MS013642 (gene) Bitter gourd (TR) v1

Overview
NameMS013642
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionheavy metal-associated isoprenylated plant protein 3-like
Locationscaffold1457: 65525 .. 66984 (+)
RNA-Seq ExpressionMS013642
SyntenyMS013642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTCAGGCGGTGCAGAAGAAGAAGAAGAATGAGAATGAGAAGAATGGAGATGGCGGAGGAGGCGCGGGGAAGAAGAAGGAAGAGAATCCAGTTACTGTGGTGCTTAAGGTCGATATGCATTGTGAAGGCTGTGCCAACAAAATCATCAAATGTGTTAAAGGATTTGAAGGTATATGTGTATTGTAAATGGCTGGCTATGGCGGATTTTGCTTCGAAATCTCCATTTCTCCATTACTGGAAGTGAATTTACTGTGATTGCCTCGATTTTCCCCATCTGGGTGTTCCTGTTTTTGGTATCGTTTTGAGTCAGTTTTTGCTTACTCTGTTTTCCATTCCACTCTTCAACTGTTTTCTGACTCCAATGTTTAGGAATCGATGAATCCCAAATCGGATTTCCATTTTTTTTTCTGTGTGATTTTGCGATTATCTGACGGACACACCATTTCTGGGCCTCTGTTTTTGGGTTTCTGTGTTCTTCAGGTGTACAGACTGTGAAAGCTGAGATTGAGGCCAACAAACTGACTGTGATGGGGAAGAAAATAGACCCATCGCAACTCCGGGAGAAGCTCTTCAACAAGACGAAGAAGAAAGTGGATTTAATTTCTCCACAACCCAAGAAAGAAAAGGACTCGAAACCCAACGACGACGCCCAAAATAACACCAGCAAATCCGACAAAAAAACAGACGAGAAGAAGAAACCCAAAGAGGTAATTATAAAAAGAAAACCCAAAAACTGGTCCCCTCTGAGATTCCTCCGCCGCTTGATTTAACTTCTCTTCTGTTGTTTCCGATTCTTCAGCCTCCGGTGACGACGGCGGTGCTGAAGGTGGCGCTCCACTGCCAGGGATGCATCGAGAAGATTCACAGAGTCACATCCAAGTTCAAAGGTAAGAACTAAGAACCCACATTCGCCGCCGGCATTGCCGCCATTCCGGCGTTGTAAGGACTTCCGTTTTGAACATTTGATTACAGGAGTGCACGAGATGTCAGTGGAGCGGCAGAAGGATTTGGTGACGGTGAAGGGCACAATGGACATTAAGGCTCTGAGCGAGAGCTTAACGGAGAGACTAAAACGTCCCGTCGCCATCATCCCGGCGAAGAAGGAGAAAGAAAAGGACAACAACAACAACAAGAAGAACAACAACGGTGACAATAAAGAAGCCGGCGGAGAGGGCGGAGAGGGCGAGACGAAGGCACCCGAAGGCGGCGGGGGCGGAAAGAAGAAGAAGAAGGGCGGCGGCGGAGGAGGAGGAGGGAACGGCGGGGAAGAGGACGGCGGCGGCGGCGGCGGAGGGAAGATGGAGGGGAACAAAATGGAGTACATGGGAATGACAGGGTACGGGTACGGATTGAATTATGGGTATGGGTACGGGTACGGACCTGGAGGGCTGGTGGGGGAGAATTTGCACGCGCCACAAATATTCAGCGACGAAAATCCAAATGCCTGCTCCATAATG

mRNA sequence

GTTTTTCAGGCGGTGCAGAAGAAGAAGAAGAATGAGAATGAGAAGAATGGAGATGGCGGAGGAGGCGCGGGGAAGAAGAAGGAAGAGAATCCAGTTACTGTGGTGCTTAAGGTCGATATGCATTGTGAAGGCTGTGCCAACAAAATCATCAAATGTGTTAAAGGATTTGAAGGTGTACAGACTGTGAAAGCTGAGATTGAGGCCAACAAACTGACTGTGATGGGGAAGAAAATAGACCCATCGCAACTCCGGGAGAAGCTCTTCAACAAGACGAAGAAGAAAGTGGATTTAATTTCTCCACAACCCAAGAAAGAAAAGGACTCGAAACCCAACGACGACGCCCAAAATAACACCAGCAAATCCGACAAAAAAACAGACGAGAAGAAGAAACCCAAAGAGCCTCCGGTGACGACGGCGGTGCTGAAGGTGGCGCTCCACTGCCAGGGATGCATCGAGAAGATTCACAGAGTCACATCCAAGTTCAAAGGAGTGCACGAGATGTCAGTGGAGCGGCAGAAGGATTTGGTGACGGTGAAGGGCACAATGGACATTAAGGCTCTGAGCGAGAGCTTAACGGAGAGACTAAAACGTCCCGTCGCCATCATCCCGGCGAAGAAGGAGAAAGAAAAGGACAACAACAACAACAAGAAGAACAACAACGGTGACAATAAAGAAGCCGGCGGAGAGGGCGGAGAGGGCGAGACGAAGGCACCCGAAGGCGGCGGGGGCGGAAAGAAGAAGAAGAAGGGCGGCGGCGGAGGAGGAGGAGGGAACGGCGGGGAAGAGGACGGCGGCGGCGGCGGCGGAGGGAAGATGGAGGGGAACAAAATGGAGTACATGGGAATGACAGGGTACGGGTACGGATTGAATTATGGGTATGGGTACGGGTACGGACCTGGAGGGCTGGTGGGGGAGAATTTGCACGCGCCACAAATATTCAGCGACGAAAATCCAAATGCCTGCTCCATAATG

Coding sequence (CDS)

GTTTTTCAGGCGGTGCAGAAGAAGAAGAAGAATGAGAATGAGAAGAATGGAGATGGCGGAGGAGGCGCGGGGAAGAAGAAGGAAGAGAATCCAGTTACTGTGGTGCTTAAGGTCGATATGCATTGTGAAGGCTGTGCCAACAAAATCATCAAATGTGTTAAAGGATTTGAAGGTGTACAGACTGTGAAAGCTGAGATTGAGGCCAACAAACTGACTGTGATGGGGAAGAAAATAGACCCATCGCAACTCCGGGAGAAGCTCTTCAACAAGACGAAGAAGAAAGTGGATTTAATTTCTCCACAACCCAAGAAAGAAAAGGACTCGAAACCCAACGACGACGCCCAAAATAACACCAGCAAATCCGACAAAAAAACAGACGAGAAGAAGAAACCCAAAGAGCCTCCGGTGACGACGGCGGTGCTGAAGGTGGCGCTCCACTGCCAGGGATGCATCGAGAAGATTCACAGAGTCACATCCAAGTTCAAAGGAGTGCACGAGATGTCAGTGGAGCGGCAGAAGGATTTGGTGACGGTGAAGGGCACAATGGACATTAAGGCTCTGAGCGAGAGCTTAACGGAGAGACTAAAACGTCCCGTCGCCATCATCCCGGCGAAGAAGGAGAAAGAAAAGGACAACAACAACAACAAGAAGAACAACAACGGTGACAATAAAGAAGCCGGCGGAGAGGGCGGAGAGGGCGAGACGAAGGCACCCGAAGGCGGCGGGGGCGGAAAGAAGAAGAAGAAGGGCGGCGGCGGAGGAGGAGGAGGGAACGGCGGGGAAGAGGACGGCGGCGGCGGCGGCGGAGGGAAGATGGAGGGGAACAAAATGGAGTACATGGGAATGACAGGGTACGGGTACGGATTGAATTATGGGTATGGGTACGGGTACGGACCTGGAGGGCTGGTGGGGGAGAATTTGCACGCGCCACAAATATTCAGCGACGAAAATCCAAATGCCTGCTCCATAATG

Protein sequence

VFQAVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKPNDDAQNNTSKSDKKTDEKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGYGYGYGPGGLVGENLHAPQIFSDENPNACSIM
Homology
BLAST of MS013642 vs. NCBI nr
Match: XP_008463119.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo] >KAA0051833.1 heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 414.1 bits (1063), Expect = 1.1e-111
Identity = 246/332 (74.10%), Postives = 270/332 (81.33%), Query Frame = 0

Query: 3   QAVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTV 62
           +AVQKKKKN+NEKNGDG GG GKKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQ+V
Sbjct: 3   EAVQKKKKNDNEKNGDGEGG-GKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQSV 62

Query: 63  KAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNN 122
           KAEI+ NKLTVMGKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N
Sbjct: 63  KAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSN 122

Query: 123 TSKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLV 182
            +K DKKTDE KKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGV EMSV+RQKD V
Sbjct: 123 NNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDSV 182

Query: 183 TVKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETK 242
            VKGTMD+KAL  SL+ERLKRPV I+PAKKEKEK+  NNK    G + +     G+G+  
Sbjct: 183 MVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEKEKENNKNEGGGGDDKKDSPTGDGDGN 242

Query: 243 APEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY--- 302
               G GG KKKK GG GGGG G  E+GGGGGGGKMEGNKMEYMGM G GYG  YGY   
Sbjct: 243 GNGNGNGGGKKKKKGGNGGGGEG--EEGGGGGGGKMEGNKMEYMGMGGIGYGYGYGYGLN 302

Query: 303 -GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
            GYGYGPGGLVGENLHAPQ+FSDENPNAC IM
Sbjct: 303 TGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of MS013642 vs. NCBI nr
Match: XP_004147287.1 (heavy metal-associated isoprenylated plant protein 3 [Cucumis sativus] >KGN64800.1 hypothetical protein Csa_013474 [Cucumis sativus])

HSP 1 Score: 412.9 bits (1060), Expect = 2.5e-111
Identity = 249/336 (74.11%), Postives = 273/336 (81.25%), Query Frame = 0

Query: 4   AVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVK 63
           AVQKKKKN+NEKNGDGGGG GKKKEE P T+VLK+DMHCEGCANKI KCVKGFEGVQ+VK
Sbjct: 5   AVQKKKKNDNEKNGDGGGGEGKKKEEIPFTIVLKIDMHCEGCANKITKCVKGFEGVQSVK 64

Query: 64  AEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNT 123
           AEI+ NKLTVMGKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N 
Sbjct: 65  AEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSNN 124

Query: 124 SKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVT 183
           +KSDKKTDE KKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGV EMSV++QKD V 
Sbjct: 125 NKSDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDKQKDSVM 184

Query: 184 VKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNG---DNKEAGGEGGEGE 243
           VKGTMD+KAL  SL+ERLKR V I+PAKKEKEK+ +NNKK   G   + K++    G+G 
Sbjct: 185 VKGTMDVKALIGSLSERLKRTVEIVPAKKEKEKEKDNNKKEGGGGGDEKKDSTTGDGDG- 244

Query: 244 TKAPEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY- 303
                G GGGKKKKKGG GGGG   GEE  GGGGGGKMEGNKMEYMGM G GYG  YGY 
Sbjct: 245 ----NGNGGGKKKKKGGNGGGG--DGEE--GGGGGGKMEGNKMEYMGMGGIGYGYGYGYG 304

Query: 304 -----GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
                GYGYGP G+VGENLHAPQ+FSDENPNAC IM
Sbjct: 305 YGMNTGYGYGPSGIVGENLHAPQLFSDENPNACFIM 331

BLAST of MS013642 vs. NCBI nr
Match: XP_038894057.1 (heavy metal-associated isoprenylated plant protein 3-like [Benincasa hispida])

HSP 1 Score: 411.0 bits (1055), Expect = 9.6e-111
Identity = 251/331 (75.83%), Postives = 272/331 (82.18%), Query Frame = 0

Query: 3   QAVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTV 62
           +AVQKKKKNENE NGDGGGG GKKKEE P TVVLK+DMHCEGCANKI KCVKGFEGVQ+V
Sbjct: 3   EAVQKKKKNENENNGDGGGG-GKKKEEIPFTVVLKIDMHCEGCANKITKCVKGFEGVQSV 62

Query: 63  KAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNN 122
           KAEI+ NKLTV GKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N
Sbjct: 63  KAEIDGNKLTVTGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSN 122

Query: 123 TSKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLV 182
            +KSDKKTDE KKK KEPPVTTAVLKV+LHCQGCIEKI RVT+KFKGV EMSV+RQKDLV
Sbjct: 123 NNKSDKKTDENKKKSKEPPVTTAVLKVSLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDLV 182

Query: 183 TVKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETK 242
           TVKGTMD+KALS SLTERLKRPV I+P K  KEKDNN N+    G +K+     G+G   
Sbjct: 183 TVKGTMDVKALSGSLTERLKRPVEIVPMK--KEKDNNKNEGGGGGGDKKDPAATGDGN-- 242

Query: 243 APEGGGGGKKKKKGGGGGGG---GNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY 302
              G GGGKKKKKGG GGGG   GNGGE+    G GGKMEGNKMEYMGM G GYG  +GY
Sbjct: 243 -GNGNGGGKKKKKGGNGGGGGGDGNGGED----GCGGKMEGNKMEYMGMVGIGYG--HGY 302

Query: 303 GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
           GYGYGPGGLVGENLHAPQ+FSDENPNAC IM
Sbjct: 303 GYGYGPGGLVGENLHAPQLFSDENPNACFIM 321

BLAST of MS013642 vs. NCBI nr
Match: XP_022965017.1 (heavy metal-associated isoprenylated plant protein 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 404.4 bits (1038), Expect = 9.0e-109
Identity = 249/335 (74.33%), Postives = 271/335 (80.90%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAE 65
           +KK KNENEKNGDGGG  GKKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQTVKAE
Sbjct: 3   EKKTKNENEKNGDGGG--GKKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQTVKAE 62

Query: 66  IEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNTSK 125
           IE NKLTV G+KID S+L EKL NKTKKKVDLISPQ KKEKDSKP      D   +N +K
Sbjct: 63  IEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSSNNNK 122

Query: 126 SDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVK 185
           SDKKT+E KKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGV EMS+++QK+LVTVK
Sbjct: 123 SDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKELVTVK 182

Query: 186 GTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNN--NGDNKEAGGEGGEGETKA 245
           GTMD+KALS  LTERLKR V I+P KKEK+KDNNNN   N   G NK+     GEG+  +
Sbjct: 183 GTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAGEGDGNS 242

Query: 246 PEGGGGGKKKKKGGGGGGGGNGGEEDGG-----GGGGGKMEGNKMEYMGM---TGYGYGL 305
            E  GGGKKKKKGG G GGG+GG + G      GGGGGKMEG KMEYMGM    GYGYGL
Sbjct: 243 NE-NGGGKKKKKGGNGNGGGDGGGDGGATGMEEGGGGGKMEGYKMEYMGMGGGIGYGYGL 302

Query: 306 NYGYGYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
           N GYGYGYG GG+VGENLHAPQ+FSDENPNAC IM
Sbjct: 303 N-GYGYGYGAGGVVGENLHAPQLFSDENPNACWIM 333

BLAST of MS013642 vs. NCBI nr
Match: KAG6583560.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019268.1 Heavy metal-associated isoprenylated plant protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 402.1 bits (1032), Expect = 4.4e-108
Identity = 251/345 (72.75%), Postives = 272/345 (78.84%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAE 65
           +KK KNENEKNGDGGG  GKKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQTVKAE
Sbjct: 3   EKKTKNENEKNGDGGG--GKKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQTVKAE 62

Query: 66  IEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNTSK 125
           IE NKLTV G+KID S+L EKL NKTKKKVDLISPQ KKEKDSKP      D   +N +K
Sbjct: 63  IEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSSNNNK 122

Query: 126 SDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVK 185
           SDKKT+E KKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGV EMS+++QK+LVTVK
Sbjct: 123 SDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKELVTVK 182

Query: 186 GTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNN----NGDNKEAGGEGGEGET 245
           GTMD+KALS  LTERLKR V I+P KKEK+KDNNN   NN     G NK+     GEG+ 
Sbjct: 183 GTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNTNNNNKNEGGGGNKKEPPATGEGDG 242

Query: 246 KAPEGGGGGKKKKKGGGGGGGGNGGEEDGG------GGGGGKMEGNKMEYMGM---TGYG 305
            + E GGG KKKK G G GGGG+GG  DGG      GGGGGKMEG KMEYMGM    GYG
Sbjct: 243 NSNENGGGKKKKKGGNGNGGGGSGG--DGGATGMEEGGGGGKMEGYKMEYMGMGGGIGYG 302

Query: 306 YGLN-------YGYGYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
           YGLN        GYGYGYG GG+VGENLHAPQ+FSDENPNACSIM
Sbjct: 303 YGLNGYGMSTGLGYGYGYGAGGVVGENLHAPQLFSDENPNACSIM 343

BLAST of MS013642 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-62
Identity = 168/326 (51.53%), Postives = 211/326 (64.72%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENP-VTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKA 65
           +KK + +N+K     GG  KKK E P +TVVLKVDMHCEGCA++I+KCV+ F+GV+TVK+
Sbjct: 3   EKKNEGDNKKK----GGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKS 62

Query: 66  EIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSK---PNDDAQNNTSKS 125
           E    KLTV G  +DP +LREKL  KTKKKVDL+SPQPKKEK+ +    ND+ +  + + 
Sbjct: 63  ESATGKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEK 122

Query: 126 DKKTDEKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGT 185
            K  +  KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV+ ++++++K+L+TVKGT
Sbjct: 123 KKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGT 182

Query: 186 MDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGG 245
           MD+K L E L+E+LKR V I+P KKEK+K+N              G E GE         
Sbjct: 183 MDVKKLVEILSEKLKRAVEIVPPKKEKDKEN--------------GNENGE--------- 242

Query: 246 GGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEG-NKMEYMGMTGYGYGLNYGYGYGYGPG 305
                KKKGGGG GGG   E+ G  GGG   EG N MEYM            YGYGY PG
Sbjct: 243 -----KKKGGGGDGGGK--EKTGNKGGG---EGVNMMEYM-------AAQPAYGYGYYPG 283

Query: 306 GLVGENL--HAPQIFSDENPNACSIM 325
           G  G  +  HAPQIFSDENPNAC +M
Sbjct: 303 GPYGYPIQAHAPQIFSDENPNACVVM 283

BLAST of MS013642 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.8e-34
Identity = 94/222 (42.34%), Postives = 143/222 (64.41%), Query Frame = 0

Query: 25  KKKEEN---PVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVMGKKIDPS 84
           KKK+ N   PV VVLK+D HC+GC  +I++  +  EGV+TV+A+ ++NKLT++G  +DP 
Sbjct: 19  KKKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPV 78

Query: 85  QLREKLFNKTKKKVDLISPQPKKEKDSKPNDDAQNNTSKSDKKTDEKKKPKEPPVTTAVL 144
           ++ EKL  K+KKKV+LISP+PKK       D  +NN  K++ KT          VTT VL
Sbjct: 79  KIAEKLQKKSKKKVELISPKPKK-------DTKENNEKKANDKTQ-----TVVAVTTVVL 138

Query: 145 KVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGTMDIKALSESLTERLKRPVAI 204
           KV   C GCI++I +  S  KGV+++ ++++K+ VTV GTMDIK+++++L  +LK+ V +
Sbjct: 139 KVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQV 198

Query: 205 IPAKKEKEKDNNNNKKNNNGDNKEAGGEG---GEGETKAPEG 241
           +P KK+K+KD +N + N    +    G G   G G  +  EG
Sbjct: 199 VPEKKKKKKDKDNAEVNTKVGSPCQPGNGCTHGIGPYRFMEG 228

BLAST of MS013642 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 8.3e-33
Identity = 140/355 (39.44%), Postives = 184/355 (51.83%), Query Frame = 0

Query: 20  GGGAGKKKEENPVTVVL-----KVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVM 79
           GGG        PV  V+     KVDMHCEGCA KI + VK F+GV+ V A+   NKL V+
Sbjct: 33  GGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVV 92

Query: 80  GKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKPNDDAQNNTSKSDKKTDEKKKPKEP 139
           G KIDP +L+EKL  KTK+KV L +P PK E         +       +       P  P
Sbjct: 93  G-KIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAP 152

Query: 140 PVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGTMDIKALSESLTER 199
             +   LK+ LHC+GCI+KI ++  K KGV  ++++  KD+VTVKGT+D+K L   LT++
Sbjct: 153 KESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKK 212

Query: 200 LKRPV-AIIPAKKEKEKDNNNNKKNNNGDNK---------EAGGEGGEGETKAPEGGGGG 259
           LKR V  ++PAKK+     N   +    D K         EA  EG +G  K  E G GG
Sbjct: 213 LKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGG 272

Query: 260 KKKKKGGGGG----GGGNGGE--EDGGGGGGGKMEGNKMEYMGMT-----------GYGY 319
           +KKK+GG GG      G+GGE  +DGGG        NKM+Y G +           G+ Y
Sbjct: 273 EKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVY 332

Query: 320 GLNY---------------GYGYGYGPGGLV---GENLHAPQIFSDENPNACSIM 325
           G +Y               G GY Y     V     N++AP +FSDENPN CS+M
Sbjct: 333 GQSYSMTGQNYPVGGQSYPGSGYNYASESYVPYAQPNVNAPGMFSDENPNGCSVM 386

BLAST of MS013642 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.9e-32
Identity = 138/392 (35.20%), Postives = 191/392 (48.72%), Query Frame = 0

Query: 22  GAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVMGKKIDPS 81
           G  K  +    TVV+K+DMHCEGC  KI +  K F+GV+ VK + ++NKLTV+G  +DP 
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIG-NVDPV 73

Query: 82  QLREKLFNKTKKKVDLIS--PQPKKE---------------KDSKPNDDAQNNTSKSDKK 141
           ++R+K+ +K K+ V+L+S    PKKE                + KP +       K  +K
Sbjct: 74  EVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEK 133

Query: 142 TDEKKK---------PKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDL 201
            +EKKK         P  P  +T VLK  LHC+GC  KI R+ +K KGV+ ++++  KDL
Sbjct: 134 KEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDL 193

Query: 202 VTVKGTMDIKALSESLTERLKRPVAIIPAKKE--------KEKDNNNNKKNNNGDNKEAG 261
           V VKG +D+K L+  L E+LKR V ++PAKK+                KK+     K+  
Sbjct: 194 VIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEI 253

Query: 262 GEGGEGETKAPEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGY 321
            + GE   K  +GGG  KK+   GGGGGGG      GGGG GG M+  K EY    GYGY
Sbjct: 254 KDVGE---KKVDGGGEKKKEVAVGGGGGGG------GGGGDGGAMDVKKSEY---NGYGY 313

Query: 322 G-------------------------------------------LNYGYGYGYG----PG 325
                                                       +N GYG GYG    P 
Sbjct: 314 PPQPMYYYPEGQVYGQQHYMMQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPP 373

BLAST of MS013642 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.6e-22
Identity = 114/333 (34.23%), Postives = 160/333 (48.05%), Query Frame = 0

Query: 8   KKKNENEKNGDGGGGAGKKKEENPV------TVVLKVDMHCEGCANKIIKCVKGFEGVQT 67
           KK  E E N D    +     E P        VVLKV MHCEGCA K+ +C+KGFEGV+ 
Sbjct: 43  KKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLKGFEGVED 102

Query: 68  VKAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKPNDDAQNNTSKS 127
           V  + +  K+ V G+K DP ++  ++  KT ++V L+SP P       P        ++ 
Sbjct: 103 VMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSPIP-------PPPPPPEKKAEE 162

Query: 128 DKKTDEKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGT 187
           DK   E+KK + P V T VLKV +HC+ C  +I +   + KGV     + +   VTVKG 
Sbjct: 163 DKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGV 222

Query: 188 MDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGG 247
            + + L E + +R  +  AI+                  G+ KE   E GEGE+K  EG 
Sbjct: 223 FEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEKKEE--EKGEGESKGEEGK 282

Query: 248 GGGKK----KKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGYGYGY 307
               K    KK+G GG G G   +  GG   G  +E  K+E           N  Y Y Y
Sbjct: 283 DDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIE-----------NPYYYYYY 342

Query: 308 GPGGL------VGENLHAPQIFSDENPNACSIM 325
            P  +      +  + + PQ+FSDENPNAC++M
Sbjct: 343 QPPRVAIPPMEMPPHAYPPQLFSDENPNACTVM 355

BLAST of MS013642 vs. ExPASy TrEMBL
Match: A0A5A7U7D8 (Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold60G002580 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.5e-112
Identity = 246/332 (74.10%), Postives = 270/332 (81.33%), Query Frame = 0

Query: 3   QAVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTV 62
           +AVQKKKKN+NEKNGDG GG GKKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQ+V
Sbjct: 3   EAVQKKKKNDNEKNGDGEGG-GKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQSV 62

Query: 63  KAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNN 122
           KAEI+ NKLTVMGKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N
Sbjct: 63  KAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSN 122

Query: 123 TSKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLV 182
            +K DKKTDE KKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGV EMSV+RQKD V
Sbjct: 123 NNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDSV 182

Query: 183 TVKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETK 242
            VKGTMD+KAL  SL+ERLKRPV I+PAKKEKEK+  NNK    G + +     G+G+  
Sbjct: 183 MVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEKEKENNKNEGGGGDDKKDSPTGDGDGN 242

Query: 243 APEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY--- 302
               G GG KKKK GG GGGG G  E+GGGGGGGKMEGNKMEYMGM G GYG  YGY   
Sbjct: 243 GNGNGNGGGKKKKKGGNGGGGEG--EEGGGGGGGKMEGNKMEYMGMGGIGYGYGYGYGLN 302

Query: 303 -GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
            GYGYGPGGLVGENLHAPQ+FSDENPNAC IM
Sbjct: 303 TGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of MS013642 vs. ExPASy TrEMBL
Match: A0A1S3CIH0 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103501337 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.5e-112
Identity = 246/332 (74.10%), Postives = 270/332 (81.33%), Query Frame = 0

Query: 3   QAVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTV 62
           +AVQKKKKN+NEKNGDG GG GKKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQ+V
Sbjct: 3   EAVQKKKKNDNEKNGDGEGG-GKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQSV 62

Query: 63  KAEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNN 122
           KAEI+ NKLTVMGKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N
Sbjct: 63  KAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSN 122

Query: 123 TSKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLV 182
            +K DKKTDE KKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGV EMSV+RQKD V
Sbjct: 123 NNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDSV 182

Query: 183 TVKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETK 242
            VKGTMD+KAL  SL+ERLKRPV I+PAKKEKEK+  NNK    G + +     G+G+  
Sbjct: 183 MVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEKEKENNKNEGGGGDDKKDSPTGDGDGN 242

Query: 243 APEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY--- 302
               G GG KKKK GG GGGG G  E+GGGGGGGKMEGNKMEYMGM G GYG  YGY   
Sbjct: 243 GNGNGNGGGKKKKKGGNGGGGEG--EEGGGGGGGKMEGNKMEYMGMGGIGYGYGYGYGLN 302

Query: 303 -GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
            GYGYGPGGLVGENLHAPQ+FSDENPNAC IM
Sbjct: 303 TGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of MS013642 vs. ExPASy TrEMBL
Match: A0A0A0LSA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G103270 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 1.2e-111
Identity = 249/336 (74.11%), Postives = 273/336 (81.25%), Query Frame = 0

Query: 4   AVQKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVK 63
           AVQKKKKN+NEKNGDGGGG GKKKEE P T+VLK+DMHCEGCANKI KCVKGFEGVQ+VK
Sbjct: 5   AVQKKKKNDNEKNGDGGGGEGKKKEEIPFTIVLKIDMHCEGCANKITKCVKGFEGVQSVK 64

Query: 64  AEIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNT 123
           AEI+ NKLTVMGKKID ++LREKL NKTKKKVDLISPQPKKEKDSKP     +D   +N 
Sbjct: 65  AEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSSNN 124

Query: 124 SKSDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVT 183
           +KSDKKTDE KKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGV EMSV++QKD V 
Sbjct: 125 NKSDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDKQKDSVM 184

Query: 184 VKGTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNG---DNKEAGGEGGEGE 243
           VKGTMD+KAL  SL+ERLKR V I+PAKKEKEK+ +NNKK   G   + K++    G+G 
Sbjct: 185 VKGTMDVKALIGSLSERLKRTVEIVPAKKEKEKEKDNNKKEGGGGGDEKKDSTTGDGDG- 244

Query: 244 TKAPEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYGLNYGY- 303
                G GGGKKKKKGG GGGG   GEE  GGGGGGKMEGNKMEYMGM G GYG  YGY 
Sbjct: 245 ----NGNGGGKKKKKGGNGGGG--DGEE--GGGGGGKMEGNKMEYMGMGGIGYGYGYGYG 304

Query: 304 -----GYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
                GYGYGP G+VGENLHAPQ+FSDENPNAC IM
Sbjct: 305 YGMNTGYGYGPSGIVGENLHAPQLFSDENPNACFIM 331

BLAST of MS013642 vs. ExPASy TrEMBL
Match: A0A6J1HJ75 (heavy metal-associated isoprenylated plant protein 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464956 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 4.3e-109
Identity = 249/335 (74.33%), Postives = 271/335 (80.90%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAE 65
           +KK KNENEKNGDGGG  GKKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQTVKAE
Sbjct: 3   EKKTKNENEKNGDGGG--GKKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQTVKAE 62

Query: 66  IEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNTSK 125
           IE NKLTV G+KID S+L EKL NKTKKKVDLISPQ KKEKDSKP      D   +N +K
Sbjct: 63  IEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSSNNNK 122

Query: 126 SDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVK 185
           SDKKT+E KKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGV EMS+++QK+LVTVK
Sbjct: 123 SDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKELVTVK 182

Query: 186 GTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNN--NGDNKEAGGEGGEGETKA 245
           GTMD+KALS  LTERLKR V I+P KKEK+KDNNNN   N   G NK+     GEG+  +
Sbjct: 183 GTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAGEGDGNS 242

Query: 246 PEGGGGGKKKKKGGGGGGGGNGGEEDGG-----GGGGGKMEGNKMEYMGM---TGYGYGL 305
            E  GGGKKKKKGG G GGG+GG + G      GGGGGKMEG KMEYMGM    GYGYGL
Sbjct: 243 NE-NGGGKKKKKGGNGNGGGDGGGDGGATGMEEGGGGGKMEGYKMEYMGMGGGIGYGYGL 302

Query: 306 NYGYGYGYGPGGLVGENLHAPQIFSDENPNACSIM 325
           N GYGYGYG GG+VGENLHAPQ+FSDENPNAC IM
Sbjct: 303 N-GYGYGYGAGGVVGENLHAPQLFSDENPNACWIM 333

BLAST of MS013642 vs. ExPASy TrEMBL
Match: A0A6J1HML5 (heavy metal-associated isoprenylated plant protein 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464956 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 1.1e-107
Identity = 246/330 (74.55%), Postives = 266/330 (80.61%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAE 65
           +KK KNENEKNGDGGG  GKKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQTVKAE
Sbjct: 3   EKKTKNENEKNGDGGG--GKKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQTVKAE 62

Query: 66  IEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKP-----NDDAQNNTSK 125
           IE NKLTV G+KID S+L EKL NKTKKKVDLISPQ KKEKDSKP      D   +N +K
Sbjct: 63  IEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSSNNNK 122

Query: 126 SDKKTDE-KKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVK 185
           SDKKT+E KKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGV EMS+++QK+LVTVK
Sbjct: 123 SDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKELVTVK 182

Query: 186 GTMDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNN--NGDNKEAGGEGGEGETKA 245
           GTMD+KALS  LTERLKR V I+P KKEK+KDNNNN   N   G NK+     GEG+  +
Sbjct: 183 GTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAGEGDGNS 242

Query: 246 PEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGM---TGYGYGLNYGYG 305
            E GGG KKKK   GG GG  G EE   GGGGGKMEG KMEYMGM    GYGYGLN GYG
Sbjct: 243 NENGGGKKKKK---GGNGGATGMEE---GGGGGKMEGYKMEYMGMGGGIGYGYGLN-GYG 302

Query: 306 YGYGPGGLVGENLHAPQIFSDENPNACSIM 325
           YGYG GG+VGENLHAPQ+FSDENPNAC IM
Sbjct: 303 YGYGAGGVVGENLHAPQLFSDENPNACWIM 323

BLAST of MS013642 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 241.9 bits (616), Expect = 7.2e-64
Identity = 168/326 (51.53%), Postives = 211/326 (64.72%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENP-VTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKA 65
           +KK + +N+K     GG  KKK E P +TVVLKVDMHCEGCA++I+KCV+ F+GV+TVK+
Sbjct: 3   EKKNEGDNKKK----GGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKS 62

Query: 66  EIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSK---PNDDAQNNTSKS 125
           E    KLTV G  +DP +LREKL  KTKKKVDL+SPQPKKEK+ +    ND+ +  + + 
Sbjct: 63  ESATGKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEK 122

Query: 126 DKKTDEKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGT 185
            K  +  KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV+ ++++++K+L+TVKGT
Sbjct: 123 KKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGT 182

Query: 186 MDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGG 245
           MD+K L E L+E+LKR V I+P KKEK+K+N              G E GE         
Sbjct: 183 MDVKKLVEILSEKLKRAVEIVPPKKEKDKEN--------------GNENGE--------- 242

Query: 246 GGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEG-NKMEYMGMTGYGYGLNYGYGYGYGPG 305
                KKKGGGG GGG   E+ G  GGG   EG N MEYM            YGYGY PG
Sbjct: 243 -----KKKGGGGDGGGK--EKTGNKGGG---EGVNMMEYM-------AAQPAYGYGYYPG 283

Query: 306 GLVGENL--HAPQIFSDENPNACSIM 325
           G  G  +  HAPQIFSDENPNAC +M
Sbjct: 303 GPYGYPIQAHAPQIFSDENPNACVVM 283

BLAST of MS013642 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.1e-63
Identity = 167/326 (51.23%), Postives = 211/326 (64.72%), Query Frame = 0

Query: 6   QKKKKNENEKNGDGGGGAGKKKEENP-VTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKA 65
           +KK + +N+K     GG  KKK E P +TVVLKVDMHCEGCA++I+KCV+ F+GV+TVK+
Sbjct: 3   EKKNEGDNKKK----GGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKS 62

Query: 66  EIEANKLTVMGKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSK---PNDDAQNNTSKS 125
           E    KLTV G  +DP +LREKL  KTKKKVDL+SPQPKKEK+ +    ND+ +  + + 
Sbjct: 63  ESATGKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEK 122

Query: 126 DKKTDEKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGT 185
            K  +  KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV+ ++++++K+L+TVKGT
Sbjct: 123 KKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGT 182

Query: 186 MDIKALSESLTERLKRPVAIIPAKKEKEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGG 245
           MD+K L E L+E+LKR V I+P KKEK+K+N              G E GE         
Sbjct: 183 MDVKKLVEILSEKLKRAVEIVPPKKEKDKEN--------------GNENGE--------- 242

Query: 246 GGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEG-NKMEYMGMTGYGYGLNYGYGYGYGPG 305
                KKKGGGG GGG   E+ G  GGG   EG N MEYM            YGYGY PG
Sbjct: 243 -----KKKGGGGDGGGK--EKTGNKGGG---EGVNMMEYM-------AAQPAYGYGYYPG 283

Query: 306 GLVGENL--HAPQIFSDENPNACSIM 325
           G  G  +  HAPQIFSDENPNAC ++
Sbjct: 303 GPYGYPIQAHAPQIFSDENPNACVVI 283

BLAST of MS013642 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-34
Identity = 140/355 (39.44%), Postives = 184/355 (51.83%), Query Frame = 0

Query: 20  GGGAGKKKEENPVTVVL-----KVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVM 79
           GGG        PV  V+     KVDMHCEGCA KI + VK F+GV+ V A+   NKL V+
Sbjct: 33  GGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVV 92

Query: 80  GKKIDPSQLREKLFNKTKKKVDLISPQPKKEKDSKPNDDAQNNTSKSDKKTDEKKKPKEP 139
           G KIDP +L+EKL  KTK+KV L +P PK E         +       +       P  P
Sbjct: 93  G-KIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAP 152

Query: 140 PVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGTMDIKALSESLTER 199
             +   LK+ LHC+GCI+KI ++  K KGV  ++++  KD+VTVKGT+D+K L   LT++
Sbjct: 153 KESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKK 212

Query: 200 LKRPV-AIIPAKKEKEKDNNNNKKNNNGDNK---------EAGGEGGEGETKAPEGGGGG 259
           LKR V  ++PAKK+     N   +    D K         EA  EG +G  K  E G GG
Sbjct: 213 LKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGG 272

Query: 260 KKKKKGGGGG----GGGNGGE--EDGGGGGGGKMEGNKMEYMGMT-----------GYGY 319
           +KKK+GG GG      G+GGE  +DGGG        NKM+Y G +           G+ Y
Sbjct: 273 EKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVY 332

Query: 320 GLNY---------------GYGYGYGPGGLV---GENLHAPQIFSDENPNACSIM 325
           G +Y               G GY Y     V     N++AP +FSDENPN CS+M
Sbjct: 333 GQSYSMTGQNYPVGGQSYPGSGYNYASESYVPYAQPNVNAPGMFSDENPNGCSVM 386

BLAST of MS013642 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-33
Identity = 138/392 (35.20%), Postives = 191/392 (48.72%), Query Frame = 0

Query: 22  GAGKKKEENPVTVVLKVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVMGKKIDPS 81
           G  K  +    TVV+K+DMHCEGC  KI +  K F+GV+ VK + ++NKLTV+G  +DP 
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIG-NVDPV 73

Query: 82  QLREKLFNKTKKKVDLIS--PQPKKE---------------KDSKPNDDAQNNTSKSDKK 141
           ++R+K+ +K K+ V+L+S    PKKE                + KP +       K  +K
Sbjct: 74  EVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEK 133

Query: 142 TDEKKK---------PKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDL 201
            +EKKK         P  P  +T VLK  LHC+GC  KI R+ +K KGV+ ++++  KDL
Sbjct: 134 KEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDL 193

Query: 202 VTVKGTMDIKALSESLTERLKRPVAIIPAKKE--------KEKDNNNNKKNNNGDNKEAG 261
           V VKG +D+K L+  L E+LKR V ++PAKK+                KK+     K+  
Sbjct: 194 VIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEI 253

Query: 262 GEGGEGETKAPEGGGGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGY 321
            + GE   K  +GGG  KK+   GGGGGGG      GGGG GG M+  K EY    GYGY
Sbjct: 254 KDVGE---KKVDGGGEKKKEVAVGGGGGGG------GGGGDGGAMDVKKSEY---NGYGY 313

Query: 322 G-------------------------------------------LNYGYGYGYG----PG 325
                                                       +N GYG GYG    P 
Sbjct: 314 PPQPMYYYPEGQVYGQQHYMMQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPP 373

BLAST of MS013642 vs. TAIR 10
Match: AT5G03380.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 3.2e-32
Identity = 133/378 (35.19%), Postives = 185/378 (48.94%), Query Frame = 0

Query: 36  LKVDMHCEGCANKIIKCVKGFEGVQTVKAEIEANKLTVMGKKIDPSQLREKLFNKTKKKV 95
           +K+DMHCEGC  KI +  K F+GV+ VK + ++NKLTV+G  +DP ++R+K+ +K K+ V
Sbjct: 1   MKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIG-NVDPVEVRDKVADKIKRPV 60

Query: 96  DLIS--PQPKKE---------------KDSKPNDDAQNNTSKSDKKTDEKKK-------- 155
           +L+S    PKKE                + KP +       K  +K +EKKK        
Sbjct: 61  ELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKAS 120

Query: 156 -PKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVHEMSVERQKDLVTVKGTMDIKALSE 215
            P  P  +T VLK  LHC+GC  KI R+ +K KGV+ ++++  KDLV VKG +D+K L+ 
Sbjct: 121 PPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTP 180

Query: 216 SLTERLKRPVAIIPAKKE--------KEKDNNNNKKNNNGDNKEAGGEGGEGETKAPEGG 275
            L E+LKR V ++PAKK+                KK+     K+   + GE   K  +GG
Sbjct: 181 YLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIKDVGE---KKVDGG 240

Query: 276 GGGKKKKKGGGGGGGGNGGEEDGGGGGGGKMEGNKMEYMGMTGYGYG------------- 325
           G  KK+   GGGGGGG      GGGG GG M+  K EY    GYGY              
Sbjct: 241 GEKKKEVAVGGGGGGG------GGGGDGGAMDVKKSEY---NGYGYPPQPMYYYPEGQVY 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008463119.11.1e-11174.10PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis me... [more]
XP_004147287.12.5e-11174.11heavy metal-associated isoprenylated plant protein 3 [Cucumis sativus] >KGN64800... [more]
XP_038894057.19.6e-11175.83heavy metal-associated isoprenylated plant protein 3-like [Benincasa hispida][more]
XP_022965017.19.0e-10974.33heavy metal-associated isoprenylated plant protein 3 isoform X1 [Cucurbita mosch... [more]
KAG6583560.14.4e-10872.75Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
Q9FJH51.0e-6251.53Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
Q9ZUV19.8e-3442.34Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=... [more]
Q9SJL28.3e-3339.44Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=... [more]
Q9LZF11.9e-3235.20Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=... [more]
Q9C5D36.6e-2234.23Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A5A7U7D85.5e-11274.10Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. m... [more]
A0A1S3CIH05.5e-11274.10heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=365... [more]
A0A0A0LSA41.2e-11174.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G103270 PE=4 SV=1[more]
A0A6J1HJ754.3e-10974.33heavy metal-associated isoprenylated plant protein 3 isoform X1 OS=Cucurbita mos... [more]
A0A6J1HML51.1e-10774.55heavy metal-associated isoprenylated plant protein 3 isoform X2 OS=Cucurbita mos... [more]
Match NameE-valueIdentityDescription
AT5G60800.17.2e-6451.53Heavy metal transport/detoxification superfamily protein [more]
AT5G60800.22.1e-6351.23Heavy metal transport/detoxification superfamily protein [more]
AT2G36950.15.9e-3439.44Heavy metal transport/detoxification superfamily protein [more]
AT5G03380.11.3e-3335.20Heavy metal transport/detoxification superfamily protein [more]
AT5G03380.23.2e-3235.19Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 36..87
e-value: 2.8E-10
score: 40.4
coord: 141..191
e-value: 1.7E-8
score: 34.8
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 40..87
score: 9.057206
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 35..95
e-value: 2.36021E-7
score: 45.2893
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 140..180
e-value: 0.0011766
score: 34.8889
NoneNo IPR availableGENE3D3.30.70.100coord: 136..210
e-value: 5.2E-14
score: 54.1
coord: 31..106
e-value: 5.0E-19
score: 70.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 200..269
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 200..218
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..137
NoneNo IPR availablePANTHERPTHR46413:SF2HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 3coord: 7..324
IPR044594Heavy metal-associated isoprenylated plant protein 1/3/5/6PANTHERPTHR46413HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 6coord: 7..324
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 33..89
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 136..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013642.1MS013642.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding