MS013310 (gene) Bitter gourd (TR) v1

Overview
NameMS013310
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontropinone reductase-like 3
Locationscaffold402: 224672 .. 227333 (-)
RNA-Seq ExpressionMS013310
SyntenyMS013310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTATACGAAGATCGGGAAAAGGTTTCAGGGGAAGGTGGCAATTGTCACTGCTTCAACTCAGGGTATCGGCTTCCAAATCGCCGAACGTCTCGCCGTGGAAGGTGCCTCCGTTGTTATATCTTCCCGCAGACAGGTTCATCGATGTTCCCCAATTCTTGTTTTAATCTCAACAAATTTGAAATTATCGCTTGATTTTGCTGTTTTCAATTTCCGGTGTGATTCTTGCTTCTGGTTTGTCACTGTGAGATTTCAGGATTGAATGTCTTGAAATTAGATCCAGATCAATTCTCGAACTCATTGTTTCTCAATTGTTATGTTTTTCTCTTGAATTTTGTATCACTGTTTATTTGGCCTGTTGCCGTTTAATTTCTATAGTGAAGGCATGCCATGGATTTGTGCTTTAAAATACACACCTTCCGAGATGAAAACAGATTTTGTGAAAGTTCAAATATAAAGACAGAACATCTCAAAACATACAGATGAAACTGGAATAAGTTTGAAAGTTCAAAGAATCTAAACTGGATTATTTATTTATATCTTTTTTTGCTGTCAAATTTGAGATGATTCAATACTTGTATCTTCACTTAAAAGTCCACCTTATAAGCCCGGGTCGAAAAGAAAAGAAAAAGAGAATATTGGTATGGATTAGTATTTAGTCCAACGAGGATATATTAGAGAAAAGAATCGACTCCAGAAAGAAAATCTTATATAACTATCACTTCAATATCAACTTGGCTAATTGTAGATGAATAATTAAGTTGAAATTTAAATAACTGGTTCTAATTTATAAAATTTTGAATACTTAAATGGTCATCATCAGTAAATATTTTTCTACCCATTATCAGTGTGAAGAATAAATCAGAGGTGTTATAAAAAGTATTTCATGAATGTTATGCATACTTTCTTTCTTAATCATATACCAGTTGGCCTCTAGTCCACCCTCCCTGAAACATTTAAACGAACAAAGAAAGGGAGAAAGAAAAAGAAAAGAGTGGAAAAATAAATAAAAACCTGGTCTTCTATCTAGAAATTTGAGTTTGATGTGTTTTATGTTGTTTCAAAGTATTTTTTCCAAAAATATTCTTTCTATTCATTCAGAGTTCCTCCGTTACCTACTAGAAGGAATTCACCTTTCGTGTTTTATTTGTATGTTTCACAACCTCCTTTTAACTCTAGTTAGCGTTATTGTAGAAAAATGTAGATGAGGCAGTTGAAAAACTCAAAGCTCGAGGACTTGAAGTGTTCGGGGTTGTTTGTCATGTGTCAAATGCACAGCAAAGGAAGAACCTTGTAGAAAAGACTATTCAGGTGAGACTATTTACTTCCTTCTTTTTAAGTTCTTCTGAACTGAGTGAGAACTGGAAGTGGTTATAATAGATGATTACTCAACATTATTTTAAAAACCATCATGTTACTGGAAGATTCGAGCTTATAAGTGCATATGTTTTTGTTTCTGAAGGCAAACAAGTTCCACATTCCTACAACTAAAAAAATGGGCAGATTTTCTTTATAATCTTTGTATTCTTGGTGATAATCATGGATAGAGTTATATCAAGCTCAGAGTTAATGATATACATACTATTTCTGTTTTCTCTTTCCTTTGCATATATCAATGAGGTTGGTTATGATATTTTAAAACATGAGAGAGAGAGAGGGAGAGAGCGTTAGAGAGTTCCATCAGGTGAGGACTTTATTCATGTTACCTTGTAGCTTGTAATTATCCACCAATCCTTGAAGGTTGTTACATCAAATAATTAAATTTCAATGGTTTTCTTTCAGAAATATGGGAAAATAGATGTAGTTGTATCAAATGCAGCTGTCAATCCATCAGTAGATTCCATTTTGAAAACTCAAGAATCTGTCCTTGACAAGCTGTGGGACATAAATGTTAAAGCTTCGGTCCTACTTTTGCAGGTATTATACTGTACTGATGCATAATATTTCATGATTCAGGGCTTTTTGGAATTAAAGATTATGCTGATTTGAGATTTGATTATTTGTGAACTTCAAAAATCTTTTGAACTACCCCCTTCAATATGAACACTACTTGTGCATATATTTAAAATTTCATTAATTTCTGGTACAGGAAGTAGCTCCTCACTTGCAGAAAGGCTCTTCAGTTGTTCTCATTTCCTCCATTGAAGGATACCAGCCTTCTGCCTTGTTGGCCATGTATGGGGTGACAAAAACAGCTCTTCTTGGACTGACTAAGGTTAAACAATGGGTTTACATGCTTTTTGCAACTATTGTGACTTCTGAAAATTAAATATTTATTTTTTCTTTTGCTTGTTTTGTTGATTTCCTTCAGGCTCTCGCAGCAGAGATGGCCCCTGACACTCGTGTAAACTGTGTCGCCCCTGGTTTCGTACCGACGCACTTTGCTGATTTCCTCACGTCTAATGATGCCATTGTAAGTAACCCATTTCAATGGATTGGCTTTCTTATTGCACTGGTAAATACTAAATAGCAAAGTTTGTTACTTACTACACTTGGATTTGAACCAAATTCAGAAGAAGGGCGTTGAGGCAAAGACCTTACTCAATCGGCTTGGTACGACCGAAGACATGGCTGCAGCCACTGCCTTCCTGGCATCGGACGACGCTTCATATATCACAGGTGAAACTCTAGTGGTGGCTGGGGGGATGTCTTCCAGACTC

mRNA sequence

ATGGATTATACGAAGATCGGGAAAAGGTTTCAGGGGAAGGTGGCAATTGTCACTGCTTCAACTCAGGGTATCGGCTTCCAAATCGCCGAACGTCTCGCCGTGGAAGGTGCCTCCGTTGTTATATCTTCCCGCAGACAGAAAAATGTAGATGAGGCAGTTGAAAAACTCAAAGCTCGAGGACTTGAAGTGTTCGGGGTTGTTTGTCATGTGTCAAATGCACAGCAAAGGAAGAACCTTGTAGAAAAGACTATTCAGAAATATGGGAAAATAGATGTAGTTGTATCAAATGCAGCTGTCAATCCATCAGTAGATTCCATTTTGAAAACTCAAGAATCTGTCCTTGACAAGCTGTGGGACATAAATGTTAAAGCTTCGGTCCTACTTTTGCAGGAAGTAGCTCCTCACTTGCAGAAAGGCTCTTCAGTTGTTCTCATTTCCTCCATTGAAGGATACCAGCCTTCTGCCTTGTTGGCCATGTATGGGGTGACAAAAACAGCTCTTCTTGGACTGACTAAGGCTCTCGCAGCAGAGATGGCCCCTGACACTCGTGTAAACTGTGTCGCCCCTGGTTTCGTACCGACGCACTTTGCTGATTTCCTCACGTCTAATGATGCCATTAAGAAGGGCGTTGAGGCAAAGACCTTACTCAATCGGCTTGGTACGACCGAAGACATGGCTGCAGCCACTGCCTTCCTGGCATCGGACGACGCTTCATATATCACAGGTGAAACTCTAGTGGTGGCTGGGGGGATGTCTTCCAGACTC

Coding sequence (CDS)

ATGGATTATACGAAGATCGGGAAAAGGTTTCAGGGGAAGGTGGCAATTGTCACTGCTTCAACTCAGGGTATCGGCTTCCAAATCGCCGAACGTCTCGCCGTGGAAGGTGCCTCCGTTGTTATATCTTCCCGCAGACAGAAAAATGTAGATGAGGCAGTTGAAAAACTCAAAGCTCGAGGACTTGAAGTGTTCGGGGTTGTTTGTCATGTGTCAAATGCACAGCAAAGGAAGAACCTTGTAGAAAAGACTATTCAGAAATATGGGAAAATAGATGTAGTTGTATCAAATGCAGCTGTCAATCCATCAGTAGATTCCATTTTGAAAACTCAAGAATCTGTCCTTGACAAGCTGTGGGACATAAATGTTAAAGCTTCGGTCCTACTTTTGCAGGAAGTAGCTCCTCACTTGCAGAAAGGCTCTTCAGTTGTTCTCATTTCCTCCATTGAAGGATACCAGCCTTCTGCCTTGTTGGCCATGTATGGGGTGACAAAAACAGCTCTTCTTGGACTGACTAAGGCTCTCGCAGCAGAGATGGCCCCTGACACTCGTGTAAACTGTGTCGCCCCTGGTTTCGTACCGACGCACTTTGCTGATTTCCTCACGTCTAATGATGCCATTAAGAAGGGCGTTGAGGCAAAGACCTTACTCAATCGGCTTGGTACGACCGAAGACATGGCTGCAGCCACTGCCTTCCTGGCATCGGACGACGCTTCATATATCACAGGTGAAACTCTAGTGGTGGCTGGGGGGATGTCTTCCAGACTC

Protein sequence

MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLVVAGGMSSRL
Homology
BLAST of MS013310 vs. NCBI nr
Match: XP_022142483.1 (tropinone reductase-like 3 [Momordica charantia])

HSP 1 Score: 475.7 bits (1223), Expect = 2.5e-130
Identity = 254/255 (99.61%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG
Sbjct: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV GVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI
Sbjct: 61  LEVSGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGMSSRL
Sbjct: 241 TGETLVVAGGMSSRL 255

BLAST of MS013310 vs. NCBI nr
Match: XP_008458979.1 (PREDICTED: tropinone reductase-like 3 [Cucumis melo])

HSP 1 Score: 449.9 bits (1156), Expect = 1.5e-122
Identity = 237/255 (92.94%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD  KIGKRF+GKVAIVTASTQGIGFQIA RLA EGASVVISSRRQKNVDEAVEKLKA G
Sbjct: 1   MDNGKIGKRFRGKVAIVTASTQGIGFQIARRLAFEGASVVISSRRQKNVDEAVEKLKAEG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI
Sbjct: 61  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQ+GSSVVLISSIEGYQPS+LLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQDVAPHLQRGSSVVLISSIEGYQPSSLLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFL +N+A++KGVE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 DTRVNCVAPGFVPTHFADFLVTNEAVRKGVESKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 241 TGETLVVAGGMPSRL 255

BLAST of MS013310 vs. NCBI nr
Match: KAG6573495.1 (hypothetical protein SDJN03_27382, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 446.8 bits (1148), Expect = 1.2e-121
Identity = 236/255 (92.55%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD TK+ KRFQGKVAIVTASTQGIGF+IA RLA+EGASVVISSRRQ+NVDEAVEKLKA G
Sbjct: 20  MDNTKMAKRFQGKVAIVTASTQGIGFRIAHRLALEGASVVISSRRQRNVDEAVEKLKAEG 79

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSIL+TQE+VLDKLWDI
Sbjct: 80  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILRTQEAVLDKLWDI 139

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQKGSSVVLISSIEG+QPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 140 NVKASVLLLQDVAPHLQKGSSVVLISSIEGFQPSALLAMYGVTKTALLGLTKALAAEMAP 199

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFL SN+AIKK VE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 200 DTRVNCVAPGFVPTHFADFLVSNEAIKKEVESKTLLNRLGTTEDMAAATAFLASDDASYI 259

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 260 TGETLVVAGGMPSRL 274

BLAST of MS013310 vs. NCBI nr
Match: XP_022925358.1 (tropinone reductase-like 3 [Cucurbita moschata] >XP_022925359.1 tropinone reductase-like 3 [Cucurbita moschata])

HSP 1 Score: 446.4 bits (1147), Expect = 1.6e-121
Identity = 235/255 (92.16%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD TK+ KRFQGKVAIVTASTQGIGF+IA RLA+EGASVVISSRRQ+NVDEAVEKLKA G
Sbjct: 20  MDNTKMAKRFQGKVAIVTASTQGIGFRIAHRLALEGASVVISSRRQRNVDEAVEKLKAEG 79

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSIL+TQE++LDKLWDI
Sbjct: 80  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILRTQEAILDKLWDI 139

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQKGSSVVLISSIEG+QPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 140 NVKASVLLLQDVAPHLQKGSSVVLISSIEGFQPSALLAMYGVTKTALLGLTKALAAEMAP 199

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFL SN+AIKK VE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 200 DTRVNCVAPGFVPTHFADFLVSNEAIKKEVESKTLLNRLGTTEDMAAATAFLASDDASYI 259

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 260 TGETLVVAGGMPSRL 274

BLAST of MS013310 vs. NCBI nr
Match: XP_023525934.1 (tropinone reductase-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445.3 bits (1144), Expect = 3.6e-121
Identity = 235/255 (92.16%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD TK+ KRFQGKVAIVTASTQGIGF+IA RLA+EGASVVISSRRQ+NVDEAVEKLKA G
Sbjct: 1   MDNTKMAKRFQGKVAIVTASTQGIGFRIAHRLALEGASVVISSRRQRNVDEAVEKLKAEG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSIL+TQE+VLDKLWDI
Sbjct: 61  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILRTQEAVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQKGSSVVLISSIEG+QPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQDVAPHLQKGSSVVLISSIEGFQPSALLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFA+FL SN+AIKK VE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 DTRVNCVAPGFVPTHFAEFLVSNEAIKKEVESKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 241 TGETLVVAGGMPSRL 255

BLAST of MS013310 vs. ExPASy Swiss-Prot
Match: H9BFQ2 (Tropinone reductase-like 3 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 1.5e-106
Identity = 199/250 (79.60%), Postives = 227/250 (90.80%), Query Frame = 0

Query: 6   IGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFG 65
           IG+RF+GKVAIVTASTQGIGF IA RL +EGA+VVISSR+QKNVDEAVEKLKA+G+EV G
Sbjct: 9   IGRRFEGKVAIVTASTQGIGFAIAYRLGLEGAAVVISSRKQKNVDEAVEKLKAQGIEVLG 68

Query: 66  VVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKAS 125
           VVCHVSN++QRKNL++ T++KYGKIDVVVSNAA NPS D++L+TQESVLDK+W+INVKAS
Sbjct: 69  VVCHVSNSKQRKNLIDTTVKKYGKIDVVVSNAAANPSTDALLETQESVLDKIWEINVKAS 128

Query: 126 VLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDTRVN 185
           +LLLQE APHLQKGSSVVLISSI GYQP A +AMYGVTKTALLGLTKALA EMAP  RVN
Sbjct: 129 ILLLQEAAPHLQKGSSVVLISSITGYQPPASMAMYGVTKTALLGLTKALAEEMAPYVRVN 188

Query: 186 CVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETL 245
           CVAPGFVPT+FAD+LT N+ I+  +E KT L RLGTT+DMA+ATAFLASDDASYITGET+
Sbjct: 189 CVAPGFVPTNFADYLTRNEDIRNSLEEKTFLKRLGTTQDMASATAFLASDDASYITGETV 248

Query: 246 VVAGGMSSRL 256
           VVAGG  SRL
Sbjct: 249 VVAGGTPSRL 258

BLAST of MS013310 vs. ExPASy Swiss-Prot
Match: Q9S9W2 (Short-chain dehydrogenase/reductase SDRA OS=Arabidopsis thaliana OX=3702 GN=SDRA PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 1.7e-102
Identity = 186/251 (74.10%), Postives = 221/251 (88.05%), Query Frame = 0

Query: 5   KIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVF 64
           K+ +R +GKVAIVTASTQGIGF I ER  +EGASVV+SSR+Q NVDEAV KLK++G++ +
Sbjct: 4   KLPRRLEGKVAIVTASTQGIGFGITERFGLEGASVVVSSRKQANVDEAVAKLKSKGIDAY 63

Query: 65  GVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKA 124
           G+VCHVSNAQ R+NLVEKT+QKYGKID+VV NAA NPS D IL ++E+VLDKLW+INVK+
Sbjct: 64  GIVCHVSNAQHRRNLVEKTVQKYGKIDIVVCNAAANPSTDPILSSKEAVLDKLWEINVKS 123

Query: 125 SVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDTRV 184
           S+LLLQ++APHL+KGSSV+ I+SI G+ P   +AMYGVTKTALLGLTKALAAEMAPDTRV
Sbjct: 124 SILLLQDMAPHLEKGSSVIFITSIAGFSPQGAMAMYGVTKTALLGLTKALAAEMAPDTRV 183

Query: 185 NCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGET 244
           N VAPGFVPTHFA F+T +  +++G+E KTLLNRLGTT DMAAA AFLASDD+SYITGET
Sbjct: 184 NAVAPGFVPTHFASFITGSSEVREGIEEKTLLNRLGTTGDMAAAAAFLASDDSSYITGET 243

Query: 245 LVVAGGMSSRL 256
           LVVAGGM SRL
Sbjct: 244 LVVAGGMPSRL 254

BLAST of MS013310 vs. ExPASy Swiss-Prot
Match: Q9BTZ2 (Dehydrogenase/reductase SDR family member 4 OS=Homo sapiens OX=9606 GN=DHRS4 PE=1 SV=3)

HSP 1 Score: 233.0 bits (593), Expect = 3.7e-60
Identity = 130/246 (52.85%), Postives = 168/246 (68.29%), Query Frame = 0

Query: 13  KVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHVSN 72
           KVA+VTAST GIGF IA RLA +GA VV+SSR+Q+NVD+AV  L+  GL V G VCHV  
Sbjct: 33  KVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGK 92

Query: 73  AQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLLQEV 132
           A+ R+ LV   ++ +G ID++VSNAAVNP   SI+   E V DK  DINVKA  L+ + V
Sbjct: 93  AEDRERLVATAVKLHGGIDILVSNAAVNPFFGSIMDVTEEVWDKTLDINVKAPALMTKAV 152

Query: 133 APHLQK--GSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP-DTRVNCVAP 192
            P ++K  G SVV++SSI  + PS   + Y V+KTALLGLTK LA E+AP + RVNC+AP
Sbjct: 153 VPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCLAP 212

Query: 193 GFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLVVAG 252
           G + T F+  L  +   ++ ++    + RLG  ED A   +FL S+DASYITGET+VV G
Sbjct: 213 GLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDASYITGETVVVGG 272

Query: 253 GMSSRL 256
           G  SRL
Sbjct: 273 GTPSRL 278

BLAST of MS013310 vs. ExPASy Swiss-Prot
Match: Q8WNV7 (Dehydrogenase/reductase SDR family member 4 OS=Sus scrofa OX=9823 GN=DHRS4 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 6.3e-60
Identity = 127/251 (50.60%), Postives = 170/251 (67.73%), Query Frame = 0

Query: 8   KRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVV 67
           K  + KVA+VTAST GIG  IA RLA +GA VV+SSR+Q+NVD  V  L+  GL V G V
Sbjct: 29  KPLENKVALVTASTDGIGLAIARRLAQDGAHVVVSSRKQENVDRTVATLQGEGLSVTGTV 88

Query: 68  CHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVL 127
           CHV  A+ R+ LV   +  +G +D++VSNAAVNP   +I+   E V DK+  +NVKA+VL
Sbjct: 89  CHVGKAEDRERLVAMAVNLHGGVDILVSNAAVNPFFGNIIDATEEVWDKILHVNVKATVL 148

Query: 128 LLQEVAPHLQK--GSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP-DTRV 187
           + + V P ++K  G SV+++SS+  Y P   L  Y V+KTALLGLTK LA E+AP + RV
Sbjct: 149 MTKAVVPEMEKRGGGSVLIVSSVGAYHPFPNLGPYNVSKTALLGLTKNLAVELAPRNIRV 208

Query: 188 NCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGET 247
           NC+APG + T+F+  L  + A K+ ++    + RLG  ED A   +FL S+DASYITGET
Sbjct: 209 NCLAPGLIKTNFSQVLWMDKARKEYMKESLRIRRLGNPEDCAGIVSFLCSEDASYITGET 268

Query: 248 LVVAGGMSSRL 256
           +VV GG +SRL
Sbjct: 269 VVVGGGTASRL 279

BLAST of MS013310 vs. ExPASy Swiss-Prot
Match: Q5RCF8 (Dehydrogenase/reductase SDR family member 4 OS=Pongo abelii OX=9601 GN=DHRS4 PE=2 SV=3)

HSP 1 Score: 232.3 bits (591), Expect = 6.3e-60
Identity = 129/246 (52.44%), Postives = 168/246 (68.29%), Query Frame = 0

Query: 13  KVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHVSN 72
           KVA+VTAST GIGF IA RLA +GA VV+SSR+Q+NVD+AV  L+  GL V G VCHV  
Sbjct: 33  KVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGK 92

Query: 73  AQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLLQEV 132
           A+ R+ LV   ++ +G ID++VSNAAVNP   S++   E V DK  DINVKA  L+ + V
Sbjct: 93  AEDRERLVATAVKLHGGIDILVSNAAVNPFFGSLMDVTEEVWDKTLDINVKAPALMTKAV 152

Query: 133 APHLQK--GSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP-DTRVNCVAP 192
            P ++K  G SVV++SSI  + PS   + Y V+KTALLGLTK LA E+AP + RVNC+AP
Sbjct: 153 VPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCLAP 212

Query: 193 GFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLVVAG 252
           G + T F+  L  +   ++ ++    + RLG  ED A   +FL S+DASYITGET+VV G
Sbjct: 213 GLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDASYITGETVVVGG 272

Query: 253 GMSSRL 256
           G  SRL
Sbjct: 273 GTPSRL 278

BLAST of MS013310 vs. ExPASy TrEMBL
Match: A0A6J1CNC4 (tropinone reductase-like 3 OS=Momordica charantia OX=3673 GN=LOC111012595 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.2e-130
Identity = 254/255 (99.61%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG
Sbjct: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV GVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI
Sbjct: 61  LEVSGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGMSSRL
Sbjct: 241 TGETLVVAGGMSSRL 255

BLAST of MS013310 vs. ExPASy TrEMBL
Match: A0A1S3CAC1 (tropinone reductase-like 3 OS=Cucumis melo OX=3656 GN=LOC103498229 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 7.1e-123
Identity = 237/255 (92.94%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD  KIGKRF+GKVAIVTASTQGIGFQIA RLA EGASVVISSRRQKNVDEAVEKLKA G
Sbjct: 1   MDNGKIGKRFRGKVAIVTASTQGIGFQIARRLAFEGASVVISSRRQKNVDEAVEKLKAEG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI
Sbjct: 61  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQ+GSSVVLISSIEGYQPS+LLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQDVAPHLQRGSSVVLISSIEGYQPSSLLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFL +N+A++KGVE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 DTRVNCVAPGFVPTHFADFLVTNEAVRKGVESKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 241 TGETLVVAGGMPSRL 255

BLAST of MS013310 vs. ExPASy TrEMBL
Match: A0A6J1EBY0 (tropinone reductase-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111432646 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.8e-122
Identity = 235/255 (92.16%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD TK+ KRFQGKVAIVTASTQGIGF+IA RLA+EGASVVISSRRQ+NVDEAVEKLKA G
Sbjct: 20  MDNTKMAKRFQGKVAIVTASTQGIGFRIAHRLALEGASVVISSRRQRNVDEAVEKLKAEG 79

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSIL+TQE++LDKLWDI
Sbjct: 80  LEVLGIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILRTQEAILDKLWDI 139

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQKGSSVVLISSIEG+QPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 140 NVKASVLLLQDVAPHLQKGSSVVLISSIEGFQPSALLAMYGVTKTALLGLTKALAAEMAP 199

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           DTRVNCVAPGFVPTHFADFL SN+AIKK VE+KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 200 DTRVNCVAPGFVPTHFADFLVSNEAIKKEVESKTLLNRLGTTEDMAAATAFLASDDASYI 259

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 260 TGETLVVAGGMPSRL 274

BLAST of MS013310 vs. ExPASy TrEMBL
Match: A0A6J1K1F0 (tropinone reductase-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111490216 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 234/255 (91.76%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MDYTKIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARG 60
           MD TK+ KRFQGKVAIVTASTQGIGF+IA+RLA+EGASVVISSRRQ+NVDEAVEKLKA G
Sbjct: 1   MDNTKMAKRFQGKVAIVTASTQGIGFRIAQRLALEGASVVISSRRQRNVDEAVEKLKAEG 60

Query: 61  LEVFGVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDI 120
           LEV G++CHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSIL+TQE+VLDKLWDI
Sbjct: 61  LEVLGIICHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILRTQEAVLDKLWDI 120

Query: 121 NVKASVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAP 180
           NVKASVLLLQ+VAPHLQKGSSVVLISSIEG+QPSALLAMYGVTKTALLGLTKALAAEMAP
Sbjct: 121 NVKASVLLLQDVAPHLQKGSSVVLISSIEGFQPSALLAMYGVTKTALLGLTKALAAEMAP 180

Query: 181 DTRVNCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYI 240
           +TRVNCVAPGFVPTHFADFL SN+AIKK VE KTLLNRLGTTEDMAAATAFLASDDASYI
Sbjct: 181 NTRVNCVAPGFVPTHFADFLVSNEAIKKEVELKTLLNRLGTTEDMAAATAFLASDDASYI 240

Query: 241 TGETLVVAGGMSSRL 256
           TGETLVVAGGM SRL
Sbjct: 241 TGETLVVAGGMPSRL 255

BLAST of MS013310 vs. ExPASy TrEMBL
Match: A0A0A0LYF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G698530 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.3e-120
Identity = 232/251 (92.43%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 5   KIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVF 64
           KIGKRF+GKVAIVTASTQGIGF+IA RLA EGASVVISSRRQ+NVDEAVEKLKA+GLEV 
Sbjct: 5   KIGKRFEGKVAIVTASTQGIGFEIARRLAFEGASVVISSRRQRNVDEAVEKLKAQGLEVL 64

Query: 65  GVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKA 124
           G+VCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKT+ESVLDKLWDINVKA
Sbjct: 65  GIVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTKESVLDKLWDINVKA 124

Query: 125 SVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDTRV 184
           SVLLLQ+VAPHLQKGSSVVLISSIEGY PS+LLAMYGVTKTALLGLTKALAAEMAPDTRV
Sbjct: 125 SVLLLQDVAPHLQKGSSVVLISSIEGYNPSSLLAMYGVTKTALLGLTKALAAEMAPDTRV 184

Query: 185 NCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGET 244
           NCVAPGFVPTHFADFL +N+AI+KGVE+KTLLNRLGTTEDMAAATAFLASDDASYITGE 
Sbjct: 185 NCVAPGFVPTHFADFLVTNEAIRKGVESKTLLNRLGTTEDMAAATAFLASDDASYITGEI 244

Query: 245 LVVAGGMSSRL 256
           LVVAGGM SRL
Sbjct: 245 LVVAGGMPSRL 255

BLAST of MS013310 vs. TAIR 10
Match: AT4G05530.1 (indole-3-butyric acid response 1 )

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-103
Identity = 186/251 (74.10%), Postives = 221/251 (88.05%), Query Frame = 0

Query: 5   KIGKRFQGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVF 64
           K+ +R +GKVAIVTASTQGIGF I ER  +EGASVV+SSR+Q NVDEAV KLK++G++ +
Sbjct: 4   KLPRRLEGKVAIVTASTQGIGFGITERFGLEGASVVVSSRKQANVDEAVAKLKSKGIDAY 63

Query: 65  GVVCHVSNAQQRKNLVEKTIQKYGKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKA 124
           G+VCHVSNAQ R+NLVEKT+QKYGKID+VV NAA NPS D IL ++E+VLDKLW+INVK+
Sbjct: 64  GIVCHVSNAQHRRNLVEKTVQKYGKIDIVVCNAAANPSTDPILSSKEAVLDKLWEINVKS 123

Query: 125 SVLLLQEVAPHLQKGSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDTRV 184
           S+LLLQ++APHL+KGSSV+ I+SI G+ P   +AMYGVTKTALLGLTKALAAEMAPDTRV
Sbjct: 124 SILLLQDMAPHLEKGSSVIFITSIAGFSPQGAMAMYGVTKTALLGLTKALAAEMAPDTRV 183

Query: 185 NCVAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGET 244
           N VAPGFVPTHFA F+T +  +++G+E KTLLNRLGTT DMAAA AFLASDD+SYITGET
Sbjct: 184 NAVAPGFVPTHFASFITGSSEVREGIEEKTLLNRLGTTGDMAAAAAFLASDDSSYITGET 243

Query: 245 LVVAGGMSSRL 256
           LVVAGGM SRL
Sbjct: 244 LVVAGGMPSRL 254

BLAST of MS013310 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-29
Identity = 85/245 (34.69%), Postives = 133/245 (54.29%), Query Frame = 0

Query: 12  GKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHVS 71
           GK A+VT  T+GIG  + E LA  GA V   SR Q+ ++  +   KA GL V G VC  S
Sbjct: 11  GKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGSVCDAS 70

Query: 72  NAQQRKNLVEKTIQKY-GKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLLQ 131
              QR+ L+++    + GK++++++N   N    ++  + E    K+   N++++  L Q
Sbjct: 71  VRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYA-KIMSTNLESAFHLSQ 130

Query: 132 EVAPHLQKG--SSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDT-RVNCV 191
              P L+     S+V ISS+ G    +  ++YG TK AL  LT+ LA E A D  R NCV
Sbjct: 131 IAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCV 190

Query: 192 APGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLVV 251
           AP ++ T   + L       + V ++T L R+G  E++++  AFL    +SYITG+ + V
Sbjct: 191 APWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISV 250

Query: 252 AGGMS 253
            GG +
Sbjct: 251 DGGFT 254

BLAST of MS013310 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-27
Identity = 84/246 (34.15%), Postives = 137/246 (55.69%), Query Frame = 0

Query: 11  QGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHV 70
           +G  A+VT  ++G+G  + E LA+ GA V   +R +  + E + + +A+G EV   VC V
Sbjct: 17  EGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTSVCDV 76

Query: 71  SNAQQRKNLVEKTIQKY-GKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLL 130
           S+ +QR+ L+E     + GK++++V+NA       S   T E     L   N++++  L 
Sbjct: 77  SSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAED-YSFLMATNLESAFHLS 136

Query: 131 QEVAPHLQK--GSSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPDT-RVNC 190
           Q   P L+     S+V +SS+ G   +   ++YG +K A+  L ++LA E A D  RVN 
Sbjct: 137 QIAHPLLKASGSGSIVFMSSVAGLVHTG-ASIYGASKGAMNQLGRSLACEWASDNIRVNS 196

Query: 191 VAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLV 250
           V P  + T    F+ S++ ++K VE KT + R+G   ++++  AFL    ASYITG+T+ 
Sbjct: 197 VCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITGQTIC 256

Query: 251 VAGGMS 253
           V GG S
Sbjct: 257 VDGGAS 260

BLAST of MS013310 vs. TAIR 10
Match: AT2G29340.2 (NAD-dependent epimerase/dehydratase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 8.0e-26
Identity = 79/246 (32.11%), Postives = 135/246 (54.88%), Query Frame = 0

Query: 11  QGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHV 70
           +G  A+VT    GIG+ I E LA  GA + +    +  +++++ + + +G +V G VC V
Sbjct: 8   KGMTALVTGGASGIGYAIVEELAGFGARIHVCDISEAKLNQSLSEWEKKGFQVSGSVCDV 67

Query: 71  SNAQQRKNLVEKTIQKY-GKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLL 130
           ++  +R+ L++    ++ GK++++VSN  V  S  +   T++     +   NV+A+    
Sbjct: 68  ASRPEREELMQTVSSQFDGKLNILVSNVGVIRSKPTTEYTEDDFAFHI-SSNVEAAYHFS 127

Query: 131 QEVAPHLQKG--SSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPD-TRVNC 190
           Q   P L+     S++ +SSI G       ++YG+TK AL+ L K LA E A D  R N 
Sbjct: 128 QLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGIRANA 187

Query: 191 VAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLV 250
           VAP  + T  +     + + KK + ++T L R+G   ++A+  AFL    ASYITG+T+ 
Sbjct: 188 VAPNVINTPLSQSYLEDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITGQTIC 247

Query: 251 VAGGMS 253
           V GG++
Sbjct: 248 VDGGLT 252

BLAST of MS013310 vs. TAIR 10
Match: AT2G29340.1 (NAD-dependent epimerase/dehydratase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 8.0e-26
Identity = 79/246 (32.11%), Postives = 135/246 (54.88%), Query Frame = 0

Query: 11  QGKVAIVTASTQGIGFQIAERLAVEGASVVISSRRQKNVDEAVEKLKARGLEVFGVVCHV 70
           +G  A+VT    GIG+ I E LA  GA + +    +  +++++ + + +G +V G VC V
Sbjct: 8   KGMTALVTGGASGIGYAIVEELAGFGARIHVCDISEAKLNQSLSEWEKKGFQVSGSVCDV 67

Query: 71  SNAQQRKNLVEKTIQKY-GKIDVVVSNAAVNPSVDSILKTQESVLDKLWDINVKASVLLL 130
           ++  +R+ L++    ++ GK++++VSN  V  S  +   T++     +   NV+A+    
Sbjct: 68  ASRPEREELMQTVSSQFDGKLNILVSNVGVIRSKPTTEYTEDDFAFHI-SSNVEAAYHFS 127

Query: 131 QEVAPHLQKG--SSVVLISSIEGYQPSALLAMYGVTKTALLGLTKALAAEMAPD-TRVNC 190
           Q   P L+     S++ +SSI G       ++YG+TK AL+ L K LA E A D  R N 
Sbjct: 128 QLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGIRANA 187

Query: 191 VAPGFVPTHFADFLTSNDAIKKGVEAKTLLNRLGTTEDMAAATAFLASDDASYITGETLV 250
           VAP  + T  +     + + KK + ++T L R+G   ++A+  AFL    ASYITG+T+ 
Sbjct: 188 VAPNVINTPLSQSYLEDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITGQTIC 247

Query: 251 VAGGMS 253
           V GG++
Sbjct: 248 VDGGLT 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142483.12.5e-13099.61tropinone reductase-like 3 [Momordica charantia][more]
XP_008458979.11.5e-12292.94PREDICTED: tropinone reductase-like 3 [Cucumis melo][more]
KAG6573495.11.2e-12192.55hypothetical protein SDJN03_27382, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022925358.11.6e-12192.16tropinone reductase-like 3 [Cucurbita moschata] >XP_022925359.1 tropinone reduct... [more]
XP_023525934.13.6e-12192.16tropinone reductase-like 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
H9BFQ21.5e-10679.60Tropinone reductase-like 3 OS=Erythroxylum coca OX=289672 PE=2 SV=1[more]
Q9S9W21.7e-10274.10Short-chain dehydrogenase/reductase SDRA OS=Arabidopsis thaliana OX=3702 GN=SDRA... [more]
Q9BTZ23.7e-6052.85Dehydrogenase/reductase SDR family member 4 OS=Homo sapiens OX=9606 GN=DHRS4 PE=... [more]
Q8WNV76.3e-6050.60Dehydrogenase/reductase SDR family member 4 OS=Sus scrofa OX=9823 GN=DHRS4 PE=1 ... [more]
Q5RCF86.3e-6052.44Dehydrogenase/reductase SDR family member 4 OS=Pongo abelii OX=9601 GN=DHRS4 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CNC41.2e-13099.61tropinone reductase-like 3 OS=Momordica charantia OX=3673 GN=LOC111012595 PE=4 S... [more]
A0A1S3CAC17.1e-12392.94tropinone reductase-like 3 OS=Cucumis melo OX=3656 GN=LOC103498229 PE=4 SV=1[more]
A0A6J1EBY07.8e-12292.16tropinone reductase-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111432646 PE=4 SV... [more]
A0A6J1K1F03.0e-12191.76tropinone reductase-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111490216 PE=4 SV=1[more]
A0A0A0LYF93.3e-12092.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G698530 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G05530.11.2e-10374.10indole-3-butyric acid response 1 [more]
AT5G06060.12.0e-2934.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29150.11.9e-2734.15NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29340.28.0e-2632.11NAD-dependent epimerase/dehydratase family protein [more]
AT2G29340.18.0e-2632.11NAD-dependent epimerase/dehydratase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 140..148
score: 30.19
coord: 88..99
score: 37.93
coord: 160..179
score: 35.79
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 14..31
score: 42.81
coord: 134..150
score: 24.46
coord: 215..235
score: 36.79
coord: 88..99
score: 46.62
coord: 180..197
score: 39.03
coord: 160..179
score: 40.41
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 22..252
e-value: 2.7E-61
score: 207.1
NoneNo IPR availableGENE3D3.40.50.720coord: 3..254
e-value: 8.2E-76
score: 256.6
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..205
e-value: 3.6E-15
score: 53.8
NoneNo IPR availablePANTHERPTHR43943DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4coord: 5..255
NoneNo IPR availablePANTHERPTHR43943:SF14TROPINONE REDUCTASE-LIKE 3coord: 5..255
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 147..175
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 11..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013310.1MS013310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity