MS013317 (gene) Bitter gourd (TR) v1

Overview
NameMS013317
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAnkyrin repeat-containing protein
Locationscaffold402: 284567 .. 287651 (+)
RNA-Seq ExpressionMS013317
SyntenyMS013317
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCTTGGACGAGATGCGAAGCGCTTACAGGACTGCGATAAAAGGGAAATGGAAGAATCTGCCTCAAGAAATATTGATCCACAAGGCGAAAATCATGTTTCCCATGACAACCGGCGGAGACACGGCGATGCATCTCGCCGTGTACAGCAGAAAAGAAGAGCCGACGAAATCTCTGCTGGCGGCGACCTCAGAGGTGGAGAGAGAGTTCTGGACGAATAGTGCTGGAAATACGCCGTTGCACGAAGCTGCCGCCGTCGGGAATCTGGCTGCCGTCAAGCTTCTGGTGGAGTACAAAAAAGAGGATCTGTTGGCGCAGAACATTTACGGCGAAACGCCTCTGTTCAGAGCGGTCAGATTCGGACACCTTCACATCGTCGAGTACATTCTGGAAGATTGTGAAGATTACTTTTCTCGATCGCCGATAAATTGGACCAAGGAGAAGGAATTTCCGATCATTCACGCGGCTATTCAGAGCGAGAAATTCGGTTCGTCTCTGTTCTTTTTTTTTTTTTTTACGTTTTTTCTTTTGATCTCTGAGTCTAGGTTTTCTGATCCGCCATTGATGCGCGGTTATAGATGTGGTTCTGAGACTGGTTGATTTCGATAAATCGCTGTTGGAGATGAAGGATGAAGACGGCAAAACGGCTCTCTATGTGCTCGCAAACATGCCACTCATTTTTCGAAGCGGATCCTCCATGGGATCCTTGGAAAAAATTATCTATGCGGGTAAGAATTCTCACTCCCTTGCTCAAGAAAACATTCAATCGATATTCCTTTGGATTAATAGGAAGAAAAATGGAGTCAATTTTTTTACGTTGAAATTTTAATTTTATCAAAATGGATTCCGGACTAGATGTGGAATAACTTGAATTTGGATATTTCTTTACCTTATTTGTGTCCAGATTTGGTTATTTATCTAAATAAATTTAGATTATATTGAGTCCTTCTGTGGATTTAATTTTGAACAATTTATTAGTCCGTTACATCCGTTGATGAGTTTTTAAACTTGGTTTTAAGACTGTATTTATTGATTTTCTTTGAGCATAATTCATTTTCGTATTTTCGTATTTTCTTTCAACAGTGCTCCCCAGTGAAGATATTTATAAATATAATTTTAAAACTTTTGGATCTTCCAACAAAGATGACTCTGAAAGCTCCGGTGCTTTGAACAACAAGAAGAATGAAGATTTAGAAGCACAACATAATCCTGATTATGGACTTAATTTTTGCCGTTCAAATTGTTGTAAGTTTCTAAATTTTACAGACTAAGCTACTAAATTGATTAATTGACCAATTGATTTTTCTCTATATGTATATATTGAAATAAATACTGAAATGTGCCATATTAAGAGGTTAACTTAGAAATTTAGGACCCTTTAATAACTATTTCATGTTCTATTTTGATTTTTGGTTTTTAATTTTTCTTTTAAAAAATGGTTTCATAAGCAATTCAATTTTCAATTTTTGATTTTTAAAAACAGATTTAAAAAAGAAAAGATTAATTTTCAAAAACTGATTTCTGTTTTTAAAATTTAATTTTTTTCTTTTAATACTCAACTTTTTTTTTTTTCTTTTCTCCTACTTATCTGTAACAAAATTCTATGCCAACTACTGGCATCTGGGATCCAATTTCTGAATTCACTCATGAAATATTTTCCTTATCACACACCAAGAAAAAAGAAAAAAAAATATAATTCTATATCTTTGTGGCAGGGAAATATTTTGTACACTTTTTGACGTGCTACTTCTGGAGATTCATCTTTCTAGGTTTCACTCTCTCTCTTCTTTCTCTCTCCTCTGCTTTGCTCTGCAGCTCTCTGATTCTGATTCTGAATTGTGAAACTTGTATAGGGTGGCCGCAATGGAAACAACTGTACCGGAAAAAGCAGCACCACAAATTGGCCGTAACCATCACCAAGATGCTTGCTGAGATCGATTATTCATGGCGCAACACCGATTCGACGCCGGAGATCAACGAAATCGACTACGGAGCCCGTCGCGGAGAACGTAAACAGATAGAAGAAGGATCCCTCCTCATCAACAAGATCCAAACGGAGGAGGACGACAAATCGAAAAGTAAAGACATCGACTACGACGATATCCACGAAACTCCGCTCCTCCTCGCCGCCGCCAACGGAATTATCGAGATCGTCGAGGCGATCGTCGACGCGCATCCTCCGGCTGTCGATTATGTGACGAAGAAGCAGAGGAACGTCCTGCACGTGGCGATCGCGCATCGCCAGAGTCAGATCTTGGACTGGATCGAGAAAGAAGAATTGGTAATGGCGAGGCTCGTCACGCGAATCGACGCGTCAGGGTTCACAGCTCTGCACCACGCCGGAATCACGAAATTCAGTCGGGGAGGGTCTTACGGCCCTGCTCTGCAACTCCAGGAGGAGCTCAAATGGTTCCATCGAGTGCAGGATCAGATCCCGACGCTCTACGCGAAGCACCACAGCCAGAACGAAGGGACTGCTCGACAGCACTTCGACAATCAGCACCAGAAAATGCTCGAGGACGGAAAAGAATGGCTGAAGAAAACCTCCGAGTCCTGCTCCGCCGTCGCGGTCCTAATCGCCACCGTCGTCTTCGCCGCCGCCTACACCGTCCCCGGCGGCCTCAACAGCAAGACCGGCTCCCCGGTGCTCGTCACGGATCCGATCTACATGGTGTTCACGGTGATGGACATCGTCGGGCTGGCCACGTCGCTGACGTCGGTGGTTCTGTTTCTGTCGATTCTGACTTCGCCGTTTATGATGCATGATTTTCTGCACGCGCTGCCGCTGAAATTGTTGTTCGGATTTCAGCTGCTGTTCTTATCGGTGGCGAGCACGATGATGGCGTTCGCGCTGACGATCGTGCTGACGATGAAATCGGCGGAGATAAAATGGACGATGAGTTTACTGTATATGGCGTCATTTTTTCCGGTGACGATGTTCATAATCATGCAGCTGCCGTTGTATGTGGAACTGGTGAAGAGTATTTGGAGCTTTCGCCACAATCTTTTCCAGTTCCTTCCCATTGGTTTCGTCACCCCATTTTGGATGCTTCCTTCCAAAATTGTTACCAGGAAATTTGCT

mRNA sequence

ATGGATTTCTTGGACGAGATGCGAAGCGCTTACAGGACTGCGATAAAAGGGAAATGGAAGAATCTGCCTCAAGAAATATTGATCCACAAGGCGAAAATCATGTTTCCCATGACAACCGGCGGAGACACGGCGATGCATCTCGCCGTGTACAGCAGAAAAGAAGAGCCGACGAAATCTCTGCTGGCGGCGACCTCAGAGGTGGAGAGAGAGTTCTGGACGAATAGTGCTGGAAATACGCCGTTGCACGAAGCTGCCGCCGTCGGGAATCTGGCTGCCGTCAAGCTTCTGGTGGAGTACAAAAAAGAGGATCTGTTGGCGCAGAACATTTACGGCGAAACGCCTCTGTTCAGAGCGGTCAGATTCGGACACCTTCACATCGTCGAGTACATTCTGGAAGATTGTGAAGATTACTTTTCTCGATCGCCGATAAATTGGACCAAGGAGAAGGAATTTCCGATCATTCACGCGGCTATTCAGAGCGAGAAATTCGATGTGGTTCTGAGACTGGTTGATTTCGATAAATCGCTGTTGGAGATGAAGGATGAAGACGGCAAAACGGCTCTCTATGTGCTCGCAAACATGCCACTCATTTTTCGAAGCGGATCCTCCATGGGATCCTTGGAAAAAATTATCTATGCGGTGCTCCCCAGTGAAGATATTTATAAATATAATTTTAAAACTTTTGGATCTTCCAACAAAGATGACTCTGAAAGCTCCGGTGCTTTGAACAACAAGAAGAATGAAGATTTAGAAGCACAACATAATCCTGATTATGGACTTAATTTTTGCCGTTCAAATTGTTGGAAATATTTTGTACACTTTTTGACGTGCTACTTCTGGAGATTCATCTTTCTAGGTTTCACTCTCTCTCTTCTTTCTCTCTCCTCTGCTTTGCTCTGCAGCTCTCTGATTCTGATTCTGAATTGTGAAACTTGTATAGGGTGGCCGCAATGGAAACAACTGTACCGGAAAAAGCAGCACCACAAATTGGCCGTAACCATCACCAAGATGCTTGCTGAGATCGATTATTCATGGCGCAACACCGATTCGACGCCGGAGATCAACGAAATCGACTACGGAGCCCGTCGCGGAGAACGTAAACAGATAGAAGAAGGATCCCTCCTCATCAACAAGATCCAAACGGAGGAGGACGACAAATCGAAAAGTAAAGACATCGACTACGACGATATCCACGAAACTCCGCTCCTCCTCGCCGCCGCCAACGGAATTATCGAGATCGTCGAGGCGATCGTCGACGCGCATCCTCCGGCTGTCGATTATGTGACGAAGAAGCAGAGGAACGTCCTGCACGTGGCGATCGCGCATCGCCAGAGTCAGATCTTGGACTGGATCGAGAAAGAAGAATTGGTAATGGCGAGGCTCGTCACGCGAATCGACGCGTCAGGGTTCACAGCTCTGCACCACGCCGGAATCACGAAATTCAGTCGGGGAGGGTCTTACGGCCCTGCTCTGCAACTCCAGGAGGAGCTCAAATGGTTCCATCGAGTGCAGGATCAGATCCCGACGCTCTACGCGAAGCACCACAGCCAGAACGAAGGGACTGCTCGACAGCACTTCGACAATCAGCACCAGAAAATGCTCGAGGACGGAAAAGAATGGCTGAAGAAAACCTCCGAGTCCTGCTCCGCCGTCGCGGTCCTAATCGCCACCGTCGTCTTCGCCGCCGCCTACACCGTCCCCGGCGGCCTCAACAGCAAGACCGGCTCCCCGGTGCTCGTCACGGATCCGATCTACATGGTGTTCACGGTGATGGACATCGTCGGGCTGGCCACGTCGCTGACGTCGGTGGTTCTGTTTCTGTCGATTCTGACTTCGCCGTTTATGATGCATGATTTTCTGCACGCGCTGCCGCTGAAATTGTTGTTCGGATTTCAGCTGCTGTTCTTATCGGTGGCGAGCACGATGATGGCGTTCGCGCTGACGATCGTGCTGACGATGAAATCGGCGGAGATAAAATGGACGATGAGTTTACTGTATATGGCGTCATTTTTTCCGGTGACGATGTTCATAATCATGCAGCTGCCGTTGTATGTGGAACTGGTGAAGAGTATTTGGAGCTTTCGCCACAATCTTTTCCAGTTCCTTCCCATTGGTTTCGTCACCCCATTTTGGATGCTTCCTTCCAAAATTGTTACCAGGAAATTTGCT

Coding sequence (CDS)

ATGGATTTCTTGGACGAGATGCGAAGCGCTTACAGGACTGCGATAAAAGGGAAATGGAAGAATCTGCCTCAAGAAATATTGATCCACAAGGCGAAAATCATGTTTCCCATGACAACCGGCGGAGACACGGCGATGCATCTCGCCGTGTACAGCAGAAAAGAAGAGCCGACGAAATCTCTGCTGGCGGCGACCTCAGAGGTGGAGAGAGAGTTCTGGACGAATAGTGCTGGAAATACGCCGTTGCACGAAGCTGCCGCCGTCGGGAATCTGGCTGCCGTCAAGCTTCTGGTGGAGTACAAAAAAGAGGATCTGTTGGCGCAGAACATTTACGGCGAAACGCCTCTGTTCAGAGCGGTCAGATTCGGACACCTTCACATCGTCGAGTACATTCTGGAAGATTGTGAAGATTACTTTTCTCGATCGCCGATAAATTGGACCAAGGAGAAGGAATTTCCGATCATTCACGCGGCTATTCAGAGCGAGAAATTCGATGTGGTTCTGAGACTGGTTGATTTCGATAAATCGCTGTTGGAGATGAAGGATGAAGACGGCAAAACGGCTCTCTATGTGCTCGCAAACATGCCACTCATTTTTCGAAGCGGATCCTCCATGGGATCCTTGGAAAAAATTATCTATGCGGTGCTCCCCAGTGAAGATATTTATAAATATAATTTTAAAACTTTTGGATCTTCCAACAAAGATGACTCTGAAAGCTCCGGTGCTTTGAACAACAAGAAGAATGAAGATTTAGAAGCACAACATAATCCTGATTATGGACTTAATTTTTGCCGTTCAAATTGTTGGAAATATTTTGTACACTTTTTGACGTGCTACTTCTGGAGATTCATCTTTCTAGGTTTCACTCTCTCTCTTCTTTCTCTCTCCTCTGCTTTGCTCTGCAGCTCTCTGATTCTGATTCTGAATTGTGAAACTTGTATAGGGTGGCCGCAATGGAAACAACTGTACCGGAAAAAGCAGCACCACAAATTGGCCGTAACCATCACCAAGATGCTTGCTGAGATCGATTATTCATGGCGCAACACCGATTCGACGCCGGAGATCAACGAAATCGACTACGGAGCCCGTCGCGGAGAACGTAAACAGATAGAAGAAGGATCCCTCCTCATCAACAAGATCCAAACGGAGGAGGACGACAAATCGAAAAGTAAAGACATCGACTACGACGATATCCACGAAACTCCGCTCCTCCTCGCCGCCGCCAACGGAATTATCGAGATCGTCGAGGCGATCGTCGACGCGCATCCTCCGGCTGTCGATTATGTGACGAAGAAGCAGAGGAACGTCCTGCACGTGGCGATCGCGCATCGCCAGAGTCAGATCTTGGACTGGATCGAGAAAGAAGAATTGGTAATGGCGAGGCTCGTCACGCGAATCGACGCGTCAGGGTTCACAGCTCTGCACCACGCCGGAATCACGAAATTCAGTCGGGGAGGGTCTTACGGCCCTGCTCTGCAACTCCAGGAGGAGCTCAAATGGTTCCATCGAGTGCAGGATCAGATCCCGACGCTCTACGCGAAGCACCACAGCCAGAACGAAGGGACTGCTCGACAGCACTTCGACAATCAGCACCAGAAAATGCTCGAGGACGGAAAAGAATGGCTGAAGAAAACCTCCGAGTCCTGCTCCGCCGTCGCGGTCCTAATCGCCACCGTCGTCTTCGCCGCCGCCTACACCGTCCCCGGCGGCCTCAACAGCAAGACCGGCTCCCCGGTGCTCGTCACGGATCCGATCTACATGGTGTTCACGGTGATGGACATCGTCGGGCTGGCCACGTCGCTGACGTCGGTGGTTCTGTTTCTGTCGATTCTGACTTCGCCGTTTATGATGCATGATTTTCTGCACGCGCTGCCGCTGAAATTGTTGTTCGGATTTCAGCTGCTGTTCTTATCGGTGGCGAGCACGATGATGGCGTTCGCGCTGACGATCGTGCTGACGATGAAATCGGCGGAGATAAAATGGACGATGAGTTTACTGTATATGGCGTCATTTTTTCCGGTGACGATGTTCATAATCATGCAGCTGCCGTTGTATGTGGAACTGGTGAAGAGTATTTGGAGCTTTCGCCACAATCTTTTCCAGTTCCTTCCCATTGGTTTCGTCACCCCATTTTGGATGCTTCCTTCCAAAATTGTTACCAGGAAATTTGCT

Protein sequence

MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSLLAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVRFGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMKDEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSGALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLCSSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYGARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGITKFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATSLTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIKWTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVTRKFA
Homology
BLAST of MS013317 vs. NCBI nr
Match: XP_038894049.1 (uncharacterized protein LOC120082801 isoform X1 [Benincasa hispida])

HSP 1 Score: 880.9 bits (2275), Expect = 7.3e-252
Identity = 466/725 (64.28%), Postives = 553/725 (76.28%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           MD L+ MR  YR  IK +WK + +E L  K KI+FPMT  GDTA+HLAVYS +EEP ++L
Sbjct: 1   MDILEIMRVPYRAVIKEEWKKVKEE-LKDKTKIVFPMTASGDTAVHLAVYSGEEEPLRAL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           L   SE++  FW NSAGNTPLHEAA VGNLAAVKLLVEY K+DL+ +NIYGETPLFRA R
Sbjct: 61  LGEISEMDEAFWRNSAGNTPLHEAATVGNLAAVKLLVEYDKDDLVGENIYGETPLFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHLHIV Y+LEDCED FSRS  NWT +K  P+IHAAIQS+KF+V L+L++FDKSLLEM 
Sbjct: 121 CGHLHIVNYMLEDCEDLFSRSSRNWTTKKGNPLIHAAIQSQKFEVALKLIEFDKSLLEMT 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL+VLANMP  FRSG SM   E IIY +LPSEDIYK+++  F SS KD+   S 
Sbjct: 181 DPEGKTALHVLANMPFAFRSGYSMKFFESIIYTLLPSEDIYKFDYSKFVSSKKDNGSKSS 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            + N KN+DLEA +N     NF  SNCW Y +HFLT  FWRFIFL               
Sbjct: 241 IIIN-KNDDLEAGNNSSPPHNFWHSNCWLYLLHFLTGIFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDST-PEINEIDY 360
                        GWPQWK LY KKQ H+LA+TITKMLA +D+SWR T  T PE  E+D 
Sbjct: 301 -------------GWPQWKVLYEKKQQHRLALTITKMLAHVDFSWRQTQLTPPENTEVD- 360

Query: 361 GARRGERKQIEEGSLLINKIQTE---EDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEA 420
               G  +  E   + +N ++T+   + ++ + +DI+Y D HETPLLLAAANGIIEIV+ 
Sbjct: 361 --SLGIHRPNEGNGVNLNILRTQPSSDQNQGEVEDIEYYDHHETPLLLAAANGIIEIVQQ 420

Query: 421 IVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHA 480
           IV+ +P AVDY+T  QRNVLHVAIA+RQ  + +WI+   L+M RLVTRIDA GFTALHH 
Sbjct: 421 IVEVYPQAVDYLTVHQRNVLHVAIAYRQKTVFNWIQNHRLIMTRLVTRIDALGFTALHHV 480

Query: 481 GITKFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLED 540
           GITKF RGG++GPALQLQ ELKW+ RVQ +IP LY  HH+  + TAR+ F   H+KMLED
Sbjct: 481 GITKFYRGGTHGPALQLQHELKWYERVQSEIPALYNMHHNSMKWTAREFFYKTHEKMLED 540

Query: 541 GKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGL 600
            KEWLKKTSESCSAVAVL+ATVVFAAAYTVPGGLNS+TGSPVL+T+PIY+VFT+MDIV L
Sbjct: 541 AKEWLKKTSESCSAVAVLVATVVFAAAYTVPGGLNSQTGSPVLLTEPIYIVFTIMDIVAL 600

Query: 601 ATSLTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSA 660
           AT+LTSVVLFLSILTS F M DFLHALPLKL  GFQLLF SVASTMMAFALTIVLT+KS 
Sbjct: 601 ATALTSVVLFLSILTSSFKMEDFLHALPLKLSIGFQLLFFSVASTMMAFALTIVLTVKSE 660

Query: 661 EIKWTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSK 720
           E+KWT+SLLY+A+FFPVTMFII+QLPLYVELVK+IWS+RHN+ +FLP+GF+  FW LPSK
Sbjct: 661 EMKWTVSLLYIATFFPVTMFIIIQLPLYVELVKNIWSYRHNITKFLPMGFLALFWKLPSK 692

Query: 721 IVTRK 722
             +RK
Sbjct: 721 TFSRK 692

BLAST of MS013317 vs. NCBI nr
Match: XP_022975228.1 (uncharacterized protein LOC111474320 [Cucurbita maxima])

HSP 1 Score: 865.5 bits (2235), Expect = 3.2e-247
Identity = 464/723 (64.18%), Postives = 542/723 (74.97%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WKN+ +E L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSLFDVMRGLYKAVIKEEWKNVEEE-LKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA S V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETP+FRA R
Sbjct: 61  LAAISTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPMFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IVE+ILEDC+DY SRSP NWT     PIIHA IQS+KFDVVL+L +FDKSLLEMK
Sbjct: 121 CGHLDIVEFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVVLKLAEFDKSLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL F SGSS+   E  IY +LP+EDIY Y    F SS    S+S  
Sbjct: 181 DSEGKTALQVLANMPLAFHSGSSLTFFESFIYTLLPNEDIYNYKPSNFESSKNHASQS-- 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YFV  LT  FWRFIFL               
Sbjct: 241 -LENKKNGDLEAGTNSNGTPNF-----WIYFVGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQT-EEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G  +  E   L I +IQ+   +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -STGISRSDENEKLDILQIQSIPINNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T   RN+LHVAIAHR+  I DWI K  L+MARLV RID  GFTALHH GIT
Sbjct: 421 ANPQAVDYLTVHDRNILHVAIAHRRKNIFDWILKRRLIMARLVNRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+ + TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQSQIPALYTMHHSKMKWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNSKTGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSKTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F M DFLH LPLKL  GFQLLFLSVA+TMMAFALTIVLTMK+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKMDDFLHTLPLKLSMGFQLLFLSVATTMMAFALTIVLTMKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYV+LVK+IW +RHNL +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVQLVKNIWGYRHNLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. NCBI nr
Match: XP_022974704.1 (uncharacterized protein LOC111473443 [Cucurbita maxima])

HSP 1 Score: 862.8 bits (2228), Expect = 2.1e-246
Identity = 463/723 (64.04%), Postives = 541/723 (74.83%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WKN+ +E L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSLFDVMRGLYKAVIKEEWKNVEEE-LKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA S V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETP+FRA R
Sbjct: 61  LAAISTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPMFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IVE+ILEDC+DY SRSP NWT     PIIHA IQS+KFDVVL+L +FDKSLLEMK
Sbjct: 121 CGHLDIVEFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVVLKLAEFDKSLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL F SGSS+   E  IY +LP+EDIY Y    F SS    S+S  
Sbjct: 181 DSEGKTALQVLANMPLAFHSGSSLTFFESFIYTLLPNEDIYNYKPSNFESSKNHASQS-- 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YFV  LT  FWRFIFL               
Sbjct: 241 -LENKKNGDLEAGTNSNGTPNF-----WIYFVGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQT-EEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G  +  E   L I +IQ+   +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -STGISRSDENEKLDILQIQSIPINNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T   RN+LHVAIAHR+  I DWI K  L+MARLV RID  GFTALHH GIT
Sbjct: 421 ANPLAVDYLTVHDRNILHVAIAHRRKNIFDWILKRRLIMARLVNRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+   TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQSQIPALYTMHHSKMRWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNSKTGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSKTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F + DFLH LPLKL  GFQLLFLSVA+TMMAFALTIVLTMK+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKIDDFLHTLPLKLSMGFQLLFLSVATTMMAFALTIVLTMKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYV+LVK+IW +RHNL +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVQLVKNIWGYRHNLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. NCBI nr
Match: XP_031739511.1 (uncharacterized protein LOC101203890 [Cucumis sativus])

HSP 1 Score: 844.3 bits (2180), Expect = 7.6e-241
Identity = 449/726 (61.85%), Postives = 540/726 (74.38%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           MDFL  M   YR  IK +WK + +E L  + KI+FPMT   DTA+HLAVYS  EEP ++ 
Sbjct: 1   MDFLVTMIEPYRAVIKEEWKKVKEE-LKDETKIVFPMTASRDTALHLAVYSGGEEPLRTF 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           L    E++  FW NSAGNTPLHEAA VGNLAAVKLLVEYKKEDL+A+NIYGETPLFRA R
Sbjct: 61  LVGIFEMDEAFWRNSAGNTPLHEAATVGNLAAVKLLVEYKKEDLVAENIYGETPLFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IV YILEDCED+FSR   +WT  K  PIIHAAIQS+KFDVVL+L +FDKSLLEM 
Sbjct: 121 CGHLEIVNYILEDCEDFFSRCSRHWTNRKGNPIIHAAIQSQKFDVVLKLTEFDKSLLEMT 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKD-DSESS 240
           + +GKTAL+VLANMP  F+SG  M   E IIY +LP++DIY Y +  FGSSN D +  S 
Sbjct: 181 NLEGKTALHVLANMPSAFQSGYPMKFFESIIYNLLPTQDIYNYKYSNFGSSNNDPNGYSK 240

Query: 241 GALNNKKNEDLEAQ-HNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSAL 300
            ++   KNEDLEA   N +     C SNCW + VHFL C+FW+FIFL             
Sbjct: 241 SSIIQNKNEDLEAGISNSETPNKLCHSNCWIHLVHFLACFFWKFIFL------------- 300

Query: 301 LCSSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDST-PEINEI 360
                          GWPQWK+LY KKQ HKLA+TIT MLA ID+SWR T +T PEI E+
Sbjct: 301 ---------------GWPQWKELYEKKQQHKLALTITNMLAHIDFSWRQTQATPPEITEV 360

Query: 361 D-YGARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEA 420
           D +G    +   ++   L  +   + + ++ +  DI++ + HETPLLLAAANGIIEIV+ 
Sbjct: 361 DSFGLPLPKEGDVDLHILTTH--PSSDHNQGEIGDIEFYEHHETPLLLAAANGIIEIVQQ 420

Query: 421 IVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHA 480
           IV+  P AVDYVT  QRN+LHVAIAHRQ Q+  WI+   L+M RLVTRID  GFTALHH 
Sbjct: 421 IVEVFPQAVDYVTVHQRNLLHVAIAHRQKQVFKWIQNHRLIMTRLVTRIDTLGFTALHHV 480

Query: 481 GITKFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLED 540
           G+TKF RGG++GPALQLQ+EL W+ RVQ QIP LY  HH++ + T R++F   H+KML+ 
Sbjct: 481 GVTKFFRGGTHGPALQLQQELIWYERVQSQIPALYNMHHNKMKWTPREYFYKTHEKMLDQ 540

Query: 541 GKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGL 600
            KEWLKKTSESCSAVAVL+ATVVFAAAYTVPGGLNSKTGSP+L+T+PIY+VFTVMDI+ L
Sbjct: 541 AKEWLKKTSESCSAVAVLVATVVFAAAYTVPGGLNSKTGSPILLTEPIYVVFTVMDILAL 600

Query: 601 ATSLTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSA 660
           AT+L+SVVLFLSILTS F M DFLH LP+KL  GFQLLF SVASTMMAFALTIVLT+KS 
Sbjct: 601 ATALSSVVLFLSILTSSFKMEDFLHTLPMKLSIGFQLLFFSVASTMMAFALTIVLTVKSE 660

Query: 661 EIKWTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSK 720
           E+KWT+SLLYMA+FFPVTMFII+QLPLYVELVK+IW + H + +FLP+GF+     LPSK
Sbjct: 661 EMKWTVSLLYMATFFPVTMFIIIQLPLYVELVKNIWRYGHKISKFLPMGFLALCCKLPSK 695

Query: 721 IVTRKF 723
           +++RKF
Sbjct: 721 VLSRKF 695

BLAST of MS013317 vs. NCBI nr
Match: XP_022940456.1 (uncharacterized protein LOC111446049 [Cucurbita moschata])

HSP 1 Score: 842.0 bits (2174), Expect = 3.7e-240
Identity = 455/723 (62.93%), Postives = 539/723 (74.55%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WK++ QE L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSPFDVMRGLYKAVIKEEWKSV-QEELKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA + V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETPLFRA R
Sbjct: 61  LAAITTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPLFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IV++ILEDC+DY SRSP NWT     PIIHA IQS+KFDV L+L +FD+SLLEMK
Sbjct: 121 CGHLDIVKFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVALKLAEFDESLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL FRSGSS    E  IY +LPSEDIY Y      +S    + +S 
Sbjct: 181 DSEGKTALQVLANMPLAFRSGSSPTFFESFIYTLLPSEDIYHYK---PSNSESSKNHASC 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YF   LT  FWRFIFL               
Sbjct: 241 CLKNKKNGDLEAGTNSNRPPNF-----WIYFGGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQTEE-DDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G     E   L I +IQ++  +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -SIGISLSDENEKLDILEIQSKPVNNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T + RN+LHVAIAHR+  I DWI K  L+MARLV+RID  GFTALHH GIT
Sbjct: 421 ANPQAVDYLTVQDRNILHVAIAHRRKNIFDWILKRRLIMARLVSRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+ + TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQTQIPALYTMHHSKMKWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNS TGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSNTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F M  FL+ LPLKL  GFQLLFLSVA+TMMAFALTIVLT+K+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKMDAFLYTLPLKLSMGFQLLFLSVATTMMAFALTIVLTVKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYVELVK+IW +RH+L +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVELVKNIWGYRHSLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. ExPASy Swiss-Prot
Match: Q9ET47 (Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 6.2e-12
Identity = 53/156 (33.97%), Postives = 79/156 (50.64%), Query Frame = 0

Query: 41  GDTAMHLAVYSRKEEPTKSLL---AATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLV 100
           G T +HLA      +  K LL    A S +     T   G  P+H AAA G+L ++KLLV
Sbjct: 104 GATVLHLAARFGHPDVVKWLLYQGGANSAI-----TTDTGALPIHYAAAKGDLPSLKLLV 163

Query: 101 EYKKEDLLAQNIYGETPLFRAVRFGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAA 160
            +  E + AQ   G TPL+ A + GHL + +Y++++C    S  P    ++   P +HAA
Sbjct: 164 GHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----SADPHLRAQDGMTP-LHAA 223

Query: 161 IQSEKFDVVLRLVDFDKSLLEMKDEDGKTALYVLAN 194
            Q     V++ LV F       +D DG TA++  A+
Sbjct: 224 AQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAAS 249

BLAST of MS013317 vs. ExPASy Swiss-Prot
Match: Q63618 (Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 9.0e-11
Identity = 53/156 (33.97%), Postives = 79/156 (50.64%), Query Frame = 0

Query: 41  GDTAMHLAVYSRKEEPTKSLL---AATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLV 100
           G T +HLA      +    LL    A S +     T   G  P+H AAA G+L ++KLLV
Sbjct: 104 GATVLHLAARFGHPDVVNWLLYQGGANSAI-----TTDTGALPIHYAAAKGDLPSMKLLV 163

Query: 101 EYKKEDLLAQNIYGETPLFRAVRFGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAA 160
            +  E + AQ   G TPL+ A + GHL + +Y++++C    S  P    ++   P +HAA
Sbjct: 164 GHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----SADPHLRAQDGMTP-LHAA 223

Query: 161 IQSEKFDVVLRLVDFDKSLLEMKDEDGKTALYVLAN 194
            Q     V++ LV F     E +D DG TA++  A+
Sbjct: 224 AQMGHNPVLVWLVSFADVSFE-QDHDGATAMHFAAS 248

BLAST of MS013317 vs. ExPASy Swiss-Prot
Match: B1AK53 (Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-10
Identity = 41/118 (34.75%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 77  GNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVRFGHLHIVEYILEDC-E 136
           G  P+H AAA G+  +++LLVE+  E + AQ   G TPL+ A + GHL + +Y++++C  
Sbjct: 138 GALPIHYAAAKGDFPSLRLLVEHYPEGVNAQTKNGATPLYLACQEGHLEVTQYLVQECGA 197

Query: 137 DYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMKDEDGKTALYVLAN 194
           D  +R+    T       +HAA Q     V++ LV      L  +D+DG TA++  A+
Sbjct: 198 DPHARAHDGMTP------LHAAAQMGHSPVIVWLVSCTDVSLSEQDKDGATAMHFAAS 249

BLAST of MS013317 vs. ExPASy Swiss-Prot
Match: C7B178 (Protein VAPYRIN OS=Petunia hybrida OX=4102 GN=VPY PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.9e-08
Identity = 53/186 (28.49%), Postives = 89/186 (47.85%), Query Frame = 0

Query: 25  EILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSLLAATSEVEREFWTNSAGNTPLHEA 84
           ++L+ K   +  +T  G+TA+HLAV  R+ +  + LLA  +    +  +   G+TPLH A
Sbjct: 256 KLLLLKGANVNSLTKDGNTALHLAVEERRRDCARLLLA--NGARADICSTGNGDTPLHIA 315

Query: 85  AAVGNLAAVKLLVEYKKEDLLAQNIYGET-----------PLFRAVRF----------GH 144
           A +G+   V++L++ K  +   +N YG+T            LF A+R           G 
Sbjct: 316 AGLGDEHMVRVLLQ-KGAEKYIRNKYGKTAYDVAAEHGHNKLFDALRLGDSLCVAARKGE 375

Query: 145 LHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMKDED 190
           +  V+ +LE+      R    WT       +H A    + +VV  L+D +   +  +DED
Sbjct: 376 VRTVQRLLENGASINGRDQHGWT------ALHRACFKGRIEVVKALID-NGIDVNARDED 431

BLAST of MS013317 vs. ExPASy Swiss-Prot
Match: Q3UYR4 (Espin-like protein OS=Mus musculus OX=10090 GN=Espnl PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.2e-08
Identity = 35/116 (30.17%), Postives = 57/116 (49.14%), Query Frame = 0

Query: 77  GNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVRFGHLHIVEYILEDCED 136
           G  PLH AA  G+L  +KLL       +  +   G +PL+ A + GHLH+ +++++DC  
Sbjct: 137 GALPLHHAAVSGDLTCLKLLTAAHSSGVNQRTCSGASPLYLACQEGHLHLAQFLVKDC-- 196

Query: 137 YFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMKDEDGKTALYVLA 193
               + +          +HAA     + +V+ LV F    L  +D +G TAL+  A
Sbjct: 197 ---GADVRLRALDGMSSLHAAAAHGHYSLVVWLVTFTDIGLTARDNEGATALHFAA 247

BLAST of MS013317 vs. ExPASy TrEMBL
Match: A0A6J1IIM4 (uncharacterized protein LOC111474320 OS=Cucurbita maxima OX=3661 GN=LOC111474320 PE=4 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.5e-247
Identity = 464/723 (64.18%), Postives = 542/723 (74.97%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WKN+ +E L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSLFDVMRGLYKAVIKEEWKNVEEE-LKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA S V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETP+FRA R
Sbjct: 61  LAAISTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPMFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IVE+ILEDC+DY SRSP NWT     PIIHA IQS+KFDVVL+L +FDKSLLEMK
Sbjct: 121 CGHLDIVEFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVVLKLAEFDKSLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL F SGSS+   E  IY +LP+EDIY Y    F SS    S+S  
Sbjct: 181 DSEGKTALQVLANMPLAFHSGSSLTFFESFIYTLLPNEDIYNYKPSNFESSKNHASQS-- 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YFV  LT  FWRFIFL               
Sbjct: 241 -LENKKNGDLEAGTNSNGTPNF-----WIYFVGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQT-EEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G  +  E   L I +IQ+   +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -STGISRSDENEKLDILQIQSIPINNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T   RN+LHVAIAHR+  I DWI K  L+MARLV RID  GFTALHH GIT
Sbjct: 421 ANPQAVDYLTVHDRNILHVAIAHRRKNIFDWILKRRLIMARLVNRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+ + TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQSQIPALYTMHHSKMKWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNSKTGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSKTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F M DFLH LPLKL  GFQLLFLSVA+TMMAFALTIVLTMK+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKMDDFLHTLPLKLSMGFQLLFLSVATTMMAFALTIVLTMKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYV+LVK+IW +RHNL +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVQLVKNIWGYRHNLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. ExPASy TrEMBL
Match: A0A6J1IIE1 (uncharacterized protein LOC111473443 OS=Cucurbita maxima OX=3661 GN=LOC111473443 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 9.9e-247
Identity = 463/723 (64.04%), Postives = 541/723 (74.83%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WKN+ +E L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSLFDVMRGLYKAVIKEEWKNVEEE-LKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA S V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETP+FRA R
Sbjct: 61  LAAISTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPMFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IVE+ILEDC+DY SRSP NWT     PIIHA IQS+KFDVVL+L +FDKSLLEMK
Sbjct: 121 CGHLDIVEFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVVLKLAEFDKSLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL F SGSS+   E  IY +LP+EDIY Y    F SS    S+S  
Sbjct: 181 DSEGKTALQVLANMPLAFHSGSSLTFFESFIYTLLPNEDIYNYKPSNFESSKNHASQS-- 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YFV  LT  FWRFIFL               
Sbjct: 241 -LENKKNGDLEAGTNSNGTPNF-----WIYFVGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQT-EEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G  +  E   L I +IQ+   +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -STGISRSDENEKLDILQIQSIPINNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T   RN+LHVAIAHR+  I DWI K  L+MARLV RID  GFTALHH GIT
Sbjct: 421 ANPLAVDYLTVHDRNILHVAIAHRRKNIFDWILKRRLIMARLVNRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+   TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQSQIPALYTMHHSKMRWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNSKTGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSKTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F + DFLH LPLKL  GFQLLFLSVA+TMMAFALTIVLTMK+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKIDDFLHTLPLKLSMGFQLLFLSVATTMMAFALTIVLTMKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYV+LVK+IW +RHNL +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVQLVKNIWGYRHNLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. ExPASy TrEMBL
Match: A0A6J1FII8 (uncharacterized protein LOC111446049 OS=Cucurbita moschata OX=3662 GN=LOC111446049 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.8e-240
Identity = 455/723 (62.93%), Postives = 539/723 (74.55%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           M   D MR  Y+  IK +WK++ QE L  + KI+FPMTT  DTA+HLAVYS +E+P KSL
Sbjct: 1   MSPFDVMRGLYKAVIKEEWKSV-QEELKEEMKIVFPMTTSKDTALHLAVYSGEEQPLKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAA + V  +FW NSAGNTPLHEAA +GNLAAVKLLV+Y K+DLLA+NIYGETPLFRA R
Sbjct: 61  LAAITTVRDDFWRNSAGNTPLHEAATIGNLAAVKLLVDYNKDDLLAENIYGETPLFRAAR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
            GHL IV++ILEDC+DY SRSP NWT     PIIHA IQS+KFDV L+L +FD+SLLEMK
Sbjct: 121 CGHLDIVKFILEDCDDYLSRSPRNWTTRNNKPIIHAVIQSQKFDVALKLAEFDESLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           D +GKTAL VLANMPL FRSGSS    E  IY +LPSEDIY Y      +S    + +S 
Sbjct: 181 DSEGKTALQVLANMPLAFRSGSSPTFFESFIYTLLPSEDIYHYK---PSNSESSKNHASC 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
            L NKKN DLEA  N +   NF     W YF   LT  FWRFIFL               
Sbjct: 241 CLKNKKNGDLEAGTNSNRPPNF-----WIYFGGCLTGLFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWK++Y+KK+HHKL VTIT  LA+ID SWR T  TP+  EID  
Sbjct: 301 -------------GWPQWKEMYKKKRHHKLGVTITNKLAQIDNSWRKTKVTPDTTEID-- 360

Query: 361 ARRGERKQIEEGSLLINKIQTEE-DDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
              G     E   L I +IQ++  +++ +  D +Y+D HETPLLLAAANGIIEIV+ I  
Sbjct: 361 -SIGISLSDENEKLDILEIQSKPVNNQGEIPDFEYNDHHETPLLLAAANGIIEIVDEIFQ 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           A+P AVDY+T + RN+LHVAIAHR+  I DWI K  L+MARLV+RID  GFTALHH GIT
Sbjct: 421 ANPQAVDYLTVQDRNILHVAIAHRRKNIFDWILKRRLIMARLVSRIDDMGFTALHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF  GG++GPALQLQEELKW+ RVQ QIP LY  HHS+ + TAR+ F+  H K+LEDGKE
Sbjct: 481 KFYSGGTHGPALQLQEELKWYERVQTQIPALYTMHHSKMKWTAREFFEKTHNKLLEDGKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           WLKKTSESCSAVAVLI+TVVFAAAYTVPGGLNS TGSPVL+T+PIY+VFT+MDI+GLAT+
Sbjct: 541 WLKKTSESCSAVAVLISTVVFAAAYTVPGGLNSNTGSPVLLTEPIYIVFTIMDIIGLATA 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           LTS+VLFLS+LTS F M  FL+ LPLKL  GFQLLFLSVA+TMMAFALTIVLT+K+ E+K
Sbjct: 601 LTSLVLFLSVLTSSFKMDAFLYTLPLKLSMGFQLLFLSVATTMMAFALTIVLTVKTEEMK 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           WT+SLLY+A+FFPVTMFII+Q+PLYVELVK+IW +RH+L +F P+GFV  FW  PSKI+T
Sbjct: 661 WTVSLLYLATFFPVTMFIIIQMPLYVELVKNIWGYRHSLSKFFPMGFVALFWNAPSKILT 683

Query: 721 RKF 723
           RKF
Sbjct: 721 RKF 683

BLAST of MS013317 vs. ExPASy TrEMBL
Match: A0A6J1CLX1 (uncharacterized protein LOC111012667 OS=Momordica charantia OX=3673 GN=LOC111012667 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 8.2e-225
Identity = 397/426 (93.19%), Postives = 398/426 (93.43%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL
Sbjct: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60

Query: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120
           LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR
Sbjct: 61  LAATSEVEREFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAVR 120

Query: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180
           FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK
Sbjct: 121 FGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEMK 180

Query: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240
           DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG
Sbjct: 181 DEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESSG 240

Query: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALLC 300
           ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFL               
Sbjct: 241 ALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFL--------------- 300

Query: 301 SSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360
                        GWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG
Sbjct: 301 -------------GWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDYG 360

Query: 361 ARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVDA 420
           ARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVDA
Sbjct: 361 ARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVDA 398

Query: 421 HPPAVD 427
           HPPA+D
Sbjct: 421 HPPAID 398

BLAST of MS013317 vs. ExPASy TrEMBL
Match: A0A6J1JXD5 (uncharacterized protein LOC111490543 OS=Cucurbita maxima OX=3661 GN=LOC111490543 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 2.1e-180
Identity = 363/722 (50.28%), Postives = 478/722 (66.20%), Query Frame = 0

Query: 1   MDFLDEMRSAYRTAIKGKWKNLPQEILIHKAKIMFPMTTGGDTAMHLAVYSRKEEPTKSL 60
           MD   E ++ Y+ A++ +W+ +  E+     + + P+  GGDTA+HLAV+S KEEP K+ 
Sbjct: 1   MDLYKEKKALYKFALREQWEYV-GEVCKDARQFVLPINNGGDTALHLAVHSGKEEPLKTF 60

Query: 61  LAATSEVE-REFWTNSAGNTPLHEAAAVGNLAAVKLLVEYKKEDLLAQNIYGETPLFRAV 120
           LA   E+E  E W +   NTPLHEAA+VGNL AVKLLVE +KEDLL  NI+GETPL+ A 
Sbjct: 61  LAQVMEMEYTEHWKSYVENTPLHEAASVGNLGAVKLLVELRKEDLLEVNIWGETPLYSAA 120

Query: 121 RFGHLHIVEYILEDCEDYFSRSPINWTKEKEFPIIHAAIQSEKFDVVLRLVDFDKSLLEM 180
           R+G L IV+YIL DCED+++RS +NWT  K  PIIHAAIQSE F++V+ LVDFDKSLLEM
Sbjct: 121 RYGKLDIVQYILNDCEDFYARSSLNWT-AKGTPIIHAAIQSENFEMVMMLVDFDKSLLEM 180

Query: 181 KDEDGKTALYVLANMPLIFRSGSSMGSLEKIIYAVLPSEDIYKYNFKTFGSSNKDDSESS 240
           KD + KTAL+VLANMP IF SG   G   ++IY  LP +  Y +NF  FG  N +DS +S
Sbjct: 181 KDTNNKTALHVLANMPHIFESGYVKGFWAELIYYWLPKDPTYDFNFCKFGYVNNNDS-TS 240

Query: 241 GALNNKKNEDLEAQHNPDYGLNFCRSNCWKYFVHFLTCYFWRFIFLGFTLSLLSLSSALL 300
            +  +KK EDLEA      G +    + W        C+ W           L  SS  L
Sbjct: 241 LSNKDKKFEDLEA------GFDSKPKHNW--------CFKWS--------KYLGQSSKRL 300

Query: 301 CSSLILILNCETCIGWPQWKQLYRKKQHHKLAVTITKMLAEIDYSWRNTDSTPEINEIDY 360
               I+        GW + K+LY KK+ HKL + IT+ML +ID S       P+      
Sbjct: 301 LGRFIV-------RGWLECKELYMKKKKHKLILRITEMLVDIDLSCYQEKQKPD------ 360

Query: 361 GARRGERKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVD 420
                        S LI++ +  ++     K I+Y D HETPLLLAAA GIIE+VE I++
Sbjct: 361 -------------STLIDRSEKAKEPIVDGKSIEYKDHHETPLLLAAARGIIEVVERIIE 420

Query: 421 AHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAGIT 480
           AHP AVDYVT+K RN+LHV IAHRQ +I +WI++++L+M RL  RID  G+T LHH GIT
Sbjct: 421 AHPEAVDYVTEKDRNILHVLIAHRQLKIFEWIQRQKLIMHRLTRRIDVLGYTVLHHVGIT 480

Query: 481 KFSRGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKE 540
           KF    + GPA+QLQ EL+WF RV + +P LY   +S+N+   R+ FD  H+ ML+  KE
Sbjct: 481 KFLSQSTLGPAIQLQHELEWFDRVHNVLPLLYNMQYSKNQWNPREFFDETHKDMLDKAKE 540

Query: 541 WLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATS 600
           W+KKTSESCSAVAVL+ATV FAAA+TVPGGLNSKTGSP+L++DPIY+ F  +DI  L +S
Sbjct: 541 WIKKTSESCSAVAVLMATVAFAAAFTVPGGLNSKTGSPILLSDPIYLFFAALDITSLISS 600

Query: 601 LTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIK 660
           L+S+VLFL ILTSPF +  F H L ++L  GF LLFLSVASTM+AFA+ IVLTMKS  + 
Sbjct: 601 LSSLVLFLLILTSPFEIDTFRHELTIQLSLGFNLLFLSVASTMIAFAVAIVLTMKSTNMV 660

Query: 661 WTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSFRHNLFQFLPIGFVTPFWMLPSKIVT 720
           W   LL++ +  P+ +F++M+LPL +EL KSIW     L++ LP+GF+T F+ +PSK ++
Sbjct: 661 WAECLLFLVTLAPIVIFVVMKLPLLLELEKSIWKGLQFLWKLLPMGFLTIFFEIPSKFLS 671

Query: 721 RK 722
            K
Sbjct: 721 NK 671

BLAST of MS013317 vs. TAIR 10
Match: AT3G18670.1 (Ankyrin repeat family protein )

HSP 1 Score: 144.1 bits (362), Expect = 4.5e-34
Identity = 96/294 (32.65%), Postives = 162/294 (55.10%), Query Frame = 0

Query: 402 LLLAAANGIIEIVEAIVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARL 461
           L  A  NGI+E +E ++  +P  V        N+   A++ RQ +I   I         L
Sbjct: 293 LFKAVENGIVEYIEEMMRHYPDIVWSKNSSGLNIFFYAVSQRQEKIFSLIYNIGAKKNIL 352

Query: 462 VTRIDASGFTALHHAGI-TKFSRGGSY-GPALQLQEELKWFHRVQDQIPTLYAKH-HSQN 521
            T  D      LHHA      SR     G ALQ+Q EL+WF  V+  +   + K  + + 
Sbjct: 353 ATNWDIFHNNMLHHAAYRAPASRLNLIPGAALQMQRELQWFKEVEKLVQPKHRKMVNLKQ 412

Query: 522 EGTARQHFDNQHQKMLEDGKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPV 581
           + T +  F +QH+ ++E G++W+K+T+ SC+ VA LI T++F++A+TVPGG  S  G P+
Sbjct: 413 KKTPKALFTDQHKDLVEQGEKWMKETATSCTVVAALITTMMFSSAFTVPGGYRS-DGMPL 472

Query: 582 LVTDPIYMVFTVMDIVGLATSLTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSV 641
            +    + +F + D + L TS  S+++FL IL S +   DFL +LP KL+ G   LFLS+
Sbjct: 473 YIHQHRFKIFLISDAISLFTSCMSLLMFLGILKSRYREEDFLRSLPTKLIVGLLALFLSM 532

Query: 642 ASTMMAFALTIVLTMKSAEIKWTMSLLYMASFFPVTMFIIMQLPLYVELVKSIW 693
           A+ ++ F +T+ +T+   +I W  +     +  P+ MF+++Q P+ +E+ ++ +
Sbjct: 533 ATMIVTFVVTL-MTLVGEKISWVSAQFMFLAVIPLGMFVVLQFPVLLEIFRATY 584

BLAST of MS013317 vs. TAIR 10
Match: AT5G04730.1 (Ankyrin-repeat containing protein )

HSP 1 Score: 134.8 bits (338), Expect = 2.8e-31
Identity = 102/337 (30.27%), Postives = 178/337 (52.82%), Query Frame = 0

Query: 366 RKQIEEGSLLINKIQTEEDDKSKSKDIDYDDIHETPLLLAAANGIIEIVEAIVDAHPPAV 425
           +K+  +   L+ ++ T   D     +I + +     LL AA +G  +    I+  +   +
Sbjct: 262 KKRHSQAQKLLKQMCTSLRDIMAKNEIRWKETVYEALLEAAKSGNRDFFIEIIKCNSQLL 321

Query: 426 DYVTKKQ-RNVLHVAIAHRQSQILDWIEKEELVMARLVTRIDASGFTALHHAG--ITKFS 485
             +     RN+  +A+  ++ +I + I   +     L+   D      LH AG   T   
Sbjct: 322 WILNPTSGRNLFQLAVEFKKEKIFNLIHGLDDRKVTLLRSYDKGNNNILHIAGRLSTPDQ 381

Query: 486 RGGSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKEWLK 545
                G AL++Q E +WF  V+  +        +++  T RQ F++ H+ + ++G+EW+K
Sbjct: 382 LSKISGAALKMQRESQWFKEVESLVSEREVVQKNKDNKTPRQIFEHYHEHLRKEGEEWMK 441

Query: 546 KTSESCSAVAVLIATVVFAAAYTVPGGLNSKTGSPVLVTDPIYMVFTVMDIVGLATSLTS 605
            T+ +CS VA LIATV F A +TVPGG++  +GSP+++ D  +  F   D +    S  S
Sbjct: 442 YTATACSFVAALIATVTFQAIFTVPGGIDGTSGSPLILNDLHFRAFIFTDTLAFFASCIS 501

Query: 606 VVLFLSILTSPFMMHDFLHALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIKWTM 665
           V++FLSILTS +   DF+ +LP K++ G  +LF+S+AS ++AF     +T  SA ++   
Sbjct: 502 VLIFLSILTSRYSFDDFIVSLPRKMILGQSILFISIASMLVAF-----ITSLSASMRHKP 561

Query: 666 SLLY----MASFFPVTMFIIMQLPLYVELVKSIWSFR 696
           +L+Y    +AS FP  +F+++Q PL  E++ S +  R
Sbjct: 562 ALVYPLKPLAS-FPSLLFLMLQYPLLKEMISSTYGKR 592

BLAST of MS013317 vs. TAIR 10
Match: AT5G35810.1 (Ankyrin repeat family protein )

HSP 1 Score: 134.0 bits (336), Expect = 4.7e-31
Identity = 93/306 (30.39%), Postives = 171/306 (55.88%), Query Frame = 0

Query: 397 IHETPLLL--AAANGIIEIVEAIVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKE 456
           +  +P+LL  AA +G +E++  ++ ++P  +  V  K +++ H+A  +R  +I + I + 
Sbjct: 28  VGSSPMLLFDAAQSGNLELLLILIRSYPDLIWTVDHKNQSLFHIAAINRHEKIFNRIYEL 87

Query: 457 ELVMARLVTRIDASGFTALHH--AGITKFSR-GGSYGPALQLQEELKWFHRVQDQIPTLY 516
             +   +    +      L H  A +   +R     G ALQ+Q E+ W+  V++ +P +Y
Sbjct: 88  GAIKDLIAMYKEKESNDNLLHLVARLPPPNRLQVVSGAALQMQREILWYKAVKEIVPRVY 147

Query: 517 AKHHSQNEGTARQHFDNQHQKMLEDGKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLN 576
            K  ++ E  A   F  +H  + ++G++W+K+T+ +C  V+ LIATVVFAAA+T+PGG +
Sbjct: 148 IKTKNKKEEVAHDLFTKEHDNLRKEGEKWMKETATACILVSTLIATVVFAAAFTLPGGND 207

Query: 577 SK-----TGSPVLVTDPIYMVFTVMDIVGLATSLTSVVLFLSILTSPFMMHDFLHALPLK 636
           +       G P    +  + VF + D V L +S+TS+++FLSILTS +    F   LP K
Sbjct: 208 TSGDIKTLGFPTFRKEFWFEVFIISDSVALLSSVTSIMIFLSILTSRYAEASFQTTLPTK 267

Query: 637 LLFGFQLLFLSVASTMMAFALTIVLTMKSAEIKWTMSLLYMASFFPVTMFIIMQLPLYVE 693
           L+ G   LF+S+ S ++AF  T++L ++  E KW++ LL   +      F+++   L+ +
Sbjct: 268 LMLGLLALFVSIISMVLAFTATLIL-IRDQEPKWSLILLVYVASATALSFVVLHFQLWFD 327

BLAST of MS013317 vs. TAIR 10
Match: AT5G04700.1 (Ankyrin repeat family protein )

HSP 1 Score: 131.0 bits (328), Expect = 4.0e-30
Identity = 100/312 (32.05%), Postives = 162/312 (51.92%), Query Frame = 0

Query: 386 KSKSKDIDYDDIHETPLLLAAANGIIE-IVEAIVDAHPPAVDYVTKKQRNVLHVAIAHRQ 445
           K +S+ +D        LL A   G ++ +VE I +         T     +  +A+  RQ
Sbjct: 353 KERSETVD------EALLFAVRYGNVDFLVEMIRNNSELLWSTRTSSSSTLFLLAVEFRQ 412

Query: 446 SQILDWIEKEELVMARLVTRIDASGFTALHHAGITK--FSRGGSYGPALQLQEELKWFHR 505
            ++   +   +     L+   D  G   LH AG            G  LQLQ EL+WF  
Sbjct: 413 EKVFSLLYGLDDRKYLLLADKDCDGNGVLHLAGFPSPPSKLSSVVGAPLQLQRELQWFKE 472

Query: 506 VQDQIPTLYAKHHSQNEGTARQHFDNQHQKMLEDGKEWLKKTSESCSAVAVLIATVVFAA 565
           V+   P +  +  +  E T  + F  +HQ + ++ ++W+K T+ SCS VA LI TV FAA
Sbjct: 473 VERIAPEIEKERVNTEEQTPIEIFTKEHQGLRQEAEKWMKDTAMSCSLVAALIVTVTFAA 532

Query: 566 AYTVPGGL--NSKTGSPVLVTDPIYMVFTVMDIVGLATSLTSVVLFLSILTSPFMMHDFL 625
            +TVPGG   NSK G P  + D  +++F V D++    S TSV++FL ILT+ +   DFL
Sbjct: 533 VFTVPGGTDDNSK-GKPFHLRDRRFIIFIVSDLISCFASCTSVLIFLGILTARYSFDDFL 592

Query: 626 HALPLKLLFGFQLLFLSVASTMMAFALTIVLTMKSAEIKWTMSLLYMASFFPVTMFIIMQ 685
             LP K++ G  +LF+S+A+ ++AF+ + + TM   E KW ++   + +  P  +F+++Q
Sbjct: 593 VFLPTKMIAGLSILFVSIAAMLIAFS-SALFTMMGKEGKWIVAPTILFACLPALLFVLLQ 652

Query: 686 LPLYVELVKSIW 693
            PL  E++ S +
Sbjct: 653 YPLLKEMIFSTY 656

BLAST of MS013317 vs. TAIR 10
Match: AT3G54070.1 (Ankyrin repeat family protein )

HSP 1 Score: 116.7 bits (291), Expect = 7.8e-26
Identity = 93/308 (30.19%), Postives = 160/308 (51.95%), Query Frame = 0

Query: 405 AAANGIIEIVEAIVDAHPPAVDYVTKKQRNVLHVAIAHRQSQILDWIEKEELVMARLVTR 464
           AA  G +EI+  ++ +H   +  V    R + HVA  +R   I   I +   +   + + 
Sbjct: 262 AAELGNVEILVILIRSHLDLLWIVDNNNRTLFHVAALYRHENIFSLIYELGGIKDLIASY 321

Query: 465 IDASGFTALHH--AGITKFSRG--GSYGPALQLQEELKWFHRVQDQIPTLYAKHHSQNEG 524
            +      L H  A +   +R   GS G AL +Q+EL WF  V++ +P  Y +  +    
Sbjct: 322 KEKQSKDTLLHLVARLPPMNRQQVGS-GAALHMQKELLWFKAVKEIVPRSYIETKNTKGE 381

Query: 525 TARQHFDNQHQKMLEDGKEWLKKTSESCSAVAVLIATVVFAAAYTVPGGLN------SKT 584
            A   F  QH+ + ++G+ W+K+T+ +C   A LIATVVFAAA T+PGG +      +  
Sbjct: 382 LAHDIFTEQHENLRKEGERWMKETATACMLGATLIATVVFAAAITIPGGNDDSGDKANTL 441

Query: 585 GSPVLVTDPIYMVFTVMDIVGLATSLTSVVLFLSILTSPFMMHDFLHALPLKLLFGFQLL 644
           G P      ++ +FT+ D V L +S+ S+V+FLSI TS +   DF + LP KL+FG   L
Sbjct: 442 GFPNFRKRLLFDIFTLSDSVALFSSMMSIVIFLSIFTSRYAEEDFRYDLPTKLMFGLSAL 501

Query: 645 FLSVASTMMAFALTIVLTMKSAEIKWTMSLLYMASFFPVTMFIIMQLPLYVELVKSIWSF 703
           F+S+ S ++AF  +++L ++  +   ++ L+   +      F  +   L+   ++S++  
Sbjct: 502 FISIISMILAFTFSMIL-IRVEKASLSLVLISCLASLTALTFAYLYFHLWFNTLRSVYI- 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894049.17.3e-25264.28uncharacterized protein LOC120082801 isoform X1 [Benincasa hispida][more]
XP_022975228.13.2e-24764.18uncharacterized protein LOC111474320 [Cucurbita maxima][more]
XP_022974704.12.1e-24664.04uncharacterized protein LOC111473443 [Cucurbita maxima][more]
XP_031739511.17.6e-24161.85uncharacterized protein LOC101203890 [Cucumis sativus][more]
XP_022940456.13.7e-24062.93uncharacterized protein LOC111446049 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9ET476.2e-1233.97Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2[more]
Q636189.0e-1133.97Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2[more]
B1AK531.5e-1034.75Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1[more]
C7B1781.9e-0828.49Protein VAPYRIN OS=Petunia hybrida OX=4102 GN=VPY PE=2 SV=1[more]
Q3UYR44.2e-0830.17Espin-like protein OS=Mus musculus OX=10090 GN=Espnl PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IIM41.5e-24764.18uncharacterized protein LOC111474320 OS=Cucurbita maxima OX=3661 GN=LOC111474320... [more]
A0A6J1IIE19.9e-24764.04uncharacterized protein LOC111473443 OS=Cucurbita maxima OX=3661 GN=LOC111473443... [more]
A0A6J1FII81.8e-24062.93uncharacterized protein LOC111446049 OS=Cucurbita moschata OX=3662 GN=LOC1114460... [more]
A0A6J1CLX18.2e-22593.19uncharacterized protein LOC111012667 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A6J1JXD52.1e-18050.28uncharacterized protein LOC111490543 OS=Cucurbita maxima OX=3661 GN=LOC111490543... [more]
Match NameE-valueIdentityDescription
AT3G18670.14.5e-3432.65Ankyrin repeat family protein [more]
AT5G04730.12.8e-3130.27Ankyrin-repeat containing protein [more]
AT5G35810.14.7e-3130.39Ankyrin repeat family protein [more]
AT5G04700.14.0e-3032.05Ankyrin repeat family protein [more]
AT3G54070.17.8e-2630.19Ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 110..139
e-value: 0.15
score: 21.2
coord: 397..427
e-value: 110.0
score: 11.7
coord: 40..69
e-value: 160.0
score: 10.9
coord: 76..106
e-value: 0.0055
score: 25.9
coord: 149..178
e-value: 1500.0
score: 3.7
coord: 431..460
e-value: 3500.0
score: 1.0
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 110..142
score: 8.522659
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 76..98
score: 9.85816
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 11..197
e-value: 1.6E-28
score: 101.5
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 338..547
e-value: 1.1E-11
score: 46.8
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 35..190
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 395..476
IPR026961PGG domainPFAMPF13962PGGcoord: 538..651
e-value: 5.9E-24
score: 84.1
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 45..133
e-value: 6.1E-12
score: 46.0
NoneNo IPR availablePANTHERPTHR24177:SF331REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 22..634
NoneNo IPR availablePANTHERPTHR24177CASKINcoord: 22..634
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 76..98
score: 9.834415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013317.1MS013317.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding