MS012912 (gene) Bitter gourd (TR) v1

Overview


Name	MS012912
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold38: 398753 .. 399178 (-)
RNA-Seq Expression	MS012912
Synteny	MS012912

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGAGCGAAGACCAAAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCC

mRNA sequence

CTGAGCGAAGACCAAAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCC

Coding sequence (CDS)

CTGAGCGAAGACCAAAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCC

Protein sequence

LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWIREVDVGSDGRIRYEDFIARMVA

Homology

[Close]

BLAST of MS012912 vs. NCBI nr
Match: XP_022152721.1 (probable calcium-binding protein CML13 [Momordica charantia])

HSP 1 Score: 282.7 bits (722), Expect = 1.7e-72
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF
Sbjct: 5   LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REVDVGSDGRIRYEDFIARMVA
Sbjct: 125 REVDVGSDGRIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 274.2 bits (700), Expect = 6.1e-70
Identity = 134/142 (94.37%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LT+PFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDGRIRYEDFIARMVA
Sbjct: 125 REVEVGSDGRIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. NCBI nr
Match: XP_008448907.1 (PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML13 [Cucumis melo subsp. melo] >TYK19478.1 putative calcium-binding protein CML13 [Cucumis melo var. makuwa])

HSP 1 Score: 273.5 bits (698), Expect = 1.0e-69
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. NCBI nr
Match: XP_004147942.1 (probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetical protein Csa_011609 [Cucumis sativus])

HSP 1 Score: 272.3 bits (695), Expect = 2.3e-69
Identity = 134/142 (94.37%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. NCBI nr
Match: XP_023005593.1 (probable calcium-binding protein CML13 [Cucurbita maxima])

HSP 1 Score: 268.5 bits (685), Expect = 3.4e-68
Identity = 132/142 (92.96%), Postives = 139/142 (97.89%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFL++MSKHMKPE FDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VG DG+IRYEDFIARMVA
Sbjct: 125 REVEVGPDGKIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.1e-66
Identity = 123/142 (86.62%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+SPFDF
Sbjct: 6   LSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLSSPFDF 65

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKLEP+EFDEWI
Sbjct: 66  NRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNEFDEWI 125

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           +EVDVGSDG+IRYEDFIARMVA
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147

[Close]

BLAST of MS012912 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 3.6e-65
Identity = 120/142 (84.51%), Postives = 134/142 (94.37%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+SPFDF
Sbjct: 6   LSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLSSPFDF 65

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKL+PSEFDEWI
Sbjct: 66  NRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSEFDEWI 125

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           +EVDVGSDG+IRYEDFIARMVA
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147

[Close]

BLAST of MS012912 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-58
Identity = 109/142 (76.76%), Postives = 126/142 (88.73%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LSE+Q +SM+EAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LT+PFDF
Sbjct: 6   LSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAPFDF 65

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
            RFLDLM  H++PEPFDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFDEWI
Sbjct: 66  PRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFDEWI 125

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REVDV  DG IRY+DFI R+VA
Sbjct: 126 REVDVAPDGTIRYDDFIRRIVA 147

[Close]

BLAST of MS012912 vs. ExPASy Swiss-Prot
Match: P13868 (Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 5.1e-35
Identity = 71/144 (49.31%), Postives = 100/144 (69.44%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL--TSPF 60
           L+E+Q +  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I+E         
Sbjct: 5   LTEEQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMISEADADQNGTI 64

Query: 61  DFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDE 120
           DF  FL+LM++ MK    + +L++AFKV DKD  GF+  +ELRH++T++GEKL   E DE
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFKVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 WIREVDVGSDGRIRYEDFIARMVA 143
            IRE D+  DG++ YE+F+  M+A
Sbjct: 125 MIREADIDGDGQVNYEEFVRMMLA 148

[Close]

BLAST of MS012912 vs. ExPASy Swiss-Prot
Match: P93171 (Calmodulin OS=Helianthus annuus OX=4232 GN=CAM PE=2 SV=3)

HSP 1 Score: 148.7 bits (374), Expect = 5.1e-35
Identity = 74/144 (51.39%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL--TSPF 60
           L+EDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E         
Sbjct: 5   LTEDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  DFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDE 120
           DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   E DE
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 WIREVDVGSDGRIRYEDFIARMVA 143
            IRE DV  DG+I YE+F+  M+A
Sbjct: 125 MIREADVDGDGQINYEEFVKVMMA 148

[Close]

BLAST of MS012912 vs. ExPASy TrEMBL
Match: A0A6J1DIL4 (probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC111020377 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.3e-73
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF
Sbjct: 5   LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REVDVGSDGRIRYEDFIARMVA
Sbjct: 125 REVDVGSDGRIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. ExPASy TrEMBL
Match: A0A5D3D7C6 (Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001330 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.1e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. ExPASy TrEMBL
Match: A0A7G7Y0U3 (CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.1e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. ExPASy TrEMBL
Match: A0A1S3BLQ3 (probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.1e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. ExPASy TrEMBL
Match: A0A0A0L2F2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-69
Identity = 134/142 (94.37%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTSPFDF
Sbjct: 5   LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDF 64

