MS013478 (gene) Bitter gourd (TR) v1

BLAST of MS013478 vs. NCBI nr
Match: XP_022139981.1 (cullin-4 [Momordica charantia])

HSP 1 Score: 1571.2 bits (4067), Expect = 0.0e+00
Identity = 818/860 (95.12%), Postives = 818/860 (95.12%), Query Frame = 0

Query: 1   MSLPTKRSASTATAAASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60
           MSLPTKRSASTATA ASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD
Sbjct: 1   MSLPTKRSASTATATASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60

Query: 61  FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKKLVIKLLKGLFFRSHSAF 120
           FDPSSMALDEDLKPDDSPLIG SRAVATNLSRKKATPPQPAKKLVIKLLK          
Sbjct: 61  FDPSSMALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLK---------- 120

Query: 121 LFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDTWAKLKSAICAIFLKQPN 180
                                         AKPTLPANFEEDTWAKLKSAICAIFLKQPN
Sbjct: 121 ------------------------------AKPTLPANFEEDTWAKLKSAICAIFLKQPN 180

Query: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240
           SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW
Sbjct: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240

Query: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300
           QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM
Sbjct: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300

Query: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360
           IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY
Sbjct: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360

Query: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420
           LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL
Sbjct: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420

Query: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480
           QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF
Sbjct: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480

Query: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540
           SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF
Sbjct: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540

Query: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600
           RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL
Sbjct: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600

Query: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660
           SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL
Sbjct: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660

Query: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720
           SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI
Sbjct: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720

Query: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780
           EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE
Sbjct: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780

Query: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 840
           ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL
Sbjct: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 820

Query: 841 IDREYLERDKNNPQIYNYLA 861
           IDREYLERDKNNPQIYNYLA
Sbjct: 841 IDREYLERDKNNPQIYNYLA 820

BLAST of MS013478 vs. NCBI nr
Match: XP_023519705.1 (cullin-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1484.2 bits (3841), Expect = 0.0e+00
Identity = 789/877 (89.97%), Postives = 801/877 (91.33%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSSA-TSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSAS TA  AASSVVSS+ TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSASATANTAASSVVSSSPTSKASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPFSNITSSAAADDADFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. NCBI nr
Match: KAG6583898.1 (Cullin-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1483.8 bits (3840), Expect = 0.0e+00
Identity = 789/877 (89.97%), Postives = 800/877 (91.22%), Query Frame = 0

Query: 1   MSLPTKRSA-STATAAASSVVSS-ATSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSA +TA  AASSVVSS  TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSATATANTAASSVVSSLPTSNASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPFSNITSSAAADDADFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. NCBI nr
Match: XP_022927143.1 (cullin-4-like [Cucurbita moschata])

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 788/877 (89.85%), Postives = 800/877 (91.22%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSS-ATSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSAS TA  AASSVVSS  TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSASATANTAASSVVSSLPTSNASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPCSNITSSAAADDADFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASID+EKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDSEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. NCBI nr
Match: XP_023001393.1 (cullin-4-like [Cucurbita maxima])

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 787/877 (89.74%), Postives = 800/877 (91.22%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSSA-TSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSAS TA  AASSVVSS+ TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSASATANTAASSVVSSSPTSNASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D +FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPFSNITSSAAADDAEFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHIS ALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISVALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. ExPASy Swiss-Prot
Match: Q8LGH4 (Cullin-4 OS=Arabidopsis thaliana OX=3702 GN=CUL4 PE=1 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 659/860 (76.63%), Postives = 703/860 (81.74%), Query Frame = 0

Query: 1   MSLPTKRSASTATAAASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60
           MSLPTKR  ST +AA++S  SS +     SPPMKK               KN LHH    
Sbjct: 1   MSLPTKR--STFSAASASDDSSYS-----SPPMKKA--------------KNDLHHSPQH 60

Query: 61  FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKKLVIKLLKGLFFRSHSAF 120
            + +   +   ++ D +P        A NLSRKKAT PQP KK VIKL K          
Sbjct: 61  PNTADKVVGFHMEEDPTP-------AAANLSRKKATLPQPTKKFVIKLNK---------- 120

Query: 121 LFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDTWAKLKSAICAIFLKQPN 180
                                         AKPTLP NFEE+TW KL+SAI AIFLK+  
Sbjct: 121 ------------------------------AKPTLPTNFEENTWEKLQSAIRAIFLKKKI 180

Query: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240
           S DLE LYQAV++LCLHK+ G LY +IEKECE HISAALQSLVGQ+ DL VFL  VEKCW
Sbjct: 181 SFDLESLYQAVDNLCLHKLDGKLYDQIEKECEEHISAALQSLVGQNTDLTVFLSRVEKCW 240

Query: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300
           QDFCDQMLMIR IAL LDR YV Q P+V SLW+MGLQLFRKHLSL+ EVE +TV GLL M
Sbjct: 241 QDFCDQMLMIRSIALTLDRKYVIQNPNVRSLWEMGLQLFRKHLSLAPEVEQRTVKGLLSM 300

Query: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360
           IEKERL EAVNRTLL+HLLKMFTALGIY ESFEKPFLE TSEFYAAEGMKYMQ SDV EY
Sbjct: 301 IEKERLAEAVNRTLLSHLLKMFTALGIYMESFEKPFLEGTSEFYAAEGMKYMQQSDVPEY 360

Query: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420
           LKH E RL EENERC+LY+D  TRKPLI T ERQLLERHI  +L+KGFT LMDG R EDL
Sbjct: 361 LKHVEGRLHEENERCILYIDAVTRKPLITTVERQLLERHILVVLEKGFTTLMDGRRTEDL 420

Query: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480
           QRM TL SRVNALESLRQALSSY+R+TGQ IVMD+EKDKDMV SLL+FKASLD IWEESF
Sbjct: 421 QRMQTLFSRVNALESLRQALSSYVRKTGQKIVMDEEKDKDMVQSLLDFKASLDIIWEESF 480

Query: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540
            KNE+F NTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELE  L+KVLVLF
Sbjct: 481 YKNESFGNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELESVLEKVLVLF 540

Query: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600
           RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL
Sbjct: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600

Query: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660
           SKEINESFKQSSQARTKLP GIEMSVHVLTTGYWPTYPPMDV+LPHELNVYQDIFKEFYL
Sbjct: 601 SKEINESFKQSSQARTKLPSGIEMSVHVLTTGYWPTYPPMDVKLPHELNVYQDIFKEFYL 660

Query: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720
           SKYSGRRLMW NSLGHCVLKA+F KGKKELAVSLFQ VVLMLFNDA KLSF+DI++ST I
Sbjct: 661 SKYSGRRLMWQNSLGHCVLKADFSKGKKELAVSLFQAVVLMLFNDAMKLSFEDIKDSTSI 720

Query: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780
           EDKELRRTLQSLACGKVRVLQK PKGRDVED D F FN+ F APLYR+KVNAIQMKETVE
Sbjct: 721 EDKELRRTLQSLACGKVRVLQKNPKGRDVEDGDEFEFNDEFAAPLYRIKVNAIQMKETVE 780

Query: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 840
           ENTSTTERVFQDRQYQ+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL
Sbjct: 781 ENTSTTERVFQDRQYQIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 792

Query: 841 IDREYLERDKNNPQIYNYLA 861
           IDREYLER+K+NPQIYNYLA
Sbjct: 841 IDREYLEREKSNPQIYNYLA 792

BLAST of MS013478 vs. ExPASy Swiss-Prot
Match: Q13620 (Cullin-4B OS=Homo sapiens OX=9606 GN=CUL4B PE=1 SV=4)

HSP 1 Score: 837.4 bits (2162), Expect = 1.4e-241
Identity = 420/713 (58.91%), Postives = 540/713 (75.74%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           ++ KP LP N+ ++TW KLK A+ AI        +LE+LYQAV +LC +K+  NLY+++ 
Sbjct: 202 FKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLR 261

Query: 209 KECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSV 268
           + CE HI A +      S D V+FLK +++CWQ+ C QM+MIR I L+LDRTYV Q   +
Sbjct: 262 QICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSML 321

Query: 269 CSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIY 328
            S+WDMGL+LFR H+    +V++KT+ G+L +IE+ER GEA++R+LL  LL M + L IY
Sbjct: 322 PSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIY 381

Query: 329 SESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLI 388
            +SFE+ FLE T+  YAAEG K MQ  +V EYL H  +RL+EE +R + YLD +T+K LI
Sbjct: 382 QDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLI 441

Query: 389 ATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRT 448
           AT E+QLL  H++AIL KG   L+D NR++DL  +Y L SRV   ++ L Q    YI+  
Sbjct: 442 ATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAF 501

Query: 449 GQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAE 508
           G  IV++ EKDK MV  LL+FK  +D I +  F KNE F N +K+AFE  IN R N+PAE
Sbjct: 502 GSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAE 561

Query: 509 LIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSA 568
           LIAK++D KLRAGNK  ++EELE  LDK++++FRFI GKDVFEAFYKKDLAKRLL+GKSA
Sbjct: 562 LIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSA 621

Query: 569 SIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVH 628
           S+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I   FKQ  Q    +P  IE++V+
Sbjct: 622 SVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQ-NQNVPGNIELTVN 681

Query: 629 VLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGK 688
           +LT GYWPTY PM+V LP E+   Q+IFK FYL K+SGR+L W ++LGHCVLKAEF +GK
Sbjct: 682 ILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGK 741

Query: 689 KELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGR 748
           KEL VSLFQT+VL++FN+ E+ S ++I+++TGIED ELRRTLQSLACGK RVL K PKG+
Sbjct: 742 KELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGK 801

Query: 749 DVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKT 808
           D+ED D F+ N+ F   L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK 
Sbjct: 802 DIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKM 861

Query: 809 RKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           RK LSH LL++E++ QLKFP+KPADLKKRIESLIDR+Y+ERDK NP  YNY+A
Sbjct: 862 RKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA 913

BLAST of MS013478 vs. ExPASy Swiss-Prot
Match: A2A432 (Cullin-4B OS=Mus musculus OX=10090 GN=Cul4b PE=1 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 2.5e-241
Identity = 419/713 (58.77%), Postives = 538/713 (75.46%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           ++ KP LP N+ ++TW KLK A+ AI        +LE+LYQAV +LC HK+  NLY+++ 
Sbjct: 259 FKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSHKISANLYKQLR 318

Query: 209 KECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSV 268
           + CE HI A +      S D V+FLK +++CWQ+ C QM+MIR I L+LDRTYV Q   +
Sbjct: 319 QICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSML 378

Query: 269 CSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIY 328
            S+WDMGL+LFR H+    +V+ KT+ G+L +IE+ER GEA++R+LL  LL M + L IY
Sbjct: 379 PSIWDMGLELFRAHIISDQKVQTKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIY 438

Query: 329 SESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLI 388
            +SFE+ FL+ T+  YAAEG K MQ  +V EYL H  +RL+EE +R + YLD +T+K LI
Sbjct: 439 QDSFEQQFLQETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLI 498

Query: 389 ATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRT 448
           A+ E+QLL  H++AIL KG   L+D NR++DL  +Y L SRV   ++ L Q    YI+  
Sbjct: 499 ASVEKQLLGEHLTAILQKGLNSLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAF 558

Query: 449 GQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAE 508
           G  IV++ EKDK MV  LL+FK  +D I +  F KNE F N +K+AFE  IN R N+PAE
Sbjct: 559 GSTIVINPEKDKTMVQELLDFKDKVDHIIDTCFLKNEKFINAMKEAFETFINKRPNKPAE 618

Query: 509 LIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSA 568
           LIAK++D KLRAGNK  ++EELE  LDK++++FRFI GKDVFEAFYKKDLAKRLL+GKSA
Sbjct: 619 LIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSA 678

Query: 569 SIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVH 628
           S+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I   FKQ  Q    +P  IE++V+
Sbjct: 679 SVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQ-NQNVPGNIELTVN 738

Query: 629 VLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGK 688
           +LT GYWPTY PM+V LP E+   Q+IFK FYL K+SGR+L W ++LGHCVLKAEF +GK
Sbjct: 739 ILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGK 798

Query: 689 KELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGR 748
           KEL VSLFQT+VL++FN+ E+ S ++I+ +TGIED ELRRTLQSLACGK RVL K PKG+
Sbjct: 799 KELQVSLFQTMVLLMFNEGEEFSLEEIKHATGIEDGELRRTLQSLACGKARVLAKNPKGK 858

Query: 749 DVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKT 808
           D+ED D F+ N+ F   L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK 
Sbjct: 859 DIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKM 918

Query: 809 RKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           RK LSH LL++E++ QLKFP+KPADLKKRIESLIDR+Y+ERDK NP  YNY+A
Sbjct: 919 RKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA 970

BLAST of MS013478 vs. ExPASy Swiss-Prot
Match: Q13619 (Cullin-4A OS=Homo sapiens OX=9606 GN=CUL4A PE=1 SV=3)

HSP 1 Score: 817.8 bits (2111), Expect = 1.2e-235
Identity = 415/713 (58.20%), Postives = 529/713 (74.19%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           +  +P LP N+ +DTW KL  A+ A+        +LE+LYQAV +LC HK+   LY+++ 
Sbjct: 48  FRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLR 107

Query: 209 KECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSV 268
           + CE H+ A +      S D V+FLK +  CWQD C QM+MIR I L+LDRTYV Q  ++
Sbjct: 108 QACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTL 167

Query: 269 CSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIY 328
            S+WDMGL+LFR H+     V+ KT+ G+L +IE+ER GEAV+R+LL  LL M + L +Y
Sbjct: 168 PSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVY 227

Query: 329 SESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLI 388
            +SFE  FLE T+  YAAEG + MQ  +V EYL H  +RL+EE +R + YLD ST+KPLI
Sbjct: 228 KDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLI 287

Query: 389 ATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRT 448
           A  E+QLL  H++AIL KG   L+D NR+ DL +MY L SRV    ++L Q  S YI+  
Sbjct: 288 ACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTF 347

Query: 449 GQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAE 508
           G  IV++ EKDKDMV  LL+FK  +D + E  F KNE F N +K++FE  IN R N+PAE
Sbjct: 348 GTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAE 407

Query: 509 LIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSA 568
           LIAK +D KLRAGNK  ++EELE TLDK+++LFRFI GKDVFEAFYKKDLAKRLL+GKSA
Sbjct: 408 LIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSA 467

Query: 569 SIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVH 628
           S+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I   FKQ  Q ++     I+++V+
Sbjct: 468 SVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSD-SGPIDLTVN 527

Query: 629 VLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGK 688
           +LT GYWPTY PM+V L  E+   Q++FK FYL K+SGR+L W  +LGH VLKAEF +GK
Sbjct: 528 ILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGK 587

Query: 689 KELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGR 748
           KE  VSLFQT+VL++FN+ +  SF++I+ +TGIED ELRRTLQSLACGK RVL K PKG+
Sbjct: 588 KEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGK 647

Query: 749 DVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKT 808
           +VED D F+FN  F   L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK 
Sbjct: 648 EVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKM 707

Query: 809 RKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           RK L H LL++EL+ QLKFP+KP DLKKRIESLIDR+Y+ERDK+NP  Y+Y+A
Sbjct: 708 RKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA 759

BLAST of MS013478 vs. ExPASy Swiss-Prot
Match: Q3TCH7 (Cullin-4A OS=Mus musculus OX=10090 GN=Cul4a PE=1 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.0e-234
Identity = 413/713 (57.92%), Postives = 527/713 (73.91%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           +  +P LP N+ +DTW KL  A+ AI        +LE+LYQAV +LC HK+   LY+++ 
Sbjct: 48  FRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLR 107

Query: 209 KECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSV 268
           + CE H+ A +      S D V+FLK +  CWQD C QM+MIR I L+LDRTYV Q   +
Sbjct: 108 QVCEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSML 167

Query: 269 CSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIY 328
            S+WDMGL+LFR H+     V+ KT+ G+L +I +ER GEAV+R+LL  LL M + L +Y
Sbjct: 168 PSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVY 227

Query: 329 SESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLI 388
            +SFE  FLE T+  YAAEG + MQ  +V EYL H  +RL+EE +R + YLD ST+KPLI
Sbjct: 228 KDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLI 287

Query: 389 ATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRT 448
           A  E+QLL  H++AIL KG   L+D NR+ DL +MY L SRV     +L Q  S YI+  
Sbjct: 288 ACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTF 347

Query: 449 GQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAE 508
           G  IV++ EKDKDMV  LL+FK  +D + E  F +NE F N +K++FE  IN R N+PAE
Sbjct: 348 GTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAE 407

Query: 509 LIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSA 568
           LIAK +D KLRAGNK  ++EELE  LDK+++LFRFI GKDVFEAFYKKDLAKRLL+GKSA
Sbjct: 408 LIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSA 467

Query: 569 SIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVH 628
           S+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I   FKQ  Q ++  P  I+++V+
Sbjct: 468 SVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQS-APGPIDLTVN 527

Query: 629 VLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGK 688
           +LT GYWPTY PM+V LP E+   Q++FK FYL K+SGR+L W  +LGH VLKA+F +GK
Sbjct: 528 ILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGK 587

Query: 689 KELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGR 748
           KE  VSLFQT+VL++FN+ +  SF++I+ +TGIED ELRRTLQSLACGK RVL K PKG+
Sbjct: 588 KEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGK 647

Query: 749 DVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKT 808
           +VED D F+FN  F   L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK 
Sbjct: 648 EVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKM 707

Query: 809 RKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           RK L H LL++EL+ QLKFP+KP DLKKRIESLIDR+Y+ERDK++P  Y+Y+A
Sbjct: 708 RKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA 759

BLAST of MS013478 vs. ExPASy TrEMBL
Match: A0A6J1CEB0 (cullin-4 OS=Momordica charantia OX=3673 GN=LOC111010756 PE=3 SV=1)

HSP 1 Score: 1571.2 bits (4067), Expect = 0.0e+00
Identity = 818/860 (95.12%), Postives = 818/860 (95.12%), Query Frame = 0

Query: 1   MSLPTKRSASTATAAASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60
           MSLPTKRSASTATA ASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD
Sbjct: 1   MSLPTKRSASTATATASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60

Query: 61  FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKKLVIKLLKGLFFRSHSAF 120
           FDPSSMALDEDLKPDDSPLIG SRAVATNLSRKKATPPQPAKKLVIKLLK          
Sbjct: 61  FDPSSMALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLK---------- 120

Query: 121 LFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDTWAKLKSAICAIFLKQPN 180
                                         AKPTLPANFEEDTWAKLKSAICAIFLKQPN
Sbjct: 121 ------------------------------AKPTLPANFEEDTWAKLKSAICAIFLKQPN 180

Query: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240
           SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW
Sbjct: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240

Query: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300
           QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM
Sbjct: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300

Query: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360
           IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY
Sbjct: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360

Query: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420
           LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL
Sbjct: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420

Query: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480
           QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF
Sbjct: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480

Query: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540
           SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF
Sbjct: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540

Query: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600
           RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL
Sbjct: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600

Query: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660
           SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL
Sbjct: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660

Query: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720
           SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI
Sbjct: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720

Query: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780
           EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE
Sbjct: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780

Query: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 840
           ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL
Sbjct: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 820

Query: 841 IDREYLERDKNNPQIYNYLA 861
           IDREYLERDKNNPQIYNYLA
Sbjct: 841 IDREYLERDKNNPQIYNYLA 820

BLAST of MS013478 vs. ExPASy TrEMBL
Match: A0A6J1EK69 (cullin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111434080 PE=3 SV=1)

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 788/877 (89.85%), Postives = 800/877 (91.22%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSS-ATSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSAS TA  AASSVVSS  TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSASATANTAASSVVSSLPTSNASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPCSNITSSAAADDADFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASID+EKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDSEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. ExPASy TrEMBL
Match: A0A6J1KQD7 (cullin-4-like OS=Cucurbita maxima OX=3661 GN=LOC111495542 PE=3 SV=1)

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 787/877 (89.74%), Postives = 800/877 (91.22%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSSA-TSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHHH 60
           MSLPTKRSAS TA  AASSVVSS+ TS ASI SPPMKKTK  SQAVP+SLDPNKNGLHHH
Sbjct: 1   MSLPTKRSASATANTAASSVVSSSPTSNASISSPPMKKTK--SQAVPTSLDPNKNGLHHH 60

Query: 61  --------------DPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D +FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPFSNITSSAAADDAEFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHIS ALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISVALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV 
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVS 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTA
Sbjct: 721 NDAEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 835

BLAST of MS013478 vs. ExPASy TrEMBL
Match: A0A6J1GSH7 (cullin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111457039 PE=3 SV=1)

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 783/877 (89.28%), Postives = 799/877 (91.11%), Query Frame = 0

Query: 1   MSLPTKRSAS-TATAAASSVVSSA-TSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHH- 60
           MSLPTKRSA+ TA  AASSVVSS+ TSTASI SPPMKKTK  SQ + +SLDPNKNGLHH 
Sbjct: 1   MSLPTKRSATPTANTAASSVVSSSPTSTASISSPPMKKTK--SQPLSTSLDPNKNGLHHL 60

Query: 61  -------------HDPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPSSNITSSALVDDADFDPSSMALDEDLKPDDSPLIGTSRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTP+VCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPNVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKG+KELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGRKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTA
Sbjct: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDK+NPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKSNPQIYNYLA 835

BLAST of MS013478 vs. ExPASy TrEMBL
Match: A0A6J1K265 (cullin-4-like OS=Cucurbita maxima OX=3661 GN=LOC111490376 PE=3 SV=1)

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 783/877 (89.28%), Postives = 797/877 (90.88%), Query Frame = 0

Query: 1   MSLPTKRSA-STATAAASSVVSSA-TSTASI-SPPMKKTKSHSQAVPSSLDPNKNGLHH- 60
           MSLPTKRSA +TA  AASSVVSS+ TSTASI SPPMKKTK  SQ + +SLDPNKNGLHH 
Sbjct: 1   MSLPTKRSATATANTAASSVVSSSPTSTASISSPPMKKTK--SQPLSTSLDPNKNGLHHL 60

Query: 61  -------------HDPDFDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKK 120
                         D DFDPSSMALDEDL PDDSPLIG SRAVATNLSRKKAT PQPAKK
Sbjct: 61  DRPSSNITSSALVDDADFDPSSMALDEDLNPDDSPLIGTSRAVATNLSRKKATLPQPAKK 120

Query: 121 LVIKLLKGLFFRSHSAFLFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDT 180
           LVIKL+K                                        AKPTLPANFEEDT
Sbjct: 121 LVIKLVK----------------------------------------AKPTLPANFEEDT 180

Query: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 240
           WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLV
Sbjct: 181 WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLV 240

Query: 241 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300
           GQSPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL
Sbjct: 241 GQSPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 300

Query: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360
           SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF
Sbjct: 301 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 360

Query: 361 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 420
           YAAEGMK+MQ S VSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAI
Sbjct: 361 YAAEGMKHMQQSYVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAI 420

Query: 421 LDKGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480
           LDKGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP
Sbjct: 421 LDKGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVP 480

Query: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540
           SLLEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKG
Sbjct: 481 SLLEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKG 540

Query: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600
           TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC
Sbjct: 541 TSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTEC 600

Query: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660
           GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR
Sbjct: 601 GSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVR 660

Query: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720
           LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF
Sbjct: 661 LPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLF 720

Query: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTA 780
           NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTA
Sbjct: 721 NDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTA 780

Query: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 840
           PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 781 PLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 835

Query: 841 LKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           LKFPIKPADLKKRIESLIDREYLERDK+NPQIYNYLA
Sbjct: 841 LKFPIKPADLKKRIESLIDREYLERDKSNPQIYNYLA 835

BLAST of MS013478 vs. TAIR 10
Match: AT5G46210.1 (cullin4 )

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 659/860 (76.63%), Postives = 703/860 (81.74%), Query Frame = 0

Query: 1   MSLPTKRSASTATAAASSVVSSATSTASISPPMKKTKSHSQAVPSSLDPNKNGLHHHDPD 60
           MSLPTKR  ST +AA++S  SS +     SPPMKK               KN LHH    
Sbjct: 1   MSLPTKR--STFSAASASDDSSYS-----SPPMKKA--------------KNDLHHSPQH 60

Query: 61  FDPSSMALDEDLKPDDSPLIGASRAVATNLSRKKATPPQPAKKLVIKLLKGLFFRSHSAF 120
            + +   +   ++ D +P        A NLSRKKAT PQP KK VIKL K          
Sbjct: 61  PNTADKVVGFHMEEDPTP-------AAANLSRKKATLPQPTKKFVIKLNK---------- 120

Query: 121 LFLGHESYPVKLISCVENGLEFLNFHFLYEAKPTLPANFEEDTWAKLKSAICAIFLKQPN 180
                                         AKPTLP NFEE+TW KL+SAI AIFLK+  
Sbjct: 121 ------------------------------AKPTLPTNFEENTWEKLQSAIRAIFLKKKI 180

Query: 181 SCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCW 240
           S DLE LYQAV++LCLHK+ G LY +IEKECE HISAALQSLVGQ+ DL VFL  VEKCW
Sbjct: 181 SFDLESLYQAVDNLCLHKLDGKLYDQIEKECEEHISAALQSLVGQNTDLTVFLSRVEKCW 240

Query: 241 QDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRM 300
           QDFCDQMLMIR IAL LDR YV Q P+V SLW+MGLQLFRKHLSL+ EVE +TV GLL M
Sbjct: 241 QDFCDQMLMIRSIALTLDRKYVIQNPNVRSLWEMGLQLFRKHLSLAPEVEQRTVKGLLSM 300

Query: 301 IEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEY 360
           IEKERL EAVNRTLL+HLLKMFTALGIY ESFEKPFLE TSEFYAAEGMKYMQ SDV EY
Sbjct: 301 IEKERLAEAVNRTLLSHLLKMFTALGIYMESFEKPFLEGTSEFYAAEGMKYMQQSDVPEY 360

Query: 361 LKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDL 420
           LKH E RL EENERC+LY+D  TRKPLI T ERQLLERHI  +L+KGFT LMDG R EDL
Sbjct: 361 LKHVEGRLHEENERCILYIDAVTRKPLITTVERQLLERHILVVLEKGFTTLMDGRRTEDL 420

Query: 421 QRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESF 480
           QRM TL SRVNALESLRQALSSY+R+TGQ IVMD+EKDKDMV SLL+FKASLD IWEESF
Sbjct: 421 QRMQTLFSRVNALESLRQALSSYVRKTGQKIVMDEEKDKDMVQSLLDFKASLDIIWEESF 480

Query: 481 SKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLF 540
            KNE+F NTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELE  L+KVLVLF
Sbjct: 481 YKNESFGNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELESVLEKVLVLF 540

Query: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600
           RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL
Sbjct: 541 RFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIEL 600

Query: 601 SKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYL 660
           SKEINESFKQSSQARTKLP GIEMSVHVLTTGYWPTYPPMDV+LPHELNVYQDIFKEFYL
Sbjct: 601 SKEINESFKQSSQARTKLPSGIEMSVHVLTTGYWPTYPPMDVKLPHELNVYQDIFKEFYL 660

Query: 661 SKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGI 720
           SKYSGRRLMW NSLGHCVLKA+F KGKKELAVSLFQ VVLMLFNDA KLSF+DI++ST I
Sbjct: 661 SKYSGRRLMWQNSLGHCVLKADFSKGKKELAVSLFQAVVLMLFNDAMKLSFEDIKDSTSI 720

Query: 721 EDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVE 780
           EDKELRRTLQSLACGKVRVLQK PKGRDVED D F FN+ F APLYR+KVNAIQMKETVE
Sbjct: 721 EDKELRRTLQSLACGKVRVLQKNPKGRDVEDGDEFEFNDEFAAPLYRIKVNAIQMKETVE 780

Query: 781 ENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 840
           ENTSTTERVFQDRQYQ+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL
Sbjct: 781 ENTSTTERVFQDRQYQIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESL 792

Query: 841 IDREYLERDKNNPQIYNYLA 861
           IDREYLER+K+NPQIYNYLA
Sbjct: 841 IDREYLEREKSNPQIYNYLA 792

BLAST of MS013478 vs. TAIR 10
Match: AT1G69670.1 (cullin 3B )

HSP 1 Score: 519.6 bits (1337), Expect = 4.7e-147
Identity = 288/726 (39.67%), Postives = 452/726 (62.26%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           ++ +  +   + + TW  L+ AI  I+    +    E+LY+   ++ LHK G  LY  + 
Sbjct: 14  FKQRVVVDPKYADKTWKILEHAIHEIYNHNASGLSFEELYRNAYNMVLHKYGDKLYTGLV 73

Query: 209 KECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSV 268
                H+    +S+  +      FL+ + + W D    + MIR I +Y+DRTYV  T   
Sbjct: 74  TTMTFHLKEICKSI--EEAQGGAFLELLNRKWNDHNKALQMIRDILMYMDRTYVSTTKKT 133

Query: 269 CSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALG-- 328
             + ++GL L+R ++  SS+++ + +  LL ++ KER GE ++R L+ +++KMF  LG  
Sbjct: 134 -HVHELGLHLWRDNVVYSSKIQTRLLNTLLDLVHKERTGEVIDRVLMRNVIKMFMDLGES 193

Query: 329 IYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKP 388
           +Y + FEKPFLE ++EFY  E M++++  D  EYLK AE+ L EE ER + YLD  +   
Sbjct: 194 VYQDDFEKPFLEASAEFYKVESMEFIESCDCGEYLKKAEKPLVEEVERVVNYLDAKSEAK 253

Query: 389 LIATAERQLLERHISAIL---DKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSS 448
           + +  ER+++  H+  ++   + G   ++  ++ ED+ RMY+L  RV N L ++R  ++ 
Sbjct: 254 ITSVVEREMIANHVQRLVHMENSGLVNMLLNDKYEDMGRMYSLFRRVANGLVTVRDVMTL 313

Query: 449 YIRRTGQNIVMDDEKDKD---MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLIN 508
           ++R  G+ +V D EK KD    V  LL+ +   D I   +F+ ++ F N +  +FE+ +N
Sbjct: 314 HLREMGKQLVTDPEKSKDPVEFVQRLLDERDKYDRIINMAFNNDKTFQNALNSSFEYFVN 373

Query: 509 LRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAK 568
           L    P E I+ F+D+KLR G KG  EE+++  LDKV++LFR++Q KDVFE +YK+ LAK
Sbjct: 374 LNTRSP-EFISLFVDDKLRKGLKGVGEEDVDLILDKVMMLFRYLQEKDVFEKYYKQHLAK 433

Query: 569 RLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLP 628
           RLL GK+ S DAE+++I KLKTECG QFT+KLEGMF D++ S +    F  S     +L 
Sbjct: 434 RLLSGKTVSDDAERNLIVKLKTECGYQFTSKLEGMFTDMKTSHDTLLGFYNS---HPELS 493

Query: 629 MGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVL 688
            G  + V VLTTG WPT P +   LP E++V  + F+ +YL  ++GRRL W  ++G   +
Sbjct: 494 EGPTLVVQVLTTGSWPTQPTIQCNLPAEVSVLCEKFRSYYLGTHTGRRLSWQTNMGTADI 553

Query: 689 KAEFPKGKK-ELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVR 748
           KA F KG+K EL VS FQ  VLMLFN++++LS+++I ++T I   +L+R LQS+AC K +
Sbjct: 554 KAVFGKGQKHELNVSTFQMCVLMLFNNSDRLSYKEIEQATEIPTPDLKRCLQSMACVKGK 613

Query: 749 -VLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKV-NAIQMKETVEENTSTTERVFQDRQYQ 808
            VL+K P  +++ + D FV N+ F +  Y++K+   +  KET  E   T +RV +DR+ Q
Sbjct: 614 NVLRKEPMSKEIAEEDWFVVNDRFASKFYKVKIGTVVAQKETEPEKQETRQRVEEDRKPQ 673

Query: 809 VDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKNNPQ 861
           ++AAIVRIMK+R+VL H  +I E+ +QL  +F   P ++KKRIESLI+R++LERD  + +
Sbjct: 674 IEAAIVRIMKSRRVLDHNNIIAEVTKQLQTRFLANPTEIKKRIESLIERDFLERDNTDRK 732

BLAST of MS013478 vs. TAIR 10
Match: AT1G26830.1 (cullin 3 )

HSP 1 Score: 516.9 bits (1330), Expect = 3.1e-146
Identity = 291/729 (39.92%), Postives = 455/729 (62.41%), Query Frame = 0

Query: 149 YEAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIE 208
           ++ +  +   + + TW  L+ AI  I+ +  +    E+LY+   ++ LHK G  LY    
Sbjct: 14  FKHRVVVDPKYADKTWQILERAIHQIYNQDASGLSFEELYRNAYNMVLHKFGEKLYTGFI 73

Query: 209 KECEVHI---SAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQT 268
                H+   S  +++  G S     FL+ + K W +    + MIR I +Y+DRTY++ T
Sbjct: 74  ATMTSHLKEKSKLIEAAQGGS-----FLEELNKKWNEHNKALEMIRDILMYMDRTYIEST 133

Query: 269 PSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTAL 328
                +  MGL L+R ++   +++  + +  LL +++KER+GE ++R L+ +++KMF  L
Sbjct: 134 KKT-HVHPMGLNLWRDNVVHFTKIHTRLLNTLLDLVQKERIGEVIDRGLMRNVIKMFMDL 193

Query: 329 G--IYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTST 388
           G  +Y E FEKPFL+ +SEFY  E  ++++  D  +YLK +E+RL EE ER   YLD  +
Sbjct: 194 GESVYQEDFEKPFLDASSEFYKVESQEFIESCDCGDYLKKSEKRLTEEIERVAHYLDAKS 253

Query: 389 RKPLIATAERQLLERHISAIL---DKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQA 448
            + + +  E++++  H+  ++   + G   ++  ++ EDL RMY L  RV N L ++R  
Sbjct: 254 EEKITSVVEKEMIANHMQRLVHMENSGLVNMLLNDKYEDLGRMYNLFRRVTNGLVTVRDV 313

Query: 449 LSSYIRRTGQNIVMDDEKDKD---MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEH 508
           ++S++R  G+ +V D EK KD    V  LL+ +   D I   +F  ++ F N +  +FE+
Sbjct: 314 MTSHLREMGKQLVTDPEKSKDPVEFVQRLLDERDKYDKIINTAFGNDKTFQNALNSSFEY 373

Query: 509 LINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKD 568
            INL    P E I+ F+D+KLR G KG ++ ++E  LDKV++LFR++Q KDVFE +YK+ 
Sbjct: 374 FINLNARSP-EFISLFVDDKLRKGLKGITDVDVEVILDKVMMLFRYLQEKDVFEKYYKQH 433

Query: 569 LAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQART 628
           LAKRLL GK+ S DAE+S+I KLKTECG QFT+KLEGMF D++ S++    F  S     
Sbjct: 434 LAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSEDTMRGFYGS---HP 493

Query: 629 KLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGH 688
           +L  G  + V VLTTG WPT P +   LP E++V  + F+ +YL  ++GRRL W  ++G 
Sbjct: 494 ELSEGPTLIVQVLTTGSWPTQPAVPCNLPAEVSVLCEKFRSYYLGTHTGRRLSWQTNMGT 553

Query: 689 CVLKAEFPKGKK-ELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACG 748
             +KA F KG+K EL VS FQ  VLMLFN++++LS+++I ++T I   +L+R LQSLAC 
Sbjct: 554 ADIKAIFGKGQKHELNVSTFQMCVLMLFNNSDRLSYKEIEQATEIPAADLKRCLQSLACV 613

Query: 749 KVR-VLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKV-NAIQMKETVEENTSTTERVFQDR 808
           K + V++K P  +D+ + D FV N+ FT+  Y++K+   +  KET  E   T +RV +DR
Sbjct: 614 KGKNVIKKEPMSKDIGEEDLFVVNDKFTSKFYKVKIGTVVAQKETEPEKQETRQRVEEDR 673

Query: 809 QYQVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKN 861
           + Q++AAIVRIMK+RK+L H  +I E+ +QL  +F   P ++KKRIESLI+R++LERD  
Sbjct: 674 KPQIEAAIVRIMKSRKILDHNNIIAEVTKQLQPRFLANPTEIKKRIESLIERDFLERDST 732

BLAST of MS013478 vs. TAIR 10
Match: AT4G02570.1 (cullin 1 )

HSP 1 Score: 384.4 bits (986), Expect = 2.4e-106
Identity = 238/705 (33.76%), Postives = 386/705 (54.75%), Query Frame = 0

Query: 187 LYQAVNDLCL----HKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQD 246
           LY  + ++C     H     LY +  +  E +I++ +   + +  D    L+ + K W +
Sbjct: 42  LYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHD-EFMLRELFKRWSN 101

Query: 247 FCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIE 306
               +  +     YLDR ++ +  S+  L ++GL  FR    + +E+  K    ++ +++
Sbjct: 102 HKVMVRWLSRFFYYLDRYFIARR-SLPPLNEVGLTCFRD--LVYNELHSKVKQAVIALVD 161

Query: 307 KERLGEAVNRTLLNHLLKMFTALGI-----YSESFEKPFLEYTSEFYAAEGMKYMQLSDV 366
           KER GE ++R LL ++L ++  +G+     Y E FE   L+ TS +Y+ +   ++Q    
Sbjct: 162 KEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSC 221

Query: 367 SEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDK---GFTLLMDG 426
            +Y+  +EE L++E ER   YL +S+   L+   + +LL    S +L+K   G   L+  
Sbjct: 222 PDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRD 281

Query: 427 NRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDE-------------KDKD 486
           ++++DL RMY L  ++   LE +      ++   G  +V   E             +++ 
Sbjct: 282 DKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQV 341

Query: 487 MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLIN--LRQNRPAELIAKFLDEKL- 546
           ++  ++E          E F  +  F   +K+AFE   N  +  +  AEL+A F D  L 
Sbjct: 342 LIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILK 401

Query: 547 RAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMIS 606
           + G++  S+E +E TL+KV+ L  +I  KD+F  FY+K LA+RLL  +SA+ D E+S+++
Sbjct: 402 KGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILT 461

Query: 607 KLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTY 666
           KLK +CG QFT+K+EGM  D+ L++E   SF+    +      GI+++V VLTTG+WP+Y
Sbjct: 462 KLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSY 521

Query: 667 PPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQT 726
              D+ LP E+    ++FK FY +K   R+L W  SLG C +  +F +   EL VS +Q 
Sbjct: 522 KSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQA 581

Query: 727 VVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVF 786
            VL+LFN  +KLS+ +I     +  ++L R L SL+C K ++L K P  + V  ND+F F
Sbjct: 582 AVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEF 641

Query: 787 NEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLI 846
           N  FT  + R+K+        V+E     E V +DR+Y +DAAIVRIMK+RKVL H  L+
Sbjct: 642 NSKFTDRMRRIKIPL----PPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLV 701

Query: 847 TELFQQLKFPIKP--ADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           +E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct: 702 SECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

BLAST of MS013478 vs. TAIR 10
Match: AT4G02570.2 (cullin 1 )

HSP 1 Score: 384.4 bits (986), Expect = 2.4e-106
Identity = 238/705 (33.76%), Postives = 386/705 (54.75%), Query Frame = 0

Query: 187 LYQAVNDLCL----HKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQD 246
           LY  + ++C     H     LY +  +  E +I++ +   + +  D    L+ + K W +
Sbjct: 42  LYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHD-EFMLRELFKRWSN 101

Query: 247 FCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIE 306
               +  +     YLDR ++ +  S+  L ++GL  FR    + +E+  K    ++ +++
Sbjct: 102 HKVMVRWLSRFFYYLDRYFIARR-SLPPLNEVGLTCFRD--LVYNELHSKVKQAVIALVD 161

Query: 307 KERLGEAVNRTLLNHLLKMFTALGI-----YSESFEKPFLEYTSEFYAAEGMKYMQLSDV 366
           KER GE ++R LL ++L ++  +G+     Y E FE   L+ TS +Y+ +   ++Q    
Sbjct: 162 KEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSC 221

Query: 367 SEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDK---GFTLLMDG 426
            +Y+  +EE L++E ER   YL +S+   L+   + +LL    S +L+K   G   L+  
Sbjct: 222 PDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRD 281

Query: 427 NRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDE-------------KDKD 486
           ++++DL RMY L  ++   LE +      ++   G  +V   E             +++ 
Sbjct: 282 DKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQV 341

Query: 487 MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLIN--LRQNRPAELIAKFLDEKL- 546
           ++  ++E          E F  +  F   +K+AFE   N  +  +  AEL+A F D  L 
Sbjct: 342 LIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILK 401

Query: 547 RAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMIS 606
           + G++  S+E +E TL+KV+ L  +I  KD+F  FY+K LA+RLL  +SA+ D E+S+++
Sbjct: 402 KGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILT 461

Query: 607 KLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTY 666
           KLK +CG QFT+K+EGM  D+ L++E   SF+    +      GI+++V VLTTG+WP+Y
Sbjct: 462 KLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSY 521

Query: 667 PPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQT 726
              D+ LP E+    ++FK FY +K   R+L W  SLG C +  +F +   EL VS +Q 
Sbjct: 522 KSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQA 581

Query: 727 VVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVF 786
            VL+LFN  +KLS+ +I     +  ++L R L SL+C K ++L K P  + V  ND+F F
Sbjct: 582 AVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEF 641

Query: 787 NEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLI 846
           N  FT  + R+K+        V+E     E V +DR+Y +DAAIVRIMK+RKVL H  L+
Sbjct: 642 NSKFTDRMRRIKIPL----PPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLV 701

Query: 847 TELFQQLKFPIKP--ADLKKRIESLIDREYLERDKNNPQIYNYLA 861
           +E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct: 702 SECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738