Homology
BLAST of MS013523 vs. NCBI nr
Match:
XP_022139989.1 (alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like [Momordica charantia])
HSP 1 Score: 1884.4 bits (4880), Expect = 0.0e+00
Identity = 933/933 (100.00%), Postives = 933/933 (100.00%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS
Sbjct: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV
Sbjct: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN
Sbjct: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV
Sbjct: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS
Sbjct: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
Query: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC
Sbjct: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
Query: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
AVGRKRSNARYLLGSSDDVVKLVKELAECSASS
Sbjct: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 933
BLAST of MS013523 vs. NCBI nr
Match:
XP_038894955.1 (alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1740.7 bits (4507), Expect = 0.0e+00
Identity = 866/934 (92.72%), Postives = 893/934 (95.61%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNRSSSLNEEARD YSLGEVNS DLFT G S
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNRSSSLNEEARDVYSLGEVNSYDLFTYAGDS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
GSF EED+L+ +SV KD D QRPEG+ KQRLLVVANRLPVSAVR+GKETWHLEISV
Sbjct: 61 CGSFNEEDVLDRISVVKDIFDGFQRPEGKTPKQRLLVVANRLPVSAVRKGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLK+HNN MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKQHNNTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDH+KELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHIKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIGYALNM ADERERRHHHNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALNMPADERERRHHHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLP REAVN Y SHNRL+ILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPIREAVNCYSHSHNRLIILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRRY QIRDMDLKVHPDVREPLKKLCDD NTTVVVLSGS+RSVLDDNFG+YN
Sbjct: 601 LTEAVDALGRRYDQIRDMDLKVHPDVREPLKKLCDDQNTTVVVLSGSERSVLDDNFGNYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL W+
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVWS 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV
Sbjct: 721 YKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPF-GVPVPKLPN 840
RDKG N+PIDYVLCVGHFLTKDEDVYTFFEPDLPS+VPP AR TV TNPF G P PK PN
Sbjct: 781 RDKGINKPIDYVLCVGHFLTKDEDVYTFFEPDLPSKVPPAAR-TVITNPFGGAPAPKNPN 840
Query: 841 SGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFS 900
+GV P S + RLKKQRSLS+LERRANSHGSAVFWRP++QDRLSLHEGSSVLDLKGDNYFS
Sbjct: 841 NGVSP-SRSARLKKQRSLSILERRANSHGSAVFWRPVVQDRLSLHEGSSVLDLKGDNYFS 900
Query: 901 CAVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
CAV RKRSNARYLLGSSDDVV LVKEL+ECS S+
Sbjct: 901 CAVRRKRSNARYLLGSSDDVVTLVKELSECSESN 932
BLAST of MS013523 vs. NCBI nr
Match:
XP_031736267.1 (alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 [Cucumis sativus] >KGN65943.2 hypothetical protein Csa_019874 [Cucumis sativus])
HSP 1 Score: 1738.8 bits (4502), Expect = 0.0e+00
Identity = 859/934 (91.97%), Postives = 892/934 (95.50%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNH+ SPPTPRTRLERLLRERELRRSN+ SSLNEEARD YSLGEVNS DLFTN G S
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNKPSSLNEEARDAYSLGEVNSYDLFTNAGDS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
GSF EED+LEG+SV KD D QRPEGR KQRLLVVANRLPVSAVR+GKETWHLEISV
Sbjct: 61 CGSFNEEDVLEGISVVKDIFDGFQRPEGRTYKQRLLVVANRLPVSAVRKGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLKEHNN MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKEHNNTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDS+RFIRALKLPQVQDH+KELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSERFIRALKLPQVQDHIKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIGYALNM ADERERRHHHNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALNMPADERERRHHHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLP REAVN Y +SHNRL+ILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPIREAVNCYSRSHNRLIILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEA+DALGRRYGQIRDMDL++HPDV+EPLKKLCDD NTTVVVLSGSDRSVLDDNFG+YN
Sbjct: 601 LTEALDALGRRYGQIRDMDLRLHPDVKEPLKKLCDDQNTTVVVLSGSDRSVLDDNFGNYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL WN
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVWN 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV
Sbjct: 721 YKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPF-GVPVPKLPN 840
RDKG N+PIDYVLCVGHFLTKDEDVYTFFEPDLP EVP A TN F GVP PK+ N
Sbjct: 781 RDKGINKPIDYVLCVGHFLTKDEDVYTFFEPDLPIEVPAAAARNTVTNSFGGVPAPKISN 840
Query: 841 SGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFS 900
+GV P S + RLKKQRSL++LE+RAN+HGSAVFWRP++QDRLSLHEGSSVLDLKGD+YFS
Sbjct: 841 NGVSP-SRSARLKKQRSLTILEKRANTHGSAVFWRPVVQDRLSLHEGSSVLDLKGDDYFS 900
Query: 901 CAVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
CAVGRKRSNARYLLGSSDDVV LVKEL+ECS S+
Sbjct: 901 CAVGRKRSNARYLLGSSDDVVTLVKELSECSVSN 933
BLAST of MS013523 vs. NCBI nr
Match:
XP_008443729.1 (PREDICTED: alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like [Cucumis melo])
HSP 1 Score: 1728.0 bits (4474), Expect = 0.0e+00
Identity = 858/932 (92.06%), Postives = 890/932 (95.49%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLN-EEARDGYSLGEVNSSDLFTNFGG 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNRSSSLN +E+RD SLGEVNS DLFTN G
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNRSSSLNDQESRDACSLGEVNSYDLFTNAGD 60
Query: 61 SYGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEIS 120
GSF EED+LEG+SV KD D QRPEGR KQRLLVVANRLPVSAVR+GKETWHLEIS
Sbjct: 61 ICGSFNEEDVLEGISVVKDSFDGFQRPEGRTYKQRLLVVANRLPVSAVRKGKETWHLEIS 120
Query: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG
Sbjct: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
Query: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY
Sbjct: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
Query: 241 HLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYAR 300
HLMFLPKCLKEHN+ MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYAR
Sbjct: 241 HLMFLPKCLKEHNSTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYAR 300
Query: 301 HFVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERF 360
HFVSACTRILGLEGTPEGVEDQGKLT IAAFPIGIDS+RFIRALKLPQVQDH+KELQERF
Sbjct: 301 HFVSACTRILGLEGTPEGVEDQGKLTHIAAFPIGIDSERFIRALKLPQVQDHIKELQERF 360
Query: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTS 420
AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTS
Sbjct: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTS 420
Query: 421 QVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV 480
QVHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV
Sbjct: 421 QVHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV 480
Query: 481 ACQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNF 540
ACQASKKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIGYAL+M ADERERRHHHNF
Sbjct: 481 ACQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALDMPADERERRHHHNF 540
Query: 541 MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNA 600
MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLP REAVN Y +SHNRLVILGFNA
Sbjct: 541 MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPIREAVNCYSRSHNRLVILGFNA 600
Query: 601 TLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDY 660
TLTEAVDALGRRYGQIRDMDL+++P V+EPLKKLCDD NTTVVVLSGSDRSVLDDNFGDY
Sbjct: 601 TLTEAVDALGRRYGQIRDMDLRLNPAVKEPLKKLCDDKNTTVVVLSGSDRSVLDDNFGDY 660
Query: 661 NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQW 720
NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL W
Sbjct: 661 NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVW 720
Query: 721 NYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI 780
NYK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI
Sbjct: 721 NYKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI 780
Query: 781 VRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPF-GVPVPKLP 840
VRDKG N+PIDYVLCVGHFLTKDEDVYTFFEPDLP+EVP T TNPF GVPVPK+P
Sbjct: 781 VRDKGINKPIDYVLCVGHFLTKDEDVYTFFEPDLPTEVPAAVARTTVTNPFGGVPVPKIP 840
Query: 841 NSGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYF 900
N+ V P S + RLKKQRSLS+LER+ANSHGSAVFWRP++QDRLSLHEGSSVLDLKGDNYF
Sbjct: 841 NTVVSP-SRSARLKKQRSLSILERKANSHGSAVFWRPVVQDRLSLHEGSSVLDLKGDNYF 900
Query: 901 SCAVGRKRSNARYLLGSSDDVVKLVKELAECS 931
SCAVGRKRSNARYL+GSSDDVV LVKEL+ECS
Sbjct: 901 SCAVGRKRSNARYLIGSSDDVVTLVKELSECS 931
BLAST of MS013523 vs. NCBI nr
Match:
XP_038894954.1 (alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1725.3 bits (4467), Expect = 0.0e+00
Identity = 866/963 (89.93%), Postives = 893/963 (92.73%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNRSSSLNEEARD YSLGEVNS DLFT G S
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNRSSSLNEEARDVYSLGEVNSYDLFTYAGDS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
GSF EED+L+ +SV KD D QRPEG+ KQRLLVVANRLPVSAVR+GKETWHLEISV
Sbjct: 61 CGSFNEEDVLDRISVVKDIFDGFQRPEGKTPKQRLLVVANRLPVSAVRKGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLK+HNN MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKQHNNTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDH+KELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHIKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIGYALNM ADERERRHHHNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALNMPADERERRHHHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLP REAVN Y SHNRL+ILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPIREAVNCYSHSHNRLIILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRRY QIRDMDLKVHPDVREPLKKLCDD NTTVVVLSGS+RSVLDDNFG+YN
Sbjct: 601 LTEAVDALGRRYDQIRDMDLKVHPDVREPLKKLCDDQNTTVVVLSGSERSVLDDNFGNYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL W+
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVWS 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV
Sbjct: 721 YKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTK-----------------------------DEDVYTFFEP 840
RDKG N+PIDYVLCVGHFLTK DEDVYTFFEP
Sbjct: 781 RDKGINKPIDYVLCVGHFLTKVYTNIFIKDPVMFFLFPCTVLINSPVMLQDEDVYTFFEP 840
Query: 841 DLPSEVPPTARATVTTNPF-GVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSA 900
DLPS+VPP AR TV TNPF G P PK PN+GV P S + RLKKQRSLS+LERRANSHGSA
Sbjct: 841 DLPSKVPPAAR-TVITNPFGGAPAPKNPNNGVSP-SRSARLKKQRSLSILERRANSHGSA 900
Query: 901 VFWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECS 934
VFWRP++QDRLSLHEGSSVLDLKGDNYFSCAV RKRSNARYLLGSSDDVV LVKEL+ECS
Sbjct: 901 VFWRPVVQDRLSLHEGSSVLDLKGDNYFSCAVRRKRSNARYLLGSSDDVVTLVKELSECS 960
BLAST of MS013523 vs. ExPASy Swiss-Prot
Match:
Q9SYM4 (Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Arabidopsis thaliana OX=3702 GN=TPS1 PE=1 SV=1)
HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 702/951 (73.82%), Postives = 793/951 (83.39%), Query Frame = 0
Query: 1 MLSNNHSCSPP-TPRTRLERLLREREL---RRSNRSSSLNEEARDGYSLGEVNSSDLFTN 60
M N ++CS P +R ERLLR+REL R+SNR+ + N+ A G +S+
Sbjct: 1 MPGNKYNCSSSHIPLSRTERLLRDRELREKRKSNRARNPNDVA------GSSENSENDLR 60
Query: 61 FGGSYGSFYEEDILEGVSVAKDFSDALQRPEGR-YSKQRLLVVANRLPVSAVRRGKETWH 120
G Y E LEG + A DA +R E R Y++QRLLVVANRLPVSAVRRG+++W
Sbjct: 61 LEGDSSRQYVEQYLEGAAAAMAHDDACERQEVRPYNRQRLLVVANRLPVSAVRRGEDSWS 120
Query: 121 LEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQ 180
LEIS GGLVSALLG+KEFEARWIGWAGVNVPDE GQ+AL KALAEK+CIPVFLDEEIVHQ
Sbjct: 121 LEISAGGLVSALLGVKEFEARWIGWAGVNVPDEVGQKALSKALAEKRCIPVFLDEEIVHQ 180
Query: 181 YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVW 240
YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQF AYKKANQMFADVVN++YEEGDVVW
Sbjct: 181 YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVW 240
Query: 241 CHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTY 300
CHDYHLMFLPKCLKE+N+KMKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTY
Sbjct: 241 CHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTY 300
Query: 301 DYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKEL 360
DYARHFVSACTRILGLEGTPEGVEDQG+LTR+AAFPIGIDSDRFIRAL++P+V H+KEL
Sbjct: 301 DYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALEVPEVIQHMKEL 360
Query: 361 QERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQ 420
+ERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN WRD+VVLLQIAVPTRTDV +YQ
Sbjct: 361 KERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENANWRDKVVLLQIAVPTRTDVPEYQ 420
Query: 421 KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS 480
KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS
Sbjct: 421 KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS 480
Query: 481 YEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRH 540
YEFVACQ +KKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIG ALNM A+ERE+RH
Sbjct: 481 YEFVACQEAKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMTAEEREKRH 540
Query: 541 HHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVIL 600
HNF HV THT+QEWA TFVSELNDT++EAQ+R ++PP LP +A+ RY +S+NRL+IL
Sbjct: 541 RHNFHHVKTHTAQEWAETFVSELNDTVIEAQLRISKVPPELPQHDAIQRYSKSNNRLLIL 600
Query: 601 GFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDN 660
GFNATLTE VD GRR QI++MDL +HP+++ PLK LC DP+TT+VVLSGS RSVLD N
Sbjct: 601 GFNATLTEPVDNQGRRGDQIKEMDLNLHPELKGPLKALCSDPSTTIVVLSGSSRSVLDKN 660
Query: 661 FGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRET 720
FG+Y+MWLAAENGMFLR T GEWMTTMPE+L+M+WV+SVKHVF+YFTERTPRSHFE R+T
Sbjct: 661 FGEYDMWLAAENGMFLRLTNGEWMTTMPEHLNMEWVDSVKHVFKYFTERTPRSHFETRDT 720
Query: 721 SLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI 780
SL WNYK+AD+EFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI
Sbjct: 721 SLIWNYKYADIEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI 780
Query: 781 LGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVP 840
LGEIV K PIDYVLC+GHFL KDEDVYTFFEP+LPS++P AR+ +++
Sbjct: 781 LGEIVHSKSMTTPIDYVLCIGHFLGKDEDVYTFFEPELPSDMPAIARSRPSSDS---GAK 840
Query: 841 KLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGS-------- 900
PP +T K S S +N++ + R + +R S S
Sbjct: 841 SSSGDRRPPSKSTHNNNKSGSKSSSSSNSNNNNKS-SQRSLQSERKSGSNHSLGNSRRPS 900
Query: 901 ------SVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECSAS 933
+VLDLKG+NYFSCAVGR R+NARYLLGS DDVV +++LA+ ++S
Sbjct: 901 PEKISWNVLDLKGENYFSCAVGRTRTNARYLLGSPDDVVCFLEKLADTTSS 941
BLAST of MS013523 vs. ExPASy Swiss-Prot
Match:
Q9FZ57 (Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 OS=Arabidopsis thaliana OX=3702 GN=TPS2 PE=3 SV=1)
HSP 1 Score: 1019.2 bits (2634), Expect = 2.9e-296
Identity = 498/842 (59.14%), Postives = 636/842 (75.53%), Query Frame = 0
Query: 87 EGRYSKQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGL-KEFEARWIGWAGVNV 146
+ R + RLLVVANRLPVSA R G+ +W LE+S GGLVS LLG+ +F+ +W+GW GV+V
Sbjct: 6 DARGERPRLLVVANRLPVSAKRTGENSWSLEMSPGGLVSGLLGITSQFDTKWVGWPGVDV 65
Query: 147 PDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRS 206
DE + AL ++LAE KCIPVFL+ + QYYNGYCN ILWP+ H++GLPQED+ T ++
Sbjct: 66 HDEIEKNALTESLAEMKCIPVFLN-GVFDQYYNGYCNGILWPILHHMGLPQEDQHDTNQT 125
Query: 207 FQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPF 266
F++Q+DAYKKAN+MF DV+ YEEGD+VWCHDYHLMFLP+ LKE+NNK+KVGWFLH+PF
Sbjct: 126 FETQYDAYKKANRMFLDVIIDNYEEGDIVWCHDYHLMFLPQYLKEYNNKIKVGWFLHSPF 185
Query: 267 PSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLT 326
PSSE+++TLPSR+ELLR++LAADL+GFHTYD+ARHF+S CTRILG+EGT EGV QG++T
Sbjct: 186 PSSEVYKTLPSRSELLRAILAADLLGFHTYDFARHFLSTCTRILGVEGTHEGVVYQGRVT 245
Query: 327 RIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFE 386
R+A FPIGID DRFIR KLP+V + ELQE+FAG+KV+LGVDRLDMIKGIPQK LAFE
Sbjct: 246 RVAVFPIGIDPDRFIRTCKLPEVTQQMNELQEKFAGKKVILGVDRLDMIKGIPQKYLAFE 305
Query: 387 KFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDR 446
KFLEENP WRD+VVL+QIAVPTR DV +Y+KL SQVH +VGRINGRFG+++++PIHHLD
Sbjct: 306 KFLEENPYWRDKVVLVQIAVPTRNDVPEYRKLKSQVHGLVGRINGRFGSVSSLPIHHLDC 365
Query: 447 SLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGA 506
S+DF+ LCA+YA+ DV LVTSLRDGMNLVSYEFVACQ +KKGVL+LSEFAGA QSLG GA
Sbjct: 366 SVDFNYLCAIYAIADVMLVTSLRDGMNLVSYEFVACQEAKKGVLVLSEFAGAGQSLGVGA 425
Query: 507 VLVNPWNITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEA 566
++VNPW++TEV+++I ALNM A+ERE RH NF +V TH++++W F+SELN I E+
Sbjct: 426 LIVNPWDVTEVSSAIKEALNMPAEERETRHRSNFQYVCTHSAEKWGLDFMSELNGIIPES 485
Query: 567 QIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPD 626
+++ R+IP LP ++ + +Y QS+NRL+ILGF TL E +++ ++MDLK++P+
Sbjct: 486 EMQMRKIPLQLPEQDVIQQYSQSNNRLIILGFFGTLAEPMNS------GTKEMDLKLNPE 545
Query: 627 VREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPEN 686
++ LK LC+DP TTVVVLS S +++L+ NFG+ N+WLAAENGMF + T GEW+T MP+N
Sbjct: 546 LKGTLKALCNDPKTTVVVLSRSGKNILNKNFGESNIWLAAENGMFEKQTTGEWVTNMPQN 605
Query: 687 LHMDWVESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPI 746
+++DWV+ VK+VF+YFT+RTPRS+FE ETSL WNY++ADVEFGR QARDLLQ+LW GPI
Sbjct: 606 VNLDWVDGVKNVFKYFTDRTPRSYFEASETSLVWNYEYADVEFGRAQARDLLQYLWAGPI 665
Query: 747 SNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDV 806
SNASVDVV+G+ SVEV A+G TKGAAI RILGEIV K PID+V C G+FL KDED+
Sbjct: 666 SNASVDVVRGNHSVEVHAIGETKGAAIGRILGEIVHRKSMTTPIDFVFCSGYFLEKDEDI 725
Query: 807 YTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRAN 866
YTFFE + S K PN
Sbjct: 726 YTFFESKILSS-------------------KSPNG------------------------- 772
Query: 867 SHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKE 926
LDLK +NYFS A+G+ R+ ARY++ S+ VV L+ +
Sbjct: 786 ------------------------LDLKKENYFSAAIGQARTKARYVIDSAHGVVDLLHK 772
Query: 927 LA 928
LA
Sbjct: 846 LA 772
BLAST of MS013523 vs. ExPASy Swiss-Prot
Match:
Q9T079 (Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4 OS=Arabidopsis thaliana OX=3702 GN=TPS4 PE=3 SV=1)
HSP 1 Score: 989.2 bits (2556), Expect = 3.2e-287
Identity = 498/846 (58.87%), Postives = 627/846 (74.11%), Query Frame = 0
Query: 91 SKQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAG 150
++ RLLVV+ LPV+A R G+E+W +S GGLVSALLGLKEFE +WIGW GV+V D G
Sbjct: 2 ARPRLLVVSMSLPVTAKRTGEESWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAIG 61
Query: 151 QRALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQF 210
++ L LAEK CIPVFL EE+ QYYNGYCNNILWP+FHYLG P E R T ++QSQ+
Sbjct: 62 KKTLSITLAEKGCIPVFL-EEVCDQYYNGYCNNILWPIFHYLGTPPEYRNDATITYQSQY 121
Query: 211 DAYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEI 270
+AYKKANQ+F DVV ++YEEGDVVWCHDYH+M LP+ LKE+N+KMKVGWFLHTPFPSSE+
Sbjct: 122 EAYKKANQIFFDVVKEHYEEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEM 181
Query: 271 HRTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAF 330
++TLPSR++LLRSVL ADLVGFHTYD+ARHF++AC ILG+E T EG+ DQGK+TR+A F
Sbjct: 182 YKTLPSRSDLLRSVLTADLVGFHTYDFARHFLNACMCILGVEATSEGIVDQGKVTRVAVF 241
Query: 331 PIGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE 390
PIGI+ +RFI +L +V ++K+ + F GRK++LGVDRLD IKGIPQK AFEKFLEE
Sbjct: 242 PIGIEPERFINTSELSEVVQYMKKFKNDFGGRKLILGVDRLDTIKGIPQKYQAFEKFLEE 301
Query: 391 NPKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFH 450
N +WR +V+LLQIAVPTR + +YQK+ Q H VGRINGRFG++++VPI HLD S+DF+
Sbjct: 302 NAEWRGKVMLLQIAVPTRNGIGEYQKIKDQCHYHVGRINGRFGSISSVPIIHLDCSIDFN 361
Query: 451 ALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNP 510
LCALYA+TDV LVTSLRDGMNLVS EF+ACQ ++KGVLILSEFAGA QSLGAGA+LVNP
Sbjct: 362 QLCALYAITDVLLVTSLRDGMNLVSSEFIACQKAEKGVLILSEFAGAGQSLGAGAILVNP 421
Query: 511 WNITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFV---------SELNDT 570
WNI EV+++IG ALNM +E+ER+H NF +V TH++Q+WA F+ S+L +
Sbjct: 422 WNIKEVSSAIGEALNMSHEEKERKHKINFQYVKTHSTQQWADDFMKLTLTNILCSKLIEI 481
Query: 571 IVEAQIRTRQIPPL-LPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDL 630
A++ L LP + + +Y +S+NRL+ILGF TLT+ + RR M+L
Sbjct: 482 TTSAELGAGLAATLELPEHDVIQQYSKSNNRLLILGFYGTLTQPMKNQERRGD---GMNL 541
Query: 631 KVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMT 690
++HP ++E LK+LC DP TTVVVLS S++ +LD NFG+YNMWLAAENGMFLR T GEW+T
Sbjct: 542 ELHPQLKERLKELCSDPKTTVVVLSRSEKCILDKNFGEYNMWLAAENGMFLRHTSGEWVT 601
Query: 691 TMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHL 750
+PE+++++W++ VKHVF+YFTERTP S+ E E SL WNY+ AD EFGR QARD+LQHL
Sbjct: 602 RIPEHMNLEWIDGVKHVFKYFTERTPGSYLETSEASLVWNYENADAEFGRAQARDMLQHL 661
Query: 751 WTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLT 810
W GPISNASVDVV+G +SVEV AVGVTKG+A++RILGEIV +K PIDYVLC+G FL
Sbjct: 662 WAGPISNASVDVVRGGQSVEVHAVGVTKGSAMERILGEIVHNKSMATPIDYVLCIGCFLG 721
Query: 811 KDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVL 870
KDEDVYTFFEP+L KK +SLS
Sbjct: 722 KDEDVYTFFEPEL-------------------------------------TKKAKSLS-- 781
Query: 871 ERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVV 927
S GS ++S S+++DLKG+NYFS A+G+ + ARY L SSDDVV
Sbjct: 782 -----SSGSD------SPKKVS----STIVDLKGENYFSVAIGQTHTKARYFLDSSDDVV 789
BLAST of MS013523 vs. ExPASy Swiss-Prot
Match:
Q9SHG0 (Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 3 OS=Arabidopsis thaliana OX=3702 GN=TPS3 PE=3 SV=3)
HSP 1 Score: 951.0 bits (2457), Expect = 9.7e-276
Identity = 473/842 (56.18%), Postives = 620/842 (73.63%), Query Frame = 0
Query: 92 KQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAGQ 151
+Q LLVVANRLP SA R G+ +W LE+S GG + L+ +
Sbjct: 10 RQTLLVVANRLPASAKRTGEHSWSLEMSPGGKFNLLV---------------------EK 69
Query: 152 RALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFD 211
A+ K+LAE KCIPVFL+ E+ QYYNGY N ILWP+ H++GLPQE T ++F++Q+D
Sbjct: 70 DAVSKSLAEMKCIPVFLN-EVFDQYYNGYSNGILWPILHHMGLPQEYDHDTIKTFETQYD 129
Query: 212 AYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIH 271
AYKKAN+MF DV+ + Y++GD+VWC DYHLMFLP+ LKE+NNK+KVGWFLH+PFPSSEI+
Sbjct: 130 AYKKANRMFLDVIKENYKDGDIVWCQDYHLMFLPQYLKEYNNKIKVGWFLHSPFPSSEIY 189
Query: 272 RTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAFP 331
+TLPSR+ELLRSVLAADL+ FHTYD+ARHFV+ CTRILG+EGT EGV QG++TR+ P
Sbjct: 190 KTLPSRSELLRSVLAADLISFHTYDFARHFVNTCTRILGVEGTHEGVVYQGRVTRVVVLP 249
Query: 332 IGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN 391
+GI +RFI+ KLP+V + EL++RF+G+KV+LGVDRLDMIKGIPQK L FEKFL+EN
Sbjct: 250 MGIYPNRFIKTCKLPEVIQQMNELKDRFSGKKVILGVDRLDMIKGIPQKYLGFEKFLDEN 309
Query: 392 PKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHA 451
P WRD++VL+QIAVPTR +V +YQKL +QVH +VGRINGRFG+++++PIHH+D S+D +
Sbjct: 310 PNWRDKIVLVQIAVPTRNEVPEYQKLKNQVHRLVGRINGRFGSVSSLPIHHMDCSVDSNY 369
Query: 452 LCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPW 511
LCALYA++DV LVTSLRDG+NLVS+EFVACQ +K+GVLILSEFAGA QSLGAGA+LVNPW
Sbjct: 370 LCALYAISDVMLVTSLRDGLNLVSHEFVACQEAKRGVLILSEFAGAGQSLGAGALLVNPW 429
Query: 512 NITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQ 571
N+TEV+++I ALNM +ERE RH NF +V TH++++W F+SELND E++++ R+
Sbjct: 430 NVTEVSSAIKKALNMPYEERETRHRVNFKYVKTHSAEKWGFDFLSELNDAFDESELQIRK 489
Query: 572 IPPLLPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLK 631
IP LP ++ + RY S+NRL+ILGF T+TE ++L ++MDLK++P+++E LK
Sbjct: 490 IPHELPQQDVIQRYSLSNNRLIILGFYGTITEPRNSLS------KEMDLKLNPELKETLK 549
Query: 632 KLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWV 691
LC+DP TTVVVLS S +++LD NFG+Y +WLAAENGMFL+ T EW+T MP+N+++DWV
Sbjct: 550 ALCNDPKTTVVVLSRSGKNILDKNFGEYKIWLAAENGMFLKHTTEEWVTNMPQNMNLDWV 609
Query: 692 ESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVD 751
+ +K+VF+YFT+RTPRS FE +TSL WNY++ADVEFGR QARDLLQ+LW GPISNAS +
Sbjct: 610 DGLKNVFKYFTDRTPRSFFEASKTSLVWNYEYADVEFGRAQARDLLQYLWAGPISNASAE 669
Query: 752 VVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEP 811
VV+G SVEV A+GVTK I ILGEIV K PIDYV C G+FL KDED+YTFFE
Sbjct: 670 VVRGKYSVEVHAIGVTKEPEIGHILGEIVHKKAMTTPIDYVFCSGYFLEKDEDIYTFFES 729
Query: 812 DLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSAV 871
++ S PKL S TR K S LE++ +
Sbjct: 730 EILS-------------------PKL--------SHETRSKSSSSNHSLEKKVS------ 775
Query: 872 FWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECSA 931
+VLDLK +NYFS A+G+ R+ ARY++ SS +VV L+ +LA +
Sbjct: 790 ---------------LNVLDLKQENYFSTAIGQARTKARYVVDSSHNVVNLLHKLAVANT 775
Query: 932 SS 934
++
Sbjct: 850 TT 775
BLAST of MS013523 vs. ExPASy Swiss-Prot
Match:
Q54K57 (Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1)
HSP 1 Score: 578.2 bits (1489), Expect = 1.7e-163
Identity = 302/720 (41.94%), Postives = 447/720 (62.08%), Query Frame = 0
Query: 94 RLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRA 153
RL+VV+NRLPVS + W ++S GGLV+AL GLK W+GW G + ++ +
Sbjct: 17 RLIVVSNRLPVSIKKESNGKWSCKMSSGGLVAALSGLKS-NFIWVGWIGAEIEEDDRKEI 76
Query: 154 LEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYL--GLPQEDRLATTRSFQSQFD 213
E + CIPVFL E++ +++YNG+ N +LWPLFHYL L +DR+ ++
Sbjct: 77 KELLWKDYSCIPVFLSEKVANEHYNGFSNGVLWPLFHYLPGDLDYDDRI---------WN 136
Query: 214 AYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIH 273
+Y +AN+ F+ VV + + D+VW HDYH+M LP+ LK+ ++G+FLH PFPSSEI
Sbjct: 137 SYVEANEQFSSVVAEILKPNDLVWVHDYHMMLLPEILKQKKPDARIGFFLHIPFPSSEIF 196
Query: 274 RTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAFP 333
R LP R E+L +L L+GFHTYDYARHF+ +CTRI+GLE P GV + + ++ FP
Sbjct: 197 RVLPCRKEILLGILNCCLIGFHTYDYARHFLKSCTRIVGLETAPNGVYFKDRFVQVGVFP 256
Query: 334 IGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN 393
+GID D+F +LK QVQ+ +KEL+E F G KV++G+DRLD IKGIPQK+ A E+ ++
Sbjct: 257 VGIDPDKFFESLKTTQVQNRIKELKESFEGTKVLIGIDRLDYIKGIPQKLQAIERLFQKY 316
Query: 394 PKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHA 453
P+W+ ++VL+Q+AVP+R DV +YQKL +V E+VGRING +G++ PIH+L +S+D
Sbjct: 317 PEWKGKLVLIQVAVPSRQDVEEYQKLKKEVEELVGRINGLYGSIGYSPIHYLFQSVDPSE 376
Query: 454 LCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPW 513
L ALY ++D AL+TS+RDGMNLV+ E++ CQ GVLILSEF GAAQSL +GAV++NPW
Sbjct: 377 LTALYNISDAALITSIRDGMNLVAQEYIVCQTENNGVLILSEFTGAAQSL-SGAVMINPW 436
Query: 514 NITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQ 573
N EVA SI +L M +ERE +H +VT HT+ W FV ELN +A T +
Sbjct: 437 NTEEVADSIHNSLLMPPEEREEKHQMLLKYVTKHTASHWGLGFVKELN----KASSNTDK 496
Query: 574 IPPL--LPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPDVREP 633
+ + L + V+ Y QS RL+I ++ TL Y + + + ++
Sbjct: 497 MVTIQKLDIEKVVDIYKQSKRRLLIFAYDGTLIP--------YNNVPQLS-RPSQELLNS 556
Query: 634 LKKLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLR-PTKGEWMTTMPENLHM 693
L +DP T V +LSG D+ L + F + L+AE G F + P EW +P ++ +
Sbjct: 557 FDILSNDPKTDVYILSGRDKKTLSEWFLGIQIGLSAEYGCFFKLPESTEWEQQVP-SMDL 616
Query: 694 DWVESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPISNA 753
W E+++ +F+YFT RTP S FE +E W+Y+ AD FG +QAR+L HL N
Sbjct: 617 SWKETIRPLFKYFTLRTPGSFFEEKEMLFTWHYRNADPIFGSIQARELHLHL-----DNL 676
Query: 754 SVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTF 809
+DV+ G +++ VR+ + +++ +++ + + KG +D +L +G ++ TF
Sbjct: 677 PLDVIVGDKTLGVRSYNINPLSSMKKVITDTI-PKG----LDLILLIGDTHIHPSELPTF 701
BLAST of MS013523 vs. ExPASy TrEMBL
Match:
A0A6J1CEH2 (Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Momordica charantia OX=3673 GN=LOC111010763 PE=3 SV=1)
HSP 1 Score: 1884.4 bits (4880), Expect = 0.0e+00
Identity = 933/933 (100.00%), Postives = 933/933 (100.00%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS
Sbjct: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV
Sbjct: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN
Sbjct: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV
Sbjct: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS
Sbjct: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
Query: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC
Sbjct: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
Query: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
AVGRKRSNARYLLGSSDDVVKLVKELAECSASS
Sbjct: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 933
BLAST of MS013523 vs. ExPASy TrEMBL
Match:
A0A1S3B8S6 (Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucumis melo OX=3656 GN=LOC103487242 PE=3 SV=1)
HSP 1 Score: 1728.0 bits (4474), Expect = 0.0e+00
Identity = 858/932 (92.06%), Postives = 890/932 (95.49%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLN-EEARDGYSLGEVNSSDLFTNFGG 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNRSSSLN +E+RD SLGEVNS DLFTN G
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNRSSSLNDQESRDACSLGEVNSYDLFTNAGD 60
Query: 61 SYGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEIS 120
GSF EED+LEG+SV KD D QRPEGR KQRLLVVANRLPVSAVR+GKETWHLEIS
Sbjct: 61 ICGSFNEEDVLEGISVVKDSFDGFQRPEGRTYKQRLLVVANRLPVSAVRKGKETWHLEIS 120
Query: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG
Sbjct: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
Query: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY
Sbjct: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
Query: 241 HLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYAR 300
HLMFLPKCLKEHN+ MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYAR
Sbjct: 241 HLMFLPKCLKEHNSTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYAR 300
Query: 301 HFVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERF 360
HFVSACTRILGLEGTPEGVEDQGKLT IAAFPIGIDS+RFIRALKLPQVQDH+KELQERF
Sbjct: 301 HFVSACTRILGLEGTPEGVEDQGKLTHIAAFPIGIDSERFIRALKLPQVQDHIKELQERF 360
Query: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTS 420
AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTS
Sbjct: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTS 420
Query: 421 QVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV 480
QVHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV
Sbjct: 421 QVHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFV 480
Query: 481 ACQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNF 540
ACQASKKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIGYAL+M ADERERRHHHNF
Sbjct: 481 ACQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALDMPADERERRHHHNF 540
Query: 541 MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNA 600
MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLP REAVN Y +SHNRLVILGFNA
Sbjct: 541 MHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPIREAVNCYSRSHNRLVILGFNA 600
Query: 601 TLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDY 660
TLTEAVDALGRRYGQIRDMDL+++P V+EPLKKLCDD NTTVVVLSGSDRSVLDDNFGDY
Sbjct: 601 TLTEAVDALGRRYGQIRDMDLRLNPAVKEPLKKLCDDKNTTVVVLSGSDRSVLDDNFGDY 660
Query: 661 NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQW 720
NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL W
Sbjct: 661 NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVW 720
Query: 721 NYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI 780
NYK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI
Sbjct: 721 NYKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEI 780
Query: 781 VRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPF-GVPVPKLP 840
VRDKG N+PIDYVLCVGHFLTKDEDVYTFFEPDLP+EVP T TNPF GVPVPK+P
Sbjct: 781 VRDKGINKPIDYVLCVGHFLTKDEDVYTFFEPDLPTEVPAAVARTTVTNPFGGVPVPKIP 840
Query: 841 NSGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYF 900
N+ V P S + RLKKQRSLS+LER+ANSHGSAVFWRP++QDRLSLHEGSSVLDLKGDNYF
Sbjct: 841 NTVVSP-SRSARLKKQRSLSILERKANSHGSAVFWRPVVQDRLSLHEGSSVLDLKGDNYF 900
Query: 901 SCAVGRKRSNARYLLGSSDDVVKLVKELAECS 931
SCAVGRKRSNARYL+GSSDDVV LVKEL+ECS
Sbjct: 901 SCAVGRKRSNARYLIGSSDDVVTLVKELSECS 931
BLAST of MS013523 vs. ExPASy TrEMBL
Match:
A0A5A7UR17 (Alpha,alpha-trehalose-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002680 PE=3 SV=1)
HSP 1 Score: 1710.7 bits (4429), Expect = 0.0e+00
Identity = 857/957 (89.55%), Postives = 889/957 (92.89%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLN-EEARDGYSLGEVNSSDLFTNFGG 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNRSSSLN +E+RD SLGEVNS DLFTN G
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNRSSSLNDQESRDACSLGEVNSYDLFTNAGD 60
Query: 61 SYGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEIS 120
GSF EED+LEG+SV KD D QRPEGR KQRLLVVANRLPVSAVR+GKETWHLEIS
Sbjct: 61 ICGSFNEEDVLEGISVVKDSFDGFQRPEGRTYKQRLLVVANRLPVSAVRKGKETWHLEIS 120
Query: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG
Sbjct: 121 VGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNG 180
Query: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY
Sbjct: 181 YCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDY 240
Query: 241 HLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYAR 300
HLMFLPKCLKEHN+ MKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTYDYAR
Sbjct: 241 HLMFLPKCLKEHNSTMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTYDYAR 300
Query: 301 HFVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERF 360
HFVSACTRILGLEGTPEGVEDQGKLT IAAFPIGIDS+RFIRALKLPQVQDH+KELQERF
Sbjct: 301 HFVSACTRILGLEGTPEGVEDQGKLTHIAAFPIGIDSERFIRALKLPQVQDHIKELQERF 360
Query: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDY----- 420
AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +
Sbjct: 361 AGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPECMIILS 420
Query: 421 --------------------QKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCA 480
QKLTSQVHEIVGRINGRFGTL+AVPIHHLDRSLDFHALCA
Sbjct: 421 QTYIMRNGAFKDLVFFDVTDQKLTSQVHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCA 480
Query: 481 LYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPWNIT 540
LYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGA+LVNPWNIT
Sbjct: 481 LYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAILVNPWNIT 540
Query: 541 EVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPP 600
EVAASIGYAL+M ADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPP
Sbjct: 541 EVAASIGYALDMPADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPP 600
Query: 601 LLPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLC 660
LLP REAVN Y +SHNRLVILGFNATLTEAVDALGRRYGQIRDMDL+++P V+EPLKKLC
Sbjct: 601 LLPIREAVNCYSRSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLRLNPAVKEPLKKLC 660
Query: 661 DDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESV 720
DD NTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESV
Sbjct: 661 DDKNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESV 720
Query: 721 KHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQ 780
KHVFEYFTERTPRSHFELRETSL WNYK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQ
Sbjct: 721 KHVFEYFTERTPRSHFELRETSLVWNYKYADVEFGRLQARDLLQHLWTGPISNASVDVVQ 780
Query: 781 GSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLP 840
GSRSVEVRAVGVTKGAAIDRILGEIVRDKG N+PIDYVLCVGHFLTKDEDVYTFFEPDLP
Sbjct: 781 GSRSVEVRAVGVTKGAAIDRILGEIVRDKGINKPIDYVLCVGHFLTKDEDVYTFFEPDLP 840
Query: 841 SEVPPTARATVTTNPF-GVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFW 900
+EVP T TNPF GVPVPK+PN+ V P S + RLKKQRSLS+LER+ANSHGSAVFW
Sbjct: 841 TEVPAAVARTTVTNPFGGVPVPKIPNTVVSP-SRSARLKKQRSLSILERKANSHGSAVFW 900
Query: 901 RPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECS 931
RP++QDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYL+GSSDDVV LVKEL+ECS
Sbjct: 901 RPVVQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLIGSSDDVVTLVKELSECS 956
BLAST of MS013523 vs. ExPASy TrEMBL
Match:
A0A6J1GTE0 (Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucurbita moschata OX=3662 GN=LOC111457260 PE=4 SV=1)
HSP 1 Score: 1697.6 bits (4395), Expect = 0.0e+00
Identity = 852/933 (91.32%), Postives = 879/933 (94.21%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNR SSLNEEA +SLGE NS DLF+ G S
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNR-SSLNEEA---HSLGEFNSYDLFSFSGDS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
GSF EEDILEGVSV KD D QR EGR SKQRLLVVANRLPVSAVR+GKETWHLEISV
Sbjct: 61 CGSFNEEDILEGVSVVKDIIDGFQRLEGRTSKQRLLVVANRLPVSAVRKGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDE GQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEVGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKAN+MFADVVNK+YEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANKMFADVVNKHYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLKEHN KMKVGWFLHTPFPSSEIHRTLPSR+ELLR+VLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKEHNRKMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQ+H+KELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQEHIKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTLTAVPIHHLDRSLDF ALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFPALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRH HNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHLHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDT+VEAQIRTRQIPPLLP EA N Y +SHNRLVILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTVVEAQIRTRQIPPLLPINEAANSYSRSHNRLVILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRR GQIRDMDLKVHPDVREPLKKLCDD NTTVVVLSGSDRSVLD+NFG YN
Sbjct: 601 LTEAVDALGRRCGQIRDMDLKVHPDVREPLKKLCDDRNTTVVVLSGSDRSVLDENFGQYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL WN
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVWN 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKG+AIDRILGEIV
Sbjct: 721 YKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGSAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
RDKG N+PIDYVLCVGHFL KDEDVYTFFEPDLPSEVP T P +P PK+PN+
Sbjct: 781 RDKGINKPIDYVLCVGHFLAKDEDVYTFFEPDLPSEVPQTV-------PNPLPPPKMPNN 840
Query: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
G PPKS RLKKQRSLS++ERRANSHGSAVFWRP++QDRLSLHEGSSVLDLKGDNYFSC
Sbjct: 841 GAPPKS--ARLKKQRSLSIIERRANSHGSAVFWRPVVQDRLSLHEGSSVLDLKGDNYFSC 900
Query: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
AVGRKRSNARYLL SS+DVVKLVKEL+EC SS
Sbjct: 901 AVGRKRSNARYLLVSSNDVVKLVKELSECLESS 920
BLAST of MS013523 vs. ExPASy TrEMBL
Match:
A0A6J1K5Z0 (Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucurbita maxima OX=3661 GN=LOC111490374 PE=3 SV=1)
HSP 1 Score: 1696.0 bits (4391), Expect = 0.0e+00
Identity = 851/933 (91.21%), Postives = 878/933 (94.11%), Query Frame = 0
Query: 1 MLSNNHSCSPPTPRTRLERLLRERELRRSNRSSSLNEEARDGYSLGEVNSSDLFTNFGGS 60
MLSNNH+ SPPTPRTRLERLLRERELRRSNR SSLNEEA +SLGE NS DLF+ G S
Sbjct: 1 MLSNNHNNSPPTPRTRLERLLRERELRRSNR-SSLNEEA---HSLGEFNSYDLFSFSGDS 60
Query: 61 YGSFYEEDILEGVSVAKDFSDALQRPEGRYSKQRLLVVANRLPVSAVRRGKETWHLEISV 120
GSF EEDILEGVSV KD D QR EGR SKQRLLVVANRLPVSAVR+GKETWHLEISV
Sbjct: 61 CGSFNEEDILEGVSVVKDIIDGFQRLEGRTSKQRLLVVANRLPVSAVRKGKETWHLEISV 120
Query: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
GGLVSALLGLKEFEARWIGWAGVNVPDE GQRALEKALAEKKCIPVFLDEEIVHQYYNGY
Sbjct: 121 GGLVSALLGLKEFEARWIGWAGVNVPDEVGQRALEKALAEKKCIPVFLDEEIVHQYYNGY 180
Query: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYH 240
CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKAN+MFADVVNK+YEEGDVVWCHDYH
Sbjct: 181 CNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANKMFADVVNKHYEEGDVVWCHDYH 240
Query: 241 LMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARH 300
LMFLPKCLKEHN KMKVGWFLHTPFPSSEIHRTLPSR+ELLR+VLAADLVGFHTYDYARH
Sbjct: 241 LMFLPKCLKEHNRKMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARH 300
Query: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFA 360
FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDH+KELQERFA
Sbjct: 301 FVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHIKELQERFA 360
Query: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQ 420
GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRD+VVLLQIAVPTRTDV +YQKLTSQ
Sbjct: 361 GRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDKVVLLQIAVPTRTDVPEYQKLTSQ 420
Query: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
VHEIVGRINGRFGTLTAVPIHHLDRSLDF ALCALYAVTDVALVTSLRDGMNLVSYEFVA
Sbjct: 421 VHEIVGRINGRFGTLTAVPIHHLDRSLDFPALCALYAVTDVALVTSLRDGMNLVSYEFVA 480
Query: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNFM 540
CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRH HNFM
Sbjct: 481 CQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHLHNFM 540
Query: 541 HVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNAT 600
HVTTHTSQEWAATFVSELNDT+VEAQIRTRQIPPLLP EA N Y +SHNRLVILGFNAT
Sbjct: 541 HVTTHTSQEWAATFVSELNDTVVEAQIRTRQIPPLLPINEAANSYSRSHNRLVILGFNAT 600
Query: 601 LTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYN 660
LTEAVDALGRR GQIRDMDLKVHPDVREPLKKLCDD NTTVVVLSGSDRSVLD+NFG YN
Sbjct: 601 LTEAVDALGRRCGQIRDMDLKVHPDVREPLKKLCDDRNTTVVVLSGSDRSVLDENFGQYN 660
Query: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWN 720
MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSL WN
Sbjct: 661 MWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLVWN 720
Query: 721 YKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIV 780
YK+ADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKG+AIDRILGEIV
Sbjct: 721 YKYADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGSAIDRILGEIV 780
Query: 781 RDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNS 840
RDKG N+PIDYVLCVGHFL KDEDVYTFFEPDLPSEVP T P +P PK+PN+
Sbjct: 781 RDKGINKPIDYVLCVGHFLAKDEDVYTFFEPDLPSEVPQTV-------PNPLPPPKMPNN 840
Query: 841 GVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSC 900
G PP + RLKKQRSLS++ERRANSHGSAVFWRP++QDRLSLHEGSSVLDLKGDNYFSC
Sbjct: 841 GAPPPK-SARLKKQRSLSIIERRANSHGSAVFWRPVVQDRLSLHEGSSVLDLKGDNYFSC 900
Query: 901 AVGRKRSNARYLLGSSDDVVKLVKELAECSASS 934
AVGRKRSNARYLL SS+DVVKLVKEL+EC SS
Sbjct: 901 AVGRKRSNARYLLVSSNDVVKLVKELSECLESS 921
BLAST of MS013523 vs. TAIR 10
Match:
AT1G78580.1 (trehalose-6-phosphate synthase )
HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 702/951 (73.82%), Postives = 793/951 (83.39%), Query Frame = 0
Query: 1 MLSNNHSCSPP-TPRTRLERLLREREL---RRSNRSSSLNEEARDGYSLGEVNSSDLFTN 60
M N ++CS P +R ERLLR+REL R+SNR+ + N+ A G +S+
Sbjct: 1 MPGNKYNCSSSHIPLSRTERLLRDRELREKRKSNRARNPNDVA------GSSENSENDLR 60
Query: 61 FGGSYGSFYEEDILEGVSVAKDFSDALQRPEGR-YSKQRLLVVANRLPVSAVRRGKETWH 120
G Y E LEG + A DA +R E R Y++QRLLVVANRLPVSAVRRG+++W
Sbjct: 61 LEGDSSRQYVEQYLEGAAAAMAHDDACERQEVRPYNRQRLLVVANRLPVSAVRRGEDSWS 120
Query: 121 LEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAGQRALEKALAEKKCIPVFLDEEIVHQ 180
LEIS GGLVSALLG+KEFEARWIGWAGVNVPDE GQ+AL KALAEK+CIPVFLDEEIVHQ
Sbjct: 121 LEISAGGLVSALLGVKEFEARWIGWAGVNVPDEVGQKALSKALAEKRCIPVFLDEEIVHQ 180
Query: 181 YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNKYYEEGDVVW 240
YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQF AYKKANQMFADVVN++YEEGDVVW
Sbjct: 181 YYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVW 240
Query: 241 CHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTY 300
CHDYHLMFLPKCLKE+N+KMKVGWFLHTPFPSSEIHRTLPSR+ELLRSVLAADLVGFHTY
Sbjct: 241 CHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVGFHTY 300
Query: 301 DYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKEL 360
DYARHFVSACTRILGLEGTPEGVEDQG+LTR+AAFPIGIDSDRFIRAL++P+V H+KEL
Sbjct: 301 DYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALEVPEVIQHMKEL 360
Query: 361 QERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQ 420
+ERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN WRD+VVLLQIAVPTRTDV +YQ
Sbjct: 361 KERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEENANWRDKVVLLQIAVPTRTDVPEYQ 420
Query: 421 KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS 480
KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS
Sbjct: 421 KLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVS 480
Query: 481 YEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRH 540
YEFVACQ +KKGVLILSEFAGAAQSLGAGA+LVNPWNITEVAASIG ALNM A+ERE+RH
Sbjct: 481 YEFVACQEAKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMTAEEREKRH 540
Query: 541 HHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQIPPLLPTREAVNRYVQSHNRLVIL 600
HNF HV THT+QEWA TFVSELNDT++EAQ+R ++PP LP +A+ RY +S+NRL+IL
Sbjct: 541 RHNFHHVKTHTAQEWAETFVSELNDTVIEAQLRISKVPPELPQHDAIQRYSKSNNRLLIL 600
Query: 601 GFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDN 660
GFNATLTE VD GRR QI++MDL +HP+++ PLK LC DP+TT+VVLSGS RSVLD N
Sbjct: 601 GFNATLTEPVDNQGRRGDQIKEMDLNLHPELKGPLKALCSDPSTTIVVLSGSSRSVLDKN 660
Query: 661 FGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFTERTPRSHFELRET 720
FG+Y+MWLAAENGMFLR T GEWMTTMPE+L+M+WV+SVKHVF+YFTERTPRSHFE R+T
Sbjct: 661 FGEYDMWLAAENGMFLRLTNGEWMTTMPEHLNMEWVDSVKHVFKYFTERTPRSHFETRDT 720
Query: 721 SLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI 780
SL WNYK+AD+EFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI
Sbjct: 721 SLIWNYKYADIEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRI 780
Query: 781 LGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVP 840
LGEIV K PIDYVLC+GHFL KDEDVYTFFEP+LPS++P AR+ +++
Sbjct: 781 LGEIVHSKSMTTPIDYVLCIGHFLGKDEDVYTFFEPELPSDMPAIARSRPSSDS---GAK 840
Query: 841 KLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSAVFWRPIMQDRLSLHEGS-------- 900
PP +T K S S +N++ + R + +R S S
Sbjct: 841 SSSGDRRPPSKSTHNNNKSGSKSSSSSNSNNNNKS-SQRSLQSERKSGSNHSLGNSRRPS 900
Query: 901 ------SVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECSAS 933
+VLDLKG+NYFSCAVGR R+NARYLLGS DDVV +++LA+ ++S
Sbjct: 901 PEKISWNVLDLKGENYFSCAVGRTRTNARYLLGSPDDVVCFLEKLADTTSS 941
BLAST of MS013523 vs. TAIR 10
Match:
AT1G16980.1 (trehalose-phosphatase/synthase 2 )
HSP 1 Score: 1019.2 bits (2634), Expect = 2.1e-297
Identity = 498/842 (59.14%), Postives = 636/842 (75.53%), Query Frame = 0
Query: 87 EGRYSKQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGL-KEFEARWIGWAGVNV 146
+ R + RLLVVANRLPVSA R G+ +W LE+S GGLVS LLG+ +F+ +W+GW GV+V
Sbjct: 5 DARGERPRLLVVANRLPVSAKRTGENSWSLEMSPGGLVSGLLGITSQFDTKWVGWPGVDV 64
Query: 147 PDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRS 206
DE + AL ++LAE KCIPVFL+ + QYYNGYCN ILWP+ H++GLPQED+ T ++
Sbjct: 65 HDEIEKNALTESLAEMKCIPVFLN-GVFDQYYNGYCNGILWPILHHMGLPQEDQHDTNQT 124
Query: 207 FQSQFDAYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPF 266
F++Q+DAYKKAN+MF DV+ YEEGD+VWCHDYHLMFLP+ LKE+NNK+KVGWFLH+PF
Sbjct: 125 FETQYDAYKKANRMFLDVIIDNYEEGDIVWCHDYHLMFLPQYLKEYNNKIKVGWFLHSPF 184
Query: 267 PSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLT 326
PSSE+++TLPSR+ELLR++LAADL+GFHTYD+ARHF+S CTRILG+EGT EGV QG++T
Sbjct: 185 PSSEVYKTLPSRSELLRAILAADLLGFHTYDFARHFLSTCTRILGVEGTHEGVVYQGRVT 244
Query: 327 RIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFE 386
R+A FPIGID DRFIR KLP+V + ELQE+FAG+KV+LGVDRLDMIKGIPQK LAFE
Sbjct: 245 RVAVFPIGIDPDRFIRTCKLPEVTQQMNELQEKFAGKKVILGVDRLDMIKGIPQKYLAFE 304
Query: 387 KFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDR 446
KFLEENP WRD+VVL+QIAVPTR DV +Y+KL SQVH +VGRINGRFG+++++PIHHLD
Sbjct: 305 KFLEENPYWRDKVVLVQIAVPTRNDVPEYRKLKSQVHGLVGRINGRFGSVSSLPIHHLDC 364
Query: 447 SLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGA 506
S+DF+ LCA+YA+ DV LVTSLRDGMNLVSYEFVACQ +KKGVL+LSEFAGA QSLG GA
Sbjct: 365 SVDFNYLCAIYAIADVMLVTSLRDGMNLVSYEFVACQEAKKGVLVLSEFAGAGQSLGVGA 424
Query: 507 VLVNPWNITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEA 566
++VNPW++TEV+++I ALNM A+ERE RH NF +V TH++++W F+SELN I E+
Sbjct: 425 LIVNPWDVTEVSSAIKEALNMPAEERETRHRSNFQYVCTHSAEKWGLDFMSELNGIIPES 484
Query: 567 QIRTRQIPPLLPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPD 626
+++ R+IP LP ++ + +Y QS+NRL+ILGF TL E +++ ++MDLK++P+
Sbjct: 485 EMQMRKIPLQLPEQDVIQQYSQSNNRLIILGFFGTLAEPMNS------GTKEMDLKLNPE 544
Query: 627 VREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPEN 686
++ LK LC+DP TTVVVLS S +++L+ NFG+ N+WLAAENGMF + T GEW+T MP+N
Sbjct: 545 LKGTLKALCNDPKTTVVVLSRSGKNILNKNFGESNIWLAAENGMFEKQTTGEWVTNMPQN 604
Query: 687 LHMDWVESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPI 746
+++DWV+ VK+VF+YFT+RTPRS+FE ETSL WNY++ADVEFGR QARDLLQ+LW GPI
Sbjct: 605 VNLDWVDGVKNVFKYFTDRTPRSYFEASETSLVWNYEYADVEFGRAQARDLLQYLWAGPI 664
Query: 747 SNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDV 806
SNASVDVV+G+ SVEV A+G TKGAAI RILGEIV K PID+V C G+FL KDED+
Sbjct: 665 SNASVDVVRGNHSVEVHAIGETKGAAIGRILGEIVHRKSMTTPIDFVFCSGYFLEKDEDI 724
Query: 807 YTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRAN 866
YTFFE + S K PN
Sbjct: 725 YTFFESKILSS-------------------KSPNG------------------------- 771
Query: 867 SHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKE 926
LDLK +NYFS A+G+ R+ ARY++ S+ VV L+ +
Sbjct: 785 ------------------------LDLKKENYFSAAIGQARTKARYVIDSAHGVVDLLHK 771
Query: 927 LA 928
LA
Sbjct: 845 LA 771
BLAST of MS013523 vs. TAIR 10
Match:
AT4G27550.1 (trehalose-6-phosphatase synthase S4 )
HSP 1 Score: 989.2 bits (2556), Expect = 2.3e-288
Identity = 498/846 (58.87%), Postives = 627/846 (74.11%), Query Frame = 0
Query: 91 SKQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAG 150
++ RLLVV+ LPV+A R G+E+W +S GGLVSALLGLKEFE +WIGW GV+V D G
Sbjct: 2 ARPRLLVVSMSLPVTAKRTGEESWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAIG 61
Query: 151 QRALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQF 210
++ L LAEK CIPVFL EE+ QYYNGYCNNILWP+FHYLG P E R T ++QSQ+
Sbjct: 62 KKTLSITLAEKGCIPVFL-EEVCDQYYNGYCNNILWPIFHYLGTPPEYRNDATITYQSQY 121
Query: 211 DAYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEI 270
+AYKKANQ+F DVV ++YEEGDVVWCHDYH+M LP+ LKE+N+KMKVGWFLHTPFPSSE+
Sbjct: 122 EAYKKANQIFFDVVKEHYEEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEM 181
Query: 271 HRTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAF 330
++TLPSR++LLRSVL ADLVGFHTYD+ARHF++AC ILG+E T EG+ DQGK+TR+A F
Sbjct: 182 YKTLPSRSDLLRSVLTADLVGFHTYDFARHFLNACMCILGVEATSEGIVDQGKVTRVAVF 241
Query: 331 PIGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE 390
PIGI+ +RFI +L +V ++K+ + F GRK++LGVDRLD IKGIPQK AFEKFLEE
Sbjct: 242 PIGIEPERFINTSELSEVVQYMKKFKNDFGGRKLILGVDRLDTIKGIPQKYQAFEKFLEE 301
Query: 391 NPKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFH 450
N +WR +V+LLQIAVPTR + +YQK+ Q H VGRINGRFG++++VPI HLD S+DF+
Sbjct: 302 NAEWRGKVMLLQIAVPTRNGIGEYQKIKDQCHYHVGRINGRFGSISSVPIIHLDCSIDFN 361
Query: 451 ALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNP 510
LCALYA+TDV LVTSLRDGMNLVS EF+ACQ ++KGVLILSEFAGA QSLGAGA+LVNP
Sbjct: 362 QLCALYAITDVLLVTSLRDGMNLVSSEFIACQKAEKGVLILSEFAGAGQSLGAGAILVNP 421
Query: 511 WNITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFV---------SELNDT 570
WNI EV+++IG ALNM +E+ER+H NF +V TH++Q+WA F+ S+L +
Sbjct: 422 WNIKEVSSAIGEALNMSHEEKERKHKINFQYVKTHSTQQWADDFMKLTLTNILCSKLIEI 481
Query: 571 IVEAQIRTRQIPPL-LPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDL 630
A++ L LP + + +Y +S+NRL+ILGF TLT+ + RR M+L
Sbjct: 482 TTSAELGAGLAATLELPEHDVIQQYSKSNNRLLILGFYGTLTQPMKNQERRGD---GMNL 541
Query: 631 KVHPDVREPLKKLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMT 690
++HP ++E LK+LC DP TTVVVLS S++ +LD NFG+YNMWLAAENGMFLR T GEW+T
Sbjct: 542 ELHPQLKERLKELCSDPKTTVVVLSRSEKCILDKNFGEYNMWLAAENGMFLRHTSGEWVT 601
Query: 691 TMPENLHMDWVESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHL 750
+PE+++++W++ VKHVF+YFTERTP S+ E E SL WNY+ AD EFGR QARD+LQHL
Sbjct: 602 RIPEHMNLEWIDGVKHVFKYFTERTPGSYLETSEASLVWNYENADAEFGRAQARDMLQHL 661
Query: 751 WTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLT 810
W GPISNASVDVV+G +SVEV AVGVTKG+A++RILGEIV +K PIDYVLC+G FL
Sbjct: 662 WAGPISNASVDVVRGGQSVEVHAVGVTKGSAMERILGEIVHNKSMATPIDYVLCIGCFLG 721
Query: 811 KDEDVYTFFEPDLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVL 870
KDEDVYTFFEP+L KK +SLS
Sbjct: 722 KDEDVYTFFEPEL-------------------------------------TKKAKSLS-- 781
Query: 871 ERRANSHGSAVFWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVV 927
S GS ++S S+++DLKG+NYFS A+G+ + ARY L SSDDVV
Sbjct: 782 -----SSGSD------SPKKVS----STIVDLKGENYFSVAIGQTHTKARYFLDSSDDVV 789
BLAST of MS013523 vs. TAIR 10
Match:
AT1G17000.1 (trehalose-phosphatase/synthase 3 )
HSP 1 Score: 948.7 bits (2451), Expect = 3.4e-276
Identity = 472/842 (56.06%), Postives = 619/842 (73.52%), Query Frame = 0
Query: 92 KQRLLVVANRLPVSAVRRGKETWHLEISVGGLVSALLGLKEFEARWIGWAGVNVPDEAGQ 151
+Q LLVVANRLP SA R G+ +W LE+S GG + L+ +
Sbjct: 10 RQTLLVVANRLPASAKRTGEHSWSLEMSPGGKFNLLV---------------------EK 69
Query: 152 RALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFD 211
A+ K+LAE KCIPVFL+ E+ QYYNGY N ILWP+ H++GLPQE T ++F++Q+D
Sbjct: 70 DAVSKSLAEMKCIPVFLN-EVFDQYYNGYSNGILWPILHHMGLPQEYDHDTIKTFETQYD 129
Query: 212 AYKKANQMFADVVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVGWFLHTPFPSSEIH 271
AYKKAN+MF DV+ + Y++GD+VWC DYHLMFLP+ LKE+NNK+KVGWFLH+PFPSSEI+
Sbjct: 130 AYKKANRMFLDVIKENYKDGDIVWCQDYHLMFLPQYLKEYNNKIKVGWFLHSPFPSSEIY 189
Query: 272 RTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRIAAFP 331
+TLPSR+ELLRSVLAADL+ FHTYD+ARHFV+ CTRILG+EGT EGV QG++TR+ P
Sbjct: 190 KTLPSRSELLRSVLAADLISFHTYDFARHFVNTCTRILGVEGTHEGVVYQGRVTRVVVLP 249
Query: 332 IGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN 391
+GI +RFI+ KLP+V + EL++RF+G+KV+LGVDRLDMIKGIPQK L FEKFL+EN
Sbjct: 250 MGIYPNRFIKTCKLPEVIQQMNELKDRFSGKKVILGVDRLDMIKGIPQKYLGFEKFLDEN 309
Query: 392 PKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHA 451
P WRD++VL+QIAVPTR +V +YQKL +QVH +VGRINGRFG+++++PIHH+D S+D +
Sbjct: 310 PNWRDKIVLVQIAVPTRNEVPEYQKLKNQVHRLVGRINGRFGSVSSLPIHHMDCSVDSNY 369
Query: 452 LCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAVLVNPW 511
LCALYA++DV LVTSLRDG+NLVS+EFVACQ +K+GVLILSEFAGA QSLGAGA+LVNPW
Sbjct: 370 LCALYAISDVMLVTSLRDGLNLVSHEFVACQEAKRGVLILSEFAGAGQSLGAGALLVNPW 429
Query: 512 NITEVAASIGYALNMEADERERRHHHNFMHVTTHTSQEWAATFVSELNDTIVEAQIRTRQ 571
N+TEV+++I ALNM +ERE RH NF +V TH++++W F+SELND E++++ R+
Sbjct: 430 NVTEVSSAIKKALNMPYEERETRHRVNFKYVKTHSAEKWGFDFLSELNDAFDESELQIRK 489
Query: 572 IPPLLPTREAVNRYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLK 631
IP LP ++ + RY S+NRL+ILGF T+TE ++L ++MDL ++P+++E LK
Sbjct: 490 IPHELPQQDVIQRYSLSNNRLIILGFYGTITEPRNSLS------KEMDLXLNPELKETLK 549
Query: 632 KLCDDPNTTVVVLSGSDRSVLDDNFGDYNMWLAAENGMFLRPTKGEWMTTMPENLHMDWV 691
LC+DP TTVVVLS S +++LD NFG+Y +WLAAENGMFL+ T EW+T MP+N+++DWV
Sbjct: 550 ALCNDPKTTVVVLSRSGKNILDKNFGEYKIWLAAENGMFLKHTTEEWVTNMPQNMNLDWV 609
Query: 692 ESVKHVFEYFTERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVD 751
+ +K+VF+YFT+RTPRS FE +TSL WNY++ADVEFGR QARDLLQ+LW GPISNAS +
Sbjct: 610 DGLKNVFKYFTDRTPRSFFEASKTSLVWNYEYADVEFGRAQARDLLQYLWAGPISNASAE 669
Query: 752 VVQGSRSVEVRAVGVTKGAAIDRILGEIVRDKGTNQPIDYVLCVGHFLTKDEDVYTFFEP 811
VV+G SVEV A+GVTK I ILGEIV K PIDYV C G+FL KDED+YTFFE
Sbjct: 670 VVRGKYSVEVHAIGVTKEPEIGHILGEIVHKKAMTTPIDYVFCSGYFLEKDEDIYTFFES 729
Query: 812 DLPSEVPPTARATVTTNPFGVPVPKLPNSGVPPKSTTTRLKKQRSLSVLERRANSHGSAV 871
++ S PKL S TR K S LE++ +
Sbjct: 730 EILS-------------------PKL--------SHETRSKSSSSNHSLEKKVS------ 775
Query: 872 FWRPIMQDRLSLHEGSSVLDLKGDNYFSCAVGRKRSNARYLLGSSDDVVKLVKELAECSA 931
+VLDLK +NYFS A+G+ R+ ARY++ SS +VV L+ +LA +
Sbjct: 790 ---------------LNVLDLKQENYFSTAIGQARTKARYVVDSSHNVVNLLHKLAVANT 775
Query: 932 SS 934
++
Sbjct: 850 TT 775
BLAST of MS013523 vs. TAIR 10
Match:
AT4G17770.1 (trehalose phosphatase/synthase 5 )
HSP 1 Score: 459.1 bits (1180), Expect = 8.2e-129
Identity = 282/764 (36.91%), Postives = 427/764 (55.89%), Query Frame = 0
Query: 91 SKQRLLVVANRLPVSAVRR--GKETWHLEISVGGLVSALLGLK-----EFEARWIGWAGV 150
++ R+++V N+LP+ + R GK ++ + S LL LK + E +IG
Sbjct: 58 TQDRIIIVGNQLPIKSHRNSAGKLSFSWDND-----SLLLQLKDGMREDMEVVYIGCLKE 117
Query: 151 NV----PDEAGQRALEKALAEKKCIPVFLDEEIVHQYYNGYCNNILWPLFHYLGLPQEDR 210
+ D+ QR LE KC+P ++ E+ +YY+G+C LWPLFHY+ LP
Sbjct: 118 QIDTVEQDDVSQRLLENF----KCVPAYIPPELFTKYYHGFCKQHLWPLFHYM-LPLTPD 177
Query: 211 LATTRSFQSQFDAYKKANQMFAD-VVNKYYEEGDVVWCHDYHLMFLPKCLKEHNNKMKVG 270
L R +S + AY N++FAD V+ + D VW HDYHLM LP L++ N++K+G
Sbjct: 178 LG-GRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKRFNRVKLG 237
Query: 271 WFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFHTYDYARHFVSACTRILGLEGTPE-- 330
+FLH+PFPSSEI+RTLP R ELLR++L ADL+GFHT+DYARHF+S C+R+LGL +
Sbjct: 238 FFLHSPFPSSEIYRTLPVRNELLRALLNADLIGFHTFDYARHFLSCCSRMLGLSYQSKRG 297
Query: 331 --GVEDQGKLTRIAAFPIGIDSDRFIRALKLPQVQDHVKELQERFAGRKVMLGVDRLDMI 390
G+E G+ I P+GI + L LP+ Q V EL+++F +KV+LGVD +D+
Sbjct: 298 TIGLEYYGRTVSIKILPVGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIF 357
Query: 391 KGIPQKILAFEKFLEENPKWRDRVVLLQIAVPTRTDVRDYQKLTSQVHEIVGRINGRFGT 450
KGI K+LA E+ L ++P+ R RVVL+QIA P R +D Q++ S+ V RIN FG
Sbjct: 358 KGISLKLLAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATVKRINEMFGR 417
Query: 451 LTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQ------------ 510
P+ +D L F A Y + + LVT++RDGMNL+ YE++ C+
Sbjct: 418 PGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIPYEYIICRQGNPKLNETIGL 477
Query: 511 ---ASKKGVLILSEFAGAAQSLGAGAVLVNPWNITEVAASIGYALNMEADERERRHHHNF 570
A+KK +L++SEF G + SL +GA+ VNPWNI V ++ YAL + E++ RH +
Sbjct: 478 DPSAAKKSMLVVSEFIGCSPSL-SGAIRVNPWNIDAVTEAMDYALIVSEAEKQMRHEKHH 537
Query: 571 MHVTTHTSQEWAATFVSELNDTIVEAQIRTR----------QIPPLLPT------REAVN 630
+V+TH WA +F+ +L + +R R ++ L P+ V+
Sbjct: 538 KYVSTHDVAYWARSFIQDLERACGD-HVRKRCWGIGFGLGFRVVALDPSFKKLSIEHIVS 597
Query: 631 RYVQSHNRLVILGFNATLTEAVDALGRRYGQIRDMDLKVHPDVREPLKKLCDDPNTTVVV 690
Y ++ NR ++L ++ T+ + G IR + + E L L DP V +
Sbjct: 598 AYKRTKNRAILLDYDGTMVQP--------GSIRTTPTR---ETIEILNNLSSDPKNIVYL 657
Query: 691 LSGSDRSVLDDNFGDY-NMWLAAENGMFLRPTKGEWMTTMPENLHMDWVESVKHVFEYFT 750
+SG DR L + F ++ L AE+G F+RP G T +W + + V +T
Sbjct: 658 VSGKDRRTLTEWFSSCDDLGLGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYT 717
Query: 751 ERTPRSHFELRETSLQWNYKFADVEFGRLQARDLLQHLWTGPISNASVDVVQGSRSVEVR 807
E T S E +ET+L WNY+FAD +FG QA++L++HL ++N V V G + VEV+
Sbjct: 718 ETTDGSTIETKETALVWNYQFADPDFGSCQAKELMEHL-ESVLTNDPVSVKTGQQLVEVK 777
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022139989.1 | 0.0e+00 | 100.00 | alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like [Momordica charant... | [more] |
XP_038894955.1 | 0.0e+00 | 92.72 | alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like isoform X2 [Beninc... | [more] |
XP_031736267.1 | 0.0e+00 | 91.97 | alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 [Cucumis sativus] >KGN6... | [more] |
XP_008443729.1 | 0.0e+00 | 92.06 | PREDICTED: alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like [Cucumi... | [more] |
XP_038894954.1 | 0.0e+00 | 89.93 | alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like isoform X1 [Beninc... | [more] |
Match Name | E-value | Identity | Description | |
Q9SYM4 | 0.0e+00 | 73.82 | Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Arabidopsis thaliana... | [more] |
Q9FZ57 | 2.9e-296 | 59.14 | Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 OS=Arabidopsis... | [more] |
Q9T079 | 3.2e-287 | 58.87 | Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4 OS=Arabidopsis... | [more] |
Q9SHG0 | 9.7e-276 | 56.18 | Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 3 OS=Arabidopsis... | [more] |
Q54K57 | 1.7e-163 | 41.94 | Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoi... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CEH2 | 0.0e+00 | 100.00 | Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Momordica charantia OX... | [more] |
A0A1S3B8S6 | 0.0e+00 | 92.06 | Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucumis melo OX=3656 G... | [more] |
A0A5A7UR17 | 0.0e+00 | 89.55 | Alpha,alpha-trehalose-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 ... | [more] |
A0A6J1GTE0 | 0.0e+00 | 91.32 | Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucurbita moschata OX=... | [more] |
A0A6J1K5Z0 | 0.0e+00 | 91.21 | Alpha,alpha-trehalose-phosphate synthase (UDP-forming) OS=Cucurbita maxima OX=36... | [more] |