MS013435 (gene) Bitter gourd (TR) v1

Overview
NameMS013435
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWAT1-related protein
Locationscaffold402: 1515846 .. 1517665 (+)
RNA-Seq ExpressionMS013435
SyntenyMS013435
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCAAAAAGGTGCAAAAATGAAGCCTGTTGTAGTAATGCTTTCGATTAACCTTGCGCTCGCTGGTGTAAATATACTACTTAAGGAGATTCTTAGTGAAGGGATTAGTCAGCTAGTTATCGTTATGTACCGGCAAACAATTTCCGCTCTATTCTTGTCGCCAATTGCCTATTTTTGGGAGAGGTAAGGGCACCTTGACTTGTCCAGAAAATATCAGTTCCTTTACCATGGAGTCTGCATTTTCCTTATTATCCTTGTTAAGTTTGCTCTGTTGTGTGAAAAGATTAAATGCTATAGAACTATAGAGTTGTCTTTTTTTGATGAAATGCAGGAAAACCAGACCTCAGCTTACGGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTAGTTGGGTTAGTAATAGCATCTTTTGTAAACTTTACCATAACTTTATGATGTTATTGTCACTATGAAACTTTGGAAAGATGTACAAATGCAGGCTAACACTAACATTATATCTATTCCTAATTGGTCTGCAATACACATCTGCCACTTTCTCTTGTGCATTTCTCAACCTGGTGCCCGTAAATACGTTCATTCTAGCCGTATTGTTCGGGTGCGTGTCAAAAAATTGTAGTGCATTTTCTTTCTAAATATTAACTCAAATGTGATGATAGTAACTCTGGTTTCAAAATATTTCTAGTATGGAGAAAGTGGACATGAAAAGCAAGGGTGGAAGAGCCAAAGTACTTGGGACACTGGTTTGCATTTCAGGAAACTTAATTTTGATTCTGTACAAAGGAATGCCGTTAACCAGCCCGGGATCAGCAACCGGGAAAACAAACGGGGTTCATCCGACAGTTCAACCTGAAAGGCCAGAGAGATGGTTGATAGGTTCATTGGTTTTGACTGCAGGCTGCTTCATGTGGTCCTCTTGGTTCCTAATGCAATCAAGGGTAGGCAAGGTTTATCCATGCCAATATTCTAGTACCTGCATCATGTCCTTTTTCAGCGCCATCCAGTCGGTGGTTTTACATTTGATTATCGACACAAAGAACTCTGTGTTCGTCGTGAAAGGGAAGTCTGCTATCTTAAGCCTCATATATGCTGTAAGTATCAGTTTGTTTTTTCTAACTTCAGCAGCTATTTGTATCTAAAATTCTTTGCAATTTGATGATATTGTGATGGGTTTAACAGGGATCAGTGGGTTCAGGGTTGTGCTATGTGGGGATGTCATGGTGTGTGAAAAAGAAAGGGCCAGTTTTCACTGCTGCTTTTACCCCTTTCATGGAGTTGTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAGCAGATTCACCTTGGAAGGTCCACTATTTCTCTTTCTTTTTGCTTTCCTTTTTTGTTCACAAAAGACCAACCACCTTGAAAGATCAAATTCCTTGGTTCTGTCTGCTATTTTTCAAAAGCACTTTCAAGATCTTAAACCAAATCTCAAAACCAAAAGTGGGTATTTGAAAACTACTTATTTTAGTTTCTAAAACACCCCAAAATCATCATCAAACAAGCTTGGAAAATTGCTGATTTCATATCTAGAGACAGAATCAATGTTGTAACAACTAATTAACAACATACCACTTCAAATTCATCTAGATCAAAACTATATGAACTATATGAACTAATTAACAACATACCACTCAACTAATTAACAACATACCACTTCAAGTTTTGGCAGAGACTATATGAACTCATGTCAAAAAGTTTTTGGTGGCAGTGTTGTGGGGTCAGTGTTGATCATAAGTGGAATGTATATTCTTCTGTGGGGTAAAGATAGAGAAGCAAAGGAATGTGCTGTGAAG

mRNA sequence

AAGCAAAAAGGTGCAAAAATGAAGCCTGTTGTAGTAATGCTTTCGATTAACCTTGCGCTCGCTGGTGTAAATATACTACTTAAGGAGATTCTTAGTGAAGGGATTAGTCAGCTAGTTATCGTTATGTACCGGCAAACAATTTCCGCTCTATTCTTGTCGCCAATTGCCTATTTTTGGGAGAGGAAAACCAGACCTCAGCTTACGGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTAGTTGGGCTAACACTAACATTATATCTATTCCTAATTGGTCTGCAATACACATCTGCCACTTTCTCTTGTGCATTTCTCAACCTGGTGCCCGTAAATACGTTCATTCTAGCCGTATTGTTCGGTATGGAGAAAGTGGACATGAAAAGCAAGGGTGGAAGAGCCAAAGTACTTGGGACACTGGTTTGCATTTCAGGAAACTTAATTTTGATTCTGTACAAAGGAATGCCGTTAACCAGCCCGGGATCAGCAACCGGGAAAACAAACGGGGTTCATCCGACAGTTCAACCTGAAAGGCCAGAGAGATGGTTGATAGGTTCATTGGTTTTGACTGCAGGCTGCTTCATGTGGTCCTCTTGGTTCCTAATGCAATCAAGGGTAGGCAAGGTTTATCCATGCCAATATTCTAGTACCTGCATCATGTCCTTTTTCAGCGCCATCCAGTCGGTGGTTTTACATTTGATTATCGACACAAAGAACTCTGTGTTCGTCGTGAAAGGGAAGTCTGCTATCTTAAGCCTCATATATGCTGGATCAGTGGGTTCAGGGTTGTGCTATGTGGGGATGTCATGGTGTGTGAAAAAGAAAGGGCCAGTTTTCACTGCTGCTTTTACCCCTTTCATGGAGTTGTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAGCAGATTCACCTTGGAAGTGTTGTGGGGTCAGTGTTGATCATAAGTGGAATGTATATTCTTCTGTGGGGTAAAGATAGAGAAGCAAAGGAATGTGCTGTGAAG

Coding sequence (CDS)

AAGCAAAAAGGTGCAAAAATGAAGCCTGTTGTAGTAATGCTTTCGATTAACCTTGCGCTCGCTGGTGTAAATATACTACTTAAGGAGATTCTTAGTGAAGGGATTAGTCAGCTAGTTATCGTTATGTACCGGCAAACAATTTCCGCTCTATTCTTGTCGCCAATTGCCTATTTTTGGGAGAGGAAAACCAGACCTCAGCTTACGGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTAGTTGGGCTAACACTAACATTATATCTATTCCTAATTGGTCTGCAATACACATCTGCCACTTTCTCTTGTGCATTTCTCAACCTGGTGCCCGTAAATACGTTCATTCTAGCCGTATTGTTCGGTATGGAGAAAGTGGACATGAAAAGCAAGGGTGGAAGAGCCAAAGTACTTGGGACACTGGTTTGCATTTCAGGAAACTTAATTTTGATTCTGTACAAAGGAATGCCGTTAACCAGCCCGGGATCAGCAACCGGGAAAACAAACGGGGTTCATCCGACAGTTCAACCTGAAAGGCCAGAGAGATGGTTGATAGGTTCATTGGTTTTGACTGCAGGCTGCTTCATGTGGTCCTCTTGGTTCCTAATGCAATCAAGGGTAGGCAAGGTTTATCCATGCCAATATTCTAGTACCTGCATCATGTCCTTTTTCAGCGCCATCCAGTCGGTGGTTTTACATTTGATTATCGACACAAAGAACTCTGTGTTCGTCGTGAAAGGGAAGTCTGCTATCTTAAGCCTCATATATGCTGGATCAGTGGGTTCAGGGTTGTGCTATGTGGGGATGTCATGGTGTGTGAAAAAGAAAGGGCCAGTTTTCACTGCTGCTTTTACCCCTTTCATGGAGTTGTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAGCAGATTCACCTTGGAAGTGTTGTGGGGTCAGTGTTGATCATAAGTGGAATGTATATTCTTCTGTGGGGTAAAGATAGAGAAGCAAAGGAATGTGCTGTGAAG

Protein sequence

KQKGAKMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVDMKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLGSVVGSVLIISGMYILLWGKDREAKECAVK
Homology
BLAST of MS013435 vs. NCBI nr
Match: XP_022140124.1 (WAT1-related protein At3g30340-like isoform X1 [Momordica charantia])

HSP 1 Score: 632.9 bits (1631), Expect = 1.6e-177
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD
Sbjct: 61  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
           KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG
Sbjct: 241 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SVVGSVLIISGMYILLWGKDREAKECAVK
Sbjct: 301 SVVGSVLIISGMYILLWGKDREAKECAVK 329

BLAST of MS013435 vs. NCBI nr
Match: XP_023520073.1 (WAT1-related protein At3g30340-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.9 bits (1491), Expect = 2.7e-161
Identity = 299/329 (90.88%), Postives = 313/329 (95.14%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSL+GLTLTLYLFLIGL YTSATFSCAFLNLV VNTF+LAVL  MEKVD
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVHVNTFVLAVLLRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNL+LILYKGMPLTSPGSAT KTNG   TVQ E P +WLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKTNGARVTVQAETPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
             AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 TFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SV+GSV+IISGMYILLWGKDREAKECAVK
Sbjct: 301 SVLGSVMIISGMYILLWGKDREAKECAVK 329

BLAST of MS013435 vs. NCBI nr
Match: XP_022927179.1 (WAT1-related protein At3g30340-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 577.8 bits (1488), Expect = 6.1e-161
Identity = 298/328 (90.85%), Postives = 312/328 (95.12%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSL+GLTLTLYLFLIGL YTSATFSCAFLNLVPVNTF+LAVL  MEKVD
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNLILILYKGM LTSPGSAT KTNG   TVQ + P +WLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
             AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 TFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAV 335
           SV+GSV+IISGMYILLWGKDREAKECAV
Sbjct: 301 SVIGSVMIISGMYILLWGKDREAKECAV 328

BLAST of MS013435 vs. NCBI nr
Match: XP_023001437.1 (WAT1-related protein At3g30340-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 577.0 bits (1486), Expect = 1.0e-160
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLS+NLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSVNLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSL+GLTLTLYLFLIGL YTSATFSCAFLNLVPVNTF+LAVL  MEKVD
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNL+LILYKGMPLTSPGSAT K NG   TVQ E P +WLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKINGARVTVQAETPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRV KVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVCKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
             AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 TFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SV+GSV+IISGMYILLWGKDREAKECAVK
Sbjct: 301 SVIGSVMIISGMYILLWGKDREAKECAVK 329

BLAST of MS013435 vs. NCBI nr
Match: XP_038895213.1 (WAT1-related protein At3g30340-like [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.5e-159
Identity = 295/329 (89.67%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LT YILFLLFISSL+GLTLTLYLFLIGL  TSATFSCAFLNLVPVNTFILAVLF MEKVD
Sbjct: 61  LTVYILFLLFISSLLGLTLTLYLFLIGLHITSATFSCAFLNLVPVNTFILAVLFRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MK +GGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGK NG   TV+ E P +WLIGS
Sbjct: 121 MKRRGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKLNGAGATVEAETPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVG+V PCQYSSTCIMSFFSAIQS VLHLIID KNS+F+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGEVCPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSLFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
           K AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 KFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SVVGSVLIISGMYILLWGKDR+AKEC +K
Sbjct: 301 SVVGSVLIISGMYILLWGKDRDAKECILK 329

BLAST of MS013435 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 161/328 (49.09%), Postives = 232/328 (70.73%), Query Frame = 0

Query: 8   KPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQL 67
           K V++M  IN+ L+ VN++ K+++ EG++++V   YR  +  LFL P A F ER  RP+L
Sbjct: 10  KAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKL 69

Query: 68  TAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVDM 127
           T  IL  LF S+L+G +L  Y FLIGL+YTS+TFS AF N+VP  TF LA++F  E +++
Sbjct: 70  TGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNI 129

Query: 128 KSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGSL 187
           KS  GRAK+LGT++CI G L+L LYKG  L+   S   +T+           ++W +GS+
Sbjct: 130 KSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETH-TRTDSTGAMTQKWAMGSI 189

Query: 188 VLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKGK 247
           +L     +WSSWF++Q+++ +VYPCQY+ST I+SFF  IQS +L LI +   S++VVK K
Sbjct: 190 MLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDK 249

Query: 248 SAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLGS 307
             +L+L+Y+G VGSGLCYVGMSWC++++G VFT++F P +++FAAIF F  LHEQI+ GS
Sbjct: 250 FQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGS 309

Query: 308 VVGSVLIISGMYILLWGKDREAKECAVK 336
           V+GS++II G+YILLWGK ++      K
Sbjct: 310 VIGSMVIIVGLYILLWGKSKDKSASVTK 336

BLAST of MS013435 vs. ExPASy Swiss-Prot
Match: Q9M130 (WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.7e-85
Identity = 162/326 (49.69%), Postives = 227/326 (69.63%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  PV++M+ IN AL   N L+K++L  G++ +VI  YR  IS LFL+PIA+FWERKTRP
Sbjct: 7   KWTPVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRP 66

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
            LT  IL  LF S+LVG +LT Y FL+GL YTSAT +CAF+++ P  TF++A++F +EK+
Sbjct: 67  TLTLNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKL 126

Query: 126 DMKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIG 185
           +MKSK G   V+G L+CI G L+L +YKG+PLT       +T+ +       +PE W+IG
Sbjct: 127 NMKSKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKL--ETHQLINNNHAMKPENWIIG 186

Query: 186 SLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVK 245
            ++L AG   + SW L+Q++V + YPCQYSST ++SFF  IQ  +L LI     + +++ 
Sbjct: 187 CVLLFAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILT 246

Query: 246 GKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHL 305
            K  I++++YAG+V  G+C VG SWC++K+GP+FT+ FTP   +FA +FDF ILH QI L
Sbjct: 247 DKLDIVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFL 306

Query: 306 GSVVGSVLIISGMYILLWGKDREAKE 332
           GSVVGS ++I G+YI L GK R  KE
Sbjct: 307 GSVVGSGVVIFGLYIFLLGKVRLMKE 330

BLAST of MS013435 vs. ExPASy Swiss-Prot
Match: Q9M129 (WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-73
Identity = 143/330 (43.33%), Postives = 216/330 (65.45%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  P++V++  N+    VN L+K++L  GI+ +VI  YR  IS LFL P+AYFWERKTRP
Sbjct: 7   KWAPMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRP 66

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
           +LT  I   LF+S+L G +L  Y +L+GL YTSAT   AF  ++P  TF++A++FG EK+
Sbjct: 67  KLTLSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKL 126

Query: 126 DMKSKGGRAKVLGTLVCISGNLILILYKGMPLT-SPGSATGKTNGVHPTVQPERPERWLI 185
            +K+K G   VLGTL+ + G L+L +Y+G+PLT SP  A    N           E W+ 
Sbjct: 127 SLKTKIGYGVVLGTLISLVGGLLLTMYQGIPLTNSPEQAANSNNHT-------GHENWIK 186

Query: 186 GSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVV 245
           G   L  G  ++SSW L+Q+++   YPC YSST I+S F  +Q  +L LI       +++
Sbjct: 187 GCFFLLTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWIL 246

Query: 246 KGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIH 305
           + +  I++++ AG V  G+C VGMSWC+K++GPV +++F+P + + A +FDF ILH +I+
Sbjct: 247 RDELTIITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIY 306

Query: 306 LGSVVGSVLIISGMYILLWGKDREAKECAV 335
           LGSV+GSV+++ G+YI LW + ++  EC +
Sbjct: 307 LGSVIGSVVVVIGLYIFLWSRSKQIVECKI 329

BLAST of MS013435 vs. ExPASy Swiss-Prot
Match: Q5XEZ0 (WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.3e-68
Identity = 142/326 (43.56%), Postives = 202/326 (61.96%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  PV+VM+  N+A+  VN L+K+ L  G++ +VI  YR  ISAL L P AY  ERKTRP
Sbjct: 13  KYSPVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRP 72

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
           Q+T  ++   F+S L+G +L  + FL+GL YTSAT SCA ++++P  TF LA++F  E V
Sbjct: 73  QITFRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENV 132

Query: 126 D-MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLI 185
             +K+K G  KV+GTL+CISG L L  YKG P  S   +       H     ++   WL+
Sbjct: 133 KILKTKAGMLKVIGTLICISGALFLTFYKG-PQISNSHSHSHGGASHNNNDQDKANNWLL 192

Query: 186 GSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVV 245
           G L LT G  + S W L Q  +   YPC+YSSTC+MS F+A Q  +L L      + +++
Sbjct: 193 GCLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWII 252

Query: 246 KGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIH 305
             +  I  +IYAG VG  +  V  +W +KK G VF +AF P   + A +FDF ILH  ++
Sbjct: 253 DDRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLY 312

Query: 306 LGSVVGSVLIISGMYILLWGKDREAK 331
           LGSV+GS++ I+G+Y+ LWGK++E +
Sbjct: 313 LGSVIGSLVTITGLYMFLWGKNKETE 337

BLAST of MS013435 vs. ExPASy Swiss-Prot
Match: Q9M131 (WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.5e-64
Identity = 137/335 (40.90%), Postives = 205/335 (61.19%), Query Frame = 0

Query: 3   KGAKMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERK 62
           K  +  PV+VML  ++A+  VN L+K+ L  G++ ++   YR  ISAL L P +Y WERK
Sbjct: 3   KEEQWAPVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERK 62

Query: 63  TRPQLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGM 122
           TRPQLT  +L   FIS L+G +L  + FL+GL YTSAT S A ++++P  TF LA++F +
Sbjct: 63  TRPQLTFMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRI 122

Query: 123 EKV-DMKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPER 182
           E   ++KSK G  KV+GTL+CI G ++L  YKG  L++P S     +  +     ++ ++
Sbjct: 123 ENAQNLKSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQTKK 182

Query: 183 WLIGSLVLTAGCFMWSSWFLMQSRVGKVYP-CQYSSTCIMSFFSAIQSVVLHLIIDTKNS 242
           WL+G L L  G  + S W L Q ++   YP  +YSSTC+MS F++ Q  +L L       
Sbjct: 183 WLLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVK 242

Query: 243 VFVVKGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILH 302
            ++++ K  IL  +YAG VG  +  V  SW +K  G VF + F+P   + A +FDF ILH
Sbjct: 243 DWIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILH 302

Query: 303 EQIHLGSVVGSVLIISGMYILLWGKDREAKECAVK 336
             ++LGS++GSV+ I+G+Y+ LWG+  E  +   K
Sbjct: 303 SPLYLGSILGSVVTITGLYVFLWGRKNETDQSVSK 337

BLAST of MS013435 vs. ExPASy TrEMBL
Match: A0A6J1CEV7 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111010859 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.7e-178
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD
Sbjct: 61  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
           KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG
Sbjct: 241 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SVVGSVLIISGMYILLWGKDREAKECAVK
Sbjct: 301 SVVGSVLIISGMYILLWGKDREAKECAVK 329

BLAST of MS013435 vs. ExPASy TrEMBL
Match: A0A6J1EHA3 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.0e-161
Identity = 298/328 (90.85%), Postives = 312/328 (95.12%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLSINLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSL+GLTLTLYLFLIGL YTSATFSCAFLNLVPVNTF+LAVL  MEKVD
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNLILILYKGM LTSPGSAT KTNG   TVQ + P +WLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
             AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 TFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAV 335
           SV+GSV+IISGMYILLWGKDREAKECAV
Sbjct: 301 SVIGSVMIISGMYILLWGKDREAKECAV 328

BLAST of MS013435 vs. ExPASy TrEMBL
Match: A0A6J1KGI9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.0e-161
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MKPVVVMLS+NLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSVNLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAYILFLLFISSL+GLTLTLYLFLIGL YTSATFSCAFLNLVPVNTF+LAVL  MEKVD
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGGRAKVLGTLVCISGNL+LILYKGMPLTSPGSAT K NG   TVQ E P +WLIGS
Sbjct: 121 MKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKINGARVTVQAETPGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLTAGCFMWSSWFLMQSRV KVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG
Sbjct: 181 LVLTAGCFMWSSWFLMQSRVCKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
             AILSL+YAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 241 TFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SV+GSV+IISGMYILLWGKDREAKECAVK
Sbjct: 301 SVIGSVMIISGMYILLWGKDREAKECAVK 329

BLAST of MS013435 vs. ExPASy TrEMBL
Match: A0A1S3BCC0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 9.2e-155
Identity = 288/329 (87.54%), Postives = 305/329 (92.71%), Query Frame = 0

Query: 7   MKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQ 66
           MK VVVMLSINLALAGVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQ
Sbjct: 1   MKAVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 67  LTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVD 126
           LTAY LFLLFISSL+GLTLTLYLFLIGL +TSATFSCAFLNLVPVNTF LAVLF MEKV+
Sbjct: 61  LTAYTLFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFFLAVLFRMEKVN 120

Query: 127 MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGS 186
           MKSKGG AKV+GTLVCISGNL+LILYKG PLTSPGS  GKTNG    V+ E   +WLIGS
Sbjct: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRAMVEAETQGKWLIGS 180

Query: 187 LVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKG 246
           LVLT GCFMWSSWFLMQ+RVGKVYPCQYSSTCIMSFFSAIQS VLHLIID KNS+F+VKG
Sbjct: 181 LVLTGGCFMWSSWFLMQTRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSLFIVKG 240

Query: 247 KSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLG 306
           K AILSLIYAGSVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHE IHLG
Sbjct: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEPIHLG 300

Query: 307 SVVGSVLIISGMYILLWGKDREAKECAVK 336
           SVVGSVLIISGMYILLWGKD+EAKEC VK
Sbjct: 301 SVVGSVLIISGMYILLWGKDKEAKECVVK 329

BLAST of MS013435 vs. ExPASy TrEMBL
Match: A0A6J1EGZ5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.6e-154
Identity = 283/317 (89.27%), Postives = 299/317 (94.32%), Query Frame = 0

Query: 18  LALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQLTAYILFLLFI 77
           + + GVNILLKEIL+EGISQL+IV+YRQTIS+LFL PIAYFWERKTRPQLTAYILFLLFI
Sbjct: 1   MTVKGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFI 60

Query: 78  SSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVDMKSKGGRAKVL 137
           SSL+GLTLTLYLFLIGL YTSATFSCAFLNLVPVNTF+LAVL  MEKVDMKSKGGRAKVL
Sbjct: 61  SSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDMKSKGGRAKVL 120

Query: 138 GTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGSLVLTAGCFMWS 197
           GTLVCISGNLILILYKGM LTSPGSAT KTNG   TVQ + P +WLIGSLVLTAGCFMWS
Sbjct: 121 GTLVCISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGSLVLTAGCFMWS 180

Query: 198 SWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKGKSAILSLIYAG 257
           SWFLMQSRVGKVYPCQYSSTCIMSFFSAIQS VLHLIID KNSVF+VKG  AILSL+YAG
Sbjct: 181 SWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAG 240

Query: 258 SVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLGSVVGSVLIISG 317
           SVGSGLCYVGMSWCVK+KGPVFTAAFTPFME+FAAIFDFFILHEQIHLGSV+GSV+IISG
Sbjct: 241 SVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISG 300

Query: 318 MYILLWGKDREAKECAV 335
           MYILLWGKDREAKECAV
Sbjct: 301 MYILLWGKDREAKECAV 317

BLAST of MS013435 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-86
Identity = 161/328 (49.09%), Postives = 232/328 (70.73%), Query Frame = 0

Query: 8   KPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRPQL 67
           K V++M  IN+ L+ VN++ K+++ EG++++V   YR  +  LFL P A F ER  RP+L
Sbjct: 10  KAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKL 69

Query: 68  TAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKVDM 127
           T  IL  LF S+L+G +L  Y FLIGL+YTS+TFS AF N+VP  TF LA++F  E +++
Sbjct: 70  TGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNI 129

Query: 128 KSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIGSL 187
           KS  GRAK+LGT++CI G L+L LYKG  L+   S   +T+           ++W +GS+
Sbjct: 130 KSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETH-TRTDSTGAMTQKWAMGSI 189

Query: 188 VLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVKGK 247
           +L     +WSSWF++Q+++ +VYPCQY+ST I+SFF  IQS +L LI +   S++VVK K
Sbjct: 190 MLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDK 249

Query: 248 SAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHLGS 307
             +L+L+Y+G VGSGLCYVGMSWC++++G VFT++F P +++FAAIF F  LHEQI+ GS
Sbjct: 250 FQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGS 309

Query: 308 VVGSVLIISGMYILLWGKDREAKECAVK 336
           V+GS++II G+YILLWGK ++      K
Sbjct: 310 VIGSMVIIVGLYILLWGKSKDKSASVTK 336

BLAST of MS013435 vs. TAIR 10
Match: AT4G01440.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.0e-86
Identity = 162/326 (49.69%), Postives = 227/326 (69.63%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  PV++M+ IN AL   N L+K++L  G++ +VI  YR  IS LFL+PIA+FWERKTRP
Sbjct: 7   KWTPVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRP 66

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
            LT  IL  LF S+LVG +LT Y FL+GL YTSAT +CAF+++ P  TF++A++F +EK+
Sbjct: 67  TLTLNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKL 126

Query: 126 DMKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLIG 185
           +MKSK G   V+G L+CI G L+L +YKG+PLT       +T+ +       +PE W+IG
Sbjct: 127 NMKSKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKL--ETHQLINNNHAMKPENWIIG 186

Query: 186 SLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVVK 245
            ++L AG   + SW L+Q++V + YPCQYSST ++SFF  IQ  +L LI     + +++ 
Sbjct: 187 CVLLFAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILT 246

Query: 246 GKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIHL 305
            K  I++++YAG+V  G+C VG SWC++K+GP+FT+ FTP   +FA +FDF ILH QI L
Sbjct: 247 DKLDIVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFL 306

Query: 306 GSVVGSVLIISGMYILLWGKDREAKE 332
           GSVVGS ++I G+YI L GK R  KE
Sbjct: 307 GSVVGSGVVIFGLYIFLLGKVRLMKE 330

BLAST of MS013435 vs. TAIR 10
Match: AT4G01450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 278.5 bits (711), Expect = 7.1e-75
Identity = 143/330 (43.33%), Postives = 216/330 (65.45%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  P++V++  N+    VN L+K++L  GI+ +VI  YR  IS LFL P+AYFWERKTRP
Sbjct: 7   KWAPMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRP 66

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
           +LT  I   LF+S+L G +L  Y +L+GL YTSAT   AF  ++P  TF++A++FG EK+
Sbjct: 67  KLTLSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKL 126

Query: 126 DMKSKGGRAKVLGTLVCISGNLILILYKGMPLT-SPGSATGKTNGVHPTVQPERPERWLI 185
            +K+K G   VLGTL+ + G L+L +Y+G+PLT SP  A    N           E W+ 
Sbjct: 127 SLKTKIGYGVVLGTLISLVGGLLLTMYQGIPLTNSPEQAANSNNHT-------GHENWIK 186

Query: 186 GSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVV 245
           G   L  G  ++SSW L+Q+++   YPC YSST I+S F  +Q  +L LI       +++
Sbjct: 187 GCFFLLTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWIL 246

Query: 246 KGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIH 305
           + +  I++++ AG V  G+C VGMSWC+K++GPV +++F+P + + A +FDF ILH +I+
Sbjct: 247 RDELTIITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIY 306

Query: 306 LGSVVGSVLIISGMYILLWGKDREAKECAV 335
           LGSV+GSV+++ G+YI LW + ++  EC +
Sbjct: 307 LGSVIGSVVVVIGLYIFLWSRSKQIVECKI 329

BLAST of MS013435 vs. TAIR 10
Match: AT1G01070.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.5e-69
Identity = 142/326 (43.56%), Postives = 202/326 (61.96%), Query Frame = 0

Query: 6   KMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERKTRP 65
           K  PV+VM+  N+A+  VN L+K+ L  G++ +VI  YR  ISAL L P AY  ERKTRP
Sbjct: 13  KYSPVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRP 72

Query: 66  QLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGMEKV 125
           Q+T  ++   F+S L+G +L  + FL+GL YTSAT SCA ++++P  TF LA++F  E V
Sbjct: 73  QITFRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENV 132

Query: 126 D-MKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPERWLI 185
             +K+K G  KV+GTL+CISG L L  YKG P  S   +       H     ++   WL+
Sbjct: 133 KILKTKAGMLKVIGTLICISGALFLTFYKG-PQISNSHSHSHGGASHNNNDQDKANNWLL 192

Query: 186 GSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSVVLHLIIDTKNSVFVV 245
           G L LT G  + S W L Q  +   YPC+YSSTC+MS F+A Q  +L L      + +++
Sbjct: 193 GCLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWII 252

Query: 246 KGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILHEQIH 305
             +  I  +IYAG VG  +  V  +W +KK G VF +AF P   + A +FDF ILH  ++
Sbjct: 253 DDRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLY 312

Query: 306 LGSVVGSVLIISGMYILLWGKDREAK 331
           LGSV+GS++ I+G+Y+ LWGK++E +
Sbjct: 313 LGSVIGSLVTITGLYMFLWGKNKETE 337

BLAST of MS013435 vs. TAIR 10
Match: AT4G01430.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.8e-65
Identity = 137/335 (40.90%), Postives = 205/335 (61.19%), Query Frame = 0

Query: 3   KGAKMKPVVVMLSINLALAGVNILLKEILSEGISQLVIVMYRQTISALFLSPIAYFWERK 62
           K  +  PV+VML  ++A+  VN L+K+ L  G++ ++   YR  ISAL L P +Y WERK
Sbjct: 3   KEEQWAPVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERK 62

Query: 63  TRPQLTAYILFLLFISSLVGLTLTLYLFLIGLQYTSATFSCAFLNLVPVNTFILAVLFGM 122
           TRPQLT  +L   FIS L+G +L  + FL+GL YTSAT S A ++++P  TF LA++F +
Sbjct: 63  TRPQLTFMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRI 122

Query: 123 EKV-DMKSKGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKTNGVHPTVQPERPER 182
           E   ++KSK G  KV+GTL+CI G ++L  YKG  L++P S     +  +     ++ ++
Sbjct: 123 ENAQNLKSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQTKK 182

Query: 183 WLIGSLVLTAGCFMWSSWFLMQSRVGKVYP-CQYSSTCIMSFFSAIQSVVLHLIIDTKNS 242
           WL+G L L  G  + S W L Q ++   YP  +YSSTC+MS F++ Q  +L L       
Sbjct: 183 WLLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVK 242

Query: 243 VFVVKGKSAILSLIYAGSVGSGLCYVGMSWCVKKKGPVFTAAFTPFMELFAAIFDFFILH 302
            ++++ K  IL  +YAG VG  +  V  SW +K  G VF + F+P   + A +FDF ILH
Sbjct: 243 DWIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILH 302

Query: 303 EQIHLGSVVGSVLIISGMYILLWGKDREAKECAVK 336
             ++LGS++GSV+ I+G+Y+ LWG+  E  +   K
Sbjct: 303 SPLYLGSILGSVVTITGLYVFLWGRKNETDQSVSK 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140124.11.6e-177100.00WAT1-related protein At3g30340-like isoform X1 [Momordica charantia][more]
XP_023520073.12.7e-16190.88WAT1-related protein At3g30340-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022927179.16.1e-16190.85WAT1-related protein At3g30340-like isoform X1 [Cucurbita moschata][more]
XP_023001437.11.0e-16090.27WAT1-related protein At3g30340-like isoform X1 [Cucurbita maxima][more]
XP_038895213.17.5e-15989.67WAT1-related protein At3g30340-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LI652.0e-8549.09WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
Q9M1305.7e-8549.69WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2... [more]
Q9M1291.0e-7343.33WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2... [more]
Q5XEZ06.3e-6843.56WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2... [more]
Q9M1312.5e-6440.90WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1CEV77.7e-178100.00WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111010859 PE=3 SV=1[more]
A0A6J1EHA33.0e-16190.85WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1[more]
A0A6J1KGI95.0e-16190.27WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1[more]
A0A1S3BCC09.2e-15587.54WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1[more]
A0A6J1EGZ54.6e-15489.27WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30340.11.4e-8649.09nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01440.14.0e-8649.69nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01450.27.1e-7543.33nodulin MtN21 /EamA-like transporter family protein [more]
AT1G01070.14.5e-6943.56nodulin MtN21 /EamA-like transporter family protein [more]
AT4G01430.11.8e-6540.90nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 14..129
e-value: 1.6E-14
score: 54.2
coord: 184..322
e-value: 5.0E-14
score: 52.6
NoneNo IPR availablePANTHERPTHR31218:SF301WAT1-RELATED PROTEINcoord: 9..330
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 185..327
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 43..153
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013435.1MS013435.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity