MS013477 (gene) Bitter gourd (TR) v1

BLAST of MS013477 vs. NCBI nr
Match: XP_038877172.1 (uncharacterized protein LOC120069472 [Benincasa hispida])

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-30
Identity = 106/187 (56.68%), Postives = 117/187 (62.57%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIF--TFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQ 73
           A FSSL  L+ + IIF   FKNLS  S LFNST FWFFISN L I IIA DY  +FS  Q
Sbjct: 40  AFFSSLLFLLSIFIIFISIFKNLSLPS-LFNSTIFWFFISNTL-IFIIAVDYG-VFSLPQ 99

Query: 74  NKRLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRRDFGS-------- 133
           +K    YED+  SN         SS  V+ DE++ET  EKL++VV  R   S        
Sbjct: 100 HKSFHLYEDFSPSNPKLNHFRLPSSSLVVFDEKKETTREKLELVVQGRQLDSPWKMTPAR 159

Query: 134 -CRRSKSEKPK-------RKTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRI 182
            CRR KSEKPK        K M +RSESVKYEAK  E NEF KMTDEELNRRVEEFIQR 
Sbjct: 160 TCRRRKSEKPKGIVSKESNKMMGKRSESVKYEAKELEENEFEKMTDEELNRRVEEFIQRF 219

BLAST of MS013477 vs. NCBI nr
Match: XP_022927149.1 (uncharacterized protein LOC111434084 [Cucurbita moschata])

HSP 1 Score: 135.6 bits (340), Expect = 4.3e-28
Identity = 105/186 (56.45%), Postives = 115/186 (61.83%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQNK 73
           A FSSL  L+LLS    FKNLSFSS LFNST FWFFISNAL I IIAADY   FS +Q K
Sbjct: 36  AFFSSL--LLLLSFCIIFKNLSFSS-LFNSTMFWFFISNAL-IFIIAADY-AYFSLSQYK 95

Query: 74  RLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRR----------DFGS 133
           R   YE Y   N   T     +S  V+ DE+RE P+  LQ  V  R             +
Sbjct: 96  RSHLYEHYSPPNPKMTHFQHQTSSFVVFDEKREYPDGNLQNFVPERCDSLPPLKTTPVRT 155

Query: 134 CRRSKSEKPKR-------KTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRIN 182
            RRSKSEKPKR       K M +RSES KYE K  E NE+SKMTDEELNRRVEEFIQR N
Sbjct: 156 YRRSKSEKPKRSVSKESKKKMAKRSESGKYEEKELEENEYSKMTDEELNRRVEEFIQRFN 215

BLAST of MS013477 vs. NCBI nr
Match: XP_023001467.1 (uncharacterized protein LOC111495593 [Cucurbita maxima])

HSP 1 Score: 132.1 bits (331), Expect = 4.8e-27
Identity = 103/186 (55.38%), Postives = 115/186 (61.83%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQNK 73
           A FSSL  L LLS    FKNLSFSS LFNST FWFFISN L I IIAADY   FS +Q K
Sbjct: 36  AFFSSL--LFLLSFCIIFKNLSFSS-LFNSTIFWFFISNTL-IFIIAADY-AYFSLSQYK 95

Query: 74  RLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRR----------DFGS 133
           R  FYE Y   N   T     +S  V+ +E+RE P+  LQ +V  R             +
Sbjct: 96  RSHFYEHYSPPNPKITHFQHQTSSFVVFEEKREYPDGNLQNIVQDRCDSLPPLKTTPVRT 155

Query: 134 CRRSKSEKPKR-------KTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRIN 182
            +RSKSEKPKR       K M +RSES KYE K  E NE+SKMTDEELNRRVEEFIQR N
Sbjct: 156 YQRSKSEKPKRTVSKESKKKMAKRSESGKYEGKELEENEYSKMTDEELNRRVEEFIQRFN 215

BLAST of MS013477 vs. NCBI nr
Match: KAE8653186.1 (hypothetical protein Csa_020019 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 6.3e-27
Identity = 109/195 (55.90%), Postives = 124/195 (63.59%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIF--TFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQ 73
           A FSSL  L+ + IIF   FKNLSFSS LFNST FWFFISN L I IIA DY  +FS +Q
Sbjct: 42  AFFSSLLFLLSIFIIFISIFKNLSFSS-LFNSTVFWFFISNTL-IFIIAVDYG-LFSLSQ 101

Query: 74  NKRLDFYEDYVSSNQNQTQI---LQSSSLAVIVDEERETPEEKLQIVVGRRDFGS----- 133
           +K    YEDY  S  N       LQ+SSL V+ DE+RETP+EKL+ VV  +   S     
Sbjct: 102 HKSFHLYEDYYYSPPNPKLTHFQLQTSSL-VVFDEKRETPDEKLETVVQSQRLDSPSKNT 161

Query: 134 ------CRRSKSEKPKR--------KTM-ERRSESVKYEAK--GEGNEFSKMTDEELNRR 182
                   R KSEKPKR        K M +RRSESVK E K   + NEF+KMTDEELNRR
Sbjct: 162 TTPVRTYLRRKSEKPKRIVSMEISKKMMGKRRSESVKNEGKELEDENEFAKMTDEELNRR 221

BLAST of MS013477 vs. NCBI nr
Match: XP_023520225.1 (uncharacterized protein LOC111783530 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 101/186 (54.30%), Postives = 112/186 (60.22%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQNK 73
           A FSSL  L+   II  FKNLSFSS LFNST FWFFISNAL I IIAADY   FS TQ K
Sbjct: 3   AFFSSLLFLLFFCII--FKNLSFSS-LFNSTMFWFFISNAL-IFIIAADY-AYFSLTQYK 62

Query: 74  RLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRR----------DFGS 133
           R   YE Y   N   T   + +S  V+ DE+RE P+  L+  V  R             +
Sbjct: 63  RSHLYEHYSPPNPKMTHFQRQTSSFVVFDEKREYPDGNLRNFVQERCDSMPLLKTTPVRT 122

Query: 134 CRRSKSEKPKR-------KTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRIN 182
             RSKSEKPKR       K M +R ES  YE K  E NE+SKMTDEELNRRVEEFIQR N
Sbjct: 123 YERSKSEKPKRSVSKESKKKMAKRLESGMYEEKELEENEYSKMTDEELNRRVEEFIQRFN 182

BLAST of MS013477 vs. ExPASy TrEMBL
Match: A0A6J1EH73 (uncharacterized protein LOC111434084 OS=Cucurbita moschata OX=3662 GN=LOC111434084 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.1e-28
Identity = 105/186 (56.45%), Postives = 115/186 (61.83%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQNK 73
           A FSSL  L+LLS    FKNLSFSS LFNST FWFFISNAL I IIAADY   FS +Q K
Sbjct: 36  AFFSSL--LLLLSFCIIFKNLSFSS-LFNSTMFWFFISNAL-IFIIAADY-AYFSLSQYK 95

Query: 74  RLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRR----------DFGS 133
           R   YE Y   N   T     +S  V+ DE+RE P+  LQ  V  R             +
Sbjct: 96  RSHLYEHYSPPNPKMTHFQHQTSSFVVFDEKREYPDGNLQNFVPERCDSLPPLKTTPVRT 155

Query: 134 CRRSKSEKPKR-------KTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRIN 182
            RRSKSEKPKR       K M +RSES KYE K  E NE+SKMTDEELNRRVEEFIQR N
Sbjct: 156 YRRSKSEKPKRSVSKESKKKMAKRSESGKYEEKELEENEYSKMTDEELNRRVEEFIQRFN 215

BLAST of MS013477 vs. ExPASy TrEMBL
Match: A0A6J1KGM0 (uncharacterized protein LOC111495593 OS=Cucurbita maxima OX=3661 GN=LOC111495593 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.3e-27
Identity = 103/186 (55.38%), Postives = 115/186 (61.83%), Query Frame = 0

Query: 14  ALFSSLASLILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILIIAADYNRIFSPTQNK 73
           A FSSL  L LLS    FKNLSFSS LFNST FWFFISN L I IIAADY   FS +Q K
Sbjct: 36  AFFSSL--LFLLSFCIIFKNLSFSS-LFNSTIFWFFISNTL-IFIIAADY-AYFSLSQYK 95

Query: 74  RLDFYEDYVSSNQNQTQILQSSSLAVIVDEERETPEEKLQIVVGRR----------DFGS 133
           R  FYE Y   N   T     +S  V+ +E+RE P+  LQ +V  R             +
Sbjct: 96  RSHFYEHYSPPNPKITHFQHQTSSFVVFEEKREYPDGNLQNIVQDRCDSLPPLKTTPVRT 155

Query: 134 CRRSKSEKPKR-------KTMERRSESVKYEAKG-EGNEFSKMTDEELNRRVEEFIQRIN 182
            +RSKSEKPKR       K M +RSES KYE K  E NE+SKMTDEELNRRVEEFIQR N
Sbjct: 156 YQRSKSEKPKRTVSKESKKKMAKRSESGKYEGKELEENEYSKMTDEELNRRVEEFIQRFN 215

BLAST of MS013477 vs. ExPASy TrEMBL
Match: A0A0A0M033 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435810 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.2e-20
Identity = 88/163 (53.99%), Postives = 101/163 (61.96%), Query Frame = 0

Query: 44  TNFWFFISNALIILIIAADYNRIFSPTQNKRLDFYEDYVSSNQNQTQI---LQSSSLAVI 103
           T FWFFISN L I IIA DY  +FS +Q+K    YEDY  S  N       LQ+SSL V+
Sbjct: 41  TVFWFFISNTL-IFIIAVDYG-LFSLSQHKSFHLYEDYYYSPPNPKLTHFQLQTSSL-VV 100

Query: 104 VDEERETPEEKLQIVVGRRDFGS-----------CRRSKSEKPKR--------KTM-ERR 163
            DE+RETP+EKL+ VV  +   S             R KSEKPKR        K M +RR
Sbjct: 101 FDEKRETPDEKLETVVQSQRLDSPSKNTTTPVRTYLRRKSEKPKRIVSMEISKKMMGKRR 160

Query: 164 SESVKYEAK--GEGNEFSKMTDEELNRRVEEFIQRINRQMRLQ 182
           SESVK E K   + NEF+KMTDEELNRRVEEFIQR N+QMRLQ
Sbjct: 161 SESVKNEGKELEDENEFAKMTDEELNRRVEEFIQRFNKQMRLQ 200

BLAST of MS013477 vs. ExPASy TrEMBL
Match: A0A7J7CW61 (Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW13G01217 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.8e-16
Identity = 86/205 (41.95%), Postives = 116/205 (56.59%), Query Frame = 0

Query: 1   KSNNPPYKFAKGAALFSSLASLIL-LSIIFTFKNLSFSSHLFNSTNFWFFISNALIILII 60
           K +  P K  KG + F  + S+++ +SI  TFK LS  S+LFN+T FWFFISN L ILII
Sbjct: 9   KQDYYPIKTHKGMSFFGFIFSIVVYISIFHTFK-LS-PSNLFNNTKFWFFISNTL-ILII 68

Query: 61  AADYNRIFSPTQNKRLDFYEDYVSSNQNQTQILQSSSLAV-IVDEERE--TPEEKLQIVV 120
             DY   FS ++N   D YE++V       +   + SL V  V+E +E  T E   Q+V 
Sbjct: 69  YLDYG-AFSSSKNNNQDLYEEFVRRTSQPRRSTVAPSLDVEDVEEIKEVITSENNAQLVQ 128

Query: 121 GRR-----DFGSCRRSKSEKPKR---------------KTMERRSESVKYEAKGEGNEFS 180
            ++     +    RRSKSE+ KR               +  E+  E   ++ +GE NEFS
Sbjct: 129 EKKRENEIEDKKHRRSKSERIKRVNFDESKNVIIRSSSEIAEKLDELPSWQKEGEENEFS 188

Query: 181 KMTDEELNRRVEEFIQRINRQMRLQ 182
            M++EELNRRVEEFIQR NRQ+RLQ
Sbjct: 189 NMSNEELNRRVEEFIQRFNRQIRLQ 209

BLAST of MS013477 vs. ExPASy TrEMBL
Match: A0A2N9GT34 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30393 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.1e-16
Identity = 86/195 (44.10%), Postives = 117/195 (60.00%), Query Frame = 0

Query: 1   KSNNPPYKFAKGAALFSSLASL-ILLSIIFTFKNLSFSSHLFNSTNFWFFISNALIILII 60
           KS+    + A G + ++ L S+ I +SI++ F NLS  S LFN+T FWFF+SN L ILII
Sbjct: 18  KSSKEYPQKANGMSFYAFLFSIFIYISILYIF-NLS-PSTLFNTTKFWFFLSNTL-ILII 77

Query: 61  AADYNRIFSPTQNKRLDFYEDYVSSNQ--NQTQILQS--SSLAVIVDEERETPEEKLQIV 120
           A DY   +S ++ K+ DFY++YV   Q  N    +     ++  +V+  +E  +EK+Q  
Sbjct: 78  AVDYG-AYSSSKEKQ-DFYQEYVMDTQVKNVPSFVSQHPETIKEVVEVLQEPEKEKIQA- 137

Query: 121 VGRRDFGSCRRSKSEKPKRKTME-------RRSESVKYEAKG--EGNEFSKMTDEELNRR 180
                    RRSKSEK KR  ++       R SE+ K+E     E NEFS M+DEELNRR
Sbjct: 138 ------KPYRRSKSEKTKRVLIDESKNIVLRMSETAKHERTPSLEENEFSSMSDEELNRR 197

Query: 181 VEEFIQRINRQMRLQ 182
           VEEFIQR NRQ+RLQ
Sbjct: 198 VEEFIQRFNRQIRLQ 200

BLAST of MS013477 vs. TAIR 10
Match: AT1G30190.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G34610.1); Has 56 Blast hits to 56 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-05
Identity = 78/266 (29.32%), Postives = 113/266 (42.48%), Query Frame = 0

Query: 4   NPPYKFAKGAALFSSLASLIL----LSIIFTFKNLSFSSHLFNSTNFWFFISNALIILII 63
           NP ++  K     SS+ ++ L      +IF    +S SS +F  T   FFISN L ILII
Sbjct: 11  NPKFEPKKRRYYSSSMLTIFLPIFTYIMIFHVFEVSLSS-VFKDTKVLFFISNTL-ILII 70

Query: 64  AADYNRIFSPTQNKRLDFYEDY-VSSNQNQTQILQSSSLAVIV----------------- 123
           AADY   FS  +++  DFY +Y V++   + +    S + V+                  
Sbjct: 71  AADYGS-FSDKESQ--DFYGEYTVAAATMRNRADNYSPIPVLTYRENTKDGEIKNPKDVE 130

Query: 124 ----DEERE---------TPEEKLQIVVGRR--------------------DFGSCR--- 182
               +EE E         +P EK+  VV  +                       +C    
Sbjct: 131 FRNPEEEDEPMVKDIICVSPPEKIVRVVSEKKQRDDVAMEEYKPVTEQTLASEEACNTRN 190

BLAST of MS013477 vs. TAIR 10
Match: AT2G34610.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G30190.1); Has 342 Blast hits to 279 proteins in 74 species: Archae - 0; Bacteria - 7; Metazoa - 76; Fungi - 18; Plants - 51; Viruses - 0; Other Eukaryotes - 190 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-05
Identity = 80/279 (28.67%), Postives = 105/279 (37.63%), Query Frame = 0

Query: 6   PYKFAKGAALFSSLASLIL--LSIIFTFKNLSFSS-HLFNSTNFWFFISNALIILIIAAD 65
           PYK  K   L+SSL  +IL   + I  F  L  S   +FN T   F ISNALII IIAAD
Sbjct: 16  PYKPKKQYKLYSSLLPIILSIFTYILIFHVLDVSPLSIFNDTKILFVISNALII-IIAAD 75

Query: 66  YNRIFSPTQNKRLDFYEDYVSSNQNQTQ---ILQSSSLAVIVDEERETPEEKLQIVVGRR 125
           Y      T  + LDFY +Y ++ +   Q     + S   V + EE +   EK +  VG R
Sbjct: 76  YGAF---TDKENLDFYGEYTAAMRRDAQENPRPEKSVYGVSMAEETKN-REKQEGPVGAR 135

Query: 126 DF------------------------------------------------GSCR------ 182
           D                                                  +C       
Sbjct: 136 DLQDQYLPNKKAKVTERIIQAVSKNQPRNKAIEKCEPVTEQNVHIIAAKEETCNASNLMN 195