MS013468 (gene) Bitter gourd (TR) v1

Overview


Name	MS013468
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protease Do-like 5, chloroplastic
Location	scaffold402: 1764650 .. 1767208 (-)
RNA-Seq Expression	MS013468
Synteny	MS013468

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTAGCCTCACTGGGAATTCATCTTCTTCCAATTCCAGCTCCCCCAAATTCTTCCCACAACTCTCTACCCTTCACTTCGCGAAGAGCCCTAGTTTTTGCCCCATCTGCTTTGATGGCTTCCCTCCTCGCTTTCCCTCTTCCCACTCACGCCGCTCTCCCCCAACTACAGGACCAGGTTCCACAAGAAGAAGATCGAGTCGTCGCTCTCTTTCAGGTCCCTCTCTTTCTCACTCACCATAACTGCAGAATTCTTCAGTTTCAATTGAAATTTAATCTTGCTTCTTTGTTTTCTGTTTTGCTCTGTTGGGTCCCAATTCCAAATGTAGGATGCTTCACCTTCTGTCGTTTACATTAAGGACCTTGAATTAGCTAAGAAACCCCAGAACTCCTCTGAAGAGGCCCTGCTCGTCGAGGATGAGAATGTCAAGGTCAAAGGGACTGGTTCGGGCTTTGTATGGGATAAATTTGGCCATATCGTATGCTCTTCTCTCCTTTAATTCTTACTTGCCTTCATTCTTTGTGACTGTCATTTTCATTTTATTGATTTTGGGTATCGGAAACATTTCTTTTTCTGTTCATTTCACAGAGGAATTTCATTAGGGCTTTGTGATTACTTGTGGCAGGTAACTAATTACCATGTTGTTTCCGCATTGGCTACTGATAACAGTGGATTGCAGCGTTGTAAGGTTTTGTTTATTTGTTACTGTCTTTTGAATTGTTATTTATGTCTTCATAATGGAAGATATATTATCAGTCTCTAGCCACTCCATTTGGCTTGCTTCTGTAGGTAAATTTAGTCGATGCTAAAGGAAATGGAATTTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTCAAGGTATAGAACCAAACCTTTTTTTTTTCTTTCCTAATTTTCTTTGAAGCTTCTTGTCTGCTCTGTCGTGTTCAAACTGGGTGCTGTCTACTCAATATAGAAGTTGAAATGATTTTGACATTTACAAAATCCCTCTTGAGATGGTGCCCATATATATTCTGTTTCGTTTTGTTCTAATTTAGCTCGAGACTCGAGGGGAATACCAGATTGAGTGAATCGTGTCTCGGTGATTCTCATCCTGTTTTTCCGTTCCTTATGTGATGTGTGAAAGCAGCAGATTGGGAGGAGTGGAAACTGAACCCTGTGAGCTATGGAGTGATGATGATAATATGTCATTTGTTTTCTATTCCATAAAACAGGCTTCACTGTTTGGACAATCTGTACTGTCAATCTCTTATACTATACCATGATTTGCTGAGTTTGAAGTTGCCATGTAACATCCAGGTGGAACTTGGAGGATGTGAACTAAAGCCCATCGTTCTCGGTACCTCTCGAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTAAGACATCAATAATGTTACTTTCTCCATTTAATTCAGAGGAAACCCTCTTTGGATTTTCTATTTCAAAGCTAAAACTAAATTGGATAGTGAAAATACTTCTCTCTTATAATAAACATATTTCTCATAGGAGATATGATATCTCTCATCTCACACACATTTATGTTTTATTTATCAAAGACTGGATGAAGTAAGAGTTTTTCTAAGTGTCACTGTCAAGTTTCCTACTCATTGATTGATGGTGTTCTTTACCAAGAAACACCTGCTGAGAGTCAGCATAGCGGATTGACTAAATAATCGAGAACTGTAAATCATTCAGGGTTTGAATAGCAGGTGATCAGTGGATTGGGTAGAGAAATTCCATCACCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTTCAGGTTCATGGTTCAACTACCTGAAATCAGATACCGCCATTTCTCAAACTTGTTTTTTCATGCCTTTTCTTCTGCCGCTTTCTAAAGATTTTCTACTTCCCATCATGTATATTGCAAGAGTGAGCCTTTTTTATTCTTGACACTAAAGCGCTGTTAAGTTCCTTGTACATATATGGCAGCTTATCTTGTTCCTTTCTAATCTGGCTTGATTTCATTACTGCCCCAGTCACCCGTAATAATCATCATCATATTTTAACTTTCCCACATTTTAATGCATTAGAGTTTTACAGGGAATTCAGGGGGGCCATTAATTGACTCCTACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGTAAAGGGCCTCAATTTCTTCATCTGCTTAGTTTGTCAGAGAAAAATATGGCTGATTTCTTTGCCTTAAAGCTTAGTAATTATCAAAGCTAACTCCATCTCTCTCTCTCTCTTTTATTTGCTACTTGAAAGAACATAAAATAGTAAAGAAAGAAAGAAAGGACTGCGGTTTTTTTGTGCCAATTCTATTCGAAGTTATATTATTCATCTTTGTTTATGCATTGCAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATTCCAATAGACACAGTTGTACGTACGGTTCCATACCTTATTGTATACGGAACACCTTACAGTGAGAGATTT

mRNA sequence

ATGGCGTTAGCCTCACTGGGAATTCATCTTCTTCCAATTCCAGCTCCCCCAAATTCTTCCCACAACTCTCTACCCTTCACTTCGCGAAGAGCCCTAGTTTTTGCCCCATCTGCTTTGATGGCTTCCCTCCTCGCTTTCCCTCTTCCCACTCACGCCGCTCTCCCCCAACTACAGGACCAGGTTCCACAAGAAGAAGATCGAGTCGTCGCTCTCTTTCAGGATGCTTCACCTTCTGTCGTTTACATTAAGGACCTTGAATTAGCTAAGAAACCCCAGAACTCCTCTGAAGAGGCCCTGCTCGTCGAGGATGAGAATGTCAAGGTCAAAGGGACTGGTTCGGGCTTTGTATGGGATAAATTTGGCCATATCGTAACTAATTACCATGTTGTTTCCGCATTGGCTACTGATAACAGTGGATTGCAGCGTTGTAAGGTAAATTTAGTCGATGCTAAAGGAAATGGAATTTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTCAAGGTGGAACTTGGAGGATGTGAACTAAAGCCCATCGTTCTCGGTACCTCTCGAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTGATCAGTGGATTGGGTAGAGAAATTCCATCACCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTTCAGGGAATTCAGGGGGGCCATTAATTGACTCCTACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATTCCAATAGACACAGTTGTACGTACGGTTCCATACCTTATTGTATACGGAACACCTTACAGTGAGAGATTT

Coding sequence (CDS)

ATGGCGTTAGCCTCACTGGGAATTCATCTTCTTCCAATTCCAGCTCCCCCAAATTCTTCCCACAACTCTCTACCCTTCACTTCGCGAAGAGCCCTAGTTTTTGCCCCATCTGCTTTGATGGCTTCCCTCCTCGCTTTCCCTCTTCCCACTCACGCCGCTCTCCCCCAACTACAGGACCAGGTTCCACAAGAAGAAGATCGAGTCGTCGCTCTCTTTCAGGATGCTTCACCTTCTGTCGTTTACATTAAGGACCTTGAATTAGCTAAGAAACCCCAGAACTCCTCTGAAGAGGCCCTGCTCGTCGAGGATGAGAATGTCAAGGTCAAAGGGACTGGTTCGGGCTTTGTATGGGATAAATTTGGCCATATCGTAACTAATTACCATGTTGTTTCCGCATTGGCTACTGATAACAGTGGATTGCAGCGTTGTAAGGTAAATTTAGTCGATGCTAAAGGAAATGGAATTTATAGGGAAGCAAAAATTGTAGGTTTTGATCCAGAGTATGATCTAGCTGTTCTCAAGGTGGAACTTGGAGGATGTGAACTAAAGCCCATCGTTCTCGGTACCTCTCGAAATTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTGATCAGTGGATTGGGTAGAGAAATTCCATCACCAAATGGAAGGGCCATTCGGGGGGCTATTCAGACAGATGCTGCTATTAGTTCAGGGAATTCAGGGGGGCCATTAATTGACTCCTACGGTCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATTCCAATAGACACAGTTGTACGTACGGTTCCATACCTTATTGTATACGGAACACCTTACAGTGAGAGATTT

Protein sequence

MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQVPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF

Homology

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BLAST of MS013468 vs. NCBI nr
Match: XP_022140016.1 (protease Do-like 5, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 595.9 bits (1535), Expect = 2.0e-166
Identity = 301/304 (99.01%), Postives = 302/304 (99.34%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQ+Q Q
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120
           VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 304

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BLAST of MS013468 vs. NCBI nr
Match: XP_022140015.1 (protease Do-like 5, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 558.9 bits (1439), Expect = 2.7e-155
Identity = 285/293 (97.27%), Postives = 288/293 (98.29%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQ+Q Q
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120
           VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYL 294
           AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT V T P++
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT-VGTQPFM 292

[Close]

BLAST of MS013468 vs. NCBI nr
Match: XP_022927238.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 537.7 bits (1384), Expect = 6.4e-149
Identity = 270/305 (88.52%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL SLGI LLP+ +PPNSS  SLPFTSRRA+VFAP+ALMASLLAFP+P+ AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVE-DENVKVKGTGSGFVWDK 120
           VPQEEDR+V LFQ+ SPSVVYIK+LE+AKKPQN SEEA+L+E DEN KVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVD KGNGI R+AKIVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+D YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

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BLAST of MS013468 vs. NCBI nr
Match: XP_008444456.1 (PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protease Do-like 5 [Cucumis melo var. makuwa] >TYK20678.1 protease Do-like 5 [Cucumis melo var. makuwa])

HSP 1 Score: 535.4 bits (1378), Expect = 3.2e-148
Identity = 271/305 (88.85%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL  LGI  LPIPAPPNSS N LPFTSRRA++F+P+ALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEE-ALLVEDENVKVKGTGSGFVWDK 120
           + QEEDR+V+LFQ+ SPSVVYIKDLELAK PQN SEE  +L+ED+NVKVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVD KGNGIY+EA IVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of MS013468 vs. NCBI nr
Match: XP_038893976.1 (protease Do-like 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 535.0 bits (1377), Expect = 4.1e-148
Identity = 269/304 (88.49%), Postives = 283/304 (93.09%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL SLGI LLPI +PPNS+ N LP TSRRA+VFAP+ALMASLLAFP+PT+AALPQLQD 
Sbjct: 1   MALGSLGIRLLPISSPPNSAENPLPITSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120
           +PQEEDR+VALFQ+ SPSVVYIKDLE+AK PQN S      EDEN KVKGTGSGFVWDKF
Sbjct: 61  IPQEEDRIVALFQETSPSVVYIKDLEVAKNPQNPSG-----EDENAKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVD KGNGIY+EAKIVGFDPEYDLAVLKVEL G 
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180

Query: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIV GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA
Sbjct: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240

Query: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV+RTVPYLIVYGTPY
Sbjct: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVIRTVPYLIVYGTPY 299

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 299

[Close]

BLAST of MS013468 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-99
Identity = 191/295 (64.75%), Postives = 229/295 (77.63%), Query Frame = 0


Query: 19  SSHNSLPFTSRRALVFAPS-ALMASLLA-----FPLPTHAALPQL---QDQVPQEEDRVV 78
           S+H  +    RR ++F  S AL +SLL       P+ +  AL Q    ++++ +EE+R V
Sbjct: 33  SNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNV 92

Query: 79  ALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFGHIVTNYHV 138
            LFQ  SPSVVYI+ +EL K    +S   +L ++EN K++GTGSGFVWDK GHIVTNYHV
Sbjct: 93  NLFQKTSPSVVYIEAIELPK----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHV 152

Query: 139 VSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGCELKPIVLGT 198
           ++ LATD  GLQRCKV+LVDAKG    +E KIVG DP+ DLAVLK+E  G EL P+VLGT
Sbjct: 153 IAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGT 212

Query: 199 SRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISSGNSGG 258
           S +LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I+SGNSGG
Sbjct: 213 SNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGG 272

Query: 259 PLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305
           PL+DSYGH IGVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 273 PLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]

BLAST of MS013468 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-48
Identity = 133/297 (44.78%), Postives = 179/297 (60.27%), Query Frame = 0


Query: 8   IHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQV------ 67
           +H L + + P+++   L  +    L F PS  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFG 127
              E R+V LF+  + SVV I D+ L  +PQ      + + +      G GSG VWD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 187
           +IVTNYHV+ +AL+ + S G    +VN++ + G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI +G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]

BLAST of MS013468 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 3.3e-47
Identity = 121/266 (45.49%), Postives = 164/266 (61.65%), Query Frame = 0


Query: 33  VFAPSALMASLLAFPLPTHAALPQLQDQVPQEEDRVVALFQDASPSVVYIKDLELAKKPQ 92
           +FA S  + S  AF + T         ++  +E   V LFQ+ +PSVVYI +L +     
Sbjct: 93  LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSVVYITNLAV----- 152

Query: 93  NSSEEALLVEDENVKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAKG 152
              ++A  ++   V  +G+GSGFVWDK GHIVTNYHV+        G    +V L D   
Sbjct: 153 --RQDAFTLDVLEVP-QGSGSGFVWDKQGHIVTNYHVI-------RGASDLRVTLADQ-- 212

Query: 153 NGIYREAKIVGFDPEYDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTL 212
                +AK+VGFD + D+AVL+++    +L+PI +G S +L VGQ  +AIGNPFG + TL
Sbjct: 213 --TTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTL 272

Query: 213 TAGVISGLGREIPS-PNGRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGT 272
           T GVISGL REI S   GR I+  IQTDAAI+ GNSGGPL+DS G +IG+NTA ++   +
Sbjct: 273 TTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYS--PS 330

Query: 273 GMSSGVNFAIPIDTVVRTVPYLIVYG 298
           G SSGV F+IP+DTV   V  L+ +G
Sbjct: 333 GASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]

BLAST of MS013468 vs. ExPASy Swiss-Prot
Match: Q2SL36 (Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=mucD PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.1e-30
Identity = 81/181 (44.75%), Postives = 111/181 (61.33%), Query Frame = 0


Query: 107 KVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDP 166
           + + TGSGF+  K G+I+TN HVV       +G     V L+D +       AK++G D 
Sbjct: 87  EAQSTGSGFIVSKDGYILTNNHVV-------AGADEIFVRLMDRR----ELTAKLIGSDE 146

Query: 167 EYDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPS 226
           + DLAVLKVE    +L  + LG S  L+VG+   AIG+PFG+E T+TAG++S  GR +P+
Sbjct: 147 KSDLAVLKVEAD--DLPVLNLGKSSELKVGEWVVAIGSPFGFEYTVTAGIVSAKGRSLPN 206

Query: 227 PNGRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 286
            N       IQTD AI+ GNSGGPL +  G V+G+N+  +TR G  M  GV+FAIPID  
Sbjct: 207 ENYVPF---IQTDVAINPGNSGGPLFNLEGEVVGINSQIYTRSGGFM--GVSFAIPIDVA 249

Query: 287 V 288
           +
Sbjct: 267 L 249

[Close]

BLAST of MS013468 vs. ExPASy Swiss-Prot
Match: Q4KGQ4 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=mucD PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.4e-28
Identity = 73/185 (39.46%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 103 DENVKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIV 162
           D   + +  GSGF+    G+I+TN HV+       +      V L D        +AK++
Sbjct: 91  DRQREAQSLGSGFIISADGYILTNNHVI-------ADADEILVRLADRS----ELKAKLI 150

Query: 163 GFDPEYDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGR 222
           G DP  D+A+LK++  G +L  + LG S++L+ GQ   AIG+PFG++ T+T G++S +GR
Sbjct: 151 GTDPRSDVALLKID--GKDLPVLKLGKSQDLKAGQWVVAIGSPFGFDHTVTQGIVSAIGR 210

Query: 223 EIPSPNGRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIP 282
            +P+ N       IQTD  I+ GNSGGPL +  G V+G+N+  +TR G  M  GV+FAIP
Sbjct: 211 SLPNENYVPF---IQTDVPINPGNSGGPLFNLAGEVVGINSQIYTRSGGFM--GVSFAIP 257

Query: 283 IDTVV 288
           ID  +
Sbjct: 271 IDVAM 257

[Close]

BLAST of MS013468 vs. ExPASy TrEMBL
Match: A0A6J1CEE6 (protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 9.5e-167
Identity = 301/304 (99.01%), Postives = 302/304 (99.34%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQ+Q Q
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120
           VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 304

[Close]

BLAST of MS013468 vs. ExPASy TrEMBL
Match: A0A6J1CDW0 (protease Do-like 5, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.3e-155
Identity = 285/293 (97.27%), Postives = 288/293 (98.29%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQ+Q Q
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120
           VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGC 180

Query: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRG IQTDA
Sbjct: 181 ELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTDA 240

Query: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYL 294
           AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT V T P++
Sbjct: 241 AISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDT-VGTQPFM 292

[Close]

BLAST of MS013468 vs. ExPASy TrEMBL
Match: A0A6J1EKF8 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.1e-149
Identity = 270/305 (88.52%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL SLGI LLP+ +PPNSS  SLPFTSRRA+VFAP+ALMASLLAFP+P+ AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVE-DENVKVKGTGSGFVWDK 120
           VPQEEDR+V LFQ+ SPSVVYIK+LE+AKKPQN SEEA+L+E DEN KVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVD KGNGI R+AKIVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+D YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of MS013468 vs. ExPASy TrEMBL
Match: A0A5D3DAW0 (Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00660 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.5e-148
Identity = 271/305 (88.85%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL  LGI  LPIPAPPNSS N LPFTSRRA++F+P+ALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEE-ALLVEDENVKVKGTGSGFVWDK 120
           + QEEDR+V+LFQ+ SPSVVYIKDLELAK PQN SEE  +L+ED+NVKVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVD KGNGIY+EA IVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of MS013468 vs. ExPASy TrEMBL
Match: A0A1S3B9W5 (protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.5e-148
Identity = 271/305 (88.85%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQ 60
           MAL  LGI  LPIPAPPNSS N LPFTSRRA++F+P+ALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEE-ALLVEDENVKVKGTGSGFVWDK 120
           + QEEDR+V+LFQ+ SPSVVYIKDLELAK PQN SEE  +L+ED+NVKVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVD KGNGIY+EA IVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+DSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of MS013468 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 364.4 bits (934), Expect = 9.0e-101
Identity = 191/295 (64.75%), Postives = 229/295 (77.63%), Query Frame = 0


Query: 19  SSHNSLPFTSRRALVFAPS-ALMASLLA-----FPLPTHAALPQL---QDQVPQEEDRVV 78
           S+H  +    RR ++F  S AL +SLL       P+ +  AL Q    ++++ +EE+R V
Sbjct: 33  SNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNV 92

Query: 79  ALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFGHIVTNYHV 138
            LFQ  SPSVVYI+ +EL K    +S   +L ++EN K++GTGSGFVWDK GHIVTNYHV
Sbjct: 93  NLFQKTSPSVVYIEAIELPK----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHV 152

Query: 139 VSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGGCELKPIVLGT 198
           ++ LATD  GLQRCKV+LVDAKG    +E KIVG DP+ DLAVLK+E  G EL P+VLGT
Sbjct: 153 IAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGT 212

Query: 199 SRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISSGNSGG 258
           S +LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I+SGNSGG
Sbjct: 213 SNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGG 272

Query: 259 PLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305
           PL+DSYGH IGVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 273 PLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]

BLAST of MS013468 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 195.3 bits (495), Expect = 7.2e-50
Identity = 133/297 (44.78%), Postives = 179/297 (60.27%), Query Frame = 0


Query: 8   IHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQV------ 67
           +H L + + P+++   L  +    L F PS  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFG 127
              E R+V LF+  + SVV I D+ L  +PQ      + + +      G GSG VWD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 187
           +IVTNYHV+ +AL+ + S G    +VN++ + G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI +G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]

BLAST of MS013468 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 190.3 bits (482), Expect = 2.3e-48
Identity = 121/266 (45.49%), Postives = 164/266 (61.65%), Query Frame = 0


Query: 33  VFAPSALMASLLAFPLPTHAALPQLQDQVPQEEDRVVALFQDASPSVVYIKDLELAKKPQ 92
           +FA S  + S  AF + T         ++  +E   V LFQ+ +PSVVYI +L +     
Sbjct: 93  LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSVVYITNLAV----- 152

Query: 93  NSSEEALLVEDENVKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAKG 152
              ++A  ++   V  +G+GSGFVWDK GHIVTNYHV+        G    +V L D   
Sbjct: 153 --RQDAFTLDVLEVP-QGSGSGFVWDKQGHIVTNYHVI-------RGASDLRVTLADQ-- 212

Query: 153 NGIYREAKIVGFDPEYDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTL 212
                +AK+VGFD + D+AVL+++    +L+PI +G S +L VGQ  +AIGNPFG + TL
Sbjct: 213 --TTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTL 272

Query: 213 TAGVISGLGREIPS-PNGRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGT 272
           T GVISGL REI S   GR I+  IQTDAAI+ GNSGGPL+DS G +IG+NTA ++   +
Sbjct: 273 TTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYS--PS 330

Query: 273 GMSSGVNFAIPIDTVVRTVPYLIVYG 298
           G SSGV F+IP+DTV   V  L+ +G
Sbjct: 333 GASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]

BLAST of MS013468 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 172.9 bits (437), Expect = 3.8e-43
Identity = 124/297 (41.75%), Postives = 169/297 (56.90%), Query Frame = 0


Query: 8   IHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQLQDQV------ 67
           +H L + + P+++   L  +    L F PS  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKFG 127
              E R+V LF+  + SVV I D+ L  +PQ      + + +      G GSG VWD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 187
           +IVTNYHV+ +AL+ + S G    +VN++ + G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI +G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+                TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ----------------TVLKIVPQLIQF 325

[Close]

BLAST of MS013468 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 86.7 bits (213), Expect = 3.6e-17
Identity = 62/186 (33.33%), Postives = 95/186 (51.08%), Query Frame = 0


Query: 109 KGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDAK-GNGIYREAKIVGFDPE 168
           K  GSG + D  G I+T  HVV     D   ++      VD    +G   E  +V  D +
Sbjct: 146 KSIGSGTIIDADGTILTCAHVV----VDFQNIRHSSKGRVDVTLQDGRTFEGVVVNADLQ 205

Query: 169 YDLAVLKVELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSP 228
            D+A++K++     L    LG S  LR G    A+G P   + T+TAG++S + R+    
Sbjct: 206 SDIALVKIK-SKTPLPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDRKSSDL 265

Query: 229 N-GRAIRGAIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 288
             G   R  +QTD +I++GNSGGPL++  G VIGVN           + G+ F++PID+V
Sbjct: 266 GLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKVL-----AADGLGFSVPIDSV 321

Query: 289 VRTVPY 293
            + + +
Sbjct: 326 SKIIEH 321

The following BLAST results are available for this feature:

BLAST of MS013468 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140016.1	2.0e-166	99.01	protease Do-like 5, chloroplastic isoform X2 [Momordica charantia]	[more]
XP_022140015.1	2.7e-155	97.27	protease Do-like 5, chloroplastic isoform X1 [Momordica charantia]	[more]
XP_022927238.1	6.4e-149	88.52	protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata]	[more]
XP_008444456.1	3.2e-148	88.85	PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protea...	[more]
XP_038893976.1	4.1e-148	88.49	protease Do-like 5, chloroplastic [Benincasa hispida]	[more]

BLAST of MS013468 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEL7	1.3e-99	64.75	Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ...	[more]
Q9LU10	1.0e-48	44.78	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
O22609	3.3e-47	45.49	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q2SL36	2.1e-30	44.75	Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain...	[more]
Q4KGQ4	4.4e-28	39.46	Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (s...	[more]

BLAST of MS013468 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEE6	9.5e-167	99.01	protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1CDW0	1.3e-155	97.27	protease Do-like 5, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1EKF8	3.1e-149	88.52	protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A5D3DAW0	1.5e-148	88.85	Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G0...	[more]
A0A1S3B9W5	1.5e-148	88.85	protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 S...	[more]

BLAST of MS013468 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18370.1	9.0e-101	64.75	DEGP protease 5	[more]
AT5G39830.1	7.2e-50	44.78	Trypsin family protein with PDZ domain	[more]
AT3G27925.1	2.3e-48	45.49	DegP protease 1	[more]
AT5G39830.2	3.8e-43	41.75	Trypsin family protein with PDZ domain	[more]
AT5G27660.1	3.6e-17	33.33	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 195..219 score: 48.88 coord: 254..271 score: 37.67 coord: 121..133 score: 50.47 coord: 232..249 score: 62.74
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 60..185 e-value: 1.2E-30 score: 107.7
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 186..298 e-value: 3.1E-40 score: 138.9
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 112..261 e-value: 4.7E-34 score: 118.5
None	No IPR available	PANTHER	PTHR43343	PEPTIDASE S12	coord: 18..304
None	No IPR available	PANTHER	PTHR43343:SF6	PROTEASE DO-LIKE 5, CHLOROPLASTIC ISOFORM X1	coord: 18..304
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 70..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS013468.1	MS013468.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity