MS013473 (gene) Bitter gourd (TR) v1

BLAST of MS013473 vs. NCBI nr
Match: XP_038894014.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 981.1 bits (2535), Expect = 4.2e-282
Identity = 457/578 (79.07%), Postives = 514/578 (88.93%), Query Frame = 0

Query: 15  MGLGMGFSARPINI-GKKPNQKNEGISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPP 74
           MGLG+G   RPINI  KKPNQ+NE IS  L SPSVFHFP+QPNK+PRITE+ + +CRHP 
Sbjct: 1   MGLGLGSPTRPINIVKKKPNQRNEVISSPLNSPSVFHFPKQPNKQPRITEDVTFKCRHPS 60

Query: 75  HWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGT 134
           HWN LQKGASMA D+LEQA L NNDNRNLPK FDP VQIAGNYAPVPE PV H LPVTG 
Sbjct: 61  HWNLLQKGASMAFDILEQAFL-NNDNRNLPKTFDPHVQIAGNYAPVPEHPVSHSLPVTGV 120

Query: 135 IPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQERA 194
           IPDCINGVYLRNGANP FEPLAGHHLFDGDGM+HAVTIGGGRASYACRFTETERL+QE+A
Sbjct: 121 IPDCINGVYLRNGANPFFEPLAGHHLFDGDGMIHAVTIGGGRASYACRFTETERLVQEKA 180

Query: 195 IGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSED 254
           IGRPVFPKA+GELHGHSGIARLLLF+ RGLFGLIDH  GTGVANAGLVYFNGRLLAMSED
Sbjct: 181 IGRPVFPKAVGELHGHSGIARLLLFYARGLFGLIDHHHGTGVANAGLVYFNGRLLAMSED 240

Query: 255 DLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFW 314
           DLPYHVRITPSGDL T GRYNF GQL SPM+AHPKIDPD+GELFALSYNF HKPYLK+F 
Sbjct: 241 DLPYHVRITPSGDLITAGRYNFEGQLNSPMVAHPKIDPDTGELFALSYNFTHKPYLKYFQ 300

Query: 315 FSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKP 374
           FSPDGK+SPE+EIP+E PTMMHDFAITEN++VIPDQQV+FNL+KM+ GGSPVMYDEKK P
Sbjct: 301 FSPDGKISPEIEIPVEIPTMMHDFAITENFIVIPDQQVIFNLKKMLTGGSPVMYDEKKNP 360

Query: 375 RFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERDE 434
           RFGFL KN+ DSSNL WVDSP DTFCFHLWNAWEETES+E++VIGSCMTPPDSIFNERDE
Sbjct: 361 RFGFLLKNARDSSNLTWVDSPADTFCFHLWNAWEETESDEIIVIGSCMTPPDSIFNERDE 420

Query: 435 ELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKV 494
           E K VLTEIRFNL+TGES RR I+ ESE VNLE G+V+R ++GRKTR+ Y+AITEPWPK 
Sbjct: 421 EFKAVLTEIRFNLRTGESARRAIISESEDVNLEVGVVNRNRLGRKTRYVYLAITEPWPKA 480

Query: 495 SGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEEEGFIMAMVHDERRGKSEL 554
            G AKVDV SGE+KKY+YG++RYGGEP+ +G+      E+E++G+I+ +VHDE++ +SEL
Sbjct: 481 CGFAKVDVSSGEIKKYIYGDERYGGEPFFLGREMGCDCEREDDGYIVVLVHDEKKWRSEL 540

Query: 555 QIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
           QIVNA+DL++EA VELP+RVPYGFHGTF+ ANDL HQA
Sbjct: 541 QIVNAIDLKLEACVELPSRVPYGFHGTFVHANDLTHQA 577

BLAST of MS013473 vs. NCBI nr
Match: XP_023001406.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita maxima])

HSP 1 Score: 977.2 bits (2525), Expect = 6.0e-281
Identity = 467/602 (77.57%), Postives = 522/602 (86.71%), Query Frame = 0

Query: 2   ALSSSSSAK-----------VAVEMGLGMGFSARPINIGKKPNQKNEGISCALKSPSVFH 61
           ALSSSS++              V MG G+GF  RPI   KKPN +NE I+CAL SPSVFH
Sbjct: 4   ALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIR--KKPNLRNEVIACALNSPSVFH 63

Query: 62  FPEQPNKRPRITEEDSSRCRH-PPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPR 121
           +P+QPNKRPRITE+D+S+CRH PP WN LQKGASMA D+LEQALL N DN NLPK FDP 
Sbjct: 64  YPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPH 123

Query: 122 VQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAV 181
           VQIAGNYAPVPEQ V H LPVTG IPDCINGVYLRNGANPLF+PL GHHLFDGDGM+HAV
Sbjct: 124 VQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMIHAV 183

Query: 182 TIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDH 241
           TIGGG ASYACRFTETERL+QER+IGRP+FPK IGELHGHSGIARLLLF+ RGLFGLIDH
Sbjct: 184 TIGGGCASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLFGLIDH 243

Query: 242 RRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKI 301
           R GTGVANAGLVYFNGRLLAMSE+DLPYHVRITPSGDL T GRYNF G L SPM+AHPKI
Sbjct: 244 RHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMVAHPKI 303

Query: 302 DPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQ 361
           DPDSGELFALSYNF HKPYL HF FSPDGKMSP++EIP+E PTMMHDFAITEN+VVIPDQ
Sbjct: 304 DPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 363

Query: 362 QVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEET 421
           QVVFNLRKM+ GGSPVMYDEKK PRFGFLPKN+ DSSNLIWVDSP DTFCFHLWNAWEET
Sbjct: 364 QVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 423

Query: 422 ESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGM 481
           ESNEVVVIGSCMTPPDSIFN +DE+LK +LTEIRFNL TG+S RRPI+ +SE+VNLE GM
Sbjct: 424 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGM 483

Query: 482 VDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS--- 541
           V++  +GRKTR+AY+AITEPWPK  G AKVD+ SGE+KKY+YG+ RYGGEPY +G+    
Sbjct: 484 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 543

Query: 542 --EKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAH 586
             E+E++GFIM +VHDE++ +SELQIVNA+DL++EASVELPTRVPYGFHGTF+ A+ L H
Sbjct: 544 DCEREDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHASHLPH 602

BLAST of MS013473 vs. NCBI nr
Match: XP_022927145.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita moschata])

HSP 1 Score: 976.5 bits (2523), Expect = 1.0e-280
Identity = 464/591 (78.51%), Postives = 519/591 (87.82%), Query Frame = 0

Query: 2   ALSSSSSAKVAVEMGLGMGFSARPINIGKKPNQKNEGISCALKSPSVFHFPEQPNKRPRI 61
           ALSSSS+   ++ +GLG+GF  RPI   KKPN +NE ISC L SPSVFH+P+QPNKRPRI
Sbjct: 4   ALSSSSA---SLHLGLGLGFPTRPIR--KKPNLRNEVISCTLNSPSVFHYPKQPNKRPRI 63

Query: 62  TEEDSSRCR-HPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVP 121
           TE+D+S+CR HPP WN LQKGASMA D+LEQALL N DN NLPK FDP VQIAGNYAPVP
Sbjct: 64  TEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAGNYAPVP 123

Query: 122 EQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYAC 181
           EQ V H LPVTG IPDCINGVYLRNGANPLFEPL GHHLFDGDGM+HAVTIGGG  SYAC
Sbjct: 124 EQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCTSYAC 183

Query: 182 RFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGL 241
           RFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDHR GTGVANAGL
Sbjct: 184 RFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGL 243

Query: 242 VYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALS 301
           VYFNG LLAMSE+DLPYHVRITPSGDL T GRYNF GQL SPM+AHPKIDPDSGELFALS
Sbjct: 244 VYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALS 303

Query: 302 YNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVR 361
           YNF HKPYL HF FSPDGKMSP++EIP+E PTMMHDFAITEN+VVIPDQQVVFNLRKM+ 
Sbjct: 304 YNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMIN 363

Query: 362 GGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVVVIGSC 421
           GGSPVMYD+KK PRFGFLPKN+ DSSNLIWVDSP DTFCFHLWNAWEETESNEVVVIGSC
Sbjct: 364 GGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSC 423

Query: 422 MTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTR 481
           MTPPDSIFN +DE+LK +LTEIRFNL TG+S RRPI+ + E+VNLE GMV++  +GRKTR
Sbjct: 424 MTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTR 483

Query: 482 FAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEEEGFIM 541
           +AY+AITEPWPK  G AKVD+ SGE+KKY+YG+ RYGGEPY +G+      E+E++GFIM
Sbjct: 484 YAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIM 543

Query: 542 AMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
            +VHDE++ +SELQIVNA+DL++EASVELP RVPYGFHGTF+ A+ L HQA
Sbjct: 544 VLVHDEKKWRSELQIVNAIDLKLEASVELPNRVPYGFHGTFVHASQLPHQA 588

BLAST of MS013473 vs. NCBI nr
Match: XP_023520051.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974.5 bits (2518), Expect = 3.9e-280
Identity = 465/591 (78.68%), Postives = 519/591 (87.82%), Query Frame = 0

Query: 2   ALSSSSSAKVAVEMGLGMGFSARPINIGKKPNQKNEGISCALKSPSVFHFPEQPNKRPRI 61
           ALSSSS++     +G G+GF  RPI   KKPN +NE ISCAL SPSVFH+P+QP+KRPRI
Sbjct: 4   ALSSSSASP---HLGFGLGFPTRPIR--KKPNLRNEVISCALNSPSVFHYPKQPSKRPRI 63

Query: 62  TEEDSSRCR-HPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVP 121
           TE+D+S+CR HPP WN LQKGASMA D+LEQALL N DN NLPK FDP VQIAGNYAPVP
Sbjct: 64  TEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAGNYAPVP 123

Query: 122 EQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYAC 181
           EQ V H LPVTG IPDCINGVYLRNGANPLFEPL GHHLFDGDGM+HAVTIGGG ASYAC
Sbjct: 124 EQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCASYAC 183

Query: 182 RFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGL 241
           RFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDHR GTGVANAGL
Sbjct: 184 RFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGL 243

Query: 242 VYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALS 301
           VYFNGRLLAMSE+DLPYHVRITPSGDL T GRYNF GQL SPM+AHPKIDPDSGELFALS
Sbjct: 244 VYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALS 303

Query: 302 YNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVR 361
           YNF HKPYL HF FSPDGKMSP++EIP+E PTMMHDFAITEN+VVIPDQQ+VFNLRKM+ 
Sbjct: 304 YNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQIVFNLRKMIN 363

Query: 362 GGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVVVIGSC 421
           GGSPVMYDEKK PRFGFLPKN+ DSSNLIWVDSP DTFCFHLWNAWEETESNEVVVIGSC
Sbjct: 364 GGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSC 423

Query: 422 MTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTR 481
           MTPPDSIFN +DE+LK +LTEIRFNL TG+S RRPI+ +SE+VNLE GMV++  +GRKTR
Sbjct: 424 MTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGMVNQSLVGRKTR 483

Query: 482 FAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEEEGFIM 541
           +AY+AITEPWPK  G AKVD+ SGE+KKY+YG+  YGGEPY +G+      E+E++GFIM
Sbjct: 484 YAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQLYGGEPYFLGRDVYSDCEREDDGFIM 543

Query: 542 AMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
             VHDE++ +SELQIVNA+DL++EASVELPTRVPYGFHGTF+ A+ L  QA
Sbjct: 544 VFVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHASHLPDQA 588

BLAST of MS013473 vs. NCBI nr
Match: XP_008446856.1 (PREDICTED: probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucumis melo] >KAA0053987.1 putative 9-cis-epoxycarotenoid dioxygenase NCED5 [Cucumis melo var. makuwa])

HSP 1 Score: 944.1 bits (2439), Expect = 5.7e-271
Identity = 448/596 (75.17%), Postives = 510/596 (85.57%), Query Frame = 0

Query: 4   SSSSSAKVAVE-------MGLGMGFSARPINI-GKKPNQKNEGISCALKSPSVFHFPEQP 63
           SSS+S    +E       +GLG+GF   PINI  KKPNQ+ E IS AL SP VFHFP+Q 
Sbjct: 5   SSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQT 64

Query: 64  NKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGN 123
           NK PRITE  +S+CRHP HWN LQK AS A D+LEQ  L NNDN+NLPK FDP  QI GN
Sbjct: 65  NKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVGN 124

Query: 124 YAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGR 183
           YAPVPEQPV H LPVTG IPD INGVYLRNGANP F+PLAGHHLFDGDGM+HAVTIGGGR
Sbjct: 125 YAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGGR 184

Query: 184 ASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGV 243
           ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDHRRGTGV
Sbjct: 185 ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRGTGV 244

Query: 244 ANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGE 303
           ANAGLVYFNGRLLAMSEDDLPY VRITPSGDL TVGRYNF GQL SPM+AHPKIDP+SGE
Sbjct: 245 ANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPESGE 304

Query: 304 LFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNL 363
           LFALSYNF HKPYLKHF  +PDGKMSP+++IP++ PTM+HDFAITE +VVIPDQQVVFNL
Sbjct: 305 LFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVVFNL 364

Query: 364 RKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVV 423
           +KM+ GGSPVMYDEKK PRFGFLPKN+ DSSNL WVDSP DTFCFHLWNAWEETES++VV
Sbjct: 365 QKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDVV 424

Query: 424 VIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQI 483
           VI SCMTPPDSIFNERDEE K VLTEIRFNL+TG+S+ RPI+ ESE +NLE G+V+R ++
Sbjct: 425 VIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNRKRL 484

Query: 484 GRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEE 543
           GRKT++ Y+AIT+PWPK  G AKVDV SGE+KKY+YG++RYGGEPY +G+      E+E+
Sbjct: 485 GRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEED 544

Query: 544 EGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
           +G+IM  VHDE+  +SELQIVNA+DL++EA VEL +RVPYGFHGTF+ ANDL HQA
Sbjct: 545 DGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

BLAST of MS013473 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 1.5e-234
Identity = 377/514 (73.35%), Postives = 445/514 (86.58%), Query Frame = 0

Query: 74  HWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGT 133
           HWNF QK A+ ALD++E AL+S      LPK  DPRVQIAGN+APVPEQ V H LPVTGT
Sbjct: 67  HWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGT 126

Query: 134 IPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQERA 193
           IPDCINGVY+RNGANPLFEP+AGHHLFDGDGMVHAVT+  G  SY+CRFTETERL+QER 
Sbjct: 127 IPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERLVQERE 186

Query: 194 IGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSED 253
           +G PVFPKAIGELHGHSGIARLLLF+ RG+FGL+DH  GTGVANAGLV+FN RLLAMSED
Sbjct: 187 LGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSED 246

Query: 254 DLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFW 313
           D+PYHV++ PSGDL+TVGRYNF+ QL+S MIAHPKIDP SGELFALSY+ V KPYLK F 
Sbjct: 247 DVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFK 306

Query: 314 FSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKP 373
           FSPDG+ SP+VEIP++ PTMMHDFAITENYVVIPDQQVVF L++M++GGSPV+YD+ KK 
Sbjct: 307 FSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKS 366

Query: 374 RFGFLPKNSPDSSNLIWVDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEE 433
           RFG LPKN+ +S N+IWV+S +TFCFHLWNAWEE E++EV+VIGSCMTPPDSIFNE +E 
Sbjct: 367 RFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNEN 426

Query: 434 LKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVS 493
           LK VL+EIR NLKTGESTRR +L  S+QVNLEAGMV+R ++GRKT+FAY+AI EPWPKVS
Sbjct: 427 LKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVS 486

Query: 494 GIAKVDVRSGEMKKYVYGEDRYGGEPYLMGK---SEKEEEGFIMAMVHDERRGKSELQIV 553
           G AKVD+ +GE+KK++YG+ RYGGEP  + +   SEKE++G+I+A  HDE+  KSELQIV
Sbjct: 487 GFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTWKSELQIV 546

Query: 554 NAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQ 585
           NAM LE+EA+V+LP+RVPYGFHGTFI + DL +Q
Sbjct: 547 NAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

BLAST of MS013473 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 792.3 bits (2045), Expect = 3.6e-228
Identity = 377/590 (63.90%), Postives = 476/590 (80.68%), Query Frame = 0

Query: 10  KVAVEMGLGMGFSARPINIGK-KPNQKNEGISCALKSPSVFHFPEQPN-----KRPRIT- 69
           K+++      GF++  I++ K + N+++  I+ +L++P + HFP+Q +     K   I+ 
Sbjct: 16  KLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISH 75

Query: 70  ---EEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPV 129
              E ++S       WN +QK A+MALD +E AL  +     LPK  DPRVQI+GN+APV
Sbjct: 76  PKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPV 135

Query: 130 PEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYA 189
           PE PVC  LPVTG IP C+ GVY+RNGANPLFEP AGHH FDGDGMVHAV    G ASYA
Sbjct: 136 PENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYA 195

Query: 190 CRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAG 249
           CRFTETERL+QE+A+GRPVFPKAIGELHGHSGIARL+LF+ RGLFGL+DH +GTGVANAG
Sbjct: 196 CRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAG 255

Query: 250 LVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFAL 309
           LVYFN RLLAMSEDDLPYHV++TP+GDL+T GR++F+GQL+S MIAHPK+DP SGELFAL
Sbjct: 256 LVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFAL 315

Query: 310 SYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMV 369
           SY+ + KPYLK+F FS +G+ S +VEIP+E PTMMHDFAITEN+VVIPDQQVVF + +M+
Sbjct: 316 SYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMI 375

Query: 370 RGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSPDTFCFHLWNAWEETESNEVVVIGSC 429
           RGGSPV+YD+ K  RFG L K + D S+L WV+ PD FCFHLWNAWEE E++E+VVIGSC
Sbjct: 376 RGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSC 435

Query: 430 MTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPE-SEQVNLEAGMVDRGQIGRKT 489
           MTPPDSIFNE DE LK VL+EIR NLKTG+STR+ I+    EQVNLEAGMV+R ++GRKT
Sbjct: 436 MTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKT 495

Query: 490 RFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGK---SEKEEEGFIMA 549
            +AY+AI EPWPKVSG AKV++ +GE++K++YG+++YGGEP  + +   S++E++G+I+A
Sbjct: 496 EYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILA 555

Query: 550 MVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
            VHDE+  KSELQIVNAM L++EA+V+LP+RVPYGFHGTFI+ANDLA+QA
Sbjct: 556 FVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of MS013473 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.0e-222
Identity = 365/548 (66.61%), Postives = 443/548 (80.84%), Query Frame = 0

Query: 39  ISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNND 98
           +S AL +P   HFP+Q +  P I  +  ++  +    N  Q+ A+ ALD  E  L+S+  
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 99  NRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHH 158
              LPK  DP VQIAGN+APV EQPV   LPV G +PD I GVY+RNGANPL EP+ GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 159 LFDGDGMVHAVTIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLF 218
            FDGDGMVHAV    G ASYACRFT+T R +QER +GRPVFPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 219 FTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQ 278
           + R   G++D   GTGVANAGLVYFNGRLLAMSEDDLPY V+ITP+GDL+TVGR++F+GQ
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 279 LQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFA 338
           L+S MIAHPK+DP+SGELFALSY+ V KPYLK+F FSPDG  SP+VEI ++ PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 339 ITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSPDTFC 398
           ITEN+VV+PDQQVVF L +M+RGGSPV+YD+ K  RFG L K + DSSN+ W+D+PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 399 FHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILP- 458
           FHLWNAWEE E++EVVVIGSCMTPPDSIFNE DE LK VL+EIR NLKTGESTRRPI+  
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 459 ESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGG 518
           E +QVNLEAGMV+R  +GRKT+FAY+A+ EPWPKVSG AKVD+ +GE+KK++YG++RYGG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 519 EP-YLMGKSEKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFI 578
           EP +L G+  +E+EG+I+  VHDE+  KSELQIVNA+ LE+EA+V+LP+RVPYGFHGTFI
Sbjct: 530 EPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFI 589

Query: 579 DANDLAHQ 585
            A+DLA Q
Sbjct: 590 GADDLAKQ 597

BLAST of MS013473 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 5.6e-221
Identity = 363/558 (65.05%), Postives = 449/558 (80.47%), Query Frame = 0

Query: 31  KPNQKNEGISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLE 90
           KP ++    +    +P++ +FP  P+  P I E+D+SR      WN LQ+ AS ALD  E
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKDTSR------WNPLQRAASAALDFAE 97

Query: 91  QALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPL 150
            ALL    ++ LPK  DPR QI+GNYAPVPEQ V   L V G IPDCI+GVYLRNGANPL
Sbjct: 98  TALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPL 157

Query: 151 FEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHS 210
           FEP++GHHLFDGDGMVHAV I  G ASY+CRFTETERL+QE+ +G P+FPKAIGELHGHS
Sbjct: 158 FEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHS 217

Query: 211 GIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETV 270
           GIARL+LF+ RGLFGL++H+ GTGVANAGLVYF+ RLLAMSEDDLPY VR+T +GDLET+
Sbjct: 218 GIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETI 277

Query: 271 GRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMET 330
           GR++F+GQL S MIAHPKIDP + ELFALSY+ V KPYLK+F FSP+G+ SP+VEIP+ +
Sbjct: 278 GRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLAS 337

Query: 331 PTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIW 390
           PTMMHDFAITEN+VVIPDQQVVF L  M  G SPV YD +K  RFG LP+N+ D+S ++W
Sbjct: 338 PTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVW 397

Query: 391 VDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGES 450
           V+SP+TFCFHLWNAWE  E++EVVVIGSCMTP DSIFNE DE+L  VL+EIR NLKTG+S
Sbjct: 398 VESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKS 457

Query: 451 TRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVY 510
           TRR I+P S Q+NLEAGMV+R  +GRKTR+AY+AI EPWPKVSG AKVD+ +GE+K + Y
Sbjct: 458 TRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFY 517

Query: 511 GEDRYGGEPYLMGK---SEKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRV 570
           G  +YGGEP+ + +   S+ E++G+IM+ VHDE   +SEL IVNA+ LE+EA+V+LP+RV
Sbjct: 518 GGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRV 577

Query: 571 PYGFHGTFIDANDLAHQA 586
           PYGFHGTF+++ D+ +QA
Sbjct: 578 PYGFHGTFVNSADMLNQA 589

BLAST of MS013473 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 3.1e-219
Identity = 352/514 (68.48%), Postives = 426/514 (82.88%), Query Frame = 0

Query: 72  PPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVT 131
           P  WNFLQK A+  LDM+E AL+S+     LPK  DP+VQIAGN+APVPE      LPV 
Sbjct: 102 PQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVV 161

Query: 132 GTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQE 191
           G IP CI+GVY+RNGANPL+EP+AGHH FDGDGMVHAV    G ASYACRFTET+RL QE
Sbjct: 162 GKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQRLAQE 221

Query: 192 RAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMS 251
           +++GRPVFPKAIGELHGHSGIARLLLF+ R LF L+D   G GVANAGLVYFN  LLAMS
Sbjct: 222 KSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMS 281

Query: 252 EDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKH 311
           EDDLPYHVRIT +GDL TVGRY+FNGQL S MIAHPK+DP +G+L ALSY+ V KPYLK+
Sbjct: 282 EDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKY 341

Query: 312 FWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKK 371
           F FS DG  SP+VEIP++ PTMMHDFAITEN+VV+PDQQVVF L +M+ GGSPV+YD+ K
Sbjct: 342 FRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNK 401

Query: 372 KPRFGFLPKNSPDSSNLIWVDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERD 431
             RFG L KN+ D++ + W+D+P+ FCFHLWNAWEE E++E+VVIGSCMTP DSIFNE D
Sbjct: 402 TSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECD 461

Query: 432 EELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPK 491
           E LK VL+EIR NL+TG+STRRPI+ ++EQVNLEAGMV+R ++GRKT+FAY+A+ EPWPK
Sbjct: 462 ESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPK 521

Query: 492 VSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKSEKEEEGFIMAMVHDERRGKSELQIVN 551
           VSG AKVD+ SGE++KY+YGE+++GGEP  +   E+E +G+I+A VHDE+  KSELQIVN
Sbjct: 522 VSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLPNGEEEGDGYILAFVHDEKEWKSELQIVN 581

Query: 552 AMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
           A +L++EAS++LP+RVPYGFHGTFI + DL  QA
Sbjct: 582 AQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

BLAST of MS013473 vs. ExPASy TrEMBL
Match: A0A6J1KII7 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495550 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.9e-281
Identity = 467/602 (77.57%), Postives = 522/602 (86.71%), Query Frame = 0

Query: 2   ALSSSSSAK-----------VAVEMGLGMGFSARPINIGKKPNQKNEGISCALKSPSVFH 61
           ALSSSS++              V MG G+GF  RPI   KKPN +NE I+CAL SPSVFH
Sbjct: 4   ALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIR--KKPNLRNEVIACALNSPSVFH 63

Query: 62  FPEQPNKRPRITEEDSSRCRH-PPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPR 121
           +P+QPNKRPRITE+D+S+CRH PP WN LQKGASMA D+LEQALL N DN NLPK FDP 
Sbjct: 64  YPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPH 123

Query: 122 VQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAV 181
           VQIAGNYAPVPEQ V H LPVTG IPDCINGVYLRNGANPLF+PL GHHLFDGDGM+HAV
Sbjct: 124 VQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMIHAV 183

Query: 182 TIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDH 241
           TIGGG ASYACRFTETERL+QER+IGRP+FPK IGELHGHSGIARLLLF+ RGLFGLIDH
Sbjct: 184 TIGGGCASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLFGLIDH 243

Query: 242 RRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKI 301
           R GTGVANAGLVYFNGRLLAMSE+DLPYHVRITPSGDL T GRYNF G L SPM+AHPKI
Sbjct: 244 RHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMVAHPKI 303

Query: 302 DPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQ 361
           DPDSGELFALSYNF HKPYL HF FSPDGKMSP++EIP+E PTMMHDFAITEN+VVIPDQ
Sbjct: 304 DPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 363

Query: 362 QVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEET 421
           QVVFNLRKM+ GGSPVMYDEKK PRFGFLPKN+ DSSNLIWVDSP DTFCFHLWNAWEET
Sbjct: 364 QVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 423

Query: 422 ESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGM 481
           ESNEVVVIGSCMTPPDSIFN +DE+LK +LTEIRFNL TG+S RRPI+ +SE+VNLE GM
Sbjct: 424 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGM 483

Query: 482 VDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS--- 541
           V++  +GRKTR+AY+AITEPWPK  G AKVD+ SGE+KKY+YG+ RYGGEPY +G+    
Sbjct: 484 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 543

Query: 542 --EKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAH 586
             E+E++GFIM +VHDE++ +SELQIVNA+DL++EASVELPTRVPYGFHGTF+ A+ L H
Sbjct: 544 DCEREDDGFIMVLVHDEKKWRSELQIVNAIDLKLEASVELPTRVPYGFHGTFVHASHLPH 602

BLAST of MS013473 vs. ExPASy TrEMBL
Match: A0A6J1EN25 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111434081 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 5.0e-281
Identity = 464/591 (78.51%), Postives = 519/591 (87.82%), Query Frame = 0

Query: 2   ALSSSSSAKVAVEMGLGMGFSARPINIGKKPNQKNEGISCALKSPSVFHFPEQPNKRPRI 61
           ALSSSS+   ++ +GLG+GF  RPI   KKPN +NE ISC L SPSVFH+P+QPNKRPRI
Sbjct: 4   ALSSSSA---SLHLGLGLGFPTRPIR--KKPNLRNEVISCTLNSPSVFHYPKQPNKRPRI 63

Query: 62  TEEDSSRCR-HPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVP 121
           TE+D+S+CR HPP WN LQKGASMA D+LEQALL N DN NLPK FDP VQIAGNYAPVP
Sbjct: 64  TEDDASKCRHHPPRWNLLQKGASMAFDILEQALL-NYDNLNLPKNFDPHVQIAGNYAPVP 123

Query: 122 EQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYAC 181
           EQ V H LPVTG IPDCINGVYLRNGANPLFEPL GHHLFDGDGM+HAVTIGGG  SYAC
Sbjct: 124 EQSVSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCTSYAC 183

Query: 182 RFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGL 241
           RFTETERL+QER+IGRPVFPK IGELHGHSGIARLLLF+ RGLFGLIDHR GTGVANAGL
Sbjct: 184 RFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGL 243

Query: 242 VYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALS 301
           VYFNG LLAMSE+DLPYHVRITPSGDL T GRYNF GQL SPM+AHPKIDPDSGELFALS
Sbjct: 244 VYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALS 303

Query: 302 YNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVR 361
           YNF HKPYL HF FSPDGKMSP++EIP+E PTMMHDFAITEN+VVIPDQQVVFNLRKM+ 
Sbjct: 304 YNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMIN 363

Query: 362 GGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVVVIGSC 421
           GGSPVMYD+KK PRFGFLPKN+ DSSNLIWVDSP DTFCFHLWNAWEETESNEVVVIGSC
Sbjct: 364 GGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSC 423

Query: 422 MTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTR 481
           MTPPDSIFN +DE+LK +LTEIRFNL TG+S RRPI+ + E+VNLE GMV++  +GRKTR
Sbjct: 424 MTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTR 483

Query: 482 FAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEEEGFIM 541
           +AY+AITEPWPK  G AKVD+ SGE+KKY+YG+ RYGGEPY +G+      E+E++GFIM
Sbjct: 484 YAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIM 543

Query: 542 AMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
            +VHDE++ +SELQIVNA+DL++EASVELP RVPYGFHGTF+ A+ L HQA
Sbjct: 544 VLVHDEKKWRSELQIVNAIDLKLEASVELPNRVPYGFHGTFVHASQLPHQA 588

BLAST of MS013473 vs. ExPASy TrEMBL
Match: A0A5A7UHG6 (Putative 9-cis-epoxycarotenoid dioxygenase NCED5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00330 PE=3 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 448/596 (75.17%), Postives = 510/596 (85.57%), Query Frame = 0

Query: 4   SSSSSAKVAVE-------MGLGMGFSARPINI-GKKPNQKNEGISCALKSPSVFHFPEQP 63
           SSS+S    +E       +GLG+GF   PINI  KKPNQ+ E IS AL SP VFHFP+Q 
Sbjct: 5   SSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQT 64

Query: 64  NKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGN 123
           NK PRITE  +S+CRHP HWN LQK AS A D+LEQ  L NNDN+NLPK FDP  QI GN
Sbjct: 65  NKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVGN 124

Query: 124 YAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGR 183
           YAPVPEQPV H LPVTG IPD INGVYLRNGANP F+PLAGHHLFDGDGM+HAVTIGGGR
Sbjct: 125 YAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGGR 184

Query: 184 ASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGV 243
           ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDHRRGTGV
Sbjct: 185 ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRGTGV 244

Query: 244 ANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGE 303
           ANAGLVYFNGRLLAMSEDDLPY VRITPSGDL TVGRYNF GQL SPM+AHPKIDP+SGE
Sbjct: 245 ANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPESGE 304

Query: 304 LFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNL 363
           LFALSYNF HKPYLKHF  +PDGKMSP+++IP++ PTM+HDFAITE +VVIPDQQVVFNL
Sbjct: 305 LFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVVFNL 364

Query: 364 RKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVV 423
           +KM+ GGSPVMYDEKK PRFGFLPKN+ DSSNL WVDSP DTFCFHLWNAWEETES++VV
Sbjct: 365 QKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDVV 424

Query: 424 VIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQI 483
           VI SCMTPPDSIFNERDEE K VLTEIRFNL+TG+S+ RPI+ ESE +NLE G+V+R ++
Sbjct: 425 VIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNRKRL 484

Query: 484 GRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEE 543
           GRKT++ Y+AIT+PWPK  G AKVDV SGE+KKY+YG++RYGGEPY +G+      E+E+
Sbjct: 485 GRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEED 544

Query: 544 EGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
           +G+IM  VHDE+  +SELQIVNA+DL++EA VEL +RVPYGFHGTF+ ANDL HQA
Sbjct: 545 DGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

BLAST of MS013473 vs. ExPASy TrEMBL
Match: A0A1S3BG38 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489449 PE=3 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 448/596 (75.17%), Postives = 510/596 (85.57%), Query Frame = 0

Query: 4   SSSSSAKVAVE-------MGLGMGFSARPINI-GKKPNQKNEGISCALKSPSVFHFPEQP 63
           SSS+S    +E       +GLG+GF   PINI  KKPNQ+ E IS AL SP VFHFP+Q 
Sbjct: 5   SSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHFPKQT 64

Query: 64  NKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGN 123
           NK PRITE  +S+CRHP HWN LQK AS A D+LEQ  L NNDN+NLPK FDP  QI GN
Sbjct: 65  NKPPRITEHHTSKCRHPSHWNLLQKVASTAFDILEQTFL-NNDNQNLPKNFDPHFQIVGN 124

Query: 124 YAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGR 183
           YAPVPEQPV H LPVTG IPD INGVYLRNGANP F+PLAGHHLFDGDGM+HAVTIGGGR
Sbjct: 125 YAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTIGGGR 184

Query: 184 ASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGV 243
           ASYACRFTETERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RG FGLIDHRRGTGV
Sbjct: 185 ASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRRGTGV 244

Query: 244 ANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGE 303
           ANAGLVYFNGRLLAMSEDDLPY VRITPSGDL TVGRYNF GQL SPM+AHPKIDP+SGE
Sbjct: 245 ANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDPESGE 304

Query: 304 LFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNL 363
           LFALSYNF HKPYLKHF  +PDGKMSP+++IP++ PTM+HDFAITE +VVIPDQQVVFNL
Sbjct: 305 LFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQVVFNL 364

Query: 364 RKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVV 423
           +KM+ GGSPVMYDEKK PRFGFLPKN+ DSSNL WVDSP DTFCFHLWNAWEETES++VV
Sbjct: 365 QKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDVV 424

Query: 424 VIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQI 483
           VI SCMTPPDSIFNERDEE K VLTEIRFNL+TG+S+ RPI+ ESE +NLE G+V+R ++
Sbjct: 425 VIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVNRKRL 484

Query: 484 GRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEE 543
           GRKT++ Y+AIT+PWPK  G AKVDV SGE+KKY+YG++RYGGEPY +G+      E+E+
Sbjct: 485 GRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEED 544

Query: 544 EGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
           +G+IM  VHDE+  +SELQIVNA+DL++EA VEL +RVPYGFHGTF+ ANDL HQA
Sbjct: 545 DGYIMVFVHDEKEWRSELQIVNAIDLKLEACVELKSRVPYGFHGTFVHANDLRHQA 599

BLAST of MS013473 vs. ExPASy TrEMBL
Match: A0A0A0M030 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435760 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.5e-266
Identity = 442/591 (74.79%), Postives = 505/591 (85.45%), Query Frame = 0

Query: 4   SSSSSAKVAVEMGLGMGFSARPINI-GKKPNQKNEGISCALK--SPSVFHFPEQPNKRPR 63
           SS+S++   V    G+GF  +PINI  KKPNQ+ E IS ALK  SP VFHFP+QPNK PR
Sbjct: 4   SSTSTSPHFVLDQKGLGFPIKPINIVKKKPNQRKEVISSALKVNSPFVFHFPKQPNKGPR 63

Query: 64  ITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVP 123
           ITE  + + RHP HWN LQK AS A D+LEQA L NN NRNLPK FDP  QI GNYAPVP
Sbjct: 64  ITEHYTFKSRHPSHWNLLQKVASTAFDILEQAFL-NNHNRNLPKNFDPHFQIVGNYAPVP 123

Query: 124 EQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYAC 183
           EQPV H LPVTG IP  INGVYLRNGANP F+PL+GHHLFDGDGM+HAVTIG GRASYAC
Sbjct: 124 EQPVSHSLPVTGVIPHWINGVYLRNGANPFFQPLSGHHLFDGDGMIHAVTIGEGRASYAC 183

Query: 184 RFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGL 243
           RFT+TERL+QE++IGRPVFPKA+GELHGHSGIARLLLF+ RGLFGLIDHRRGTGVANAGL
Sbjct: 184 RFTQTERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGLFGLIDHRRGTGVANAGL 243

Query: 244 VYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALS 303
           VYFNGRLLAMSEDDLPY VRITPSGDL T+GRYNF GQL SPM+AHPKIDPDSGELFALS
Sbjct: 244 VYFNGRLLAMSEDDLPYQVRITPSGDLITIGRYNFEGQLNSPMVAHPKIDPDSGELFALS 303

Query: 304 YNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVR 363
           YNF HKPYLKHF  +PDGKMSPE++IP+E PTM+HDFAITE +VVIPDQQVVFNL+KM+ 
Sbjct: 304 YNFTHKPYLKHFRLTPDGKMSPEIDIPLEIPTMIHDFAITEKFVVIPDQQVVFNLQKMLS 363

Query: 364 GGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSP-DTFCFHLWNAWEETESNEVVVIGSC 423
           GGSPV+YDEKK PRFGFLPKN+ DSSNL WVDSP DTFCFHLWNAWEETES+++VVI SC
Sbjct: 364 GGSPVVYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDIVVIASC 423

Query: 424 MTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTR 483
           MTPPDSIFNERDEE K VLTEIRFNL+TG+S+ R I+ ESE VNLE G+V+R ++GRKT+
Sbjct: 424 MTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRRIISESEHVNLEVGVVNRKRVGRKTQ 483

Query: 484 FAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKS-----EKEEEGFIM 543
           + Y+AIT+PWPK  G AKVDV SGE+KKY+YG++RYGGEPY +G+      E+E++G IM
Sbjct: 484 YVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEEDDGHIM 543

Query: 544 AMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQA 586
             VHDE++ +SELQ+VNA DL++EA VEL +RVPYGFHGTF+ ANDL HQA
Sbjct: 544 VFVHDEKKWRSELQVVNATDLKLEACVELSSRVPYGFHGTFVHANDLIHQA 593

BLAST of MS013473 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 774.2 bits (1998), Expect = 7.2e-224
Identity = 365/548 (66.61%), Postives = 443/548 (80.84%), Query Frame = 0

Query: 39  ISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLEQALLSNND 98
           +S AL +P   HFP+Q +  P I  +  ++  +    N  Q+ A+ ALD  E  L+S+  
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 99  NRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPLFEPLAGHH 158
              LPK  DP VQIAGN+APV EQPV   LPV G +PD I GVY+RNGANPL EP+ GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 159 LFDGDGMVHAVTIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHSGIARLLLF 218
            FDGDGMVHAV    G ASYACRFT+T R +QER +GRPVFPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 219 FTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETVGRYNFNGQ 278
           + R   G++D   GTGVANAGLVYFNGRLLAMSEDDLPY V+ITP+GDL+TVGR++F+GQ
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 279 LQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMETPTMMHDFA 338
           L+S MIAHPK+DP+SGELFALSY+ V KPYLK+F FSPDG  SP+VEI ++ PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 339 ITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIWVDSPDTFC 398
           ITEN+VV+PDQQVVF L +M+RGGSPV+YD+ K  RFG L K + DSSN+ W+D+PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 399 FHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGESTRRPILP- 458
           FHLWNAWEE E++EVVVIGSCMTPPDSIFNE DE LK VL+EIR NLKTGESTRRPI+  
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 459 ESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVYGEDRYGG 518
           E +QVNLEAGMV+R  +GRKT+FAY+A+ EPWPKVSG AKVD+ +GE+KK++YG++RYGG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 519 EP-YLMGKSEKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRVPYGFHGTFI 578
           EP +L G+  +E+EG+I+  VHDE+  KSELQIVNA+ LE+EA+V+LP+RVPYGFHGTFI
Sbjct: 530 EPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFI 589

Query: 579 DANDLAHQ 585
            A+DLA Q
Sbjct: 590 GADDLAKQ 597

BLAST of MS013473 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 768.5 bits (1983), Expect = 4.0e-222
Identity = 363/558 (65.05%), Postives = 449/558 (80.47%), Query Frame = 0

Query: 31  KPNQKNEGISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPPHWNFLQKGASMALDMLE 90
           KP ++    +    +P++ +FP  P+  P I E+D+SR      WN LQ+ AS ALD  E
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKDTSR------WNPLQRAASAALDFAE 97

Query: 91  QALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYLRNGANPL 150
            ALL    ++ LPK  DPR QI+GNYAPVPEQ V   L V G IPDCI+GVYLRNGANPL
Sbjct: 98  TALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPL 157

Query: 151 FEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQERAIGRPVFPKAIGELHGHS 210
           FEP++GHHLFDGDGMVHAV I  G ASY+CRFTETERL+QE+ +G P+FPKAIGELHGHS
Sbjct: 158 FEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHS 217

Query: 211 GIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITPSGDLETV 270
           GIARL+LF+ RGLFGL++H+ GTGVANAGLVYF+ RLLAMSEDDLPY VR+T +GDLET+
Sbjct: 218 GIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETI 277

Query: 271 GRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPEVEIPMET 330
           GR++F+GQL S MIAHPKIDP + ELFALSY+ V KPYLK+F FSP+G+ SP+VEIP+ +
Sbjct: 278 GRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLAS 337

Query: 331 PTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSPDSSNLIW 390
           PTMMHDFAITEN+VVIPDQQVVF L  M  G SPV YD +K  RFG LP+N+ D+S ++W
Sbjct: 338 PTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVW 397

Query: 391 VDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRFNLKTGES 450
           V+SP+TFCFHLWNAWE  E++EVVVIGSCMTP DSIFNE DE+L  VL+EIR NLKTG+S
Sbjct: 398 VESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKS 457

Query: 451 TRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSGEMKKYVY 510
           TRR I+P S Q+NLEAGMV+R  +GRKTR+AY+AI EPWPKVSG AKVD+ +GE+K + Y
Sbjct: 458 TRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFY 517

Query: 511 GEDRYGGEPYLMGK---SEKEEEGFIMAMVHDERRGKSELQIVNAMDLEMEASVELPTRV 570
           G  +YGGEP+ + +   S+ E++G+IM+ VHDE   +SEL IVNA+ LE+EA+V+LP+RV
Sbjct: 518 GGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRV 577

Query: 571 PYGFHGTFIDANDLAHQA 586
           PYGFHGTF+++ D+ +QA
Sbjct: 578 PYGFHGTFVNSADMLNQA 589

BLAST of MS013473 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 760.8 bits (1963), Expect = 8.3e-220
Identity = 363/563 (64.48%), Postives = 450/563 (79.93%), Query Frame = 0

Query: 25  PINIGKKPNQKNEGISCALKSPSVFHFPEQPNKRPRITEEDSSRCRHPPHWNFLQKGASM 84
           P  +    N+K+  +S +L+SP+ F  P        +T          P  N +Q+ A+M
Sbjct: 97  PCYVSSSVNKKS-SVSSSLQSPT-FKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAM 156

Query: 85  ALDMLEQALLSNNDNRN-LPKIFDPRVQIAGNYAPVPEQPVCHELPVTGTIPDCINGVYL 144
            LD +E A++S+   R+  PK  DP VQIAGN+ PVPE+PV H LPVTGT+P+CI GVY+
Sbjct: 157 VLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYV 216

Query: 145 RNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQERAIGRPVFPKAI 204
           RNGANPL +P++GHHLFDGDGMVHAV    G  SYACRFTET RL+QER  GRPVFPKAI
Sbjct: 217 RNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAI 276

Query: 205 GELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAMSEDDLPYHVRITP 264
           GELHGH GIA+L+LF TRGLFGL+D   G GVANAGLVYFNG LLAMSEDDLPYHV++T 
Sbjct: 277 GELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQ 336

Query: 265 SGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLKHFWFSPDGKMSPE 324
           +GDLET GRY+F+GQL+S MIAHPKIDP++ ELFALSY+ V KPYLK+F F+ DG+ SP+
Sbjct: 337 TGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPD 396

Query: 325 VEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEKKKPRFGFLPKNSP 384
           VEIP++ PTM+HDFAITEN+VVIPDQQVVF L +M+RGGSPV+YDEKKK RFG L KN+ 
Sbjct: 397 VEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAK 456

Query: 385 DSSNLIWVDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNERDEELKIVLTEIRF 444
           D+S++ W++ PD FCFHLWN+WEE E++EVVVIGSCMTPPDSIFNE DE L+ VL+EIR 
Sbjct: 457 DASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRL 516

Query: 445 NLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWPKVSGIAKVDVRSG 504
           NLKTGESTRRP++  SEQVNLEAGMV+R  +GRKTR+AY+A+TEPWPKVSG AKVD+ +G
Sbjct: 517 NLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTG 576

Query: 505 EMKKYVYGEDRYGGEPYLMGKSEKEEE-GFIMAMVHDERRGKSELQIVNAMDLEMEASVE 564
           E++KY+YGE +YGGEP  +   + EE+ G+IM  VHDE + KSELQ++NA+++++EA+V 
Sbjct: 577 EIRKYIYGEGKYGGEPLFLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVT 636

Query: 565 LPTRVPYGFHGTFIDANDLAHQA 586
           LP+RVPYGFHGTFI   DL+ QA
Sbjct: 637 LPSRVPYGFHGTFISKEDLSKQA 655

BLAST of MS013473 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 732.3 bits (1889), Expect = 3.2e-211
Identity = 344/519 (66.28%), Postives = 429/519 (82.66%), Query Frame = 0

Query: 71  HPPHWNFLQKGASMALDMLEQALLSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPV 130
           +P   N  QK A++A+D  E+AL+S+  +  LPK  DPRVQIAGNY+PVPE  V   L V
Sbjct: 65  NPLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTV 124

Query: 131 TGTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIGGGRASYACRFTETERLIQ 190
            GTIPDCI+GVY+RNGANP+FEP AGHHLFDGDGMVHAV I  G ASYACRFT+TERL+Q
Sbjct: 125 EGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQ 184

Query: 191 ERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAM 250
           E+ +GRPVFPKAIGELHGHSGIARL+LF+ RGL GLI+++ G GVANAGLVYFN RLLAM
Sbjct: 185 EKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAM 244

Query: 251 SEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLK 310
           SEDDLPY ++IT +GDL+TVGRY+F+GQL+S MIAHPK+DP + EL ALSY+ V KPYLK
Sbjct: 245 SEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLK 304

Query: 311 HFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEK 370
           +F FSPDG  SPE+EIP+ETPTM+HDFAITEN+VVIPDQQVVF L +M+ G SPV++D +
Sbjct: 305 YFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGE 364

Query: 371 KKPRFGFLPKNSPDSSNLIWVDSPDTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNER 430
           K  R G +PK++ ++S +IWV+SP+TFCFHLWNAWE  E+ E+VVIGSCM+P DSIFNER
Sbjct: 365 KVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNER 424

Query: 431 DEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITEPWP 490
           DE L+ VL+EIR NL+T ++TRR +L  +E VNLE GMV+R ++GRKTRFA++AI  PWP
Sbjct: 425 DESLRSVLSEIRINLRTRKTTRRSLL-VNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWP 484

Query: 491 KVSGIAKVDVRSGEMKKYVYGEDRYGGEP-YLMGKS----EKEEEGFIMAMVHDERRGKS 550
           KVSG AKVD+ +GEMKKY+YG ++YGGEP +L G S    E E++G+I   VHDE    S
Sbjct: 485 KVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTS 544

Query: 551 ELQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQ 585
           ELQI+NA++L++EA+++LP+RVPYGFHGTF+D+N+L  Q
Sbjct: 545 ELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of MS013473 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 612.5 bits (1578), Expect = 3.6e-175
Identity = 293/518 (56.56%), Postives = 384/518 (74.13%), Query Frame = 0

Query: 73  PHWNFLQKGASMALDMLEQAL-LSNNDNRNLPKIFDPRVQIAGNYAPVPEQPVCHELPVT 132
           P+ N LQK A+  LD +E ++ +    NR LPK  DP VQ++GN+APV E PV + L V 
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 133 GTIPDCINGVYLRNGANPLFEPLAGHHLFDGDGMVHAVTIG-GGRASYACRFTETERLIQ 192
           G IP C+ GVY+RNGANP+F PLAGHHLFDGDGM+HAV+IG   + SY+CR+T+T RL+Q
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQ 180

Query: 193 ERAIGRPVFPKAIGELHGHSGIARLLLFFTRGLFGLIDHRRGTGVANAGLVYFNGRLLAM 252
           E A+GR VFPK IGELHGHSG+ARL LF  R   GL+D  RG GVANAG+V+FNGRLLAM
Sbjct: 181 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAM 240

Query: 253 SEDDLPYHVRITPSGDLETVGRYNFNGQLQSPMIAHPKIDPDSGELFALSYNFVHKPYLK 312
           SEDDLPY V+I   GDLET+GR+ F+ Q+ S +IAHPK+D  +G+L  LSYN + KP+L+
Sbjct: 241 SEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLR 300

Query: 313 HFWFSPDGKMSPEVEIPMETPTMMHDFAITENYVVIPDQQVVFNLRKMVRGGSPVMYDEK 372
           +  F+  GK + +VEI +  PTM+HDFAITEN+VVIPDQQ+VF L +M+RGGSPV+Y ++
Sbjct: 301 YLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKE 360

Query: 373 KKPRFGFLPKNSPDSSNLIWVDSPDTFCFHLWNAWEE-TESNE--VVVIGSCMTPPDSIF 432
           K  RFG L K     S++ WVD PD FCFHLWNAWEE TE  +  +VVIGSCM+PPD+IF
Sbjct: 361 KMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIF 420

Query: 433 NERDEELKIVLTEIRFNLKTGESTRRPILPESEQVNLEAGMVDRGQIGRKTRFAYMAITE 492
           +E  E  ++ L+EIR N++T ES R+ I+     VNLEAG ++R  +GRK++F Y+AI +
Sbjct: 421 SESGEPTRVELSEIRLNMRTKESNRKVIV---TGVNLEAGHINRSYVGRKSQFVYIAIAD 480

Query: 493 PWPKVSGIAKVDVRSGEMKKYVYGEDRYGGEPYLMGKSEKEEE-GFIMAMVHDERRGKSE 552
           PWPK SGIAKVD+++G + ++ YG  R+GGEP  + + E EE+ G++M  V DE + +SE
Sbjct: 481 PWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEEKDESE 540

Query: 553 LQIVNAMDLEMEASVELPTRVPYGFHGTFIDANDLAHQ 585
             +V+A D++  A+V LP RVPYGFHGTF+  N L  Q
Sbjct: 541 FVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575