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEFDEWI
Sbjct: 65  NRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEFDEWI 124

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           REV+VGSDG IRYEDFIARMVA
Sbjct: 125 REVEVGSDGSIRYEDFIARMVA 146

[Close]

BLAST of MS012912 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 253.8 bits (647), Expect = 8.0e-68
Identity = 123/142 (86.62%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS+DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+SPFDF
Sbjct: 6   LSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLSSPFDF 65

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKLEP+EFDEWI
Sbjct: 66  NRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNEFDEWI 125

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           +EVDVGSDG+IRYEDFIARMVA
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147

[Close]

BLAST of MS012912 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 248.8 bits (634), Expect = 2.6e-66
Identity = 120/142 (84.51%), Postives = 134/142 (94.37%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDF 60
           LS DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+SPFDF
Sbjct: 6   LSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLSSPFDF 65

Query: 61  NRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWI 120
           NRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKL+PSEFDEWI
Sbjct: 66  NRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSEFDEWI 125

Query: 121 REVDVGSDGRIRYEDFIARMVA 143
           +EVDVGSDG+IRYEDFIARMVA
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147

[Close]

BLAST of MS012912 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 147.9 bits (372), Expect = 6.2e-36
Identity = 73/144 (50.69%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL--TSPF 60
           L++DQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E         
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  DFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDE 120
           DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   E DE
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDE 124

Query: 121 WIREVDVGSDGRIRYEDFIARMVA 143
            IRE DV  DG+I YE+F+  M+A
Sbjct: 125 MIREADVDGDGQINYEEFVKVMMA 148

[Close]

BLAST of MS012912 vs. TAIR 10
Match: AT3G43810.1 (calmodulin 7 )

HSP 1 Score: 147.5 bits (371), Expect = 8.1e-36
Identity = 73/144 (50.69%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL--TSPF 60
           L++DQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E         
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  DFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDE 120
           DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   E DE
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 WIREVDVGSDGRIRYEDFIARMVA 143
            IRE DV  DG+I YE+F+  M+A
Sbjct: 125 MIREADVDGDGQINYEEFVKVMMA 148

[Close]

BLAST of MS012912 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 146.4 bits (368), Expect = 1.8e-35
Identity = 72/144 (50.00%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 1   LSEDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL--TSPF 60
           L++DQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E         
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  DFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDE 120
           DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   E DE
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 WIREVDVGSDGRIRYEDFIARMVA 143
            I+E DV  DG+I YE+F+  M+A
Sbjct: 125 MIKEADVDGDGQINYEEFVKVMMA 148

The following BLAST results are available for this feature:

BLAST of MS012912 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152721.1	1.7e-72	99.30	probable calcium-binding protein CML13 [Momordica charantia]	[more]
XP_038876882.1	6.1e-70	94.37	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_008448907.1	1.0e-69	95.07	PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML...	[more]
XP_004147942.1	2.3e-69	94.37	probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetica...	[more]
XP_023005593.1	3.4e-68	92.96	probable calcium-binding protein CML13 [Cucurbita maxima]	[more]

BLAST of MS012912 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	1.1e-66	86.62	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	3.6e-65	84.51	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	1.1e-58	76.76	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P13868	5.1e-35	49.31	Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2	[more]
P93171	5.1e-35	51.39	Calmodulin OS=Helianthus annuus OX=4232 GN=CAM PE=2 SV=3	[more]

BLAST of MS012912 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIL4	8.3e-73	99.30	probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3D7C6	5.1e-70	95.07	Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A7G7Y0U3	5.1e-70	95.07	CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1	[more]
A0A1S3BLQ3	5.1e-70	95.07	probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 P...	[more]
A0A0A0L2F2	1.1e-69	94.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1	[more]

BLAST of MS012912 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	8.0e-68	86.62	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	2.6e-66	84.51	Calcium-binding EF-hand family protein	[more]
AT5G21274.1	6.2e-36	50.69	calmodulin 6	[more]
AT3G43810.1	8.1e-36	50.69	calmodulin 7	[more]
AT2G41110.1	1.8e-35	50.00	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 79..107 e-value: 5.3E-4 score: 29.3 coord: 8..36 e-value: 8.4E-5 score: 31.9 coord: 115..142 e-value: 18.0 score: 7.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 78..140 e-value: 1.9E-14 score: 53.8
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 9..37 e-value: 3.4E-6 score: 26.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 13.062845
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 75..110 score: 12.253878
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 10..51 e-value: 9.31253E-9 score: 47.1573
None	No IPR available	GENE3D	1.10.238.10		coord: 77..141 e-value: 2.8E-19 score: 71.0
None	No IPR available	GENE3D	1.10.238.10		coord: 1..76 e-value: 1.2E-19 score: 72.2
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..142
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..142
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 1..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012912.1	MS012912.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding