MS013458 (gene) Bitter gourd (TR) v1

Overview


Name	MS013458
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Proton pump-interactor 1-like
Location	scaffold402: 1688716 .. 1689323 (-)
RNA-Seq Expression	MS013458
Synteny	MS013458

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTACATAGAAGCCAAAATTTGGCCAATGAGAAGAGGCTATTAAGGGAGATCAAGAGGGAAGCTGGTGGAACAACACTTAATGAACTTGATGCTCCTGTACTTAAATCCATCCTTAATTTTTCTTTTTCTTGTGGGTTTCTGGATATATATGAATTAATTAATTTTCCTATTTGATTTTCTCGGAAACCAAACAGAACGAGAAGTTGAATGAGCAGATGAAGAACTCCCGTTTGTATTTATACAGAACAGATTTTGAAGAGATTCACAAAGAAATCAGAAGGCAAGGCTGTGGCCAATGCTGCAGTGGGGGGAGCCCTCTGGAGCTCTTTGGGATCAAAAAAACATATTCAACTACAAGTTAAAGTATAAAGAAATTAAATGGGAAAAATAATTAACTTTTTATTTTGTGAAAGAAAAAAAGAGAAGAAATCTTTGATTTAACAGCTAAATTATGATATTAAGTGCAGGTTATGAACGAGGAATTAGATGAGCTGAGGTCGAAGCAAGTTGAAGCTCGGAACGAAGTCAAGCTTCTCGAGGAAAAATTGAGGATTTTAGAAAAGCAAATAATGTTTTTGCATAAACAATTAAAGACTCTAATCATA

mRNA sequence

ATGCTACATAGAAGCCAAAATTTGGCCAATGAGAAGAGGCTATTAAGGGAGATCAAGAGGGAAGCTGGTGGAACAACACTTAATGAACTTGATGCTCCTAACGAGAAGTTGAATGAGCAGATGAAGAACTCCCGTTTGTATTTATACAGAACAGATTTTGAAGAGATTCACAAAGAAATCAGAAGGAAGGCTGTGGCCAATGCTGCAGTGGGGGGAGCCCTCTGGAGCTCTTTGGGATCAAAAAAACATATTCAACTACAAGTTAAAGTTATGAACGAGGAATTAGATGAGCTGAGGTCGAAGCAAGTTGAAGCTCGGAACGAAGTCAAGCTTCTCGAGGAAAAATTGAGGATTTTAGAAAAGCAAATAATGTTTTTGCATAAACAATTAAAGACTCTAATCATA

Coding sequence (CDS)

ATGCTACATAGAAGCCAAAATTTGGCCAATGAGAAGAGGCTATTAAGGGAGATCAAGAGGGAAGCTGGTGGAACAACACTTAATGAACTTGATGCTCCTAACGAGAAGTTGAATGAGCAGATGAAGAACTCCCGTTTGTATTTATACAGAACAGATTTTGAAGAGATTCACAAAGAAATCAGAAGGAAGGCTGTGGCCAATGCTGCAGTGGGGGGAGCCCTCTGGAGCTCTTTGGGATCAAAAAAACATATTCAACTACAAGTTAAAGTTATGAACGAGGAATTAGATGAGCTGAGGTCGAAGCAAGTTGAAGCTCGGAACGAAGTCAAGCTTCTCGAGGAAAAATTGAGGATTTTAGAAAAGCAAATAATGTTTTTGCATAAACAATTAAAGACTCTAATCATA

Protein sequence

MLHRSQNLANEKRLLREIKREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEIHKEIRRKAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVKLLEEKLRILEKQIMFLHKQLKTLII

Homology

[Close]

BLAST of MS013458 vs. NCBI nr
Match: KAA0054011.1 (proton pump-interactor 1-like [Cucumis melo var. makuwa] >TYK20697.1 proton pump-interactor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 151.8 bits (382), Expect = 4.4e-33
Identity = 87/141 (61.70%), Postives = 109/141 (77.30%), Query Frame = 0


Query: 1   MLHRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEI 60
           M H S+N+ANEK+LLREI    K++   TT+ ELD P +K N++++NS LYLY TDFE I
Sbjct: 1   MQHGSKNIANEKKLLREINSSKKKKIRTTTIEELDVPIKKKNDEIENSHLYLYTTDFEGI 60

Query: 61  HKEIRR------KAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVK 120
           ++E++R      KAVANAAVGG LWSSLG+KK IQ QV VM EELDELR KQ+E RNE K
Sbjct: 61  NEEMKRLEMVKQKAVANAAVGGKLWSSLGTKKDIQQQVNVMYEELDELRLKQIETRNETK 120

Query: 121 LLEEKLRILEKQIMFLHKQLK 132
             E+KLRI++KQI+FLHKQL+
Sbjct: 121 YGEKKLRIVQKQILFLHKQLE 141

[Close]

BLAST of MS013458 vs. NCBI nr
Match: XP_022955118.1 (uncharacterized protein LOC111457179 isoform X2 [Cucurbita moschata])

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-29
Identity = 79/141 (56.03%), Postives = 108/141 (76.60%), Query Frame = 0


Query: 3   HRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEIHK 62
           H S+N+A EK+LLR+I    K +  GTTL+EL+AP + L+EQ+KNSR++LYRTDFEEI++
Sbjct: 89  HGSKNMAKEKKLLRDINTSQKSKQAGTTLDELEAPIKNLDEQIKNSRMHLYRTDFEEIYR 148

Query: 63  E------IRRKAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVKLL 122
           E       +  AVANA VGG LWSSLGSKKH+Q QV+++ EELDELR KQVE R+  + +
Sbjct: 149 EKKVHEMEKETAVANAGVGGRLWSSLGSKKHVQRQVQILYEELDELRLKQVEIRDGGRFV 208

Query: 123 EEKLRILEKQIMFLHKQLKTL 134
           E+KL  +EKQI+FL  Q++++
Sbjct: 209 EKKLGWIEKQIVFLRTQIESI 229

[Close]

BLAST of MS013458 vs. NCBI nr
Match: XP_022955117.1 (uncharacterized protein LOC111457179 isoform X1 [Cucurbita moschata])

HSP 1 Score: 125.9 bits (315), Expect = 2.6e-25
Identity = 80/171 (46.78%), Postives = 108/171 (63.16%), Query Frame = 0


Query: 3   HRSQNLANEKRLLREI----KREAGGTTLNELDAPNEK---------------------- 62
           H S+N+A EK+LLR+I    K +  GTTL+EL+AP  K                      
Sbjct: 89  HGSKNMAKEKKLLRDINTSQKSKQAGTTLDELEAPVHKLKLLPFKFLCWIISINIFLLIF 148

Query: 63  --------LNEQMKNSRLYLYRTDFEEIHKE------IRRKAVANAAVGGALWSSLGSKK 122
                   L+EQ+KNSR++LYRTDFEEI++E       +  AVANA VGG LWSSLGSKK
Sbjct: 149 SESKQIKNLDEQIKNSRMHLYRTDFEEIYREKKVHEMEKETAVANAGVGGRLWSSLGSKK 208

Query: 123 HIQLQVKVMNEELDELRSKQVEARNEVKLLEEKLRILEKQIMFLHKQLKTL 134
           H+Q QV+++ EELDELR KQVE R+  + +E+KL  +EKQI+FL  Q++++
Sbjct: 209 HVQRQVQILYEELDELRLKQVEIRDGGRFVEKKLGWIEKQIVFLRTQIESI 259

[Close]

BLAST of MS013458 vs. NCBI nr
Match: KAG6573375.1 (hypothetical protein SDJN03_27262, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 108.6 bits (270), Expect = 4.2e-20
Identity = 69/145 (47.59%), Postives = 87/145 (60.00%), Query Frame = 0


Query: 3   HRSQNLANEKRLLREI----KREAGGTTLNELDAPNEK---------------------- 62
           H S+N+A EK+LLR+I    K +  GTTL+EL+AP  K                      
Sbjct: 12  HGSKNMAKEKKLLRDINTSQKSKQAGTTLDELEAPVHKLKLLPFKFLCWIISINIFLLIF 71

Query: 63  --------LNEQMKNSRLYLYRTDFEEI------HKEIRRKAVANAAVGGALWSSLGSKK 108
                   L+EQ+KNS ++LYRTDFEEI      H+  +  AVANA VGG LWSSLGSKK
Sbjct: 72  SESKQIKNLDEQIKNSHMHLYRTDFEEICREKKVHEMEKETAVANAGVGGRLWSSLGSKK 131

[Close]

BLAST of MS013458 vs. NCBI nr
Match: XP_016899262.1 (PREDICTED: uncharacterized protein LOC103489454 [Cucumis melo])

HSP 1 Score: 104.8 bits (260), Expect = 6.1e-19
Identity = 59/102 (57.84%), Postives = 75/102 (73.53%), Query Frame = 0


Query: 1   MLHRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEI 60
           M H S+N+ANEK+LLREI    K++   TT+ ELD P +K N++++NS LYLY TDFE I
Sbjct: 93  MQHGSKNIANEKKLLREINSSKKKKIRTTTIEELDVPIKKKNDEIENSHLYLYTTDFEGI 152

Query: 61  HKEIRR------KAVANAAVGGALWSSLGSKKHIQLQVKVMN 93
           ++E++R      KAVANAAVGG LWSSLG+KK IQ QV V N
Sbjct: 153 NEEMKRLEMVKQKAVANAAVGGKLWSSLGTKKDIQQQVNVKN 194

[Close]

BLAST of MS013458 vs. ExPASy TrEMBL
Match: A0A0A0LVC7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G534760 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.2e-33
Identity = 87/143 (60.84%), Postives = 110/143 (76.92%), Query Frame = 0


Query: 1   MLHRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEI 60
           M H S+N+A+EK+LLREI    K+E   TT+ ELD P +K NE+++NS LYLY TDFE I
Sbjct: 1   MQHGSKNIASEKKLLREISSSRKKEIRSTTVEELDVPIKKKNEEIENSHLYLYTTDFEGI 60

Query: 61  HKEIRR------KAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVK 120
           +KE++R      KAVANAAVGG LWSSLG+KK IQ +V VM EELDELR KQ+E RNE K
Sbjct: 61  NKEMKRLEMVKEKAVANAAVGGKLWSSLGTKKDIQQKVNVMYEELDELRLKQIETRNETK 120

Query: 121 LLEEKLRILEKQIMFLHKQLKTL 134
             E+KLRI++KQI++LHKQL+ +
Sbjct: 121 YGEKKLRIIQKQILYLHKQLEII 143

[Close]

BLAST of MS013458 vs. ExPASy TrEMBL
Match: A0A5D3DAS6 (Proton pump-interactor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00910 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.1e-33
Identity = 87/141 (61.70%), Postives = 109/141 (77.30%), Query Frame = 0


Query: 1   MLHRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEI 60
           M H S+N+ANEK+LLREI    K++   TT+ ELD P +K N++++NS LYLY TDFE I
Sbjct: 1   MQHGSKNIANEKKLLREINSSKKKKIRTTTIEELDVPIKKKNDEIENSHLYLYTTDFEGI 60

Query: 61  HKEIRR------KAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVK 120
           ++E++R      KAVANAAVGG LWSSLG+KK IQ QV VM EELDELR KQ+E RNE K
Sbjct: 61  NEEMKRLEMVKQKAVANAAVGGKLWSSLGTKKDIQQQVNVMYEELDELRLKQIETRNETK 120

Query: 121 LLEEKLRILEKQIMFLHKQLK 132
             E+KLRI++KQI+FLHKQL+
Sbjct: 121 YGEKKLRIVQKQILFLHKQLE 141

[Close]

BLAST of MS013458 vs. ExPASy TrEMBL
Match: A0A6J1GT27 (uncharacterized protein LOC111457179 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457179 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 6.4e-30
Identity = 79/141 (56.03%), Postives = 108/141 (76.60%), Query Frame = 0


Query: 3   HRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEIHK 62
           H S+N+A EK+LLR+I    K +  GTTL+EL+AP + L+EQ+KNSR++LYRTDFEEI++
Sbjct: 89  HGSKNMAKEKKLLRDINTSQKSKQAGTTLDELEAPIKNLDEQIKNSRMHLYRTDFEEIYR 148

Query: 63  E------IRRKAVANAAVGGALWSSLGSKKHIQLQVKVMNEELDELRSKQVEARNEVKLL 122
           E       +  AVANA VGG LWSSLGSKKH+Q QV+++ EELDELR KQVE R+  + +
Sbjct: 149 EKKVHEMEKETAVANAGVGGRLWSSLGSKKHVQRQVQILYEELDELRLKQVEIRDGGRFV 208

Query: 123 EEKLRILEKQIMFLHKQLKTL 134
           E+KL  +EKQI+FL  Q++++
Sbjct: 209 EKKLGWIEKQIVFLRTQIESI 229

[Close]

BLAST of MS013458 vs. ExPASy TrEMBL
Match: A0A6J1GSQ0 (uncharacterized protein LOC111457179 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457179 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.2e-25
Identity = 80/171 (46.78%), Postives = 108/171 (63.16%), Query Frame = 0


Query: 3   HRSQNLANEKRLLREI----KREAGGTTLNELDAPNEK---------------------- 62
           H S+N+A EK+LLR+I    K +  GTTL+EL+AP  K                      
Sbjct: 89  HGSKNMAKEKKLLRDINTSQKSKQAGTTLDELEAPVHKLKLLPFKFLCWIISINIFLLIF 148

Query: 63  --------LNEQMKNSRLYLYRTDFEEIHKE------IRRKAVANAAVGGALWSSLGSKK 122
                   L+EQ+KNSR++LYRTDFEEI++E       +  AVANA VGG LWSSLGSKK
Sbjct: 149 SESKQIKNLDEQIKNSRMHLYRTDFEEIYREKKVHEMEKETAVANAGVGGRLWSSLGSKK 208

Query: 123 HIQLQVKVMNEELDELRSKQVEARNEVKLLEEKLRILEKQIMFLHKQLKTL 134
           H+Q QV+++ EELDELR KQVE R+  + +E+KL  +EKQI+FL  Q++++
Sbjct: 209 HVQRQVQILYEELDELRLKQVEIRDGGRFVEKKLGWIEKQIVFLRTQIESI 259

[Close]

BLAST of MS013458 vs. ExPASy TrEMBL
Match: A0A1S4DTD6 (uncharacterized protein LOC103489454 OS=Cucumis melo OX=3656 GN=LOC103489454 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.0e-19
Identity = 59/102 (57.84%), Postives = 75/102 (73.53%), Query Frame = 0


Query: 1   MLHRSQNLANEKRLLREI----KREAGGTTLNELDAPNEKLNEQMKNSRLYLYRTDFEEI 60
           M H S+N+ANEK+LLREI    K++   TT+ ELD P +K N++++NS LYLY TDFE I
Sbjct: 93  MQHGSKNIANEKKLLREINSSKKKKIRTTTIEELDVPIKKKNDEIENSHLYLYTTDFEGI 152

Query: 61  HKEIRR------KAVANAAVGGALWSSLGSKKHIQLQVKVMN 93
           ++E++R      KAVANAAVGG LWSSLG+KK IQ QV V N
Sbjct: 153 NEEMKRLEMVKQKAVANAAVGGKLWSSLGTKKDIQQQVNVKN 194

[Close]

BLAST of MS013458 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 44.3 bits (103), Expect = 9.1e-05
Identity = 36/112 (32.14%), Postives = 58/112 (51.79%), Query Frame = 0


Query: 38  NEQMKNSRLYLYR----------TDFEEIHKEI------RRKAVANAAVGGALWSSLGSK 97
           +E+  NS +Y Y+          T+ ++I KEI      R K +ANAA+   +  S+G K
Sbjct: 178 SEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQK 237

Query: 98  KHIQLQVKVMNEELDELRSKQVEARNEVKLLEEKLRILEKQIMFLHKQLKTL 134
             IQ QVK+M   LD ++ ++      +  L EKL+  + +I  L  +LKT+
Sbjct: 238 DDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTV 289

The following BLAST results are available for this feature:

BLAST of MS013458 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0054011.1	4.4e-33	61.70	proton pump-interactor 1-like [Cucumis melo var. makuwa] >TYK20697.1 proton pump...	[more]
XP_022955118.1	1.3e-29	56.03	uncharacterized protein LOC111457179 isoform X2 [Cucurbita moschata]	[more]
XP_022955117.1	2.6e-25	46.78	uncharacterized protein LOC111457179 isoform X1 [Cucurbita moschata]	[more]
KAG6573375.1	4.2e-20	47.59	hypothetical protein SDJN03_27262, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_016899262.1	6.1e-19	57.84	PREDICTED: uncharacterized protein LOC103489454 [Cucumis melo]	[more]

BLAST of MS013458 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS013458 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVC7	1.2e-33	60.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G534760 PE=4 SV=1	[more]
A0A5D3DAS6	2.1e-33	61.70	Proton pump-interactor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A6J1GT27	6.4e-30	56.03	uncharacterized protein LOC111457179 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GSQ0	1.2e-25	46.78	uncharacterized protein LOC111457179 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A1S4DTD6	3.0e-19	57.84	uncharacterized protein LOC103489454 OS=Cucumis melo OX=3656 GN=LOC103489454 PE=...	[more]

BLAST of MS013458 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27500.1	9.1e-05	32.14	proton pump interactor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 81..135
None	No IPR available	PANTHER	PTHR32219	RNA-BINDING PROTEIN YLMH-RELATED	coord: 1..133
IPR029669	Proton pump-interactor	PANTHER	PTHR32219:SF2	PROTON PUMP-INTERACTOR 1	coord: 1..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS013458.1	MS013458.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010155	regulation of proton transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005886	plasma membrane