLSGLLTDR FE IG YGKKLEMLSVAFAGDTDL LHHVLSGCD
Sbjct: 421 VGFGAIVEHCKDLQRFSLSGLLTDRCFEYIGNYGKKLEMLSVAFAGDTDLALHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSC VSFGACKLLAQK PKLNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCLVSFGACKLLAQKFPKLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPN RPESCPVE+LYIYR+VAG R DMPGFVW +DGD++ RL
Sbjct: 541 GPPNIRPESCPVEKLYIYRSVAGCRLDMPGFVWTMDGDASPRL 583

BLAST of MS013753 vs. NCBI nr
Match: KAG7025558.1 (Protein TRANSPORT INHIBITOR RESPONSE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 526/583 (90.22%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS F EDV+EHVFSFIQC KDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSP IV
Sbjct: 1   MLRMASTFQEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFPEVRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP LEEI+LKRMVVTDES
Sbjct: 61  IRRFPEVRSVELKGKPHFADFSLVPDGWGGYVHPWIVAMASAYPLLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DL+GHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLSGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
            YTSLVSLNI CL SEVS SALERLV+RCPNL+TL LNRPVPL R  NLLR APQLVEFG
Sbjct: 181 KYTSLVSLNIACLVSEVSISALERLVDRCPNLRTLWLNRPVPLDRHPNLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RSDVFSNL  AF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYTADLRSDVFSNLTTAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           QSDDLTKLISQCHNLQKLWVLDYIED+GLE LADTCKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QSDDLTKLISQCHNLQKLWVLDYIEDSGLEALADTCKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDYLTN PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYLTNQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVE+CKDLQRFSLSGLLTDR FE IG YGKKLEMLSVAFAGDTDL LHHVLSGCD
Sbjct: 421 VGFGAIVEHCKDLQRFSLSGLLTDRCFEYIGNYGKKLEMLSVAFAGDTDLALHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSC VSFGACKLLAQK PKLNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCLVSFGACKLLAQKFPKLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPN RPESCPVE+LYIYR+VAG R DMPGFVW +DGD++ RL
Sbjct: 541 GPPNIRPESCPVEKLYIYRSVAGCRLDMPGFVWTMDGDASPRL 583

BLAST of MS013753 vs. NCBI nr
Match: XP_023548515.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Cucurbita pepo subsp. pepo] >KAG6575863.1 Protein TRANSPORT INHIBITOR RESPONSE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 522/583 (89.54%), Postives = 550/583 (94.34%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           ML+M SAFPEDV+EHVFSFIQC KDRNSISMVCKSWYEIER CRKR+FVGNCYAVSP IV
Sbjct: 1   MLKMVSAFPEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERRCRKRVFVGNCYAVSPRIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
            RRFP+VRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP+LEEI+LKRMVVTDES
Sbjct: 61  TRRFPKVRSVELKGKPHFADFSLVPDGWGGYVYPWIVAMASAYPFLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNL+VLDLR+SDV+DLNGHWLSHFP+
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLEVLDLRESDVEDLNGHWLSHFPE 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSLVSLN  CL SEVS SALERLV+RCPNLQTLRLNRPVPL R A+LLR APQLVEFG
Sbjct: 181 TYTSLVSLNFACLGSEVSISALERLVDRCPNLQTLRLNRPVPLDRHASLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RS+VFSNL GAF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS AII
Sbjct: 241 AGCYTADLRSEVFSNLTGAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYAII 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKLI+QCHNLQKLWVLDYIED+GLE LA  CKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QCDDLTKLITQCHNLQKLWVLDYIEDSGLEALAGICKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDY+TN PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYITNQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAG++DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGNSDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPNTRPESCPVERLYIYR+VAG R DMPGFVW +DGD+ LRL
Sbjct: 541 GPPNTRPESCPVERLYIYRSVAGRRSDMPGFVWTIDGDAPLRL 583

BLAST of MS013753 vs. ExPASy Swiss-Prot
Match: Q570C0 (Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 PE=1 SV=2)

HSP 1 Score: 981.9 bits (2537), Expect = 3.2e-285
Identity = 464/580 (80.00%), Postives = 522/580 (90.00%), Query Frame = 0

Query: 3   RMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIR 62
           R+A +FPE+V+EHVFSFIQ  KDRNS+S+VCKSWYEIERWCR+++F+GNCYAVSP  VIR
Sbjct: 4   RIALSFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIR 63

Query: 63  RFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLE 122
           RFP+VRSVELKGKPHFADF+LVP+GWGGYV PWI AM+S+Y WLEEIRLKRMVVTD+ LE
Sbjct: 64  RFPKVRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLE 123

Query: 123 LISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSY 182
           LI+KSFKNFKVLVLS+CEGFSTDGLA IAA CRNLK LDLR+SDVDD++GHWLSHFPD+Y
Sbjct: 124 LIAKSFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTY 183

Query: 183 TSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAG 242
           TSLVSLNI+CL SEVSFSALERLV RCPNL++L+LNR VPL + A LL+ APQL E G G
Sbjct: 184 TSLVSLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTG 243

Query: 243 CYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQS 302
            YTA++R DV+S L  A S C EL+CLSGFWD VPAYLP+VY  CSRLT+LNLS A +QS
Sbjct: 244 GYTAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQS 303

Query: 303 DDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQG 362
            DL KL+ QC  LQ+LWVLDYIEDAGLEVLA TCKDLRELRVFPSEP+  EPNV+LTEQG
Sbjct: 304 YDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQG 363

Query: 363 LISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVG 422
           L+SVS GC KL+SVLYFCRQMTNAAL+TIARNRPNMTRFRLCI+EP  PDYLT  PLD+G
Sbjct: 364 LVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIG 423

Query: 423 FGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSL 482
           FGAIVE+CKDL+R SLSGLLTD+VFE IGTY KK+EMLSVAFAGD+DLG+HHVLSGCDSL
Sbjct: 424 FGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSL 483

Query: 483 RKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGP 542
           RKLEIRDCPFGDKALLANA+KLETMRSLWMSSCSVSFGACKLL QK+PKLNVEVIDERG 
Sbjct: 484 RKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGA 543

Query: 543 PNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALR 583
           P++RPESCPVER++IYRTVAG RFDMPGFVW +D DS +R
Sbjct: 544 PDSRPESCPVERVFIYRTVAGPRFDMPGFVWNMDQDSTMR 583

BLAST of MS013753 vs. ExPASy Swiss-Prot
Match: Q9ZR12 (GRR1-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=GRH1 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.2e-223
Identity = 376/572 (65.73%), Postives = 451/572 (78.85%), Query Frame = 0

Query: 4   MASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRR 63
           M   FP  V+EH+ SFI   +DRNS+S+VCKSW+E ER  RKR+FVGNCYAVSP  V RR
Sbjct: 1   MGLRFPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRR 60

Query: 64  FPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLEL 123
           FPE+RS+ LKGKPHFAD++LVP+GWGGY  PWI AMA+    LEEIR+KRMVVTDE LE 
Sbjct: 61  FPEMRSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEK 120

Query: 124 ISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYT 183
           I+ SFK+FKVLVL++CEGFSTDG+A IAA CRNL+VL+LR+  V+DL G WLS+FP+S T
Sbjct: 121 IAASFKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESST 180

Query: 184 SLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGC 243
           SLVSL+ +CL SEV  S LERLV R PNL++L+LN  V L    +LLR APQL E G G 
Sbjct: 181 SLVSLDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGS 240

Query: 244 YTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQSD 303
           + A ++ + FS L  AFSNC +L+ LSG WDV+P YLP++Y  C  LTSLNLS A ++  
Sbjct: 241 FAAQLKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMP 300

Query: 304 DLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGL 363
           DL +L+ +C  LQKLWV+D IED GLE +A  CK+LRELRVFPSEP     N+ LTEQGL
Sbjct: 301 DLVELLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGL 360

Query: 364 ISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGF 423
           + VS+GC KL+SVLYFC Q TNAAL TIAR RPN+  FRLC++EP  PDY TN PLD GF
Sbjct: 361 VFVSKGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGF 420

Query: 424 GAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLR 483
            AI E C+DL+R S+SGLL+D+ F+ IG + KK+ MLS+AFAGD+DL LHH+LSGC+SL+
Sbjct: 421 KAIAEGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLK 480

Query: 484 KLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGPP 543
           KLEIRDCPFGD ALL +AAKLETMRSLWMSSC VSFGACKLL+QK+P+LNVEVIDE  PP
Sbjct: 481 KLEIRDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEH-PP 540

Query: 544 NTRPESCPVERLYIYRTVAGHRFDMPGFVWML 576
            +RPES PVER+YIYRTVAG R D P FVW +
Sbjct: 541 ESRPESSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of MS013753 vs. ExPASy Swiss-Prot
Match: Q0DKP3 (Transport inhibitor response 1-like protein Os05g0150500 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150500 PE=2 SV=2)

HSP 1 Score: 700.3 bits (1806), Expect = 1.9e-200
Identity = 332/569 (58.35%), Postives = 427/569 (75.04%), Query Frame = 0

Query: 7   AFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPE 66
           + P++V EH FSF+    DR + +  C SW   ER  R+R+ V NCYA +P   + RFP 
Sbjct: 19  SLPDEVWEHAFSFLPAAADRGAAAGACSSWLRAERRSRRRLAVANCYAAAPRDAVERFPS 78

Query: 67  VRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISK 126
           VR+ E+KGKPHFADF LVP  WG    PWI A A  +P LEE+  KRMVVTDE LE+I+ 
Sbjct: 79  VRAAEVKGKPHFADFGLVPPAWGAAAAPWIAAAADGWPLLEELSFKRMVVTDECLEMIAA 138

Query: 127 SFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLV 186
           SF+NF+VL L +C+GFST GLA IAA CR+L+ LDL++++++D + HWLS FP+S+TSLV
Sbjct: 139 SFRNFQVLRLVSCDGFSTAGLAAIAAGCRHLRELDLQENEIEDCSIHWLSLFPESFTSLV 198

Query: 187 SLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTA 246
           +LN +CL+ EV+ + LERLV RC NL+TL+LN  +PL + A+LL  APQLVE G G ++A
Sbjct: 199 TLNFSCLEGEVNITVLERLVTRCHNLKTLKLNNAIPLDKLASLLHKAPQLVELGTGKFSA 258

Query: 247 DMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQSDDLT 306
           D  SD+F+ L  AF  C  L+ LSG WD VP YLP+ Y  C  LTSLNLS A ++  +L 
Sbjct: 259 DYHSDLFAKLEAAFGGCKSLRRLSGAWDAVPDYLPAFYCVCEGLTSLNLSYATVRGPELI 318

Query: 307 KLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISV 366
           K IS+C NLQ+LWV+D IED GL V+A +C  L+ELRVFPS+P+G      LTE+GL+ V
Sbjct: 319 KFISRCRNLQQLWVMDLIEDHGLAVVASSCNKLQELRVFPSDPFGAG---FLTERGLVDV 378

Query: 367 SEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAI 426
           S  C  L+SVLYFCR+MTN AL+TIA+NRPN T FRLCI+EP TPDY+T  PLD GF AI
Sbjct: 379 SASCPMLESVLYFCRRMTNEALITIAKNRPNFTCFRLCILEPHTPDYITREPLDAGFSAI 438

Query: 427 VEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLE 486
           VE C+ L+R S+SGLLTD VF+ IG +  +LEMLS+AFAG++DLGLH++LSGC SL+KLE
Sbjct: 439 VESCRGLRRLSISGLLTDLVFKSIGAHADRLEMLSIAFAGNSDLGLHYILSGCKSLKKLE 498

Query: 487 IRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERG---PP 546
           IRDCPFGDK LLANAAKLETMRSLWMSSC ++ GAC+ LA+K+P+L+VE++++ G   P 
Sbjct: 499 IRDCPFGDKPLLANAAKLETMRSLWMSSCLLTLGACRQLARKMPRLSVEIMNDPGRSCPL 558

Query: 547 NTRPESCPVERLYIYRTVAGHRFDMPGFV 573
           ++ P+  PVE+LY+YRT+AG R D P  V
Sbjct: 559 DSLPDETPVEKLYVYRTIAGPRSDTPACV 584

BLAST of MS013753 vs. ExPASy Swiss-Prot
Match: Q9LW29 (Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.3e-198
Identity = 337/573 (58.81%), Postives = 430/573 (75.04%), Query Frame = 0

Query: 8   FPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEV 67
           FP++V+EHVF F+   KDRN+IS+VCKSWY+IER+ R+++F+GNCYA++P  ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 68  RSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKS 127
           +S+ LKGKPHFADF+LVP  WGG+V PWI A+A +   LEE+RLKRMVVTDESLEL+S+S
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 128 FKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVS 187
           F NFK LVL +CEGF+TDGLA IAANCR+L+ LDL+++++DD  G WLS FPD+ T+LV+
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 188 LNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTAD 247
           LN  CL+ E +  ALERLV R PNL++L+LNR VPL   A L+  APQ+V+ G G Y  D
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 248 MRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAI-IQSDDLT 307
             S+ +  L+     CT L+ LSGF +  P  L + +P C  LTSLNLS A  I    L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 308 KLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISV 367
           KLI  C  LQ+LW+LD I D GLEV+A TCK+L+ELRVFPS+  G   N ++TE+GL+++
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLG-GGNTAVTEEGLVAI 363

Query: 368 SEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAI 427
           S GC KL S+LYFC+QMTNAALVT+A+N PN  RFRLCI+EP  PD++T+ PLD GFGAI
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 428 VEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLE 487
           V+ CK L+R SLSGLLTD+VF  IG Y  +LEMLS+AFAGDTD G+ +VL+GC  ++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 488 IRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDE----RGP 547
           IRD PFGD ALLA+ +K ETMRSLWMSSC V+   CK LA+K P LNVE+I+E    R  
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 548 PNTRPESCPVERLYIYRTVAGHRFDMPGFVWML 576
            N       V++LY+YRTV G R D P FVW+L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of MS013753 vs. ExPASy Swiss-Prot
Match: Q7XVM8 (Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0395600 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.6e-196
Identity = 336/573 (58.64%), Postives = 427/573 (74.52%), Query Frame = 0

Query: 8   FPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEV 67
           FPE+V+EH+FSF+  Q+DRN++S+VCK WYEIER  R+ +FVGNCYAV  G V  RFP V
Sbjct: 4   FPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNV 63

Query: 68  RSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKS 127
           R++ +KGKPHFADF+LVP  WGGY  PWI A A     LEE+R+KRMVV+DESLEL+++S
Sbjct: 64  RALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARS 123

Query: 128 FKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVS 187
           F  F+ LVL +CEGFSTDGLA +A++C+ L+ LDL++++V+D    WLS FPDS TSLVS
Sbjct: 124 FPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVS 183

Query: 188 LNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTAD 247
           LN  C++ EV+  +LERLV R PNL++LRLNR V +   A +L   P L + G G  T D
Sbjct: 184 LNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDD 243

Query: 248 MRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEA-IIQSDDLT 307
            +++ +  L  A   C  L+ LSGFWD  P  L  +YP C++LT LNLS A  + + DLT
Sbjct: 244 FQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLT 303

Query: 308 KLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISV 367
           K+IS+C  LQ+LWVLD I D GL+V+A +CKDL+ELRVFPS+ Y    + ++TE+GL++V
Sbjct: 304 KMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYS-AVTEEGLVAV 363

Query: 368 SEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAI 427
           S GC KL S+LYFC QMTNAALVT+A+N PN TRFRLCI+EPG PD +T+ PLD GFGAI
Sbjct: 364 SLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAI 423

Query: 428 VEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLE 487
           V  CK LQR S+SGLLTD+VF  IG Y K+LEMLS+AFAGD+D G+ HV++GC +LRKLE
Sbjct: 424 VRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLE 483

Query: 488 IRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGPPNTR 547
           IRD PFGD ALL N A+ ETMRSLWMSSC+V+   C++LA K+P LNVEVI+ER   N  
Sbjct: 484 IRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEM 543

Query: 548 PES----CPVERLYIYRTVAGHRFDMPGFVWML 576
            E+      VE+LY+YRT AG R D P FV +L
Sbjct: 544 EENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL 575

BLAST of MS013753 vs. ExPASy TrEMBL
Match: A0A1S3BRJ6 (protein TRANSPORT INHIBITOR RESPONSE 1 OS=Cucumis melo OX=3656 GN=LOC103492732 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 523/583 (89.71%), Postives = 551/583 (94.51%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS FPEDV+EHVFSFIQC KDRNSISMVCKSWYEIER CRKR+FVGNCYAVSP  V
Sbjct: 1   MLRMASTFPEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERCCRKRVFVGNCYAVSPQTV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFP+VRSVELKGKPHFADFSLVP+GWGGYV PWI+AMASAYP LEEIRLKRMVVTDES
Sbjct: 61  IRRFPDVRSVELKGKPHFADFSLVPDGWGGYVYPWILAMASAYPLLEEIRLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DLNGHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLNGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSLVSLNI CL SEVS SALERLV+RCPNL+TLRLNRPVPL R ANLLR APQLVEFG
Sbjct: 181 TYTSLVSLNIACLGSEVSVSALERLVDRCPNLRTLRLNRPVPLDRHANLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCY AD+RS+VFS+L GAF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYMADLRSEVFSSLTGAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKLISQCHNLQKLWVLD+IED+GLE +ADTCKDLRELRVFPSEPYG+EPNVSLTE
Sbjct: 301 QCDDLTKLISQCHNLQKLWVLDFIEDSGLEAVADTCKDLRELRVFPSEPYGQEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCI+EP TPDYLT+ PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCILEPRTPDYLTDQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAGD+DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGDSDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPNTRPESCPVERLYIYR+VAG R DMPGFVW +DGD+ L+L
Sbjct: 541 GPPNTRPESCPVERLYIYRSVAGRRLDMPGFVWTMDGDAPLKL 583

BLAST of MS013753 vs. ExPASy TrEMBL
Match: A0A6J1H7V2 (protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460882 PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 525/583 (90.05%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS F EDV+EHVFSFIQC KDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSP IV
Sbjct: 1   MLRMASTFQEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFPEVRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP LEEI+LKRMVVTDES
Sbjct: 61  IRRFPEVRSVELKGKPHFADFSLVPDGWGGYVHPWIVAMASAYPLLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNLKVLDLR+SDV+DL+GHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLKVLDLRESDVEDLSGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
            YTSLVSLNI CL SEVS SALERLV+RCPNL+TL LNRPVPL R  NLLR APQLVEFG
Sbjct: 181 KYTSLVSLNIACLVSEVSISALERLVDRCPNLRTLWLNRPVPLDRHPNLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RSDVFSNL  AF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYTADLRSDVFSNLTTAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           QSDDLTKLISQCHNLQKLWVLDYIED+GLE LADTCKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QSDDLTKLISQCHNLQKLWVLDYIEDSGLEALADTCKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDYLTN PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYLTNQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVE+CKDLQRFSLSGLLTDR FE IG YGKKLEMLSVAFAGDTDL LHHVLSGCD
Sbjct: 421 VGFGAIVEHCKDLQRFSLSGLLTDRCFEYIGNYGKKLEMLSVAFAGDTDLALHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKL+TMRSLWMSSC VSFGACKLLAQK PKLNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLQTMRSLWMSSCLVSFGACKLLAQKFPKLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPN RPESCPVE+LYIYR+VAG R DMPGFVW +DGD++ RL
Sbjct: 541 GPPNIRPESCPVEKLYIYRSVAGCRLDMPGFVWTMDGDASPRL 583

BLAST of MS013753 vs. ExPASy TrEMBL
Match: A0A6J1GQR4 (protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456212 PE=4 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 520/583 (89.19%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           ML+M SAFPEDV+EHVFSFIQC KDRNSISMVCKSWYEIER CRKR+FVGNCYAVSP IV
Sbjct: 1   MLKMVSAFPEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERRCRKRVFVGNCYAVSPRIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
            RRFP+VRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP+LEEI+LKRMVVTDES
Sbjct: 61  TRRFPKVRSVELKGKPHFADFSLVPDGWGGYVYPWIVAMASAYPFLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNL+VLDLR+SDV+DLNGHWLSHFP+
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLEVLDLRESDVEDLNGHWLSHFPE 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSL+SLN  CL SEVS SALERLV+RCPNLQTLRLNRPVPL R A+LLR APQLVEFG
Sbjct: 181 TYTSLISLNFACLGSEVSISALERLVDRCPNLQTLRLNRPVPLDRHASLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RS+VFSNL GAF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS AII
Sbjct: 241 AGCYTADLRSEVFSNLTGAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYAII 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKLI+QCHNLQKLWVLDYIED+GLE LA  CKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QCDDLTKLITQCHNLQKLWVLDYIEDSGLEALAGICKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDY+TN  LD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYVTNQSLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAG++DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGNSDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           GPPNTRPESCPVERLYIYR+VAG R DMPGFVW +DGD+ LRL
Sbjct: 541 GPPNTRPESCPVERLYIYRSVAGRRSDMPGFVWTIDGDAPLRL 583

BLAST of MS013753 vs. ExPASy TrEMBL
Match: A0A6J1KSA8 (protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497784 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 524/583 (89.88%), Postives = 544/583 (93.31%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS F EDV+EHVFSFIQC KDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSP IV
Sbjct: 1   MLRMASTFQEDVLEHVFSFIQCHKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFPEVRSVELKGKPHFADFSLVP+GWGGYV PWIVAMASAYP LEEI+LKRMVVTDES
Sbjct: 61  IRRFPEVRSVELKGKPHFADFSLVPDGWGGYVHPWIVAMASAYPLLEEIKLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCRNL+VLDLR+SDV+DL+GHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRNLRVLDLRESDVEDLSGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
            YTSLVSLNI CL SEVS SALERLV+RCPNL+TL LNRPVPL R  NLLR APQLVEFG
Sbjct: 181 KYTSLVSLNIACLVSEVSISALERLVDRCPNLRTLWLNRPVPLDRHPNLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RSDVFSNL  AF++CTELK LSGFWDVVPAYLPSVYPTCSRLTSLNLS A I
Sbjct: 241 AGCYTADLRSDVFSNLTTAFTSCTELKSLSGFWDVVPAYLPSVYPTCSRLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           QSDDLTKLISQCHNLQKLWVLDYIED+GLE LADTCKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QSDDLTKLISQCHNLQKLWVLDYIEDSGLEALADTCKDLRELRVFPSEPYGPEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEP TPDYLTN PLD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPRTPDYLTNQPLD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVE+CKDLQRFSLSGLLTDR FE IG YGKKLEMLSVAFAGDTDL LHHVLSGCD
Sbjct: 421 VGFGAIVEHCKDLQRFSLSGLLTDRCFEYIGNYGKKLEMLSVAFAGDTDLALHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSC VSFGACKLLAQK PKLNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCLVSFGACKLLAQKFPKLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
           G PN RPESCPVE+LYIYR+VAG R DMPGFVW +DGD++ RL
Sbjct: 541 GHPNIRPESCPVEKLYIYRSVAGCRLDMPGFVWTMDGDASPRL 583

BLAST of MS013753 vs. ExPASy TrEMBL
Match: M1GCN5 (Transport inhibitor response 1 OS=Cucumis sativus OX=3659 GN=Csa_7G393970 PE=2 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 518/583 (88.85%), Postives = 549/583 (94.17%), Query Frame = 0

Query: 1   MLRMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIV 60
           MLRMAS FPEDV+EHVFSFIQC +DRNSISMVCKSWYEIERWCRKR+FVGNCYAVSP IV
Sbjct: 1   MLRMASTFPEDVLEHVFSFIQCHQDRNSISMVCKSWYEIERWCRKRVFVGNCYAVSPQIV 60

Query: 61  IRRFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDES 120
           IRRFP+VRSVELKGKPHFADFSLVP+GWGGYV PWI+AMASAYP LEEIRLKRMVVTDES
Sbjct: 61  IRRFPDVRSVELKGKPHFADFSLVPDGWGGYVYPWILAMASAYPLLEEIRLKRMVVTDES 120

Query: 121 LELISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPD 180
           LELISKSFKNFKVLVL TCEGFSTDGLA IAANCR+LKVLDLR+SDV+DLNGHWLSHFPD
Sbjct: 121 LELISKSFKNFKVLVLMTCEGFSTDGLAAIAANCRHLKVLDLRESDVEDLNGHWLSHFPD 180

Query: 181 SYTSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFG 240
           +YTSLVSLNI CL SEVS SALERLV+RCPNL+TLRLNRPVPL R ANLLR APQLVEFG
Sbjct: 181 TYTSLVSLNIACLVSEVSVSALERLVDRCPNLRTLRLNRPVPLDRHANLLRRAPQLVEFG 240

Query: 241 AGCYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAII 300
           AGCYTAD+RS+VFS+L GAF++CTELK LSGFWDVVPAYLPSVYPTCS+LTSLNLS A I
Sbjct: 241 AGCYTADLRSEVFSSLTGAFTSCTELKSLSGFWDVVPAYLPSVYPTCSQLTSLNLSYATI 300

Query: 301 QSDDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTE 360
           Q DDLTKL+SQCHNLQKLWVLD+IED+GLE +A TCKDLRELRVFPSEPYG EPNVSLTE
Sbjct: 301 QCDDLTKLVSQCHNLQKLWVLDFIEDSGLEAVAVTCKDLRELRVFPSEPYGLEPNVSLTE 360

Query: 361 QGLISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLD 420
           QGL+SVSEGC KLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCI+EP TPDY+T   LD
Sbjct: 361 QGLVSVSEGCPKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCILEPWTPDYVTGQALD 420

Query: 421 VGFGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCD 480
           VGFGAIVEYCKDLQRFSLSGLLTDR FE IGTYGKKLEMLSVAFAG++DLGLHHVLSGCD
Sbjct: 421 VGFGAIVEYCKDLQRFSLSGLLTDRCFEYIGTYGKKLEMLSVAFAGESDLGLHHVLSGCD 480

Query: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDER 540
           SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQK P+LNVEVIDER
Sbjct: 481 SLRKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKFPQLNVEVIDER 540

Query: 541 GPPNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALRL 584
            PPNTRPESCPVERLYIYR+VAG R DMPGFVW +DGD+ LRL
Sbjct: 541 DPPNTRPESCPVERLYIYRSVAGRRLDMPGFVWTMDGDAPLRL 583

BLAST of MS013753 vs. TAIR 10
Match: AT3G62980.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 981.9 bits (2537), Expect = 2.3e-286
Identity = 464/580 (80.00%), Postives = 522/580 (90.00%), Query Frame = 0

Query: 3   RMASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIR 62
           R+A +FPE+V+EHVFSFIQ  KDRNS+S+VCKSWYEIERWCR+++F+GNCYAVSP  VIR
Sbjct: 4   RIALSFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIR 63

Query: 63  RFPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLE 122
           RFP+VRSVELKGKPHFADF+LVP+GWGGYV PWI AM+S+Y WLEEIRLKRMVVTD+ LE
Sbjct: 64  RFPKVRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLE 123

Query: 123 LISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSY 182
           LI+KSFKNFKVLVLS+CEGFSTDGLA IAA CRNLK LDLR+SDVDD++GHWLSHFPD+Y
Sbjct: 124 LIAKSFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTY 183

Query: 183 TSLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAG 242
           TSLVSLNI+CL SEVSFSALERLV RCPNL++L+LNR VPL + A LL+ APQL E G G
Sbjct: 184 TSLVSLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTG 243

Query: 243 CYTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQS 302
            YTA++R DV+S L  A S C EL+CLSGFWD VPAYLP+VY  CSRLT+LNLS A +QS
Sbjct: 244 GYTAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQS 303

Query: 303 DDLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQG 362
            DL KL+ QC  LQ+LWVLDYIEDAGLEVLA TCKDLRELRVFPSEP+  EPNV+LTEQG
Sbjct: 304 YDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQG 363

Query: 363 LISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVG 422
           L+SVS GC KL+SVLYFCRQMTNAAL+TIARNRPNMTRFRLCI+EP  PDYLT  PLD+G
Sbjct: 364 LVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIG 423

Query: 423 FGAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSL 482
           FGAIVE+CKDL+R SLSGLLTD+VFE IGTY KK+EMLSVAFAGD+DLG+HHVLSGCDSL
Sbjct: 424 FGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSL 483

Query: 483 RKLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGP 542
           RKLEIRDCPFGDKALLANA+KLETMRSLWMSSCSVSFGACKLL QK+PKLNVEVIDERG 
Sbjct: 484 RKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGA 543

Query: 543 PNTRPESCPVERLYIYRTVAGHRFDMPGFVWMLDGDSALR 583
           P++RPESCPVER++IYRTVAG RFDMPGFVW +D DS +R
Sbjct: 544 PDSRPESCPVERVFIYRTVAGPRFDMPGFVWNMDQDSTMR 583

BLAST of MS013753 vs. TAIR 10
Match: AT4G03190.1 (GRR1-like protein 1 )

HSP 1 Score: 777.3 bits (2006), Expect = 8.5e-225
Identity = 376/572 (65.73%), Postives = 451/572 (78.85%), Query Frame = 0

Query: 4   MASAFPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRR 63
           M   FP  V+EH+ SFI   +DRNS+S+VCKSW+E ER  RKR+FVGNCYAVSP  V RR
Sbjct: 1   MGLRFPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRR 60

Query: 64  FPEVRSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLEL 123
           FPE+RS+ LKGKPHFAD++LVP+GWGGY  PWI AMA+    LEEIR+KRMVVTDE LE 
Sbjct: 61  FPEMRSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEK 120

Query: 124 ISKSFKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYT 183
           I+ SFK+FKVLVL++CEGFSTDG+A IAA CRNL+VL+LR+  V+DL G WLS+FP+S T
Sbjct: 121 IAASFKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESST 180

Query: 184 SLVSLNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGC 243
           SLVSL+ +CL SEV  S LERLV R PNL++L+LN  V L    +LLR APQL E G G 
Sbjct: 181 SLVSLDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGS 240

Query: 244 YTADMRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQSD 303
           + A ++ + FS L  AFSNC +L+ LSG WDV+P YLP++Y  C  LTSLNLS A ++  
Sbjct: 241 FAAQLKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMP 300

Query: 304 DLTKLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGL 363
           DL +L+ +C  LQKLWV+D IED GLE +A  CK+LRELRVFPSEP     N+ LTEQGL
Sbjct: 301 DLVELLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGL 360

Query: 364 ISVSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGF 423
           + VS+GC KL+SVLYFC Q TNAAL TIAR RPN+  FRLC++EP  PDY TN PLD GF
Sbjct: 361 VFVSKGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGF 420

Query: 424 GAIVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLR 483
            AI E C+DL+R S+SGLL+D+ F+ IG + KK+ MLS+AFAGD+DL LHH+LSGC+SL+
Sbjct: 421 KAIAEGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLK 480

Query: 484 KLEIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGPP 543
           KLEIRDCPFGD ALL +AAKLETMRSLWMSSC VSFGACKLL+QK+P+LNVEVIDE  PP
Sbjct: 481 KLEIRDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEH-PP 540

Query: 544 NTRPESCPVERLYIYRTVAGHRFDMPGFVWML 576
            +RPES PVER+YIYRTVAG R D P FVW +
Sbjct: 541 ESRPESSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of MS013753 vs. TAIR 10
Match: AT3G26810.1 (auxin signaling F-box 2 )

HSP 1 Score: 694.1 bits (1790), Expect = 9.5e-200
Identity = 337/573 (58.81%), Postives = 430/573 (75.04%), Query Frame = 0

Query: 8   FPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEV 67
           FP++V+EHVF F+   KDRN+IS+VCKSWY+IER+ R+++F+GNCYA++P  ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 68  RSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKS 127
           +S+ LKGKPHFADF+LVP  WGG+V PWI A+A +   LEE+RLKRMVVTDESLEL+S+S
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 128 FKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVS 187
           F NFK LVL +CEGF+TDGLA IAANCR+L+ LDL+++++DD  G WLS FPD+ T+LV+
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 188 LNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTAD 247
           LN  CL+ E +  ALERLV R PNL++L+LNR VPL   A L+  APQ+V+ G G Y  D
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 248 MRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAI-IQSDDLT 307
             S+ +  L+     CT L+ LSGF +  P  L + +P C  LTSLNLS A  I    L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 308 KLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISV 367
           KLI  C  LQ+LW+LD I D GLEV+A TCK+L+ELRVFPS+  G   N ++TE+GL+++
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLG-GGNTAVTEEGLVAI 363

Query: 368 SEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAI 427
           S GC KL S+LYFC+QMTNAALVT+A+N PN  RFRLCI+EP  PD++T+ PLD GFGAI
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 428 VEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLE 487
           V+ CK L+R SLSGLLTD+VF  IG Y  +LEMLS+AFAGDTD G+ +VL+GC  ++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 488 IRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDE----RGP 547
           IRD PFGD ALLA+ +K ETMRSLWMSSC V+   CK LA+K P LNVE+I+E    R  
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 548 PNTRPESCPVERLYIYRTVAGHRFDMPGFVWML 576
            N       V++LY+YRTV G R D P FVW+L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of MS013753 vs. TAIR 10
Match: AT1G12820.1 (auxin signaling F-box 3 )

HSP 1 Score: 685.6 bits (1768), Expect = 3.4e-197
Identity = 336/574 (58.54%), Postives = 430/574 (74.91%), Query Frame = 0

Query: 8   FPEDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEV 67
           FP++V+EHVF F+   KDRNSIS+VCKSW++IER+ RK +F+GNCYA++P  +IRRFP +
Sbjct: 4   FPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCL 63

Query: 68  RSVELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKS 127
           +S+ LKGKPHFADF+LVP  WGG+V PWI A+A +   LEE+RLKRMVVTDESL+L+S+S
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRS 123

Query: 128 FKNFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVS 187
           F NFK LVL +CEGF+TDGLA IAANCR+L+ LDL+++++DD  G WL+ FPDS T+L+S
Sbjct: 124 FANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTTLMS 183

Query: 188 LNITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTAD 247
           LN  CL+ E + +ALERLV R PNL++L+LNR VPL   A L+  APQLV+ G G Y  +
Sbjct: 184 LNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENE 243

Query: 248 MRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAI-IQSDDLT 307
              + F+ L+ A    T L+ LSGF +V P  LP+ YP C  L SLNLS A  IQ + L 
Sbjct: 244 PDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGNHLI 303

Query: 308 KLISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREP-NVSLTEQGLIS 367
           KLI  C  LQ+LW+LD I D GL V+A TCK+L+ELRVFPS+ +G E  N S+TE GL++
Sbjct: 304 KLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNNASVTEVGLVA 363

Query: 368 VSEGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGA 427
           +S GC KL S+LYFC+QMTNAAL+ +A+N PN  RFRLCI+EP  PD++T   LD GFGA
Sbjct: 364 ISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSLDEGFGA 423

Query: 428 IVEYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKL 487
           IV+ CK L+R S+SGLLTD+VF  IG Y ++LEMLS+AFAGDTD G+ +VL+GC  +RKL
Sbjct: 424 IVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGCKKMRKL 483

Query: 488 EIRDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERG---- 547
           EIRD PFG+ ALLA+  + ETMRSLWMSSC V+ G CK LAQ  P+LNVE+I+E      
Sbjct: 484 EIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINENENNGM 543

Query: 548 PPNTRPESCPVERLYIYRTVAGHRFDMPGFVWML 576
             N   E   V++LY+YRTV G R D P +V +L
Sbjct: 544 EQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL 577

BLAST of MS013753 vs. TAIR 10
Match: AT5G49980.1 (auxin F-box protein 5 )

HSP 1 Score: 561.6 bits (1446), Expect = 7.4e-160
Identity = 280/568 (49.30%), Postives = 376/568 (66.20%), Query Frame = 0

Query: 10  EDVMEHVFSFIQCQKDRNSISMVCKSWYEIERWCRKRIFVGNCYAVSPGIVIRRFPEVRS 69
           E+V+E+V  F+  + DRN+ S+VCKSW+ +E   R  +F+GNCYA+SP  + +RF  VRS
Sbjct: 56  ENVLENVLQFLDSRCDRNAASLVCKSWWRVEALTRSEVFIGNCYALSPARLTQRFKRVRS 115

Query: 70  VELKGKPHFADFSLVPEGWGGYVQPWIVAMASAYPWLEEIRLKRMVVTDESLELISKSFK 129
           + LKGKP FADF+L+P  WG    PW+  MA AYP LE++ LKRM VTD+ L L++ SF 
Sbjct: 116 LVLKGKPRFADFNLMPPDWGANFAPWVSTMAQAYPCLEKVDLKRMFVTDDDLALLADSFP 175

Query: 130 NFKVLVLSTCEGFSTDGLAVIAANCRNLKVLDLRDSDVDDLNGHWLSHFPDSYTSLVSLN 189
            FK L+L  CEGF T G++++A  CR LKVLDL +S+V D    W+S FP+  T L SL 
Sbjct: 176 GFKELILVCCEGFGTSGISIVANKCRKLKVLDLIESEVTDDEVDWISCFPEDVTCLESLA 235

Query: 190 ITCLQSEVSFSALERLVERCPNLQTLRLNRPVPLARQANLLRHAPQLVEFGAGCYTAD-- 249
             C+++ ++F ALE LV R P L+ LRLNR V L     LL  APQL   G G ++ D  
Sbjct: 236 FDCVEAPINFKALEGLVARSPFLKKLRLNRFVSLVELHRLLLGAPQLTSLGTGSFSHDEE 295

Query: 250 MRSDVFSNLIGAFSNCTELKCLSGFWDVVPAYLPSVYPTCSRLTSLNLSEAIIQSDDLTK 309
            +S+   +   AF  C  + CLSGF +++P YLP+++P C+ LTSLN S A I  D    
Sbjct: 296 PQSEQEPDYAAAFRACKSVVCLSGFRELMPEYLPAIFPVCANLTSLNFSYANISPDMFKP 355

Query: 310 LISQCHNLQKLWVLDYIEDAGLEVLADTCKDLRELRVFPSEPYGREPNVSLTEQGLISVS 369
           +I  CH LQ  W LD I D GL+ +A TCK+LRELR+FP +P   +    ++E GL ++S
Sbjct: 356 IILNCHKLQVFWALDSICDEGLQAVAATCKELRELRIFPFDP-REDSEGPVSELGLQAIS 415

Query: 370 EGCSKLQSVLYFCRQMTNAALVTIARNRPNMTRFRLCIMEPGTPDYLTNMPLDVGFGAIV 429
           EGC KL+S+LYFC++MTNAA++ ++ N P +T FRLCIM    PD++T  P+D GFGAIV
Sbjct: 416 EGCRKLESILYFCQRMTNAAVIAMSENCPELTVFRLCIMGRHRPDHVTGKPMDEGFGAIV 475

Query: 430 EYCKDLQRFSLSGLLTDRVFELIGTYGKKLEMLSVAFAGDTDLGLHHVLSGCDSLRKLEI 489
           + CK L R ++SGLLTD+ F  +G YGK +  LSVAFAGD+D+ L HVL GC  L+KLEI
Sbjct: 476 KNCKKLTRLAVSGLLTDQAFRYMGEYGKLVRTLSVAFAGDSDMALRHVLEGCPRLQKLEI 535

Query: 490 RDCPFGDKALLANAAKLETMRSLWMSSCSVSFGACKLLAQKLPKLNVEVIDERGPPNTRP 549
           RD PFGD AL +   +   MR +WMS+CS+S G CK +A+ +P L VEVI      + R 
Sbjct: 536 RDSPFGDVALRSGMHRYYNMRFVWMSACSLSKGCCKDIARAMPNLVVEVIGSDDDDDNRD 595

Query: 550 ESCPVERLYIYRTVAGHRFDMPGFVWML 576
               VE LY+YR++ G R D P FV +L
Sbjct: 596 Y---VETLYMYRSLDGPRNDAPKFVTIL 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008451438.10.0e+0089.71PREDICTED: protein TRANSPORT INHIBITOR RESPONSE 1 [Cucumis melo][more]
XP_038876726.10.0e+0089.54protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida][more]
XP_023514209.10.0e+0090.22protein TRANSPORT INHIBITOR RESPONSE 1-like [Cucurbita pepo subsp. pepo][more]
KAG7025558.10.0e+0090.22Protein TRANSPORT INHIBITOR RESPONSE 1 [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023548515.10.0e+0089.54protein TRANSPORT INHIBITOR RESPONSE 1-like [Cucurbita pepo subsp. pepo] >KAG657... [more]
Match NameE-valueIdentityDescription
Q570C03.2e-28580.00Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 P... [more]
Q9ZR121.2e-22365.73GRR1-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=GRH1 PE=1 SV=1[more]
Q0DKP31.9e-20058.35Transport inhibitor response 1-like protein Os05g0150500 OS=Oryza sativa subsp. ... [more]
Q9LW291.3e-19858.81Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=... [more]
Q7XVM81.6e-19658.64Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. ... [more]
Match NameE-valueIdentityDescription
A0A1S3BRJ60.0e+0089.71protein TRANSPORT INHIBITOR RESPONSE 1 OS=Cucumis melo OX=3656 GN=LOC103492732 P... [more]
A0A6J1H7V20.0e+0090.05protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1GQR40.0e+0089.19protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KSA80.0e+0089.88protein TRANSPORT INHIBITOR RESPONSE 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
M1GCN50.0e+0088.85Transport inhibitor response 1 OS=Cucumis sativus OX=3659 GN=Csa_7G393970 PE=2 S... [more]
Match NameE-valueIdentityDescription
AT3G62980.12.3e-28680.00F-box/RNI-like superfamily protein [more]
AT4G03190.18.5e-22565.73GRR1-like protein 1 [more]
AT3G26810.19.5e-20058.81auxin signaling F-box 2 [more]
AT1G12820.13.4e-19758.54auxin signaling F-box 3 [more]
AT5G49980.17.4e-16049.30auxin F-box protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 479..503
e-value: 61.0
score: 8.4
coord: 370..394
e-value: 4.7
score: 16.2
coord: 103..127
e-value: 320.0
score: 2.8
IPR001810F-box domainSMARTSM00256fbox_2coord: 8..49
e-value: 9.1E-5
score: 31.8
IPR041101Transport inhibitor response 1 domainPFAMPF18791Transp_inhibitcoord: 65..111
e-value: 3.6E-26
score: 90.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 45..575
e-value: 6.3E-182
score: 607.6
NoneNo IPR availableGENE3D1.20.1280.50coord: 2..44
e-value: 1.3E-19
score: 71.4
NoneNo IPR availablePANTHERPTHR16134:SF66PROTEIN TRANSPORT INHIBITOR RESPONSE 1coord: 6..575
NoneNo IPR availablePANTHERPTHR16134F-BOX/TPR REPEAT PROTEIN POF3coord: 6..575
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 68..532
IPR041567COI1, F-boxPFAMPF18511F-box_5coord: 6..46
e-value: 1.4E-18
score: 66.2
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 6..40

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013753.1MS013753.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding