MS013551 (gene) Bitter gourd (TR) v1

Overview


Name	MS013551
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase domain-containing protein
Location	scaffold402: 2378287 .. 2378742 (-)
RNA-Seq Expression	MS013551
Synteny	MS013551

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGGTTGGCTGACGCGCCACCGCATCGCCGTGGGGATCGCGCGTGGGTTGGCGTATCTCCACCACGCGGGATCCAAAGCGATCGTTCACGGGCATCTGGTGACGTCCAACGTCTTGCTGGGCGACGACTTCGAGCCACGAATCGGCGGCTTTGGGTTCCGACACGTGGGCGGCGGCGGGGAGAGCTGCAGCGTGGAGAAAGACGTGTACTGTTTCGGAGTGGTGTTGATGGAGCTGGTGACGGGGATGCCGGGGACGGCGGACACGGTGGTGGGCGTGAGAAGGATGGTGAGAGAGGGAAAGGCTTTGGACGCAGTAGACCCAAGGCTGCGACTCGGCGGCGCGGAATCGGAGATGGTGGAGAGCCTCCGCGTGGCGTACCTGTGCACGGCGGAGACGGCCGTGAAAAGGCCCAACATGCAGCAGGTTCTAGGCCTACTCAAGGACATACATCCT

mRNA sequence

CTGGGTTGGCTGACGCGCCACCGCATCGCCGTGGGGATCGCGCGTGGGTTGGCGTATCTCCACCACGCGGGATCCAAAGCGATCGTTCACGGGCATCTGGTGACGTCCAACGTCTTGCTGGGCGACGACTTCGAGCCACGAATCGGCGGCTTTGGGTTCCGACACGTGGGCGGCGGCGGGGAGAGCTGCAGCGTGGAGAAAGACGTGTACTGTTTCGGAGTGGTGTTGATGGAGCTGGTGACGGGGATGCCGGGGACGGCGGACACGGTGGTGGGCGTGAGAAGGATGGTGAGAGAGGGAAAGGCTTTGGACGCAGTAGACCCAAGGCTGCGACTCGGCGGCGCGGAATCGGAGATGGTGGAGAGCCTCCGCGTGGCGTACCTGTGCACGGCGGAGACGGCCGTGAAAAGGCCCAACATGCAGCAGGTTCTAGGCCTACTCAAGGACATACATCCT

Coding sequence (CDS)

CTGGGTTGGCTGACGCGCCACCGCATCGCCGTGGGGATCGCGCGTGGGTTGGCGTATCTCCACCACGCGGGATCCAAAGCGATCGTTCACGGGCATCTGGTGACGTCCAACGTCTTGCTGGGCGACGACTTCGAGCCACGAATCGGCGGCTTTGGGTTCCGACACGTGGGCGGCGGCGGGGAGAGCTGCAGCGTGGAGAAAGACGTGTACTGTTTCGGAGTGGTGTTGATGGAGCTGGTGACGGGGATGCCGGGGACGGCGGACACGGTGGTGGGCGTGAGAAGGATGGTGAGAGAGGGAAAGGCTTTGGACGCAGTAGACCCAAGGCTGCGACTCGGCGGCGCGGAATCGGAGATGGTGGAGAGCCTCCGCGTGGCGTACCTGTGCACGGCGGAGACGGCCGTGAAAAGGCCCAACATGCAGCAGGTTCTAGGCCTACTCAAGGACATACATCCT

Protein sequence

LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGGESCSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP

Homology

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BLAST of MS013551 vs. NCBI nr
Match: KAG7012482.1 (Calmodulin-binding receptor kinase CaMRLK, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.2 bits (692), Expect = 5.5e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 497 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 556

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKALDA+DPRLRLGG ESEM
Sbjct: 557 DNCGGVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALDAIDPRLRLGGGESEM 616

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETA+KRP MQQV GLL+DIHP
Sbjct: 617 VESLRVAYLCTAETALKRPTMQQVRGLLQDIHP 647

[Close]

BLAST of MS013551 vs. NCBI nr
Match: KAG6573315.1 (Calmodulin-binding receptor kinase CaMRLK, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 271.2 bits (692), Expect = 5.5e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 526 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 585

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKALDA+DPRLRLGG ESEM
Sbjct: 586 DNCGGVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALDAIDPRLRLGGGESEM 645

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETA+KRP MQQV GLL+DIHP
Sbjct: 646 VESLRVAYLCTAETALKRPTMQQVRGLLQDIHP 676

[Close]

BLAST of MS013551 vs. NCBI nr
Match: XP_022955296.1 (calmodulin-binding receptor kinase CaMRLK-like [Cucurbita moschata])

HSP 1 Score: 271.2 bits (692), Expect = 5.5e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 526 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 585

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKALDA+DPRLRLGG ESEM
Sbjct: 586 DNCGGVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALDAIDPRLRLGGGESEM 645

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETA+KRP MQQV GLL+DIHP
Sbjct: 646 VESLRVAYLCTAETALKRPTMQQVRGLLQDIHP 676

[Close]

BLAST of MS013551 vs. NCBI nr
Match: XP_022994749.1 (calmodulin-binding receptor kinase CaMRLK-like [Cucurbita maxima])

HSP 1 Score: 270.8 bits (691), Expect = 7.2e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 528 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 587

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKAL+A+DPRLRLGG ESEM
Sbjct: 588 DNCGEVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALEAIDPRLRLGGGESEM 647

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETAVKRP MQQV GLL+DIHP
Sbjct: 648 VESLRVAYLCTAETAVKRPTMQQVRGLLQDIHP 678

[Close]

BLAST of MS013551 vs. NCBI nr
Match: XP_023541823.1 (calmodulin-binding receptor kinase CaMRLK-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 269.6 bits (688), Expect = 1.6e-68
Identity = 133/152 (87.50%), Postives = 140/152 (92.11%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHVGG  
Sbjct: 530 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHVGGDN 589

Query: 61  ESCSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEMV 120
               VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKAL+A+DPRLRLGG ESEMV
Sbjct: 590 YG-GVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALEAIDPRLRLGGGESEMV 649

Query: 121 ESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           ESLRVAYLCTAETAVKRP MQQV GLL+DIHP
Sbjct: 650 ESLRVAYLCTAETAVKRPTMQQVRGLLQDIHP 680

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BLAST of MS013551 vs. ExPASy Swiss-Prot
Match: Q9FK63 (Calmodulin-binding receptor kinase CaMRLK OS=Arabidopsis thaliana OX=3702 GN=CAMRLK PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 7.8e-42
Identity = 89/153 (58.17%), Postives = 109/153 (71.24%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGGES 62
           WL RHRIA+G+ARGLAYLHH G+    HGHLV +N+LL +  EPRI  FG  ++   G+ 
Sbjct: 512 WLIRHRIAIGVARGLAYLHHVGT---THGHLVATNILLTETLEPRISDFGINNIARTGDD 571

Query: 63  C---SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAES-- 122
               +VE DVY FGV+L EL+TG  G+ + V  VRR+V+E +  +A+D RLRL   ES  
Sbjct: 572 TNKNNVEFDVYSFGVILFELLTGKQGSDENVKSVRRLVKERRGEEALDSRLRLAAGESVN 631

Query: 123 EMVESLRVAYLCTAETAVKRPNMQQVLGLLKDI 151
           EMVESLR+ Y CTAET VKRP MQQVLGLLKDI
Sbjct: 632 EMVESLRIGYFCTAETPVKRPTMQQVLGLLKDI 661

[Close]

BLAST of MS013551 vs. ExPASy Swiss-Prot
Match: C0LGK9 (Probable LRR receptor-like serine/threonine-protein kinase At2g24230 OS=Arabidopsis thaliana OX=3702 GN=At2g24230 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.0e-30
Identity = 75/181 (41.44%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG-- 62
           W  RH+IA+G AR LA+LHH  S  I+H  +  S+V L  ++EPR+  FG   V G G  
Sbjct: 669 WRFRHKIALGTARALAFLHHGCSPPIIHRDVKASSVYLDQNWEPRLSDFGLAKVFGNGLD 728

Query: 63  --------------------ESCSVEKDVYCFGVVLMELVTGMPGTAD---------TVV 122
                               E  + + DVYCFGVVL EL+TG     D          V 
Sbjct: 729 DEIIHGSPGYLPPEFLQPEHELPTPKSDVYCFGVVLFELMTGKKPIEDDYLDEKDTNLVS 788

Query: 123 GVRRMVREGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKDIH 153
            VR +VR+ +A  A+DP+++  G+E +M E+L++ YLCTA+   KRP+MQQV+GLLKDI 
Sbjct: 789 WVRSLVRKNQASKAIDPKIQETGSEEQMEEALKIGYLCTADLPSKRPSMQQVVGLLKDIE 848

[Close]

BLAST of MS013551 vs. ExPASy Swiss-Prot
Match: Q9LVN2 (Probably inactive leucine-rich repeat receptor-like protein kinase At5g58150 OS=Arabidopsis thaliana OX=3702 GN=At5g58150 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.5e-29
Identity = 72/168 (42.86%), Postives = 98/168 (58.33%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHV----GG 62
           W  RH+IA+G AR LA+LHH     +VHG +  + +LL    EPR+  FG   +      
Sbjct: 616 WRLRHKIALGTARALAFLHHGCIPPMVHGEVKAATILLDSSQEPRLADFGLVKLLDEQFP 675

Query: 63  GGESC--------------SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALD 122
           G ES               ++E DVY FGVVL+ELV+G     D V  VR +VR+G+ L 
Sbjct: 676 GSESLDGYTPPEQERNASPTLESDVYSFGVVLLELVSGKKPEGDLVNWVRGLVRQGQGLR 735

Query: 123 AVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           A+DP ++    E E+ E++++ YLCTA+   KRP MQQV+GLLKDI P
Sbjct: 736 AIDPTMQETVPEDEIAEAVKIGYLCTADLPWKRPTMQQVVGLLKDISP 783

[Close]

BLAST of MS013551 vs. ExPASy Swiss-Prot
Match: Q9LYN8 (Leucine-rich repeat receptor protein kinase EMS1 OS=Arabidopsis thaliana OX=3702 GN=EMS1 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.8e-23
Identity = 68/181 (37.57%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 1    LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGF------- 60
            L W  R +IAVG ARGLA+LHH     I+H  +  SN+LL  DFEP++  FG        
Sbjct: 1012 LDWSKRLKIAVGAARGLAFLHHGFIPHIIHRDIKASNILLDGDFEPKVADFGLARLISAC 1071

Query: 61   -RHVG--------------GGGESCSVEKDVYCFGVVLMELVTGMPGT---------ADT 120
              HV               G     + + DVY FGV+L+ELVTG   T          + 
Sbjct: 1072 ESHVSTVIAGTFGYIPPEYGQSARATTKGDVYSFGVILLELVTGKEPTGPDFKESEGGNL 1131

Query: 121  VVGVRRMVREGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKD 151
            V    + + +GKA+D +DP L     ++  +  L++A LC AET  KRPNM  VL  LK+
Sbjct: 1132 VGWAIQKINQGKAVDVIDPLLVSVALKNSQLRLLQIAMLCLAETPAKRPNMLDVLKALKE 1191

[Close]

BLAST of MS013551 vs. ExPASy Swiss-Prot
Match: Q9FGL5 (Receptor protein-tyrosine kinase CEPR1 OS=Arabidopsis thaliana OX=3702 GN=CEPR1 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.9e-20
Identity = 65/185 (35.14%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHV--GG 60
           L W TRH+IAVG+A+GLAYLHH  S  I+H  + ++N+LL  +++P++  FG   V    
Sbjct: 757 LEWRTRHQIAVGVAQGLAYLHHDLSPPIIHRDIKSTNILLDVNYQPKVADFGIAKVLQAR 816

Query: 61  GGES---------------------CSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMV 120
           G +S                      +++ DVY FGVVLMEL+TG     D+  G  + +
Sbjct: 817 GKDSTTTVMAGTYGYLAPEYAYSSKATIKCDVYSFGVVLMELITGKK-PVDSCFGENKNI 876

Query: 121 ----------REGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLL 153
                     +EG  ++ +D RL    ++++M+ +LRVA  CT+ T   RP M +V+ LL
Sbjct: 877 VNWVSTKIDTKEG-LIETLDKRLS-ESSKADMINALRVAIRCTSRTPTIRPTMNEVVQLL 936

[Close]

BLAST of MS013551 vs. ExPASy TrEMBL
Match: A0A6J1GTE2 (calmodulin-binding receptor kinase CaMRLK-like OS=Cucurbita moschata OX=3662 GN=LOC111457300 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.7e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 526 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 585

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKALDA+DPRLRLGG ESEM
Sbjct: 586 DNCGGVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALDAIDPRLRLGGGESEM 645

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETA+KRP MQQV GLL+DIHP
Sbjct: 646 VESLRVAYLCTAETALKRPTMQQVRGLLQDIHP 676

[Close]

BLAST of MS013551 vs. ExPASy TrEMBL
Match: A0A6J1K3R3 (calmodulin-binding receptor kinase CaMRLK-like OS=Cucurbita maxima OX=3661 GN=LOC111490388 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.5e-69
Identity = 134/153 (87.58%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGWLTRHRIAVGIARGLAYLHHA SK I+HGHLVTSN+LL DDFEPRIGGFGFRHV  GG
Sbjct: 528 LGWLTRHRIAVGIARGLAYLHHARSKPIIHGHLVTSNILLADDFEPRIGGFGFRHV--GG 587

Query: 61  ESC-SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEM 120
           ++C  VEKDVYCFGVVLMEL+TGMPG+ADTVVGVRRMVREGKAL+A+DPRLRLGG ESEM
Sbjct: 588 DNCGEVEKDVYCFGVVLMELLTGMPGSADTVVGVRRMVREGKALEAIDPRLRLGGGESEM 647

Query: 121 VESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           VESLRVAYLCTAETAVKRP MQQV GLL+DIHP
Sbjct: 648 VESLRVAYLCTAETAVKRPTMQQVRGLLQDIHP 678

[Close]

BLAST of MS013551 vs. ExPASy TrEMBL
Match: A0A5A7UQY0 (Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002350 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.5e-67
Identity = 130/152 (85.53%), Postives = 138/152 (90.79%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGW TRHRIAVGIARGLAYLHHAGSK IVHGHLVTSN+LL DDFE RIGGFG RHVGG  
Sbjct: 532 LGWATRHRIAVGIARGLAYLHHAGSKPIVHGHLVTSNILLADDFEARIGGFGLRHVGGKN 591

Query: 61  ESCSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEMV 120
               VEKDVYCFGVVLMEL+TGMPG+A+TVVGVR+MVR+GKAL+ +D RLRLGGAESEMV
Sbjct: 592 GEDGVEKDVYCFGVVLMELLTGMPGSANTVVGVRKMVRDGKALEVIDQRLRLGGAESEMV 651

Query: 121 ESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           ESLRVAYLCTAETAVKRP MQQVLGLLKDIHP
Sbjct: 652 ESLRVAYLCTAETAVKRPTMQQVLGLLKDIHP 683

[Close]

BLAST of MS013551 vs. ExPASy TrEMBL
Match: A0A1S3BF56 (probable LRR receptor-like serine/threonine-protein kinase At2g24230 OS=Cucumis melo OX=3656 GN=LOC103489326 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.5e-67
Identity = 130/152 (85.53%), Postives = 138/152 (90.79%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGW TRHRIAVGIARGLAYLHHAGSK IVHGHLVTSN+LL DDFE RIGGFG RHVGG  
Sbjct: 537 LGWATRHRIAVGIARGLAYLHHAGSKPIVHGHLVTSNILLADDFEARIGGFGLRHVGGKN 596

Query: 61  ESCSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEMV 120
               VEKDVYCFGVVLMEL+TGMPG+A+TVVGVR+MVR+GKAL+ +D RLRLGGAESEMV
Sbjct: 597 GEDGVEKDVYCFGVVLMELLTGMPGSANTVVGVRKMVRDGKALEVIDQRLRLGGAESEMV 656

Query: 121 ESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           ESLRVAYLCTAETAVKRP MQQVLGLLKDIHP
Sbjct: 657 ESLRVAYLCTAETAVKRPTMQQVLGLLKDIHP 688

[Close]

BLAST of MS013551 vs. ExPASy TrEMBL
Match: A0A0A0LUM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G470410 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.6e-67
Identity = 129/152 (84.87%), Postives = 138/152 (90.79%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG 60
           LGW TRHRIAVGIARGLAYLHHAGSK IVHGHLVTSN+LL DDFE RIGGFG RHV G  
Sbjct: 532 LGWATRHRIAVGIARGLAYLHHAGSKPIVHGHLVTSNILLADDFEARIGGFGLRHVEGKK 591

Query: 61  ESCSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAESEMV 120
               VEKDVYCFGVVLMEL+TGMPG+A+TVVGVR+MVR+GKAL+A+DPRLR+GG ESEMV
Sbjct: 592 GEDGVEKDVYCFGVVLMELLTGMPGSANTVVGVRKMVRDGKALEAIDPRLRVGGGESEMV 651

Query: 121 ESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           ESLRVAYLCTAETAVKRP MQQVLGLLKDIHP
Sbjct: 652 ESLRVAYLCTAETAVKRPTMQQVLGLLKDIHP 683

[Close]

BLAST of MS013551 vs. TAIR 10
Match: AT5G45800.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 171.4 bits (433), Expect = 5.6e-43
Identity = 89/153 (58.17%), Postives = 109/153 (71.24%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGGES 62
           WL RHRIA+G+ARGLAYLHH G+    HGHLV +N+LL +  EPRI  FG  ++   G+ 
Sbjct: 512 WLIRHRIAIGVARGLAYLHHVGT---THGHLVATNILLTETLEPRISDFGINNIARTGDD 571

Query: 63  C---SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALDAVDPRLRLGGAES-- 122
               +VE DVY FGV+L EL+TG  G+ + V  VRR+V+E +  +A+D RLRL   ES  
Sbjct: 572 TNKNNVEFDVYSFGVILFELLTGKQGSDENVKSVRRLVKERRGEEALDSRLRLAAGESVN 631

Query: 123 EMVESLRVAYLCTAETAVKRPNMQQVLGLLKDI 151
           EMVESLR+ Y CTAET VKRP MQQVLGLLKDI
Sbjct: 632 EMVESLRIGYFCTAETPVKRPTMQQVLGLLKDI 661

[Close]

BLAST of MS013551 vs. TAIR 10
Match: AT2G24230.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 132.5 bits (332), Expect = 2.9e-31
Identity = 75/181 (41.44%), Postives = 102/181 (56.35%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHVGGGG-- 62
           W  RH+IA+G AR LA+LHH  S  I+H  +  S+V L  ++EPR+  FG   V G G  
Sbjct: 669 WRFRHKIALGTARALAFLHHGCSPPIIHRDVKASSVYLDQNWEPRLSDFGLAKVFGNGLD 728

Query: 63  --------------------ESCSVEKDVYCFGVVLMELVTGMPGTAD---------TVV 122
                               E  + + DVYCFGVVL EL+TG     D          V 
Sbjct: 729 DEIIHGSPGYLPPEFLQPEHELPTPKSDVYCFGVVLFELMTGKKPIEDDYLDEKDTNLVS 788

Query: 123 GVRRMVREGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKDIH 153
            VR +VR+ +A  A+DP+++  G+E +M E+L++ YLCTA+   KRP+MQQV+GLLKDI 
Sbjct: 789 WVRSLVRKNQASKAIDPKIQETGSEEQMEEALKIGYLCTADLPSKRPSMQQVVGLLKDIE 848

[Close]

BLAST of MS013551 vs. TAIR 10
Match: AT5G58150.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 129.0 bits (323), Expect = 3.2e-30
Identity = 72/168 (42.86%), Postives = 98/168 (58.33%), Query Frame = 0


Query: 3   WLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHV----GG 62
           W  RH+IA+G AR LA+LHH     +VHG +  + +LL    EPR+  FG   +      
Sbjct: 616 WRLRHKIALGTARALAFLHHGCIPPMVHGEVKAATILLDSSQEPRLADFGLVKLLDEQFP 675

Query: 63  GGESC--------------SVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMVREGKALD 122
           G ES               ++E DVY FGVVL+ELV+G     D V  VR +VR+G+ L 
Sbjct: 676 GSESLDGYTPPEQERNASPTLESDVYSFGVVLLELVSGKKPEGDLVNWVRGLVRQGQGLR 735

Query: 123 AVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKDIHP 153
           A+DP ++    E E+ E++++ YLCTA+   KRP MQQV+GLLKDI P
Sbjct: 736 AIDPTMQETVPEDEIAEAVKIGYLCTADLPWKRPTMQQVVGLLKDISP 783

[Close]

BLAST of MS013551 vs. TAIR 10
Match: AT5G07280.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 109.0 bits (271), Expect = 3.4e-24
Identity = 68/181 (37.57%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 1    LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGF------- 60
            L W  R +IAVG ARGLA+LHH     I+H  +  SN+LL  DFEP++  FG        
Sbjct: 1012 LDWSKRLKIAVGAARGLAFLHHGFIPHIIHRDIKASNILLDGDFEPKVADFGLARLISAC 1071

Query: 61   -RHVG--------------GGGESCSVEKDVYCFGVVLMELVTGMPGT---------ADT 120
              HV               G     + + DVY FGV+L+ELVTG   T          + 
Sbjct: 1072 ESHVSTVIAGTFGYIPPEYGQSARATTKGDVYSFGVILLELVTGKEPTGPDFKESEGGNL 1131

Query: 121  VVGVRRMVREGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLLKD 151
            V    + + +GKA+D +DP L     ++  +  L++A LC AET  KRPNM  VL  LK+
Sbjct: 1132 VGWAIQKINQGKAVDVIDPLLVSVALKNSQLRLLQIAMLCLAETPAKRPNMLDVLKALKE 1191

[Close]

BLAST of MS013551 vs. TAIR 10
Match: AT5G49660.1 (Leucine-rich repeat transmembrane protein kinase family protein )

HSP 1 Score: 99.0 bits (245), Expect = 3.5e-21
Identity = 65/185 (35.14%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 1   LGWLTRHRIAVGIARGLAYLHHAGSKAIVHGHLVTSNVLLGDDFEPRIGGFGFRHV--GG 60
           L W TRH+IAVG+A+GLAYLHH  S  I+H  + ++N+LL  +++P++  FG   V    
Sbjct: 757 LEWRTRHQIAVGVAQGLAYLHHDLSPPIIHRDIKSTNILLDVNYQPKVADFGIAKVLQAR 816

Query: 61  GGES---------------------CSVEKDVYCFGVVLMELVTGMPGTADTVVGVRRMV 120
           G +S                      +++ DVY FGVVLMEL+TG     D+  G  + +
Sbjct: 817 GKDSTTTVMAGTYGYLAPEYAYSSKATIKCDVYSFGVVLMELITGKK-PVDSCFGENKNI 876

Query: 121 ----------REGKALDAVDPRLRLGGAESEMVESLRVAYLCTAETAVKRPNMQQVLGLL 153
                     +EG  ++ +D RL    ++++M+ +LRVA  CT+ T   RP M +V+ LL
Sbjct: 877 VNWVSTKIDTKEG-LIETLDKRLS-ESSKADMINALRVAIRCTSRTPTIRPTMNEVVQLL 936

The following BLAST results are available for this feature:

BLAST of MS013551 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012482.1	5.5e-69	87.58	Calmodulin-binding receptor kinase CaMRLK, partial [Cucurbita argyrosperma subsp...	[more]
KAG6573315.1	5.5e-69	87.58	Calmodulin-binding receptor kinase CaMRLK, partial [Cucurbita argyrosperma subsp...	[more]
XP_022955296.1	5.5e-69	87.58	calmodulin-binding receptor kinase CaMRLK-like [Cucurbita moschata]	[more]
XP_022994749.1	7.2e-69	87.58	calmodulin-binding receptor kinase CaMRLK-like [Cucurbita maxima]	[more]
XP_023541823.1	1.6e-68	87.50	calmodulin-binding receptor kinase CaMRLK-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS013551 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FK63	7.8e-42	58.17	Calmodulin-binding receptor kinase CaMRLK OS=Arabidopsis thaliana OX=3702 GN=CAM...	[more]
C0LGK9	4.0e-30	41.44	Probable LRR receptor-like serine/threonine-protein kinase At2g24230 OS=Arabidop...	[more]
Q9LVN2	4.5e-29	42.86	Probably inactive leucine-rich repeat receptor-like protein kinase At5g58150 OS=...	[more]
Q9LYN8	4.8e-23	37.57	Leucine-rich repeat receptor protein kinase EMS1 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FGL5	4.9e-20	35.14	Receptor protein-tyrosine kinase CEPR1 OS=Arabidopsis thaliana OX=3702 GN=CEPR1 ...	[more]

BLAST of MS013551 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTE2	2.7e-69	87.58	calmodulin-binding receptor kinase CaMRLK-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1K3R3	3.5e-69	87.58	calmodulin-binding receptor kinase CaMRLK-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5A7UQY0	1.5e-67	85.53	Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. ...	[more]
A0A1S3BF56	1.5e-67	85.53	probable LRR receptor-like serine/threonine-protein kinase At2g24230 OS=Cucumis ...	[more]
A0A0A0LUM3	5.6e-67	84.87	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G470...	[more]

BLAST of MS013551 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45800.1	5.6e-43	58.17	Leucine-rich repeat protein kinase family protein	[more]
AT2G24230.1	2.9e-31	41.44	Leucine-rich repeat protein kinase family protein	[more]
AT5G58150.1	3.2e-30	42.86	Leucine-rich repeat protein kinase family protein	[more]
AT5G07280.1	3.4e-24	37.57	Leucine-rich repeat transmembrane protein kinase	[more]
AT5G49660.1	3.5e-21	35.14	Leucine-rich repeat transmembrane protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..59 e-value: 2.9E-12 score: 48.3 coord: 60..151 e-value: 1.0E-12 score: 49.9
None	No IPR available	PANTHER	PTHR48006:SF10	LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED	coord: 2..152
None	No IPR available	PANTHER	PTHR48006	LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED	coord: 2..152
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..152 score: 10.167306
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS013551.1	MS013551.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity