MS013160 (gene) Bitter gourd (TR) v1

Overview
NameMS013160
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAlpha-MPP
Locationscaffold459: 787453 .. 790976 (-)
RNA-Seq ExpressionMS013160
SyntenyMS013160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCATGCGGCCAACTTGGGGGCCTCTAGATTTGCAACATCCAGCGCAGTTGCTGCAAGACCAACATCGGGGGGCTTTTTTAGCTGGCTCACTGGAGAACGTTCAAGCTCTCTTCCCCCACTGGAAATGCCTCTATCTGGCATTACCCTCCCACCGCCATTGCCTGATTATGTCGAACCAAGCAAGACCAAGATTACCGCTCTTCCTAATGGTGTCAGAATTGCTTCGGAAACCTCTTCGGTATGTATGGAGCTTGAATGACATCTAGACTCTTTTTCTTAATGGCATCTAGACTCTTCTTGTTTTAAATTTAATAATGAACTTATTTATTTACCAAACTGACAAGTTTTAGACTGGTTGGTTAACCAGAATCCTGCAGCCTCTGTGGGGATATATCTTGATTGTGGTTCCATATATGAGACCCCATTGTCTTCCGGAGCCTCACATTTGTTAGAAAGGTTGGCCTTCAAGAGTACCACAAATCGTAGCCATTTGCGGATTGTTAGGGAAGTAGAAGCTATTGGTGGTAACACAGGTGCCTCAGCCTCTCGGGAGCAAATGGGATACACTCTTGATGCTTTAAAAACATATGTACCGGAAATGGTGGAATTGCTAGTAGACTGTGTCAGGAACCCTGCATACTTGGATTGGGAAATTAATGAAGAGGTAATCATTCATCTAGTTACCATTATAGAAGTTTGTCCGTCTGATAACTGAATATGAAAAATGATATTTTGGGCTTTTGTGTCCATTGCCAATATTTTGATTCCATATGTCCATACAGCTGCAAAAGATGAGAGCAGAACTTGGAGAACTTTCAAATAACCCCCAGGGCTTGCTTCTTGAGGCAATTCATTCTGCTGGGTATTCTGGTGCACTTGCAAATCCTCTCGTGGCCCCAGAGTTTGCGTTAAGCAGATTGGATGGCACCATTTTGGAGGAATTTATTGCTGTGAGACCTTTCATTCTTTTATGACTACTCTTCCTGTTTTTCCTTTCTTTTTCTCCATAGAGGAAGGCATACAACCTACTTTTCCCCCATATATCTTCTTAGCCTCCCCACATATGATAATTTCATTTCTTTGTTCCCTCTGTTTTCCTGTATTTTAATTTAAACTATATGATATTCCACCACTTTTGGATTTCAGGAGAACTATACTGCCCCTAGAATTGTACTTGCAGCATCTGGGGTTGAACATGAGGAGCTTTTATCCATTGCAGAACCTCTTCTATCTGACCTACCCAAAGTACCTAGCTCGAAAGCGCCAGAATCTGCCTATGTTGGAGGGGATTATCGTCGTCAATCTGACTCTGGGGTGTGTATAAACTATAACTGTATTGTAGATTATTTATTCACAATAGCCCTCAAAACCATGATTCAAAACATTGATGGAGGTATAGTGGATGTAACTTGGTTGAGTTATATACTTAAACCCATCTAATTGGCCAGCCATTTGAAAATAACATACGGTAGAAAAGAAAGGGGAAATACCAAAGGATTGAATTTTACTTAACCAAGACAAGTAATTTGGCCTTTGATTCCCGTGAATGGCTGAGTTAATGATAATGTGATCAATATACTATAACTTCACTGATGTACTGTTTCCCATTGCTCGATTTTTATATATTCACGTAGGCTTTTCATTTTAGCAGAGCACACATTTTGCCCTTGCTTTTGAGGTTCCTGGTGGTTGGCTTAAGGAGAAAGATGCCATCGTTTTGACTGTTCTTCAGGTGCCTCGTTTTATTACTTTTTGGTCTCGTAAAGATGTTCAATTTGTCTTGTTGGATTGTTTTAAAAAATGGAGGAGAATGGAAACGTGTTCGAGTACTCATATTAGGGGGGAAAAAACATGGTTAAATGATTTCTTGTTTGATGAGTTCTATTAACATCTCTCAGTCCTGCAGATGCTAATGGGAGGCGGTGGTTCATTTTCTGCTGGGGGTCCTGGTAAAGGAATGCACTCAAGGCTATGTAAGTATCACCTAGGTTTTCAGTTTAACGATTAGATTTTATCAACCTTTTCCTTTCCCTTTTTATCCATGGTAATTCGTTCTCTTTGCTAGTTTTTATCTATATGAGTTCCTTTATTCACTCCTATATCTGTTGTTATTTGCTGCTGTTAAGTCTTTTCTTTTGATTTTGGATTCAAAACAGATCTCCGTGTCTTGAATGAGTACCAACAGATTCAGTCTTTTTCTGCATTTAACAGCATCTTCAATAACACGGGACTATTTGGCATTTATGGAAGCACTGTAAGGCACAATCTTACAATTGATTTTTTATATTATTAATGTACTAATGCTGCCAGTTCGATGGTTAATTAACCAGAAGAAGGGGGAGAGGGAAACAAGGTTACGTGTTAGCTGCAAATTATATGGGGAGCTATGATAGGGACCAAATAATTGGAATATATTAAAAAGATAGAAAAATTGAAGAACTACAATAATTCTCAAGTATTCTTATTCAAGCCTAAAGCCACTGCACAATAAACGTACCTCCATCCTTTTCTCCCGACTCTATTTTTAACAAATTCTGCTAATTGCTAGTATACCCTTATTGTTCTATTAATATGCCTAATTAGCCAAAGGCATTCCCGTCAAGCTACTGCATGATTTTAGCTCGCCTTTGTTTTAATTTTTCATGTTATGTTCTTACCTATCATAACTACAATTGTGAATATCCAATTTGTGGATGCAGAGTTCTGACTTCGTGGCCAAAGCAGTTGATATTGCTGCTTCAGAACTAATCTCAATTGCAACTCCTGGGCAAGGTTTGCAGATTTTTGTTTTGTGTTCGGGTTCTTTTTAATTACTCTGTTTATATATCTTGCTCCCTGTTTTTCGTGGCAGTTTCCGAGGTGCAGCTTAACCGCGCCAAAGAGTCTACAAAATCTGCAGTTTTAATGAATCTAGAATCTAGGGTACACTCTCTCTTCCTCTCACACGCACATATCATTTTTTCTTTGCCATGGGCTGTTATTCTCTTTGCTTTTCTCTTCCATGCCTGATGTAGCAGAACCTTTCATTAAAAGAGAGTAAAACAGAGACCAAAGTAAGCTGACACTTGATTATCCTGCAGATGATTGTGTCCGAGGATATTGGGAGGCAGATTTTGACATACGGAGAGAGGTATCTTTTATGATCGAACTAGCTCTACTACCGAGCACGTCTTTCTTATAATCCATTTTCATATTTCCAATGTTATTGGATTTCTCTGCAGTTTTAAGTTATGGGTCGTCATGTTTTTCAATATTGCAGGAAGCCTGTAGAGCACTTCATAAAGGCTGTAGATTCAATCACTTTGAACGATATTACTAACATTTCCCAGAAAGTTTTATCATCTCCTTTAACAATGGCATCCTACGGTGACGGTAATCTTCAACTTCTTCATCTTTTCCATCGAATTAACTAATTTCGATACAATCATATCTTTAAGACTGACATCTAGAAATTTCATCATTGGCATTGCAGTTATTAATGTCCCGACGTACGAGTCTGTTAACAGCAAGTTCCACCGAAAA

mRNA sequence

AACCATGCGGCCAACTTGGGGGCCTCTAGATTTGCAACATCCAGCGCAGTTGCTGCAAGACCAACATCGGGGGGCTTTTTTAGCTGGCTCACTGGAGAACGTTCAAGCTCTCTTCCCCCACTGGAAATGCCTCTATCTGGCATTACCCTCCCACCGCCATTGCCTGATTATGTCGAACCAAGCAAGACCAAGATTACCGCTCTTCCTAATGGTGTCAGAATTGCTTCGGAAACCTCTTCGAATCCTGCAGCCTCTGTGGGGATATATCTTGATTGTGGTTCCATATATGAGACCCCATTGTCTTCCGGAGCCTCACATTTGTTAGAAAGGTTGGCCTTCAAGAGTACCACAAATCGTAGCCATTTGCGGATTGTTAGGGAAGTAGAAGCTATTGGTGGTAACACAGGTGCCTCAGCCTCTCGGGAGCAAATGGGATACACTCTTGATGCTTTAAAAACATATGTACCGGAAATGGTGGAATTGCTAGTAGACTGTGTCAGGAACCCTGCATACTTGGATTGGGAAATTAATGAAGAGCTGCAAAAGATGAGAGCAGAACTTGGAGAACTTTCAAATAACCCCCAGGGCTTGCTTCTTGAGGCAATTCATTCTGCTGGGTATTCTGGTGCACTTGCAAATCCTCTCGTGGCCCCAGAGTTTGCGTTAAGCAGATTGGATGGCACCATTTTGGAGGAATTTATTGCTGAGAACTATACTGCCCCTAGAATTGTACTTGCAGCATCTGGGGTTGAACATGAGGAGCTTTTATCCATTGCAGAACCTCTTCTATCTGACCTACCCAAAGTACCTAGCTCGAAAGCGCCAGAATCTGCCTATGTTGGAGGGGATTATCGTCGTCAATCTGACTCTGGGAGCACACATTTTGCCCTTGCTTTTGAGGTTCCTGGTGGTTGGCTTAAGGAGAAAGATGCCATCGTTTTGACTGTTCTTCAGATGCTAATGGGAGGCGGTGGTTCATTTTCTGCTGGGGGTCCTGGTAAAGGAATGCACTCAAGGCTATATCTCCGTGTCTTGAATGAGTACCAACAGATTCAGTCTTTTTCTGCATTTAACAGCATCTTCAATAACACGGGACTATTTGGCATTTATGGAAGCACTAGTTCTGACTTCGTGGCCAAAGCAGTTGATATTGCTGCTTCAGAACTAATCTCAATTGCAACTCCTGGGCAAGTTTCCGAGGTGCAGCTTAACCGCGCCAAAGAGTCTACAAAATCTGCAGTTTTAATGAATCTAGAATCTAGGATGATTGTGTCCGAGGATATTGGGAGGCAGATTTTGACATACGGAGAGAGGAAGCCTGTAGAGCACTTCATAAAGGCTGTAGATTCAATCACTTTGAACGATATTACTAACATTTCCCAGAAAGTTTTATCATCTCCTTTAACAATGGCATCCTACGGTGACGTTATTAATGTCCCGACGTACGAGTCTGTTAACAGCAAGTTCCACCGAAAA

Coding sequence (CDS)

AACCATGCGGCCAACTTGGGGGCCTCTAGATTTGCAACATCCAGCGCAGTTGCTGCAAGACCAACATCGGGGGGCTTTTTTAGCTGGCTCACTGGAGAACGTTCAAGCTCTCTTCCCCCACTGGAAATGCCTCTATCTGGCATTACCCTCCCACCGCCATTGCCTGATTATGTCGAACCAAGCAAGACCAAGATTACCGCTCTTCCTAATGGTGTCAGAATTGCTTCGGAAACCTCTTCGAATCCTGCAGCCTCTGTGGGGATATATCTTGATTGTGGTTCCATATATGAGACCCCATTGTCTTCCGGAGCCTCACATTTGTTAGAAAGGTTGGCCTTCAAGAGTACCACAAATCGTAGCCATTTGCGGATTGTTAGGGAAGTAGAAGCTATTGGTGGTAACACAGGTGCCTCAGCCTCTCGGGAGCAAATGGGATACACTCTTGATGCTTTAAAAACATATGTACCGGAAATGGTGGAATTGCTAGTAGACTGTGTCAGGAACCCTGCATACTTGGATTGGGAAATTAATGAAGAGCTGCAAAAGATGAGAGCAGAACTTGGAGAACTTTCAAATAACCCCCAGGGCTTGCTTCTTGAGGCAATTCATTCTGCTGGGTATTCTGGTGCACTTGCAAATCCTCTCGTGGCCCCAGAGTTTGCGTTAAGCAGATTGGATGGCACCATTTTGGAGGAATTTATTGCTGAGAACTATACTGCCCCTAGAATTGTACTTGCAGCATCTGGGGTTGAACATGAGGAGCTTTTATCCATTGCAGAACCTCTTCTATCTGACCTACCCAAAGTACCTAGCTCGAAAGCGCCAGAATCTGCCTATGTTGGAGGGGATTATCGTCGTCAATCTGACTCTGGGAGCACACATTTTGCCCTTGCTTTTGAGGTTCCTGGTGGTTGGCTTAAGGAGAAAGATGCCATCGTTTTGACTGTTCTTCAGATGCTAATGGGAGGCGGTGGTTCATTTTCTGCTGGGGGTCCTGGTAAAGGAATGCACTCAAGGCTATATCTCCGTGTCTTGAATGAGTACCAACAGATTCAGTCTTTTTCTGCATTTAACAGCATCTTCAATAACACGGGACTATTTGGCATTTATGGAAGCACTAGTTCTGACTTCGTGGCCAAAGCAGTTGATATTGCTGCTTCAGAACTAATCTCAATTGCAACTCCTGGGCAAGTTTCCGAGGTGCAGCTTAACCGCGCCAAAGAGTCTACAAAATCTGCAGTTTTAATGAATCTAGAATCTAGGATGATTGTGTCCGAGGATATTGGGAGGCAGATTTTGACATACGGAGAGAGGAAGCCTGTAGAGCACTTCATAAAGGCTGTAGATTCAATCACTTTGAACGATATTACTAACATTTCCCAGAAAGTTTTATCATCTCCTTTAACAATGGCATCCTACGGTGACGTTATTAATGTCCCGACGTACGAGTCTGTTAACAGCAAGTTCCACCGAAAA

Protein sequence

NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEPSKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTAPRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFEVPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSIFNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVPTYESVNSKFHRK
Homology
BLAST of MS013160 vs. NCBI nr
Match: XP_022153610.1 (mitochondrial-processing peptidase subunit alpha-like [Momordica charantia])

HSP 1 Score: 946.8 bits (2446), Expect = 7.4e-272
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP
Sbjct: 14  NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE
Sbjct: 254 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNSKFHRK
Sbjct: 494 TYESVNSKFHRK 505

BLAST of MS013160 vs. NCBI nr
Match: KAA0050857.1 (mitochondrial-processing peptidase subunit alpha-like [Cucumis melo var. makuwa] >TYK08495.1 mitochondrial-processing peptidase subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 897.5 bits (2318), Expect = 5.1e-257
Identity = 459/492 (93.29%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATSSAVA RPTSGGFFSWLTGERSSSLPP+E+PLSGI LPPPLPD+VEP
Sbjct: 14  NHAANLGASRFATSSAVATRPTSGGFFSWLTGERSSSLPPMEIPLSGINLPPPLPDHVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTYVPEMVELLVDCVRNP +LDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGV+HEELLSIAEPLLSDLPKVP  K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY  T S+FVAKAVDIAASELISIATPGQVS+VQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNS FHRK
Sbjct: 494 TYESVNSLFHRK 505

BLAST of MS013160 vs. NCBI nr
Match: XP_038876934.1 (mitochondrial-processing peptidase subunit alpha-like [Benincasa hispida])

HSP 1 Score: 896.0 bits (2314), Expect = 1.5e-256
Identity = 460/492 (93.50%), Postives = 478/492 (97.15%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NH ANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLE PLSGITLPPPLPD+VEP
Sbjct: 14  NHTANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLETPLSGITLPPPLPDHVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYL+ GSIYETP+SSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLNSGSIYETPVSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTYVPEMVELLVDCVRNP +LDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+GEL+NNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKLKAEIGELANNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGVEHEELLSIAEPLLSDLPKVP+ K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVEHEELLSIAEPLLSDLPKVPNVKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY ST S+FVA+AVDIA SELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYASTGSNFVAQAVDIATSELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNS+FHRK
Sbjct: 494 TYESVNSRFHRK 505

BLAST of MS013160 vs. NCBI nr
Match: XP_023527682.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 896.0 bits (2314), Expect = 1.5e-256
Identity = 460/490 (93.88%), Postives = 474/490 (96.73%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPLE PLSGITLPPPLPDYVEP
Sbjct: 14  NHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSGITLPPPLPDYVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETP+SSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTY PEMVELLVDCVRNP +LDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVRNPVFLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDGTILEEFIA+NYTA
Sbjct: 194 QKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDGTILEEFIADNYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGVEHEELLSIAEPLLSDLPKVPS K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY ST SDFVA AVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFH 491
           TYESVN +FH
Sbjct: 494 TYESVNRRFH 503

BLAST of MS013160 vs. NCBI nr
Match: XP_022980477.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita maxima])

HSP 1 Score: 896.0 bits (2314), Expect = 1.5e-256
Identity = 460/490 (93.88%), Postives = 475/490 (96.94%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPLE PLSGITLPPPLPDYVEP
Sbjct: 14  NHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSGITLPPPLPDYVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETP+SSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTY PEMVELLVDCVRNP +LDWEI+EEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVRNPVFLDWEIDEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDGTILEEFIA+NYTA
Sbjct: 194 QKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDGTILEEFIADNYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGVEHEELLSIAEPLLSDLPKVPS K PESAYVGGDYRRQ+DSGSTH ALAFE
Sbjct: 254 PRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQADSGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY ST SDFVA AVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFH 491
           TYESVN +FH
Sbjct: 494 TYESVNRRFH 503

BLAST of MS013160 vs. ExPASy Swiss-Prot
Match: P29677 (Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN=MPP PE=1 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.5e-198
Identity = 335/484 (69.21%), Postives = 419/484 (86.57%), Query Frame = 0

Query: 9   SRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEPSKTKITAL 68
           +RF++S+AVA +P SGG FSW+TG+ SSS+ PL+ PL+ + L PPLPDYVEP+KT+IT L
Sbjct: 22  TRFSSSAAVATKP-SGGLFSWITGDTSSSVTPLDFPLNDVKLSPPLPDYVEPAKTQITTL 81

Query: 69  PNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHLRIVREV 128
            NG+++ASE S NPAAS+G+Y+DCGSIYETP S GA+HLLER+AFKST NRSHLRIVRE+
Sbjct: 82  ANGLKVASEASVNPAASIGLYVDCGSIYETPASYGATHLLERMAFKSTLNRSHLRIVREI 141

Query: 129 EAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQKMRAELG 188
           EAIGGN  ASASRE M YT DALKTYVP+MVE+L DCVRNPA+LDWE+ E+L+K++AE+ 
Sbjct: 142 EAIGGNVTASASREHMIYTYDALKTYVPQMVEMLADCVRNPAFLDWEVKEQLEKVKAEIS 201

Query: 189 ELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTAPRIVLAAS 248
           E S NPQ LLLEA+HSAGY+G   N L+A E  ++RL+ T+LEEF+AENYTAPR+VLAAS
Sbjct: 202 EYSKNPQHLLLEAVHSAGYAGPYGNSLMATEATINRLNSTVLEEFVAENYTAPRMVLAAS 261

Query: 249 GVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFEVPGGWLKE 308
           GVEHEE L +AEPLLSDLPKV + + P+  YVGGDYR Q+D+  THFALAFEVPGGW+ E
Sbjct: 262 GVEHEEFLKVAEPLLSDLPKVATIEEPKPVYVGGDYRCQADAEMTHFALAFEVPGGWMSE 321

Query: 309 KDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSIFNNTGLFG 368
           K+++ LTVLQMLMGGGGSFSAGGPGKGM+SRLYLRVLN+Y QI +FSAF+SI+NNTGLFG
Sbjct: 322 KESMTLTVLQMLMGGGGSFSAGGPGKGMYSRLYLRVLNQYPQIHAFSAFSSIYNNTGLFG 381

Query: 369 IYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLESRMIVSEDI 428
           I G+TSSDF  +AVD+A  ELI++A P +V +VQLNRAK++TKSA+LMNLESRM+ SEDI
Sbjct: 382 IQGTTSSDFGPQAVDVAVKELIAVANPSEVDQVQLNRAKQATKSAILMNLESRMVASEDI 441

Query: 429 GRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVPTYESVNSK 488
           GRQ+LTYGER PVEHF+KA+D+++  DI ++ QK++SSPLTMASYGDV+++P+Y++V+S+
Sbjct: 442 GRQLLTYGERNPVEHFLKAIDAVSAKDIASVVQKLISSPLTMASYGDVLSLPSYDAVSSR 501

Query: 489 FHRK 493
           F  K
Sbjct: 502 FRSK 504

BLAST of MS013160 vs. ExPASy Swiss-Prot
Match: O04308 (Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha2 PE=1 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.6e-187
Identity = 328/489 (67.08%), Postives = 406/489 (83.03%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NH  N  ASR+A+SSAVA   TS    SWL+G  SSSLP + +PL+G++LPPPL D+VEP
Sbjct: 17  NH--NFRASRYASSSAVA---TSSSSSSWLSGGYSSSLPSMNIPLAGVSLPPPLSDHVEP 76

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SK K T LPNG+ IA+E S NPAAS+G+Y+DCGSIYETP   GA+HLLER+AFKST NRS
Sbjct: 77  SKLKTTTLPNGLTIATEMSPNPAASIGLYVDCGSIYETPQFRGATHLLERMAFKSTLNRS 136

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           H R+VRE+EAIGGNT ASASREQMGYT+DALKTYVPEMVE+L+D VRNPA+LDWE+NEEL
Sbjct: 137 HFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVLIDSVRNPAFLDWEVNEEL 196

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           +K++ E+GE + NP G LLEA+HSAGYSGALANPL APE A++ L G +LE F+ ENYTA
Sbjct: 197 RKVKVEIGEFATNPMGFLLEAVHSAGYSGALANPLYAPESAITGLTGEVLENFVFENYTA 256

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
            R+VLAASGV+HEELL + EPLLSDLP VP    P+S YVGGD+R+ +   +THFALAFE
Sbjct: 257 SRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQHTGGEATHFALAFE 316

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VP GW  EK+AI+ TVLQMLMGGGGSFSAGGPGKGMHS LYLR+LN++QQ QS +AF S+
Sbjct: 317 VP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWLYLRLLNQHQQFQSCTAFTSV 376

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIYG TS +F ++ +++ ASE+ ++A  G+V++  L+RAK +TKSA+LMNLES
Sbjct: 377 FNNTGLFGIYGCTSPEFASQGIELVASEMNAVA-DGKVNQKHLDRAKAATKSAILMNLES 436

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMI +EDIGRQILTYGERKPV+ F+K VD +TL DI + + KV++ PLTMA++GDV+NVP
Sbjct: 437 RMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIADFTSKVITKPLTMATFGDVLNVP 496

Query: 481 TYESVNSKF 490
           +Y+SV+ +F
Sbjct: 497 SYDSVSKRF 498

BLAST of MS013160 vs. ExPASy Swiss-Prot
Match: Q9ZU25 (Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha1 PE=1 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.2e-185
Identity = 326/486 (67.08%), Postives = 403/486 (82.92%), Query Frame = 0

Query: 5   NLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEPSKT 64
           +L  +R+A+SSAVA   +S   + SWL+G   ++L  L+MPL G++LPPPL D VEPSK 
Sbjct: 19  SLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKL 78

Query: 65  KITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHLR 124
           +IT LPNG++IASET+ NPAAS+G+Y+DCGSIYE P   GA+HLLER+AFKST NR+H R
Sbjct: 79  QITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGATHLLERMAFKSTLNRTHFR 138

Query: 125 IVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQKM 184
           +VRE+EAIGGNT ASASREQM YT+DALKTYVPEMVE+L+D VRNPA+LDWE+NEEL+KM
Sbjct: 139 LVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDSVRNPAFLDWEVNEELRKM 198

Query: 185 RAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTAPRI 244
           + E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL+G +LEEF+ EN+TA R+
Sbjct: 199 KVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRLNGELLEEFMTENFTAARM 258

Query: 245 VLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFEVPG 304
           VLAASGVEHEELL +AEPL SDLP VP   AP+S YVGGD+R+ +   +THFA+AFEVP 
Sbjct: 259 VLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQHTGGEATHFAVAFEVP- 318

Query: 305 GWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSIFNN 364
           GW  EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVLNEYQ++QS +AF SIFN+
Sbjct: 319 GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVLNEYQEVQSCTAFTSIFND 378

Query: 365 TGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLESRMI 424
           TGLFGIYG +S  F AKA+++AA EL  +A  G+V++  L+RAK +TKSAVLMNLESRMI
Sbjct: 379 TGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDRAKAATKSAVLMNLESRMI 438

Query: 425 VSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVPTYE 484
            +EDIGRQILTYGERKPV+ F+K+VD +TL DI + + KV+S PLTM S+GDV+ VP+Y+
Sbjct: 439 AAEDIGRQILTYGERKPVDQFLKSVDQLTLKDIADFTSKVISKPLTMGSFGDVLAVPSYD 498

Query: 485 SVNSKF 490
           +++SKF
Sbjct: 499 TISSKF 502

BLAST of MS013160 vs. ExPASy Swiss-Prot
Match: P97997 (Mitochondrial-processing peptidase subunit alpha OS=Blastocladiella emersonii OX=4808 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.0e-63
Identity = 145/428 (33.88%), Postives = 235/428 (54.91%), Query Frame = 0

Query: 63  TKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHL 122
           T +T LP+G+R+A+  S++  A+VG+Y+D G IYET +  G SH +  LAFKST   +  
Sbjct: 15  TCMTRLPSGIRVATAPSNSHFAAVGVYVDAGPIYETSIDRGVSHFVSSLAFKSTHGATES 74

Query: 123 RIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQK 182
           ++++ +  +GGN   +A+RE + Y    L   +P  V+LL D    PA  + EI E    
Sbjct: 75  QVLKTMAGLGGNLFCTATRESILYQGSVLHHDLPRTVQLLADTTLRPALTEEEIAERRAT 134

Query: 183 MRAELGELSNNPQGLLLEAIHSAGYSG-ALANPLVAPEFALSRLDGTILEEFIAENYTAP 242
           +  E  +L + P   + E +H+  + G  L N +         +    + E+ A      
Sbjct: 135 IAFEAEDLHSRPDAFIGEMMHAVAFGGRGLGNSIFCEPQRARNMTSDTIREYFATYLHPS 194

Query: 243 RIVLAASGVEHEELLS-IAEPLLSDLPKVPSSKAP---ESAYVGGDYR----------RQ 302
           R+V+A +GV H EL+  +++  +    + PSS      E+AYVGG ++            
Sbjct: 195 RMVVAGTGVAHAELVDLVSKAFVPSSTRAPSSVTHSDIETAYVGGSHQLVIPKPPPTHPN 254

Query: 303 SDSGSTHFALAFEVPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 362
            +   TH  +AF VP       D   ++ LQ+LMGGGG+FSAGGPGKGM+SRLY  VLN 
Sbjct: 255 YEQTLTHVQVAFPVPP--FTHPDMFPVSTLQVLMGGGGAFSAGGPGKGMYSRLYTNVLNR 314

Query: 363 YQQIQSFSAFNSIFNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAK 422
           Y+ ++S +AF   +++T LFGI  S    F     ++ A E + +A    +S+ ++ RAK
Sbjct: 315 YRWMESCAAFQHAYSSTSLFGISASCVPSFNPHLCNVLAGEFVHMAR--NLSDEEVARAK 374

Query: 423 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSP 476
              KS++LMNLES++I  EDIGRQ+L   +R      +  + ++T +D+  +++ +++ P
Sbjct: 375 NQLKSSLLMNLESQVITVEDIGRQVLAQNQRLEPLELVNNISAVTRDDLVRVAEALVAKP 434

BLAST of MS013160 vs. ExPASy Swiss-Prot
Match: P23955 (Mitochondrial-processing peptidase subunit alpha OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=mpp PE=1 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 1.1e-60
Identity = 155/495 (31.31%), Postives = 243/495 (49.09%), Query Frame = 0

Query: 59  EPS-KTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTT 118
           EP+ +  IT L NGVR+ASE   +  + VG+Y+D GS YE     GASH+++RLAFKST+
Sbjct: 47  EPTERDNITTLSNGVRVASEDLPDAFSGVGVYIDAGSRYENDYVRGASHIMDRLAFKSTS 106

Query: 119 NRSHLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEIN 178
            R+   ++  VE +GGN   ++SRE M Y        +P  VEL+ + +R+P   D E+ 
Sbjct: 107 TRTADEMLETVEKLGGNIQCASSRESMMYQAATFNKAIPTAVELMAETIRDPKLTDEELE 166

Query: 179 EELQKMRAELGELSNNPQGLLLEAIHSAGY-SGALANPLVAPEFALSRLDGTILEEFIAE 238
            ++   + E+ E+ +  + +L E +H A +    L NPL+ P+  L  ++  +++ +   
Sbjct: 167 GQIMTAQYEVNEIWSKAELILPELVHMAAFKDNTLGNPLLCPKERLDYINRDVIQTYRDA 226

Query: 239 NYTAPRIVLAASGVEHEELLSIAEPLLSD------------------------------- 298
            Y   R+V+A +GV HE  + +AE    D                               
Sbjct: 227 FYRPERLVVAFAGVPHERAVKLAEKYFGDMKASDAPGLSRTGSETSVDSLVSESSEASSE 286

Query: 299 ---------------LPKVPSSKAPESA--------------------YVGGDYRRQSDS 358
                          L K+ S KA ++                     Y GG     S  
Sbjct: 287 SSSSSSDSSESSGGLLSKLFSPKAKKATPNPFLTRVPISTEDLTRPAHYTGGFLTLPSQP 346

Query: 359 GS--------THFALAFEVPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYL 418
                     TH  LAFE  G  + + D   L  LQ L+GGGGSFSAGGPGKGM+SRLY 
Sbjct: 347 PPLNPNLPTFTHIQLAFE--GLAISDDDIYALATLQTLLGGGGSFSAGGPGKGMYSRLYT 406

Query: 419 RVLNEYQQIQSFSAFNSIFNNTGLFGIYGSTSSDFVAKAVDIAASELISIATP---GQVS 475
            VLN++  ++S  AFN  + ++GLFGI  S         + +   EL ++ T      + 
Sbjct: 407 NVLNQHGWVESCVAFNHSYTDSGLFGIAASCYPGRTLPMLQVMCRELHALTTDHGYSALG 466

BLAST of MS013160 vs. ExPASy TrEMBL
Match: A0A6J1DH96 (Alpha-MPP OS=Momordica charantia OX=3673 GN=LOC111021075 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.6e-272
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP
Sbjct: 14  NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE
Sbjct: 254 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNSKFHRK
Sbjct: 494 TYESVNSKFHRK 505

BLAST of MS013160 vs. ExPASy TrEMBL
Match: A0A5A7U8Y8 (Alpha-MPP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00220 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.5e-257
Identity = 459/492 (93.29%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATSSAVA RPTSGGFFSWLTGERSSSLPP+E+PLSGI LPPPLPD+VEP
Sbjct: 14  NHAANLGASRFATSSAVATRPTSGGFFSWLTGERSSSLPPMEIPLSGINLPPPLPDHVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTYVPEMVELLVDCVRNP +LDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGV+HEELLSIAEPLLSDLPKVP  K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY  T S+FVAKAVDIAASELISIATPGQVS+VQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNS FHRK
Sbjct: 494 TYESVNSLFHRK 505

BLAST of MS013160 vs. ExPASy TrEMBL
Match: A0A6J1IRH2 (Alpha-MPP OS=Cucurbita maxima OX=3661 GN=LOC111479857 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.2e-257
Identity = 460/490 (93.88%), Postives = 475/490 (96.94%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPLE PLSGITLPPPLPDYVEP
Sbjct: 14  NHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSGITLPPPLPDYVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETP+SSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTY PEMVELLVDCVRNP +LDWEI+EEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVRNPVFLDWEIDEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDGTILEEFIA+NYTA
Sbjct: 194 QKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDGTILEEFIADNYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGVEHEELLSIAEPLLSDLPKVPS K PESAYVGGDYRRQ+DSGSTH ALAFE
Sbjct: 254 PRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQADSGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY ST SDFVA AVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFH 491
           TYESVN +FH
Sbjct: 494 TYESVNRRFH 503

BLAST of MS013160 vs. ExPASy TrEMBL
Match: A0A1S3BIC6 (Alpha-MPP OS=Cucumis melo OX=3656 GN=LOC103489980 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 457/492 (92.89%), Postives = 476/492 (96.75%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATS+AVA RPTSGGFFSWLTGERSSSLPP+E+PLSGI LPPPLPD+VEP
Sbjct: 14  NHAANLGASRFATSTAVATRPTSGGFFSWLTGERSSSLPPMEIPLSGINLPPPLPDHVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNGVRIASETSSNPAAS+GIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTYVPEMVELLVDCVRNP +LDWEINEEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGV+HEELLSIAEPLLSDLPKVP  K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIY  T S+FVAKAVDIAASELISIATPGQVS+VQLNRAKESTKSAVLMNLES
Sbjct: 374 FNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVN  FHRK
Sbjct: 494 TYESVNGLFHRK 505

BLAST of MS013160 vs. ExPASy TrEMBL
Match: A0A6J1GAE8 (Alpha-MPP OS=Cucurbita moschata OX=3662 GN=LOC111452402 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.7e-256
Identity = 460/492 (93.50%), Postives = 476/492 (96.75%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLE PLS ITLPPPLPD+VEP
Sbjct: 14  NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLETPLSDITLPPPLPDHVEP 73

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SKTKIT LPNG RIASETSSNPAAS+GIYLDCGSIYETP SSGASHLLERLAFKSTTNRS
Sbjct: 74  SKTKITTLPNGARIASETSSNPAASIGIYLDCGSIYETPSSSGASHLLERLAFKSTTNRS 133

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           HLRIVREVEAIGGNTGASASREQMGYT DALKTYVPEMVELLVDCVRNP +LDWEI EEL
Sbjct: 134 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEIKEEL 193

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           QK++AE+GELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA
Sbjct: 194 QKLKAEIGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 253

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
           PR+VLAASGVEHEELLSIAEPLLSDLPKVPS K PESAYVGGDYRRQ+D GSTH ALAFE
Sbjct: 254 PRMVLAASGVEHEELLSIAEPLLSDLPKVPSLKVPESAYVGGDYRRQADCGSTHVALAFE 313

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VPGGW KEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI
Sbjct: 314 VPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 373

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FN+TGLFGIY ST S+FVA+AVDIAASELISIATPGQVS+VQLNRAKESTKSAVLMNLES
Sbjct: 374 FNDTGLFGIYASTGSNFVAQAVDIAASELISIATPGQVSQVQLNRAKESTKSAVLMNLES 433

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMIVSEDIGRQILTYGERKPVEHF+KAVDSITLNDITNI+QKV+SSPLTMASYGDVINVP
Sbjct: 434 RMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSPLTMASYGDVINVP 493

Query: 481 TYESVNSKFHRK 493
           TYESVNS+FH K
Sbjct: 494 TYESVNSRFHSK 505

BLAST of MS013160 vs. TAIR 10
Match: AT3G16480.1 (mitochondrial processing peptidase alpha subunit )

HSP 1 Score: 656.4 bits (1692), Expect = 1.8e-188
Identity = 328/489 (67.08%), Postives = 406/489 (83.03%), Query Frame = 0

Query: 1   NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEP 60
           NH  N  ASR+A+SSAVA   TS    SWL+G  SSSLP + +PL+G++LPPPL D+VEP
Sbjct: 17  NH--NFRASRYASSSAVA---TSSSSSSWLSGGYSSSLPSMNIPLAGVSLPPPLSDHVEP 76

Query: 61  SKTKITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 120
           SK K T LPNG+ IA+E S NPAAS+G+Y+DCGSIYETP   GA+HLLER+AFKST NRS
Sbjct: 77  SKLKTTTLPNGLTIATEMSPNPAASIGLYVDCGSIYETPQFRGATHLLERMAFKSTLNRS 136

Query: 121 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 180
           H R+VRE+EAIGGNT ASASREQMGYT+DALKTYVPEMVE+L+D VRNPA+LDWE+NEEL
Sbjct: 137 HFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVLIDSVRNPAFLDWEVNEEL 196

Query: 181 QKMRAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTA 240
           +K++ E+GE + NP G LLEA+HSAGYSGALANPL APE A++ L G +LE F+ ENYTA
Sbjct: 197 RKVKVEIGEFATNPMGFLLEAVHSAGYSGALANPLYAPESAITGLTGEVLENFVFENYTA 256

Query: 241 PRIVLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFE 300
            R+VLAASGV+HEELL + EPLLSDLP VP    P+S YVGGD+R+ +   +THFALAFE
Sbjct: 257 SRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQHTGGEATHFALAFE 316

Query: 301 VPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSI 360
           VP GW  EK+AI+ TVLQMLMGGGGSFSAGGPGKGMHS LYLR+LN++QQ QS +AF S+
Sbjct: 317 VP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWLYLRLLNQHQQFQSCTAFTSV 376

Query: 361 FNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLES 420
           FNNTGLFGIYG TS +F ++ +++ ASE+ ++A  G+V++  L+RAK +TKSA+LMNLES
Sbjct: 377 FNNTGLFGIYGCTSPEFASQGIELVASEMNAVA-DGKVNQKHLDRAKAATKSAILMNLES 436

Query: 421 RMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVP 480
           RMI +EDIGRQILTYGERKPV+ F+K VD +TL DI + + KV++ PLTMA++GDV+NVP
Sbjct: 437 RMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIADFTSKVITKPLTMATFGDVLNVP 496

Query: 481 TYESVNSKF 490
           +Y+SV+ +F
Sbjct: 497 SYDSVSKRF 498

BLAST of MS013160 vs. TAIR 10
Match: AT1G51980.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.0e-186
Identity = 326/486 (67.08%), Postives = 403/486 (82.92%), Query Frame = 0

Query: 5   NLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEPSKT 64
           +L  +R+A+SSAVA   +S   + SWL+G   ++L  L+MPL G++LPPPL D VEPSK 
Sbjct: 19  SLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKL 78

Query: 65  KITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHLR 124
           +IT LPNG++IASET+ NPAAS+G+Y+DCGSIYE P   GA+HLLER+AFKST NR+H R
Sbjct: 79  QITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGATHLLERMAFKSTLNRTHFR 138

Query: 125 IVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQKM 184
           +VRE+EAIGGNT ASASREQM YT+DALKTYVPEMVE+L+D VRNPA+LDWE+NEEL+KM
Sbjct: 139 LVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDSVRNPAFLDWEVNEELRKM 198

Query: 185 RAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTAPRI 244
           + E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL+G +LEEF+ EN+TA R+
Sbjct: 199 KVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRLNGELLEEFMTENFTAARM 258

Query: 245 VLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFEVPG 304
           VLAASGVEHEELL +AEPL SDLP VP   AP+S YVGGD+R+ +   +THFA+AFEVP 
Sbjct: 259 VLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQHTGGEATHFAVAFEVP- 318

Query: 305 GWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSIFNN 364
           GW  EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVLNEYQ++QS +AF SIFN+
Sbjct: 319 GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVLNEYQEVQSCTAFTSIFND 378

Query: 365 TGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLESRMI 424
           TGLFGIYG +S  F AKA+++AA EL  +A  G+V++  L+RAK +TKSAVLMNLESRMI
Sbjct: 379 TGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDRAKAATKSAVLMNLESRMI 438

Query: 425 VSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVLSSPLTMASYGDVINVPTYE 484
            +EDIGRQILTYGERKPV+ F+K+VD +TL DI + + KV+S PLTM S+GDV+ VP+Y+
Sbjct: 439 AAEDIGRQILTYGERKPVDQFLKSVDQLTLKDIADFTSKVISKPLTMGSFGDVLAVPSYD 498

Query: 485 SVNSKF 490
           +++SKF
Sbjct: 499 TISSKF 502

BLAST of MS013160 vs. TAIR 10
Match: AT1G51980.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 583.9 bits (1504), Expect = 1.2e-166
Identity = 297/434 (68.43%), Postives = 360/434 (82.95%), Query Frame = 0

Query: 5   NLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLEMPLSGITLPPPLPDYVEPSKT 64
           +L  +R+A+SSAVA   +S   + SWL+G   ++L  L+MPL G++LPPPL D VEPSK 
Sbjct: 19  SLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKL 78

Query: 65  KITALPNGVRIASETSSNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRSHLR 124
           +IT LPNG++IASET+ NPAAS+G+Y+DCGSIYE P   GA+HLLER+AFKST NR+H R
Sbjct: 79  QITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGATHLLERMAFKSTLNRTHFR 138

Query: 125 IVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEELQKM 184
           +VRE+EAIGGNT ASASREQM YT+DALKTYVPEMVE+L+D VRNPA+LDWE+NEEL+KM
Sbjct: 139 LVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDSVRNPAFLDWEVNEELRKM 198

Query: 185 RAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDGTILEEFIAENYTAPRI 244
           + E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL+G +LEEF+ EN+TA R+
Sbjct: 199 KVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRLNGELLEEFMTENFTAARM 258

Query: 245 VLAASGVEHEELLSIAEPLLSDLPKVPSSKAPESAYVGGDYRRQSDSGSTHFALAFEVPG 304
           VLAASGVEHEELL +AEPL SDLP VP   AP+S YVGGD+R+ +   +THFA+AFEVP 
Sbjct: 259 VLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQHTGGEATHFAVAFEVP- 318

Query: 305 GWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQIQSFSAFNSIFNN 364
           GW  EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVLNEYQ++QS +AF SIFN+
Sbjct: 319 GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVLNEYQEVQSCTAFTSIFND 378

Query: 365 TGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTKSAVLMNLESRMI 424
           TGLFGIYG +S  F AKA+++AA EL  +A  G+V++  L+RAK +TKSAVLMNLESRMI
Sbjct: 379 TGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDRAKAATKSAVLMNLESRMI 438

Query: 425 VSEDIGRQILTYGE 438
            +EDIGRQILTYGE
Sbjct: 439 AAEDIGRQILTYGE 450

BLAST of MS013160 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 196.8 bits (499), Expect = 4.0e-50
Identity = 131/442 (29.64%), Postives = 242/442 (54.75%), Query Frame = 0

Query: 62  KTKITALPNGVRIASETS-SNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 121
           +T++T LPNG+R+A+E++ S   A+VG+++D GS +E+  ++G +H LE + FK T  R+
Sbjct: 96  ETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRT 155

Query: 122 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 181
              +  E+E IGG+  A  SREQ  Y    L + V + +++L D ++N  + +  IN E 
Sbjct: 156 VRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADILQNSKFEEQRINRER 215

Query: 182 QKMRAELGELSNNPQGLLLEAIHSAGYS-GALANPLVAPEFALSRLDGTILEEFIAENYT 241
             +  E+ E+      ++L+ +H+  +    L   ++ P   +  +    L+ +I  +YT
Sbjct: 216 DVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYT 275

Query: 242 APRIVLAASG-VEHEELLSIAEPLLSDLPKVPSSKA-----PESAYVGGDYRR-QSDSGS 301
           A R+V+AA+G V+HEE++   + L + L   P++ +       +++ G + R    D   
Sbjct: 276 ASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPL 335

Query: 302 THFALAFEVPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV-LNEYQQI 361
             FA+AFE  G    + D++ L V+Q ++G     + GG GK + S L  RV +NE    
Sbjct: 336 AQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--A 395

Query: 362 QSFSAFNSIFNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTK 421
           +S  AFN+ + +TGLFG+Y    +D +         E+  +A   +VS+  + RA+   K
Sbjct: 396 ESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAY--RVSDADVTRARNQLK 455

Query: 422 SAVLMNLESRMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQK-VLSSPLTM 481
           S++L++++    ++EDIGRQ+LTYG R P       +D++  + +  ++ K +    + +
Sbjct: 456 SSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYIYDKDIAI 515

Query: 482 ASYGDVINVPTYESVNSKFHRK 493
           ++ G + ++P Y    +KF R+
Sbjct: 516 SAIGPIQDLPDY----NKFRRR 525

BLAST of MS013160 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-48
Identity = 125/413 (30.27%), Postives = 227/413 (54.96%), Query Frame = 0

Query: 62  KTKITALPNGVRIASETS-SNPAASVGIYLDCGSIYETPLSSGASHLLERLAFKSTTNRS 121
           +T++T LPNG+R+A+E++ S   A+VG+++D GS +E+  ++G +H LE + FK T  R+
Sbjct: 96  ETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRT 155

Query: 122 HLRIVREVEAIGGNTGASASREQMGYTLDALKTYVPEMVELLVDCVRNPAYLDWEINEEL 181
              +  E+E IGG+  A  SREQ  Y    L + V + +++L D ++N  + +  IN E 
Sbjct: 156 VRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADILQNSKFEEQRINRER 215

Query: 182 QKMRAELGELSNNPQGLLLEAIHSAGYS-GALANPLVAPEFALSRLDGTILEEFIAENYT 241
             +  E+ E+      ++L+ +H+  +    L   ++ P   +  +    L+ +I  +YT
Sbjct: 216 DVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYT 275

Query: 242 APRIVLAASG-VEHEELLSIAEPLLSDLPKVPSSKA-----PESAYVGGDYRR-QSDSGS 301
           A R+V+AA+G V+HEE++   + L + L   P++ +       +++ G + R    D   
Sbjct: 276 ASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPL 335

Query: 302 THFALAFEVPGGWLKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV-LNEYQQI 361
             FA+AFE  G    + D++ L V+Q ++G     + GG GK + S L  RV +NE    
Sbjct: 336 AQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--A 395

Query: 362 QSFSAFNSIFNNTGLFGIYGSTSSDFVAKAVDIAASELISIATPGQVSEVQLNRAKESTK 421
           +S  AFN+ + +TGLFG+Y    +D +         E+  +A   +VS+  + RA+   K
Sbjct: 396 ESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAY--RVSDADVTRARNQLK 455

Query: 422 SAVLMNLESRMIVSEDIGRQILTYGERKPVEHFIKAVDSITLNDITNISQKVL 465
           S++L++++    ++EDIGRQ+LTYG R P       +D++  + +  ++ K +
Sbjct: 456 SSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYI 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153610.17.4e-272100.00mitochondrial-processing peptidase subunit alpha-like [Momordica charantia][more]
KAA0050857.15.1e-25793.29mitochondrial-processing peptidase subunit alpha-like [Cucumis melo var. makuwa]... [more]
XP_038876934.11.5e-25693.50mitochondrial-processing peptidase subunit alpha-like [Benincasa hispida][more]
XP_023527682.11.5e-25693.88mitochondrial-processing peptidase subunit alpha-like [Cucurbita pepo subsp. pep... [more]
XP_022980477.11.5e-25693.88mitochondrial-processing peptidase subunit alpha-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P296772.5e-19869.21Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN... [more]
O043082.6e-18767.08Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitoc... [more]
Q9ZU254.2e-18567.08Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Ar... [more]
P979974.0e-6333.88Mitochondrial-processing peptidase subunit alpha OS=Blastocladiella emersonii OX... [more]
P239551.1e-6031.31Mitochondrial-processing peptidase subunit alpha OS=Neurospora crassa (strain AT... [more]
Match NameE-valueIdentityDescription
A0A6J1DH963.6e-272100.00Alpha-MPP OS=Momordica charantia OX=3673 GN=LOC111021075 PE=3 SV=1[more]
A0A5A7U8Y82.5e-25793.29Alpha-MPP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00220 PE=3... [more]
A0A6J1IRH27.2e-25793.88Alpha-MPP OS=Cucurbita maxima OX=3661 GN=LOC111479857 PE=3 SV=1[more]
A0A1S3BIC61.6e-25692.89Alpha-MPP OS=Cucumis melo OX=3656 GN=LOC103489980 PE=3 SV=1[more]
A0A6J1GAE82.7e-25693.50Alpha-MPP OS=Cucurbita moschata OX=3662 GN=LOC111452402 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G16480.11.8e-18867.08mitochondrial processing peptidase alpha subunit [more]
AT1G51980.13.0e-18667.08Insulinase (Peptidase family M16) protein [more]
AT1G51980.21.2e-16668.43Insulinase (Peptidase family M16) protein [more]
AT3G02090.14.0e-5029.64Insulinase (Peptidase family M16) protein [more]
AT3G02090.22.2e-4830.27Insulinase (Peptidase family M16) protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011765Peptidase M16, N-terminalPFAMPF00675Peptidase_M16coord: 73..218
e-value: 1.8E-35
score: 122.2
IPR007863Peptidase M16, C-terminalPFAMPF05193Peptidase_M16_Ccoord: 230..408
e-value: 3.0E-31
score: 108.9
NoneNo IPR availableGENE3D3.30.830.10coord: 55..265
e-value: 2.9E-45
score: 156.5
NoneNo IPR availableGENE3D3.30.830.10coord: 277..489
e-value: 4.4E-63
score: 214.7
NoneNo IPR availablePANTHERPTHR11851METALLOPROTEASEcoord: 5..491
IPR037715Mitochondrial-processing peptidase subunit alphaPANTHERPTHR11851:SF193MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA-LIKEcoord: 5..491
IPR001431Peptidase M16, zinc-binding sitePROSITEPS00143INSULINASEcoord: 93..116
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 279..483
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 62..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013160.1MS013160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006627 protein processing involved in protein targeting to mitochondrion
biological_process GO:0006508 proteolysis
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity