MS013081 (gene) Bitter gourd (TR) v1

Overview
NameMS013081
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReceptor-like serine/threonine-protein kinase
Locationscaffold459: 74883 .. 77523 (+)
RNA-Seq ExpressionMS013081
SyntenyMS013081
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTCCACAAAATCTCAAGTTTCTTCTTCTCCTTCTTCCTGATTTCTCAATCCCTCATCGTCGAATCCCAAAATATCGACAAGATCCTCCCAGGCTTCAAAGCTTCTGCATCAGAATTCAACCAAACAAACGGGAATTTTCTTCTGTCCAACAACTCTGTTTTCGCTCTCGGCTTCTACAGCGGCGCCGATGACAACGTTTTCTCACTCGGAATCACTCACATTTTCAGTTCAAAGGTAATCTGGACGGCCAACAGAGATTTTCCGGTGAACGATTCTGCACTCTTTGTATTTGATGAAACAGGGGTTGCTTATTTGGATGGTTCCGGCCGGAATCGGGCTCCGGTTTGGTCGACGGGGACGGCCGGAGACGGCGTCGTTTCGATGCAGCTGTTGGATTCCGGGAACTTGGTTTTGCAGAGTAACAATGGAAGTTTTATTTGGCAGAGCTTCCATTTTCCGACCGATACCCTTTTGCCCGGCCAGGTTTTTTGGGAAGGAATGAGGCTCAGAAGTTATCCAAACGACAATGATCTCTCGAATTTTTTGGAATTTAAACATGGCGATTTGGTTCTCTCCGCCGGTTACCGGAATCCCCAAATCTATTGGGCTCTGTCGAATGATAGCCGGAAAATTCCAAAGTCCGCCGCCGGTTCCGGTGGAGGTGGGTATGTTCTGTTTGCTATTTTGGAGTCGAATTCTTGGAATTTCTACGGCCAGAAAGGGGAATTGTTATGGGAATTCAGATTTTTCTGGCAATCGAGTTGGAAAGACAGATGGGTCTCTGTTTTAAACACCGACGGATCGATTTCCTTTCACAATTTGGAGATGGGAAAATCAGCTCCGCCGGAGCCGATTCGAATTCCGGCGGGAAATTGCGGCGTTCCGGAGCCTTGTGATCCTCTTTTCATCTGCTACTTCGACAACCGCTGCCAATGCCCTTCGATTCTCAACGAGAAAATCAACTGCAAAATCCCTTCGATCCCCTGTAATGGCAGCTCCACCGAGCTTCTGTATTTGGGGAAAGATCTTGATTACTTCGCTCTTCGCTTCTCAACTCCTTCATTCAACTCCGACTTGAACTCCTGCAAAGCGGCCTGCGCCGGTAATTGCTCTTGCCACGTTTTATTCTTCGAACCCATTTCGGGAAATTGCTTCCTCTTCGACCAAATCGGTAGTTTACAGCGGTCGGAGGAAAGCTCCGGCGGGTACATTTCGTATATGAAAACGAAGCTCCCCATTAATGGCGGCGACGCCGAGATGAGCCCAAGCCAGTACAGAAGAAGAAAACACATCGTGCTGATGAGCATTCTTCTCGCCGCAATGGCTCTGGCATTTATGGGCCTGCTCTGTTTTCGGTTCTACCGCCGGAAAATGAAGGAACTGCTAAGCTCCATCGAAGACGTAACAGAGGAAGACAAATTCCTCGAGCAGATCTCCGGTGGGCCGATGCGGTTCAGTTTCCGGCAGCTCCGGCGAGCAACGAAGAATTTCTCGAGGAAAATCGGGCACGGGGGATTCGGGTCGGTTTATTTGGGGGAGGTTGGAGATGGGTCGAGAGTGGCGGTGAAGAAACTGGAGCGAATGGGGCAAGGGGCGAGGGAATTCAGAGCGGAGGTCAGTTTGATCGGCGGGATCCACCATGTGAATCTGGTGAAGCTCACAGGATTCTGCTCTGAAAGTCTCCATCGGCTTCTGGTGTACGAGTATATGAGCAACGGATCGTTGGATAAATGGATTTTCAATGGTGAAGAAGACGCTCCGTTTCTGGATTGGGACACCCGATTCAACATCGCATTGGGTAAACTCTTAACCAAATTCCTCACTTTCAATTTTGTGTTCTACTCCTAACTTGAACTTAGTAAGCTTAGGAAGTGGAATAGAGGGTGGTAAAAATAAAACTGCAGTCCTAGTTTCTGTAATTAAAGCTGGGGGAATCAATATTTTGATATGAACAAAATTGTTTGGGCCATTTCATCGAACAGGTATAGCAAGGGCAGTGGCGTACCTCCACGAAGAATGCGAATCAAAGATAATCCACTGCGACATAAAGCCAGAGAACGTCCTTCTGGACCAAAATTTCACACCCAAAGTCTCCGATTTCGGGATGGCCAAGTTAATGGACGCACAAAAGAGCTTCATCTGCACCCAGCTTCGCGGAACCAGAGGCTACGTCGCGCCGGAGTGGATCACCCACCTCGCAATCTCCGACAAGACCGACGTCTACAGCTACGGCGTCCTGCTTCTCGAGATCATCGCCGGGAGGAAGAGCTACCACGCCGACTCCGAACAGCCGCCGGAGAAGGCGCATCTGCCGTCCTACGCGATGAAGATGGTGGCGGAGCAGAAGGGGCGTCGGGTTCTGGACCCGCGGCTGCAGGTGGAGGAGGCCGACCGGCGGGTGGAGGCGGCGGTGGAAGTGGCGGTGTGGTGCATTCAGGAGGAGGTCAGTCTCCGGCCGCCGATGAGGAAGGTGGTGCAGATGCTGGAGGGGGTTTCTCCGGTGCCCAAGCCGCCGATCTGGTCGGAGATGGGTTCGAGTTTTTCGTGGAGCAGTGGAGCGGGGGCAATGTTATTGGGATCCAATGGGTATTTTAGTGAAGTTCGGTTGTCGGATGTTCAATTATCCGGGCCGAGA

mRNA sequence

ATGGGTTTCCACAAAATCTCAAGTTTCTTCTTCTCCTTCTTCCTGATTTCTCAATCCCTCATCGTCGAATCCCAAAATATCGACAAGATCCTCCCAGGCTTCAAAGCTTCTGCATCAGAATTCAACCAAACAAACGGGAATTTTCTTCTGTCCAACAACTCTGTTTTCGCTCTCGGCTTCTACAGCGGCGCCGATGACAACGTTTTCTCACTCGGAATCACTCACATTTTCAGTTCAAAGGTAATCTGGACGGCCAACAGAGATTTTCCGGTGAACGATTCTGCACTCTTTGTATTTGATGAAACAGGGGTTGCTTATTTGGATGGTTCCGGCCGGAATCGGGCTCCGGTTTGGTCGACGGGGACGGCCGGAGACGGCGTCGTTTCGATGCAGCTGTTGGATTCCGGGAACTTGGTTTTGCAGAGTAACAATGGAAGTTTTATTTGGCAGAGCTTCCATTTTCCGACCGATACCCTTTTGCCCGGCCAGGTTTTTTGGGAAGGAATGAGGCTCAGAAGTTATCCAAACGACAATGATCTCTCGAATTTTTTGGAATTTAAACATGGCGATTTGGTTCTCTCCGCCGGTTACCGGAATCCCCAAATCTATTGGGCTCTGTCGAATGATAGCCGGAAAATTCCAAAGTCCGCCGCCGGTTCCGGTGGAGGTGGGTATGTTCTGTTTGCTATTTTGGAGTCGAATTCTTGGAATTTCTACGGCCAGAAAGGGGAATTGTTATGGGAATTCAGATTTTTCTGGCAATCGAGTTGGAAAGACAGATGGGTCTCTGTTTTAAACACCGACGGATCGATTTCCTTTCACAATTTGGAGATGGGAAAATCAGCTCCGCCGGAGCCGATTCGAATTCCGGCGGGAAATTGCGGCGTTCCGGAGCCTTGTGATCCTCTTTTCATCTGCTACTTCGACAACCGCTGCCAATGCCCTTCGATTCTCAACGAGAAAATCAACTGCAAAATCCCTTCGATCCCCTGTAATGGCAGCTCCACCGAGCTTCTGTATTTGGGGAAAGATCTTGATTACTTCGCTCTTCGCTTCTCAACTCCTTCATTCAACTCCGACTTGAACTCCTGCAAAGCGGCCTGCGCCGGTAATTGCTCTTGCCACGTTTTATTCTTCGAACCCATTTCGGGAAATTGCTTCCTCTTCGACCAAATCGGTAGTTTACAGCGGTCGGAGGAAAGCTCCGGCGGGTACATTTCGTATATGAAAACGAAGCTCCCCATTAATGGCGGCGACGCCGAGATGAGCCCAAGCCAGTACAGAAGAAGAAAACACATCGTGCTGATGAGCATTCTTCTCGCCGCAATGGCTCTGGCATTTATGGGCCTGCTCTGTTTTCGGTTCTACCGCCGGAAAATGAAGGAACTGCTAAGCTCCATCGAAGACGTAACAGAGGAAGACAAATTCCTCGAGCAGATCTCCGGTGGGCCGATGCGGTTCAGTTTCCGGCAGCTCCGGCGAGCAACGAAGAATTTCTCGAGGAAAATCGGGCACGGGGGATTCGGGTCGGTTTATTTGGGGGAGGTTGGAGATGGGTCGAGAGTGGCGGTGAAGAAACTGGAGCGAATGGGGCAAGGGGCGAGGGAATTCAGAGCGGAGGTCAGTTTGATCGGCGGGATCCACCATGTGAATCTGGTGAAGCTCACAGGATTCTGCTCTGAAAGTCTCCATCGGCTTCTGGTGTACGAGTATATGAGCAACGGATCGTTGGATAAATGGATTTTCAATGGTGAAGAAGACGCTCCGTTTCTGGATTGGGACACCCGATTCAACATCGCATTGGGTATAGCAAGGGCAGTGGCGTACCTCCACGAAGAATGCGAATCAAAGATAATCCACTGCGACATAAAGCCAGAGAACGTCCTTCTGGACCAAAATTTCACACCCAAAGTCTCCGATTTCGGGATGGCCAAGTTAATGGACGCACAAAAGAGCTTCATCTGCACCCAGCTTCGCGGAACCAGAGGCTACGTCGCGCCGGAGTGGATCACCCACCTCGCAATCTCCGACAAGACCGACGTCTACAGCTACGGCGTCCTGCTTCTCGAGATCATCGCCGGGAGGAAGAGCTACCACGCCGACTCCGAACAGCCGCCGGAGAAGGCGCATCTGCCGTCCTACGCGATGAAGATGGTGGCGGAGCAGAAGGGGCGTCGGGTTCTGGACCCGCGGCTGCAGGTGGAGGAGGCCGACCGGCGGGTGGAGGCGGCGGTGGAAGTGGCGGTGTGGTGCATTCAGGAGGAGGTCAGTCTCCGGCCGCCGATGAGGAAGGTGGTGCAGATGCTGGAGGGGGTTTCTCCGGTGCCCAAGCCGCCGATCTGGTCGGAGATGGGTTCGAGTTTTTCGTGGAGCAGTGGAGCGGGGGCAATGTTATTGGGATCCAATGGGTATTTTAGTGAAGTTCGGTTGTCGGATGTTCAATTATCCGGGCCGAGA

Coding sequence (CDS)

ATGGGTTTCCACAAAATCTCAAGTTTCTTCTTCTCCTTCTTCCTGATTTCTCAATCCCTCATCGTCGAATCCCAAAATATCGACAAGATCCTCCCAGGCTTCAAAGCTTCTGCATCAGAATTCAACCAAACAAACGGGAATTTTCTTCTGTCCAACAACTCTGTTTTCGCTCTCGGCTTCTACAGCGGCGCCGATGACAACGTTTTCTCACTCGGAATCACTCACATTTTCAGTTCAAAGGTAATCTGGACGGCCAACAGAGATTTTCCGGTGAACGATTCTGCACTCTTTGTATTTGATGAAACAGGGGTTGCTTATTTGGATGGTTCCGGCCGGAATCGGGCTCCGGTTTGGTCGACGGGGACGGCCGGAGACGGCGTCGTTTCGATGCAGCTGTTGGATTCCGGGAACTTGGTTTTGCAGAGTAACAATGGAAGTTTTATTTGGCAGAGCTTCCATTTTCCGACCGATACCCTTTTGCCCGGCCAGGTTTTTTGGGAAGGAATGAGGCTCAGAAGTTATCCAAACGACAATGATCTCTCGAATTTTTTGGAATTTAAACATGGCGATTTGGTTCTCTCCGCCGGTTACCGGAATCCCCAAATCTATTGGGCTCTGTCGAATGATAGCCGGAAAATTCCAAAGTCCGCCGCCGGTTCCGGTGGAGGTGGGTATGTTCTGTTTGCTATTTTGGAGTCGAATTCTTGGAATTTCTACGGCCAGAAAGGGGAATTGTTATGGGAATTCAGATTTTTCTGGCAATCGAGTTGGAAAGACAGATGGGTCTCTGTTTTAAACACCGACGGATCGATTTCCTTTCACAATTTGGAGATGGGAAAATCAGCTCCGCCGGAGCCGATTCGAATTCCGGCGGGAAATTGCGGCGTTCCGGAGCCTTGTGATCCTCTTTTCATCTGCTACTTCGACAACCGCTGCCAATGCCCTTCGATTCTCAACGAGAAAATCAACTGCAAAATCCCTTCGATCCCCTGTAATGGCAGCTCCACCGAGCTTCTGTATTTGGGGAAAGATCTTGATTACTTCGCTCTTCGCTTCTCAACTCCTTCATTCAACTCCGACTTGAACTCCTGCAAAGCGGCCTGCGCCGGTAATTGCTCTTGCCACGTTTTATTCTTCGAACCCATTTCGGGAAATTGCTTCCTCTTCGACCAAATCGGTAGTTTACAGCGGTCGGAGGAAAGCTCCGGCGGGTACATTTCGTATATGAAAACGAAGCTCCCCATTAATGGCGGCGACGCCGAGATGAGCCCAAGCCAGTACAGAAGAAGAAAACACATCGTGCTGATGAGCATTCTTCTCGCCGCAATGGCTCTGGCATTTATGGGCCTGCTCTGTTTTCGGTTCTACCGCCGGAAAATGAAGGAACTGCTAAGCTCCATCGAAGACGTAACAGAGGAAGACAAATTCCTCGAGCAGATCTCCGGTGGGCCGATGCGGTTCAGTTTCCGGCAGCTCCGGCGAGCAACGAAGAATTTCTCGAGGAAAATCGGGCACGGGGGATTCGGGTCGGTTTATTTGGGGGAGGTTGGAGATGGGTCGAGAGTGGCGGTGAAGAAACTGGAGCGAATGGGGCAAGGGGCGAGGGAATTCAGAGCGGAGGTCAGTTTGATCGGCGGGATCCACCATGTGAATCTGGTGAAGCTCACAGGATTCTGCTCTGAAAGTCTCCATCGGCTTCTGGTGTACGAGTATATGAGCAACGGATCGTTGGATAAATGGATTTTCAATGGTGAAGAAGACGCTCCGTTTCTGGATTGGGACACCCGATTCAACATCGCATTGGGTATAGCAAGGGCAGTGGCGTACCTCCACGAAGAATGCGAATCAAAGATAATCCACTGCGACATAAAGCCAGAGAACGTCCTTCTGGACCAAAATTTCACACCCAAAGTCTCCGATTTCGGGATGGCCAAGTTAATGGACGCACAAAAGAGCTTCATCTGCACCCAGCTTCGCGGAACCAGAGGCTACGTCGCGCCGGAGTGGATCACCCACCTCGCAATCTCCGACAAGACCGACGTCTACAGCTACGGCGTCCTGCTTCTCGAGATCATCGCCGGGAGGAAGAGCTACCACGCCGACTCCGAACAGCCGCCGGAGAAGGCGCATCTGCCGTCCTACGCGATGAAGATGGTGGCGGAGCAGAAGGGGCGTCGGGTTCTGGACCCGCGGCTGCAGGTGGAGGAGGCCGACCGGCGGGTGGAGGCGGCGGTGGAAGTGGCGGTGTGGTGCATTCAGGAGGAGGTCAGTCTCCGGCCGCCGATGAGGAAGGTGGTGCAGATGCTGGAGGGGGTTTCTCCGGTGCCCAAGCCGCCGATCTGGTCGGAGATGGGTTCGAGTTTTTCGTGGAGCAGTGGAGCGGGGGCAATGTTATTGGGATCCAATGGGTATTTTAGTGAAGTTCGGTTGTCGGATGTTCAATTATCCGGGCCGAGA

Protein sequence

MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNRCQCPSILNEKINCKIPSIPCNGSSTELLYLGKDLDYFALRFSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDAEMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRLQVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWSEMGSSFSWSSGAGAMLLGSNGYFSEVRLSDVQLSGPR
Homology
BLAST of MS013081 vs. NCBI nr
Match: XP_022153573.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Momordica charantia])

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 660/667 (98.95%), Postives = 662/667 (99.25%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60
           MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF
Sbjct: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60

Query: 61  YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST 120
           YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST
Sbjct: 61  YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST 120

Query: 121 GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL 180
           GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL
Sbjct: 121 GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL 180

Query: 181 SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG 240
           SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG
Sbjct: 181 SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG 240

Query: 241 QKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC 300
           QKGELLWEFRFFWQS+WKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC
Sbjct: 241 QKGELLWEFRFFWQSNWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC 300

Query: 301 DPLFICYFDNRCQCPSILNEKINCKIPSIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD 360
           DPLFICYFDNRCQCPSILNEKINCKI SIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD
Sbjct: 301 DPLFICYFDNRCQCPSILNEKINCKIHSIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD 360

Query: 361 LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA 420
           LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA
Sbjct: 361 LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA 420

Query: 421 EMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQI 480
           EMSPSQYRRRKHIVLMSILLAAMALAFMGL CFRFYRRKMKELLSSIE+ TEEDKFLEQI
Sbjct: 421 EMSPSQYRRRKHIVLMSILLAAMALAFMGLFCFRFYRRKMKELLSSIEEATEEDKFLEQI 480

Query: 481 SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE 540
           SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE
Sbjct: 481 SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE 540

Query: 541 VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA 600
           VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA
Sbjct: 541 VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA 600

Query: 601 LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG 660
           LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG
Sbjct: 601 LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG 660

Query: 661 TRGYVAP 668
           TRG V P
Sbjct: 661 TRGLVDP 667

BLAST of MS013081 vs. NCBI nr
Match: XP_022998179.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita maxima])

HSP 1 Score: 1308.9 bits (3386), Expect = 0.0e+00
Identity = 650/832 (78.12%), Postives = 728/832 (87.50%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSL--IVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFAL 60
           MG  KI+SFFF F  + QS   IV+SQ IDK+ PGFKASASEFN TNG FLLS  S+F+L
Sbjct: 1   MGLFKIASFFFFFVFLIQSHIDIVKSQEIDKLNPGFKASASEFNDTNGVFLLSKGSIFSL 60

Query: 61  GFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 120
           GFY+GA+D+ FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG AYLD  G N AP+W
Sbjct: 61  GFYAGANDDTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDAYLDQFGPNSAPIW 120

Query: 121 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 180
           ST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY NDN
Sbjct: 121 STETARHGVVSMQLLDSGNLVLKSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLVSYSNDN 180

Query: 181 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILESN 240
           +LS+FLEFK GDLVLSAGY+NPQIYWALS DSRKI ++AAGSG G    GYVLFAI+ESN
Sbjct: 181 NLSSFLEFKQGDLVLSAGYQNPQIYWALSKDSRKIKRAAAGSGSGNGGDGYVLFAIMESN 240

Query: 241 SWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNC 300
           SWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +C
Sbjct: 241 SWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAESC 300

Query: 301 GVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNGSS--TELLYLGKDLDYFALR 360
           GVPEPCDPLFICYFDNRCQCPS I ++K NCK PSI CNG S  TELLYLGK+LDYFALR
Sbjct: 301 GVPEPCDPLFICYFDNRCQCPSTIFDDKFNCKFPSISCNGRSNATELLYLGKNLDYFALR 360

Query: 361 FSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKT 420
           F+ P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK 
Sbjct: 361 FAIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMKI 420

Query: 421 KLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDV 480
           +LPIN  D E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED 
Sbjct: 421 ELPINSNDNETTPTPTPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIEDA 480

Query: 481 TEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERM 540
           TEEDKFLE++S GPMRFS+RQLRRAT+NFS KIG GGFGSVYLGE+GDG+R+AVKKLE++
Sbjct: 481 TEEDKFLEEVSSGPMRFSYRQLRRATRNFSTKIGKGGFGSVYLGELGDGTRLAVKKLEKI 540

Query: 541 GQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPF 600
           GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  F
Sbjct: 541 GQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG-F 600

Query: 601 LDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQ 660
           LDW+TRFNIALG ARA+AYLH+ECESKIIHCDIKPENVLLD NFTPK+SDFGMAKL++ +
Sbjct: 601 LDWETRFNIALGTARALAYLHQECESKIIHCDIKPENVLLDDNFTPKLSDFGMAKLVNRE 660

Query: 661 KSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAH 720
           +S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG++LLEII  RK Y  D++QPPE AH
Sbjct: 661 QSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYGMVLLEIIGNRKCY--DADQPPESAH 720

Query: 721 LPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQM 780
           LPSYA +MVAE+KGR VLDPR+   V E D RVEAAVEVAVWC+QEE S RPPMRKVVQM
Sbjct: 721 LPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEAAVEVAVWCVQEEASFRPPMRKVVQM 780

Query: 781 LEGVSPVPKPPIWSEMGSSFSWSSGAG-AMLLGSNGYFSEVRLSDVQLSGPR 820
           LEGV PVP+PP  +E+G SFSWSSG G  M LG NG FSEVRLSDV+LSGPR
Sbjct: 781 LEGVCPVPRPPSAAEIGWSFSWSSGGGTVMSLGLNGCFSEVRLSDVRLSGPR 829

BLAST of MS013081 vs. NCBI nr
Match: XP_023523258.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1307.7 bits (3383), Expect = 0.0e+00
Identity = 649/833 (77.91%), Postives = 726/833 (87.15%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSL--IVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFAL 60
           MG  KI+SFFF F  + QS   IV+SQ IDK+ PGFKASASE N TNG FLLS  S+FAL
Sbjct: 1   MGLFKIASFFFFFVFLIQSHIDIVKSQEIDKLNPGFKASASELNHTNGVFLLSKASIFAL 60

Query: 61  GFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 120
           GFY+GA+D  FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG AYLD  G N AP+W
Sbjct: 61  GFYTGANDGTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDAYLDQFGPNSAPIW 120

Query: 121 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 180
           ST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY NDN
Sbjct: 121 STETARHGVVSMQLLDSGNLVLRSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLESYSNDN 180

Query: 181 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILESN 240
           +LS+FLEFK GDLVLSAGY+NPQ+YWALSND RKI ++AAGSG G    GYVLFAI+ESN
Sbjct: 181 NLSSFLEFKQGDLVLSAGYQNPQVYWALSNDGRKIKRAAAGSGSGNGGDGYVLFAIMESN 240

Query: 241 SWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNC 300
           SWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +C
Sbjct: 241 SWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAESC 300

Query: 301 GVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNGSS--TELLYLGKDLDYFALR 360
           GVPEPCDPLFICYFDNRCQC S IL++K NCK PSI CNG S  TELLYLGK+LDYFALR
Sbjct: 301 GVPEPCDPLFICYFDNRCQCASTILDDKFNCKFPSISCNGRSNATELLYLGKNLDYFALR 360

Query: 361 FSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKT 420
           FS P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK 
Sbjct: 361 FSIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMKI 420

Query: 421 KLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDV 480
           +LPIN  + E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED 
Sbjct: 421 ELPINSNNNETTPTPTPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIEDA 480

Query: 481 TEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERM 540
           TEEDKFLE++S GPMRFS+RQLRRAT+NFS KIGHGGFGSVYLGE+GDG+R+AVKKLE++
Sbjct: 481 TEEDKFLEEVSSGPMRFSYRQLRRATRNFSTKIGHGGFGSVYLGELGDGTRLAVKKLEKI 540

Query: 541 GQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPF 600
           GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  F
Sbjct: 541 GQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG-F 600

Query: 601 LDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQ 660
           LDW+TRFNIALG ARA+AYLH+ECESKIIHCDIKPENVLLD NFTPK+SDFGMAKL++ +
Sbjct: 601 LDWETRFNIALGTARALAYLHQECESKIIHCDIKPENVLLDDNFTPKLSDFGMAKLVNRE 660

Query: 661 KSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAH 720
           +S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG++LLEI+A RK Y  D++Q PE AH
Sbjct: 661 QSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYGMVLLEIVANRKCY--DADQSPESAH 720

Query: 721 LPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQM 780
           LPSYA +MVAE+KGR VLDPR+   V E D RVEA VEVAVWC+QEE S RPPMRKVVQM
Sbjct: 721 LPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEAVVEVAVWCVQEEASFRPPMRKVVQM 780

Query: 781 LEGVSPVPKPPIWSEMGSSFSWSSGAG--AMLLGSNGYFSEVRLSDVQLSGPR 820
           LEGV PVP+PP  +EMG SFSWSSG G   M LG NG FSEVRLSDV+LSGPR
Sbjct: 781 LEGVCPVPRPPSAAEMGRSFSWSSGGGGTVMSLGLNGCFSEVRLSDVRLSGPR 830

BLAST of MS013081 vs. NCBI nr
Match: XP_022948682.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita moschata])

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 647/833 (77.67%), Postives = 726/833 (87.15%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSL--IVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFAL 60
           MG  KI+SFFF F  + QS   IV+ Q IDK+ PGFKASASE N TNG FLLS  S+FAL
Sbjct: 1   MGLFKIASFFFFFVFLIQSHIDIVKCQEIDKLNPGFKASASELNHTNGVFLLSKASIFAL 60

Query: 61  GFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 120
           GFY+GA+D  FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG  YLD  G N AP+W
Sbjct: 61  GFYAGANDGTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDTYLDQFGPNSAPIW 120

Query: 121 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 180
           ST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY NDN
Sbjct: 121 STETARHGVVSMQLLDSGNLVLRSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLESYSNDN 180

Query: 181 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILESN 240
           +LS+FLEFK GDLVLSAGY+NPQ+YWALSND RKI ++AAGSG G    GYVLFAI+ESN
Sbjct: 181 NLSSFLEFKQGDLVLSAGYQNPQVYWALSNDGRKIKRAAAGSGSGNGGDGYVLFAIMESN 240

Query: 241 SWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNC 300
           SWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +C
Sbjct: 241 SWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAESC 300

Query: 301 GVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNG--SSTELLYLGKDLDYFALR 360
           GVPEPCDPLFICYFDNRCQC S IL++K NCK PSI CNG  +STELLYLGK+LDYFALR
Sbjct: 301 GVPEPCDPLFICYFDNRCQCASTILDDKFNCKFPSISCNGRSNSTELLYLGKNLDYFALR 360

Query: 361 FSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKT 420
           FS P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK 
Sbjct: 361 FSIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMKI 420

Query: 421 KLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDV 480
           +LPIN  + E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED 
Sbjct: 421 ELPINSNNNETTPTPTPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIEDA 480

Query: 481 TEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERM 540
           TEEDKFLE++S GPMRFS+RQLRRAT+NFS KIGHGGFGSVYLG++GDG+R+AVKKLE++
Sbjct: 481 TEEDKFLEEVSSGPMRFSYRQLRRATRNFSTKIGHGGFGSVYLGDLGDGTRLAVKKLEKI 540

Query: 541 GQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPF 600
           GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  F
Sbjct: 541 GQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG-F 600

Query: 601 LDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQ 660
           LDW+TRFNIALG ARA+AYLH+ECESKIIHCDIKPENVLLD NFTPK+SDFGMAKL++ +
Sbjct: 601 LDWETRFNIALGTARALAYLHQECESKIIHCDIKPENVLLDDNFTPKLSDFGMAKLVNRE 660

Query: 661 KSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAH 720
           +S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG++LLEI+A RK Y  D++Q PE AH
Sbjct: 661 QSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYGMVLLEIVANRKCY--DADQSPESAH 720

Query: 721 LPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQM 780
           LPSYA +MVAE+KGR VLDPR+   V E D RVEA VEVAVWC+QEE SLRPPMRKVVQM
Sbjct: 721 LPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEAVVEVAVWCVQEEASLRPPMRKVVQM 780

Query: 781 LEGVSPVPKPPIWSEMGSSFSWSSGAG--AMLLGSNGYFSEVRLSDVQLSGPR 820
           LEGV PVP+PP  +EMG SFSWSSG G   M LG NG FSEVRLSDV+LSGPR
Sbjct: 781 LEGVCPVPRPPSAAEMGRSFSWSSGGGGTVMSLGLNGCFSEVRLSDVRLSGPR 830

BLAST of MS013081 vs. NCBI nr
Match: KAG6606798.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 650/858 (75.76%), Postives = 727/858 (84.73%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLI---SQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFA 60
           MG  KI+SFFF FF+    S   IV+SQ IDK+ PGFKASASE N TNG FLLS  S+FA
Sbjct: 1   MGLFKIASFFFFFFVFLNRSHIDIVKSQEIDKLNPGFKASASELNHTNGVFLLSKASIFA 60

Query: 61  LGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPV 120
           LGFY+GA+D  FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG AYLD  G N AP+
Sbjct: 61  LGFYAGANDGTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDAYLDQFGPNLAPI 120

Query: 121 WSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPND 180
           WST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY ND
Sbjct: 121 WSTETARHGVVSMQLLDSGNLVLRSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLESYSND 180

Query: 181 NDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILES 240
           N+LS+FLEFK GDLVLSAGY+NPQ+YWALSND RKI ++AAGSG G    GYVLFAI+ES
Sbjct: 181 NNLSSFLEFKQGDLVLSAGYQNPQVYWALSNDGRKIKRAAAGSGSGNGGDGYVLFAIMES 240

Query: 241 NSWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGN 300
           NSWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +
Sbjct: 241 NSWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAES 300

Query: 301 CGVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNG--SSTELLYLGKDLDYFAL 360
           CGVPEPCDPLFICYFDNRCQC S IL++K NCK PSI CNG  +STELLYLGK+L+YFAL
Sbjct: 301 CGVPEPCDPLFICYFDNRCQCASTILDDKFNCKFPSISCNGGSNSTELLYLGKNLEYFAL 360

Query: 361 RFSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMK 420
           RFS P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK
Sbjct: 361 RFSIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMK 420

Query: 421 TKLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIED 480
            +LPIN  D E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED
Sbjct: 421 IELPINSNDNETTPTPIPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIED 480

Query: 481 VTEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLER 540
            TEEDKFLE++S GPMRFS RQLRRAT+NFS KIGHGGFGSVYLGE+GDG+R+AVKKLE+
Sbjct: 481 ATEEDKFLEEVSSGPMRFSHRQLRRATRNFSTKIGHGGFGSVYLGELGDGTRLAVKKLEK 540

Query: 541 MGQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAP 600
           +GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  
Sbjct: 541 IGQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG- 600

Query: 601 FLDWDTRFNIAL------------------------GIARAVAYLHEECESKIIHCDIKP 660
           FLDW+TRFNIAL                        G ARA+AYLH+ECESKIIHCDIKP
Sbjct: 601 FLDWETRFNIALGNISNSRLYECSKLYECSNLVDQTGTARALAYLHQECESKIIHCDIKP 660

Query: 661 ENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYG 720
           ENVLLD NFTPK+SDFGMAKL++ ++S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG
Sbjct: 661 ENVLLDDNFTPKLSDFGMAKLVNREQSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYG 720

Query: 721 VLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEA 780
           ++LLEI+A RK Y  D++Q PE AHLPSYA +MVAE+KGR VLDPR+   V E D RVEA
Sbjct: 721 MVLLEIVANRKCY--DADQSPESAHLPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEA 780

Query: 781 AVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWSEMGSSFSWSSGAG--AMLLGS 820
            VEVAVWC+QEE SLRPPMRKVVQMLEGV PVP+PP  +EMG SFSWSSG G   M LG 
Sbjct: 781 LVEVAVWCVQEEASLRPPMRKVVQMLEGVCPVPRPPSAAEMGRSFSWSSGGGGTVMSLGL 840

BLAST of MS013081 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 2.4e-201
Identity = 377/812 (46.43%), Postives = 520/812 (64.04%), Query Frame = 0

Query: 26  NIDKILPGFKASASEFNQTNGNFLLSNNSVFALGFYSGADD-NVFSLGITHIFSSKVIWT 85
           +I  I PGF  S   +   +G FL SNNS F  GF +  D   +F+L I H  S+K+IW+
Sbjct: 25  SIGSITPGFGGSQMNYINNDGIFLESNNSAFGFGFVTTQDSVTLFTLSIIHKSSTKLIWS 84

Query: 86  ANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNN 145
           ANR  PV++S  FVFD+ G   ++G+      VW    +G     ++L DSGNLV+ S +
Sbjct: 85  ANRASPVSNSDKFVFDDNGNVVMEGT-----EVWRLDNSGKNASRIELRDSGNLVVVSVD 144

Query: 146 GSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYW 205
           G+ IW+SF  PTDTL+  Q F EGM+L S P+ ++++  LE K GD+VLS     PQ+YW
Sbjct: 145 GTSIWESFDHPTDTLITNQAFKEGMKLTSSPSSSNMTYALEIKSGDMVLSVNSLTPQVYW 204

Query: 206 ALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYGQKGELLWEFRFFWQSSWKDRWVSV 265
           +++N   +I         GG V  + L  NSW F+ QK  LLW+F F         W++V
Sbjct: 205 SMANARERIINK-----DGGVVTSSSLLGNSWRFFDQKQVLLWQFVFSDNKDDNTTWIAV 264

Query: 266 LNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNRCQCPSILNE-KIN 325
           L  +G ISF NL  G SA     +IP+  CG PEPC P ++C     C C S L+  + +
Sbjct: 265 LGNNGVISFSNLGSGASAADSSTKIPSDLCGTPEPCGPYYVCSGSKVCGCVSGLSRARSD 324

Query: 326 CKIP-SIPCNGSS------TELLYLGKDLDYFALRFSTP-SFNSDLNSCKAACAGNCSCH 385
           CK   + PC  +        +L+  G  +DYFAL ++ P S  +DL+SCK  C  NCSC 
Sbjct: 325 CKTGITSPCKKTKDNATLPLQLVSAGDGVDYFALGYAPPFSKKTDLDSCKEFCHNNCSCL 384

Query: 386 VLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDAEMSPSQYRRRKHIVL 445
            LFF+  SGNCFLFD IGS + S     G++SY+K     +GG  +      +   ++V+
Sbjct: 385 GLFFQNSSGNCFLFDYIGSFKTSGNGGSGFVSYIKIASTGSGG-GDNGEDDGKHFPYVVI 444

Query: 446 MSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQLRRA 505
           + +++    +A +  + FR ++RK K +L + ++ +EED FLE +SG P+RF+++ L+ A
Sbjct: 445 I-VVVTVFIIAVLIFVAFRIHKRK-KMILEAPQESSEEDNFLENLSGMPIRFAYKDLQSA 504

Query: 506 TKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVNLVKL 565
           T NFS K+G GGFGSVY G + DGSR+AVKKLE +GQG +EFRAEVS+IG IHH++LV+L
Sbjct: 505 TNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLEGIGQGKKEFRAEVSIIGSIHHLHLVRL 564

Query: 566 TGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECE 625
            GFC+E  HRLL YE++S GSL++WIF  ++    LDWDTRFNIALG A+ +AYLHE+C+
Sbjct: 565 RGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIALGTAKGLAYLHEDCD 624

Query: 626 SKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAI 685
           ++I+HCDIKPEN+LLD NF  KVSDFG+AKLM  ++S + T +RGTRGY+APEWIT+ AI
Sbjct: 625 ARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHVFTTMRGTRGYLAPEWITNYAI 684

Query: 686 SDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRLQ-V 745
           S+K+DVYSYG++LLE+I GRK+Y  D  +  EK H PS+A K + E K   ++D +++ V
Sbjct: 685 SEKSDVYSYGMVLLELIGGRKNY--DPSETSEKCHFPSFAFKKMEEGKLMDIVDGKMKNV 744

Query: 746 EEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWSEMG----SSFSW 805
           +  D RV+ A++ A+WCIQE++  RP M KVVQMLEGV PV +PP  S MG    SSF  
Sbjct: 745 DVTDERVQRAMKTALWCIQEDMQTRPSMSKVVQMLEGVFPVVQPPSSSTMGSRLYSSFFK 804

Query: 806 S---SGAGAMLLGSNGYFSEVRLSDVQLSGPR 820
           S    G      G +   SE  LS V+LSGPR
Sbjct: 805 SISEDGGATTSSGPSDCNSENYLSAVRLSGPR 821

BLAST of MS013081 vs. ExPASy Swiss-Prot
Match: O65238 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 OS=Arabidopsis thaliana OX=3702 GN=At5g35370 PE=2 SV=2)

HSP 1 Score: 427.6 bits (1098), Expect = 3.3e-118
Identity = 293/854 (34.31%), Postives = 434/854 (50.82%), Query Frame = 0

Query: 7   SSFFFSFFLISQSLIVE----SQNIDKILPGFKASASEF-NQTNGNFLLSNNSVFALGFY 66
           S+F     L+S +L+      + +I+ + P F AS   F + + G FLLS NS+F  G +
Sbjct: 3   STFLLLLLLLSLNLLFVFVSCASSIEFVYPNFTASNLRFVDSSKGAFLLSRNSIFKAGLF 62

Query: 67  S-GADDNV--FSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 126
           S G DD+   F   + H+ S   IW++NRD PV+ S        G++ ++  G+++ PVW
Sbjct: 63  SPGGDDSSTGFYFSVVHVDSGSTIWSSNRDSPVSSSGTMNLTPQGISVIE-DGKSQIPVW 122

Query: 127 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 186
           ST      V S++L D+GNL+L  +    +W+SF FPTD+++ GQ    GM L    + +
Sbjct: 123 STPVLASPVKSLRLTDAGNLLLLDHLNVSLWESFDFPTDSIVLGQRLKLGMFLSGSVSRS 182

Query: 187 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNF 246
           D      F  GD     G  +  + W   N  +      A       V +  + ++    
Sbjct: 183 D------FSTGDYKFLVGESDGLMQWRGQNYWKLRMHIRANVDSNFPVEYLTVTTSGLAL 242

Query: 247 YGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPE 306
             + G ++        SS  D  V+ +++ G         GK+   E    P  +C +P 
Sbjct: 243 MARNGTVVVVRVALPPSS--DFRVAKMDSSGKFIVSRFS-GKNLVTE-FSGPMDSCQIPF 302

Query: 307 PCDPLFICYFDN-----RCQCPSILNEKI----------NCKIPSIPCNGSSTELLYLGK 366
            C  L +C  DN      C CP  +              +  +P + C   +   L LG 
Sbjct: 303 VCGKLGLCNLDNASENQSCSCPDEMRMDAGKGVCVPVSQSLSLP-VSCEARNISYLELGL 362

Query: 367 DLDYFALRFSTP-SFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLF-DQIGSLQRSEES 426
            + YF+  F+ P      L +C   C+ NCSC  +F+E  S +C+L  D  GSL   + S
Sbjct: 363 GVSYFSTHFTDPVEHGLPLLACHDICSKNCSCLGVFYENTSRSCYLVKDSFGSLSLVKNS 422

Query: 427 SGGYISYMKTKLPINGGDAEMSPSQYRRRKHI-VLMSILLAAMALAFMGLLCFRFYRRKM 486
              +      KL I   +A+   +  R      V+  +LL       +  L   ++RR  
Sbjct: 423 PENHDLIGYVKLSIRKTNAQPPGNNNRGGSSFPVIALVLLPCSGFFLLIALGLLWWRRCA 482

Query: 487 KELLSSIED--VTEEDKFLE------QISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVY 546
               SSI +  VT    F         I G P +F F +L +AT+NF  +IG GGFGSVY
Sbjct: 483 VMRYSSIREKQVTRPGSFESGDLGSFHIPGLPQKFEFEELEQATENFKMQIGSGGFGSVY 542

Query: 547 LGEVGDGSRVAVKKLERMG-QGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEY 606
            G + D + +AVKK+   G  G +EF  E+++IG I H NLVKL GFC+     LLVYEY
Sbjct: 543 KGTLPDETLIAVKKITNHGLHGRQEFCTEIAIIGNIRHTNLVKLRGFCARGRQLLLVYEY 602

Query: 607 MSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLD 666
           M++GSL+K +F+G  + P L+W  RF+IALG AR +AYLH  C+ KIIHCD+KPEN+LL 
Sbjct: 603 MNHGSLEKTLFSG--NGPVLEWQERFDIALGTARGLAYLHSGCDQKIIHCDVKPENILLH 662

Query: 667 QNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEI 726
            +F PK+SDFG++KL++ ++S + T +RGTRGY+APEWIT+ AIS+K DVYSYG++LLE+
Sbjct: 663 DHFQPKISDFGLSKLLNQEESSLFTTMRGTRGYLAPEWITNAAISEKADVYSYGMVLLEL 722

Query: 727 IAGRK--SYHADSEQPPEK---------------AHLPSYAMKMVAEQKGRRVLDPRLQV 786
           ++GRK  S+ + S    E                 + P YA+ M  + +   + DPRL+ 
Sbjct: 723 VSGRKNCSFRSRSNSVTEDNNQNHSSTTTTSTGLVYFPLYALDMHEQGRYMELADPRLEG 782

Query: 787 EEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWS-----EMGSSFS 804
               +  E  V +A+ C+ EE +LRP M  VV M EG  P+  P + S       G  F+
Sbjct: 783 RVTSQEAEKLVRIALCCVHEEPALRPTMAAVVGMFEGSIPLGNPRMESLNFLRFYGLRFA 838

BLAST of MS013081 vs. ExPASy Swiss-Prot
Match: O64477 (G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabidopsis thaliana OX=3702 GN=At2g19130 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.7e-104
Identity = 288/831 (34.66%), Postives = 422/831 (50.78%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60
           + F  ++SFFF  F I  S  V++ + D  L G        +QT    ++S++  + +GF
Sbjct: 2   VSFLTLTSFFFICFFIHGSSAVDTISGDFTLSG--------DQT----IVSSDGTYEMGF 61

Query: 61  YSGADDNVFSLGITH-IFSSKVIWTANRDFPVND--SALFVFDETGVAYLDGSGRNRAPV 120
           +     + F +G+ +   S  ++W ANRD  V+D  S++F      +  LDG+   + PV
Sbjct: 62  FKPGSSSNFYIGMWYKQLSQTILWVANRDKAVSDKNSSVFKISNGNLILLDGN--YQTPV 121

Query: 121 WSTG---TAGDGVVSMQLLDSGNLVLQSNNGSF----IWQSFHFPTDTLLPG------QV 180
           WSTG   T+    +   L D GNLVL++   S     +WQSF  P DT LPG      + 
Sbjct: 122 WSTGLNSTSSVSALEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDTWLPGVKIRLDKR 181

Query: 181 FWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGG 240
             +  RL S+ +  D S  L     +L  S  Y+   I W  SN+       ++G     
Sbjct: 182 TGKSQRLTSWKSLEDPSPGLFSL--ELDESTAYK---ILWNGSNE-----YWSSGPWNPQ 241

Query: 241 YVLFAILESNSWNFYGQKGELLWEFRFFWQSSWKDRWVS------------VLNTDGSIS 300
             +F  +     N+       ++ F FF  S+  D + +            V++  G I 
Sbjct: 242 SRIFDSVPEMRLNY-------IYNFSFF--SNTTDSYFTYSIYNQLNVSRFVMDVSGQIK 301

Query: 301 FHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNR--CQCPS----ILNEKINCKI 360
                 G  A       P   C V   C    IC   +   C+CP     +  +  + K 
Sbjct: 302 QFTWLEGNKAWNLFWSQPRQQCQVYRYCGSFGICSDKSEPFCRCPQGFRPMSQKDWDLKD 361

Query: 361 PSIPCNGSSTELLYLGKDLDYFALRFSTPSF----------NSDLNSCKAACAGNCSCHV 420
            S  C    TEL     D++ F   F  P+            + L+ C +AC G+CSC  
Sbjct: 362 YSAGC-VRKTELQCSRGDINQF---FRLPNMKLADNSEVLTRTSLSICASACQGDCSCKA 421

Query: 421 LFFEPISGNCFLF--DQIGSLQRSEESSGGYISYMKTKLPINGGDA-EMSPSQYRRRKHI 480
             ++  S  C ++  D +   Q  +E+S G I Y++    +   D   +  S     K +
Sbjct: 422 YAYDEGSSKCLVWSKDVLNLQQLEDENSEGNIFYLR----LAASDVPNVGASGKSNNKGL 481

Query: 481 VLMSIL--LAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQ 540
           +  ++L  L  + L  + ++    YRR+ K +              E+  G    FS+R+
Sbjct: 482 IFGAVLGSLGVIVLVLLVVILILRYRRR-KRMRG------------EKGDGTLSAFSYRE 541

Query: 541 LRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVN 600
           L+ ATKNFS K+G GGFGSV+ G + D S +AVK+LE + QG ++FR EV  IG I HVN
Sbjct: 542 LQNATKNFSDKLGGGGFGSVFKGALPDSSDIAVKRLEGISQGEKQFRTEVVTIGTIQHVN 601

Query: 601 LVKLTGFCSESLHRLLVYEYMSNGSLDKWIF-NGEEDAPFLDWDTRFNIALGIARAVAYL 660
           LV+L GFCSE   +LLVY+YM NGSLD  +F N  E+   L W  RF IALG AR +AYL
Sbjct: 602 LVRLRGFCSEGSKKLLVYDYMPNGSLDSHLFLNQVEEKIVLGWKLRFQIALGTARGLAYL 661

Query: 661 HEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWI 720
           H+EC   IIHCDIKPEN+LLD  F PKV+DFG+AKL+    S + T +RGTRGY+APEWI
Sbjct: 662 HDECRDCIIHCDIKPENILLDSQFCPKVADFGLAKLVGRDFSRVLTTMRGTRGYLAPEWI 721

Query: 721 THLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKG-RRVLD 780
           + +AI+ K DVYSYG++L E+++GR+  + +  +  +    PS+A  ++ +    R ++D
Sbjct: 722 SGVAITAKADVYSYGMMLFELVSGRR--NTEQSENEKVRFFPSWAATILTKDGDIRSLVD 776

BLAST of MS013081 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.0e-104
Identity = 280/837 (33.45%), Postives = 419/837 (50.06%), Query Frame = 0

Query: 10  FFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF--YSGADDN 69
           FF FFL+  SL  E      I  G K  ASE N+      +S N  FA+GF  +   D  
Sbjct: 16  FFCFFLV--SLATEPH----IGLGSKLKASEPNRA----WVSANGTFAIGFTRFKPTDRF 75

Query: 70  VFSLGITHI-FSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWSTGTAGDG 129
           + S+    +     ++W+ NR+ PV   A+   + TG   L  S +N   VW++ T+  G
Sbjct: 76  LLSIWFAQLPGDPTIVWSPNRNSPVTKEAVLELEATGNLVL--SDQNTV-VWTSNTSNHG 135

Query: 130 VVSMQLLDSGNLVL---QSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDLSNF 189
           V S  + +SGN +L   +   G  IWQSF  P+DTLLP Q     + L S P+ +   ++
Sbjct: 136 VESAVMSESGNFLLLGTEVTAGPTIWQSFSQPSDTLLPNQPLTVSLELTSNPSPSRHGHY 195

Query: 190 ---LEFKHGDLVLSAGYR---NPQI---YWA---LSNDSRKIPKSAAGSG---------- 249
              +  +H  L L   Y    +P     YW+   +SN +  +      +G          
Sbjct: 196 SLKMLQQHTSLSLGLTYNINLDPHANYSYWSGPDISNVTGDVTAVLDDTGSFKIVYGESS 255

Query: 250 -GGGYVLFAILE-----SNSWNFYGQKGELL---------------WEFRFFWQSSWKDR 309
            G  YV    ++     +NS N    K  +L               W+      S W   
Sbjct: 256 IGAVYVYKNPVDDNRNYNNSSNLGLTKNPVLRRLVLENNGNLRLYRWDNDMNGSSQWVPE 315

Query: 310 WVSVLN---TDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNRC--QCP 369
           W +V N     G        + ++          G+  +P+  +   +C  ++    +C 
Sbjct: 316 WAAVSNPCDIAGICGNGVCNLDRTKKNADCLCLPGSVKLPDQ-ENAKLCSDNSSLVQECE 375

Query: 370 SILNEKINCKIPSIPCNGSSTELLYLGKDLDYFALRFSTPSFN--SDLNSCKAACAGNCS 429
           S +N   + KI ++       E  Y      YF+ R    + +  S++  C   C  +C 
Sbjct: 376 SNINRNGSFKISTV------QETNY------YFSERSVIENISDISNVRKCGEMCLSDCK 435

Query: 430 C--HVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKL------PINGGDAEMSPS 489
           C   V   +     C++   + SL        G   ++KT+         N  D++   S
Sbjct: 436 CVASVYGLDDEKPYCWI---LKSLNFGGFRDPGSTLFVKTRANESYPSNSNNNDSKSRKS 495

Query: 490 QYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPM 549
              R+K +V+  ++   + +A +G+L +    RK      +++   +    L      P+
Sbjct: 496 HGLRQKVLVIPIVVGMLVLVALLGMLLYYNLDRK-----RTLKRAAKNSLIL---CDSPV 555

Query: 550 RFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLER-MGQGAREFRAEVSLI 609
            F++R L+  T NFS+ +G GGFG+VY G V   + VAVK+L+R +  G REF  EV+ I
Sbjct: 556 SFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDRALSHGEREFITEVNTI 615

Query: 610 GGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIA 669
           G +HH+NLV+L G+CSE  HRLLVYEYM NGSLDKWIF+ E+ A  LDW TRF IA+  A
Sbjct: 616 GSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEIAVATA 675

Query: 670 RAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGY 729
           + +AY HE+C ++IIHCDIKPEN+LLD NF PKVSDFG+AK+M  + S + T +RGTRGY
Sbjct: 676 QGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHVVTMIRGTRGY 735

Query: 730 VAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKG 781
           +APEW+++  I+ K DVYSYG+LLLEI+ GR+  + D     E    P +A K +     
Sbjct: 736 LAPEWVSNRPITVKADVYSYGMLLLEIVGGRR--NLDMSYDAEDFFYPGWAYKELTNGTS 795

BLAST of MS013081 vs. ExPASy Swiss-Prot
Match: Q9XID3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabidopsis thaliana OX=3702 GN=At1g34300 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 9.3e-97
Identity = 244/756 (32.28%), Postives = 386/756 (51.06%), Query Frame = 0

Query: 51  SNNSVFALGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGS 110
           S NS F++ F      N F L       S  IW+A      +  +L +     +   +GS
Sbjct: 44  SPNSTFSVSFVPSPSPNSF-LAAVSFAGSVPIWSAGT--VDSRGSLRLHTSGSLRLTNGS 103

Query: 111 GRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMR 170
           G     VW + T   GV S  + D+G  +L +N    +W SF  PTDT++  Q F  G  
Sbjct: 104 G---TTVWDSKTDRLGVTSGSIEDTGEFILLNNRSVPVWSSFDNPTDTIVQSQNFTAGKI 163

Query: 171 LRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYW-----ALSNDSRKIPKSAAGSGGGGY 230
           LRS      L +F   + G+L L   +    IYW     +  + +   P+ +  + G   
Sbjct: 164 LRS-----GLYSFQLERSGNLTLR--WNTSAIYWNHGLNSSFSSNLSSPRLSLQTNG--- 223

Query: 231 VLFAILESNSWN-----FYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGK 290
            + +I ESN        + G  G+    FRF             L+ DG++  ++     
Sbjct: 224 -VVSIFESNLLGGAEIVYSGDYGDSN-TFRFL-----------KLDDDGNLRIYSSASRN 283

Query: 291 SAPPEPIRIPAGNCGVPEPCDPLFICYFDNR---CQCPS-------ILNEKINC--KIPS 350
           S P          C V   C    IC +++    C CPS       + + +  C  K+  
Sbjct: 284 SGPVNAHWSAVDQCLVYGYCGNFGICSYNDTNPICSCPSRNFDFVDVNDRRKGCKRKVEL 343

Query: 351 IPCNGSSTELLYLGKDLDYFALRFSTPSFNSDLNSCKAACAGNCSCHV-LFFEPISGNCF 410
             C+G++T L  +   L  +    ++ SF +  + C+A C  +  C   +     SGNC+
Sbjct: 344 SDCSGNTTMLDLVHTRLFTYEDDPNSESFFAGSSPCRANCLSSVLCLASVSMSDGSGNCW 403

Query: 411 LFDQIGSLQRSEESSGGYISYMKTKLPINGGDAEMSP--SQYRRRKHIVLMSILLAAMAL 470
                      +  S    SY+K   P+     E +        + H+ ++++ + A  L
Sbjct: 404 QKHPGSFFTGYQWPSVPSTSYVKVCGPVVANTLERATKGDDNNSKVHLWIVAVAVIAGLL 463

Query: 471 AFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGH 530
             + +    ++    K        ++     LE  SG P++F++++L+R TK+F  K+G 
Sbjct: 464 GLVAVEIGLWWCCCRKN--PRFGTLSSHYTLLEYASGAPVQFTYKELQRCTKSFKEKLGA 523

Query: 531 GGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHR 590
           GGFG+VY G + + + VAVK+LE + QG ++FR EV+ I   HH+NLV+L GFCS+  HR
Sbjct: 524 GGFGTVYRGVLTNRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSQGRHR 583

Query: 591 LLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECESKIIHCDIKP 650
           LLVYE+M NGSLD ++F   + A FL W+ RFNIALG A+ + YLHEEC   I+HCDIKP
Sbjct: 584 LLVYEFMRNGSLDNFLFT-TDSAKFLTWEYRFNIALGTAKGITYLHEECRDCIVHCDIKP 643

Query: 651 ENVLLDQNFTPKVSDFGMAKLMDAQKS-FICTQLRGTRGYVAPEWITHLAISDKTDVYSY 710
           EN+L+D NF  KVSDFG+AKL++ + + +  + +RGTRGY+APEW+ +L I+ K+DVYSY
Sbjct: 644 ENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWLANLPITSKSDVYSY 703

Query: 711 GVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRLQVEEA--DRRVE 770
           G++LLE+++G++++    +   +K  +  +A +   +   + +LD RL  ++     +V 
Sbjct: 704 GMVLLELVSGKRNFDVSEKTNHKKFSI--WAYEEFEKGNTKAILDTRLSEDQTVDMEQVM 763

Query: 771 AAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKP 779
             V+ + WCIQE+   RP M KVVQMLEG++ +  P
Sbjct: 764 RMVKTSFWCIQEQPLQRPTMGKVVQMLEGITEIKNP 765

BLAST of MS013081 vs. ExPASy TrEMBL
Match: A0A6J1DHU2 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Momordica charantia OX=3673 GN=LOC111021045 PE=4 SV=1)

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 660/667 (98.95%), Postives = 662/667 (99.25%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60
           MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF
Sbjct: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60

Query: 61  YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST 120
           YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST
Sbjct: 61  YSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWST 120

Query: 121 GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL 180
           GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL
Sbjct: 121 GTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDL 180

Query: 181 SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG 240
           SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG
Sbjct: 181 SNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYG 240

Query: 241 QKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC 300
           QKGELLWEFRFFWQS+WKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC
Sbjct: 241 QKGELLWEFRFFWQSNWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPC 300

Query: 301 DPLFICYFDNRCQCPSILNEKINCKIPSIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD 360
           DPLFICYFDNRCQCPSILNEKINCKI SIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD
Sbjct: 301 DPLFICYFDNRCQCPSILNEKINCKIHSIPCNGSSTELLYLGKDLDYFALRFSTPSFNSD 360

Query: 361 LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA 420
           LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA
Sbjct: 361 LNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDA 420

Query: 421 EMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQI 480
           EMSPSQYRRRKHIVLMSILLAAMALAFMGL CFRFYRRKMKELLSSIE+ TEEDKFLEQI
Sbjct: 421 EMSPSQYRRRKHIVLMSILLAAMALAFMGLFCFRFYRRKMKELLSSIEEATEEDKFLEQI 480

Query: 481 SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE 540
           SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE
Sbjct: 481 SGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAE 540

Query: 541 VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA 600
           VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA
Sbjct: 541 VSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIA 600

Query: 601 LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG 660
           LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG
Sbjct: 601 LGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRG 660

Query: 661 TRGYVAP 668
           TRG V P
Sbjct: 661 TRGLVDP 667

BLAST of MS013081 vs. ExPASy TrEMBL
Match: A0A6J1KDN3 (Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111492904 PE=3 SV=1)

HSP 1 Score: 1308.9 bits (3386), Expect = 0.0e+00
Identity = 650/832 (78.12%), Postives = 728/832 (87.50%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSL--IVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFAL 60
           MG  KI+SFFF F  + QS   IV+SQ IDK+ PGFKASASEFN TNG FLLS  S+F+L
Sbjct: 1   MGLFKIASFFFFFVFLIQSHIDIVKSQEIDKLNPGFKASASEFNDTNGVFLLSKGSIFSL 60

Query: 61  GFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 120
           GFY+GA+D+ FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG AYLD  G N AP+W
Sbjct: 61  GFYAGANDDTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDAYLDQFGPNSAPIW 120

Query: 121 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 180
           ST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY NDN
Sbjct: 121 STETARHGVVSMQLLDSGNLVLKSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLVSYSNDN 180

Query: 181 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILESN 240
           +LS+FLEFK GDLVLSAGY+NPQIYWALS DSRKI ++AAGSG G    GYVLFAI+ESN
Sbjct: 181 NLSSFLEFKQGDLVLSAGYQNPQIYWALSKDSRKIKRAAAGSGSGNGGDGYVLFAIMESN 240

Query: 241 SWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNC 300
           SWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +C
Sbjct: 241 SWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAESC 300

Query: 301 GVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNGSS--TELLYLGKDLDYFALR 360
           GVPEPCDPLFICYFDNRCQCPS I ++K NCK PSI CNG S  TELLYLGK+LDYFALR
Sbjct: 301 GVPEPCDPLFICYFDNRCQCPSTIFDDKFNCKFPSISCNGRSNATELLYLGKNLDYFALR 360

Query: 361 FSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKT 420
           F+ P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK 
Sbjct: 361 FAIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMKI 420

Query: 421 KLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDV 480
           +LPIN  D E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED 
Sbjct: 421 ELPINSNDNETTPTPTPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIEDA 480

Query: 481 TEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERM 540
           TEEDKFLE++S GPMRFS+RQLRRAT+NFS KIG GGFGSVYLGE+GDG+R+AVKKLE++
Sbjct: 481 TEEDKFLEEVSSGPMRFSYRQLRRATRNFSTKIGKGGFGSVYLGELGDGTRLAVKKLEKI 540

Query: 541 GQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPF 600
           GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  F
Sbjct: 541 GQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG-F 600

Query: 601 LDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQ 660
           LDW+TRFNIALG ARA+AYLH+ECESKIIHCDIKPENVLLD NFTPK+SDFGMAKL++ +
Sbjct: 601 LDWETRFNIALGTARALAYLHQECESKIIHCDIKPENVLLDDNFTPKLSDFGMAKLVNRE 660

Query: 661 KSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAH 720
           +S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG++LLEII  RK Y  D++QPPE AH
Sbjct: 661 QSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYGMVLLEIIGNRKCY--DADQPPESAH 720

Query: 721 LPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQM 780
           LPSYA +MVAE+KGR VLDPR+   V E D RVEAAVEVAVWC+QEE S RPPMRKVVQM
Sbjct: 721 LPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEAAVEVAVWCVQEEASFRPPMRKVVQM 780

Query: 781 LEGVSPVPKPPIWSEMGSSFSWSSGAG-AMLLGSNGYFSEVRLSDVQLSGPR 820
           LEGV PVP+PP  +E+G SFSWSSG G  M LG NG FSEVRLSDV+LSGPR
Sbjct: 781 LEGVCPVPRPPSAAEIGWSFSWSSGGGTVMSLGLNGCFSEVRLSDVRLSGPR 829

BLAST of MS013081 vs. ExPASy TrEMBL
Match: A0A6J1G9X7 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452279 PE=3 SV=1)

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 647/833 (77.67%), Postives = 726/833 (87.15%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSL--IVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFAL 60
           MG  KI+SFFF F  + QS   IV+ Q IDK+ PGFKASASE N TNG FLLS  S+FAL
Sbjct: 1   MGLFKIASFFFFFVFLIQSHIDIVKCQEIDKLNPGFKASASELNHTNGVFLLSKASIFAL 60

Query: 61  GFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 120
           GFY+GA+D  FSLGITHI SS+VIWTANRDFPVNDSALFVFDETG  YLD  G N AP+W
Sbjct: 61  GFYAGANDGTFSLGITHILSSRVIWTANRDFPVNDSALFVFDETGDTYLDQFGPNSAPIW 120

Query: 121 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 180
           ST TA  GVVSMQLLDSGNLVL+S NGSF+WQSFHFPT+TLLPGQVFWEGM+L SY NDN
Sbjct: 121 STETARHGVVSMQLLDSGNLVLRSKNGSFVWQSFHFPTNTLLPGQVFWEGMKLESYSNDN 180

Query: 181 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGG----GYVLFAILESN 240
           +LS+FLEFK GDLVLSAGY+NPQ+YWALSND RKI ++AAGSG G    GYVLFAI+ESN
Sbjct: 181 NLSSFLEFKQGDLVLSAGYQNPQVYWALSNDGRKIKRAAAGSGSGNGGDGYVLFAIMESN 240

Query: 241 SWNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNC 300
           SWNF+G+ GELLW F+FFWQS+WKDRWVSVLNTDGSI+FHNLE GKSAPPEPIRIPA +C
Sbjct: 241 SWNFFGKNGELLWGFKFFWQSNWKDRWVSVLNTDGSIAFHNLESGKSAPPEPIRIPAESC 300

Query: 301 GVPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNG--SSTELLYLGKDLDYFALR 360
           GVPEPCDPLFICYFDNRCQC S IL++K NCK PSI CNG  +STELLYLGK+LDYFALR
Sbjct: 301 GVPEPCDPLFICYFDNRCQCASTILDDKFNCKFPSISCNGRSNSTELLYLGKNLDYFALR 360

Query: 361 FSTPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKT 420
           FS P+ NSDLN+CKAACA NCSC+V+FFEP SG+CF FD+IGSLQRS+E S GYISYMK 
Sbjct: 361 FSIPALNSDLNNCKAACASNCSCNVMFFEPNSGDCFFFDEIGSLQRSDEGSNGYISYMKI 420

Query: 421 KLPINGGDAEMSPSQY-RRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDV 480
           +LPIN  + E +P+    RRKHIVLMSIL+AAMAL+FMGLLCF FYRRK+KELLSSIED 
Sbjct: 421 ELPINSNNNETTPTPTPNRRKHIVLMSILIAAMALSFMGLLCFLFYRRKVKELLSSIEDA 480

Query: 481 TEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERM 540
           TEEDKFLE++S GPMRFS+RQLRRAT+NFS KIGHGGFGSVYLG++GDG+R+AVKKLE++
Sbjct: 481 TEEDKFLEEVSSGPMRFSYRQLRRATRNFSTKIGHGGFGSVYLGDLGDGTRLAVKKLEKI 540

Query: 541 GQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPF 600
           GQG REFRAEVSLIGGIHHVNLVKL GFCSE+LHRLLVYEYMSNGSLDKWIFNG+ED  F
Sbjct: 541 GQGGREFRAEVSLIGGIHHVNLVKLKGFCSENLHRLLVYEYMSNGSLDKWIFNGKEDG-F 600

Query: 601 LDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQ 660
           LDW+TRFNIALG ARA+AYLH+ECESKIIHCDIKPENVLLD NFTPK+SDFGMAKL++ +
Sbjct: 601 LDWETRFNIALGTARALAYLHQECESKIIHCDIKPENVLLDDNFTPKLSDFGMAKLVNRE 660

Query: 661 KSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAH 720
           +S I TQLRGTRGY+APEWIT+LAISDK+DVYSYG++LLEI+A RK Y  D++Q PE AH
Sbjct: 661 QSNIFTQLRGTRGYMAPEWITNLAISDKSDVYSYGMVLLEIVANRKCY--DADQSPESAH 720

Query: 721 LPSYAMKMVAEQKGRRVLDPRL--QVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQM 780
           LPSYA +MVAE+KGR VLDPR+   V E D RVEA VEVAVWC+QEE SLRPPMRKVVQM
Sbjct: 721 LPSYAARMVAEKKGRWVLDPRVAATVTEEDWRVEAVVEVAVWCVQEEASLRPPMRKVVQM 780

Query: 781 LEGVSPVPKPPIWSEMGSSFSWSSGAG--AMLLGSNGYFSEVRLSDVQLSGPR 820
           LEGV PVP+PP  +EMG SFSWSSG G   M LG NG FSEVRLSDV+LSGPR
Sbjct: 781 LEGVCPVPRPPSAAEMGRSFSWSSGGGGTVMSLGLNGCFSEVRLSDVRLSGPR 830

BLAST of MS013081 vs. ExPASy TrEMBL
Match: A0A0A0KTK3 (Receptor-like serine/threonine-protein kinase OS=Cucumis sativus OX=3659 GN=Csa_4G005510 PE=3 SV=1)

HSP 1 Score: 1265.4 bits (3273), Expect = 0.0e+00
Identity = 631/826 (76.39%), Postives = 711/826 (86.08%), Query Frame = 0

Query: 1   MGFHKISSFFFSF---FLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFA 60
           MGF KI++F F F   F+ + + IV+SQ+ID+I PGF+ASASEFN TNG FLLS  SVFA
Sbjct: 1   MGFFKIATFLFVFSLLFIQTNTAIVKSQSIDQINPGFRASASEFNHTNGVFLLSKRSVFA 60

Query: 61  LGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPV 120
           LGFY+GA DN FSLGI HIFSS+VIWTANRD  VNDSA FVF+ETG AYLD SG+N+  V
Sbjct: 61  LGFYAGAKDNTFSLGIIHIFSSRVIWTANRDSLVNDSAFFVFNETGDAYLDVSGQNQTTV 120

Query: 121 WSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPND 180
           WST TA +GVVSMQLLDSGNLVL+S NGSFIWQSFHFPTDTLLPGQ+FWEG++L+SYPND
Sbjct: 121 WSTETANEGVVSMQLLDSGNLVLKSKNGSFIWQSFHFPTDTLLPGQIFWEGLKLKSYPND 180

Query: 181 NDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWN 240
           ND SNFLEFK GDLVLSAGY+NPQIYWALSNDSRKI +  A +GG GY LFAILESN WN
Sbjct: 181 NDHSNFLEFKQGDLVLSAGYQNPQIYWALSNDSRKIQR--ATTGGSGYSLFAILESNYWN 240

Query: 241 FYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVP 300
           FYG  GELLW F+ FWQ + KDRW+SVLNTDG+ISF NLE  KSA PEPIRIPA  CGVP
Sbjct: 241 FYGTNGELLWSFKIFWQLNRKDRWISVLNTDGTISFLNLENRKSAEPEPIRIPAEICGVP 300

Query: 301 EPCDPLFICYFDNRCQCPSILNEK-INCKIPSIPCNGS--STELLYLGKDLDYFALRFST 360
           EPC+PLFICYFDN CQCPS + EK  NCK+PS+PCNGS  STELLYLG++LDYFALRFST
Sbjct: 301 EPCNPLFICYFDNHCQCPSTVFEKNFNCKLPSVPCNGSSNSTELLYLGENLDYFALRFST 360

Query: 361 PSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLP 420
           P+FNSDL+SCK AC+ NCSC+V+F+EP+S NC+ F++IGS +RSE  SGGYISYMKT LP
Sbjct: 361 PAFNSDLSSCKTACSSNCSCNVMFYEPVSRNCYFFNEIGSFRRSEGGSGGYISYMKTNLP 420

Query: 421 INGGDAEMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEED 480
           ING ++E +PS   RRKHIVLMS+L+AAM L FMGLLCF FYR+KMKELLSSI++ TEED
Sbjct: 421 INGNNSETNPSP-NRRKHIVLMSLLMAAMTLGFMGLLCFLFYRQKMKELLSSIDEATEED 480

Query: 481 KFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGA 540
            FL +ISGGP+R+S+RQLRRATKNFS KIG GGFGSVYLG++GDGSR+AVKKLER+GQG 
Sbjct: 481 IFLNEISGGPIRYSYRQLRRATKNFSTKIGDGGFGSVYLGKMGDGSRLAVKKLERIGQGG 540

Query: 541 REFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWD 600
           REFRAEVSLIGGIHHVNLVKL GFCSESLHRLLVYEYMSNGSLDKWIFN +ED  FLDW 
Sbjct: 541 REFRAEVSLIGGIHHVNLVKLKGFCSESLHRLLVYEYMSNGSLDKWIFNKKEDDLFLDWS 600

Query: 601 TRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFI 660
           TRFNIALG  RA+AYLH+ECESKIIHCDIKPEN+LLD+NFTPK+SDFGMAKLMD Q + I
Sbjct: 601 TRFNIALGTGRALAYLHQECESKIIHCDIKPENILLDENFTPKLSDFGMAKLMDKQHTSI 660

Query: 661 CTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSY 720
            TQLRGTRGYVAPEWIT LAISDK+DVYSYG+LLLEIIAGRKSY  D++ PPE AHLPSY
Sbjct: 661 FTQLRGTRGYVAPEWITTLAISDKSDVYSYGMLLLEIIAGRKSY--DADYPPEMAHLPSY 720

Query: 721 AMKMVAEQKGRRVLDPRLQVE-EADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVS 780
           A +MV EQKG RVLD R+  E E D RVEAAV+VAVWC+QEE SLRPPMRKVVQMLEGVS
Sbjct: 721 ATRMVGEQKGFRVLDSRVAGEAEGDWRVEAAVQVAVWCVQEEPSLRPPMRKVVQMLEGVS 780

Query: 781 PVPKPPIWSEMGSSFSWSSGAGAMLLGSNGYFSEVRLSDVQLSGPR 820
           PVP PP  +EMG++F WSS    M L  NG +SEVRLSDV+LSGPR
Sbjct: 781 PVPMPPCTAEMGANFWWSSDGLGMKL--NGCYSEVRLSDVRLSGPR 819

BLAST of MS013081 vs. ExPASy TrEMBL
Match: A0A5D3E1U3 (Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001770 PE=3 SV=1)

HSP 1 Score: 1258.4 bits (3255), Expect = 0.0e+00
Identity = 631/828 (76.21%), Postives = 711/828 (85.87%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLI-----SQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSV 60
           MGF KI+SF F FF +     + + IV+SQ ID+I PGF+ASASEFN TNG FLLS +SV
Sbjct: 1   MGFFKIASFLFFFFSLHLIIQTNTAIVKSQRIDQINPGFRASASEFNHTNGVFLLSKSSV 60

Query: 61  FALGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRA 120
           FALGFY+GA+DN FSLGI HI SS+VIWTANRD  VNDSA FVF+ETG AYLD SG+N+ 
Sbjct: 61  FALGFYAGANDNTFSLGIIHISSSRVIWTANRDSLVNDSAFFVFNETGDAYLDVSGQNQT 120

Query: 121 PVWSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYP 180
            VWST TA +GV+SMQLLDSGNLVL+S NGSFIWQSFHFPTDTL+PGQVFWEG++L+SYP
Sbjct: 121 TVWSTETAHEGVLSMQLLDSGNLVLKSKNGSFIWQSFHFPTDTLMPGQVFWEGLKLKSYP 180

Query: 181 NDNDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNS 240
           NDNDLSNFLEFK GDLVLSAGY+NPQIYWALSNDSRKI +  A +GG GYVLFAILESN 
Sbjct: 181 NDNDLSNFLEFKQGDLVLSAGYQNPQIYWALSNDSRKIQR--ATTGGSGYVLFAILESNY 240

Query: 241 WNFYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCG 300
           WNFYG +GELLW F+ FW  + KDRW+SVLNTDG+ISF NLE  KSA PE IRIPA  CG
Sbjct: 241 WNFYGMEGELLWSFKIFWHFNRKDRWISVLNTDGTISFLNLENRKSAEPETIRIPAEICG 300

Query: 301 VPEPCDPLFICYFDNRCQCPS-ILNEKINCKIPSIPCNGS--STELLYLGKDLDYFALRF 360
           VPEPC+PLFICYFDN CQCPS IL++  NCK+PSIPCNGS  ST+LLYLG++LDYFALRF
Sbjct: 301 VPEPCNPLFICYFDNHCQCPSTILDKNFNCKLPSIPCNGSSNSTKLLYLGENLDYFALRF 360

Query: 361 STPSFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTK 420
           STPSFNSDL+SCK AC  NCSC+V+F+EP+S NC+ F++IGSLQRSE  SGGYISYMKT 
Sbjct: 361 STPSFNSDLSSCKTACFSNCSCNVMFYEPVSRNCYFFNEIGSLQRSEGGSGGYISYMKTN 420

Query: 421 LPINGGDAEMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTE 480
           LPING ++E +PS   RRKHIVLMS+L+AAMAL FMGLLCF FYR+KMKELLSSI++ TE
Sbjct: 421 LPINGNNSETNPSP-NRRKHIVLMSLLMAAMALGFMGLLCFLFYRQKMKELLSSIDEATE 480

Query: 481 EDKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQ 540
           EDKFL +ISG PMR+S+RQLRRATKNFS KIG GGFGSVYLG++GDGSR+AVKKLER+GQ
Sbjct: 481 EDKFLNEISGAPMRYSYRQLRRATKNFSTKIGDGGFGSVYLGKMGDGSRLAVKKLERIGQ 540

Query: 541 GAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLD 600
           G REF+AEVSLIGGIHHVNLVKL GFCSESLHRLLVYEYMSNGSLDKWIFN +ED   LD
Sbjct: 541 GGREFQAEVSLIGGIHHVNLVKLKGFCSESLHRLLVYEYMSNGSLDKWIFNKKEDDLLLD 600

Query: 601 WDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKS 660
           W+TRF+IALG  RA+AYLH+ECESKIIHCDIKPEN+LLD+NFTPK+SDFGMAK MD Q +
Sbjct: 601 WNTRFSIALGTGRALAYLHQECESKIIHCDIKPENILLDENFTPKLSDFGMAKSMDKQHT 660

Query: 661 FICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLP 720
            I TQLRGTRGYVAPEWIT LAISDK+DVYSYG+LLLEIIAGRKSY  D + PPE AHLP
Sbjct: 661 SIFTQLRGTRGYVAPEWITTLAISDKSDVYSYGMLLLEIIAGRKSY--DPDYPPEMAHLP 720

Query: 721 SYAMKMVAEQKGRRVLDPRLQVE-EADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEG 780
           SYA +MVAEQKG RVLD R+  E E D RVEAAV+VAVWC+QEE SLRPPMRKVVQMLEG
Sbjct: 721 SYATRMVAEQKGFRVLDSRVAGEAEGDWRVEAAVQVAVWCVQEEPSLRPPMRKVVQMLEG 780

Query: 781 VSPVPKPPIWSEMGSSFSWSSGAGAMLLGSNGYFSEVRLSDVQLSGPR 820
           V PVP PP  +EMG+S  WS+    M L  NG FSEVRLSDV+LSGPR
Sbjct: 781 VCPVPMPPCTAEMGASLWWSNEGLGMNL--NGCFSEVRLSDVRLSGPR 821

BLAST of MS013081 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 703.7 bits (1815), Expect = 1.7e-202
Identity = 377/812 (46.43%), Postives = 520/812 (64.04%), Query Frame = 0

Query: 26  NIDKILPGFKASASEFNQTNGNFLLSNNSVFALGFYSGADD-NVFSLGITHIFSSKVIWT 85
           +I  I PGF  S   +   +G FL SNNS F  GF +  D   +F+L I H  S+K+IW+
Sbjct: 25  SIGSITPGFGGSQMNYINNDGIFLESNNSAFGFGFVTTQDSVTLFTLSIIHKSSTKLIWS 84

Query: 86  ANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNN 145
           ANR  PV++S  FVFD+ G   ++G+      VW    +G     ++L DSGNLV+ S +
Sbjct: 85  ANRASPVSNSDKFVFDDNGNVVMEGT-----EVWRLDNSGKNASRIELRDSGNLVVVSVD 144

Query: 146 GSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYW 205
           G+ IW+SF  PTDTL+  Q F EGM+L S P+ ++++  LE K GD+VLS     PQ+YW
Sbjct: 145 GTSIWESFDHPTDTLITNQAFKEGMKLTSSPSSSNMTYALEIKSGDMVLSVNSLTPQVYW 204

Query: 206 ALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNFYGQKGELLWEFRFFWQSSWKDRWVSV 265
           +++N   +I         GG V  + L  NSW F+ QK  LLW+F F         W++V
Sbjct: 205 SMANARERIINK-----DGGVVTSSSLLGNSWRFFDQKQVLLWQFVFSDNKDDNTTWIAV 264

Query: 266 LNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNRCQCPSILNE-KIN 325
           L  +G ISF NL  G SA     +IP+  CG PEPC P ++C     C C S L+  + +
Sbjct: 265 LGNNGVISFSNLGSGASAADSSTKIPSDLCGTPEPCGPYYVCSGSKVCGCVSGLSRARSD 324

Query: 326 CKIP-SIPCNGSS------TELLYLGKDLDYFALRFSTP-SFNSDLNSCKAACAGNCSCH 385
           CK   + PC  +        +L+  G  +DYFAL ++ P S  +DL+SCK  C  NCSC 
Sbjct: 325 CKTGITSPCKKTKDNATLPLQLVSAGDGVDYFALGYAPPFSKKTDLDSCKEFCHNNCSCL 384

Query: 386 VLFFEPISGNCFLFDQIGSLQRSEESSGGYISYMKTKLPINGGDAEMSPSQYRRRKHIVL 445
            LFF+  SGNCFLFD IGS + S     G++SY+K     +GG  +      +   ++V+
Sbjct: 385 GLFFQNSSGNCFLFDYIGSFKTSGNGGSGFVSYIKIASTGSGG-GDNGEDDGKHFPYVVI 444

Query: 446 MSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQLRRA 505
           + +++    +A +  + FR ++RK K +L + ++ +EED FLE +SG P+RF+++ L+ A
Sbjct: 445 I-VVVTVFIIAVLIFVAFRIHKRK-KMILEAPQESSEEDNFLENLSGMPIRFAYKDLQSA 504

Query: 506 TKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVNLVKL 565
           T NFS K+G GGFGSVY G + DGSR+AVKKLE +GQG +EFRAEVS+IG IHH++LV+L
Sbjct: 505 TNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLEGIGQGKKEFRAEVSIIGSIHHLHLVRL 564

Query: 566 TGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECE 625
            GFC+E  HRLL YE++S GSL++WIF  ++    LDWDTRFNIALG A+ +AYLHE+C+
Sbjct: 565 RGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIALGTAKGLAYLHEDCD 624

Query: 626 SKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAI 685
           ++I+HCDIKPEN+LLD NF  KVSDFG+AKLM  ++S + T +RGTRGY+APEWIT+ AI
Sbjct: 625 ARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHVFTTMRGTRGYLAPEWITNYAI 684

Query: 686 SDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRLQ-V 745
           S+K+DVYSYG++LLE+I GRK+Y  D  +  EK H PS+A K + E K   ++D +++ V
Sbjct: 685 SEKSDVYSYGMVLLELIGGRKNY--DPSETSEKCHFPSFAFKKMEEGKLMDIVDGKMKNV 744

Query: 746 EEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWSEMG----SSFSW 805
           +  D RV+ A++ A+WCIQE++  RP M KVVQMLEGV PV +PP  S MG    SSF  
Sbjct: 745 DVTDERVQRAMKTALWCIQEDMQTRPSMSKVVQMLEGVFPVVQPPSSSTMGSRLYSSFFK 804

Query: 806 S---SGAGAMLLGSNGYFSEVRLSDVQLSGPR 820
           S    G      G +   SE  LS V+LSGPR
Sbjct: 805 SISEDGGATTSSGPSDCNSENYLSAVRLSGPR 821

BLAST of MS013081 vs. TAIR 10
Match: AT5G35370.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 427.6 bits (1098), Expect = 2.3e-119
Identity = 293/854 (34.31%), Postives = 434/854 (50.82%), Query Frame = 0

Query: 7   SSFFFSFFLISQSLIVE----SQNIDKILPGFKASASEF-NQTNGNFLLSNNSVFALGFY 66
           S+F     L+S +L+      + +I+ + P F AS   F + + G FLLS NS+F  G +
Sbjct: 3   STFLLLLLLLSLNLLFVFVSCASSIEFVYPNFTASNLRFVDSSKGAFLLSRNSIFKAGLF 62

Query: 67  S-GADDNV--FSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGSGRNRAPVW 126
           S G DD+   F   + H+ S   IW++NRD PV+ S        G++ ++  G+++ PVW
Sbjct: 63  SPGGDDSSTGFYFSVVHVDSGSTIWSSNRDSPVSSSGTMNLTPQGISVIE-DGKSQIPVW 122

Query: 127 STGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMRLRSYPNDN 186
           ST      V S++L D+GNL+L  +    +W+SF FPTD+++ GQ    GM L    + +
Sbjct: 123 STPVLASPVKSLRLTDAGNLLLLDHLNVSLWESFDFPTDSIVLGQRLKLGMFLSGSVSRS 182

Query: 187 DLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGGYVLFAILESNSWNF 246
           D      F  GD     G  +  + W   N  +      A       V +  + ++    
Sbjct: 183 D------FSTGDYKFLVGESDGLMQWRGQNYWKLRMHIRANVDSNFPVEYLTVTTSGLAL 242

Query: 247 YGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPE 306
             + G ++        SS  D  V+ +++ G         GK+   E    P  +C +P 
Sbjct: 243 MARNGTVVVVRVALPPSS--DFRVAKMDSSGKFIVSRFS-GKNLVTE-FSGPMDSCQIPF 302

Query: 307 PCDPLFICYFDN-----RCQCPSILNEKI----------NCKIPSIPCNGSSTELLYLGK 366
            C  L +C  DN      C CP  +              +  +P + C   +   L LG 
Sbjct: 303 VCGKLGLCNLDNASENQSCSCPDEMRMDAGKGVCVPVSQSLSLP-VSCEARNISYLELGL 362

Query: 367 DLDYFALRFSTP-SFNSDLNSCKAACAGNCSCHVLFFEPISGNCFLF-DQIGSLQRSEES 426
            + YF+  F+ P      L +C   C+ NCSC  +F+E  S +C+L  D  GSL   + S
Sbjct: 363 GVSYFSTHFTDPVEHGLPLLACHDICSKNCSCLGVFYENTSRSCYLVKDSFGSLSLVKNS 422

Query: 427 SGGYISYMKTKLPINGGDAEMSPSQYRRRKHI-VLMSILLAAMALAFMGLLCFRFYRRKM 486
              +      KL I   +A+   +  R      V+  +LL       +  L   ++RR  
Sbjct: 423 PENHDLIGYVKLSIRKTNAQPPGNNNRGGSSFPVIALVLLPCSGFFLLIALGLLWWRRCA 482

Query: 487 KELLSSIED--VTEEDKFLE------QISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVY 546
               SSI +  VT    F         I G P +F F +L +AT+NF  +IG GGFGSVY
Sbjct: 483 VMRYSSIREKQVTRPGSFESGDLGSFHIPGLPQKFEFEELEQATENFKMQIGSGGFGSVY 542

Query: 547 LGEVGDGSRVAVKKLERMG-QGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEY 606
            G + D + +AVKK+   G  G +EF  E+++IG I H NLVKL GFC+     LLVYEY
Sbjct: 543 KGTLPDETLIAVKKITNHGLHGRQEFCTEIAIIGNIRHTNLVKLRGFCARGRQLLLVYEY 602

Query: 607 MSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLD 666
           M++GSL+K +F+G  + P L+W  RF+IALG AR +AYLH  C+ KIIHCD+KPEN+LL 
Sbjct: 603 MNHGSLEKTLFSG--NGPVLEWQERFDIALGTARGLAYLHSGCDQKIIHCDVKPENILLH 662

Query: 667 QNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEI 726
            +F PK+SDFG++KL++ ++S + T +RGTRGY+APEWIT+ AIS+K DVYSYG++LLE+
Sbjct: 663 DHFQPKISDFGLSKLLNQEESSLFTTMRGTRGYLAPEWITNAAISEKADVYSYGMVLLEL 722

Query: 727 IAGRK--SYHADSEQPPEK---------------AHLPSYAMKMVAEQKGRRVLDPRLQV 786
           ++GRK  S+ + S    E                 + P YA+ M  + +   + DPRL+ 
Sbjct: 723 VSGRKNCSFRSRSNSVTEDNNQNHSSTTTTSTGLVYFPLYALDMHEQGRYMELADPRLEG 782

Query: 787 EEADRRVEAAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKPPIWS-----EMGSSFS 804
               +  E  V +A+ C+ EE +LRP M  VV M EG  P+  P + S       G  F+
Sbjct: 783 RVTSQEAEKLVRIALCCVHEEPALRPTMAAVVGMFEGSIPLGNPRMESLNFLRFYGLRFA 838

BLAST of MS013081 vs. TAIR 10
Match: AT2G19130.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.9e-105
Identity = 288/831 (34.66%), Postives = 422/831 (50.78%), Query Frame = 0

Query: 1   MGFHKISSFFFSFFLISQSLIVESQNIDKILPGFKASASEFNQTNGNFLLSNNSVFALGF 60
           + F  ++SFFF  F I  S  V++ + D  L G        +QT    ++S++  + +GF
Sbjct: 2   VSFLTLTSFFFICFFIHGSSAVDTISGDFTLSG--------DQT----IVSSDGTYEMGF 61

Query: 61  YSGADDNVFSLGITH-IFSSKVIWTANRDFPVND--SALFVFDETGVAYLDGSGRNRAPV 120
           +     + F +G+ +   S  ++W ANRD  V+D  S++F      +  LDG+   + PV
Sbjct: 62  FKPGSSSNFYIGMWYKQLSQTILWVANRDKAVSDKNSSVFKISNGNLILLDGN--YQTPV 121

Query: 121 WSTG---TAGDGVVSMQLLDSGNLVLQSNNGSF----IWQSFHFPTDTLLPG------QV 180
           WSTG   T+    +   L D GNLVL++   S     +WQSF  P DT LPG      + 
Sbjct: 122 WSTGLNSTSSVSALEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDTWLPGVKIRLDKR 181

Query: 181 FWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDSRKIPKSAAGSGGGG 240
             +  RL S+ +  D S  L     +L  S  Y+   I W  SN+       ++G     
Sbjct: 182 TGKSQRLTSWKSLEDPSPGLFSL--ELDESTAYK---ILWNGSNE-----YWSSGPWNPQ 241

Query: 241 YVLFAILESNSWNFYGQKGELLWEFRFFWQSSWKDRWVS------------VLNTDGSIS 300
             +F  +     N+       ++ F FF  S+  D + +            V++  G I 
Sbjct: 242 SRIFDSVPEMRLNY-------IYNFSFF--SNTTDSYFTYSIYNQLNVSRFVMDVSGQIK 301

Query: 301 FHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFICYFDNR--CQCPS----ILNEKINCKI 360
                 G  A       P   C V   C    IC   +   C+CP     +  +  + K 
Sbjct: 302 QFTWLEGNKAWNLFWSQPRQQCQVYRYCGSFGICSDKSEPFCRCPQGFRPMSQKDWDLKD 361

Query: 361 PSIPCNGSSTELLYLGKDLDYFALRFSTPSF----------NSDLNSCKAACAGNCSCHV 420
            S  C    TEL     D++ F   F  P+            + L+ C +AC G+CSC  
Sbjct: 362 YSAGC-VRKTELQCSRGDINQF---FRLPNMKLADNSEVLTRTSLSICASACQGDCSCKA 421

Query: 421 LFFEPISGNCFLF--DQIGSLQRSEESSGGYISYMKTKLPINGGDA-EMSPSQYRRRKHI 480
             ++  S  C ++  D +   Q  +E+S G I Y++    +   D   +  S     K +
Sbjct: 422 YAYDEGSSKCLVWSKDVLNLQQLEDENSEGNIFYLR----LAASDVPNVGASGKSNNKGL 481

Query: 481 VLMSIL--LAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQ 540
           +  ++L  L  + L  + ++    YRR+ K +              E+  G    FS+R+
Sbjct: 482 IFGAVLGSLGVIVLVLLVVILILRYRRR-KRMRG------------EKGDGTLSAFSYRE 541

Query: 541 LRRATKNFSRKIGHGGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVN 600
           L+ ATKNFS K+G GGFGSV+ G + D S +AVK+LE + QG ++FR EV  IG I HVN
Sbjct: 542 LQNATKNFSDKLGGGGFGSVFKGALPDSSDIAVKRLEGISQGEKQFRTEVVTIGTIQHVN 601

Query: 601 LVKLTGFCSESLHRLLVYEYMSNGSLDKWIF-NGEEDAPFLDWDTRFNIALGIARAVAYL 660
           LV+L GFCSE   +LLVY+YM NGSLD  +F N  E+   L W  RF IALG AR +AYL
Sbjct: 602 LVRLRGFCSEGSKKLLVYDYMPNGSLDSHLFLNQVEEKIVLGWKLRFQIALGTARGLAYL 661

Query: 661 HEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKSFICTQLRGTRGYVAPEWI 720
           H+EC   IIHCDIKPEN+LLD  F PKV+DFG+AKL+    S + T +RGTRGY+APEWI
Sbjct: 662 HDECRDCIIHCDIKPENILLDSQFCPKVADFGLAKLVGRDFSRVLTTMRGTRGYLAPEWI 721

Query: 721 THLAISDKTDVYSYGVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKG-RRVLD 780
           + +AI+ K DVYSYG++L E+++GR+  + +  +  +    PS+A  ++ +    R ++D
Sbjct: 722 SGVAITAKADVYSYGMMLFELVSGRR--NTEQSENEKVRFFPSWAATILTKDGDIRSLVD 776

BLAST of MS013081 vs. TAIR 10
Match: AT1G34300.1 (lectin protein kinase family protein )

HSP 1 Score: 356.3 bits (913), Expect = 6.6e-98
Identity = 244/756 (32.28%), Postives = 386/756 (51.06%), Query Frame = 0

Query: 51  SNNSVFALGFYSGADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFVFDETGVAYLDGS 110
           S NS F++ F      N F L       S  IW+A      +  +L +     +   +GS
Sbjct: 44  SPNSTFSVSFVPSPSPNSF-LAAVSFAGSVPIWSAGT--VDSRGSLRLHTSGSLRLTNGS 103

Query: 111 GRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDTLLPGQVFWEGMR 170
           G     VW + T   GV S  + D+G  +L +N    +W SF  PTDT++  Q F  G  
Sbjct: 104 G---TTVWDSKTDRLGVTSGSIEDTGEFILLNNRSVPVWSSFDNPTDTIVQSQNFTAGKI 163

Query: 171 LRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYW-----ALSNDSRKIPKSAAGSGGGGY 230
           LRS      L +F   + G+L L   +    IYW     +  + +   P+ +  + G   
Sbjct: 164 LRS-----GLYSFQLERSGNLTLR--WNTSAIYWNHGLNSSFSSNLSSPRLSLQTNG--- 223

Query: 231 VLFAILESNSWN-----FYGQKGELLWEFRFFWQSSWKDRWVSVLNTDGSISFHNLEMGK 290
            + +I ESN        + G  G+    FRF             L+ DG++  ++     
Sbjct: 224 -VVSIFESNLLGGAEIVYSGDYGDSN-TFRFL-----------KLDDDGNLRIYSSASRN 283

Query: 291 SAPPEPIRIPAGNCGVPEPCDPLFICYFDNR---CQCPS-------ILNEKINC--KIPS 350
           S P          C V   C    IC +++    C CPS       + + +  C  K+  
Sbjct: 284 SGPVNAHWSAVDQCLVYGYCGNFGICSYNDTNPICSCPSRNFDFVDVNDRRKGCKRKVEL 343

Query: 351 IPCNGSSTELLYLGKDLDYFALRFSTPSFNSDLNSCKAACAGNCSCHV-LFFEPISGNCF 410
             C+G++T L  +   L  +    ++ SF +  + C+A C  +  C   +     SGNC+
Sbjct: 344 SDCSGNTTMLDLVHTRLFTYEDDPNSESFFAGSSPCRANCLSSVLCLASVSMSDGSGNCW 403

Query: 411 LFDQIGSLQRSEESSGGYISYMKTKLPINGGDAEMSP--SQYRRRKHIVLMSILLAAMAL 470
                      +  S    SY+K   P+     E +        + H+ ++++ + A  L
Sbjct: 404 QKHPGSFFTGYQWPSVPSTSYVKVCGPVVANTLERATKGDDNNSKVHLWIVAVAVIAGLL 463

Query: 471 AFMGLLCFRFYRRKMKELLSSIEDVTEEDKFLEQISGGPMRFSFRQLRRATKNFSRKIGH 530
             + +    ++    K        ++     LE  SG P++F++++L+R TK+F  K+G 
Sbjct: 464 GLVAVEIGLWWCCCRKN--PRFGTLSSHYTLLEYASGAPVQFTYKELQRCTKSFKEKLGA 523

Query: 531 GGFGSVYLGEVGDGSRVAVKKLERMGQGAREFRAEVSLIGGIHHVNLVKLTGFCSESLHR 590
           GGFG+VY G + + + VAVK+LE + QG ++FR EV+ I   HH+NLV+L GFCS+  HR
Sbjct: 524 GGFGTVYRGVLTNRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSQGRHR 583

Query: 591 LLVYEYMSNGSLDKWIFNGEEDAPFLDWDTRFNIALGIARAVAYLHEECESKIIHCDIKP 650
           LLVYE+M NGSLD ++F   + A FL W+ RFNIALG A+ + YLHEEC   I+HCDIKP
Sbjct: 584 LLVYEFMRNGSLDNFLFT-TDSAKFLTWEYRFNIALGTAKGITYLHEECRDCIVHCDIKP 643

Query: 651 ENVLLDQNFTPKVSDFGMAKLMDAQKS-FICTQLRGTRGYVAPEWITHLAISDKTDVYSY 710
           EN+L+D NF  KVSDFG+AKL++ + + +  + +RGTRGY+APEW+ +L I+ K+DVYSY
Sbjct: 644 ENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWLANLPITSKSDVYSY 703

Query: 711 GVLLLEIIAGRKSYHADSEQPPEKAHLPSYAMKMVAEQKGRRVLDPRLQVEEA--DRRVE 770
           G++LLE+++G++++    +   +K  +  +A +   +   + +LD RL  ++     +V 
Sbjct: 704 GMVLLELVSGKRNFDVSEKTNHKKFSI--WAYEEFEKGNTKAILDTRLSEDQTVDMEQVM 763

Query: 771 AAVEVAVWCIQEEVSLRPPMRKVVQMLEGVSPVPKP 779
             V+ + WCIQE+   RP M KVVQMLEG++ +  P
Sbjct: 764 RMVKTSFWCIQEQPLQRPTMGKVVQMLEGITEIKNP 765

BLAST of MS013081 vs. TAIR 10
Match: AT4G00340.1 (receptor-like protein kinase 4 )

HSP 1 Score: 342.4 bits (877), Expect = 9.8e-94
Identity = 268/793 (33.80%), Postives = 369/793 (46.53%), Query Frame = 0

Query: 42  NQTNGNFLLSNNSVFALGFYS---GADDNVFSLGITHIFSSKVIWTANRDFPVNDSALFV 101
           NQT    +LS  ++F LGF+S   G+ +    +    + +   +W ANR  PV+D     
Sbjct: 30  NQT----ILSFKAIFRLGFFSTTNGSSNWYLGISYASMPTPTHVWVANRIRPVSDPDSST 89

Query: 102 FDETGVAYLDGSGRNRAPVWSTGTAGDGVVSMQLLDSGNLVLQSNNGSFIWQSFHFPTDT 161
            + T   YL  S      VW T     G    +  ++GNL+L +++GS +WQSF  PTDT
Sbjct: 90  LELTSTGYLIVSNLRDGVVWQTDNKQPG-TDFRFSETGNLILINDDGSPVWQSFDNPTDT 149

Query: 162 LLPG--------QVFWEGMRLRSYPNDNDLSNFLEFKHGDLVLSAGYRNPQIYWALSNDS 221
            LPG           W  +     P    L     F    LV    Y+    YW+  N +
Sbjct: 150 WLPGMNVTGLTAMTSWRSL-FDPSPGFYSLRLSPSFNEFQLV----YKGTTPYWSTGNWT 209

Query: 222 RK----IPKSA-------------AGSGGGGYV---LFAILESNSWNF-YGQKGELLWEF 281
            +    +P+                 +    Y+   L ++ E     F  G  G+L    
Sbjct: 210 GEAFVGVPEMTIPYIYRFHFVNPYTPTASFWYIVPPLDSVSEPRLTRFMVGANGQLK--- 269

Query: 282 RFFWQ---SSWKDRWVSVLNTDGSISFHNLEMGKSAPPEPIRIPAGNCGVPEPCDPLFIC 341
           ++ W     SW   W+                    P +P       C V   C  L  C
Sbjct: 270 QYTWDPQTQSWNMFWLQ-------------------PEDP-------CRVYNLCGQLGFC 329

Query: 342 YFD--NRCQCPSILNEKINCKIPSIP-CNGSSTELLYLGKDLDYFA----LRFSTPSFNS 401
             +    C C      + +    S    +G   E    G+  D F     LR+      S
Sbjct: 330 SSELLKPCACIRGFRPRNDAAWRSDDYSDGCRRENGDSGEKSDTFEAVGDLRYDGDVKMS 389

Query: 402 DL----NSCKAACAGNCSCHVLFFEPISGNC-FLFDQIGSLQRSEESSGGYISYMKTKLP 461
            L    +SC   C GN SC   + +  S  C  L +   +L+ S   +G     +  + P
Sbjct: 390 RLQVSKSSCAKTCLGNSSCVGFYHKEKSNLCKILLESPNNLKNSSSWTGVSEDVLYIREP 449

Query: 462 INGGD-AEMSPSQYRRRKHIVLMSILLAAMALAFMGLLCFRFYRRKMKELLSSIEDVTEE 521
             G     +S S       I+L S++ +   L F  L+     +R  K   +  +D   E
Sbjct: 450 KKGNSKGNISKS------IIILCSVVGSISVLGFTLLVPLILLKRSRKRKKTRKQD---E 509

Query: 522 DKFLEQISGGPMRFSFRQLRRATKNFSRKIGHGGFGSVYLGEV-GDGSRVAVKKLERMGQ 581
           D F          FSF++L+ AT  FS K+GHGGFG+V+ G + G  + VAVK+LER G 
Sbjct: 510 DGFAVL---NLKVFSFKELQSATNGFSDKVGHGGFGAVFKGTLPGSSTFVAVKRLERPGS 569

Query: 582 GAREFRAEVSLIGGIHHVNLVKLTGFCSESLHRLLVYEYMSNGSLDKWIFNGEEDAPFLD 641
           G  EFRAEV  IG I HVNLV+L GFCSE+LHRLLVY+YM  GSL  ++         L 
Sbjct: 570 GESEFRAEVCTIGNIQHVNLVRLRGFCSENLHRLLVYDYMPQGSLSSYL--SRTSPKLLS 629

Query: 642 WDTRFNIALGIARAVAYLHEECESKIIHCDIKPENVLLDQNFTPKVSDFGMAKLMDAQKS 701
           W+TRF IALG A+ +AYLHE C   IIHCDIKPEN+LLD ++  KVSDFG+AKL+    S
Sbjct: 630 WETRFRIALGTAKGIAYLHEGCRDCIIHCDIKPENILLDSDYNAKVSDFGLAKLLGRDFS 689

Query: 702 FICTQLRGTRGYVAPEWITHLAISDKTDVYSYGVLLLEIIAGRKSYHADS------EQPP 761
            +   +RGT GYVAPEWI+ L I+ K DVYS+G+ LLE+I GR++   +S      E  P
Sbjct: 690 RVLATMRGTWGYVAPEWISGLPITTKADVYSFGMTLLELIGGRRNVIVNSDTLGEKETEP 749

Query: 762 EKAHLPSYAMKMVAEQKGRRVLDPRLQVEEADRRVEAAVEVAVWCIQEEVSLRPPMRKVV 780
           EK   P +A + + +     V+D RL  E     V     VA+WCIQ+   +RP M  VV
Sbjct: 750 EKWFFPPWAAREIIQGNVDSVVDSRLNGEYNTEEVTRMATVAIWCIQDNEEIRPAMGTVV 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153573.10.0e+0098.95G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Momordica c... [more]
XP_022998179.10.0e+0078.13G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita m... [more]
XP_023523258.10.0e+0077.91G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita p... [more]
XP_022948682.10.0e+0077.67G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 [Cucurbita m... [more]
KAG6606798.10.0e+0075.76G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5, partial [Cu... [more]
Match NameE-valueIdentityDescription
Q8RWZ52.4e-20146.43G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops... [more]
O652383.3e-11834.31G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 OS=Arabi... [more]
O644772.7e-10434.66G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabi... [more]
Q9FLV46.0e-10433.45G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi... [more]
Q9XID39.3e-9732.28G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabi... [more]
Match NameE-valueIdentityDescription
A0A6J1DHU20.0e+0098.95G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Momordica... [more]
A0A6J1KDN30.0e+0078.13Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G9X70.0e+0077.67Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0KTK30.0e+0076.39Receptor-like serine/threonine-protein kinase OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A5D3E1U30.0e+0076.21Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT4G32300.11.7e-20246.43S-domain-2 5 [more]
AT5G35370.12.3e-11934.31S-locus lectin protein kinase family protein [more]
AT2G19130.11.9e-10534.66S-locus lectin protein kinase family protein [more]
AT1G34300.16.6e-9832.28lectin protein kinase family protein [more]
AT4G00340.19.8e-9433.80receptor-like protein kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 453..473
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 462..571
e-value: 2.2E-31
score: 109.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 572..774
e-value: 6.7E-61
score: 207.3
NoneNo IPR availablePANTHERPTHR47976:SF26G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-KINASE SD2-5coord: 2..819
NoneNo IPR availablePANTHERPTHR47976G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5coord: 2..819
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 503..771
e-value: 3.6849E-90
score: 283.395
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 40..155
e-value: 7.2E-19
score: 78.7
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 81..164
e-value: 6.9E-17
score: 61.8
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 34..153
score: 13.911236
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 44..155
e-value: 1.96747E-25
score: 99.6939
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 497..769
e-value: 3.2E-33
score: 126.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 500..767
e-value: 4.6E-50
score: 170.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 497..772
score: 37.007771
IPR024171S-receptor-like serine/threonine-protein kinasePIRSFPIRSF000641SRKcoord: 2..797
e-value: 1.9E-193
score: 642.9
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 38..153
e-value: 3.9E-14
score: 54.7
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 81..172
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 503..526
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 618..630
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 480..770

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013081.1MS013081.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity