MS013213 (gene) Bitter gourd (TR) v1

BLAST of MS013213 vs. NCBI nr
Match: XP_022148094.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like [Momordica charantia])

HSP 1 Score: 505.0 bits (1299), Expect = 3.7e-139
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKYNKV
Sbjct: 241 QKYKYNKV 248

BLAST of MS013213 vs. NCBI nr
Match: XP_038899626.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic [Benincasa hispida])

HSP 1 Score: 476.9 bits (1226), Expect = 1.1e-130
Identity = 234/248 (94.35%), Postives = 237/248 (95.56%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SVQSVCL SNSS+AHF PR  K LV TRLS+RASL DNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAASVQSVCLFSNSSTAHFHPRSTKPLVSTRLSIRASLPDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAAHYQIPLRHILL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 241 QKYKYHKV 248

BLAST of MS013213 vs. NCBI nr
Match: XP_022998092.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 470.3 bits (1209), Expect = 1.0e-128
Identity = 232/248 (93.55%), Postives = 235/248 (94.76%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SV SVCL SNSSSAHF  R  KRLVPTRLSVRASL DNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAASVPSVCLFSNSSSAHFPSRFPKRLVPTRLSVRASLQDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLA HYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLAL GFS+ V+RFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 241 QKYKYHKV 248

BLAST of MS013213 vs. NCBI nr
Match: XP_023523313.1 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 467.6 bits (1202), Expect = 6.6e-128
Identity = 231/248 (93.15%), Postives = 234/248 (94.35%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SV SVCL SNSS AHF  R  KRLVPTRLSVRASL DNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAASVPSVCLFSNSSIAHFPSRFPKRLVPTRLSVRASLPDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLA HYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLAL GFS+ V+RFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 241 QKYKYHKV 248

BLAST of MS013213 vs. NCBI nr
Match: KAG6607055.1 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 466.1 bits (1198), Expect = 1.9e-127
Identity = 230/248 (92.74%), Postives = 234/248 (94.35%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SV SVCL SNSS AHF  R  KRLVPTRLSVRASL DNDNGVKVEYTPWLIVGLG
Sbjct: 47  MLYAASVPSVCLFSNSSIAHFPSRFPKRLVPTRLSVRASLPDNDNGVKVEYTPWLIVGLG 106

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 107 NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 166

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLA HYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 167 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 226

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMD+KAFLLQKF SVERKQIDAALEQGVEAVRTLAL GFS+ V+RFNLT
Sbjct: 227 RLCIGIGNPPGTMDIKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 286

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 287 QKYKYHKV 294

BLAST of MS013213 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.0e-94
Identity = 162/205 (79.02%), Postives = 185/205 (90.24%), Query Frame = 0

Query: 44  DNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSI 103
           D+  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID +A+ + +LMNTIQSKALIGIG+I
Sbjct: 36  DDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAI 95

Query: 104 GEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHH 163
            +VPILLAKPQTYMNF GESVG LA+HY++PLRHIL++YDEM+LPNGVLRLQPKGG G+H
Sbjct: 96  EDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYH 155

Query: 164 NGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVR 223
           NGVKSVMGHLDGRR FPRL IGIG PPG MDMKAFLLQKF  +E+KQI+ ALEQG EAV+
Sbjct: 156 NGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVK 215

Query: 224 TLALNGFSQRVNRFNLTQKYKYNKV 249
           TL LNGF+Q ++RFNL QKYK++KV
Sbjct: 216 TLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of MS013213 vs. ExPASy Swiss-Prot
Match: Q9FKN4 (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2A PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.2e-89
Identity = 159/215 (73.95%), Postives = 182/215 (84.65%), Query Frame = 0

Query: 35  SVRASLLDNDNG-VKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQ 94
           S+RASL  +DN  +K EYTPWLIVGLGNPG KY+GTRHN+GFEMID IA+A DI MNTIQ
Sbjct: 32  SLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQ 91

Query: 95  SKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLR 154
           SKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRHIL++YD+M L NGVLR
Sbjct: 92  SKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLR 151

Query: 155 LQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDA 214
           LQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFLLQKF  +ERKQ+D 
Sbjct: 152 LQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDE 211

Query: 215 ALEQGVEAVRTLALNGFSQRVNRFNLTQKYKYNKV 249
            LEQGVE V+TL   GFS  ++RFNL QKYK++KV
Sbjct: 212 GLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 246

BLAST of MS013213 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 9.0e-88
Identity = 154/218 (70.64%), Postives = 182/218 (83.49%), Query Frame = 0

Query: 31  PTRLSVRASLLDNDNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMN 90
           P   +V +S+ D   G  VEYTPWLI GLGNPGNKY+GTRHNVGFEM+D IA  + I MN
Sbjct: 40  PRPFTVVSSVPDPAAG-PVEYTPWLIAGLGNPGNKYYGTRHNVGFEMVDRIAAEEGITMN 99

Query: 91  TIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNG 150
           TIQSK+L+GIGSIGEVP+L+ KPQ+YMN+ GE++GPLAA+YQ+PLRHILL+YD+ SLPNG
Sbjct: 100 TIQSKSLLGIGSIGEVPVLVVKPQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNG 159

Query: 151 VLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQ 210
           VLRLQ KGGHG HNG+++V+ HLDGRREFPRL IGIG+PPG MD +AFLLQKF S ER Q
Sbjct: 160 VLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQ 219

Query: 211 IDAALEQGVEAVRTLALNGFSQRVNRFNLTQKYKYNKV 249
           ID ALEQGV+AVRTL L GFS    RFNL QKYK+++V
Sbjct: 220 IDTALEQGVDAVRTLVLKGFSGSTERFNLVQKYKFHRV 256

BLAST of MS013213 vs. ExPASy Swiss-Prot
Match: Q5ZCL8 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.5e-86
Identity = 157/237 (66.24%), Postives = 190/237 (80.17%), Query Frame = 0

Query: 12  LLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLGNPGNKYHGTRH 71
           LL ++++   R RLR+     R++V AS+ D       EYTPWLI GLGNPG+KYHGTRH
Sbjct: 29  LLLSAAAPLRRTRLRR-----RIAVVASVPD-PAARPAEYTPWLIAGLGNPGSKYHGTRH 88

Query: 72  NVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHY 131
           NVGFEM+D IA+ + I MNTIQSK+L+GIGSIGEVP+LL KPQ+Y+N+ GE++GPLAA+Y
Sbjct: 89  NVGFEMVDRIARDEGITMNTIQSKSLLGIGSIGEVPVLLVKPQSYINYSGEAIGPLAAYY 148

Query: 132 QIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPG 191
           Q+PLRHIL++YDEMSLPNGVLRLQ KGGHG HNG+++VM  LD  RE PRL IGIG+PPG
Sbjct: 149 QVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPG 208

Query: 192 TMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLTQKYKYNKV 249
            MD +AFLLQKF S ER QID ALEQGV+AVRTL L GFS  + RFNL QKYK++ V
Sbjct: 209 KMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQKYKFHSV 259

BLAST of MS013213 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-64
Identity = 114/197 (57.87%), Postives = 147/197 (74.62%), Query Frame = 0

Query: 51  YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILL 110
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++  I MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 111 AKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 170
           AKPQTYMN  GES GPLAA+Y++PL  +L+V+D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 171 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGF 230
            H  G REF RL IGIG PPG MD KAFLLQKF    R+++D AL +GV+A++ +    F
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDF 195

Query: 231 SQRVNRFNLTQKYKYNK 248
            +    FN+ QKYK+ K
Sbjct: 196 GESWRLFNVEQKYKHLK 212

BLAST of MS013213 vs. ExPASy TrEMBL
Match: A0A6J1D2Z6 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111016856 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.8e-139
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKYNKV
Sbjct: 241 QKYKYNKV 248

BLAST of MS013213 vs. ExPASy TrEMBL
Match: A0A6J1K6X4 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492843 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 4.9e-129
Identity = 232/248 (93.55%), Postives = 235/248 (94.76%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SV SVCL SNSSSAHF  R  KRLVPTRLSVRASL DNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAASVPSVCLFSNSSSAHFPSRFPKRLVPTRLSVRASLQDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLA HYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLAL GFS+ V+RFNLT
Sbjct: 181 RLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 241 QKYKYHKV 248

BLAST of MS013213 vs. ExPASy TrEMBL
Match: A0A6J1G9M7 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452203 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.1e-127
Identity = 229/248 (92.34%), Postives = 234/248 (94.35%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDNDNGVKVEYTPWLIVGLG 60
           MLYA SV SVCL SNSS AHF  R  KRLVPTRLSVRASL DNDNGVKVEYTPWLIVGLG
Sbjct: 1   MLYAASVPSVCLFSNSSIAHFPSRFPKRLVPTRLSVRASLPDNDNGVKVEYTPWLIVGLG 60

Query: 61  NPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120
           NPGNKYHGTRHNVGF+MIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC
Sbjct: 61  NPGNKYHGTRHNVGFDMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNFC 120

Query: 121 GESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180
           GESVGPLA HYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP
Sbjct: 121 GESVGPLAVHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREFP 180

Query: 181 RLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNLT 240
           RLCIGIGNPPGTMD+KAFLLQKF SVERKQIDAALEQGVEAVRTLAL GFS+ V+RFNLT
Sbjct: 181 RLCIGIGNPPGTMDIKAFLLQKFSSVERKQIDAALEQGVEAVRTLALKGFSKGVDRFNLT 240

Query: 241 QKYKYNKV 249
           QKYKY+KV
Sbjct: 241 QKYKYHKV 248

BLAST of MS013213 vs. ExPASy TrEMBL
Match: A0A1S3CFG5 (chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500129 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 7.4e-125
Identity = 228/250 (91.20%), Postives = 233/250 (93.20%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASL--LDNDNGVKVEYTPWLIVG 60
           MLYA S QSV L SNSS+AH+     K LV TRLSVRASL   DN+NGVKVEYTPWLIVG
Sbjct: 1   MLYAASFQSVSLFSNSSTAHYHSPSTKPLVSTRLSVRASLPDSDNNNGVKVEYTPWLIVG 60

Query: 61  LGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMN 120
           LGNPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMN
Sbjct: 61  LGNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMN 120

Query: 121 FCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180
           FCGESVGPLAAHYQIPLRHILL YDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE
Sbjct: 121 FCGESVGPLAAHYQIPLRHILLTYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRRE 180

Query: 181 FPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFN 240
           FPRLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLALNGFSQRVNRFN
Sbjct: 181 FPRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFN 240

Query: 241 LTQKYKYNKV 249
           L+QKYKY+KV
Sbjct: 241 LSQKYKYHKV 250

BLAST of MS013213 vs. ExPASy TrEMBL
Match: A0A0A0LCC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G257600 PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.5e-122
Identity = 224/249 (89.96%), Postives = 231/249 (92.77%), Query Frame = 0

Query: 1   MLYAVSVQSVCLLSNSSSAHFRPRLRKRLVPTRLSVRASLLDND-NGVKVEYTPWLIVGL 60
           ML+A S QSV L SNSSSAHF     K LV TRLSVRAS  D+D NG+KVEYTPWLIVGL
Sbjct: 1   MLFAASFQSVSLFSNSSSAHFHFPSTKPLVSTRLSVRASSPDSDSNGLKVEYTPWLIVGL 60

Query: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120
           GNPGNKYHGTRHNVGFEMIDSIAQAQ ILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF
Sbjct: 61  GNPGNKYHGTRHNVGFEMIDSIAQAQGILMNTIQSKALIGIGSIGEVPILLAKPQTYMNF 120

Query: 121 CGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVMGHLDGRREF 180
           CGESVGPLAAHYQIPLRH+LL YDEM+LPNGVLRLQPK GHGHHNGVKSVMGHLDGRREF
Sbjct: 121 CGESVGPLAAHYQIPLRHVLLTYDEMNLPNGVLRLQPKAGHGHHNGVKSVMGHLDGRREF 180

Query: 181 PRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNL 240
           PRLCIGIGNPPGTMDMKAFLLQKF SVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNL
Sbjct: 181 PRLCIGIGNPPGTMDMKAFLLQKFSSVERKQIDAALEQGVEAVRTLALNGFSQRVNRFNL 240

Query: 241 TQKYKYNKV 249
           +QKYKY+KV
Sbjct: 241 SQKYKYHKV 249

BLAST of MS013213 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 347.8 bits (891), Expect = 7.1e-96
Identity = 162/205 (79.02%), Postives = 185/205 (90.24%), Query Frame = 0

Query: 44  DNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSI 103
           D+  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID +A+ + +LMNTIQSKALIGIG+I
Sbjct: 36  DDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAI 95

Query: 104 GEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHH 163
            +VPILLAKPQTYMNF GESVG LA+HY++PLRHIL++YDEM+LPNGVLRLQPKGG G+H
Sbjct: 96  EDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYH 155

Query: 164 NGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVR 223
           NGVKSVMGHLDGRR FPRL IGIG PPG MDMKAFLLQKF  +E+KQI+ ALEQG EAV+
Sbjct: 156 NGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVK 215

Query: 224 TLALNGFSQRVNRFNLTQKYKYNKV 249
           TL LNGF+Q ++RFNL QKYK++KV
Sbjct: 216 TLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of MS013213 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 347.8 bits (891), Expect = 7.1e-96
Identity = 162/205 (79.02%), Postives = 185/205 (90.24%), Query Frame = 0

Query: 44  DNGVKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSI 103
           D+  KVEYTPWLIVGLGNPGNKYHGTRHNVGFEMID +A+ + +LMNTIQSKALIGIG+I
Sbjct: 36  DDRFKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAI 95

Query: 104 GEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHH 163
            +VPILLAKPQTYMNF GESVG LA+HY++PLRHIL++YDEM+LPNGVLRLQPKGG G+H
Sbjct: 96  EDVPILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYH 155

Query: 164 NGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVR 223
           NGVKSVMGHLDGRR FPRL IGIG PPG MDMKAFLLQKF  +E+KQI+ ALEQG EAV+
Sbjct: 156 NGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVK 215

Query: 224 TLALNGFSQRVNRFNLTQKYKYNKV 249
           TL LNGF+Q ++RFNL QKYK++KV
Sbjct: 216 TLVLNGFNQGISRFNLVQKYKFHKV 240

BLAST of MS013213 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 330.1 bits (845), Expect = 1.5e-90
Identity = 159/215 (73.95%), Postives = 182/215 (84.65%), Query Frame = 0

Query: 35  SVRASLLDNDNG-VKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQ 94
           S+RASL  +DN  +K EYTPWLIVGLGNPG KY+GTRHN+GFEMID IA+A DI MNTIQ
Sbjct: 36  SLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQ 95

Query: 95  SKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLR 154
           SKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRHIL++YD+M L NGVLR
Sbjct: 96  SKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLR 155

Query: 155 LQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDA 214
           LQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFLLQKF  +ERKQ+D 
Sbjct: 156 LQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDE 215

Query: 215 ALEQGVEAVRTLALNGFSQRVNRFNLTQKYKYNKV 249
            LEQGVE V+TL   GFS  ++RFNL QKYK++KV
Sbjct: 216 GLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 250

BLAST of MS013213 vs. TAIR 10
Match: AT5G38290.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 330.1 bits (845), Expect = 1.5e-90
Identity = 159/215 (73.95%), Postives = 182/215 (84.65%), Query Frame = 0

Query: 35  SVRASLLDNDNG-VKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQ 94
           S+RASL  +DN  +K EYTPWLIVGLGNPG KY+GTRHN+GFEMID IA+A DI MNTIQ
Sbjct: 32  SLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQ 91

Query: 95  SKALIGIGSIGEVPILLAKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLR 154
           SKAL+GIGS+GEVPILL KPQ YMNF GESVGPLAA+YQIPLRHIL++YD+M L NGVLR
Sbjct: 92  SKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLR 151

Query: 155 LQPKGGHGHHNGVKSVMGHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDA 214
           LQPKGGH  HNG+K+V  HL+G R +PRL IGIGNPPG MDMKAFLLQKF  +ERKQ+D 
Sbjct: 152 LQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDE 211

Query: 215 ALEQGVEAVRTLALNGFSQRVNRFNLTQKYKYNKV 249
            LEQGVE V+TL   GFS  ++RFNL QKYK++KV
Sbjct: 212 GLEQGVEGVKTLVEEGFSDSISRFNLGQKYKFHKV 246

BLAST of MS013213 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 246.9 bits (629), Expect = 1.7e-65
Identity = 114/197 (57.87%), Postives = 147/197 (74.62%), Query Frame = 0

Query: 51  YTPWLIVGLGNPGNKYHGTRHNVGFEMIDSIAQAQDILMNTIQSKALIGIGSIGEVPILL 110
           + PWL +GLGNPG+KY GTRHN+GFEMID  A++  I MN +  KA++G G + ++P++L
Sbjct: 16  HRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVIL 75

Query: 111 AKPQTYMNFCGESVGPLAAHYQIPLRHILLVYDEMSLPNGVLRLQPKGGHGHHNGVKSVM 170
           AKPQTYMN  GES GPLAA+Y++PL  +L+V+D+M LP GVLRLQ KGGHG HNG+KSVM
Sbjct: 76  AKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVM 135

Query: 171 GHLDGRREFPRLCIGIGNPPGTMDMKAFLLQKFGSVERKQIDAALEQGVEAVRTLALNGF 230
            H  G REF RL IGIG PPG MD KAFLLQKF    R+++D AL +GV+A++ +    F
Sbjct: 136 NHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDF 195

Query: 231 SQRVNRFNLTQKYKYNK 248
            +    FN+ QKYK+ K
Sbjct: 196 GESWRLFNVEQKYKHLK 212