MS013139 (gene) Bitter gourd (TR) v1

BLAST of MS013139 vs. NCBI nr
Match: XP_022153556.1 (VHS domain-containing protein At3g16270 [Momordica charantia])

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 667/691 (96.53%), Postives = 669/691 (96.82%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFI KRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFIWKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG
Sbjct: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNFTQGHSSRKNGASS RGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT
Sbjct: 181 LSNFTQGHSSRKNGASSHRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAA  
Sbjct: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAAKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALALSYALESKLTSPSWQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS
Sbjct: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
           QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL
Sbjct: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMNVDSNHVTNENKKPVSE+KNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED
Sbjct: 481 FSGMNVDSNHVTNENKKPVSEKKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALKSKDK DSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL
Sbjct: 541 ALKSKDKEDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP
Sbjct: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS
Sbjct: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 691

BLAST of MS013139 vs. NCBI nr
Match: XP_038890584.1 (protein MODIFIED TRANSPORT TO THE VACUOLE 1 [Benincasa hispida])

HSP 1 Score: 1073.2 bits (2774), Expect = 1.1e-309
Identity = 578/693 (83.41%), Postives = 612/693 (88.31%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQR+SVAVRQLFHYKGQ DPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AIS+IF+EEDNKPAPSENLN RIQGFGNSNYE PSEDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHDAISSIFAEEDNKPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSN  QGHSSRKNG SSQ+G NLQRSLTTE+EYDNRYEPVEYGRET   LGTS+STTSGT
Sbjct: 181 LSNLAQGHSSRKNGTSSQQGFNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRV      NG+  SG SESKTREERLLD+IAT+GGVRLQPTRDAIQAF VEA   
Sbjct: 241 WNQDSRV-----INGNPGSGTSESKTREERLLDSIATAGGVRLQPTRDAIQAFLVEAVKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILES+VR++DDDHFSIVTSYFSEN DAVIGCSES
Sbjct: 301 DALALSNALETKLTSPSWQVRFKALCILESMVRRNDDDHFSIVTSYFSENPDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLREKASKV+PLLDGGKGVP MN+SEKS+PSNTSST+QMPDLIDTSDA DYG T  S
Sbjct: 361 PQASLREKASKVMPLLDGGKGVPFMNESEKSVPSNTSSTVQMPDLIDTSDAGDYGETNKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETRE++DDL
Sbjct: 421 LEVENL-------SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETRENSDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN D+N VTNENKKP SEQKN+ G FDIFGSSSEPA+QEHARKDVNDL+SGLS+HED
Sbjct: 481 FSGMNFDNNQVTNENKKPASEQKNETGVFDIFGSSSEPAVQEHARKDVNDLISGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
            LKSKDKG+SKDSLS S FSVSSQPNHQ Q+PQDSLNGMYSSPMVG NMNATF PGM YL
Sbjct: 541 TLKSKDKGNSKDSLSESLFSVSSQPNHQNQVPQDSLNGMYSSPMVGTNMNATFFPGMTYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAV-NGG-TAP 660
           P STMF+ AFSSQPM YAPTGNFF QQQLLSAMSNYQQFGNPNLQSS GG V NGG ++P
Sbjct: 601 PSSTMFNPAFSSQPMAYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSAGGGVGNGGYSSP 660

Query: 661 LPDIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           LPDIF PNL  Q+P+S+MN SKKE+TRAFDFIS
Sbjct: 661 LPDIFQPNLAAQSPTSVMNGSKKEDTRAFDFIS 678

BLAST of MS013139 vs. NCBI nr
Match: XP_022979433.1 (VHS domain-containing protein At3g16270-like [Cucurbita maxima])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.3e-308
Identity = 575/691 (83.21%), Postives = 605/691 (87.55%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQ DPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AISAIF+EEDNKPAPSENLN RIQGFGNSNYE P EDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RGPNLQRSLTTE+EYDNRYEPVEYGRET   LGTS+ST SGT
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTISGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NG+ SSG S SKTREERLL+TIAT+GGVRLQPTRDAIQAF VEAA  
Sbjct: 241 WNQDSRVS-----NGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAML 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR+  D+HFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALALSNALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLR+KASKV+PLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDL+DTSDA DYGGT  S
Sbjct: 361 PQASLRDKASKVMPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SS PLVDDLFG GLNTVTSTSELKNDDDPFSDV FHTTETRE+ DD+
Sbjct: 421 LEVENL-------SSVPLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDI 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN ++N VT+ENKKP SEQKN+PG FDIFGSSSEPA+QEHARKDV DLMSGLS+HED
Sbjct: 481 FSGMNFENNQVTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALK+KDKGDSKDSLS S FSVSSQPNHQ Q+P DSL G YSSPMVG NMNATF PGM YL
Sbjct: 541 ALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           P   MF+ AFSSQPMGYAPTGNFF QQQLLSAMSNYQQFGNPNLQSSGGG     ++PLP
Sbjct: 601 PSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGY----SSPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIF PNL  Q+PSS+MNSSKKE+TRAFDFIS
Sbjct: 661 DIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 672

BLAST of MS013139 vs. NCBI nr
Match: XP_023526954.1 (VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1068.1 bits (2761), Expect = 3.1e-308
Identity = 575/691 (83.21%), Postives = 605/691 (87.55%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQ DPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AISAIF+EEDNKPAPSENLN RIQGFGNSNYE P EDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RGPNLQRSLTTE+ YDNRYEPVEYGRET   LGTS+STTSGT
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIAYDNRYEPVEYGRET---LGTSKSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NG+ SSG S SKTREERLL+TIAT+GGVRLQPTRDAIQAF VEAA  
Sbjct: 241 WNQDSRVS-----NGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR+  D+HFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALVLSHALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLR+KASKV+PLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDL+DTSDA DYGGT  S
Sbjct: 361 PQASLRDKASKVMPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SS PLVDDLFG GLNTVTSTSELKNDDDPFSDV FHTTETRE+ DDL
Sbjct: 421 LEVENL-------SSVPLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN +++ VT+ENKKP SEQKN+PG FDIFGSSSEPA+QEHARKDV DLMSGLS+HED
Sbjct: 481 FSGMNFENDQVTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALK+KDKGDSKDSLS S FSVSSQPNHQ Q+P DSL G YSSPMVG NMNATF PGM YL
Sbjct: 541 ALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           P   MF+ AFSSQPMGYAPTGNFF QQQLLSAMSNYQQFGNPNLQSSGGG     ++PLP
Sbjct: 601 PSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGY----SSPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIF PNL  Q+PSS+MNSSKKE+TRAFDFIS
Sbjct: 661 DIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 672

BLAST of MS013139 vs. NCBI nr
Match: XP_022955654.1 (VHS domain-containing protein At3g16270-like [Cucurbita moschata])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.4e-307
Identity = 574/691 (83.07%), Postives = 603/691 (87.26%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQ DPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AISAIF+EEDNKPAPSENLN RIQGFGNSNYE P EDKKSFL EVVGLGSASIK G
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKHG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RGPNLQRSLTTE+EYDNRYEPVEYGRET   LGTS+STTSGT
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NG+ SSG S SKTREERLL+TIAT+GGVRLQPTRDAIQAF VEAA  
Sbjct: 241 WNQDSRVS-----NGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR+  D+HFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALVLSHALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLR+KASKV+PLLDGGKGVP M DSEKSLPSNTSSTIQMPDL+DTSDA DYGGT  S
Sbjct: 361 PQASLRDKASKVMPLLDGGKGVPTMTDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SS PLVDDLFG GLNTVTSTSELKNDDDPFSDV FHTTETRE+ DDL
Sbjct: 421 LEVENL-------SSVPLVDDLFGVGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN ++N VT+ENKKP SEQKN+PG FDIFGSSSEPA+QEHARKDV DLMSGLS+HED
Sbjct: 481 FSGMNFENNQVTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALK+KDKGDSKDSLS S FSVSSQPNHQ Q+P DSL G YSSPMVG NMNAT+ PGM YL
Sbjct: 541 ALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATYFPGMPYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           P   MF+ AFSSQPMGYAPTGNFF QQQLLSAMSNYQQFGNPNLQSSGGG     ++PLP
Sbjct: 601 PSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGY----SSPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIF PNL  Q+PSSMMNSSKKE+TRAFDFIS
Sbjct: 661 DIFQPNLAAQSPSSMMNSSKKEDTRAFDFIS 672

BLAST of MS013139 vs. ExPASy Swiss-Prot
Match: Q9C5H4 (Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=MTV1 PE=1 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 6.0e-182
Identity = 388/709 (54.72%), Postives = 484/709 (68.27%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MD+SRRAVESYWRSRMID  TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL+
Sbjct: 1   MDTSRRAVESYWRSRMIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLD 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           +KSPIVKQKALRLIKYAVGKSG EFRREMQR+SVAVR LFHYKG  DPLKGDALNKAVR+
Sbjct: 61  NKSPIVKQKALRLIKYAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEED-NKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQ 180
           TAHE ISAIFSEE+  KPA  E++N RI+GFGN+N++VPS D KSFL EVVG+GSASIKQ
Sbjct: 121 TAHETISAIFSEENGTKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQ 180

Query: 181 GLSNFTQGHSSRK--NGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTT 240
           G+SNF QGH  +K  NG+SS RGPNL RSLT E E  +RY+PV+ G++  G+ GTS++TT
Sbjct: 181 GISNFAQGHLPKKNENGSSSYRGPNLHRSLTMENENFSRYDPVKLGKD--GNYGTSKNTT 240

Query: 241 SGTWNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEA 300
            G+W   S        + S +S   ESKTREE+LL+TI TSGGVRLQPTRDA+  F +EA
Sbjct: 241 GGSWGHAS-----GEASESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEA 300

Query: 301 ANF----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGC 360
           A                  ++QVR KALC+LE+I+RK +D++FSIV +YFSEN DA+  C
Sbjct: 301 AKMDAVALSIALDGKLHSPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRC 360

Query: 361 SESPQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGT 420
           +ESPQ+SLREKA+KV+ LL+GG+    M+ S+ ++     + + +PDLIDT D+DD    
Sbjct: 361 AESPQSSLREKANKVLSLLNGGQSSGLMSSSDNTV--KREAAVDLPDLIDTGDSDD--TL 420

Query: 421 KNSQEVENSKNISINPSSTPLV-DDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREH 480
            N   ++    ++   ++ PL+ DD FGD  +   S+SE K DDDPF+DVSFH  E +E 
Sbjct: 421 NNLNAIDTGSTVA---TAGPLMDDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKES 480

Query: 481 TDDLFSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLS 540
            DDLFSGM V         K       + P  FD+FGS+++   +    K++NDLM   S
Sbjct: 481 ADDLFSGMTVG-------EKSAAVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFS 540

Query: 541 VHEDALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPG 600
           + E+   S  KG S  +L    F++ S  +H  Q P++ + G+  S   G   N T LPG
Sbjct: 541 IDEN--NSNQKGSSSSTLPQDLFAMPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPG 600

Query: 601 --MAY-LPPSTMFSTAFSSQPMGYAPTGNFFA-QQQLLSAMSNYQQFGNPNLQSSGG--- 660
             M +  P   M + AF+SQP+ YA   +  A QQQ L  MSN+QQFGN N Q SG    
Sbjct: 601 GVMPFNFPQGMMMNPAFASQPLNYAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLS 660

Query: 661 -GAVNGGTAPLPDIFHPNLPTQAPSSMMNSSKKEETRAFDFISVSLVSS 682
            G   G  + LPDIF PN   QAP+S MN SKKE+TRAFDFIS  L S+
Sbjct: 661 MGTSGGNQSALPDIFQPNFGNQAPTSTMNGSKKEDTRAFDFISDHLTSA 683

BLAST of MS013139 vs. ExPASy Swiss-Prot
Match: Q3U3N6 (AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-06
Identity = 40/123 (32.52%), Postives = 65/123 (52.85%), Query Frame = 0

Query: 21  TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYAV 80
           TSD+    P Y  EEI ++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDSIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 81  GKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRDTAHEAISAIFSEEDNKPA 140
           G     F   ++R+S  +++   + G  DPL G++L + VR  A +  S +FS+   +P 
Sbjct: 84  GHGSSSFLLILRRNSALIQEATAFSGPPDPLHGNSLYQKVRAAAQDLGSTLFSDAVPQP- 142

Query: 141 PSE 142
           PS+
Sbjct: 144 PSQ 142

BLAST of MS013139 vs. ExPASy Swiss-Prot
Match: G3V8Y7 (AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-06
Identity = 45/147 (30.61%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 21  TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYAV 80
           TSD+D   P Y  EEI ++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDDIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 81  GKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRDTAHEAISAIFSEEDNKPA 140
                 F   ++R+S  +++   + G  DPL G++L + VR  A +  S +FS+   +P 
Sbjct: 84  SHGSSSFMLILRRNSALIQEATAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSDALPQP- 143

Query: 141 PSE--------------NLNSRIQGFG 152
           PS+                +S +QGFG
Sbjct: 144 PSQPPQILPPAGMGAQARPHSALQGFG 166

BLAST of MS013139 vs. ExPASy TrEMBL
Match: A0A6J1DKZ7 (VHS domain-containing protein At3g16270 OS=Momordica charantia OX=3673 GN=LOC111021032 PE=4 SV=1)

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 667/691 (96.53%), Postives = 669/691 (96.82%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFI KRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFIWKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG
Sbjct: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNFTQGHSSRKNGASS RGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT
Sbjct: 181 LSNFTQGHSSRKNGASSHRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAA  
Sbjct: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAAKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALALSYALESKLTSPSWQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS
Sbjct: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
           QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL
Sbjct: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMNVDSNHVTNENKKPVSE+KNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED
Sbjct: 481 FSGMNVDSNHVTNENKKPVSEKKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALKSKDK DSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL
Sbjct: 541 ALKSKDKEDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP
Sbjct: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS
Sbjct: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 691

BLAST of MS013139 vs. ExPASy TrEMBL
Match: A0A6J1IW71 (VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC111479156 PE=4 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 1.1e-308
Identity = 575/691 (83.21%), Postives = 605/691 (87.55%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQ DPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AISAIF+EEDNKPAPSENLN RIQGFGNSNYE P EDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RGPNLQRSLTTE+EYDNRYEPVEYGRET   LGTS+ST SGT
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTISGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NG+ SSG S SKTREERLL+TIAT+GGVRLQPTRDAIQAF VEAA  
Sbjct: 241 WNQDSRVS-----NGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAML 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR+  D+HFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALALSNALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLR+KASKV+PLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDL+DTSDA DYGGT  S
Sbjct: 361 PQASLRDKASKVMPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SS PLVDDLFG GLNTVTSTSELKNDDDPFSDV FHTTETRE+ DD+
Sbjct: 421 LEVENL-------SSVPLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDI 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN ++N VT+ENKKP SEQKN+PG FDIFGSSSEPA+QEHARKDV DLMSGLS+HED
Sbjct: 481 FSGMNFENNQVTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALK+KDKGDSKDSLS S FSVSSQPNHQ Q+P DSL G YSSPMVG NMNATF PGM YL
Sbjct: 541 ALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           P   MF+ AFSSQPMGYAPTGNFF QQQLLSAMSNYQQFGNPNLQSSGGG     ++PLP
Sbjct: 601 PSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGY----SSPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIF PNL  Q+PSS+MNSSKKE+TRAFDFIS
Sbjct: 661 DIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 672

BLAST of MS013139 vs. ExPASy TrEMBL
Match: A0A6J1GWW5 (VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LOC111457592 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.6e-307
Identity = 574/691 (83.07%), Postives = 603/691 (87.26%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQ DPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAH+AISAIF+EEDNKPAPSENLN RIQGFGNSNYE P EDKKSFL EVVGLGSASIK G
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKHG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RGPNLQRSLTTE+EYDNRYEPVEYGRET   LGTS+STTSGT
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NG+ SSG S SKTREERLL+TIAT+GGVRLQPTRDAIQAF VEAA  
Sbjct: 241 WNQDSRVS-----NGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR+  D+HFSIVTSYFSENQDAVIGCSES
Sbjct: 301 DALVLSHALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLR+KASKV+PLLDGGKGVP M DSEKSLPSNTSSTIQMPDL+DTSDA DYGGT  S
Sbjct: 361 PQASLRDKASKVMPLLDGGKGVPTMTDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SS PLVDDLFG GLNTVTSTSELKNDDDPFSDV FHTTETRE+ DDL
Sbjct: 421 LEVENL-------SSVPLVDDLFGVGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN ++N VT+ENKKP SEQKN+PG FDIFGSSSEPA+QEHARKDV DLMSGLS+HED
Sbjct: 481 FSGMNFENNQVTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
           ALK+KDKGDSKDSLS S FSVSSQPNHQ Q+P DSL G YSSPMVG NMNAT+ PGM YL
Sbjct: 541 ALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATYFPGMPYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGGTAPLP 660
           P   MF+ AFSSQPMGYAPTGNFF QQQLLSAMSNYQQFGNPNLQSSGGG     ++PLP
Sbjct: 601 PSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGY----SSPLP 660

Query: 661 DIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           DIF PNL  Q+PSSMMNSSKKE+TRAFDFIS
Sbjct: 661 DIFQPNLAAQSPSSMMNSSKKEDTRAFDFIS 672

BLAST of MS013139 vs. ExPASy TrEMBL
Match: A0A5A7UB67 (VHS domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00690 PE=4 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 8.1e-307
Identity = 575/704 (81.68%), Postives = 606/704 (86.08%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSP+VKQKALRLIKYAVGKSGVEFRREMQR+SVAVRQLFHYKGQ DPLKGDALNKAVRD
Sbjct: 61  HKSPVVKQKALRLIKYAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAHEAIS+IF+EEDNKPAPSENLN RIQGFGNSNYE PSEDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHEAISSIFAEEDNKPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RG NLQRSLTTEMEYDNRYEPVEYGRET   LGT+RSTTSGT
Sbjct: 181 LSNFAQGHSSRKNGTSSHRGINLQRSLTTEMEYDNRYEPVEYGRET---LGTARSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NGS SSG SESKTRE+RLLDTIAT+GGVRLQPTRD+IQAF VEA   
Sbjct: 241 WNQDSRVS-----NGSPSSGSSESKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR++DDDHFSIVTSYFSENQ+AVIGCSES
Sbjct: 301 DALALSNALETKLKSPSWQVRFKALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLREKASKV+PLLDGGKGVP MN SEKSLPSNTSSTIQMPDLIDTSDA DY GT  S
Sbjct: 361 PQASLREKASKVMPLLDGGKGVPSMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHT ETRE+ DDL
Sbjct: 421 VEVENL-------SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTIETRENPDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN D+N V+NENKKP  EQKN+PG FDIFGSSSEPA+QEHARKDVNDLMSGLS+HED
Sbjct: 481 FSGMNFDNNQVSNENKKPALEQKNEPGVFDIFGSSSEPAVQEHARKDVNDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
            LKSKDKGDSKDSLS S FS S QPNHQ  + QDSLNG+YSSPM G NMNA F PGM YL
Sbjct: 541 TLKSKDKGDSKDSLSESLFSASGQPNHQNPVSQDSLNGIYSSPMAGTNMNAAFFPGMTYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGG--TAP 660
           P   MF+ AFSSQPM YA +GNFF QQQLLSAMSNYQQFGNPNLQS+ GG V  G  ++P
Sbjct: 601 PSGMMFNPAFSSQPMAYAASGNFFTQQQLLSAMSNYQQFGNPNLQSNSGGGVGSGGYSSP 660

Query: 661 LPDIFHPNLPTQAPSSMMNSSKKEETRAFDFISVSLVSSNSKFS 687
           LPDIF PNL  Q+ +S+MNSSKKE+TRAFDFIS S+     KFS
Sbjct: 661 LPDIFQPNLAAQSSTSVMNSSKKEDTRAFDFISNSVRGYGDKFS 689

BLAST of MS013139 vs. ExPASy TrEMBL
Match: A0A1S3BH26 (VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 PE=4 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 5.3e-306
Identity = 571/693 (82.40%), Postives = 601/693 (86.72%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMID ATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           HKSP+VKQKALRLIKYAVGKSGVEFRREMQR+SVAVRQLFHYKGQ DPLKGDALNKAVRD
Sbjct: 61  HKSPVVKQKALRLIKYAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAHEAISAIFSEEDNKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQG 180
           TAHEAIS+IF+EEDNKPAPSENLN RIQGFGNSNYE PSEDKKSFL EVVGLGSASIKQG
Sbjct: 121 TAHEAISSIFAEEDNKPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNFTQGHSSRKNGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTTSGT 240
           LSNF QGHSSRKNG SS RG NLQRSLTTEMEYDNRYEPVEYGRET   LGT+RSTTSGT
Sbjct: 181 LSNFAQGHSSRKNGTSSHRGINLQRSLTTEMEYDNRYEPVEYGRET---LGTARSTTSGT 240

Query: 241 WNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEAANF 300
           WNQDSRVS     NGS SSG SESKTRE+RLLDTIAT+GGVRLQPTRD+IQAF VEA   
Sbjct: 241 WNQDSRVS-----NGSPSSGSSESKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKL 300

Query: 301 ----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGCSES 360
                            +QVRFKALCILESIVR++DDDHFSIVTSYFSENQ+AVIGCSES
Sbjct: 301 DALALSNALETKLKSPSWQVRFKALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSES 360

Query: 361 PQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGTKNS 420
           PQASLREKASKV+PLLDGGKGVP MN SEKSLPSNTSSTIQMPDLIDTSDA DY GT  S
Sbjct: 361 PQASLREKASKVMPLLDGGKGVPSMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKS 420

Query: 421 QEVENSKNISINPSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREHTDDL 480
            EVEN        SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHT ETRE+ DDL
Sbjct: 421 VEVENL-------SSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTIETRENPDDL 480

Query: 481 FSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLSVHED 540
           FSGMN D+N V+NENKKP  EQKN+PG FDIFGSSSEPA+QEHARKDVNDLMSGLS+HED
Sbjct: 481 FSGMNFDNNQVSNENKKPALEQKNEPGVFDIFGSSSEPAVQEHARKDVNDLMSGLSIHED 540

Query: 541 ALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPGMAYL 600
            LKSKDKGDSKDSLS S FS S QPNHQ  + QDSLNG+YSSPM G NMNA F PGM YL
Sbjct: 541 TLKSKDKGDSKDSLSESLFSASGQPNHQNPVSQDSLNGIYSSPMAGTNMNAAFFPGMTYL 600

Query: 601 PPSTMFSTAFSSQPMGYAPTGNFFAQQQLLSAMSNYQQFGNPNLQSSGGGAVNGG--TAP 660
           P   MF+ AFSSQPM YA +GNFF QQQLLSAMSNYQQFGNPNLQS+ GG V  G  ++P
Sbjct: 601 PSGMMFNPAFSSQPMAYAASGNFFTQQQLLSAMSNYQQFGNPNLQSNSGGGVGSGGYSSP 660

Query: 661 LPDIFHPNLPTQAPSSMMNSSKKEETRAFDFIS 676
           LPDIF PNL  Q+ +S+MNSSKKE+TRAFDFIS
Sbjct: 661 LPDIFQPNLAAQSSTSVMNSSKKEDTRAFDFIS 678

BLAST of MS013139 vs. TAIR 10
Match: AT3G16270.1 (ENTH/VHS family protein )

HSP 1 Score: 639.0 bits (1647), Expect = 4.3e-183
Identity = 388/709 (54.72%), Postives = 484/709 (68.27%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDTATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MD+SRRAVESYWRSRMID  TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL+
Sbjct: 1   MDTSRRAVESYWRSRMIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLD 60

Query: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQLDPLKGDALNKAVRD 120
           +KSPIVKQKALRLIKYAVGKSG EFRREMQR+SVAVR LFHYKG  DPLKGDALNKAVR+
Sbjct: 61  NKSPIVKQKALRLIKYAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRE 120

Query: 121 TAHEAISAIFSEED-NKPAPSENLNSRIQGFGNSNYEVPSEDKKSFLREVVGLGSASIKQ 180
           TAHE ISAIFSEE+  KPA  E++N RI+GFGN+N++VPS D KSFL EVVG+GSASIKQ
Sbjct: 121 TAHETISAIFSEENGTKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQ 180

Query: 181 GLSNFTQGHSSRK--NGASSQRGPNLQRSLTTEMEYDNRYEPVEYGRETHGSLGTSRSTT 240
           G+SNF QGH  +K  NG+SS RGPNL RSLT E E  +RY+PV+ G++  G+ GTS++TT
Sbjct: 181 GISNFAQGHLPKKNENGSSSYRGPNLHRSLTMENENFSRYDPVKLGKD--GNYGTSKNTT 240

Query: 241 SGTWNQDSRVSKVEPTNGSLSSGFSESKTREERLLDTIATSGGVRLQPTRDAIQAFFVEA 300
            G+W   S        + S +S   ESKTREE+LL+TI TSGGVRLQPTRDA+  F +EA
Sbjct: 241 GGSWGHAS-----GEASESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEA 300

Query: 301 ANF----------------IFQVRFKALCILESIVRKDDDDHFSIVTSYFSENQDAVIGC 360
           A                  ++QVR KALC+LE+I+RK +D++FSIV +YFSEN DA+  C
Sbjct: 301 AKMDAVALSIALDGKLHSPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRC 360

Query: 361 SESPQASLREKASKVIPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLIDTSDADDYGGT 420
           +ESPQ+SLREKA+KV+ LL+GG+    M+ S+ ++     + + +PDLIDT D+DD    
Sbjct: 361 AESPQSSLREKANKVLSLLNGGQSSGLMSSSDNTV--KREAAVDLPDLIDTGDSDD--TL 420

Query: 421 KNSQEVENSKNISINPSSTPLV-DDLFGDGLNTVTSTSELKNDDDPFSDVSFHTTETREH 480
            N   ++    ++   ++ PL+ DD FGD  +   S+SE K DDDPF+DVSFH  E +E 
Sbjct: 421 NNLNAIDTGSTVA---TAGPLMDDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKES 480

Query: 481 TDDLFSGMNVDSNHVTNENKKPVSEQKNDPGGFDIFGSSSEPALQEHARKDVNDLMSGLS 540
            DDLFSGM V         K       + P  FD+FGS+++   +    K++NDLM   S
Sbjct: 481 ADDLFSGMTVG-------EKSAAVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFS 540

Query: 541 VHEDALKSKDKGDSKDSLSASSFSVSSQPNHQYQIPQDSLNGMYSSPMVGANMNATFLPG 600
           + E+   S  KG S  +L    F++ S  +H  Q P++ + G+  S   G   N T LPG
Sbjct: 541 IDEN--NSNQKGSSSSTLPQDLFAMPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPG 600

Query: 601 --MAY-LPPSTMFSTAFSSQPMGYAPTGNFFA-QQQLLSAMSNYQQFGNPNLQSSGG--- 660
             M +  P   M + AF+SQP+ YA   +  A QQQ L  MSN+QQFGN N Q SG    
Sbjct: 601 GVMPFNFPQGMMMNPAFASQPLNYAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLS 660

Query: 661 -GAVNGGTAPLPDIFHPNLPTQAPSSMMNSSKKEETRAFDFISVSLVSS 682
            G   G  + LPDIF PN   QAP+S MN SKKE+TRAFDFIS  L S+
Sbjct: 661 MGTSGGNQSALPDIFQPNFGNQAPTSTMNGSKKEDTRAFDFISDHLTSA 683